Indoor algae-shellfish symbiosis artificial culture system and method

Abstract

The invention discloses an indoor algal-shellfish symbiosis artificial culture system and method. The system comprises a culture box with a box cover, an adhesion matrix device and a water environment control system, a partition plate is vertically arranged in the incubator, so that a water flow channel is formed between the partition plate and the inner wall of the incubator, and two functional areas, namely a first area and a second area, are further formed on the two sides of the partition plate; the substrate attaching device is arranged in the second area; the water environment control system comprises a flow speed regulation and control unit, an illumination unit, a heating unit, an oxygen dissolving unit and a control unit, and the flow speed regulation and control unit, the illumination unit, the heating unit and the oxygen dissolving unit are all distributed in the first area and controlled by the control unit. The method comprises the following steps: adding shellfish onto an adhesion substrate device; adding algae into the second area; the water environment control system is used for regulating and controlling the water body flow velocity, illumination intensity, water body temperature and water body dissolved oxygen. The method is applied to indoor artificial propagation of the limnoperna fortunei, and the artificial breeding survival rate of the limnoperna fortunei can be increased.

Description

Technical Field

[0001] The present inventor relates to the technical field of artificial cultivation of freshwater shellfish, and in particular to an indoor algae-shellfish symbiotic artificial cultivation system and method. Background Art

[0002] Freshwater molluscs, scientifically known as the slug, belong to the bivalve order Mytilidae and are a common fouling organism in inter-basin water diversion projects. On the one hand, the large-scale attachment and reproduction of freshwater molluscs in water pipes and other locations can lead to blockages, thereby reducing water transfer capacity. Furthermore, the attachment of freshwater molluscs to pipe walls can damage them, accelerating corrosion and seriously threatening the structural safety of water conservancy projects. On the other hand, as invasive species, freshwater molluscs can spread to new water bodies through inter-basin water diversion projects, disrupting the balance of ecosystems. Clearly, research on freshwater molluscs is necessary.

[0003] In the related art, research on freshwater kelp is mostly carried out through field observation experiments in different regions, or indoor experiments simulating freshwater kelp habitats. Among them, field experiments are costly and difficult to study, and indoor experiments are also difficult for freshwater kelp to survive for a long time indoors because freshwater kelp requires a high control environment for growth and reproduction.

[0004] Therefore, how to make freshwater shellfish survive for a long time indoors to grow and reproduce, and lay the foundation for the basic ecology and environmental tolerance research of freshwater shellfish, is also the top priority of the present invention. Summary of the Invention

[0005] In order to solve or partially solve the problems existing in the related art, the present invention provides an indoor algae-shell symbiotic artificial cultivation system and method, which can effectively improve the artificial cultivation survival rate of freshwater shellfish and realize the indoor artificial reproduction of freshwater shellfish.

[0006] A first aspect of the present invention provides an indoor algae-shellfish symbiotic artificial cultivation system, comprising an incubator with a lid, an attachment substrate device, and a water environment control system; a partition plate is vertically provided in the incubator to form a water flow channel between the partition plate and the inner wall of the incubator, and two functional areas are formed on both sides of the partition plate: a first area and a second area;

[0007] The attachment matrix device is arranged in the second area; the water environment control system includes a flow rate control unit, a lighting unit, a heating unit, a dissolved oxygen unit and a control unit, wherein the flow rate control unit, the lighting unit, the heating unit and the dissolved oxygen unit are all arranged in the first area and are all controlled by the control unit;

[0008] The flow rate control unit includes: a water-stop baffle erected in the first area, one end of which is connected to the partition plate and the other end is connected to the inner wall of the incubator; a submersible pump, which is located on the water inlet side of the water-stop baffle, and is connected to the hole provided on the water-stop baffle through an outlet pipe or the outlet pipe passes through the hole; when the submersible pump is started, the water on the water inlet side of the water-stop baffle flows out from the water outlet side of the water-stop baffle through the outlet pipe, so that the water contained in the incubator circulates along the water flow channel;

[0009] Among them, the illumination unit, the heating unit and the dissolved oxygen unit are all arranged on the water outlet side of the water-stop baffle.

[0010] In an optional embodiment, the cross-section of the incubator is racetrack-shaped or elliptical.

[0011] In an optional embodiment, the partition plate is arranged along the long axis direction of the cross section of the incubator.

