Instantaneous anti-wrinkle firming composition as well as preparation method and formula application thereof

Abstract

The invention belongs to the technical field of cosmetic production, and discloses an instant anti-wrinkle firming composition and a preparation method and formula application thereof, and the instant anti-wrinkle firming composition is prepared from the following components in parts by mass: 0.00005 to 0.0125 part of small molecule peptide, 0.00001 to 1 part of snake venom peptide, 0.000001 to 0.1 part of soluble collagen, 0.001 to 10.5 part of glassine and 0.001 to 7 parts of ectoine. Wherein the small molecule peptide is selected from at least one of acetyl hexapeptide-8, acetyl octapeptide-3, acetyl heptapeptide-4, palmitoyl tripeptide-5 and acetyl tetrapeptide-2, through the design, the elastase inhibition capacity of the composition is improved, and it is found through experiments that the elastase inhibition capacity of the composition is greatly improved. The composition can increase the elastase inhibition rate to a higher level in a short time.

Description

Technical Field

[0001] The present application relates to the technical field of cosmetic production, and in particular to an instant anti-wrinkle and firming composition, a preparation method thereof, and a formula application. Background Art

[0002] Skin sagging is a common aging phenomenon for most people, typically manifesting as a loss of skin tone and elasticity, along with the appearance of wrinkles and fine lines. This phenomenon not only affects a person's appearance but can also significantly impact their mental health. Understanding the dangers of skin sagging and the importance of firming and anti-wrinkle care is crucial for maintaining healthy skin and a good quality of life. First, the dangers of skin sagging manifest in several key ways. With aging, the production of collagen and elastic fibers in the skin gradually decreases, and internal and external environmental factors can accelerate the aging process. Ultraviolet radiation, oxidative stress, unhealthy lifestyle habits (such as smoking and alcohol abuse), and lack of proper skin care can all lead to a loss of elasticity and firmness. This skin sagging often results in noticeable changes in appearance, including wrinkles, blurred facial features, and sagging, making a person appear older than their actual age. Furthermore, sagging skin can also impact mental health. Many people may experience depression, low self-esteem, and even anxiety when faced with the signs of aging. Appearance is considered an integral part of many people's self-worth, and seeing their skin gradually lose its firmness and radiance can often lead to anxiety and dissatisfaction, leading to negative emotions. This psychological pressure may affect interpersonal relationships and social activities, and even affect career development. Therefore, paying attention to skin care is not only a pursuit of external beauty, but also a concern for one's own mental health. In addition, sagging skin may also lead to some functional effects. Healthy skin is not only a manifestation of beauty, but also an important barrier for the body's protection. When the skin is sagging, its protective function may be weakened, which means that the skin's ability to resist external stimuli, bacterial invasion and environmental pollution is reduced. This situation may make the skin more susceptible to injury, sensitivity, and dryness, thereby affecting overall skin health. Therefore, it is very necessary to take measures to improve the sagging of the skin.

[0003] Firming and anti-wrinkle care is particularly important for addressing sagging skin. Firstly, from a cosmetic perspective, firmer skin can significantly improve facial contours and overall complexion. Improved skin condition and changes in appearance often lead to greater self-satisfaction. Good skin fosters a positive self-image, allowing individuals to be more comfortable and natural in social situations. Self-confidence and mental health are closely linked, so good skin can alleviate the psychological stress of aging, thereby promoting physical and mental well-being. Furthermore, firming and anti-wrinkle care can help slow the aging process.

[0004] Chinese patent application 201710809821.3 discloses a skin care oil gel containing polypeptides, which is composed of the following components in parts by mass: polypeptide 0.001%-2%, emulsifier 0.5%-10%, co-emulsifier 0.1%-5%, vegetable oil 25%-85%, phytosterol ester 1%-5%, water 0.5%-3%, stearic acid 0.20%-0.75%, and glycerol as the balance. The emulsifier is a 1:1 mixture of hydrogenated lecithin and cholesterol macadamia oleate, the co-emulsifier is polyglycerol-10 myristate, and the vegetable oil is one of jojoba seed oil and macadamia nut seed oil, or a mixture thereof in any ratio.

