Skin photoaging repairing composition as well as preparation method and application thereof

Abstract

The embodiment of the invention provides a skin photoaging repairing composition as well as a preparation method and application thereof. The repairing composition comprises the following components in percentage by weight: 1%-20% of naringenin; 0.1% to 10% of ergothioneine; 0.5% to 10% of thiotaurine; 1%-15% of nicotinamide; 1%-15% of resveratrol; based on the total weight of the repair composition. According to the repairing composition provided by the embodiment of the invention, intervention can be carried out from multiple target points based on deep reasons of light aging related to multi-way injury, the obvious anti-oxidation, anti-inflammatory and DNA protection effects are achieved, DNA injury induced by UVB can be effectively inhibited, overexpression of inflammatory factors such as IL-1beta, IL-6, MMP-1, TNF-alpha and MMP-9 is reduced, skin edema is relieved, and the repairing composition has the advantages of being free of toxic and side effects, free of side effects and the like. And the level of senescence markers is reduced, so that the photoaging phenomenon of the skin is obviously improved.

Description

Technical Field

[0001] The embodiments of the present application relate to the technical field of daily cosmetics, and specifically to a skin photoaging repair composition, a preparation method, and uses thereof. Background Art

[0002] As the body's largest outer protective barrier, the skin is extensively exposed to a changing external environment and is constantly challenged by various external factors. Skin aging is a complex and multidimensional physiological process, a natural consequence of the passage of time and the combined effects of numerous internal and external stimuli.

[0003] For example, when it comes to extrinsic aging, ultraviolet radiation and environmental pollution are two of the most significant contributing factors. Specifically, acute UVB radiation (wavelength range between 290nm and 320nm) can significantly damage skin structure, leading to a decrease in sodium hyaluronate (HA) content in the dermis and epidermis, and subsequently causing skin moisture loss. This process is often accompanied by the accumulation of abnormal elastin fibers and the degradation of collagen, ultimately causing a series of skin problems, including but not limited to deeper wrinkles, rough skin texture, skin sagging, increased pigmentation, and the formation of photoaging freckles. More seriously, UVB radiation can also trigger skin inflammation, promote oxidative stress, and cause DNA damage, which can lead to skin cancer. In addition to solar radiation, air pollution is also a major factor in skin aging. Harmful substances in the air, such as particulate matter, smoke, and nitrogen dioxide, as well as components in tobacco smoke, can activate the expression of MMP1 in human fibroblasts and keratinocytes in the skin, thereby accelerating the degradation of the skin matrix and promoting accelerated skin aging.

[0004] In the existing technology, relevant research is exploring how various drugs can act on the specific channels and targets of ultraviolet rays and air pollution particles to achieve the effect of preventing and treating skin aging. However, prevention is still the main means of delaying photoaging. Although some treatment methods exist, such as chemical peels and laser therapy, these treatment methods have some effect on improving symptoms such as chloasma, sagging, and enlarged pores caused by photoaging, but these treatment methods are accompanied by a series of significant side effects, including but not limited to erythema, allergies, burns, scalds, thermal damage, dry skin, peeling, increased dandruff, itching, tissue necrosis, etc.

[0005] Therefore, it is urgent to develop safer and more effective products to treat skin photoaging. Summary of the Invention

[0006] The purpose of the embodiments of the present application is to provide a skin photoaging repair composition and its preparation method and use.

[0007] To achieve the above objectives, the present application proposes the following technical solutions: In a first aspect, the present invention provides a composition for repairing skin photoaging, wherein the composition comprises, by weight percentage: Naringenin: 1%~20%; Ergothioneine: 0.1%~10%; Thiotaurine: 0.5%~10%; Niacinamide: 1%~15%; Resveratrol: 1%~15%; Based on the total weight of the repair composition.

[0008] As an embodiment, the repair composition comprises: Naringenin: 5%~10%; Ergothioneine: 0.1%~2%; Thiotaurine: 0.5%~5%; Niacinamide: 1%~5%; Resveratrol: 1%~5%; Based on the total weight of the repair composition.

[0009] As an embodiment, the repair composition further comprises at least one cosmetic additive.

[0010] As an embodiment, the cosmetic additive is selected from at least one of betaine and butylene glycol.

[0011] In a second aspect, the present invention provides a method for preparing the skin photoaging repair composition as described in the first aspect, the preparation method comprising: Weigh each component in proportion, and stir the components evenly at 40-60° C. to obtain a repair composition.

[0012] In a third aspect, the embodiments of the present application propose the use of the skin photoaging repair composition described in the first aspect in the preparation of a product for improving and / or alleviating UVB-induced skin photoaging symptoms.

[0013] As an embodiment, the skin photoaging symptom is at least one of sunburn, erythema, peeling, pigmentation, dry skin, loose skin or rough skin.

[0014] In a fourth aspect, the examples of the present application propose the use of a combination of naringenin, ergothioneine, thiotaurine, niacinamide and resveratrol in preparing a composition for repairing skin photoaging.

[0015] As an embodiment, in the repair composition, the content of naringenin is 1% to 20%, the content of ergothioneine is 0.1% to 10%, the content of thiotaurine is 0.5% to 10%, the content of niacinamide is 1% to 15%, and the content of resveratrol is 1% to 15%, based on the total weight of the repair composition.

