Traditional Chinese medicine moxa stick for reinforcing kidney and strengthening primordial qi and preparation method of traditional Chinese medicine moxa stick

Abstract

The invention belongs to the technical field of moxa stick processing, and particularly relates to a traditional Chinese medicine moxa stick for reinforcing kidney and consolidating primordial qi and a preparation method thereof. The preparation method of the traditional Chinese medicine moxa stick comprises the following steps: (1) dispersing moxa in water, performing ultrasonic treatment, filtering, and drying for later use; (2) weighing traditional Chinese medicine components according to the weight, mixing, crushing and sieving to obtain traditional Chinese medicine powder; (3) mixing the modified pullulan with paper mulberry bark powder to obtain an adhesive; (4) adding an adhesive into water, adding the traditional Chinese medicine powder, and uniformly mixing to obtain a mixture; and (5) adding the moxa into the mixture, fully and uniformly mixing, pressing, and drying to obtain the moxa stick. When the traditional Chinese medicine moxa stick obtained through the preparation method is burnt, medicine powder can be fixedly attached to moxa, and the defects that medicine powder is prone to falling off during burning, and effective components of traditional Chinese medicine are unevenly released are overcome. The process is simple in step and mild in condition, and the use safety of the moxa stick cannot be affected during sufficient combustion.

Description

Technical Field

[0001] The invention belongs to the technical field of moxa stick processing, and particularly relates to a traditional Chinese medicine moxa stick for strengthening the kidney and nourishing essence, and a preparation method thereof. Background Art

[0002] Today's fast-paced lifestyle places immense physical and mental stress on most people, leading to symptoms such as insomnia, fatigue, irritability, and irregular sleep patterns, all of which can contribute to kidney deficiency. Moxibustion is an excellent treatment for kidney deficiency. Mugwort is a naturally yang plant. Moxibustion warms the body, replenishing yang energy and expelling cold. This ancient Chinese medicine natural health therapy is side-effect-free and simple to perform.

[0003] Traditional moxibustion mainly relies on the warming effect of pure moxa. Although it has the effects of warming the meridians and dredge the collaterals, and replenishing the middle and replenishing qi, its conditioning effect on specific symptoms such as kidney yang deficiency is limited. Existing moxibustion all uses pure moxa sticks for health care. There are also Chinese herbal moxa sticks, but the existing Chinese herbal moxa sticks are not very targeted, and the selected Chinese herbal medicines are expensive and costly, which cannot meet the needs of ordinary families for disease prevention and treatment. Moxa is obtained by repeatedly beating, crushing, and screening out impurities and dust from the leaves of the Asteraceae plant. It is soft like velvet, flammable but not flame-proof, and has a fragrant smell. Moxa is the raw material for making moxa sticks and is also the main material used in moxibustion. It has the effects of dredge the meridians and activate the collaterals, warm the meridians and stop bleeding, dispel cold and relieve pain, and maintain health. At present, most of the Chinese herbal moxa sticks on the market are mixed with moxa and other Chinese medicinal materials. When the moxa sticks are burned, the effective ingredients in the Chinese medicinal materials can evaporate and enter the lesions in the body through skin absorption. During moxibustion, the non-flammable Chinese medicinal materials in the moxa sticks cannot be fully burned, resulting in poor moxibustion effect and producing a large amount of irritating smoke, affecting the experience; the medicinal powder is easy to fall off during burning, resulting in uneven release of effective ingredients and reduced efficacy; another part of the moxa sticks, although it can be fully burned, the ashes after burning are easy to fall off, and the fallen ashes can easily burn the skin, and there are many defects in use.

[0004] Based on the above purpose, the present invention provides a traditional Chinese medicine moxa stick for strengthening the kidney and nourishing the essence and a preparation method thereof. Summary of the Invention

[0005] The first purpose of the present invention is to provide a method for preparing a traditional Chinese medicine moxa stick for strengthening the kidney and nourishing the essence.

[0006] The second object of the present invention is to provide a traditional Chinese medicine moxa stick for strengthening the kidney and nourishing the essence.