[0012] In an optional embodiment, a plurality of channels are provided on the water-stop baffle, the channels are positioner channels, and the positioner channels are provided with a switch valve, which controls the conduction or isolation of the positioner channels; the water outlet pipe passes through one of the positioner channels.

[0013] In an optional embodiment, the lighting unit includes a light source suspended above the second area.

[0014] In an optional embodiment, the attachment matrix device includes a frame and at least one type of attachment material: the frame is provided at the bottom of the second area; and the attachment material is laid in the frame.

[0015] In an optional embodiment, a flow stabilizing grid plate and a trash grille plate are vertically provided in the incubator, and the flow stabilizing grid plate and the trash grille plate are respectively arranged at both ends of the partition plate; wherein, the trash grille plate and the water-stop partition plate are located on the same side of the partition plate, one end of the trash grille plate is connected to the first end of the partition plate, and the other end thereof is connected to the inner wall of the incubator; the submersible pump is located between the trash grille plate and the water-stop partition plate; the flow stabilizing grid plate and the attachment matrix device are located on the same side of the partition plate, one end of the flow stabilizing grid plate is connected to the second end of the partition plate, and the other end thereof is connected to the inner wall of the incubator.

[0016] In an optional embodiment, a sewage interception trough is provided at the bottom of the incubator, and the sewage interception trough is provided on the extension line of the partition plate and close to the water inlet side of the water-stop partition; a filter port is provided at the bottom of the sewage interception trough.

[0017] A second aspect of the present invention provides an indoor algae-shellfish symbiotic artificial cultivation method, which uses the above-mentioned indoor algae-shellfish symbiotic artificial cultivation system for artificial cultivation, comprising:

[0018] Fill the incubator with water;

[0019] Adding shellfish to the attachment substrate device;

[0020] adding algae to the second area on the outlet side of the water stop baffle;

[0021] Use the flow rate control unit, lighting unit, heating unit, and dissolved oxygen unit to control the water flow rate, light intensity, water temperature, and dissolved oxygen in the water to reach the preset values;

[0022] Close the lid of the box.

[0023] In an optional embodiment, the density of the shellfish is 900 / m 2 -1100 pieces / m 2 ; and the algae cultivation density in the incubator is controlled to be 3×10 5 -5×10 5 cell / L.

[0024] The technical solution provided by the present invention can have the following beneficial effects:

[0025] A water flow channel is formed in the incubator using a partition plate, and a flow rate control unit is used to realize water circulation in the incubator, forming an internal circulation flow mode; during the cultivation process of algae and clams, the internal circulation flow mode is adopted, and there is no need to introduce external water bodies, which can reduce the exchange and contact between the experimental water and the outside. In combination with the water environment control system, the natural habitat environment of algae and clams can be simulated more finely; the corners of the water flow channel are designed as arcs, which are more in line with the hydraulic characteristics; there is a certain distance between the two functional areas in the direction of water flow, which can improve the stability of the water environment in the second area where the attachment matrix device is located.

[0026] It can be seen that the present invention has a simple structure and is applied to the indoor artificial propagation of freshwater shellfish, which can improve the ecological balance of freshwater shellfish and algae and promote the artificial cultivation survival rate of freshwater shellfish. Practice has shown that the algae-shellfish symbiotic cultivation device of the present invention can realize the indoor cultivation and propagation of freshwater shellfish for one year, and the shellfish survival rate is above 80%. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The above and other objects, features and advantages of the present invention will become more apparent by describing in more detail exemplary embodiments of the present invention in conjunction with the accompanying drawings, wherein like reference numerals generally represent like components throughout the exemplary embodiments of the present invention.

[0028] Figure 1 is a three-dimensional diagram of an algae-shellfish symbiotic cultivation device (with the lid open) according to an embodiment of the present invention;

[0029] Figure 2 for Figure 1 A top view of

[0030] Figure 3 1 is a top view of the algae-shellfish symbiotic cultivation device (with the lid in a closed state) according to an embodiment of the present invention;

[0031] Figure 4 yes Figure 1 Schematic diagram of the water stop baffle, wherein Figure (a) is the main view and Figure (b) is the side view;

[0032] Figure 5 It is the structural diagram of the water environment control system;

[0033] Figure 6 is a schematic diagram of a lighting unit;

[0034] Figure 7 is a schematic diagram of the substrate attachment apparatus;

[0035] Figure 8 is a schematic diagram of a flow stabilizing grid plate;

[0036] Figure 9 It is a schematic diagram and a partial enlarged view of the trash grille.

