Micro-fluidic chip clamping kettle capable of being combined with Raman spectrum

Abstract

The invention discloses a micro-fluidic chip clamping kettle capable of being combined with a Raman spectrum, belongs to the technical field of micro-fluidic chip detection auxiliary equipment, and aims to solve the problem that an existing micro-fluidic chip clamping kettle is difficult to meet the detection requirement of the Raman spectrum. The micro-fluidic chip clamping kettle capable of being combined with the Raman spectrum comprises an upper cover (1), a kettle body (2) and a lower cover (3) which are sequentially arranged from top to bottom, and the upper cover (1), the kettle body (2) and the lower cover (3) are of annular structures. The micro-fluidic chip clamping kettle capable of being combined with the Raman spectrum can be combined with the Raman spectrum for detection, so that the application range of the micro-fluidic chip is expanded, and the practicability and the detection means of the micro-fluidic chip are improved.

Description

Technical Field

[0001] The invention relates to the technical field of microfluidic chip detection auxiliary equipment, in particular to a microfluidic chip clamping kettle capable of being used in conjunction with Raman spectroscopy. Background Art

[0002] Microfluidics refers to the precise control and processing of fluids using microchannels. As an emerging technology, it has rapidly developed in recent years in research fields such as biology and chemical engineering. Microfluidic chips, as core components of microfluidics, can perform various biochemical reactions, separations, and detection processes on-chip. Compared to traditional amplification reaction methods, microfluidic chips offer advantages such as high integration, compact size, high experimental efficiency, and low reagent consumption. Therefore, microfluidics represents a new direction in chemical laboratory technology and testing and inspection techniques.

[0003] In the prior art, microfluidic chips are usually used in conjunction with corresponding clamping kettles during detection. The clamping kettle is used to fix the corresponding chip, and at the same time, the auxiliary microfluidic chip is connected to the microfluidic pump to supply liquid and pressurize the microfluidic chip. At present, the observation method of microfluidic technology is mainly combined with microscopic imaging technology for observation, which cannot realize the analysis and detection of the material composition in the flow channel of the microfluidic chip, which greatly limits the scope of application of microfluidic technology. As a microscopic detection technology that does not damage the sample, Raman spectrometer can realize in-situ real-time monitoring of material composition and has good adaptability to microfluidic chips. However, the microfluidic chip clamping kettles currently on the market are difficult to meet the detection requirements of Raman spectroscopy. Summary of the Invention

[0004] In order to solve the problem that the existing microfluidic chip clamping kettle is difficult to meet the detection requirements of Raman spectroscopy, the present invention provides a microfluidic chip clamping kettle that can be used in conjunction with Raman spectroscopy. The upper cover, kettle body and lower cover of the microfluidic chip clamping kettle that can be used in conjunction with Raman spectroscopy are all annular structures, which can be used in conjunction with Raman spectroscopy for detection, thereby expanding the scope of application of microfluidic chips and increasing their practicality and detection methods.

[0005] The technical solution adopted by the present invention to solve its technical problem is:

[0006] A microfluidic chip clamping kettle capable of being used in conjunction with Raman spectroscopy comprises an upper cover, a kettle body and a lower cover which are arranged in sequence from top to bottom, the upper cover, the kettle body and the lower cover are all annular structures, the upper cover comprises an upper cover wall and an upper cover inner cavity, the kettle body comprises a kettle body wall and an inner kettle cavity, the lower cover comprises a lower cover wall and a lower cover inner cavity, the upper cover inner cavity, the kettle inner cavity and the lower cover inner cavity are sequentially connected from top to bottom, an upper transparent cover plate is arranged in the upper cover inner cavity, a guard plate is arranged in the kettle inner cavity, the guard plate is annular in structure, comprises a guard plate wall and a chip mounting cavity, the microfluidic chip can be matched and arranged in the chip mounting cavity, a sample liquid inlet flow channel and a sample liquid outlet flow channel are arranged in the kettle body wall, the inlet of the microfluidic chip can be communicated with the sample liquid inlet flow channel, the outlet of the microfluidic chip can be communicated with the sample liquid outlet flow channel, and a lower transparent cover plate is arranged in the lower cover inner cavity.