[0016] As an embodiment, in the repair composition, the content of naringenin is 5% to 10%, the content of ergothioneine is 0.1% to 2%, the content of thiotaurine is 0.5% to 5%, the content of niacinamide is 1% to 5%, and the content of resveratrol is 1% to 5%, based on the total weight of the repair composition.

[0017] The embodiments of the present application have at least the following beneficial effects: In the present embodiment, ergothioneine, thiotaurine, nicotinamide, and resveratrol are added to naringenin to obtain a photoaging repair composition. Among them, ergothioneine has excellent antioxidant and anti-inflammatory effects, can protect mitochondria from free radical damage, and can protect the skin and reduce the oxidative stress caused by UVB in combination with naringenin. Thiotaurine can repair damage to cells and work together with naringenin to repair photoaging damage caused by UVB. Nicotinamide has whitening and anti-aging effects and can work together with naringenin to repair, whiten, and soothe sun-exposed skin. Resveratrol has good whitening effects and can enhance the whitening effect when combined with naringenin.

[0018] Therefore, the repair composition provided in the embodiments of the present application, based on the deep-seated cause of photoaging involving multi-pathway damage, can intervene from multiple targets, has significant antioxidant, anti-inflammatory and DNA protection effects, can effectively inhibit UVB-induced DNA damage, reduce the overexpression of inflammatory factors such as IL-1β, IL-6, MMP-1, TNF-α, MMP-9, reduce skin edema, and reduce the level of aging markers, thereby significantly improving the phenomenon of skin photoaging.

[0019] Additional aspects and advantages of the present application will be given in part in the following description, which will become apparent from the following description, or will be learned through practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A schematic diagram showing the effect of the anti-photoaging emulsion in Example 2 on the inflammatory factor IL-1β in photoaged skin of mice; Figure 2 A schematic diagram showing the effect of the anti-photoaging emulsion in Example 2 on the inflammatory factor IL-6 in photoaged skin of mice; Figure 3A schematic diagram showing the effect of the anti-photoaging emulsion in Example 2 on the inflammatory factor MMP-1 in photoaged skin of mice; Figure 4 A schematic diagram showing the effect of the anti-photoaging emulsion in Example 2 on the inflammatory factor MMP-9 in photoaged skin of mice; Figure 5 A schematic diagram showing the effect of the anti-photoaging emulsion in Example 2 on the inflammatory factor TNF-α in photoaged skin of mice; Figure 6 A schematic diagram showing the effect of the anti-photoaging emulsion in Example 3 on the skin weight of photoaged mouse skin is shown. DETAILED DESCRIPTION

[0021] The following will be combined with the embodiments of the present application and the accompanying drawings to clearly and completely describe the technical solutions in the embodiments. Obviously, the embodiments described below are only some of the embodiments of the present application, rather than all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0022] It will be understood that when used in this specification and the appended claims, the terms “comprises” and “comprising” indicate the presence of described features, integers, steps, operations, elements and / or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or groups thereof.

[0023] It should also be understood that the terms used in this specification of the embodiments of the present application are only for the purpose of describing specific embodiments and are not intended to limit the embodiments of the present application. As used in the specification of the embodiments of the present application and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms unless the context clearly indicates otherwise.

[0024] The skin photoaging repair composition of this embodiment, its preparation method and use will be described in detail below.

[0025] First, the skin photoaging repair composition according to the first aspect of this embodiment is described.

[0026] Skin photoaging repair composition Currently, there are relatively few safe and effective anti-photoaging products, all with limited functionality. Few comprehensive compositions exist for preventing, treating, or repairing photoaging. Naringenin (5,7,4'-trihydroxyflavanone, Naringenin, NAR) is a polyphenolic flavonoid compound derived from the hydrolysis of naringin, one molecule of rhamnose and one molecule of glucose. Its structural formula is shown below: ;

[0027] Currently, thanks to its typical flavonoid structure, naringenin exhibits typical physiological activities of flavonoids, such as vasodilation, antibacterial, and anti-allergic effects. In addition, the presence of three phenolic hydroxyl groups and an aromatic ring conjugated structure in the naringenin molecule also gives naringenin excellent UVB absorption capacity. This property gives naringenin broad application prospects in the treatment and prevention of photoaging. However, photoaging involves multiple pathways of damage, and the use of naringenin alone for single-target intervention has limited effect. For example: In view of this, this embodiment provides a skin photoaging repair composition, which includes, by weight percentage, naringenin: 1% to 20%; ergothioneine: 0.1% to 10%; thiotaurine: 0.5% to 10%; niacinamide: 1% to 15%; and resveratrol: 1% to 15%; based on the total weight of the repair composition.

[0028] In this embodiment, ergothioneine, thiotaurine, nicotinamide and resveratrol are added to naringenin to obtain a photoaging repair composition. The repair composition of this embodiment has significant antioxidant, anti-inflammatory and DNA protection effects, can effectively inhibit UVB-induced DNA damage, reduce the overexpression of inflammatory factors such as IL-1β, IL-6, MMP-1, TNF-α, MMP-9, reduce skin edema, and reduce the level of aging markers, thereby significantly improving the phenomenon of skin photoaging. Specifically, the principle of this application may be: When ultraviolet rays (especially UVB) irradiate the skin, they induce the production of a large number of reactive oxygen species (ROS), such as superoxide anions and hydroxyl radicals. These free radicals attack the DNA, proteins and lipids in skin cells, leading to cell damage and aging.