[0037] In the figure: box body 11, box cover 12, water tank 13, first area 14, second area 15, water flow channel 16, partition plate 21, water stop partition 31, positioner channel 32, water inlet side 33, water outlet side 34, attachment matrix device 4, frame 41, attachment material 42, rock 43, pebble 44, concrete block 45, water environment control system 5, control unit 51, switch valve 52, illumination unit 53, bracket 531, LED light source 532, screw 533, screw buckle 534, crossbeam 535, heating unit 54, dissolved oxygen unit 55, submersible pump 61, water outlet pipe 62, flow stabilizing grid plate 71, flow stabilizing grid 72, trash grille plate 81, trash grille 82, trash intercepting trough 91, filter port 92. DETAILED DESCRIPTION

[0038] Embodiments of the present invention will now be described in greater detail with reference to the accompanying drawings. While the accompanying drawings illustrate embodiments of the present invention, it should be understood that the present invention may be implemented in various forms and should not be limited by the embodiments described herein. Rather, these embodiments are provided to make the present invention more thorough and complete, and to fully convey the scope of the present invention to those skilled in the art.

[0039] The terms used in this invention are for the purpose of describing specific embodiments only and are not intended to limit the invention. The singular forms "a," "the," and "the" used in this invention and the appended claims are also intended to include plural forms unless the context clearly indicates otherwise. It should also be understood that the term "and / or" as used herein refers to and includes any or all possible combinations of one or more of the associated listed items.

[0040] It should be understood that although the terms "first", "second", "third", etc. may be used to describe various information in the present invention, such information should not be limited to these terms. These terms are only used to distinguish information of the same type from each other. For example, without departing from the scope of the present invention, the first information may also be referred to as the second information, and similarly, the second information may also be referred to as the first information. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of such features. In the description of the present invention, "plurality" means two or more, unless otherwise clearly and specifically defined.

[0041] In the related art, indoor experiments have also been conducted because freshwater husks have high requirements for the control of the indoor growth and reproduction environment. Freshwater husks are difficult to survive for a long time indoors. The survival time is generally less than one month, the survival rate is less than 40%, and the larvae cannot survive effectively. How to make freshwater husks survive for a long time indoors to grow and reproduce, and lay the foundation for the basic ecology and environmental tolerance research of freshwater husks, is also the top priority of this invention.

[0042] To address the above problems, an embodiment of the present invention provides a single-box algae-shellfish symbiotic cultivation device, which can improve the ecological balance of freshwater shellfish and algae, promote the survival rate of artificial cultivation of freshwater shellfish, and realize indoor artificial reproduction of freshwater shellfish.

[0043] The technical solutions of the embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0044] See also Figure 1-Figure 3 An embodiment of the present invention provides a single-box algae-shellfish symbiotic cultivation device, including a culture box, a water environment control system and an attachment matrix device 4.

[0045] In some embodiments, the incubator includes a box body 11 and a box cover 12 .

[0046] A water tank 13 for holding water for the experiment is formed in the box 11. The water tank 13, as the functional part of the box 11 for holding water, can be obtained by enclosing the side panels of the box 11 on the bottom plate of the box 11. The cross-section of the water tank 13 can be runway-shaped or elliptical. In at least one embodiment, the cross-sectional shape of the water tank 13 is: two semicircles of equal radius connect the two short sides of the rectangle respectively. In this embodiment, the approximate dimensions of the water tank 13 are a total length of 140 cm, a width of 40 cm, and a height of 60 cm, wherein the length of the rectangular part is 100 cm and the radius of the semicircular part is 20 cm.

[0047] In some embodiments, the partition plate 21 is vertically installed in the housing 11. Water channels 16 are formed between the two sides of the partition plate 21 and the corresponding inner walls of the housing 11. Two functional areas are formed in the housing 11, one on each side of the partition plate 21: a first area 14 and a second area 15. Both the first area 14 and the second area 15 are located on the water channels 16. Furthermore, the partition plate 21 is arranged along the long axis of the housing 11 and is equal in length to the long side of the rectangular portion.

[0048] In this embodiment, the approximate dimensions of the partition plate 21 are 100 cm in length and 60 cm in height. The partition plate 21 is erected in the water tank 13. By forming a water flow channel 16 in the single-chamber water tank, the single-chamber water tank is transformed into a circulating water tank.