[0007] An inner annular protrusion is provided on the inner circumference surface of the kettle body wall, and the cavity inside the kettle is divided into an upper cavity inside the kettle and a lower cavity inside the kettle by the inner annular protrusion. The guard plate and the kettle body have a transition fit or a clearance fit, and the guard plate and the inner annular protrusion are connected in a stacked manner up and down. The microfluidic chip can be connected in a stacked manner up and down with the inner annular protrusion.

[0008] A first through hole is provided in the guard plate wall of the guard plate, and a second through hole is provided in the inner annular protrusion. The upper cavity in the kettle is connected with the lower cavity in the kettle through the first through hole and the second through hole in sequence. The outlet end of the sample liquid inlet flow channel and the inlet end of the sample liquid outlet flow channel are both located on the upper surface of the inner annular protrusion.

[0009] The lower end of the upper cover wall is inserted into the upper cavity in the kettle, the upper cover and the kettle body are sealed, there is a distance between the lower end of the upper cover wall and the guard plate, and the upper cavity liquid inlet and the upper cavity purge port are provided on the upper cover wall, and the upper cavity liquid inlet and the upper cavity purge port are both connected to the upper cavity in the kettle.

[0010] The upper transparent cover is inserted into the lower end of the cavity in the upper cover. The upper transparent cover is sealed to the upper cover. The upper transparent cover is made of sapphire glass. The upper transparent cover is connected to the upper cover through an upper gasket. A fixing component is provided in the cavity in the kettle. The fixing component can fix the microfluidic chip in the chip mounting cavity.

[0011] The upper end of the lower cover wall is inserted into the lower cavity in the kettle, the lower cover and the kettle body are sealed, there is a distance between the upper end of the lower cover wall and the inner annular protrusion, and a lower cavity outlet is provided on the lower cover wall, and the lower cavity outlet is connected to the lower cavity in the kettle.

[0012] The lower transparent cover is inserted into the upper end of the cavity in the lower cover. The lower transparent cover is sealed to the lower cover. The lower transparent cover is made of sapphire glass and is connected to the lower cover through a lower gasket.

[0013] The axis of the upper cover, the axis of the kettle body and the axis of the lower cover coincide with each other. The upper cover and the lower cover are connected to the kettle body by bolts. A water bath inlet and a water bath outlet are provided on the kettle body wall, and a water bath flow channel is provided inside the kettle body wall.

[0014] The microfluidic chip clamping kettle capable of being used in conjunction with Raman spectroscopy also includes a position adjustment mechanism. In a spatial rectangular coordinate system with X-axis, Y-axis and Z-axis as coordinate axes, the position adjustment mechanism can move the upper cover, the kettle body and the lower cover along the X-axis direction, the Y-axis direction and the Z-axis direction.

[0015] The position adjustment mechanism includes a Z-axis moving component, an X-axis moving component and a Y-axis moving component. The Z-axis moving component includes a storage platform, a shell and a Z-axis direction moving knob. Rotating the Z-axis direction moving knob can move the storage platform up and down. A scale is provided on the shell. The lower cover, Y-axis moving component and X-axis moving component are connected in sequence from top to bottom.

[0016] The beneficial effects of the present invention are:

[0017] 1. The upper cover, the body and the lower cover of the microfluidic chip clamping kettle that can be used in conjunction with Raman spectroscopy are all annular structures, ensuring that the vertical distance from the upper cover to the microfluidic chip meets the effective working focal length of the Raman spectrometer. It can be used in conjunction with Raman spectroscopy for detection, thereby expanding the scope of application of the microfluidic chip and increasing its practicality and detection methods.

[0018] 2. Add an adjustable bracket to facilitate the Raman spectrometer and microscope to focus on different positions of the chip, increasing the operability of the experiment.

[0019] 3. While ensuring the safety pressure of the experiment, the size of the sapphire window on the upper cover is expanded to facilitate the adjustment of the Raman lens and microscope lens. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The drawings in the specification, which constitute a part of this application, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0021] Figure 1 This is a schematic diagram of the main view of the microfluidic chip clamping kettle that can be used in conjunction with Raman spectroscopy according to the present invention.

[0022] Figure 2 This is a schematic diagram of the upper cover.

[0023] Figure 3 It is a schematic diagram of the kettle body.

[0024] Figure 4 This is a schematic diagram of the lower cover.

[0025] Figure 5 Schematic diagram of the microfluidic chip.

[0026] Figure 6 It is a top view schematic diagram of the guard plate.

