Histamine removal and extraction equipment and method for apramycin sulfate

Abstract

The invention relates to the technical field of histamine extraction, in particular to histamine removal and extraction equipment and method for apramycin sulfate, the histamine removal and extraction equipment comprises a drying box with a feeding port and a discharging port, an opening and closing unit used for controlling opening and closing of the discharging port is arranged at the discharging port, and a plurality of heating plates sequentially arranged in the horizontal direction are arranged in the drying box; a heating cavity is defined by every two adjacent heating plates; a sliding plate and a driving unit used for driving the sliding plate to move in the length direction of the heating cavity are arranged in the drying box, a plurality of air injection units and a plurality of vertically-arranged spiral conveying units are arranged on the sliding plate, and every two sets of air injection units correspond to one heating cavity and vertically blow air to the cavity walls of the left side and the right side of the heating cavity correspondingly. And the spiral conveying units are in one-to-one correspondence with the heating cavities and extend into the corresponding heating cavities. The drying efficiency of the crystals can be improved.

Description

Technical Field

[0001] The present application relates to the technical field of histamine extraction, and in particular to a histamine removal and extraction device and method for apramycin sulfate. Background Art

[0002] Apramycin sulfate is an aminoglycoside antibiotic primarily used to prevent and treat bacterial infections in livestock and poultry, such as digestive or respiratory diseases caused by Escherichia coli and Salmonella. Apramycin sulfate is produced through microbial fermentation, possibly using aminoglycoside-producing actinomycetes. Post-fermentation extraction and purification processes may produce byproducts or impurities, such as histamine. The presence of histamine can affect the purity of apramycin sulfate, necessitating the removal of histamine through extraction.

[0003] During the histamine extraction process, the apramycin sulfate fermentation broth needs to be centrifuged to remove bacterial residues and insoluble impurities, then the histamine is eluted using an ion exchange resin method, and then the crude histamine is obtained by solvent extraction. Finally, the crude histamine is crystallized and dried to obtain the finished histamine product.

[0004] Through searching, Chinese patent announcement number CN220931578U discloses a vacuum drying device for preparing acid salt, comprising a drying tower body, wherein the inner walls around the drying tower body are fixed with a placement plate by bolts, the upper surface of the placement plate is rotatably connected to a connecting shaft, and a plurality of stirring plates are welded to the circumferential outer wall of the connecting shaft, and a plurality of heating wires are provided between adjacent stirring plates, and one side of the plurality of stirring plates is rotatably connected to two spiral racks, and the thread directions of the two spiral racks are opposite. The utility model can not only change the fixed state of the crystals during drying by setting the stirring plates, so that the crystals are heated evenly, but also circulate the crystals by setting the stirring racks, thereby improving the fluidity of the crystals and making the crystals heated more evenly, and can also discharge the crystals from the drying chamber by setting the discharging mechanism, making the discharging more convenient and improving the convenience of the device.

[0005] The above-mentioned related technologies have the following defects: although the spiral rack can transport and stir the crystals, since the heating wire is arranged at the stirring plate, the crystals located between the upper and lower spiral racks and the crystals located on the inner wall of the drying tower body are not easily transported to the heating wire for drying, resulting in low drying efficiency of the crystals; in addition, during the unloading process of the crystals, the spiral rack cannot clean the crystals located between the two spiral racks and attached to the inner wall of the drying tower body, resulting in incomplete discharge of the crystals. At this time, workers need to clean them manually, which further affects the drying efficiency of the crystals, and therefore needs to be improved. Summary of the Invention

[0006] In order to improve the drying efficiency of crystals, the present application provides a histamine removal and extraction device and method for apramycin sulfate.

[0007] In a first aspect, the present application provides an apparatus for removing histamine from apramycin sulfate, which employs the following technical solution: The apparatus comprises a drying oven having a feed inlet and a discharge outlet, wherein an opening and closing unit for controlling the opening and closing of the discharge outlet is provided at the discharge outlet, and wherein a plurality of heating plates arranged in sequence in a horizontal direction are provided within the drying oven, wherein every two adjacent heating plates together form a heating chamber;

[0008] The drying box is equipped with a slide and a driving unit for driving the slide to move along the length direction of the heating chamber. The slide is equipped with several jet units and several vertically arranged spiral conveying units. Every two groups of jet units correspond to one heating chamber and blow vertically toward the left and right side walls of the heating chamber respectively. The spiral conveying units correspond to the heating chambers one by one and extend into the corresponding heating chambers.