[0005] The polypeptide-containing skin care oil gel obtained by the scheme has good stability of the polypeptide in the dispersed system, good permeability of the polypeptide on the skin, and high bioavailability. Further observation of the scheme shows that the polypeptides used in the scheme include various types, such as palmitoyl tripeptide 5, palmitoyl tetrapeptide-7, tripeptide-1 copper, nonapeptide-1, hexapeptide-1, hexapeptide-11, hexapeptide-2, hexapeptide-3, hexapeptide-5, hexapeptide-9, heptapeptide-6, myristoyl hexapeptide-5, myristoyl pentapeptide-4, trifluoroacetyl tripeptide-2, tripeptide-1, tripeptide-10 citrulline, tripeptide 2, tripeptide-32, tridecapeptide-1, decapeptide-4, tetrapeptide-1, tetrapeptide-3, tetrapeptide-4, pentapeptide-1, pentapeptide-3, pentapeptide-34 trifluoroacetate, acetyl octapeptide-3, acetyl hexapeptide-1, acetyl pentapeptide-4, trifluoroacetate, Acyl hexapeptide-7, acetyl hexapeptide-8, acetyl heptapeptide-4, acetyl tripeptide-1, acetyl tetrapeptide-11, acetyl tetrapeptide-2, acetyl tetrapeptide-3, acetyl tetrapeptide-5, acetyl tetrapeptide-9, palmitoyl dipeptide-7, palmitoyl hexapeptide-12, palmitoyl hexapeptide-14, palmitoyl hexapeptide-15, palmitoyl tripeptide-1, carnosine, palmitoyl tripeptide-8, palmitoyl tetrapeptide-10, palmitoyl tetrapeptide-5, palmitoyl pentapeptide-4, palmitoyl pentapeptide-5, oligopeptide-1, oligopeptide-2, oligopeptide 29, oligopeptide-3, oligopeptide-3, oligopeptide-32, oligopeptide-4, oligopeptide-5, oligopeptide-6, etc. However, different peptides often show different effects in actual application, and this scheme and the instructions are more about showing the gel strength performance of its product;

[0006] Chinese patent application 201910216837.2 discloses a water-soluble solution containing peptides and a preparation method thereof, wherein the aqueous solution contains hydrogenated castor oil and / or PEG-modified hydrogenated castor oil, and the peptides include palmitoyl tripeptide-1 / palmitoyl oligopeptide, palmitoyl tripeptide-5 / collagen peptide, copper peptide / tripeptide-1 copper / blue copper peptide, palmitoyl tetrapeptide-7 / palmitoyl tetrapeptide-3, acetyl tetrapeptide-9, acetyl tetrapeptide-5, palmitoyl pentapeptide-4 / M-yl peptide, pentapeptide-18, myristoyl pentapeptide-17, palmitoyl hexapeptide / palmitoyl hexapeptide-12, acetyl hexapeptide-8, acetyl octapeptide / acetylglutamyl heptapeptide-3, hexapeptide-2, hexapeptide-9, hexapeptide-10, acetyl hexapeptide-38, nonapeptide-1, and a mixture of one or more of decapeptide-12.

[0007] The water-soluble solution of this solution remains clear and transparent at high concentrations, without precipitation of impurities. The prepared peptide solution is stable, mild and non-irritating, and the preparation method is green, controllable, and low-cost. A further observation of the instructions for this solution shows that the test results in paragraph 77 of the instructions record the skin indicators of volunteers after use. Compared with the control group, the test group showed an effect of improving skin moisture, gloss, and elasticity. However, the test results in paragraph 77 show that the test time of the test group was twice a day. After 3 months of continuous application, it took slightly longer to produce a firming effect.

[0008] The problem to be solved by this solution is: how to provide a composition with anti-wrinkle and firming capabilities, and the composition can exert its anti-wrinkle and firming capabilities in a relatively short period of time. Summary of the Invention

[0009] The purpose of the present application is to provide a composition with anti-wrinkle and firming capabilities. The composition not only has good firming and anti-wrinkle capabilities through the interaction between small molecule peptides, snake venom peptides, soluble collagen, bosine and ectoine, but also enables the composition to exert its firming and anti-wrinkle capabilities in a relatively short period of time.

[0010] To achieve the above objectives, the present application discloses an instant anti-wrinkle and firming composition, which comprises the following components in parts by mass:

[0011]

[0012] The small molecule peptide is selected from at least one of acetyl hexapeptide-8, acetyl octapeptide-3, acetyl heptapeptide-4, palmitoyl tripeptide-5, and acetyl tetrapeptide-2.

[0013] It should be noted that the present application promotes cell regeneration and repair through small molecule peptides, thereby helping to reduce skin damage and improve the overall health of the skin; in addition, small molecule peptides stimulate the synthesis of collagen and elastin in the skin, enhance the firmness and elasticity of the skin, and reduce the formation of wrinkles. In addition, small molecule peptides can help maintain the moisture balance of the skin and prevent water loss by enhancing the skin barrier function, thereby achieving a good moisturizing effect.

