Pickering emulsion composition for protecting vesicular structures, and method for preparing and using the same

Abstract

The application discloses a Pickering emulsion composition for protecting vesicle structures and a preparation method and application thereof, and belongs to the technical field of plant preparations, and solves the problem of poor stability of plant vesicles in application in the prior art. The Pickering composition comprises the following components: a humectant 6-10%, an emollient 3-10%, xanthan gum 0.1-0.3%, microcrystalline cellulose 2-4%, an anti-inflammatory active component 8-13%, and the balance is water; wherein, the anti-inflammatory active component comprises rose exosome 3-5%, grape fruit exosome 2-4% and spone exosome 1-4% according to the total mass of the Pickering composition. The Pickering emulsion is used to stabilize the emulsion system in the form of solid particles, the plant vesicle structure is protected, and double regulation of inflammation and aging is realized.

Description

Technical Field

[0001] This invention relates to the field of plant preparation technology, and more specifically to a Pickering emulsion composition for protecting vesicle structures, its preparation method, and its application. Background Technology

[0002] In existing technologies, treatments for inflammatory senescence mostly focus on a single target (such as inhibiting specific inflammatory factors), lacking a solution to regulate the synergistic effect of chronic inflammation and cellular senescence from the source.

[0003] Plant-derived vesicles (PDVs) are a type of bioactive nanoparticle discovered in recent years, possessing potential anti-inflammatory, antioxidant, and anti-aging effects. However, plant vesicles exhibit poor stability, are easily affected by external environmental factors during extraction and storage, and are prone to degradation, leading to structural damage and loss of function, thus limiting their applications. Furthermore, in existing technologies, the emulsifying components in cosmetics are all emulsifiers, but existing emulsifiers have certain skin irritation properties.

[0004] Therefore, it is of great significance to develop a composition that can protect plant vesicle structure and enhance its anti-inflammatory and anti-aging functions. Summary of the Invention

[0005] This invention provides a Pickering emulsion composition for protecting vesicle structures, its preparation method, and its application, in order to solve the problem of poor stability of plant vesicles in applications in the prior art.

[0006] In a first aspect, the present invention provides a Pickering composition comprising, by weight, the following components: 6%–10% humectant, 3%–10% emollient, 0.1%–0.3% xanthan gum, 2%–4% microcrystalline cellulose, 8%–13% anti-inflammatory active ingredient, and the balance being water; wherein, by weight of the total amount of the Pickering composition, the anti-inflammatory active ingredient comprises 3%–5% rose exosomes, 2%–4% grape fruit exosomes, and 1%–4% purslane exosomes.

[0007] As one possible implementation, the composition comprises, by weight, the following components: 8% moisturizer, 5% emollient, 0.3% xanthan gum, 4% microcrystalline cellulose, 13% anti-inflammatory active ingredient, and the balance being water; wherein, by weight of the total amount of the Pickering composition, the anti-inflammatory active ingredient comprises 5% rose exosomes, 4% grape fruit exosomes, and 4% purslane exosomes.

[0008] As one possible implementation, the moisturizer is any one or a combination of several of butylene glycol, sodium hyaluronate, glycerin, and glyceryl polyether-26; and / or, the emollient is any one or a combination of several of diethylhexyl carbonate, polydimethylsiloxane, caprylic / capric triglyceride, cetyl ethylhexanoate, meadowfoam seed oil, and vitamin E.

[0009] As one possible implementation, the particle size of its anti-inflammatory active component is 100–200 nm.

[0010] As one possible implementation, it can withstand temperatures of -18 to 50°C, light exposure of 0 to 4500 lx, rotation speed of 0 to 4000 rpm, and storage time of at least 3 months.

[0011] In a second aspect, the present invention provides a method for preparing the Pickering composition according to any possible implementation of the first aspect, comprising the steps of: mixing the moisturizer, the microcrystalline cellulose, the xanthan gum and an appropriate amount of purified water, heating and stirring to dissolve until transparent, and maintaining the temperature to obtain an aqueous phase mixture; heating the emollient under stirring conditions and stirring until all the oil is dissolved to obtain an oil phase; adding the oil phase to the aqueous phase mixture, performing homogenization and stirring, and cooling to 40-45°C to obtain a homogeneous mixture; mixing the anti-inflammatory active component evenly and then adding it to the homogeneous mixture, stirring, to obtain the Pickering composition.