[0027] Figure 7 1 is a top view schematic diagram of the microfluidic chip clamping kettle capable of being combined with Raman spectroscopy after the upper cover is removed.

[0028] Figure 8 It is a top view schematic diagram of the X-axis moving component and the Y-axis moving component.

[0029] Figure 9 This is a schematic diagram of the Z-axis movement component.

[0030] Description of reference numerals:

[0031] 1. Upper cover; 2. Kettle body; 3. Lower cover; 4. Upper transparent cover; 5. Guard plate; 6. Lower transparent cover; 7. Fixing components; 8. Position adjustment mechanism; 9. Microfluidic chip;

[0032] 101. Upper cover wall; 102. Upper cover inner cavity; 103. Upper cavity liquid inlet; 104. Upper cavity purge port;

[0033] 201, kettle body wall; 202, kettle cavity; 203, sample liquid inlet flow channel; 204, sample liquid outlet flow channel; 205, inner annular protrusion; 206, kettle upper cavity; 207, kettle lower cavity; 208, second through hole; 208, second through hole; 209, water bath outlet; 2010, water bath inlet;

[0034] 301, lower cover wall; 302, lower cover inner cavity; 303, lower cavity outlet;

[0035] 401, upper gasket;

[0036] 501, guard plate wall; 502, chip mounting cavity; 503, first through hole; 504, third through hole; 505, fourth through hole;

[0037] 601, lower gasket;

[0038] 801, Z-axis moving assembly; 802, X-axis moving assembly; 803, Y-axis moving assembly;

[0039] 901, inlet; 902, outlet; 903, sample pool;

[0040] 8011, storage platform; 8012, housing; 8013, Z-axis movement knob; 8014, scale;

[0041] 8021, X-axis direction movement knob; 8022, X-axis screw; 8023, X-axis base;

[0042] 8031, Y-axis movement knob; 8032, Y-axis lead screw; 8033, Y-axis base. Detailed implementation mode

[0043] It should be noted that, without conflict, the embodiments in this application and the features in the embodiments can be combined with each other. The following will describe the present invention in detail with reference to the drawings and in combination with the embodiments.

[0044] For the convenience of understanding and description, the following description of the present invention adopts an absolute position relationship. Without special explanation, the orientation word "up" represents Figure 1 the upper side direction in Figure 1 the orientation word "down" represents Figure 1 the lower side direction in Figure 1 the orientation word "left" represents Figure 1 the left side direction in Figure 1 the orientation word "right" represents

[0045] such as Figures 1 to 5 shown, a microfluidic chip clamping kettle capable of being used in combination with Raman spectroscopy described in the embodiment of the present invention includes an upper cover 1, a kettle body 2 and a lower cover 3 connected in sequence from top to bottom. The upper cover 1, the kettle body 2 and the lower cover 3 are all annular structures. The upper cover 1 includes an upper cover wall 101 and an upper cover inner cavity ......

[0046] such as Figure 3As shown, the inner circumferential surface of the kettle body wall 201 is provided with an inner annular protrusion 205, and the inner cavity 202 of the kettle is divided into an upper cavity 206 and a lower cavity 207 in the kettle by the inner annular protrusion 205. The upper cavity 206 and the lower cavity 207 in the kettle are arranged at intervals in the upper and lower directions. The guard plate 5 is located in the upper cavity 206 in the kettle, and the guard plate 5 and the kettle body 2 have a transition fit or a clearance fit. The guard plate 5 and the inner annular protrusion 205 are connected in a stacked manner. The microfluidic chip 9 can be installed in the chip mounting cavity 502 in a matching manner, and the microfluidic chip 9 can be connected in a stacked manner in the inner annular protrusion 205.

[0047] like Figure 1 and Figure 6 As shown, a plurality of first through holes 503 are provided in the guard plate wall 501 of the guard plate 5, and a plurality of second through holes 208 are provided in the inner annular protrusion 205. The first through holes 503 and the second through holes 208 correspond to each other one by one. The upper cavity 206 in the kettle is connected to the lower cavity 207 in the kettle through the first through holes 503 and the second through holes 208 in sequence. The outlet end of the sample liquid inlet flow channel 203 and the inlet end of the sample liquid outlet flow channel 204 are both located on the upper surface of the inner annular protrusion 205. The inlet 901 of the microfluidic chip 9 can be connected to the outlet end of the sample liquid inlet flow channel 203, and the outlet 902 of the microfluidic chip 9 can be connected to the inlet end of the sample liquid outlet flow channel 204.