[0007] In order to achieve the above object, the technical solution adopted by the present invention is:

[0008] A method for preparing a traditional Chinese medicine moxa stick for strengthening the kidney and nourishing the essence comprises the following steps:

[0009] (1) Disperse moxa in water, treat with ultrasonic wave, filter, dry and set aside;

[0010] (2) Mixing the Chinese medicinal ingredients of Cistanche deserticola, Epimedium, Astragalus, Chuanxiong, and Angelica sinensis, and crushing and sieving to obtain Chinese medicinal powder;

[0011] (3) mixing the modified pullulan and paper mulberry bark powder in a mass ratio of 1:(1-3) to obtain an adhesive;

[0012] (4) adding deionized water to the adhesive obtained in step (3), and then adding the Chinese medicinal powder obtained in step (2) and mixing them uniformly to obtain a mixture;

[0013] (5) adding moxa to the mixture obtained in step (4), mixing thoroughly and evenly, and then pressing and drying to obtain moxa sticks.

[0014] Furthermore, the preparation process of the modified pullulan is as follows: after mixing a pullulan aqueous solution with dilute sulfuric acid, a sodium periodate aqueous solution is added dropwise, stirred in the dark, and the sodium periodate is removed and then freeze-dried to obtain oxidized pullulan; the oxidized pullulan is dissolved in anhydrous ethanol, and theanine is added, mixed and reacted to obtain modified pullulan.

[0015] Furthermore, the concentration of dilute sulfuric acid is 0.08-0.12 mol / L; the molar ratio of pullulan to sodium periodate is 1:(1-2); the light-proof stirring time is 4-6 hours; the freeze-drying time is 48-72 hours; the molar ratio of oxidized pullulan to theanine is 1:(1.1-1.5); the reaction temperature after adding theanine and mixing is 60-80°C, and the reaction time is 2-4 hours.

[0016] Furthermore, the frequency of the ultrasonic treatment in step (1) is 20-50 KHZ, the power is 400-600 W, and the time is 1-2 hours.

[0017] Furthermore, the weight proportions of the Chinese medicinal ingredients in step (2) are: 8-12 parts of Cistanche deserticola, 5-10 parts of Epimedium, 5-10 parts of Astragalus, 3-5 parts of Chuanxiong, and 1-4 parts of Angelica sinensis.

[0018] Furthermore, in the step (3), the mass ratio of the modified pullulan to the paper mulberry bark powder is 1:(1-3).

[0019] Furthermore, in step (4), the volume ratio of the adhesive to the deionized water is 1:(1-3).

[0020] Furthermore, the drying in step (5) is performed under normal pressure at a temperature of 60-80°C.

[0021] A traditional Chinese medicine moxa stick for strengthening the kidney and nourishing the essence is prepared according to the above preparation method.

[0022] Compared with the prior art, the beneficial effects of the present invention are mainly:

[0023] The invention uses moxa as raw material, adds traditional Chinese medicine with kidney-strengthening and vital essence-nourishing properties as auxiliary materials, and uses modified pullulan and paper mulberry bark powder as adhesives. The resulting traditional Chinese medicine moxa stick can firmly adhere the powder to the moxa, solving the problems of powder easily falling off during combustion and uneven release of the effective ingredients of the traditional Chinese medicine. Furthermore, the process is simple, the conditions are mild, and sufficient combustion does not affect the safety of the moxa stick. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a morphological diagram of fibroblasts cultured in DMEM / F12 medium containing the moxa smoke poison solution prepared by burning the moxa stick prepared in Example 1;

[0025] Figure 2 This is a morphological diagram of fibroblasts cultured in DMEM / F12 medium. DETAILED DESCRIPTION

[0026] The technical solution of the present invention will be further described below in conjunction with specific embodiments. However, it should be understood by those skilled in the art that the following examples are only intended to illustrate the present invention and should not be construed as limiting the present invention. Specific conditions not specified in the examples are to be followed according to conventional conditions or the conditions recommended by the manufacturer. All reagents or instruments used, unless otherwise specified, are conventional products obtained from commercial channels.

[0027] Example 1

[0028] A method for preparing a traditional Chinese medicine moxa stick for strengthening the kidney and nourishing the essence, specifically comprising the following steps:

[0029] (1) Disperse moxa in water (moxa to water mass ratio is 1:2), mix, treat with ultrasound (frequency 35 Hz, power 500 W, time 2 h), filter, dry, and set aside;

[0030] (2) Weighing the Chinese medicinal ingredients of Cistanche deserticola (10 parts), Epimedium (7 parts), Astragalus (7 parts), Chuanxiong (4 parts), and Angelica (3 parts) by weight, mixing them, crushing and sieving them to obtain Chinese medicinal powder;