[0012] As one possible implementation, during the preparation of the aqueous phase mixture, the temperature is raised to 75°C and held for 25 minutes; and / or, during the preparation of the oil phase, the emollient is heated to 70-80°C under stirring conditions, with the stirring rate being such that the liquid surface sways slightly; and / or, when the oil phase is added to the aqueous phase mixture, the homogenization stirring conditions are 70-75°C, 50Hz, and 10 minutes; and / or, when the anti-inflammatory active component is mixed evenly and then added to the homogenized mixture, the stirring rate is such that the liquid surface sways slightly, and the stirring time is 35 minutes.

[0013] Thirdly, the present invention provides the use of the Pickering composition described in any possible implementation of the first aspect or the Pickering composition prepared by the preparation method described in any possible implementation of the second aspect in the preparation of skin care products.

[0014] As one possible implementation, the skincare product is used for anti-inflammatory and anti-aging purposes.

[0015] This invention achieves dual regulation of inflammatory senescence by using Pickering emulsion to stabilize the emulsion system with solid particles, protecting the plant vesicle structure.

[0016] This invention utilizes Pickering emulsification technology to protect the vesicle structure. Micellar particles adsorb onto the surface of plant vesicles, forming a physical barrier that effectively protects the vesicles from external environmental factors, preventing rupture and aggregation, and preserving the integrity of the vesicle structure. It also improves the stability of the composition. The addition of xanthan gum promotes the formation of the Pickering structure, enhancing its interaction with the plant vesicles and resulting in excellent stability of the Pickering composition. The loss of active ingredients from plant vesicles is reduced, enhancing anti-inflammatory and anti-aging effects. The antioxidant and anti-inflammatory active ingredients encapsulated within the plant vesicles are stably preserved under the protection of starch granules and released slowly. These active ingredients effectively scavenge free radicals in the skin, inhibit the production of inflammatory factors, promote skin cell metabolism, and enhance the skin's self-repair ability, thereby significantly enhancing anti-inflammatory and anti-aging effects.

[0017] Microcrystalline cellulose extracted from Sargassum fusiforme can form an emulsifier-free composition. The emulsifier-free, naturally derived microcrystalline cellulose particles adsorb onto the surface of plant vesicles, forming a physical barrier that protects the plant exosome vesicles and keeps them stable in the formulation. This not only reduces the irritation of emulsifiers to the skin but also repairs the skin barrier. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a SEM image of composition A provided in an embodiment of the present invention.

[0020] Figure 2 This is a SEM image of composition D provided in an embodiment of the present invention.

[0021] Figure 3 This is a SEM image of composition F provided in an embodiment of the present invention.

[0022] Figure 4 The image shows the microstructure of composition A provided in this embodiment of the invention under a confocal microscope.

[0023] Figure 5 Microscopic structure diagram (10×) of composition A provided in the embodiments of the present invention under an optical microscope.

[0024] Figure 6 Microscopic structure diagram (4×) of composition A provided in the embodiments of the present invention under an optical microscope.

[0025] Figure 7 The image shows the TEM structure of composition A provided in an embodiment of the present invention.

[0026] Figure 8 The image shows the microstructure of composition F provided in the embodiments of the present invention under an optical microscope.

[0027] Figure 9 The image shows the microstructure of composition G provided in the embodiments of the present invention under an optical microscope.

[0028] Figure 10 The bilayer membrane vesicle structure of composition A provided in the embodiments of the present invention is obtained by SEM after acceleration at 45°C for 3M.

[0029] Figure 11 The results show the detection of changes in filaggrin (FLG) content in compositions A and E provided in the embodiments of the present invention.

[0030] Figure 12 The results show the detection of changes in lobe protein (LOR) content in compositions A and E provided in the embodiments of the present invention.

[0031] Figure 13 The results of the detection of changes in the content of pro-inflammatory factor (IL-1α) in compositions A and E provided in the embodiments of the present invention.

[0032] Figure 14 The results show the detection of changes in the content of inflammatory mediators (PGE2) in compositions A and E provided in the embodiments of the present invention. Detailed Implementation

[0033] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0034] To address the problem of poor stability of plant vesicles in applications in existing technologies, this invention provides a preparation experiment of a Pickering emulsion composition for protecting vesicle structures, a physical property testing experiment of the composition, and an application experiment of the composition.