[0049] The functional area where the attachment matrix device 4 is located is the second area 15, and the functional area where the flow rate control unit is located is the first area 14. The first area 14 and the second area 15 on both sides of the partition plate 21 are two straight channels, and the length of the straight channel is approximately 100 cm, the width is 20 cm, and the height is 60 cm.

[0050] Furthermore, the flow rate control unit includes a water-stop baffle 31 and a submersible pump 61, which are used to control the circulation of water in the box 11 and regulate the water flow rate. In some embodiments, the water-stop baffle 31 is vertically installed in the box 11, specifically, in the first area 14; one end of the water-stop baffle 31 is vertically connected to the partition plate 21, and the other end is vertically connected to the inner wall of the box 11. The approximate dimensions of the water-stop baffle 31 are 20 cm in length and 60 cm in height. The water-stop baffle 31 is installed in the straight channel of the first area 14 to block the water flow.

[0051] See Figure 4Furthermore, the water-stop baffle 31 is provided with multiple retainer holes 32, each of which is a circular hole tube structure. In some embodiments, the water-stop baffle 31 is provided with a 3×3 retainer hole array, each with a 25 mm diameter and a 100 mm length. A submersible pump 61 is connected to one retainer hole 32 via an outlet pipe 62. Specifically, the outlet pipe 62 passes through the retainer hole 32 and is fixed to the water-stop baffle 31. When the submersible pump 61 is activated, water from the inlet side 33 of the water-stop baffle 31 flows out of the outlet side 34 through the outlet pipe 62, thereby circulating the water within the housing 11. Furthermore, the water flow rate can be controlled by adjusting the power of the submersible pump 61. The submersible pump 61 can be a DC submersible pump with a rated voltage of 0-72 V, a rated power of 0-500 W, a rated flow rate of 0-8 m³ / h, and a water outlet diameter of 25 mm.

[0052] by Figure 1-2 For example, when the submersible pump 61 is started, the water on the water inlet side 33 of the water-stop baffle 31 passes through the water-stop baffle 31, passes through the first area 14 and the second area 15 in sequence, and returns to the water inlet side 33 of the water-stop baffle 31, thereby forming an internal circulation flow in a clockwise direction in the water tank 13. In the process of cultivating algae and mussels, the internal circulation mode is adopted, and there is no need to introduce external water bodies, which can reduce the exchange contact between the experimental water body and the outside, and can better simulate the algae and mussel habitat environment. As a result, the water body in the water tank 13 can flow, which can be called the internal circulation mode. Different from this, the external circulation mode can be understood as the water tank 13 having an inlet end and a water outlet end, and the outlet end of the water tank 13 is connected to the water inlet end of the water tank 13 through an external water collecting tank to achieve the purpose of water circulation.

[0053] In this embodiment, there is a certain distance between the first area 14 and the second area 15 on the water flow channel 16. Therefore, after controlling the water environment, the stability of the water environment in the second area 15 where the attachment matrix device 4 is located can be improved.

[0054] The purpose of piercing a plurality of positioner channels 32 on the above water-stop baffle 31 is to adjust the height of the water outlet and the direction of water outlet. The plurality of positioner channels 32 are distributed at different heights and positions on the water-stop baffle 31. By fixing the outlet pipe 62 in different positioner channels 32, the purpose of adjusting the height of the water outlet and the direction of water outlet can be achieved. In combination with adjusting the working power of the submersible pump 61, the purpose of regulating the flow rate of the water can be achieved. It should be noted that when the submersible pump 61 is started, except for the positioner channel 32 to which the outlet pipe 62 is fixed, the other positioner channels 32 remain closed. Furthermore, the positioner channel 32 is also provided with a switch valve 52, which controls whether the positioner channel 32 is turned on or off. Among them Figure 4 The switch valve 52 is only for illustration, indicating that the switch valve is in a closed state in normal state, and the positioner channel 32 is in a blocked state.

[0055] The conduction of different positioner channels 32 can correspond to different flow rates. This is based on the different water outlet heights and water outlet directions of different positioner channels 32. Therefore, it can cooperate with the submersible pump 61 to regulate the water flow rate.

[0056] In some embodiments, the water environment control system 5 further includes one or more of an illumination unit 53, a heating unit 54, and a dissolved oxygen unit 55, and one or more of the illumination unit 53, the heating unit 54, and the dissolved oxygen unit 55 are connected to the control unit 51 and controlled by the control unit 51. The illumination unit 53, the heating unit 54, and the dissolved oxygen unit 55 are also arranged in the second area 15, but are separated from the submersible pump 61 on the opposite side of the water-stop baffle 31, wherein the illumination unit 53, the heating unit 54, and the dissolved oxygen unit 55 are located on the outlet side 34 of the water-stop baffle 31, and the submersible pump 61 is located on the inlet side 33 of the water-stop baffle 31. The control unit 51 can be an integrated controller or a combination of multiple split controllers. In this embodiment, the control unit 51 can include a flow rate controller, which is connected to the submersible pump 61.