[0014] Snake venom peptides relax muscles, reduce the formation of fine lines and wrinkles, and make the skin look smoother; in addition, snake venom peptides can stimulate the synthesis of collagen in the skin, thereby improving the elasticity and firmness of the skin;

[0015] Effectively absorbs moisture through soluble collagen, helping to maintain the skin's moisture balance, thereby providing long-lasting moisturizing effects and making the skin softer and smoother;

[0016] Bosin has good anti-irritation and soothing effects, can relieve skin redness and discomfort, and is suitable for sensitive skin;

[0017] Ectoin is a natural osmotic agent that effectively attracts water, helping to maintain the skin's moisture balance and provide long-lasting moisturization.

[0018] Preferably, the small molecule peptide is a mixture of acetyl hexapeptide-8, acetyl octapeptide-3, acetyl heptapeptide-4, palmitoyl tripeptide-5, and acetyl tetrapeptide-2, and the mass ratio of acetyl hexapeptide-8, acetyl octapeptide-3, acetyl heptapeptide-4, palmitoyl tripeptide-5, and acetyl tetrapeptide-2 is 0.00002~0.005:0.00002~0.005:0.000008~0.002:0.000001~0.00025:0.000001~0.00025.

[0019] Preferably, the method for preparing the soluble collagen comprises the following steps:

[0020] Step 1: dehairing, washing, degreasing, and cleaning the pigskin to obtain cleaned pigskin;

[0021] Step 2: soaking the cleaned pigskin in an alkaline solution with a pH value of 11 to 13 for 5 to 10 minutes, taking it out and then soaking it in an acidic solution with a pH value of 4 to 5 for 5 to 10 minutes; after soaking, rinsing the pigskin with deionized water until the surface of the pigskin becomes neutral to obtain an intermediate;

[0022] Step 3: Soak the intermediate in an acetic acid solution with a pH value of 6 to 6.5, then add enzyme for enzymatic hydrolysis, inactivate the enzyme, filter, centrifuge, and collect the supernatant to obtain soluble collagen.

[0023] Preferably, the enzyme used in step 3 is selected from at least one of acidic protease, neutral protease, papain, trypsin and bromelain.

[0024] Preferably, the enzyme used in step 3 is a mixed enzyme of acid protease, trypsin and bromelain, and the mass ratio of acid protease, trypsin and bromelain is 2-3:1:3.

[0025] Preferably, the following components are included in parts by mass:

[0026]

[0027] In addition, the present application also discloses a preparation method for the above-mentioned instant anti-wrinkle and firming composition, which comprises mixing and dispersing small molecule peptides, snake venom peptides, soluble collagen, bosine, and ectoine to obtain the instant anti-wrinkle and firming composition.

[0028] In addition, the present application also discloses the application of the instant anti-wrinkle and firming composition for preparing cosmetics.

[0029] The beneficial effects of this application are:

[0030] In addition, the small molecule peptides and snake venom peptides in this application jointly stimulate the production of collagen, enhance the structural support of the skin, and the soluble collagen, bosine and ectoine provide deep moisturizing, improve the hydration state of the skin, and enhance the elasticity of the skin. As a result, the composition can not only improve the firmness and anti-wrinkle effects of the skin in a short period of time, but also achieve long-term skin improvement through continuous use. DETAILED DESCRIPTION

[0031] The present invention will be described clearly and completely below in conjunction with the examples of the present invention. In the description of the present invention, it should be noted that, where specific conditions are not specified in the examples, conventional conditions or conditions recommended by the manufacturer were used. Reagents or instruments used where the manufacturer is not specified are conventional products that can be purchased commercially.

[0032] Before presenting the examples and comparative examples, the following necessary descriptions of the raw materials involved in the examples and comparative examples are first made, as shown in Table 1:

[0033] Table 1: Raw material information

[0034]

[0035] It should be noted that, in the following examples and comparative examples, except for necessary explanations, the preparation method of soluble collagen is as follows:

[0036] Step 1: Purchase pig skin from the market, dehair it, and then rinse it three times with clean water. Then, degrease it and rinse it again three times with pure water to obtain the washed pig skin.

[0037] Step 2: Soaking the cleaned pigskin in a sodium hydroxide solution with a pH value of 12.5±0.5 for 8±1 minutes, then taking it out and soaking it in an acidic solution with a pH value of 4.5±0.5 for 8±1 minutes; after soaking, rinsing the pigskin with deionized water until the surface of the pigskin becomes neutral, thereby obtaining an intermediate;

[0038] Step 3: Soak the intermediate in an acetic acid solution with a pH value of 6.2±0.2, then add acid protease at a mass ratio of 100:3 between the intermediate and acid protease for enzymatic hydrolysis for 12 hours. After enzymatic hydrolysis, inactivate the enzyme, filter, centrifuge, and take the supernatant to obtain soluble collagen.