[0048] like Figure 1 As shown, the lower end of the upper cover wall 101 is matingly inserted into the upper cavity 206 within the kettle. The upper cover 1 and the kettle body 2 are sealed together via a sealing ring. A gap exists between the lower end of the upper cover wall 101 and the guard plate 5. The upper cover wall 101 is provided with an upper cavity liquid inlet 103 and an upper cavity purge port 104, both of which are connected to the upper cavity 206 within the kettle. The simple structure of the input and purge system helps to improve the safety of the microfluidic chip and the safety of experimental operations.

[0049] The upper transparent cover plate 4 is matingly inserted into the lower end of the upper cover cavity 102. The upper transparent cover plate 4 and the upper cover 1 are sealed together via a sealing ring. The upper transparent cover plate 4 can be made of sapphire glass and is connected to the upper cover 1 via an upper gasket 401. A fixing component 7 is disposed within the inner cavity 202 of the kettle. The fixing component 7 can secure the microfluidic chip 9 within the chip mounting cavity 502. The upper gasket 401 is a carbon dioxide corrosion-resistant gasket that can be used to seal the carbon dioxide intake channel, contributing to long-term sealing during the carbon dioxide intake process.

[0050] For example, the combined thickness of the upper transparent cover plate 4 and upper gasket 401 can be 12.5 mm, enabling microscopic or Raman spectroscopy detection of samples in the sample reservoir 903 of the microfluidic chip 9 clamped in the kettle. Furthermore, the pressure input system can apply precise pressures within the range of 1 MPa to 15 MPa to the upper cavity 206 and lower cavity 207 within the kettle body to control the flow of liquid or gas within the microfluidic chip and protect the microfluidic chip 9. Furthermore, the height of the upper cavity 206 within the kettle is set to 3 mm, and the height of the microfluidic chip 9 is set to 4 mm, resulting in a distance of 19.5 mm from the Raman spectroscopy lens to the bottom of the sample.

[0051] like Figure 1 、 Figures 5 to 7 As shown, a third through hole 504 and a fourth through hole 505 are provided in the guard plate wall 501 of the guard plate 5. The fixing component 7 is an elongated structure. Multiple fixing components 7 are evenly spaced along the circumference of the guard plate 5. The fixing component 7 is connected and fixed to the guard plate 5 by a first screw. The fixing component 7 and the guard plate 5 are stacked and connected vertically. The fixing component 7 can be stacked and connected vertically with the microfluidic chip 9. There is a distance between the fixing component 7 and the upper transparent cover plate 4. The tail end of the first screw is located in the third through hole 504. The guard plate 5 can be connected and fixed to the inner annular protrusion 205 by a second screw. The tail end of the second screw is located in the fourth through hole 505.

[0052] The upper end of the lower cover wall 301 is matched and inserted into the lower cavity 207 in the kettle. The lower cover 3 and the kettle body 2 are sealed by a sealing ring. There is a distance between the upper end of the lower cover wall 301 and the inner annular protrusion 205. A lower cavity outlet 303 is provided on the lower cover wall 301, and the lower cavity outlet 303 is connected to the lower cavity 207 in the kettle.

[0053] like Figure 1 As shown, the lower transparent cover plate 6 is matingly inserted into the upper end of the cavity 302 in the lower cover. The lower transparent cover plate 6 and the lower cover 3 are sealed together by a sealing ring. The material of the lower transparent cover plate 6 can be sapphire glass. The lower transparent cover plate 6 is connected to the lower cover 3 via a lower gasket 601. The lower transparent cover plate 6 and the cavity 302 in the lower cover are provided for supplementary light during microscopic observation.

[0054] The axis of the upper cover 1, the axis of the kettle body 2, the axis of the lower cover 3, the axis of the upper transparent cover plate 4, the axis of the guard plate 5, the axis of the lower transparent cover plate 6, and the axis of the pressure ring 702 coincide. The upper cover 1 and the lower cover 3 are both connected to the kettle body 2 by bolts. A water bath inlet 2010 and a water bath outlet 209 are provided on the kettle body wall 201. A water bath flow channel is provided within the kettle body wall 201. The water bath inlet 2010 and the water bath outlet 209 are both connected to the water bath flow channel. A water bath device can be connected to the water bath inlet 2010 and the water bath outlet 209 to control the temperature of the microfluidic chip or a local area.

