Strawberry seedling hardening method of tissue culture seedling rooting and seedling hardening cultivation frame in plant factory

Abstract

The invention belongs to the technical field of strawberry cultivation, and discloses a strawberry seedling hardening method of a tissue culture seedling rooting and seedling hardening cultivation frame in a plant factory, which comprises the following steps: cultivating strawberry seedlings at room temperature for 6-7 days, moving the seedlings out, soaking the seedlings in a carbendazim solution for 2-3 minutes, then cleaning, smearing seedling roots with a rooting solution, disinfecting a substrate, moving the seedlings out of a culture medium, and transplanting the seedlings into a seedling culture room. The method comprises the following steps: cleaning a substrate, wrapping the substrate, putting the substrate into a plug tray for culture, transplanting seedlings, putting the transplanted seedlings into a seedling hardening frame, keeping preset temperature, humidity and illumination, culturing for 20-35 days for rooting, removing a heat insulation plate after rooting, adjusting the preset temperature, humidity and illumination of 7000-8000lux, and hardening the seedlings for one week. According to the method, the heating energy cost can be saved by more than or equal to 10%, the water can be saved by more than or equal to 10%, compared with a common seedling hardening process, the rooting and seedling hardening operation can be completed within 6 weeks, in addition, the loss rate of strawberry seedlings in the rooting and seedling hardening period can be effectively reduced, and the predicted seedling rate is more than or equal to 90%.

Description

Technical Field

[0001] The invention belongs to the technical field of strawberry cultivation, and particularly relates to a strawberry seedling hardening method using a tissue culture seedling rooting and hardening cultivation rack in a plant factory. Background Art

[0002] With the rapid development of modern agricultural technology, plant factories have garnered widespread attention as an efficient, environmentally friendly, and sustainable agricultural production model. Within plant factories, strawberry tissue culture has become a key research area due to its high propagation efficiency, short growth cycle, and excellent quality. However, traditional strawberry tissue culture methods generally involve the following: mother plant cultivation, virus testing, explant induction, multiplication and propagation, seedling rooting, and hardening. These methods suffer from drawbacks such as long production cycles and high seedling losses. This long cycle increases production time, costs, and risks. Furthermore, the high humidity and temperature in greenhouse environments can easily lead to pests and diseases, increasing seedling losses and negatively impacting production efficiency and economic benefits.

[0003] Therefore, considering the above factors, a strawberry seedling hardening method using a tissue culture seedling rooting and hardening cultivation rack in a plant factory is proposed to solve the above problems. Summary of the Invention

[0004] The object of the present invention is to provide a strawberry seedling hardening method using a tissue culture seedling rooting and hardening cultivation rack in a plant factory to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solution: a strawberry seedling hardening method using a tissue culture seedling rooting and hardening cultivation rack in a plant factory, comprising the following steps: S1: Place the strawberry seedlings in the tissue culture bottle at room temperature for 6-7 days; S2: Remove the strawberry seedlings from the tissue culture bottle and soak them in carbendazim solution for 2-3 minutes, remove them and rinse them with clean water; S3: Dip a short brush into the rooting solution and apply it evenly on the root base of the seedlings; S4: sterilize the substrate; S5: Remove the test tube seedlings from the agar medium, clean the base thoroughly, wrap the seedlings with substrate, and place them in a plug tray for culture; S6: The transplanted seedlings are placed in a seedling cultivation rack for rooting and hardening, and all the insulation boards are installed on the hardening rack. The mist device is turned on to maintain the preset humidity and temperature inside the cultivation rack, and the preset light intensity is irradiated. The cultivation is continued for 20-35 days until the tissue culture seedlings take root; S7: After the strawberry seedlings have taken root, remove the insulation board, adjust the humidity to the preset humidity, adjust the temperature to the preset temperature, and the light to 7000lux-8000lux. After one week of cultivation, harden the seedlings.

[0006] Preferably, the S4 specifically includes the steps of: Sterilize the matrix in a 90-95°C steam bath for 20-30 minutes.

[0007] Preferably, the carbendazim solution consists of carbendazim and water in a mass ratio of 1:1000.

[0008] Preferably, the rooting liquid consists of a rooting agent and water in a mass ratio of 1:50-80.

[0009] Preferably, in S6, the preset humidity is 85%-90%, and the preset temperature is 25°C-30°C.

[0010] Preferably, in S6, the preset light intensity is 3000 lux.

[0011] Preferably, the matrix is a mixture of coconut husk and perlite.

[0012] Preferably, in the matrix, the ratio of coconut husk to perlite is 7:3.

[0013] Preferably, in S7, the preset humidity is 70%-75%, and the preset temperature includes 23°C.

[0014] Preferably, the cell tray comprises a 32-cell tray or a 50-cell tray, and the outer contours of the 32-cell tray and the 50-cell tray are both 54.6 cm*27.5 cm.

[0015] Compared with the prior art, the present invention has the following beneficial effects: The present invention can save heating costs and water resources: compared with ordinary open cultivation rack seedling hardening, it is estimated that heating energy costs can be saved by ≥10%, and water can be saved by ≥10%. In addition, compared with the ordinary seedling hardening process, the present seedling hardening method can shorten the rooting and seedling hardening time and improve the seedling hardening efficiency. Compared with the ordinary method which takes about 5-10 weeks, the present invention only needs less than 6 weeks to complete the rooting and seedling hardening operation. In addition, the present method can effectively reduce the loss rate of strawberry seedlings during the rooting and seedling hardening period, and the seedling success rate is expected to be ≥90%. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 Flowchart of the present invention. DETAILED DESCRIPTION

[0017] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0018] See also Figure 1 As shown, the present invention provides the following technical solutions: 1. Matrix Preparation In the present invention, the substrate is first selected: coconut husk and perlite are mixed in a ratio of 7:3 to form a mixture, and then the mixture is sterilized in a steam bath at a temperature of 90-95° C. for 20-30 minutes.