[0029] In the above composition, first, ergothioneine has excellent antioxidant and anti-inflammatory effects, and can protect mitochondria from free radical damage; specifically, ergothioneine can, together with naringenin, effectively scavenge free radicals induced by ultraviolet rays, reduce the damage of oxidative stress to the skin, and protect cells from oxidative damage; at the same time, ergothioneine can also, together with naringenin, inhibit the production of inflammatory factors (such as IL-6, TNF-α), reduce ultraviolet-induced inflammatory reactions, and thus protect the skin from inflammation-mediated damage.

[0030] Secondly, thiotaurine can protect mitochondria from oxidative damage by enhancing the antioxidant capacity of cell membranes, while promoting cell metabolism and enhancing cell proliferation. Ergothioneine can protect mitochondria from free radical attacks and maintain the normal function of mitochondria; naringenin, as a mitochondrial potassium channel activator, can increase mitochondrial activity, provide energy support for cell repair, and at the same time repair mitochondria damaged by UVB and restore the integrity of the mitochondrial membrane. The combined effect of the three can significantly improve mitochondrial function, promote cellular energy metabolism, and accelerate the repair and regeneration of photoaged cells. At the same time, naringenin can reduce ultraviolet-induced extracellular matrix degradation by inhibiting the expression of MMPs; and thiotaurine can further promote the production of beneficial substances (such as taurine and hypotaurine), enhance cell proliferation, and further reduce cell damage caused by photoaging. Based on the combined effect of thiotaurine and naringenin, it can significantly promote the repair and regeneration of photoaged cells and repair photoaging damage caused by UVB.

[0031] Furthermore, this embodiment further adds nicotinamide, which can not only inhibit the expression of inflammatory factors (such as IL-8, TNF-α), reduce redness and swelling after sun exposure, but also block the transfer of melanosomes from melanocytes to keratinocytes, reducing the formation of spots. As mentioned above, naringenin can not only activate mitochondrial potassium ion channels, scavenge free radicals, inhibit MAPK signaling pathways (such as p-JNK, p-p38), reduce the release of inflammatory factors (such as IL-6, TNF-α), but also inhibit tyrosinase activity and reduce melanin synthesis. Therefore, nicotinamide and naringenin can not only dually inhibit melanin from the source (synthesis) to the terminal (transfer), achieving a more efficient whitening effect, but also based on a dual antioxidant mechanism, can promote cell proliferation, accelerate barrier repair, and strengthen the skin barrier.

[0032] Among them, the skin after barrier repair can better absorb naringenin, allowing it to act more effectively on the dermis to further improve the phenomenon of skin photoaging.

[0033] Finally, resveratrol can not only directly neutralize UV-induced reactive oxygen species and reduce oxidative stress damage to skin cells; it can also inhibit the release of UV-induced inflammatory factors (such as TNF-α and IL-6), reducing inflammatory responses. At the same time, resveratrol can inhibit the activity of tyrosinase, thereby reducing the production of melanin, and can promote the repair and regeneration of photoaged cells by inhibiting the activity of UV-induced matrix metalloproteinases (MMPs). Therefore, resveratrol can further enhance the antioxidant, anti-inflammatory, whitening, and skin (barrier) repair effects of the repair composition on the basis of naringenin, ergothioneine, thiotaurine, and niacinamide.

[0034] In summary, the repair composition provided in this embodiment, based on the deep-seated cause of photoaging involving multi-pathway damage, can intervene from multiple targets, has significant antioxidant, anti-inflammatory and DNA protection effects, can effectively inhibit UVB-induced DNA damage, reduce the overexpression of inflammatory factors such as IL-1β, IL-6, MMP-1, TNF-α, MMP-9, alleviate skin edema, and reduce the levels of aging markers, thereby significantly improving the phenomenon of skin photoaging.

[0035] As a preferred embodiment, the repair composition comprises: naringenin: 5% to 10%; ergothioneine: 0.1% to 2%; thiotaurine: 0.5% to 5%; niacinamide: 1% to 5%; and resveratrol: 1% to 5%; based on the total weight of the repair composition.

[0036] Illustratively, in the repair composition of this embodiment: The content of naringenin includes but is not limited to: 1.0%, 1.3%, 1.6%, 1.7%, 1.8%, 1.88%, 1.9%, 1.97%, 2.0%, 2.3%, 2.5%, 2.7%, 2.8%, 2.9%, 3.0%, 4.0%, 4.1%, 4.15%, 4.2%, 4.28%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5%, 5.1%, 5.2%, 5.5 %, 5.8%, 6%, 6.4%, 7%, 7.3%, 7.4%, 7.8%, 8%, 8.3%, 8.6%, 9%, 9.3%, 9.5%, 10%, 11%, 12%, 13%, 13.5%, 14%, 14.5%, 15%, 15.5%, 16%, 16.5%, 17%, 17.2%, 17.6%, 18%, 18.6%, 18.8%, 19%, 19.2%, 19.5%, 19.8%, 20%.