[0055] like Figures 8 and 9 As shown, the microfluidic chip clamping kettle that can be used in conjunction with Raman spectroscopy also includes a position adjustment mechanism 8. In a spatial rectangular coordinate system with X-axis, Y-axis and Z-axis as coordinate axes, the position adjustment mechanism 8 can move the upper cover 1, the kettle body 2 and the lower cover 3 along the X-axis direction, the Y-axis direction and the Z-axis direction.

[0056] The position adjustment mechanism 8 includes a Z-axis moving component 801, an X-axis moving component 802 and a Y-axis moving component 803. The Z-axis moving component 801 can move the upper cover 1, the kettle body 2 and the lower cover 3 along the Z-axis direction (up and down direction), the X-axis moving component 802 can move the upper cover 1, the kettle body 2 and the lower cover 3 along the X-axis direction (left and right direction), and the Y-axis moving component 803 can move the upper cover 1, the kettle body 2 and the lower cover 3 along the Y-axis direction (front and back direction).

[0057] like Figure 9 As shown, the Z-axis moving assembly 801 includes a storage platform 8011, a shell 8012 and a Z-axis moving knob 8013. The Z-axis moving knob 8013 is connected to the storage platform 8011 through a screw-nut transmission mechanism. Rotating the Z-axis moving knob 8013 can move the storage platform 8011 up and down. A scale 8014 is provided on the shell 8012, and the scale 8014 can display the moving distance of the storage platform 8011. The upper cover 1, the kettle body 2, the lower cover 3, the Y-axis moving assembly 803, the X-axis moving assembly 802 and the storage platform 8011 are connected in sequence from top to bottom.

[0058] like Figure 8 As shown, the Y-axis movement assembly 803 includes a Y-axis base 8033, a Y-axis direction movement knob 8031, a Y-axis screw 8032, a Y-axis nut, and a Y-axis slide, which are connected in sequence. The X-axis movement assembly 802 includes an X-axis base 8023, an X-axis direction movement knob 8021, an X-axis screw 8022, an X-axis nut, and an X-axis slide, which are connected in sequence. Rotating the Y-axis direction movement knob 8031 can move the Y-axis nut and the Y-axis slide forward and backward, while rotating the X-axis direction movement knob 8021 can move the X-axis nut and the X-axis slide left and right. The lower cover 3 is connected to the Y-axis slide up and down by bolts, the Y-axis base 8033 is connected to the X-axis slide up and down by bolts, and the X-axis base 8023 is connected to the storage platform 8011 up and down by bolts.

[0059] The microfluidic chip clamping kettle that can be used in conjunction with Raman spectroscopy contains a temperature control system. In actual applications, the temperature control system provides specific temperature conditions for the biochemical reactions of the microfluidic chip. At the same time, because the fluid movement in the microfluidic chip follows the laws of microscopic fluid mechanics and the flow rate is easily affected by external forces, a microfluidic pump system is integrated into the clamping kettle body. The microfluidic pump system is used to accurately apply pressure to the fluid in the microfluidic control and control the injection speed, thereby increasing the test speed, improving the test accuracy, and improving the precise control of the test process on the microfluidic chip. In addition, it also enables the microfluidic chip to meet the work requirements of more biochemical reactions, separations, detection, and other operations, thereby effectively broadening the scope of application of the microfluidic chip and enhancing its practicality.

[0060] The following describes the working process of the microfluidic chip clamping kettle that can be used in conjunction with Raman spectroscopy.

[0061] The microfluidic chip 9 to be tested is installed in the chip mounting cavity 502 of the protective plate 5. The microfluidic chip 9 includes an inlet 901, an outlet 902, and a sample reservoir 903 (i.e., a microfluidic channel). The upper cover 1, the kettle body 2, and the lower cover 3 are sequentially connected and secured to the Y-axis slide of the Y-axis moving assembly 803 of the position adjustment mechanism 8 (the lower cover 3 is bolted to the Y-axis slide). The X-axis base 8023 is bolted to the storage platform 8011. The position adjustment mechanism 8 is then secured to the optical platform of the Raman spectroscopy apparatus. The Raman spectroscopy receiving device is mounted between the lower cover 3 and the Y-axis slide, with the cavity 302 in the lower cover aligning with the Raman spectroscopy receiving device.