[0009] Optionally, the spiral conveying unit includes a vertically arranged spiral blade, which rotates around its own axis and is connected to the slide, and every two adjacent spiral blades are connected by a belt drive.

[0010] Optionally, the side of the drying box is connected to a connection box, and a fixed rack is provided in the connection box, and the length direction of the fixed rack is the same as the length direction of the heating chamber;

[0011] A transmission gear set is provided on the skateboard, the transmission gear set is connected to one of the spiral leaves located on the edge, and the transmission gear set is engaged with the fixed rack.

[0012] Optionally, a turntable is provided on the spiral blade, the side walls of the turntable are in contact with the left and right walls of the heating chamber, and the lower surface of the turntable is in contact with the opening and closing unit that closes the discharge port.

[0013] Optionally, both the front and rear ends of the heating chamber are provided with concave cavities, the cross section of the concave cavities is semicircular and is for the spiral blades to be rotatably embedded.

[0014] Optionally, a plurality of downwardly inclined air blowing ports are provided on the cavity wall of one of the concave cavities, and an air blowing unit connected to all the air blowing ports is provided on the drying box.

[0015] Optionally, the driving unit includes a driving motor provided on the drying box, and a screw rod is coaxially connected to the output shaft of the driving motor. The screw rod rotates around its own axis and is connected to the drying box and is threadedly engaged with the slide. The screw rod extends along the length direction of the heating chamber, and the slide slides along the axial direction of the screw rod and is engaged with the connecting box.

[0016] Optionally, the opening and closing unit includes a reduction motor and an opening and closing door, the reduction motor is provided on the drying box, the opening and closing door is rotatably connected to the drying box via a rotating shaft, and the output shaft of the reduction motor is coaxially connected to the rotating shaft;

[0017] The upper surface of the opening and closing door is slidably fitted with a scraper. When the opening and closing door is closed at the discharge port, the scraper will be located outside the drying box; when the opening and closing door is flipped downward, the scraper will move downward to the lowest point of the opening and closing door due to its own gravity.

[0018] Optionally, a driving gear is coaxially connected to the rotating shaft, a movable rack is meshed on the driving gear, the movable rack is horizontally arranged and slides along its own length to fit in the drying box, and the movable rack is connected to the scraper through a pull rope, and the drying box is provided with a plurality of guide wheels for the pull rope to pass around;

[0019] When the opening and closing door is closed at the discharge port, the scraper will be located at the rotating shaft and the pull rope will be in a taut state;

[0020] When the door is turned downward, the door will cause the driving gear to drive the moving rack to slide through the rotating shaft, so that the pull rope is in a relaxed state.

[0021] In a second aspect, the present application provides a method for removing histamine from apramycin sulfate, which adopts the following technical solution: A method for removing histamine from apramycin sulfate comprises the following steps:

[0022] S1. Selecting apramycin sulfate fermentation broth with a high histamine content, removing bacterial residues and insoluble impurities by centrifugation to obtain a centrifuge;

[0023] S2. Using a weakly acidic cation exchange resin at pH 6.5-7.0 to adsorb histamine in the centrifuge solution, and gradient eluting with a 0.1-0.5 M NaCl solution to separate the histamine from apramycin sulfate to obtain a separated solution;

[0024] S3. Adjust the pH value of the separated liquid to 10-11, extract with n-butanol or ethyl acetate, and concentrate to obtain crude histamine;

[0025] S4. Concentrate the crude histamine, adjust the pH to 3.0-4.0, and cool to 4°C for crystallization for 12 hours;

[0026] S5. Drying the crystals using the histamine removal and extraction device for apramycin sulfate according to any one of claims 1 to 9.

[0027] In summary, this application has the following beneficial technical effects:

[0028] 1. The crystals are distributed into various heating chambers. The heating plates heat the crystals in the heating chambers. The crystals in the heating chambers are continuously turned in multiple directions to avoid uneven heating of the crystals. The dried crystals are discharged through the discharge port. The jet unit blows downwards the crystals on the wall of the heating chamber to avoid adhesion. This eliminates the need for manual cleaning by workers, thereby improving the drying efficiency of the crystals.

