Application of chidaaniline in promoting growth of panax notoginseng and preventing and treating diseases

Abstract

The invention relates to the technical field of plant disease prevention and control, in particular to application of chidaaniline in promoting growth of panax notoginseng and preventing and controlling diseases. In one aspect, the invention provides the application of the chidaaniline in preventing and treating panax notoginseng root rot, and the chidaaniline increases the growth of beneficial bacteria trichoderma and mortierella. In another aspect, the invention provides the application of the chidaaniline in promoting the growth of the pseudo-ginseng, and the chidaaniline increases the seedling survival rate of the pseudo-ginseng, increases the dry weight of a single plant on the ground of the pseudo-ginseng and increases the dry weight of a single plant under the ground of the pseudo-ginseng.

Description

Technical Field

[0001] The present invention relates to the technical field of plant disease prevention and control, and in particular to application of cedaranid in promoting growth of Panax notoginseng and preventing and controlling diseases. Background Art

[0002] Chidamide (1616493-44-7), molecular formula: C 22 H 19 FN4O2, molecular weight: 390.41, comes from pine root secretions.

[0003] Obstacles to continuous cropping of Panax notoginseng are gradually emerging in Yunnan, a key production area, manifesting as soil degradation, decreased seed germination rate, poor plant growth and frequent root rot. In severe cases, this may lead to a significant drop in yield or even no harvest. In order to improve soil conditions, long-term crop rotation is necessary, but its implementation is quite challenging. Although chemical pesticides can control pests and diseases in the short term, they are unstable and may pollute the environment. Although effective, techniques such as soil fumigation may damage the quality of medicinal materials and soil health. Faced with the need to improve the quality of Panax notoginseng and the complexity of root rot, existing prevention and control measures are insufficient, and there is an urgent need to develop new technologies to promote the growth of Panax notoginseng and effectively prevent and control root rot. Summary of the Invention

[0004] The present invention provides the use of cedaniline for promoting the growth of Panax notoginseng and preventing and controlling diseases, thereby addressing the shortcomings of the prior art in lacking methods for promoting the growth of Panax notoginseng and preventing and controlling root rot. In particular, the present invention provides the use of cedaniline or its derivatives or pharmaceutically acceptable salts for promoting the growth of beneficial microorganisms of the genera Mortierella and Trichoderma in the rhizosphere of Panax notoginseng, thereby indirectly preventing and controlling root rot of Panax notoginseng caused by Fusarium.

[0005] The present invention provides a plant beneficial bacteria growth promoter, characterized in that the plant beneficial bacteria growth promoter comprises cedaniline or its derivatives or pharmaceutically acceptable salts thereof.

[0006] Preferably, the plant beneficial bacteria are Trichoderma and / or Mortierella.

[0007] The present invention provides application of cedaniline or its derivatives or pharmaceutically acceptable salts in preventing and treating Panax notoginseng root rot.

[0008] The present invention provides the use of cedaniline or its derivatives or pharmaceutically acceptable salts in promoting the growth of Panax notoginseng.

[0009] Preferably, the cedaniline or its derivative or pharmaceutically acceptable salt thereof increases the dry weight or fresh weight of Panax notoginseng.

[0010] Preferably, the sidanilide or its derivative or pharmaceutically acceptable salt thereof increases the dry weight or fresh weight of the roots and / or leaves of Panax notoginseng.

[0011] Preferably, the sidanilide or its derivatives or pharmaceutically acceptable salts thereof increase the survival rate of Panax notoginseng seedlings, increase the dry weight of Panax notoginseng plants above ground, and increase the dry weight of Panax notoginseng plants underground.

[0012] Preferably, the concentration of cedaniline or its derivative or pharmaceutically acceptable salt is not less than 0.0001 ppm.

[0013] Preferably, the concentration of cedaniline or its derivative or pharmaceutically acceptable salt thereof is 0.0001 ppm-0.1 ppm.