[0037] The content of ergothioneine includes but is not limited to: 0.1%, 0.12%, 0.15%, 0.19%, 0.2%, 0.22%, 0.24%, 0.3%, 0.37%, 0.39%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.3%, 1.6%, 1.7%, 1.8%, 1.88%, 1.9%, 1.97%, 2.0%, 2.3%, 2.5%, 2 .7%, 2.8%, 2.9%, 3.0%, 4.0%, 4.1%, 4.15%, 4.2%, 4.28%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5%, 5.1%, 5.2%, 5.5%, 5.8%, 6%, 6.4%, 7%, 7.3%, 7.4%, 7.8%, 8%, 8.3%, 8.6%, 9%, 9.3%, 9.5%, 10%.

[0038] The content of thiotaurine includes, but is not limited to, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.3%, 1.6%, 1.7%, 1.8%, 1.88%, 1.9%, 1.97%, 2.0%, 2.3%, 2.5%, 2.7%, 2.8%, 2.9%, 3.0%, 4.0%, 4.1%, 4.15%, 4.2%, 4.28%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5%, 5.1%, 5.2%, 5.5%, 5.8%, 6%, 6.4%, 7%, 7.3%, 7.4%, 7.8%, 8%, 8.3%, 8.6%, 9%, 9.3%, 9.5%, and 10%.

[0039] The content of niacinamide includes but is not limited to: 1.0%, 1.3%, 1.6%, 1.7%, 1.8%, 1.88%, 1.9%, 1.97%, 2.0%, 2.3%, 2.5%, 2.7%, 2.8%, 2.9%, 3.0%, 4.0%, 4.1%, 4.15%, 4.2%, 4.28%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5%, 5.1%, 5.2%, 5.5%, 5.8%, 6%, 6.4%, 7%, 7.3%, 7.4%, 7.8%, 8%, 8.3%, 8.6%, 9%, 9.3%, 9.5%, 10%, 11%, 12%, 13%, 13.5%, 14%, 14.5%, 15%.

[0040] The amount of resveratrol includes, but is not limited to, 1.0%, 1.3%, 1.6%, 1.7%, 1.8%, 1.88%, 1.9%, 1.97%, 2.0%, 2.3%, 2.5%, 2.7%, 2.8%, 2.9%, 3.0%, 4.0%, 4.1%, 4.15%, 4.2%, 4.28%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5%, 5.1%, 5.2%, 5.5%, 5.8%, 6%, 6.4%, 7%, 7.3%, 7.4%, 7.8%, 8%, 8.3%, 8.6%, 9%, 9.3%, 9.5%, 10%, 11%, 12%, 13%, 13.5%, 14%, 14.5%, 15%.

[0041] It should be understood that the repair composition of this embodiment can be prepared into a cosmetic composition of the corresponding dosage form by adding other components conventionally used in the art (i.e., at least one cosmetic additive other than the above components) to the above-mentioned components (naringenin, ergothioneine, thiotaurine, niacinamide, and resveratrol). For example, other components conventionally used in the art can be reasonably used within the scope that does not significantly impair the effects of this embodiment.

[0042] The types and contents of other components can be adjusted according to the type of product to be manufactured.

[0043] For example, any ingredients known in the art, such as vehicles, surfactants, skin care active ingredients, and cosmetic excipients, can be used, and their types and amounts can be selected according to specific needs. Typically, the content of other ingredients is 10-80% by weight, based on the total weight of the repair composition.

[0044] In this embodiment, the above-mentioned vehicle is known in the art, for example, including but not limited to diluents, dispersants or carriers, including but not limited to ethanol, butanediol, 1,2-hexanediol, dipropylene glycol, etc. Generally, the above-mentioned vehicle accounts for 1-25% of the total weight of the other components.

[0045] In this embodiment, the surfactant is any type of surfactant commonly used in cosmetics. The surfactant includes, but is not limited to, fatty acid soaps (such as sodium laurate, sodium palmitate, etc.), higher fatty acid amide sulfonates (such as sodium lauryl methyl taurate, etc.), alkylbenzene sulfonates, higher fatty acid ester sulfates (such as hardened coconut oil fatty acid glycerol sulfate, etc.), N-acyl glutamates, lauryl dimethylaminoacetic acid betaine, alkyl betaines, amido betaines, PEG-alkyl ethers (such as PEG-2-octyl dodecyl ether, etc.), sucrose fatty acid esters, etc. One or more. Typically, the surfactant accounts for 1-20% of the total weight of the other components.

[0046] It will be appreciated that the skin care active ingredients are also known in the art and include, but are not limited to, moisturizers, skin conditioners, emollients, and the like. For example, the moisturizers include, but are not limited to, one or more of betaine, glycerin, trehalose, sucrose, panthenol, propylene glycol, 1,2-pentanediol, 1,2-hexanediol, mannitol, urea, hydrolyzed sclerotium gum, sodium polyglutamate, and glycerol glucoside. Typically, the moisturizers comprise 1-50% of the total weight of the other components.

[0047] The emollients include, but are not limited to, one or more of cetyl alcohol, dimethicone, caprylic / capric triglyceride, ethylhexyl isononanoate, stearyl alcohol, isopropyl myristate, myristyl alcohol, lanolin, paraffin, microcrystalline wax, beeswax, etc. Typically, the emollients account for 0.05-20% of the total weight of the other components.

[0048] Among them, cosmetic excipients can be selected from conventional excipients such as emulsifiers, thickeners, preservatives, and fragrances.