[0029] 2. The spiral blade can transport the crystals in the heating chamber in a vertical spiral direction while moving linearly, and the crystals attached to the wall of the heating chamber will be scraped off by the spiral blade to avoid uneven heating of the crystals, thereby improving the drying effect of the crystals;

[0030] 3. When the spiral blade moves to the end of the heating chamber, the spiral blade can rotate into the concave cavity, avoiding the stirring dead angle at the end of the heating chamber, thereby further improving the drying effect of the crystals;

[0031] 4. During the unloading process of the crystals, the crystals in the heating chamber, the crystals on the spiral blades and the crystals on the opening and closing door can all be cleaned without the need for manual cleaning by workers, thereby improving the drying efficiency of the crystals. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 It is a schematic diagram of the overall structure of the embodiment of the present application;

[0033] Figure 2 This is a schematic diagram of the overall cross-sectional structure of an embodiment of the present application;

[0034] Figure 3 This is a schematic diagram of the structure inside the fixed shell of an embodiment of the present application;

[0035] Figure 4 This is a schematic diagram of the internal structure of the drying box and the connection box in the embodiment of the present application;

[0036] Figure 5 This is a schematic structural diagram of the concave cavity and the heating cavity in an embodiment of the present application;

[0037] Figure 6 Schematic diagram of the structure of the spiral conveying unit of the embodiment of the present application;

[0038] Figure 7 Schematic diagram of the cross-sectional structure of the fixed shell and the cover plate of the embodiment of the present application;

[0039] Figure 8 This is a structural diagram of the opening and closing unit of an embodiment of the present application;

[0040] Figure 9 This is a structural diagram of an opening and closing door in an embodiment of the present application.

[0041] Reference numerals: 1, drying oven; 11, feeding port; 12, feeding hopper; 121, dividing plate; 13, discharging port; 14, vacuum pump; 15, fixed shell; 16, cover plate; 17, blowing port; 2, opening and closing unit; 21, reduction motor; 22, rotating shaft; 23, opening and closing door; 231, chute; 24, scraper; 241, dovetail block; 25, driving gear; 26, moving rack; 27, guide rail; 28, pull rope; 29, guide wheel; 3, Power supply; 31. Heating plate; 32. Heating chamber; 33. Concave chamber; 4. Connecting box; 41. Slide plate; 42. Limit rod; 43. Fixed rack; 44. Transmission gear set; 5. Driving unit; 51. Driving motor; 52. Screw; 6. Screw conveying unit; 61. Spiral blade; 62. Turntable; 7. Jet unit; 71. First air pump; 72. Jet hole; 8. Blowing unit; 81. Second air pump; 9. Controller; 91. Control button. DETAILED DESCRIPTION

[0042] The following is combined with Figure 1-9 This application is described in further detail.

[0043] Example 1

[0044] The present application discloses a histamine removal and extraction device for apramycin sulfate. Figure 1 and Figure 2 As shown, a histamine removal and extraction device for apramycin sulfate includes a drying box 1, a feed port 11 is provided at the top of the drying box 1, and a discharge port 13 is provided at the bottom of the drying box 1.

[0045] A feed hopper 12 is mounted at the feed port 11. Inside the feed hopper 12 are several separators 121, each of which has a different inclination angle. Once the crystals are loaded into the feed hopper 12, the separators 121 separate the crystals so that they can be dispersed and fed into the drying oven 1.

[0046] It is worth noting that an openable and closable sealing plate may be provided on the top of the feed hopper 12 . When the crystals are dried in the drying oven 1 , the sealing plate may seal the top of the feed hopper 12 to reduce heat loss in the drying oven 1 .

[0047] A vacuum pump 14 is installed on the side of the drying box 1. The air inlet pipe of the vacuum pump 14 is connected to the interior of the drying box 1. The vacuum pump 14 can extract the gas in the drying box 1, making the interior of the drying box 1 hollow, so as to achieve vacuum drying of the crystals.

[0048] like Figure 2 and Figure 3As shown, the discharge port 13 is provided with an opening and closing unit 2 for controlling the opening and closing of the discharge port 13. The opening and closing unit 2 includes a reduction motor 21 and an opening and closing door 23. In this embodiment, two reduction motors 21 are provided, and two opening and closing doors 23 are provided. The two opening and closing doors 23 are arranged as double doors, and the reduction motors 21 correspond to the opening and closing doors 23 one by one. The reduction motor 21 is mounted on the drying oven 1, and the opening and closing doors 23 are rotatably connected to the drying oven 1 via a rotating shaft 22. The rotating shaft 22 is arranged horizontally, and the output shaft of the reduction motor 21 is coaxially connected to the rotating shaft 22. The reduction motor 21 can drive the opening and closing doors 23 to flip up and down via the rotating shaft 22, thereby realizing the opening and closing of the discharge port 13.