[0057] like Figure 6 As shown, in at least one embodiment, the lighting unit 53 includes a bracket 531 and an LED light source 532 mounted on the bracket 531. The LED light source 532 is connected to and controlled by the control unit 51. The LED light source 532 is also configured to be height-adjustable. In one embodiment, the control unit 51 may include an LED lighting controller electrically connected to the LED light source 532. The LED light source 532 may be an LED tube having a length of 60 cm, a color temperature of 6500K, and a cool white light source. The LED tube is fixed to the bracket 531. 0-5 LED tubes can be mounted and adjusted on the bracket 531. The LED tubes are distributed along the direction of the water flow. The bracket 531 is suspended above the second area 15. Bracket 531 is I-shaped, 50 cm long and 15 cm wide. A 26 cm long screw 533 is located directly above the center of I-shaped bracket 531. Screw 533 is connected to the center of a crossbeam 535 above water channel 16 via a screw buckle 534. Crossbeam 535 is mounted on partition 21 and housing 11. The height of bracket 531 on crossbeam 535 can be adjusted by adjusting the upper and lower screw buckles 534, thereby adjusting the height of bracket 531 from the bottom of sink 13. The combination of screw 533, screw buckle 534, and crossbeam 535 is referred to as the height adjuster for bracket 531. The height adjuster can be adjusted from 0 to 25 cm. It works in conjunction with an LED light controller to adjust the operating hours of the LED lamps, thereby regulating the intensity and duration of the light.

[0058] In at least one embodiment, the heating unit 54 includes a heating element, which is located below the illumination unit 53 and installed at the bottom of the housing 11. The heating element is connected to the control unit 51. In one embodiment, the control unit 51 may include a temperature controller, and the heating element may be a digital display heating rod. The digital display heating rod is made of a long rectangular ceramic heating material with a rated power of 100W and a temperature control range of 18°C-35°C. The approximate dimensions are 15 cm in length, 4 cm in width, and 3 cm in height. The digital display heating rod is located below the LED light source 532, and its back suction cup is fixed to the bottom of the water tank 13. It cooperates with the temperature controller to adjust the working state to achieve regulation of the water temperature.

[0059] In at least one embodiment, the dissolved oxygen unit 55 includes an aeration stone, an oxygen tube and an oxygen pump. The aeration stone is located below the LED light source 532 and is installed at the bottom of the box 11. The oxygen pump is connected to the aeration stone through the oxygen tube, and the oxygen pump is electrically connected to the control unit 51. In one embodiment, the control unit 51 may include a dissolved oxygen controller. The aeration stone can be a high-density diamond fine sand disc with a diameter of 4cm-6cm and a thickness of 1.5cm. The rated operating voltage of the oxygen pump is AC220V±10%, the rated power is 12W, and the air output is 0-20L / min. The aeration stone is below the LED light source 532, and the back suction cup of the aeration stone is fixed to the bottom of the water tank 13. The aeration stone is connected to the oxygen pump through the oxygen tube, and cooperates with the dissolved oxygen controller to adjust the working state to achieve regulation of dissolved oxygen in the water body.

[0060] In at least one embodiment, the water environment control system 5 further includes a display unit, which is connected to the control unit 51 and is used to display flow rate information, as well as at least one of temperature information, light information, and dissolved oxygen information. The display includes a digital display control screen, which is a touch-sensitive OLED screen. The submersible pump 61, the heater, the LED lamp, and the aerator are independently controlled by the various controllers in the control unit 51 to display and monitor relevant information. For example, through the control of the control unit 51, the submersible pump 61 is set to control the water flow rate to 0.1 m / s-0.2 m / s, the digital display heater is set to control the water temperature to 22°C-24°C, and the aerator is set to control the dissolved oxygen in the water to 6 mg / L-9 mg / L.

[0061] like Figure 7 As shown, further, the attachment matrix device 4 includes a frame 41 and at least one type of attachment material 42, the attachment material 42 is laid in the frame 41, and the at least one type of attachment material 42 includes at least one type of rock 43, pebbles 44, and concrete blocks 45. The attachment matrix device 4 includes at least two types of attachment materials 42, and different types of attachment materials 42 are laid in different positions in the frame 41.