[0035] This invention relates to a composition comprising naturally derived microcrystalline cellulose and xanthan gum, which interact in solution via hydrogen bonding and electrostatic forces to form a composite three-dimensional network structure. The high viscosity of xanthan gum and the crystallinity of microcrystalline cellulose work together to enhance the mechanical strength and stability of the system, forming a Pickering emulsion. This allows droplets to share colloidal particles, forming a three-dimensional network gel structure that encapsulates anti-inflammatory and anti-aging plant exosomes within this network. The hydrogel's three-dimensional network structure restricts the movement of exosome vesicles, reducing collisions and aggregation. The hydrogel also provides a humid environment for the exosome vesicles, preventing rupture under dry conditions. This improves the long-term stability and efficacy of the composition, protecting the vesicle structure, maximizing the anti-inflammatory and antioxidant effects of the plant exosomes, and enhancing the overall stability of the composition. A physical barrier is formed to protect the plant exosome vesicles and ensure their stable presence in the formulation.

[0036] The technical solution of the present invention will be further described below with reference to specific embodiments.

[0037] Example 1

[0038] This embodiment provides an experimental preparation of a Pickering composition.

[0039] The composition includes: 8% humectant (3% butylene glycol, 0.1% sodium hyaluronate, 4.9% glycerin), 5% emollient (2.5% diethylhexyl carbonate, 2.5% polydimethylsiloxane), 0.3% xanthan gum, 4% microcrystalline cellulose, 13% anti-inflammatory active ingredient (5% rose exosomes, 4% grape fruit exosomes, 4% purslane exosomes), and the balance being water. A moisturizing agent, microcrystalline cellulose, xanthan gum, and an appropriate amount of purified water were mixed, heated to 75°C, and stirred until dissolved and transparent. The mixture was kept at this temperature for 25 minutes to obtain an aqueous phase mixture. The emollient was heated to 70–80°C under stirring, with the stirring speed adjusted to allow slight sloshing of the liquid surface, until all the oils were dissolved to obtain an oil phase. The oil phase was added to the aqueous phase mixture and homogenized at 70–75°C for 10 minutes at 50 Hz. The mixture was then cooled to 40–45°C to obtain a homogeneous mixture. The anti-inflammatory active ingredients were mixed and added to the homogeneous mixture. The stirring speed was adjusted to allow slight sloshing of the liquid surface, and the mixture was stirred for 35 minutes to obtain composition A.

[0040] The composition includes: 7% humectant (3.5% butylene glycol, 0.05% sodium hyaluronate, 3.45% glycerin), 3% emollient (1.5% diethylhexyl carbonate, 1.5% caprylic / capric triglyceride), 0.2% xanthan gum, 4% microcrystalline cellulose, 8% anti-inflammatory active ingredients (3% rose exosomes, 3% grape fruit exosomes, 2% purslane exosomes), and the balance being water. A moisturizing agent, microcrystalline cellulose, xanthan gum, and an appropriate amount of purified water were mixed, heated to 65°C, and stirred until dissolved and transparent. The mixture was kept at this temperature for 25 minutes to obtain an aqueous phase mixture. The emollient was heated to 70–80°C under stirring, with the stirring speed adjusted to allow slight sloshing of the liquid surface, until all the oils were dissolved to obtain an oil phase. The oil phase was added to the aqueous phase mixture and homogenized at 65–70°C for 8 minutes at 70 Hz. The mixture was then cooled to 40–45°C to obtain a homogeneous mixture. The anti-inflammatory active ingredients were mixed and added to the homogeneous mixture. The stirring speed was adjusted to allow slight sloshing of the liquid surface, and the mixture was stirred for 35 minutes to obtain composition B.

[0041] The composition includes: 10% moisturizer (glyceryl polyether-261.5%, sodium hyaluronate 0.2%, glycerin 3.5%, butylene glycol 4.8%), 10% emollient (cetyl ethylhexanoate 3%, meadowfoam seed oil 2%, vitamin E 0.3%, polydimethylsiloxane 4.7%), 0.25% xanthan gum, 3% microcrystalline cellulose, 9% anti-inflammatory active ingredients (rose exosomes 4%, grape fruit exosomes 4%, purslane exosomes 1%), and the balance being water. A moisturizing agent, microcrystalline cellulose, xanthan gum, and an appropriate amount of purified water were mixed, heated to 70°C, and stirred until dissolved and transparent. The mixture was kept at this temperature for 25 minutes to obtain an aqueous phase mixture. The emollient was heated to 70–80°C under stirring, with the stirring speed adjusted to allow slight sloshing of the liquid surface, until all the oils were dissolved to obtain an oil phase. The oil phase was added to the aqueous phase mixture and homogenized at 60–65°C for 10 minutes at 70 Hz. The mixture was then cooled to 40–45°C to obtain a homogeneous mixture. The anti-inflammatory active ingredients were mixed and added to the homogeneous mixture. The stirring speed was adjusted to allow slight sloshing of the liquid surface, and the mixture was stirred for 35 minutes to obtain composition C.