[0049] For example, the emulsifiers include, but are not limited to, one or more of sorbitan olivate, steareth-21, PEG-60 hydrogenated castor oil, glyceryl oleate citrate, PPG-13-decyltetradecyl alcohol polyether-24, and cetearyl glucoside. The thickeners include, but are not limited to, one or more of ammonium acryloyldimethyltaurate / steareth-8 methacrylate copolymer, hydroxyethyl cellulose, hydroxypropyl cellulose, carbomer, xanthan gum, gum arabic, and ammonium acryloyldimethyltaurate / VP copolymer. The preservatives include, but are not limited to, one or more of methylparaben, propylparaben, phenoxyethanol, benzyl alcohol, phenylethyl alcohol, potassium sorbate, and sodium benzoate, or ingredients with preservative properties not listed in the preservatives list, such as one or more of p-hydroxyacetophenone, glyceryl caprylate, capryloylhydroxamic acid, pentylene glycol, and hexylene glycol. Typically, the preservatives comprise 0-1.5% of the total weight of the other components.

[0050] It is understandable that the repair composition of this embodiment can be made into conventional dosage forms such as lotions, essences, emulsions, ointments, creams, gels, etc.

[0051] Next, the preparation method of the second aspect of this embodiment will be described.

[0052] Preparation method The repair composition of this embodiment can be prepared by any suitable method known in the art.

[0053] For example: use conventional containers such as dissolution tanks, emulsification pots, and dispersers commonly used in this field for preparation. During preparation, first put the water-soluble substance into the water-phase dissolution kettle, and put the oil-soluble substance into the oil-phase dissolution kettle, and heat the temperature of the two kettles to about 40-60°C respectively. For raw materials that are easy to agglomerate, they can be pre-dispersed with a disperser. After the dissolution is completed, the oil phase and the water phase are transported to the emulsification pot and homogenized for about 10-30 minutes. After the emulsification is completed, the temperature of the material is lowered to 40°C, and flavors (if any), preservatives (if any), pH adjusters, etc. are optionally added. After the relevant test indicators are qualified, it can be filled and shipped.

[0054] Specifically, the preparation method of the repair composition of this embodiment includes: Weigh each component in proportion, and stir the components evenly at 40-60° C. to obtain a repair composition.

[0055] Next, the use of the third aspect of this embodiment will be described.

[0056] use This embodiment further proposes the use of the skin photoaging repair composition described in the first aspect in the preparation of a product for improving and / or alleviating UVB-induced skin photoaging symptoms.

[0057] Based on the description of the first aspect, the repair composition of this embodiment has significant antioxidant, anti-inflammatory and DNA protection effects, can effectively inhibit UVB-induced DNA damage, reduce the overexpression of inflammatory factors such as IL-1β, IL-6, MMP-1, TNF-α, MMP-9, reduce skin edema, and reduce the level of aging markers, thereby significantly improving the phenomenon of skin photoaging.

[0058] Therefore, the product prepared in this embodiment also has corresponding effects. For example, the product can be used to improve and / or alleviate at least one of the symptoms of photoaging of the skin, including sunburn, erythema, peeling, pigmentation, dry skin, loose skin, or rough skin.

[0059] The products prepared in this embodiment include, but are not limited to, lotions, essences, emulsions, ointments, creams or gels.

[0060] Hereinafter, the use of the combination of naringenin, ergothioneine, thiotaurine, niacinamide and resveratrol in the preparation of a skin photoaging repair composition according to the fourth aspect of this embodiment will be described.

[0061] Use in preparing skin photoaging repair composition Specifically, this example proposes the use of a combination of naringenin, ergothioneine, thiotaurine, niacinamide, and resveratrol in preparing a skin photoaging repair composition.

[0062] Referring to the description of the first aspect, this embodiment adds ergothioneine, thiotaurine, niacinamide, and resveratrol to naringenin to produce a photoaging repair composition. The repair composition of this embodiment has significant antioxidant, anti-inflammatory, and DNA protective effects, effectively inhibiting UVB-induced DNA damage, reducing the overexpression of inflammatory factors such as IL-1β, IL-6, MMP-1, TNF-α, and MMP-9, alleviating skin edema, and lowering the levels of aging markers, thereby significantly improving skin photoaging.

[0063] Based on the description of the first aspect, the specific principles of the photoaging repair composition will not be further discussed here.

[0064] As an embodiment, in the repair composition, the content of naringenin is 1% to 20%, the content of ergothioneine is 0.1% to 10%, the content of thiotaurine is 0.5% to 10%, the content of niacinamide is 1% to 15%, and the content of resveratrol is 1% to 15%, based on the total weight of the repair composition.

[0065] As a preferred embodiment, in the repair composition, the content of naringenin is 5% to 10%, the content of ergothioneine is 0.1% to 2%, the content of thiotaurine is 0.5% to 5%, the content of niacinamide is 1% to 5%, and the content of resveratrol is 1% to 5%, based on the total weight of the repair composition.

[0066] It should be understood that the repair composition of this embodiment can be prepared into a cosmetic composition of the corresponding dosage form by adding other components conventionally used in the art (i.e., at least one cosmetic additive other than the above components) to the above-mentioned components (naringenin, ergothioneine, thiotaurine, niacinamide, and resveratrol). For example, other components conventionally used in the art can be reasonably used within the scope that does not significantly impair the effects of this embodiment.