[0031] (3) Take a certain amount of pullulan aqueous solution and mix it with 0.1 mol / L dilute sulfuric acid, adjust the pH to 4, add sodium periodate aqueous solution dropwise, the molar ratio of pullulan to sodium periodate is 1:1.5; stir the solution continuously for 5 hours under light-proof conditions, remove sodium periodate, and freeze-dry for 60 hours to obtain oxidized pullulan; dissolve the oxidized pullulan in anhydrous ethanol solvent, add theanine and mix, the molar ratio of oxidized pullulan to theanine is 1:1.3; add dilute hydrochloric acid dropwise, react at a temperature of 70°C for 3 hours, and remove the anhydrous ethanol by rotary evaporation to obtain modified pullulan. Mix the modified pullulan with paper mulberry bark powder in a mass ratio of 1:2 to obtain an adhesive;

[0032] (4) adding the adhesive obtained in step (3) to water (the mass ratio of the adhesive to deionized water is 1:2), and then adding the traditional Chinese medicine powder obtained in step (2) and mixing them uniformly to obtain a mixture;

[0033] (5) adding moxa to the mixture obtained in step (4), mixing thoroughly and evenly, pressing, and drying at normal pressure at a temperature of 70° C. to obtain moxa sticks.

[0034] The preparation method of paper mulberry bark powder mainly comprises the following steps: firstly, collecting healthy paper mulberry bark, cleaning it and cutting it into 1-5 mm segments, putting it into a 10% mass concentration NaOH solution, soaking it at 80°C for 40 minutes, removing the NaOH solution, washing it with water until it is neutral, and then crushing it after drying.

[0035] This embodiment also provides a traditional Chinese medicine moxa stick for strengthening the kidney and nourishing the essence obtained by the above preparation method.

[0036] Example 2

[0037] A method for preparing a traditional Chinese medicine moxa stick for strengthening the kidney and nourishing the essence, specifically comprising the following steps:

[0038] (1) Disperse moxa in water (moxa to water mass ratio is 1:2), mix, treat with ultrasound (frequency 20 Hz, power 400 W, time 1 h), filter, dry, and set aside;

[0039] (2) Weighing 8 parts of Cistanche deserticola, 5 parts of Epimedium, 5 parts of Astragalus, 3 parts of Chuanxiong, and 1 part of Angelica sinensis as the Chinese medicinal ingredients, mixing them, crushing and sieving them to obtain Chinese medicinal powder;

[0040] (3) Take a certain amount of pullulan aqueous solution and mix it with 0.1 mol / L dilute sulfuric acid, adjust the pH to 4, add sodium periodate aqueous solution dropwise, the molar ratio of pullulan to sodium periodate is 1:1; stir the solution continuously for 4 hours under light-proof conditions, remove sodium periodate, and freeze-dry for 48 hours to obtain oxidized pullulan; dissolve the oxidized pullulan in anhydrous ethanol solvent, add theanine and mix, the molar ratio of oxidized pullulan to theanine is 1:1.1; add dilute hydrochloric acid dropwise, react at a temperature of 60°C for 2 hours, and remove the anhydrous ethanol by rotary evaporation to obtain modified pullulan. Mix the modified pullulan with paper mulberry bark powder in a mass ratio of 1:1 to obtain an adhesive;

[0041] (4) adding the adhesive obtained in step (3) to water (the mass ratio of the adhesive to deionized water is 1:1), and then adding the traditional Chinese medicine powder obtained in step (2) and mixing them uniformly to obtain a mixture;

[0042] (5) adding moxa to the mixture obtained in step (4), mixing thoroughly and evenly, pressing, and drying at normal pressure at a temperature of 60° C. to obtain moxa sticks.

[0043] The preparation method of the paper mulberry bark powder is shown in Example 1.

[0044] This embodiment also provides a traditional Chinese medicine moxa stick for strengthening the kidney and nourishing the essence obtained by the above preparation method.

[0045] Example 3

[0046] A method for preparing a traditional Chinese medicine moxa stick for strengthening the kidney and nourishing the essence, specifically comprising the following steps:

[0047] (1) Disperse moxa in water (moxa to water mass ratio is 1:2), mix, treat with ultrasound (frequency 50 Hz, power 600 W, time 2 h), filter, dry, and set aside;

[0048] (2) Weigh the Chinese medicinal ingredients of Cistanche deserticola (12 parts), Epimedium (10 parts), Astragalus (10 parts), Chuanxiong (5 parts), and Angelica (4 parts) by weight, mix them, and grind and sieve them to obtain Chinese medicinal powder;