[0049] A number of heating plates 31 are mounted on the inner wall of the drying box 1 and arranged in sequence along the horizontal direction. Every two adjacent heating plates 31 together form a heating chamber 32. The crystals separated by the separating plate 121 will fall into each heating chamber 32. The interior of the heating plate 31 is hollow and is equipped with heating wires. A power supply 3 is mounted on the outer wall of the drying box 1. The power supply 3 is connected to all the heating wires through cables, thereby supplying power to the heating wires. After receiving power, the heating wires will transfer heat to the heating plate 31, so that the crystals in the heating chamber 32 are heated and dried.

[0050] like Figures 3 to 5 As shown, the left and right sides of the drying box 1 are connected to the connecting box 4, and a slide 41 and a driving unit 5 are provided in the drying box 1. The two ends of the slide 41 extend into the two connecting boxes 4 respectively. A limit rod 42 is provided in the connecting box 4, and the limit rod 42 extends along the length direction of the heating chamber 32. Both ends of the limit rod 42 are installed on the inner wall of the connecting box 4. The limit rod 42 slides through the end of the slide 41. The two limit rods 42 jointly limit the slide 41 so that the slide 41 can only move along the length direction of the heating chamber 32.

[0051] The drive unit 5 includes a drive motor 51 mounted on the outer wall of the drying chamber 1. A screw rod 52 is coaxially connected to the output shaft of the drive motor 51. The screw rod 52 rotates about its own axis and is connected to the drying chamber 1. It is threadedly engaged with the slide plate 41 and extends along the length of the heating chamber 32. When the drive motor 51 drives the screw rod 52 to rotate, the screw rod 52 drives the slide plate 41 to move along the length of the heating chamber 32.

[0052] like Figure 4 and Figure 6 As shown, a plurality of vertically arranged spiral conveying units 6 are provided on the slide 41. The spiral conveying units 6 correspond to the heating chambers 32 one by one and extend into the corresponding heating chambers 32. The spiral conveying units 6 include vertically arranged spiral leaves 61. The spiral leaves 61 are in contact with the left and right side walls of the heating chamber 32. The spiral leaves 61 rotate around their own axes and are connected to the slide 41. Every two adjacent spiral leaves 61 are connected by a belt drive, so that all the spiral leaves 61 can rotate synchronously in the same direction.

[0053] like Figure 4 and Figure 5 As shown, a fixed rack 43 is installed on the inner wall of the connecting box 4, and the length direction of the fixed rack 43 is the same as the length direction of the heating chamber 32; both ends of the slide 41 are rotatably connected to a transmission gear set 44, and the transmission gear set 44 is composed of a plurality of gears. The input end of the transmission gear set 44 is engaged with the fixed rack 43, and the output end of the transmission gear set 44 is coaxially connected to the spiral leaf 61.

[0054] When the driving motor 51 drives the screw rod 52 to rotate, causing the slide plate 41 to move along the length direction of the heating chamber 32, the slide plate 41 will drive the transmission gear set 44 to move on the fixed rack 43, and the fixed rack 43 will drive the spiral blade 61 to rotate through the transmission gear set 44, so that the spiral blade 61 can move linearly while spirally transporting the crystals in the heating chamber 32 in the vertical direction. Therefore, the crystals in the heating chamber 32 will be continuously turned over in multiple directions, and the crystals attached to the wall of the heating chamber 32 will be scraped off by the spiral blade 61 to avoid uneven heating of the crystals, thereby improving the drying effect of the crystals.

[0055] like Figure 5 As shown, concave cavities 33 are provided at the front and rear ends of the heating chamber 32. The cross-section of the concave cavity 33 is semicircular and is for the spiral blade 61 to rotate and embed. Therefore, when the spiral blade 61 moves to the end of the heating chamber 32, the spiral blade 61 can rotate into the concave cavity 33. The setting of the concave cavity 33 avoids the occurrence of a stirring dead angle at the end of the heating chamber 32, thereby further improving the drying effect of the crystals.

[0056] It is worth noting that the upper part of the spiral blade 61 protrudes outside the heating chamber 32, so the spiral blade 61 can spirally transport the crystals in the heating chamber 32 upward to the outside of the heating chamber 32, that is, the crystals in each heating chamber 32 can fall into the adjacent heating chamber 32; in addition, in a heating chamber 32 with more crystals, the overall height of the crystals is higher, and more crystals are discharged by the spiral blade 61. Therefore, as the spiral blade 61 moves back and forth, the amount of crystals in multiple heating chambers 32 will be more evenly distributed, avoiding the situation where there are too many or too few crystals in the heating chamber 32, thereby ensuring the drying efficiency of the heating chamber 32 for the crystals.