[0067] The specific selection of cosmetic additives can be adjusted according to the needs of different dosage forms. The cosmetic additives in this embodiment can refer to the relevant description of the cosmetic composition of the first aspect.

[0068] The present application will be further described below with reference to specific examples. It should be understood that these examples are only used to illustrate / explain the present application and are not intended to limit the scope of the present application.

[0069] In the following examples, all materials, reagents and instruments used can be purchased from commercial sources unless otherwise specified.

[0070] In the following examples, SIMULGEL™ EG is an existing thickening and stabilizing agent, and its ingredients include ethylhexylglycerin sodium acrylate / sodium acryloyldimethyl taurate copolymer, isohexadecane, and polysorbate 80.

[0071] Example 1 In this embodiment, repair compositions having the following formulas were prepared, and the specific compositions are shown in Table 1: Table 1: Composition and content of compositions 1-10 (g)

[0072] Among them, compositions 6-9 are the experimental groups of this example, and the remaining compositions are the control groups.

[0073] The preparation method of the above-mentioned repair composition comprises: weighing each component in proportion, and stirring each component uniformly at 45° C. to obtain the repair composition.

[0074] Next, the effects of the above composition on the activity of mouse cells will be tested.

[0075] The specific experimental steps include: taking mouse dermal fibroblasts, digesting them with trypsin, and resuspending them in complete medium to prepare a cell concentration of 5×10 3 / mL of cell suspension was inoculated into a 96-well plate, 100μL per well. After culturing in a 37°C incubator for 24h, the above composition was added with distilled water to prepare 200μL of the test solution with three different concentrations of 50μg / mL, 100μg / mL, and 200μg / mL, and treated with mouse dermal fibroblast complete culture medium for 72h. At the same time, a blank group without the composition and only distilled water was set up. The culture medium was subjected to 48h, 10mJ / cm 2 UVB irradiation was performed. After irradiation, the cell culture medium was discarded, the cells were washed with PBS, and the cells were stained according to the instructions of the β-galactoside staining kit (Sigma-Aldrich) for observation. A control group was not irradiated with UVB and only received distilled water without the composition. Positive cells were identified as senescent cells. The test results are shown in Tables 2 and 3: Table 2: Percentage of positively stained cells in the control group and blank group (%) Table 3: Percentage of positively stained cells for compositions 1-10 (%)

[0076] Please refer to Table 2. Compared with the control group, the percentage of positively stained cells in the blank group was significantly increased, indicating that the model was successfully established. Please continue to refer to Table 3. Compared with the model group, in compositions 1-5, naringenin, ergothioneine, thiotaurine, nicotinamide or resveratrol alone can reduce the percentage of β-galactosidase-positive cells in mouse dermal fibroblasts, indicating that these ingredients have a certain effect of resisting UVB-induced aging of dermal fibroblasts; among them, naringenin has the best anti-photoaging effect. However, the anti-photoaging effects of the above components used alone are obviously insufficient.

[0077] Specifically, among compositions 6-9, the composition prepared using naringenin and ergothioneine, thiotaurine, nicotinamide, and resveratrol can significantly reduce the proportion of cells positive for β-galactosidase staining, and the effect becomes better as the concentration of the composition increases.

[0078] Furthermore, composition 10 reduces naringenin compared to composition 6, and its anti-photoaging effect is significantly reduced compared to composition 6.

[0079] In summary, the composition prepared by combining naringenin with ergothioneine, thiotaurine, niacinamide, and resveratrol in this example has a significant effect of resisting the aging of dermal fibroblasts irradiated by UVB, and the effect is better than the effect of using the components alone.

[0080] Example 2: Establishment and testing of a photoaging mouse model.

[0081] 2.1. Mouse breeding methods Forty-eight KM mice aged 6 to 8 weeks were housed in a clean, standard animal room with a relative humidity of 30% to 60%, a 12h / 12h light / dark cycle, and a temperature of 24±2°C. They were given free access to food and water. All animal experiments in this example were conducted in accordance with animal protocols.

[0082] 2.2. Mouse Grouping and Dosing Mice were divided into six groups (n=4): normal control (NC), UVB irradiation group, pre-blank + UVB group, pre-drug + UVB group, UVB + blank group, and UVB + drug group. The six groups of mice were acclimated for 7 days. The day before the experiment, a 5.0 cm × 5.0 cm area of the mouse back hair was shaved (hair was shaved every 7 days during the experiment). The shaving technique was controlled to avoid affecting the test skin on the mouse back and to control for interfering factors.

[0083] Among them, the configuration of the relevant emulsion is: Blank emulsion: 5 g 1,2-propylene glycol, 0.2 g carbomer, 0.03 g EDTA, 0.5 g SIMULGEL™ EG, 1.3 g ethylhexyl palmitate, 1.2 g caprylic / capric triglyceride, and 91.77 g deionized water. Place the above materials in a beaker, heat to 55°C in a water bath, stir at 300 rpm for 15 min, and let stand for 15 min to obtain a blank emulsion.