[0042] The composition consists of 6% humectant (3% butylene glycol, 0.15% sodium hyaluronate, 2.85% glycerin), 3% microcrystalline cellulose, 0.1% xanthan gum, 2% microcrystalline cellulose, 8% anti-inflammatory active ingredients (3% rose exosomes, 2% grape fruit exosomes, 3% purslane exosomes), and the remainder is water. The humectant, microcrystalline cellulose, xanthan gum, and an appropriate amount of purified water are mixed, heated to 65°C, stirred until dissolved and transparent, and kept at this temperature for 25 minutes to obtain an aqueous phase mixture. This aqueous phase mixture is then homogenized at 55–60°C with stirring at 70 Hz for 10 minutes, and then cooled to 40–45°C to obtain a homogeneous mixture. The anti-inflammatory active ingredients are mixed and added to the homogeneous mixture, the stirring speed is adjusted to allow slight surface agitation, and the mixture is stirred for 35 minutes to obtain composition D.

[0043] The preparation process differs from that of composition A in that rose exosomes, grape fruit exosomes, and purslane exosomes are not added, while all other processes are the same, resulting in composition E.

[0044] The difference between the preparation process of composition A and composition F is that microcrystalline cellulose is not added, while all other processes are the same, resulting in composition F.

[0045] The preparation process differs from that of composition A in that xanthan gum is not added, while all other steps are the same, resulting in composition G.

[0046] The difference between the preparation process of composition H and composition A is that the same mass of titanium dioxide is used to replace microcrystalline cellulose, while the rest are the same, to obtain composition H.

[0047] Example 2

[0048] This embodiment provides a performance testing experiment for a Pickering composition.

[0049] 10g of the sample to be tested was kept at 40℃ for 1 hour, and then centrifuged at speeds of 2000rpm, 3000rpm and 4000rpm respectively. In this example, the compositions A to H obtained in Example 1 were used as the samples to be tested, and the results are shown in Table 1.

[0050] Table 1 Centrifugation test results

[0051] sample Discharge status 2000rpm 3000rpm 4000rpm Composition A normal normal normal normal Composition B normal normal normal normal Composition C normal normal normal normal Composition D normal normal normal normal Composition E normal normal normal normal Composition F normal Oily Breast breaking Breast breaking Composition G normal Oily Breast breaking Breast breaking Composition H normal Oily Breast breaking Breast breaking

[0052] Table 1 shows that compositions A-E exhibit good stability, tolerating speeds of 4000 rpm; compositions F-H show poor stability, failing to tolerate speeds of 2000 rpm. Comparing compositions A and F-G, it is evident that the combination of xanthan gum and microcrystalline cellulose with other components significantly improves the stability of the compositions. This is because the combination of microcrystalline cellulose and xanthan gum, when prepared into a Pickering emulsion, improves the stability of the Pickering micelle structure formed by the emulsion, thereby enhancing the stability of plant exosome vesicles within the three-dimensional network structure formed by xanthan gum and Pickering, preserving the effective concentration in the formulation, and better exerting its anti-inflammatory, soothing, and antioxidant effects. Comparing compositions A and H, it is clear that in the compositions provided by this invention, microcrystalline cellulose exhibits superior emulsifying stability compared to titanium dioxide.

[0053] 100g of the test sample was placed in different environments [Influencing factor experiment: high temperature (50℃), light (illuminance: 4500lx), cold resistance (-18℃); accelerated experiment: 45℃; long-term experiment: room temperature (25℃)] to test its stability. In this example, the compositions A to H obtained in Example 1 were used as test samples and the results are shown in Table 2.