[0057] A turntable 62 is integrally formed at the lower end of the spiral blade 61. The sidewalls of the turntable 62 contact the left and right walls of the heating chamber 32, and the lower surface of the turntable 62 contacts the opening and closing door 23 that seals the discharge port 13. As the spiral blade 61 rotates, the turntable 62 rotates with the spiral blade 61 and scrapes away crystals attached to the lower wall of the heating chamber 32 and the upper surface of the opening and closing door 23. This prevents some crystals from clinging to the heating chamber 32 for a long time and affecting the drying of the remaining crystals in the heating chamber 32, thereby improving the drying efficiency of the crystals.

[0058] When the crystals are dried, the reduction motor 21 will drive the opening and closing doors 23 to flip downward through the rotating shaft 22, so that the two opening and closing doors 23 are opened, and the dried crystals will be discharged through the discharge port 13; workers can place a collection box under the two opening and closing doors 23 to collect the dried crystals.

[0059] like Figure 4 and Figure 6 As shown, a plurality of jet units 7 are provided on the slide 41, and each two groups of jet units 7 correspond to a heating chamber 32. The interior of the slide 41 is hollow. The jet unit 7 includes a first air pump 71 and a plurality of jet holes 72 vertically facing downward and connected to the interior of the slide 41. The first air pump 71 is installed on the outer wall of the drying box 1. The first air pump 71 is connected to the interior of the slide 41 through a hose, and the jet holes 72 are provided on the lower surface of the slide 41.

[0060] During the crystal feeding process, the spiral blade 61 will still move back and forth in the heating chamber 32. At this time, the first air pump 71 will start and introduce gas into the inside of the slide 41 through the hose. The gas in the soft plate will be ejected vertically downward through the jet hole 72 to the wall of the heating chamber 32, so that the residual crystals attached to the wall of the heating chamber 32 can be blown out of the heating chamber 32.

[0061] like Figure 4 and Figure 8 As shown, the drying box 1 includes a fixed shell 15, the side of the fixed shell 15 is open and welded with a cover plate 16, the cover plate 16 is welded by multiple steel plates, so that the interior of the cover plate 16 is hollow; the concave cavity 33 at one end of the heating chamber 32 is provided on the fixed shell 15, and the concave cavity 33 at the other end of the heating chamber 32 is provided on the cover plate 16, and the cover plate 16 is provided with a plurality of downwardly inclined blowing ports 17 on the side facing the fixed shell 15, the blowing ports 17 are connected to the interior of the cover plate 16, and the blowing ports 17 are arranged in sequence along the vertical direction; the drying box 1 is provided with a blowing unit 8, the blowing unit 8 includes a second air pump 81 installed on the outer wall of the cover plate 16, and the second air pump 81 is connected to the interior of the cover plate 16 through a hose.

[0062] During the crystal feeding process, when the spiral blade 61 moves to the concave cavity 33 of the cover plate 16, the second air pump 81 will start and introduce gas into the inside of the cover plate 16 through the hose. The gas inside the cover plate 16 will be sprayed toward the rotating spiral blade 61 through the blowing port 17, so that the crystals attached to the spiral blade 61 can be blown away.

[0063] like Figure 8 and Figure 9 As shown, a scraper 24 is slidably fitted on the upper surface of the opening and closing door 23, and two dovetail blocks 241 are installed on the lower surface of the scraper 24. Two slide grooves 231 are provided on the upper surface of the opening and closing door 23, and the dovetail blocks 241 are slidably embedded in the slide grooves 231, thereby realizing the sliding fit between the scraper 24 and the opening and closing door 23.

[0064] It is worth noting that the total length of the two opening and closing doors 23 is greater than the length of the discharge port 13, and the width of the opening and closing doors 23 is greater than the width of the discharge port 13. When the opening and closing doors 23 are closed at the discharge port 13, the scraper 24 will be located at the rotating shaft 22 and outside the drying box 1; during the process of the opening and closing doors 23 turning downward, the crystals in the heating chamber 32 will be discharged through the discharge port 13; after the crystals in the heating chamber 32 are discharged, the opening and closing doors 23 have rotated to the specified inclination. At this time, the downward force generated by the scraper 24's own weight will be greater than the static friction force of the opening and closing doors 23 on the scraper 24. Therefore, the scraper 24 will slide down the upper surface of the opening and closing doors 23 and continue to slide to the lowest point of the opening and closing doors 23, so that all crystals attached to the upper surface of the opening and closing doors 23 are scraped off.