[0084] Anti-photoaging emulsion: 5 g 1,2-propylene glycol, 0.2 g carbomer, 0.03 g EDTA, 0.5 g SIMULGEL™ EG, 1.3 g ethylhexyl palmitate, 1.2 g caprylic / capric triglyceride, 15 g naringenin composition (consisting of 10% naringenin, 1% ergothioneine, 1% thiotaurine, 5% niacinamide, 5% resveratrol, 40% betaine, 15% butylene glycol, and 23.9% deionized water), and 76.77 g deionized water. Place the above materials in a beaker, heat to 55°C in a water bath, stir at 300 rpm for 15 minutes, and let stand for 15 minutes to obtain the anti-photoaging emulsion.

[0085] Among them, the normal control group was raised normally and was not irradiated with a UVB irradiator; the UVB irradiation group was irradiated with a UVB irradiator (USA SPECTRONICS) that had been preheated for 15 minutes for 30 minutes per time; the mice in the pre-drug + UVB group (hereinafter referred to as the pre-drug group) were first evenly smeared with 1 mL of the above-prepared anti-photoaging emulsion, and then absorbed for 15 minutes before irradiation with a UVB irradiator; the mice in the pre-blank + UVB group (hereinafter referred to as the pre-blank group) were evenly smeared with 1 mL of the above-prepared blank emulsion, and then absorbed for 15 minutes before irradiation with a UVB irradiator; the mice in the UVB + blank group (hereinafter referred to as the blank group) were first irradiated with a UVB irradiator for 30 minutes and then smeared with 1 mL of blank emulsion; the mice in the UVB + drug administration group (hereinafter referred to as the drug administration group) were first irradiated with a UVB irradiator for 30 minutes and then smeared with 1 mL of anti-photoaging emulsion.

[0086] The subjects were irradiated for a total of 9 weeks. In the first week, the subjects were given 1×MED and irradiated every other day (3 times / week). In the second week, the subjects were given 2×MED and irradiated every other day (3 times / week). In the third week, the subjects were given 3×MED and irradiated every other day (3 times / week). From the fourth to the ninth week, the subjects were given 4×MED and irradiated every other day (3 times / week). Except for the blank control group and the UVB irradiation group, the other groups also applied the corresponding lotion on non-irradiation days.

[0087] The skin appearance of the backs of the six groups of mice was observed. 48 hours after the last UVB radiation exposure, the experimenters scored the degree of skin damage in the irradiated areas of the backs of the six groups of mice. The scoring criteria are shown in Table 4, and the scoring results are shown in Table 5:

[0088] Table 4: Mouse skin damage assessment table Table 5: Effects of naringenin on photoaged skin in mice

[0089] Note: In Table 5, #p<0.05 compared with the normal group; *p<0.05 or **p<0.01 compared with the UVB irradiation group.

[0090] Detection of biochemical indicators in mouse skin tissue After scoring, mice in each group were sacrificed, and skin tissue was rapidly isolated. Skin samples were homogenized in 0.5% HATB and 0.05M K₂HPO₄ buffer (pH 6.0). After centrifugation at 4°C for 5 minutes, the supernatant was collected for analysis. Five proinflammatory cytokines (IL-1β, IL-6, MMP-1, TNF-α, and MMP-9) were analyzed in serum using a multi-analyte ELISArray kit (Sigma-Aldrich). A standard colorimetric sandwich ELISA method using highly specific antibodies based on inflammatory properties was used, and color development was recorded at 450 nm and 570 nm using a Labsystems iEMS Reader MF microtiter.

[0091] Enzymes containing gelatinase activity were detected using the SDS-PAGE substrate-embedded enzyme method. Mouse skin samples were homogenized with 0.01M CaCl2 and 1% protease inhibitor cocktail in 0.05M Tris-HCl buffer (pH 7.4). The homogenate was centrifuged at 4°C for 10 minutes. After two centrifugations, 30 μL of the resulting supernatant was mixed with 200 μL of 0.05M K2HPO4 buffer (pH 6.0) containing 0.0167% o-benzylamine hydrochloride and 0.05% hydrogen peroxide. The absorbance was measured at 450 nm (Asys Expert Plus, Biochrom). For experimental results, please refer to the results. Figure 1-Figure 5 . Figure 1-Figure 5 The effects of anti-photoaging lotion on inflammatory factors in photoaged skin of mice are shown respectively. Figure 1 Schematic diagram showing the effect of anti-photoaging lotion on inflammatory factor IL-1β in photoaged skin of mice; Figure 2 A schematic diagram showing the effect of the anti-photoaging lotion on the inflammatory factor IL-6 in photoaged skin of mice; Figure 3 A schematic diagram showing the effect of the anti-photoaging lotion on the inflammatory factor MMP-1 in photoaged skin of mice; Figure 4 A schematic diagram showing the effect of the anti-photoaging lotion on the inflammatory factor MMP-9 in photoaged skin of mice; Figure 5 A schematic diagram showing the effects of the anti-photoaging lotion on the inflammatory factor TNF-α in photoaged skin of mice. Figure 1-Figure 5 #p<0.01 compared with the normal group; *p<0.05 or **p<0.01 compared with the UVB irradiation group.