[0014] The present invention provides the use of cedaniline or its derivatives or pharmaceutically acceptable salts thereof in improving the survival rate of Panax notoginseng seedlings at a concentration of 0.01 ppm.

[0015] The present invention provides the use of cedaniline or its derivatives or pharmaceutically acceptable salts thereof in increasing the dry weight of a single Panax notoginseng plant at a concentration of 0.01 ppm.

[0016] The present invention provides the use of cedaniline or its derivatives or pharmaceutically acceptable salts thereof in increasing the dry weight of individual underground Panax notoginseng plants at a concentration of 0.01 ppm.

[0017] When the concentration of cedaraniline is greater than 0.0001ppm, the growth of the beneficial bacteria Trichoderma can be increased. The experimental concentration range is 0.0001-0.1ppm, which can increase the growth of Trichoderma. The growth-promoting effect is best at 0.1ppm.

[0018] Beneficial effects of the present invention:

[0019] 1) The sidanilide or its derivatives or pharmaceutically acceptable salts described in the present invention are of great significance in preventing and treating root rot caused by Fusarium oxysporum and rust fungi, especially indirectly promoting the growth of beneficial fungi Mortierella and Trichoderma, thereby effectively preventing and treating Panax notoginseng root rot, and providing a new control agent for preventing and controlling Panax notoginseng diseases.

[0020] 2) Sidanilide can increase the survival rate of Panax notoginseng seedlings and the dry weight of the aboveground and underground parts of individual plants, which meets the requirements of sustainable development and has broad prospects for research and market application.

[0021] 3) The present invention provides an application of cedaniline, a metabolite secreted from pine tree roots, in Panax notoginseng, which can not only prevent and treat Panax notoginseng root rot but also increase the dry weight of Panax notoginseng. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions in the present invention or the prior art, a brief introduction is given below to the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0023] Figure 1 This is the growth-promoting effect of cedaniline on Trichoderma provided in Example 1 of the present invention.

[0024] Figure 2 This is a photo of the drug-treated plate of Trichoderma using cedaniline provided in Example 1 of the present invention.

[0025] Figure 3 This is the growth-promoting effect of cedaniline on Mortierella provided in Example 2 of the present invention.

[0026] Figure 4 This is a photo of the drug-treated plate of cedaniline against Mortierella provided in Example 2 of the present invention.

[0027] Figure 5 This is the effect of sidanilide on the survival rate of Panax notoginseng provided in Example 3 of the present invention.

[0028] Figure 6 This is the effect of cedaniline on the aboveground dry weight of Panax notoginseng individual plants provided in Example 4 of the present invention.

[0029] Figure 7 This is a diagram showing the effect of cedaniline on the underground dry weight of Panax notoginseng potted plants provided in Example 4 of the present invention.

[0030] Figure 8 This is a drug-containing plate for Mortierella sp. using 3-methoxytyramine provided in Comparative Example 1 of the present invention.

[0031] Figure 9 This is a diagram showing the inhibitory effect of 3-methoxytyramine on Mortierella provided in Comparative Example 1 of the present invention.

[0032] Figure 10 This is the effect of palmitic acid on the survival rate of Panax notoginseng provided in Comparative Example 2 of the present invention.

[0033] Figure 11 This is the effect of palmitic acid provided in Comparative Example 2 of the present invention on the dry weight of individual Panax notoginseng plants above ground.

[0034] Figure 12 This is the effect of palmitic acid provided in Comparative Example 2 of the present invention on the dry weight of individual underground Panax notoginseng plants.

[0035] In the figure, CK is the control group. DETAILED DESCRIPTION

[0036] To make the objectives, technical solutions, and advantages of the present invention more clear, the technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts shall fall within the scope of protection of the present invention.

[0037] Unless otherwise specified, the reagents and materials used in the following examples were commercially available.