[0065] Therefore, during the crystal unloading process, the crystals in the heating chamber 32, the crystals on the spiral blades 61 and the crystals on the opening and closing door 23 can all be cleaned without manual cleaning by workers, thereby improving the drying efficiency of the crystals.

[0066] A driving gear 25 is coaxially connected to the rotating shaft 22, and a movable rack 26 is engaged with the driving gear 25. The movable rack 26 is arranged horizontally. A guide rail 27 is installed on the drying box 1. The length direction of the guide rail 27 is the same as the length direction of the movable rack 26. The movable rack 26 slides along its own length direction to fit the guide rail 27 on the drying box 1, and the movable rack 26 is connected to the scraper 24 through a pull rope 28. A plurality of guide wheels 29 are rotatably connected to the drying box 1 for the pull rope 28 to pass around.

[0067] When the opening and closing door 23 is closed at the discharge port 13, the scraper 24 will be located at the rotating shaft 22 and on the outside of the drying box 1, and the pull rope 28 will be in a taut state; in the process of the opening and closing door 23 flipping downward, the opening and closing door 23 will drive the driving gear 25 to rotate through the rotating shaft 22, and the driving gear 25 will drive the moving rack 26 to slide, so that the pull rope 28 is in a relaxed state; after the opening and closing door 23 has rotated to the specified inclination, the scraper 24 will slide down due to its own gravity to clean the upper surface of the opening and closing door 23, so that the pull rope 28 is tightened again; in the process of the reduction motor 21 driving the opening and closing door 23 to flip and reset upward, the opening and closing door 23 will drive the driving gear 25 to rotate through the rotating shaft 22, and the driving gear 25 will drive the moving rack 26 to move and reset, and the moving rack 26 will pull the scraper 24 to move through the pull rope 28, so that the scraper 24 moves and resets, so that the scraper 24 can continue to clean the crystals in the subsequent process.

[0068] like Figure 1 As shown, a controller 9 is installed on the outer wall of the drying box 1, and the power supply 3, drive motor 51, first air pump 71, second air pump 81 and reduction motor 21 are all coupled to the controller 9; three control buttons 91 are provided on the controller 9, the first control button 91 is a drying button, pressing the control button 91 can control the power supply 3 and drive motor 51 to turn on, so that the spiral blade 61 can spirally transport the crystals; the second control button 91 is a unloading button, pressing the control button 91 can control the first air pump 71 and the second air pump 81 to turn on and control the reduction motor 21 to drive the opening and closing door 23 to turn down to a specified inclination through the rotating shaft 22, so that the crystals in the heating chamber 32 can be discharged into the collection box through the discharge port 13; the third control button 91 is a stop button, pressing the button can control the reduction motor 21 to drive the opening and closing door 23 to flip and reset through the rotating shaft 22 and control the power supply 3, drive motor 51, first air pump 71 and second air pump 81 to turn off, so that the drying box 1 can continue to dry next time.

[0069] The implementation principle of the histamine removal and extraction equipment of apramycin sulfate in the embodiment of the present application is as follows: during the drying process of the crystals, the crystals will be loaded into the feed hopper 12, and the distribution plate 121 will distribute the crystals into each heating chamber 32; then the worker will seal the top of the feed hopper 12 through the sealing plate, and evacuate the inside of the drying box 1 to a vacuum state through the vacuum pump 14.

[0070] After that, the worker presses the first control button 91, and the controller 9 will control the power supply 3 and the drive motor 51 to turn on. The power supply 3 will supply power to the heating plate 31, so that the heating plate 31 heats the crystals in the heating chamber 32. The drive motor 51 will drive the screw rod 52 to rotate, and the screw rod 52 will drive the slide 41 to slide along the length direction of the heating chamber 32, so that the spiral blade 61 spirally transports the crystals in the heating chamber 32. The crystals in the heating chamber 32 will be continuously flipped in multiple directions to avoid uneven heating of the crystals.