[0062] Among them, the second area 15 is the experimental area, the frame 41 is rectangular, with an approximate size of 80 cm in length and 20 cm in width, and is arranged in the center of the second area 15. The frame 41 is embedded in the bottom of the second area 15, and the matrix material for inducing the attachment of freshwater shellfish is evenly spread in the frame 41. In this embodiment, the attachment materials 42 include three types: rocks 43, pebbles 44 and concrete blocks 45. The paving area is combined in a 1:1:1 ratio. The particle size range of each is 5 cm-12 cm, and the porosity is controlled to be 10%-20%. When the freshwater shellfish and algae in the water tank 13 are cultivated, the box cover 12 at least fully covers the second area 15. The box cover 12 is an openable and closable light-shielding and heat-insulating cover, such as a black opaque foam KT board. The approximate size of the box cover 12 is 95 cm in length and 40 cm in width. Obviously, the box cover 12 can also cover the first area 14.

[0063] As a preferred embodiment of the present invention, it also includes a flow stabilizing grid plate 71 and a trash blocking grid plate 81, which are respectively arranged at the two ends of the partition plate 21, and the two ends of the partition plate 21 are respectively recorded as the first end and the second end, wherein the flow stabilizing grid plate 71 is connected to the second end of the partition plate 21, and the trash blocking grid plate 81 is connected to the first end of the partition plate 21; and the flow stabilizing grid plate 71 is located in the second area 15, and the trash blocking grid plate 81 is located in the first area 14.

[0064] like Figure 8 As shown, in at least one embodiment, one end of the flow stabilizing grid plate 71 is vertically connected to the second end of the partition plate 21, and the other end is connected to the inner wall of the box body 11. The flow stabilizing grid plate 71 and the substrate attachment device 4 are on the same side of the partition plate 21. The flow stabilizing grid plate 71 has an approximate size of 60 cm in height and 40 cm in width. An array of rectangular flow stabilizing grids 72 is provided on the flow stabilizing grid plate 71. The rectangular flow stabilizing grids 72 are 1 cm × 3 cm in size and are longitudinally arranged along their long sides. The spacing between adjacent rectangular flow stabilizing grids 72 on the left and right is 1 cm, and the spacing between adjacent rectangular flow stabilizing grids 72 on the top and bottom is 1 cm. The two sides of the flow stabilizing grid plate 71 are vertically fixed to the partition plate 21 and the inner wall of the box body 11 through slots, respectively, to stabilize the water flow rate.

[0065] like Figure 9 As shown, in at least one embodiment, one end of the trash grille 81 is perpendicularly connected to the first end of the partition plate 21, and the other end is connected to the inner wall of the housing 11. The trash grille 81 and the water-stop baffle 31 are located on the same side of the partition plate 21. In this embodiment, the water-stop baffle 31 is located near the first end of the partition plate 21, and a chamber for accommodating the submersible pump 61 is formed between the water-stop baffle 31 and the trash grille 81. While ensuring normal water permeability, the trash grille 81 is used to intercept large floating and suspended debris in the water to prevent it from affecting the normal operation of the submersible pump 61.

[0066] The trash screen 81 is equipped with an array of rectangular trash screens 82, which are smaller than the rectangular flow-stabilizing screens 72. The approximate dimensions of the trash screen 81 are 60 cm in height and 40 cm in width. The rectangular trash screens 82 measure 0.8 cm x 2 cm, with their long sides arranged horizontally. Trash screen 81 is secured vertically to the partition plate 21 and the inner wall of the housing 11 via slots at both ends. Trash screen 81 is designed to intercept larger floating and suspended matter in the water while ensuring normal water permeability, preventing it from affecting the normal operation of the submersible pump.