[0092] See also Figure 1-Figure 5 Compared with the normal group, the UVB irradiation group showed a significant increase in IL-1β, IL-6, MMP-1, TNF-α, and MMP-9 (p<0.05 or p<0.01). Applying the anti-photoaging lotion before irradiation significantly reduced IL-1β, IL-6, MMP-1, TNF-α, and MMP-9, which was significantly different from the UVB irradiation group, and had a better effect in preventing photodamage. Applying the anti-photoaging lotion after irradiation significantly reduced IL-1β, IL-6, MMP-1, TNF-α, and MMP-9, which was significantly different from the UVB irradiation group, and had a better effect in repairing photodamage.

[0093] Example 3 Effects of anti-photoaging lotion on skin edema in mice exposed to mild light irradiation.

[0094] The experimental grouping was the same as in Example 2. Mouse models that had not yet developed skin keratinization (small wrinkles appeared on the skin but no hardening or peeling of the skin) after one week of irradiation were selected and enrolled in each group, with 4 mice in each group.

[0095] Experimental Methods: The enrolled mice were irradiated with UVB at 1× MED intensity for one week, with irradiation every other day (three times per week). Grouping and pre- and post-irradiation treatments were the same as in Example 2. Twenty-four hours after the final irradiation, the mice were sacrificed, and skin tissue samples of equal size (1.5 cm in diameter) were obtained using a mold and weighed. The skin tissues were then dried at 105°C for 4 hours until the sample mass remained constant and then reweighed.

[0096] See Figure 6 , Figure 6 The skin weight test results of each experimental group are shown. It can be understood that the UVB irradiation group will cause skin edema, which in turn leads to a significant increase in skin water content.

[0097] Compared with the normal group, the skin water content of the UVB irradiation group increased significantly (p < 0.05), indicating that the UVB irradiation group in this embodiment will cause skin edema, which in turn leads to a significant increase in skin water content, indicating that the modeling is successful. Figure 6 The pre-treatment group) can reduce skin edema caused by UVB irradiation (p < 0.05). Applying anti-photoaging lotion after UVB irradiation (corresponding to Figure 6 treatment group), it can significantly reduce skin edema caused by UVB irradiation (p<0.05).

[0098] Therefore, this embodiment can significantly reduce skin edema caused by UVB and reduce the levels of aging markers, thereby significantly improving the skin photoaging phenomenon.

[0099] In summary, this example adds ergothioneine, thiotaurine, niacinamide, and resveratrol to naringenin to obtain a photoaging repair composition. The repair composition of this example has significant antioxidant, anti-inflammatory, and DNA protective effects, effectively inhibiting UVB-induced DNA damage, reducing the overexpression of inflammatory factors such as IL-1β, IL-6, MMP-1, TNF-α, and MMP-9, alleviating skin edema, and reducing the levels of aging markers, thereby significantly improving skin photoaging.

[0100] The above is a detailed introduction to the technical solutions provided in the embodiments of the present application. Specific examples are used herein to illustrate the principles and implementation methods of the embodiments of the present application. The description of the above embodiments is only applicable to help understand the principles of the embodiments of the present application. At the same time, for those skilled in the art, according to the embodiments of the present application, there may be changes in the specific implementation methods and application scope. In summary, the contents of this specification should not be understood as limiting the present application.

Claims

1. A composition for repairing skin photoaging, characterized in that: The repair composition comprises, by weight percentage: Naringenin: 1%~20%; Ergothioneine: 0.1%~10%; Thiotaurine: 0.5%~10%; Niacinamide: 1%~15%; Resveratrol: 1%~15%; Based on the total weight of the repair composition.

2. The skin photoaging repair composition according to claim 1, characterized in that The repair composition comprises: Naringenin: 5%~10%; Ergothioneine: 0.1%~2%; Thiotaurine: 0.5%~5%; Niacinamide: 1%~5%; Resveratrol: 1%~5%; Based on the total weight of the repair composition.

3. The skin photoaging repair composition according to claim 1 or 2, characterized in that The repair composition further comprises at least one cosmetic additive.

4. The skin photoaging repair composition according to claim 3, characterized in that The cosmetic additive is selected from at least one of betaine and butylene glycol.

5. A method for preparing the skin photoaging repair composition according to any one of claims 1 to 4, characterized in that: The preparation method comprises: Weigh each component in proportion, and stir the components evenly at 40-60° C. to obtain a repair composition.

6. Use of the skin photoaging repair composition according to any one of claims 1 to 4 in the preparation of a product for improving and / or alleviating UVB-induced skin photoaging symptoms.

7. The use according to claim 6, characterized in that The skin photoaging symptoms are at least one of sunburn, erythema, peeling, pigmentation, dry skin, loose skin or rough skin.

8. Use of a combination of naringenin, ergothioneine, thiotaurine, niacinamide and resveratrol in preparing a composition for repairing skin photoaging.

9. The use according to claim 8, characterized in that In the repair composition, the content of naringenin is 1% to 20%, the content of ergothioneine is 0.1% to 10%, the content of thiotaurine is 0.5% to 10%, the content of niacinamide is 1% to 15%, and the content of resveratrol is 1% to 15%, based on the total weight of the repair composition.

10. The use according to claim 8 or 9, characterized in that In the repair composition, the content of naringenin is 5% to 10%, the content of ergothioneine is 0.1% to 2%, the content of thiotaurine is 0.5% to 5%, the content of niacinamide is 1% to 5%, and the content of resveratrol is 1% to 5%, based on the total weight of the repair composition.

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