[0054] Table 2. Results of extreme environmental stability tests on samples (pH / stratification)

[0055]

[0056]

[0057] Table 2 shows that compositions A-E exhibit good stability and can withstand extreme environments under different conditions, while compositions F-H show poor stability, exhibiting varying degrees of stratification under different extreme conditions. Comparing compositions A and F-G, it is evident that the combination of xanthan gum and microcrystalline cellulose with other components significantly improves the stability of the compositions. This is because the combination of microcrystalline cellulose and xanthan gum, when prepared into a Pickering emulsion, improves the stability of the Pickering micelle structure formed by the Pickering emulsion, thereby enhancing the stability of plant exosome vesicles within the three-dimensional network structure formed by xanthan gum and Pickering, preserving the effective concentration in the formulation, and better exerting its anti-inflammatory, soothing, and antioxidant effects. Comparing compositions A and H, it is clear that in the compositions provided by this invention, microcrystalline cellulose exhibits better emulsifying stabilizing effects than titanium dioxide.

[0058] After storing 100g of the test sample at temperatures of -18℃, 4℃, 45℃, and 50℃ for three months, its stability was tested at different speeds. In this example, the compositions A to H obtained in Example 1 were used as test samples, and the results are shown in Table 3.

[0059] Table 3 Sample Centrifugation Test

[0060] sample Material state 2000rpm 3000rpm 4000rpm Composition A normal normal normal normal Composition B normal normal normal normal Composition C normal normal normal normal Composition D normal normal normal normal Composition E normal normal normal normal Composition F Slight stratification Oily Oil production and sedimentation Oil production and sedimentation Composition G Slight stratification Oily Oil production and sedimentation Oil production and sedimentation Composition H Slight stratification Oily Oil production and sedimentation Oil production and sedimentation

[0061] Table 3 shows that compositions A-E exhibit good stability, withstanding centrifugation at 4000 rpm after 3 months of storage at different temperatures. Compositions F-H show poor stability, exhibiting varying degrees of oil exudation or stratification at different speeds after 3 months of storage under different temperature conditions. A comparison of compositions A and F-G reveals that the combination of xanthan gum and microcrystalline cellulose with other components significantly improves the stability of the compositions. This is because the combination of microcrystalline cellulose and xanthan gum, when prepared into a Pickering emulsion, improves the stability of the Pickering micelle structure formed by the emulsion, thereby enhancing the stability of plant exosome vesicles within the three-dimensional network structure formed by xanthan gum and Pickering, preserving the effective concentration in the formulation, and better exerting its anti-inflammatory, soothing, and antioxidant effects. A comparison of compositions A and H shows that, in the compositions provided by this invention, microcrystalline cellulose exhibits better emulsification stabilization than titanium dioxide.

[0062] Example 3

[0063] This embodiment provides an experiment to test the stability of the vesicle structure of a Pickering composition.

[0064] Compositions A, D, and F were imaged using a transmission electron microscope and a scanning electron microscope (SEM) with negative staining technique, as shown below. Figures 1-3 The results are shown. It is evident that compositions A and D utilize a three-dimensional network micelle particle structure that protects the vesicle structure. Under negative staining, numerous regular, uniform, double-membrane vesicle structures with a particle size of 100–200 nm can be observed, representing the normal vesicle structure of plant exudates. Composition F does not contain a microcrystalline cellulose Pickering emulsifier; under transmission electron microscopy, the vesicle structure is somewhat damaged, irregular, and incomplete, with some contents leaking out.

[0065] The microstructure characterization of composition A under a confocal microscope is shown in the figure below. Figure 4 As shown, the microstructure of composition A under an optical microscope is as follows. Figure 5 (10×) and Figure 6 As shown in (4×), it can be seen that under a confocal microscope, the composition prepared by combining microcrystalline cellulose and xanthan gum shows a large number of spherical solid particles adsorbed at the oil-water interface, forming a stable three-dimensional network interfacial film and a stable emulsion system; a large number of spherical micelle particles are dispersed in the emulsion interface, and the system is stable.

[0066] The structural characterization image of composition A taken under a scanning electron microscope (TEM) is shown below. Figure 7As shown, a large number of solid particles are adsorbed on the interface surface, forming a stable spherical particle structure.