[0019] In addition, in the present invention, it is necessary to use a plug tray, so before hardening the seedlings, it is necessary to select the specifications of the plug tray. Regarding the plug tray model, it includes a 32-plug tray or a 50-plug tray, and the outer contours of the 32-plug tray and the 50-plug tray are both 54.6cm*27.5cm.

[0020] 2. Transplanting and hardening of strawberry tissue culture seedlings After the substrate is prepared and sterilized, the seedlings need to be adapted to room temperature. The specific process is as follows: Place the strawberry seedlings in the tissue culture bottle at room temperature for 6-7 days; The seedling removal operation is performed, and the specific process is as follows: the strawberry seedlings are removed from the tissue culture bottle and immersed in a carbendazim solution consisting of carbendazim and water at a mass ratio of 1:1000 for 2-3 minutes, removed, rinsed with clean water, and a short brush is dipped in a rooting solution consisting of a rooting agent and water at a mass ratio of 1:50-80, and evenly applied to the root base of the seedlings; Remove the test tube seedlings from the agar culture medium and carefully remove them with long tweezers to avoid damaging the test tube seedlings. Clean the base of the test tube seedlings thoroughly to prevent residual sucrose and nutrients from causing root rot and death of the seedlings. After the seedlings are taken out, wrap them with a substrate and place them in a hole tray for culture.

[0021] The specific process of rooting culture includes the following steps: the transplanted seedlings are placed in the seedling rooting and hardening cultivation rack, all the insulation boards of the hardening rack are installed, and the mist device is turned on to keep the humidity inside the cultivation rack at 85%-90%, the temperature at 25℃-30℃, the light at 3000lux, and the cultivation is continued for 20-35 days until the tissue culture seedlings take root.

[0022] Hardening of seedlings: After the strawberry seedlings have taken root, remove the insulation board, control the humidity at 70%-75%, the temperature at around 23°C, and the light at 7000lux-8000lux, and harden the seedlings after about a week.

[0023] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A strawberry seedling hardening method using a tissue culture seedling rooting and hardening cultivation rack in a plant factory, characterized by: The steps include: S1: Place the strawberry seedlings in the tissue culture bottle at room temperature for 6-7 days; S2: Remove the strawberry seedlings from the tissue culture bottle and soak them in carbendazim solution for 2-3 minutes, remove them and rinse them with clean water; S3: Dip a short brush into the rooting solution and apply it evenly on the root base of the seedlings; S4: sterilize the substrate; S5: Remove the test tube seedlings from the agar medium, clean the base thoroughly, wrap the seedlings with substrate, and place them in a plug tray for culture; S6: The transplanted seedlings are placed in a seedling cultivation rack for rooting and hardening, and all the insulation boards are installed on the hardening rack. The mist device is turned on to maintain the preset humidity and temperature inside the cultivation rack, and the preset light intensity is irradiated. The cultivation is continued for 20-35 days until the tissue culture seedlings take root; S7: After the strawberry seedlings have taken root, remove the insulation board, adjust the humidity to the preset humidity, adjust the temperature to the preset temperature, and the light to 7000lux-8000lux. After one week of cultivation, harden the seedlings.

2. The strawberry seedling hardening method of a plant factory tissue culture seedling rooting and hardening cultivation frame according to claim 1, wherein: The S4 specifically includes the steps of: Sterilize the matrix in a 90-95°C steam bath for 20-30 minutes.

3. The strawberry seedling hardening method of the tissue culture seedling rooting and hardening cultivation frame in a plant factory according to claim 1, wherein: The carbendazim solution consists of carbendazim and water in a mass ratio of 1:1000.

4. The strawberry seedling hardening method of a tissue culture seedling rooting and hardening cultivation frame in a plant factory according to claim 1, wherein: The rooting liquid consists of a rooting agent and water in a mass ratio of 1:50-80.

5. The strawberry seedling hardening method of a plant factory tissue culture seedling rooting and hardening cultivation frame according to claim 1, wherein: In the step S6, the preset humidity is 85%-90%, and the preset temperature is 25°C-30°C.

6. The strawberry seedling hardening method of a plant factory tissue culture seedling rooting and hardening cultivation frame according to claim 1, wherein: In the above-mentioned S6, the preset light intensity is 3000 lux.

7. The strawberry seedling hardening method of a plant factory tissue culture seedling rooting and hardening cultivation frame according to claim 1, wherein: The matrix is a mixture of coconut husk and perlite.

8. The strawberry seedling hardening method of a plant factory tissue culture seedling rooting and hardening cultivation rack according to claim 7, wherein: In the matrix, the ratio of coconut husk to perlite is 7:

3.

9. The strawberry seedling hardening method of a plant factory tissue culture seedling rooting and hardening cultivation frame according to claim 1, wherein: In the step S7, the preset humidity is 70%-75%, and the preset temperature includes 23°C.

10. The strawberry seedling hardening method using a tissue culture seedling rooting and hardening cultivation rack in a plant factory according to claim 1, wherein: The cell tray includes a 32-cell tray or a 50-cell tray, and the outer contours of the 32-cell tray and the 50-cell tray are both 54.6 cm*27.5 cm.

Images