[0067] As a preferred embodiment of the present invention, a sewage interception trough 91 is further provided at the bottom of the box body 11. In at least one embodiment, the sewage interception trough 91 is connected to the first end of the partition plate 21 at the bottom of the water flow channel 16 and is located on the extension line of the partition plate 21. In this embodiment, a filter port 92 is provided at the bottom of the sewage interception trough 91, and the cross-section of the sewage interception trough 91 is a trapezoid with the upper base longer than the lower base. The sewage interception trough 91 is provided upstream of the sewage grid plate 81 and downstream of the attachment matrix device 4. The sewage interception trough 91 is located at the bottom of the water tank 13, and a filter port 92 is provided on the lower bottom of the sewage interception trough 91. The filter port 92 is generally in a closed state and is opened when needed to discharge larger suspended and precipitated substances in the water body. The sewage interception trough 91 is on the extension line of the partition plate 21. The approximate dimensions of the sewage interception trough 91 are a length of 20 cm, a trapezoidal cross-section, an upper bottom width of 2 cm, a lower bottom width of 1.5 cm, and a depth of 1 cm. A filter port 92 is set at the center of the bottom of the sewage interception trough 91. The filter port 92 is a circular hole with a pore diameter of 1.5 cm. The filter port 92 can be connected to an external drain pipe to intercept and discharge larger suspended and precipitated substances brought about during the flow of water.

[0068] It can be seen that the single-box algae and shellfish symbiotic culture device provided by the embodiment of the present invention has a simple structure and adopts a semicircular design at the corner of the water flow channel 16, which is more in line with hydraulic characteristics. At the same time, the internal circulation mode can reduce the experimental water body from exogenous exchange contact, and the water environment control system 5 that can regulate the water environment can be used to simulate the natural habitat environment of algae and shellfish more finely, significantly improve the survival rate of indoor freshwater shellfish artificial cultivation, and ensure long-term stability and effectiveness. Practice has proved that the algae and shellfish symbiotic culture device of the present invention can realize the indoor cultivation and breeding of freshwater shellfish for one year, and the shellfish survival rate is more than 80%.

[0069] More specifically, after the present invention is used to cultivate freshwater shellfish for a period of time, the growth and reproduction of larvae can be observed. Therefore, the present invention can realize the larval reproduction and adult cultivation of freshwater shellfish in the water tank 13, and realize the artificial reproduction of freshwater shellfish indoors.

[0070] The embodiment of the present invention also provides an artificial cultivation method using the above indoor algae-shellfish symbiotic artificial cultivation system, comprising:

[0071] S100: Pour water into the water tank 13 of the box 11;

[0072] In step S100 , water for the experiment is added to the water tank 13 , and the water depth is controlled to be (20±1) cm.

[0073] S200: adding shellfish to the attachment matrix device 4;

[0074] In step S200, live freshwater shellfish collected from the wild are added to the attachment matrix device 4, wherein the culture density is controlled to be 900 / m 2 -1100 pieces / m 2 Shellfish stocking density refers to the number of shellfish per unit area of the substrate attachment device.

[0075] S300: adding algae to the second area;

[0076] In step S300, artificially cultured green algae liquid is added to the outlet side of the water-stop baffle 31 and below the LED lamp, and the algae cultivation density in the water tank 13 is controlled to be 3×10 5 -5×10 5 cell / L. Algae cultivation density refers to the number of algae cells per unit water body.

[0077] S400: Regulating water flow rate, light intensity, water temperature, and water dissolved oxygen using the flow rate control unit, light unit, heating unit 54, and dissolved oxygen unit 55;

[0078] In step S400, the flow rate control unit is used to control the water to circulate at a flow rate of 0.1-0.2 m / s; the light intensity is controlled to 8500 Lux-8700 Lux, and the working and intermittent time of the LED lamp is set to 12h:12h; the water temperature is controlled to (23±1)°C using the heating unit 54; and the dissolved oxygen unit 55 is used to control the dissolved oxygen in the water to 6-9 mg / L.

[0079] S500: After the above process is completed, the box cover 12 is closed to achieve the effect of heat preservation and light shielding, so as to keep the freshwater shellfish in a dark environment.

[0080] After the freshwater shellfish have grown stably for a certain period of time, the lid 12 can be opened to observe the growth of the freshwater shellfish in the water tank 13, or to take out some living freshwater shellfish for research and experiment.

[0081] In particular, the algae-shellfish symbiotic culture device of the present invention can achieve the growth of freshwater shellfish within one year, during which time the freshwater shellfish reproduces larvae and cultivates the shellfish. Therefore, the optimal hydrological rhythm required for the reproduction of freshwater shellfish can be determined first, and the optimal hydrological rhythm for the reproduction of freshwater shellfish can be simulated by regulating the water temperature and flow rate in the water tank, and the algae food in the water tank is controlled to be sufficient. After the freshwater shellfish has been stably attached and grown for a certain period of time, normal reproduction of larvae and cultivation of shellfish can be observed.