[0067] The microstructures of compositions F and G under a microscope are shown in the following figures. Figure 8 and Figure 9 As shown, the composition without microcrystalline cellulose does not contain solid spherical particles under a microscope; instead, a large number of droplets are dispersed at the water-oil interface, forming a relatively stable system. It is evident that xanthan gum or microcrystalline cellulose alone cannot synergistically form a stable three-dimensional network structure through the hydrogen bonding between them, thus failing to form a Pickering structure and protect the plant exosome vesicle structure, which is detrimental to its stability. Furthermore, the plant exosome vesicle structure of composition G is destroyed after 2 minutes, with the double phospholipid membrane ruptured, thus preventing it from exerting its anti-inflammatory, soothing, and antioxidant effects.

[0068] SEM images of composition A after accelerated 3M under different conditions are shown below. Figure 10 As shown, the double-membrane vesicle structure is regular and uniform with a particle size of 100-200 nm. It can be seen that the three-dimensional network gel structure formed by composition A can protect the long-term storage of plant exosome vesicle structures, prevent them from rupture, aggregation, and sedimentation during the acceleration process, and protect the trace amino acids, proteins, and nucleic acid molecules in the exosome vesicles from degradation, thus exerting anti-inflammatory, antioxidant, and soothing effects.

[0069] Example 4

[0070] This embodiment provides an experimental study on the application effect of a Pickering composition.

[0071] This embodiment uses an SLS-stimulated 3D epidermal skin model. An in vitro skin injury model was constructed. The repair efficacy of the test samples was evaluated by detecting changes in tissue vitality, tissue morphology, lobe rhinol (LOR) content, and filaggrin (FLG) content in the skin model after the application of the composition. The soothing efficacy of the test samples was evaluated by detecting changes in pro-inflammatory factor (IL-1α) content and inflammatory mediator (PGE2) content.

[0072] Sodium lauryl sulfate (SLS), an anionic surfactant, is amphiphilic (both hydrophilic and lipophilic). At higher concentrations, it can damage the skin barrier, particularly the lipid components and cell membranes, further damaging living cell membranes and releasing large amounts of IL-1α. IL-1α then activates NFκB, releasing even more pro-inflammatory factors, thus amplifying the inflammatory cascade. Prostaglandin E2 (PGE2), a metabolite of arachidonic acid, primarily induces inflammatory responses. The decrease in PGE2 levels after treatment with the analyte indicates a certain soothing effect. Therefore, a soothing effect can be achieved by inhibiting the pro-inflammatory factor IL-1α and the inflammatory mediator PGE2. Skin tissue, when exposed to external stimuli, suffers damage to the skin barrier, further damaging living cells and leading to decreased tissue activity. Therefore, changes in tissue activity are a key indicator for evaluating the degree of damage and its alleviation. Tissue morphology, analyzed through HE staining, reveals changes in the skin barrier under different treatment conditions. For example, SLS damage weakens the skin barrier, reducing the thickness of the living cell layer, while treatment with active ingredients can improve this damage. LOR (Leukocorticoid Olefin) is a key component in the protein membrane CE (Cellular Activated Collagen) assembly process, accounting for approximately 80% of CE content, and plays a reinforcing role in the skin barrier. Its decreased content is a major factor in weakened skin barrier function. FLG (Firming Glycerin) is also a key component in CE assembly. Besides being a structural component of the skin barrier, FLG can be hydrolyzed by Caspase-14 to form natural moisturizing factors, thus also playing a moisturizing role. Therefore, by detecting changes in LOR, FLG, TNF-α, and IL-8 levels in a skin model after sample treatment, the repair and anti-inflammatory effects of the composition can be evaluated.

[0073] In this embodiment, composition A and composition E were used as analytes to detect barrier-related proteins (FLG & LOR) and inflammatory factors (IL-1α & PGE2), respectively, and the results were as follows: Figures 11-14 The results are shown, where BC represents the blank control and NC represents the negative control.

[0074] Depend on Figure 11 It can be seen that, compared with group BC, the FLG protein content in group NC decreased significantly, indicating that the stimulation conditions in this test were effective. Compared with group NC, the FLG protein content of composition E increased significantly, and the FLG protein content of composition A also increased significantly, with composition A showing a greater increase than composition E, proving that composition A has a more significant skin repair effect.

[0075] Depend on Figure 12It can be seen that, compared with group BC, the LOR protein content in group NC decreased significantly, indicating that the stimulation conditions in this test were effective. Compared with group NC, the LOR protein content of composition E increased significantly, and the LOR protein content of composition A also increased significantly, with composition A showing a greater increase than composition E, proving that composition A has a more significant skin repair effect.