[0038] Example 1

[0039] This example verifies the growth-promoting effect of cedaraniline on Trichoderma through a bottle culture experiment. The specific experimental steps are as follows:

[0040] 1. Prepare the compound into a solution: Dissolve 10 mg of cedaraniline in 10 ml of methanol to prepare a mother solution.

[0041] Take 100μL of ① and add 900μL of methanol →②

[0042] Take 100μL of ② and add 900μL of methanol → ③

[0043] Take 100μL of ③ and add 900μL of methanol → ④

[0044] The control is methanol →⑤

[0045] 2. After sterilization, pipette 400 μL of each of ④-① into 400 mL of culture medium to obtain culture medium with concentrations of 0.0001 ppm, 0.001 ppm, 0.01 ppm, and 0.1 ppm, respectively. For the control group, add 1 mL of methanol solution to 99 mL of culture medium. Shake well, pour onto a plate, and cool. Use a 5 mm diameter punch to punch the rust fungus into approximately 5 mm cakes. Use an inoculating needle to inoculate this cake into the culture medium containing the compound solution. Place in an incubator at 26-28°C for 2 days. After 2 days, observe the colony morphology and measure the colony diameter.

[0046] like Figure 1 、 Figure 2 As shown, the results showed that when the concentration of cedaraniline was 0.0001ppm, the colony diameter of Trichoderma was significantly higher than that of the control group, indicating that cedaraniline had a significant growth-promoting effect on Trichoderma; when the concentration of cedaraniline continued to increase, the promotion effect did not increase significantly, so the usage range of cedaraniline was 0.0001ppm-0.1ppm, and the effect was best at low concentrations.

[0047] Example 2

[0048] This example verifies the growth-promoting effect of cedaraniline on Mortierella through a bottle culture experiment. The specific experimental steps are as follows:

[0049] 1. Prepare the compound into a solution: Dissolve 10 mg of cedaraniline in 10 ml of methanol to prepare a mother solution.

[0050] Take 100μL of ① and add 900μL of methanol →②

[0051] Take 100μL of ② and add 900μL of methanol → ③

[0052] Take 100μL of ③ and add 900μL of methanol → ④

[0053] The control is methanol →⑤

[0054] 2. After sterilization, pipette 400 μL of each of ④-① into 400 mL of culture medium to obtain culture medium with concentrations of 0.0001 ppm, 0.001 ppm, 0.01 ppm, and 0.1 ppm, respectively. For the control group, add 1 mL of methanol solution to 99 mL of culture medium. Shake well, pour onto a plate, and cool. Use a 5 mm diameter punch to punch the fungus into approximately 5 mm cakes. Use an inoculating needle to inoculate this cake into the culture medium containing the compound solution. Place in an incubator at 26-28°C for 7 days. After 7 days, observe the colony morphology and measure the colony diameter.

[0055] like Figure 3 、 Figure 4 As shown, the results showed that when the concentration of cedaraniline was 0.001ppm, the colony diameter of Mortierella was significantly higher than that of the control group, indicating that cedaraniline had a significant growth-promoting effect on Mortierella; and with the increase of concentration, the growth-promoting effect gradually increased, so the usage range of cedaraniline was 0.001ppm-0.1ppm, and the effect was best at low concentrations.

[0056] Example 3

[0057] This example verifies the effect of cedaranid on increasing the survival rate and dry weight of single-plant Panax notoginseng. The specific experimental steps are as follows:

[0058] 1. Prepare the compound into a solution: Based on the plate effect, the concentration of 0.01 ppm of cedaraniline was selected for the Panax notoginseng potted plant experiment. 0.1 mg of cedaraniline was added to 1 L of sterile water to prepare a stock solution.