[0062] The water bath inlet 2010 and outlet 209 are connected to a water bath, which is used to adjust the clamped kettle to the appropriate experimental temperature. The upper cavity liquid inlet 103 and lower cavity outlet 303 are connected to a first pressure input system, which can inject pressurized liquid into the upper cavity 206 and lower cavity 207 within the kettle. This first pressure input system is used to apply pressure to the outside of the microfluidic chip 9. The sample liquid inlet flow channel 203 and sample liquid outlet flow channel 204 are connected to a second pressure input system, which is used to apply pressure and inject sample liquid into the microfluidic chip 9 during the experiment.

[0063] Afterwards, the position adjustment mechanism 8 is used to focus the sample pool 903 under the Raman spectroscopic lens to locate the desired detection area. The Raman spectroscopic laser then scans and detects the liquid sample in the sample pool 903 through the upper transparent cover plate 4 and the upper cavity 206 within the kettle. After the experiment is complete, the sample liquid outlet channel 204 is opened to reduce the pressure within the microfluidic chip, or the sample liquid inlet channel 203 is connected to a pressure input system to purge the sample from the sample pool 903. Finally, the upper cavity purge port 104 is connected to a third pressure input system, allowing the water in the upper cavity 206 and lower cavity 207 within the kettle to be discharged through the lower cavity outlet 303. After the experiment is complete, the microfluidic chip 9 can be removed.

[0064] This approach improves the integration of the microfluidic clamping vessel, enhances the controllability and monitoring of the microfluidic chip 9 during experiments, and integrates multiple manufacturing and monitoring processes into a single location, effectively increasing the efficiency of testing the microfluidic chip 9. Furthermore, combining the clamping vessel with Raman spectroscopy equipment significantly enhances the accuracy of sample detection in the sample pool 903 within the microfluidic clamping vessel, broadening the application range of the microfluidic chip 9.

[0065] The above description is merely a specific embodiment of the present invention and is not intended to limit the scope of the invention. Therefore, substitutions of equivalent components, or equivalent changes and modifications made within the scope of protection of the present invention, should still fall within the scope of the present invention. Furthermore, the technical features of the present invention may be freely combined with each other, with each other's technical solutions, and with each other's technical solutions.

Claims

1. A microfluidic chip clamping kettle capable of being used in conjunction with Raman spectroscopy, characterized in that: The microfluidic chip clamping kettle capable of being used in conjunction with Raman spectroscopy comprises an upper cover (1), a kettle body (2) and a lower cover (3) which are sequentially arranged from top to bottom. The upper cover (1), the kettle body (2) and the lower cover (3) are all annular structures. The upper cover (1) comprises an upper cover wall (101) and an upper cover inner cavity (102). The kettle body (2) comprises a kettle body wall (201) and an inner cavity (202). The lower cover (3) comprises a lower cover wall (301) and an inner cavity (302). The inner cavity (102) of the upper cover, the inner cavity (202) of the kettle and the inner cavity (302) of the lower cover are sequentially connected from top to bottom. An upper transparent cover plate (4) is arranged in the inner cavity (102) of the upper cover. A guard plate (5) is provided in the cavity (202) in the kettle. The guard plate (5) is an annular structure. The guard plate (5) includes a guard plate wall (501) and a chip mounting cavity (502). The microfluidic chip (9) can be matched and arranged in the chip mounting cavity (502). A sample liquid inlet flow channel (203) and a sample liquid outlet flow channel (204) are provided in the kettle body wall (201). The inlet (901) of the microfluidic chip (9) can be communicated with the sample liquid inlet flow channel (203), and the outlet (902) of the microfluidic chip (9) can be communicated with the sample liquid outlet flow channel (204). A lower transparent cover plate (6) is provided in the cavity (302) in the lower cover.

2. The microfluidic chip clamping kettle capable of being used in conjunction with Raman spectroscopy according to claim 1, characterized in that: An inner annular protrusion (205) is provided on the inner peripheral surface of the kettle body wall (201); the inner cavity (202) of the kettle is divided into an upper cavity (206) and a lower cavity (207) of the kettle by the inner annular protrusion (205); the guard plate (5) and the kettle body (2) are in transitional fit or clearance fit; the guard plate (5) and the inner annular protrusion (205) are connected in a stacked manner; and the microfluidic chip (9) can be connected in a stacked manner to the inner annular protrusion (205).