[0076] Depend on Figure 13 It was found that the TNF-α secretion level in the NC group was significantly higher than that in the BC group, indicating that the stimulation conditions in this test were effective. Compared with the NC group, composition E could inhibit the secretion of TNF-α induced by SLS stimulation, while composition A had a significant inhibitory effect on TNF-α secretion, and the inhibitory effect of composition A was higher than that of composition E, thus having a more significant soothing and anti-inflammatory effect on the skin.

[0077] Depend on Figure 14 It was found that the IL-8 secretion level in the NC group was significantly higher than that in the BC group, indicating that the stimulation conditions in this test were effective. Compared with the NC group, composition E could inhibit the secretion of IL-8 induced by SLS stimulation, while composition A had a significant inhibitory effect on IL-8 secretion, and the inhibitory effect of composition A was higher than that of composition E, thus having a more significant soothing and anti-inflammatory effect on the skin.

[0078] Although preferred embodiments of the invention have been described, those skilled in the art, upon learning the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of the invention.

[0079] Obviously, those skilled in the art can make various modifications and variations to this invention without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this invention and their equivalents, this invention also intends to include these modifications and variations.

Claims

1. A Pickering composition, characterized in that, It includes the following components by mass percentage: Moisturizer 6%~10%, emollient 3%~10%, xanthan gum 0.1%~0.3%, microcrystalline cellulose 2%~4%, anti-inflammatory active ingredient 8%~13%, balance water; The anti-inflammatory active components, based on the total mass of the Pickering composition, include 3% to 5% rose exosomes, 2% to 4% grape fruit exosomes, and 1% to 4% purslane exosomes.

2. The Pickering composition according to claim 1, characterized in that, It includes the following components by mass percentage: Moisturizer 8%, emollient 5%, xanthan gum 0.3%, microcrystalline cellulose 4%, anti-inflammatory active ingredient 13%, balance water; The anti-inflammatory active components, based on the total mass of the Pickering composition, include 5% rose exosomes, 4% grape fruit exosomes, and 4% purslane exosomes.

3. The Pickering composition according to claim 1, characterized in that, The moisturizer is any one or a combination of several of butylene glycol, sodium hyaluronate, glycerin, and glyceryl polyether-26; And / or, the emollient is any one or a combination of several of the following: diethylhexyl carbonate, polydimethylsiloxane, caprylic / capric triglyceride, cetyl ethylhexanoate, meadowfoam seed oil, and vitamin E.

4. The Pickering composition according to claim 1, characterized in that, Its anti-inflammatory active components have a particle size of 100~200 nm.

5. The Pickering composition according to claim 1, characterized in that, Its temperature tolerance is -18~50℃, its light tolerance is 0~4500 lx, its rotation speed tolerance is 0~4000 rpm, and its storage tolerance is at least 3 months.

6. A method for preparing the Pickering composition according to any one of claims 1 to 5, characterized in that, Including the following steps: The humectant, the microcrystalline cellulose, the xanthan gum, and an appropriate amount of purified water are mixed, heated and stirred until dissolved and transparent, and kept at the temperature to obtain an aqueous mixture. The emollient is heated under stirring and stirred until all the oil is dissolved to obtain the oil phase. The oil phase is added to the aqueous phase mixture, and homogenization and stirring are performed. The mixture is then cooled to 40-45°C to obtain a homogeneous mixture. The anti-inflammatory active components are mixed evenly and then added to the homogenized mixture. The mixture is stirred to obtain the Pickering composition.

7. The preparation method according to claim 6, characterized in that, During the preparation of the aqueous mixture, the temperature is raised to 65~75℃ and held for 25 minutes; And / or, during the preparation of the oil phase, the emollient is heated to 70-80°C under stirring conditions, and the stirring rate is such that the liquid surface sways slightly. And / or, the oil phase is added to the aqueous phase mixture, and the homogenization stirring conditions are 60~75℃, 50Hz, and 10 min. And / or, after the anti-inflammatory active components are mixed evenly, they are added to the homogeneous mixture, and the stirring rate is such that the liquid surface sways slightly, and the stirring time is 35 minutes.

8. The use of the Pickering composition according to any one of claims 1 to 5 or the Pickering composition prepared by the preparation method according to any one of claims 6 to 7 in the preparation of skin care products.

9. The application according to claim 8, characterized in that, The skincare products are used for anti-inflammatory and anti-aging purposes.

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