[0059] 2. Take 10 ml of the mother liquor and add it to 1 L of water at a concentration of 0.01 ppm. After mixing, add 50 ml of the compound to each pot according to the concentration. For the control, take 1 ml of methanol and add it to 1 L of water. Then the root irrigation experiment continued for 6 weeks (once a week). After the Panax notoginseng continued to grow for two months, the potted Panax notoginseng was sampled, cleaned, and the number of Panax notoginseng plants treated with the gradient concentration of each compound was counted. The above-ground and underground parts were respectively placed in envelopes, fixed at 115°C for 30 minutes, and dried at 60°C for 3 days. The dry weight was weighed and calculated.

[0060] Figure 5 The results showed that root irrigation with 0.01ppm of cedaranid could significantly increase the survival rate of Panax notoginseng seedlings. Figure 6 and Figure 7 The results showed that root irrigation of cedaraniline at a concentration of 0.01ppm can significantly increase the dry weight of both aboveground and underground parts of the plant. The overall results showed that 0.01ppm can significantly improve the growth of Panax notoginseng plants.

[0061] Comparative Example 1

[0062] This comparative example provides another substance detected in root exudates - 3-Methoxytyamine (554-52-9), with the molecular formula: C9H 13 NO2, molecular weight: 167.20502. , and tested its inhibitory effect on Mortierella. The specific experimental steps differed from those in Example 1 only in that cedaniline was replaced with 3-methoxytyramine.

[0063] The results are as follows Figure 8 and Figure 9 As shown, after adding 3-methoxytyramine, at concentrations above 0.01 ppm, the diameter of the bacterial cake on the plate was significantly lower than that of the control. The results showed that 3-methoxytyramine inhibited the growth of Mortierella at concentrations of 0.01 ppm and above.

[0064] Comparative Example 2

[0065] This comparative example verified the effect of another substance detected in root exudates, palmitic acid (CAS: 10025-99-7), on the growth of Panax notoginseng potted plants through a bottle culture experiment.

[0066] The results are as follows Figure 10 、 Figure 11 and Figure 12 As shown in the results, palmitic acid could not increase the aboveground dry weight and fibrous root growth of Panax notoginseng.

[0067] In addition to the compounds listed in the above comparative examples, the present invention also verified other compounds obtained from pine root secretions. It was found that many other compounds had no obvious promoting effect on beneficial bacteria and microorganisms, which will not be listed here one by one.

[0068] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A plant beneficial bacteria growth promoter, characterized in that The plant beneficial bacteria growth promoter comprises cedaniline or its derivatives or pharmaceutically acceptable salts thereof.

2. The plant beneficial bacteria growth promoter according to claim 1, wherein The plant beneficial bacteria are Trichoderma and / or Mortierella.

3. Use of cedaraniline or its derivatives or pharmaceutically acceptable salts in preventing and treating Panax notoginseng root rot.

4. The use according to claim 3, characterized in that The cedaniline or its derivatives or pharmaceutically acceptable salts thereof increase the growth of the beneficial bacteria Trichoderma and / or Mortierella.

5. Use of cedaniline or its derivatives or pharmaceutically acceptable salts in promoting the growth of Panax notoginseng.

6. The use according to claim 5, characterized in that The sidanilide or its derivative or pharmaceutically acceptable salt thereof increases the dry weight or fresh weight of Panax notoginseng.

7. The use according to claim 6, characterized in that The sidanilide or its derivative or pharmaceutically acceptable salt thereof increases the dry weight or fresh weight of the root and / or leaf of Panax notoginseng.

8. The use according to claim 5, characterized in that The sidanilide or its derivatives or pharmaceutically acceptable salts thereof increase the survival rate of Panax notoginseng seedlings, increase the dry weight of individual Panax notoginseng plants above ground, and increase the dry weight of individual underground plants.

9. The use according to any one of claims 3 to 8, characterized in that The concentration of the cedaraniline or its derivative or its pharmaceutically acceptable salt is not less than 0.0001 ppm.

10. The use according to claim 9, characterized in that: The use concentration of the cedaniline or its derivative or pharmaceutically acceptable salt is 0.0001 ppm-0.1 ppm.

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