[0039] A method for preparing an instant anti-wrinkle and firming composition comprises the following steps: mixing small molecule peptides, snake venom peptides, soluble collagen, bosine and ectoine and stirring the mixture at a speed of 1000 rpm for 20 minutes to obtain the instant anti-wrinkle and firming composition.

[0040] Part 1: Verification of the firming ability of small molecule peptides

[0041] Examples 1-5, Comparative Examples 1-5

[0042] A composition, the formula is shown in Table 2:

[0043] Table 2: Formulas of Examples 1-5 and Comparative Examples 1-5

[0044]

[0045]

[0046]

[0047] Part II: Verification of the synergistic ability of small molecule peptides

[0048] Examples 6-15

[0049] An instant anti-wrinkle and firming composition, the formula of which is shown in Table 3:

[0050] Table 3: Formula of Examples 6-15

[0051]

[0052]

[0053] Part III Verification of Raw Material Addition Amount

[0054] Examples 16-18

[0055] An instant anti-wrinkle and firming composition, the formula of which is shown in Table 4:

[0056] Table 4: Formula of Examples 16-18

[0057] Group Example 16 Example 17 Example 18 Acetyl Hexapeptide-8 0.00005 copies 0.0125 parts 0.0002 copies Snake venom peptides 1 serving 0.00001 copies 0.001 copies Soluble collagen 0.000001 copies 0.1 part 0.0005 copies Bose 10.5 servings 0.001 copies 0.11 parts Ecdoin 0.001 copies 7 servings 0.11 parts

[0058] Part 4 Verification of Raw Materials Other than Small Molecule Peptides

[0059] Example 19

[0060] The method is basically the same as Example 1, except that the preparation method of soluble collagen is:

[0061] Step 3: Soak the intermediate in an acetic acid solution with a pH value of 6.2±0.2, then add trypsin at a mass ratio of 100:3 between the intermediate and trypsin for 12 hours. After enzymatic hydrolysis, inactivate the enzyme, filter, centrifuge, and take the supernatant to obtain soluble collagen.

[0062] Example 20

[0063] The method is basically the same as Example 1, except that the preparation method of soluble collagen is:

[0064] Step 3: Soak the intermediate in an acetic acid solution with a pH value of 6.2±0.2, then add bromelain at a mass ratio of 100:3 between the intermediate and bromelain for 12 hours, inactivate the enzyme, filter, centrifuge, and collect the supernatant to obtain soluble collagen.

[0065] Example 21

[0066] The method is basically the same as Example 1, except that the preparation method of soluble collagen is:

[0067] Step 3: Soaking the intermediate in an acetic acid solution with a pH of 6.2±0.2, followed by adding a mixture of acidic protease, trypsin, and bromelain in a mass ratio of 100:3 between the intermediate and acidic protease, and performing enzymatic hydrolysis for 12 hours. After enzymatic hydrolysis, the enzyme is inactivated, filtered, and centrifuged, and the supernatant is collected to obtain soluble collagen;

[0068] The mass ratio of the acidic protease, trypsin and bromelain in the mixture of the acidic protease, trypsin and bromelain is 2:1:3.

[0069] Part 5 Verification of interactions between raw materials

[0070] Comparative Examples 6-9

[0071] A composition, the formula is shown in Table 5:

[0072] Table 5: Comparative Examples 6-9 Formula

[0073]

[0074]

[0075] Performance testing:

[0076] Elastase inhibition test

[0077] 1.1 Experimental consumables

[0078] Elastase (porcine pancreas), BR, N-succinyl-L-alanyl-L-alanyl-L-alanine, 98%, BSA224S analytical balance, RT-6100 ELISA analyzer;

[0079] 1.2 Test methods

[0080] Method for designing samples and negative controls: The compositions prepared in the examples and comparative examples were diluted with pure water to a 10% solution by mass to serve as the sample group; at the same time, pure water was used as the negative control;

[0081] (2) Test operation steps

[0082] Set up sample group, sample background group, solvent group and solvent background group. Each group needs to set up 3 parallels. Add different reagent solutions into the 96-well plate, shake gently, incubate at 25℃ for 15min and 45min respectively, place it in the microplate reader, and measure the absorbance at 410nm.