[0071] When the crystals are dried, the worker presses the second button, the controller 9 controls the first air pump 71 and the second air pump 81 to start and controls the reduction motor 21 to drive the opening and closing door 23 to turn down to the specified inclination through the rotating shaft 22, and the crystals in the heating chamber 32 are discharged into the collection box through the discharge port 13; the first air pump 71 introduces gas into the slide 41 through the hose, and the gas in the soft board is ejected vertically downward to the cavity wall of the heating chamber 32 through the jet hole 72, so that the crystals attached to the cavity of the heating chamber 32 are discharged into the collection box through the discharge port 13; the first air pump 71 injects gas into the slide 41 through the hose, and the gas in the soft board is ejected vertically downward to the cavity wall of the heating chamber 32 through the jet hole 72, so that the crystals attached to the cavity of the heating chamber 32 are discharged vertically. The residual crystals on the wall are blown out of the heating chamber 32; the second air pump 81 will introduce gas into the inside of the cover plate 16 through the hose, and the gas inside the cover plate 16 will be sprayed toward the rotating spiral blade 61 through the blowing port 17, so that the crystals attached to the spiral blade 61 are blown away; after the crystals in the heating chamber 32 are discharged, the opening and closing door 23 has rotated to the specified inclination, and the scraper 24 will slide down to the lowest point of the opening and closing door 23 due to its own gravity, so that all the crystals attached to the upper surface of the opening and closing door 23 are scraped off.

[0072] Finally, the worker will press the third button, and the controller 9 will control the reduction motor 21 to drive the opening and closing door 23 to flip and reset through the rotating shaft 22 and control the power supply 3, the drive motor 51, the first air pump 71 and the second air pump 81 to turn off. The opening and closing door 23 will drive the movable rack 26 to move through the drive gear 25, and the movable rack 26 will pull the scraper 24 to move and reset through the pull rope 28. The opening and closing door 23 will be re-closed at the discharge port 13, so that the drying box 1 can continue to dry next time.

[0073] In summary, the present application can distribute the crystals into each heating chamber 32, thereby increasing the heating area of the crystals; the crystals can be continuously turned in multiple directions during the drying process, so that the crystals in the heating chamber 32 can be heated evenly; the crystals will be cleaned in multiple aspects during the unloading process to avoid residue, thereby improving the drying efficiency of the crystals.

[0074] Example 2

[0075] A method for removing histamine from apramycin sulfate comprises the following steps:

[0076] S1. Select apramycin sulfate fermentation broth with a high histamine content (the histamine concentration must be confirmed by HPLC), remove bacterial residues and insoluble impurities by centrifugation to obtain a centrifuge, and centrifuge at a speed of 3000-5000 rpm for 10-15 min;

[0077] S2. Using a weakly acidic cation exchange resin (such as D151 or D152) to adsorb histamine (histamine pKa ≈ 5.7, present in a cationic form) in the centrifuge solution at pH 6.5-7.0, and gradient eluting with a 0.1-0.5 M NaCl solution to separate the histamine from apramycin sulfate (which contains multiple amino groups and has stronger adsorption), to obtain a separated solution;

[0078] S3. Adjust the pH value of the separated solution to 10-11 (histamine is deprotonated and converted to a free state), extract with n-butanol or ethyl acetate, and concentrate to obtain crude histamine;

[0079] S4. Concentrate the crude histamine to an appropriate concentration, adjust the pH to 3.0-4.0, and cool to 4°C for crystallization for 12 hours at a cooling rate of 0.5°C / min.

[0080] S5. Drying the crystals using the above-mentioned apramycin sulfate histamine removal and extraction device.

[0081] The above are all preferred embodiments of the present application, and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.

Claims

1. A histamine removal and extraction device for apramycin sulfate, characterized by: The drying box (1) comprises a feeding port (11) and a discharging port (13), wherein an opening and closing unit (2) for controlling the opening and closing of the discharging port (13) is provided at the discharging port (13); a plurality of heating plates (31) arranged in sequence in a horizontal direction are provided in the drying box (1), and every two adjacent heating plates (31) together form a heating chamber (32); A slide plate (41) and a driving unit (5) for driving the slide plate (41) to move along the length direction of the heating chamber (32) are provided in the drying box (1); a plurality of jet units (7) and a plurality of vertically arranged spiral conveying units (6) are provided on the slide plate (41); each two groups of jet units (7) correspond to one heating chamber (32) and blow vertically toward the left and right side walls of the heating chamber (32), respectively; the spiral conveying units (6) correspond to the heating chambers (32) one by one and extend into the corresponding heating chambers (32).

2. The histamine removal and extraction equipment for apramycin sulfate according to claim 1, characterized in that: The spiral conveying unit (6) comprises a vertically arranged spiral blade (61), which rotates around its own axis and is connected to the slide plate (41), and every two adjacent spiral blades (61) are connected through a belt transmission.