[0082] While various embodiments of the present invention have been described above, the above descriptions are intended to be illustrative, non-exhaustive, and not limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is selected to best explain the principles of the embodiments, their practical applications, or improvements to existing technologies, or to enable others skilled in the art to understand the embodiments disclosed herein.

Claims

1. An indoor algae-shellfish symbiotic artificial cultivation system, characterized by: The invention comprises a culture box with a box cover, an attachment substrate device and a water environment control system; A partition plate is vertically provided in the incubator to form a water flow channel between the partition plate and the inner wall of the incubator, and two functional areas are formed on both sides of the partition plate: a first area and a second area; The attachment matrix device is arranged in the second area; The water environment control system includes a flow rate control unit, a lighting unit, a heating unit, a dissolved oxygen unit and a control unit, wherein the flow rate control unit, the lighting unit, the heating unit and the dissolved oxygen unit are all arranged in the first area and are all controlled by the control unit; The flow rate control unit includes: a water-stop baffle erected in the first area, one end of which is connected to the partition plate and the other end is connected to the inner wall of the incubator; a submersible pump located on the water inlet side of the water-stop baffle, the submersible pump being connected to a hole provided on the water-stop baffle via a water outlet pipe or the water outlet pipe passing through the hole; when the submersible pump is started, water on the water inlet side of the water-stop baffle flows out from the water outlet side of the water-stop baffle via the water outlet pipe, causing the water contained in the incubator to circulate along the water flow channel; Among them, the illumination unit, the heating unit and the dissolved oxygen unit are all arranged on the water outlet side of the water-stop baffle.

2. The indoor algae-shellfish symbiotic artificial cultivation system according to claim 1, wherein: The cross section of the incubator is in a racetrack shape or an ellipse shape.

3. The indoor algae-shellfish symbiotic artificial cultivation system according to claim 1, wherein: The partition plate is arranged along the long axis direction of the cross section of the incubator.

4. The indoor algae-shellfish symbiotic artificial cultivation system according to claim 1, wherein: A plurality of channels are provided on the water-stop baffle, and the channels are positioner channels. The positioner channels are provided with a switch valve, which controls the conduction or isolation of the positioner channels. The water outlet pipe passes through one of the positioner channels.

5. The indoor algae-shellfish symbiotic artificial cultivation system according to claim 1, wherein: The lighting unit includes a light source suspended above the second area.

6. The indoor algae-shellfish symbiotic artificial cultivation system according to claim 1, wherein: The attachment matrix device comprises a frame and at least one type of attachment material: the frame is arranged at the bottom of the second area; and the attachment material is laid in the frame.

7. The indoor algae-shellfish symbiotic artificial cultivation system according to claim 1, wherein: A flow stabilizing grid plate and a trash grille plate are vertically arranged in the incubator, and the flow stabilizing grid plate and the trash grille plate are respectively arranged at the two ends of the partition plate; wherein, the trash grille plate and the water-stop partition plate are located on the same side of the partition plate, one end of the trash grille plate is connected to the first end of the partition plate, and the other end thereof is connected to the inner wall of the incubator; the submersible pump is located between the trash grille plate and the water-stop partition plate; the flow stabilizing grid plate and the attachment matrix device are located on the same side of the partition plate, one end of the flow stabilizing grid plate is connected to the second end of the partition plate, and the other end thereof is connected to the inner wall of the incubator.

8. The indoor algae-shellfish symbiotic artificial cultivation system according to claim 1, wherein: The bottom of the incubator is provided with a sewage interception trough, which is arranged on the extension line of the partition plate and close to the water inlet side of the water-stop partition plate; a filter port is provided at the bottom of the sewage interception trough.

9. A method for artificially cultivating algae and shellfish symbiosis indoors, characterized by: Artificial cultivation is performed using the indoor algae-shellfish symbiotic artificial cultivation system according to any one of claims 1 to 8, comprising: Fill the incubator with water; Adding shellfish to the attachment substrate device; adding algae to the second area on the outlet side of the water stop baffle; Use the flow rate control unit, lighting unit, heating unit, and dissolved oxygen unit to control the water flow rate, light intensity, water temperature, and dissolved oxygen in the water to reach the preset values; Close the lid of the box.

10. The indoor algae-shellfish symbiotic artificial cultivation method according to claim 9, characterized in that: The stocking density of the shellfish is 900 / m 2 -1100 pieces / m 2 ; and the algae cultivation density in the incubator is 3×10 5 -5×10 5 cell / L.

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