3. The microfluidic chip clamping kettle capable of being used in conjunction with Raman spectroscopy according to claim 2, characterized in that: A first through hole (503) is provided in the guard plate wall (501) of the guard plate (5), and a second through hole (208) is provided in the inner annular protrusion (205). The upper cavity (206) in the kettle is connected to the lower cavity (207) in the kettle through the first through hole (503) and the second through hole (208) in sequence. The outlet end of the sample liquid inlet flow channel (203) and the inlet end of the sample liquid outlet flow channel (204) are both located on the upper surface of the inner annular protrusion (205).

4. The microfluidic chip clamping kettle capable of being used in conjunction with Raman spectroscopy according to claim 2, characterized in that: The lower end of the upper cover wall (101) is inserted into the upper cavity (206) in the kettle, the upper cover (1) and the kettle body (2) are sealed, and there is a distance between the lower end of the upper cover wall (101) and the guard plate (5). The upper cavity liquid inlet (103) and the upper cavity purge port (104) are provided on the upper cover wall (101), and the upper cavity liquid inlet (103) and the upper cavity purge port (104) are both connected to the upper cavity (206) in the kettle.

5. The microfluidic chip clamping kettle capable of being used in conjunction with Raman spectroscopy according to claim 1, characterized in that: The upper transparent cover plate (4) is inserted into the lower end of the cavity (102) in the upper cover, and the upper transparent cover plate (4) is sealed and connected to the upper cover (1). The upper transparent cover plate (4) is made of sapphire glass and is connected to the upper cover (1) via an upper gasket (401). A fixing component (7) is provided in the cavity (202) in the kettle, and the fixing component (7) can fix the microfluidic chip (9) in the chip mounting cavity (502).

6. The microfluidic chip clamping kettle capable of being used in conjunction with Raman spectroscopy according to claim 2, characterized in that: The upper end of the lower cover wall (301) is inserted into the lower cavity (207) in the kettle, the lower cover (3) and the kettle body (2) are sealed, there is a distance between the upper end of the lower cover wall (301) and the inner annular protrusion (205), and a lower cavity outlet (303) is provided on the lower cover wall (301), and the lower cavity outlet (303) is connected to the lower cavity (207) in the kettle.

7. The microfluidic chip clamping kettle capable of being used in conjunction with Raman spectroscopy according to claim 1, characterized in that: The lower transparent cover plate (6) is inserted into the upper end of the cavity (302) in the lower cover. The lower transparent cover plate (6) and the lower cover (3) are sealed and connected. The material of the lower transparent cover plate (6) is sapphire glass. The lower transparent cover plate (6) is connected to the lower cover (3) via a lower gasket (601).

8. The microfluidic chip clamping kettle capable of being used in conjunction with Raman spectroscopy according to claim 1, characterized in that: The axis of the upper cover (1), the axis of the kettle body (2) and the axis of the lower cover (3) coincide with each other; the upper cover (1) and the lower cover (3) are connected to the kettle body (2) by bolts; a water bath outlet (209) and a water bath inlet (2010) are provided on the kettle body wall (201); and a water bath flow channel is provided in the kettle body wall (201).

9. The microfluidic chip clamping reactor capable of being used in conjunction with Raman spectroscopy according to claim 1, characterized in that: The microfluidic chip clamping kettle capable of being used in conjunction with Raman spectroscopy further comprises a position adjustment mechanism (8). In a spatial rectangular coordinate system having an X-axis, a Y-axis and a Z-axis as coordinate axes, the position adjustment mechanism (8) is capable of moving the upper cover (1), the kettle body (2) and the lower cover (3) along the X-axis direction, the Y-axis direction and the Z-axis direction.

10. The microfluidic chip clamping kettle capable of being used in conjunction with Raman spectroscopy according to claim 9, characterized in that: The position adjustment mechanism (8) comprises a Z-axis moving assembly (801), an X-axis moving assembly (802) and a Y-axis moving assembly (803); the Z-axis moving assembly (801) comprises a storage platform (8011), a housing (8012) and a Z-axis direction moving knob (8013); rotating the Z-axis direction moving knob (8013) enables the storage platform (8011) to move up and down; a scale (8014) is provided on the housing (8012); and the lower cover (3), the Y-axis moving assembly (803) and the X-axis moving assembly (802) are connected in sequence from top to bottom.

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