3. The histamine removal and extraction equipment for apramycin sulfate according to claim 2, characterized in that: The side of the drying box (1) is connected to a connecting box (4), and a fixed rack (43) is provided in the connecting box (4), and the length direction of the fixed rack (43) is the same as the length direction of the heating chamber (32); A transmission gear set (44) is provided on the slide plate (41), the transmission gear set (44) is in transmission connection with one of the spiral leaves (61) located at the edge, and the transmission gear set (44) is engaged with the fixed rack (43).

4. The histamine removal and extraction equipment for apramycin sulfate according to claim 2, characterized in that: A turntable (62) is provided on the spiral blade (61), the side walls of the turntable (62) abut against the left and right walls of the heating chamber (32), and the lower surface of the turntable (62) abuts against the opening and closing unit (2) that closes the discharge port (13).

5. The histamine removal and extraction equipment for apramycin sulfate according to claim 2, characterized in that: The heating chamber (32) is provided with concave cavities (33) at both the front and rear ends. The cross section of the concave cavity (33) is semicircular and is used for the spiral blade (61) to be rotatably embedded.

6. The histamine removal and extraction equipment for apramycin sulfate according to claim 5, characterized in that: A plurality of downwardly inclined air blowing ports (17) are provided on the wall of one of the concave cavities (33), and an air blowing unit (8) connected to all the air blowing ports (17) is provided on the drying box (1).

7. The histamine removal and extraction equipment for apramycin sulfate according to claim 3, characterized in that: The driving unit (5) includes a driving motor (51) provided on the drying box (1), a screw rod (52) being coaxially connected to the output shaft of the driving motor (51), the screw rod (52) being connected to the drying box (1) by rotating around its own axis and being threadedly engaged with the slide plate (41), the screw rod (52) extending along the length direction of the heating chamber (32), and the slide plate (41) being axially slidably engaged with the connecting box (4) along the screw rod (52).

8. The histamine removal and extraction equipment for apramycin sulfate according to claim 1, characterized in that: The opening and closing unit (2) comprises a reduction motor (21) and an opening and closing door (23); the reduction motor (21) is arranged on the drying box (1); the opening and closing door (23) is rotatably connected to the drying box (1) via a rotating shaft (22); and the output shaft of the reduction motor (21) is coaxially connected to the rotating shaft (22); The upper surface of the opening and closing door (23) is slidably fitted with a scraper (24). When the opening and closing door (23) is closed at the discharge port (13), the scraper (24) will be located outside the drying box (1); when the opening and closing door (23) is turned downward, the scraper (24) will move downward to the lowest point of the opening and closing door (23) due to its own gravity.

9. The histamine removal and extraction equipment for apramycin sulfate according to claim 8, characterized in that: A driving gear (25) is coaxially connected to the rotating shaft (22), and a movable rack (26) is meshed with the driving gear (25). The movable rack (26) is horizontally arranged and slides along its own length direction to fit in the drying box (1). The movable rack (26) is connected to the scraper (24) through a pull rope (28). The drying box (1) is provided with a plurality of guide wheels (29) for the pull rope (28) to pass around. When the opening and closing door (23) is closed at the discharge port (13), the scraper (24) will be located at the rotating shaft (22) and the pull rope (28) will be in a taut state; When the opening and closing door (23) is turned downward, the opening and closing door (23) will cause the driving gear (25) to drive the moving rack (26) to slide through the rotating shaft (22), so that the pull rope (28) is in a relaxed state.

10. A method for removing histamine from apramycin sulfate, characterized in that: The following steps are included: S1. Selecting apramycin sulfate fermentation broth with a high histamine content, removing bacterial residues and insoluble impurities by centrifugation to obtain a centrifuge; S2. Using a weakly acidic cation exchange resin at pH 6.5-7.0 to adsorb histamine in the centrifuge solution, and gradient eluting with a 0.1-0.5 M NaCl solution to separate the histamine from apramycin sulfate to obtain a separated solution; S3. Adjust the pH value of the separated liquid to 10-11, extract with n-butanol or ethyl acetate, and concentrate to obtain crude histamine; S4. Concentrate the crude histamine, adjust the pH to 3.0-4.0, and cool to 4°C for crystallization for 12 hours; S5. Drying the crystals using the histamine removal and extraction device for apramycin sulfate according to any one of claims 1 to 9.

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