[0049] (3) Take a certain amount of pullulan aqueous solution and mix it with 0.1 mol / L dilute sulfuric acid, adjust the pH to 5, add sodium periodate aqueous solution dropwise, the molar ratio of pullulan to sodium periodate is 1:2; stir the solution continuously for 5 hours under light-proof conditions, remove sodium periodate, and freeze-dry for 72 hours to obtain oxidized pullulan; dissolve the oxidized pullulan in anhydrous ethanol solvent, add theanine and mix, the molar ratio of oxidized pullulan to theanine is 1:1.5; add dilute hydrochloric acid dropwise, react at a temperature of 80°C for 4 hours, and remove the anhydrous ethanol by rotary evaporation to obtain modified pullulan. Mix the modified pullulan with paper mulberry bark powder in a mass ratio of 1:2 to obtain an adhesive;

[0050] (4) adding the adhesive obtained in step (3) to water (the mass ratio of the adhesive to deionized water is 1:3), and then adding the traditional Chinese medicine powder obtained in step (2) and mixing them uniformly to obtain a mixture;

[0051] (5) adding moxa to the mixture obtained in step (4), mixing thoroughly and evenly, pressing, and drying at normal pressure at a temperature of 80° C. to obtain moxa sticks.

[0052] The preparation method of the paper mulberry bark powder is shown in Example 1.

[0053] This embodiment also provides a traditional Chinese medicine moxa stick for strengthening the kidney and nourishing the essence obtained by the above preparation method.

[0054] Comparative Example 1

[0055] The difference between Comparative Example 1 and Example 1 is that the modified pullulan in step (3) is replaced by pullulan.

[0056] Comparative Example 2

[0057] The difference between Comparative Example 2 and Example 1 is that the modified pullulan in step (3) is omitted.

[0058] Comparative Example 3

[0059] The difference between Comparative Example 3 and Example 1 is that the paper mulberry bark powder in step (3) is omitted.

[0060] Test Example 1

[0061] The moxa sticks prepared in Examples 1-3 and Comparative Examples 1-3 were each placed in a vibrating sieve machine equipped with a single-layer screen. The sieve size was set to 0.15 mm and the amplitude was 5 mm. After 5 minutes, the mass of the material under the sieve was weighed. The test was repeated three times, and the average value was taken to calculate the sieve drop rate. The sieve drop rate was calculated as follows: sieve drop rate = (mass of material under the sieve / mass of powder contained in the moxa stick) × 100%. The results are shown in Table 1.

[0062] Table 1 Screening rate

[0063]

[0064] As can be seen from Table 1, the sieve-dropping rate of the moxa sticks prepared in Examples 1-3 of the present invention is relatively low compared with Comparative Examples 1, 2, and 3. This indicates that the adhesive prepared by adding water to the modified pullulan and paper mulberry bark powder in Examples 1-3 plays a very important role in allowing the medicinal powder to adhere to the moxa, thereby solving the problem of the medicinal powder easily falling off during combustion, resulting in uneven release of the effective ingredients of traditional Chinese medicine.

[0065] Test Example 2

[0066] Moxa stick safety test:

[0067] (1) The moxa stick prepared in Example 1 was burned. The moxa smoke from the moxa stick burned in Example 1 was collected using a portable PM2.5 / PM10 sampling head after drying for 24 hours at room temperature. The smoke was then weighed after drying for 24 hours. The Cambridge filter was then dissolved in dimethyl sulfoxide to prepare a moxa smoke poison solution with a concentration of 15 mg / mL. The solution was filtered and sterilized, then aliquoted and stored in a refrigerator at -80°C for later use.

[0068] (2) The wormwood smoke poison solution prepared in step (1) was added to DMEM / F12 culture medium (containing 10% FBS, 100 U / mL penicillin, and 100 μg / mL streptomycin) and diluted 500 times to prepare DMEM / F12 culture medium containing the wormwood smoke poison solution.

[0069] (3) Mouse fibroblasts were collected and cultured in DMEM / F12 medium containing 10% FBS, 100 U / mL penicillin, and 100 μg / mL streptomycin in a 37°C, 5% CO2 cell culture incubator. When the mouse fibroblasts reached 70% confluency, they were digested with 0.25% trypsin, centrifuged at 2000 rpm for 10 min, resuspended, counted, and the cell density was adjusted to 5 × 104 cells / mL. 100 μL / well was seeded into 96-well plates.

[0070] (4) Remove the original culture medium from the 96-well plate and add 100 μL of DMEM / F12 culture medium prepared in step (2) to each well. For the control group, add DMEM / F12 culture medium containing 10% FBS, 100 U / mL penicillin, and 100 μg / mL streptomycin. Continue to culture for 24 hours and observe the cell morphology under a microscope. The results are as follows: Figure 1 、 Figure 2 shown.

[0071] The results are as follows Figure 1 、 Figure 2 The following are microscopic images of fibroblast morphology under different culture conditions. Figure 1 、 Figure 2It can be seen that the cell morphology of Example 1 is similar to that of the control group, showing normal mouse fibroblast morphology, and no damage is caused to the cell morphology, which indicates that the moxa stick prepared in Example 1 is safe.

[0072] In summary, the moxa stick prepared by the present invention can firmly adhere the medicinal powder to the moxa wool, solving the problems of easy detachment of the medicinal powder during combustion and uneven release of the effective ingredients of the traditional Chinese medicine, while also maintaining a stable columnar shape of the moxa stick. Furthermore, the process is simple, the conditions are mild, and the moxa stick is safe when fully burned.

[0073] Finally, it should be noted that the above embodiments are intended only to illustrate the technical solutions of the present invention and are not intended to limit the same. The basic principles and main features of the present invention have been described above using specific implementation schemes. Modifications or substitutions may be made based on the present invention, but such modifications or substitutions do not detract from the essence of the corresponding technical solutions from the scope of protection claimed by the present invention.

Claims

1. A method for preparing a traditional Chinese medicine moxa stick for strengthening the kidney and nourishing the essence, characterized in that: The following steps are involved: (1) Disperse moxa in water, treat with ultrasonic wave, filter, dry and set aside; (2) Mixing the Chinese medicinal ingredients of Cistanche deserticola, Epimedium, Astragalus, Chuanxiong, and Angelica sinensis, and crushing and sieving to obtain Chinese medicinal powder; (3) mixing the modified pullulan with paper mulberry bark powder to obtain an adhesive; (4) adding deionized water to the adhesive obtained in step (3), and then adding the Chinese medicinal powder obtained in step (2) and mixing them uniformly to obtain a mixture; (5) adding moxa to the mixture obtained in step (4), mixing thoroughly and evenly, and then pressing and drying to obtain moxa sticks.

2. The method for preparing the moxa stick for strengthening the kidney and nourishing essence according to claim 1, wherein: The preparation process of the modified pullulan is as follows: after mixing a pullulan aqueous solution with dilute sulfuric acid, adding a sodium periodate aqueous solution dropwise, stirring in the dark, removing the sodium periodate and freeze-drying to obtain oxidized pullulan; dissolving the oxidized pullulan in anhydrous ethanol, adding theanine, mixing and reacting to obtain the modified pullulan.

3. The method for preparing the moxa stick for strengthening the kidney and nourishing essence according to claim 2, wherein: The concentration of the dilute sulfuric acid is 0.08-0.12 mol / L; the molar ratio of the pullulan to sodium periodate is 1:(1-2); the light-proof stirring time is 4-6 hours; the freeze-drying time is 48-72 hours; the molar ratio of the oxidized pullulan to theanine is 1:(1.1-1.5); after the addition of theanine and mixing, the reaction temperature is 60-80°C, and the reaction time is 2-4 hours.

4. The method for preparing the moxa stick for strengthening the kidney and nourishing essence according to claim 1, wherein: The frequency of the ultrasonic treatment in step (1) is 20-50 KHZ, the power is 400-600 W, and the time is 1-2 hours.

5. The method for preparing the moxa stick for strengthening the kidney and nourishing essence according to claim 1, wherein: The weight proportions of the Chinese medicinal ingredients in step (2) are: 8-12 parts of Cistanche deserticola, 5-10 parts of Epimedium, 5-10 parts of Astragalus, 3-5 parts of Chuanxiong, and 1-4 parts of Angelica sinensis.

6. The method for preparing the traditional Chinese medicine moxa stick for strengthening the kidney and nourishing essence according to claim 1, wherein: In the step (3), the mass ratio of the modified pullulan to the paper mulberry bark powder is 1:(1-3).

7. The method for preparing the moxa stick for strengthening the kidney and nourishing the essence according to claim 1, wherein: The mass ratio of the adhesive to the deionized water in the step (4) is 1:(1-3).

8. The method for preparing the traditional Chinese medicine moxa stick for strengthening the kidney and nourishing essence according to claim 1, wherein: The drying in step (5) is performed under normal pressure at a temperature of 60-80°C.

9. A traditional Chinese medicine moxa stick for strengthening the kidney and nourishing the essence, characterized in that: Prepared according to the preparation method according to any one of claims 1 to 8.

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