[0083] (3) The calculation formula is shown in Formula 2:

[0084]

[0085] Where: A—is the absorbance of the reaction solution without sample and containing enzyme;

[0086] B—is the absorbance of the reaction solution without sample and enzyme;

[0087] C—is the absorbance of the reaction solution containing the sample and the enzyme;

[0088] D—is the absorbance of the reaction solution containing sample and without enzyme.

[0089] The results of the 15-minute elastin inhibition rate test are shown in Table 6:

[0090] Table 6: 15min elastase inhibition test results

[0091]

[0092] The results of the 45-minute elastin inhibition rate test are shown in Table 7:

[0093] Table 7: 45min elastin inhibition rate test results

[0094]

[0095]

[0096] Result Analysis

[0097] 1. As shown in Examples 1-5, Examples 16-18, and Examples 19-20, after minor adjustments and changes to the mass ratio of the raw materials in the composition, the type of small molecule peptide, and the method of extracting soluble collagen, the compositions prepared in the above Examples showed similar inhibition rates in both the 15-minute and 45-minute elastase tests. This indicates that minor adjustments to the mass ratio of the raw materials in the composition, the type of small molecule peptide, and the method of extracting soluble collagen have relatively little effect on the elastase inhibition ability of the composition.

[0098] 2. As shown in Examples 1 and 19-21, when acid protease, trypsin, and bromelain were used for mixed enzymatic hydrolysis during the preparation of soluble collagen, the resulting soluble collagen composition had superior elastase inhibition compared to that of Examples 1 and 19-20.

[0099] 3. As can be seen from Examples 1-5 and Comparative Examples 1-5, when small molecule peptides such as acetyl hexapeptide-8, acetyl octapeptide-3, acetyl heptapeptide-4, palmitoyl tripeptide-5, and acetyl tetrapeptide-2 are added to the composition, the anti-wrinkle effect of the composition is more advantageous;

[0100] Furthermore, further observation of Examples 6-15 shows that when a mixture of multiple small molecule peptides is used, the anti-wrinkle ability of the composition is more effective than when a single small molecule peptide is used;

[0101] Furthermore, by observing Examples 1-5 and 6, it can be seen that when any one of the five small molecule peptides is used alone in Examples 1-5, the best elastase inhibition rate of Examples 1-5 is only 31.761%. However, when the small molecule peptides are used in combination, the elastase inhibition rate of Example 6 reaches 35.584%, and its elastase inhibition ability is significantly higher than any of Examples 1-5. It can be seen that there is a synergistic effect between the small molecule peptides, which enables Example 6 to have good elastin inhibition ability.

[0102] 4. As shown in Examples 1-5 and Example 6, when a mixture of multiple small molecule peptides is used, the composition has better anti-wrinkle ability. In addition, when the small molecule peptide is a single type of small molecule peptide or a small molecule peptide similar to Example 6, the lack of snake venom peptide or soluble collagen in the composition shows that the anti-wrinkle effect of Comparative Example 6 is improved by approximately 3.246% relative to Comparative Example 7.

[0103] Comparative Example 8 is improved by about 1.166% relative to Comparative Example 9;

[0104] The performance of Example 6 is improved by about 6.655% compared with that of Example 1.

[0105] It can be seen that when soluble collagen and snake venom peptides exist at the same time, the synergistic effect between multiple small molecule peptides can be further enhanced. Therefore, it can be seen that there is a synergistic effect between soluble collagen, snake venom peptides and a mixture of multiple small molecule peptides.

Claims

1. An instant anti-wrinkle and firming composition, characterized in that: Calculated by mass, it includes the following components: The small molecule peptide is selected from at least one of acetyl hexapeptide-8, acetyl octapeptide-3, acetyl heptapeptide-4, palmitoyl tripeptide-5, and acetyl tetrapeptide-2.

2. The instant anti-wrinkle and firming composition according to claim 1, characterized in that: The small molecule peptide is a mixture of acetyl hexapeptide-8, acetyl octapeptide-3, acetyl heptapeptide-4, palmitoyl tripeptide-5, and acetyl tetrapeptide-2, and the mass ratio of acetyl hexapeptide-8, acetyl octapeptide-3, acetyl heptapeptide-4, palmitoyl tripeptide-5, and acetyl tetrapeptide-2 is 0.00002~0.005:0.00002~0.005:0.000008~0.002:0.000001~0.00025:0.000001~0.00025.

3. The instant anti-wrinkle and firming composition according to claim 1, characterized in that: The preparation method of the soluble collagen comprises the following steps: Step 1: dehairing, washing, degreasing, and cleaning the pigskin to obtain cleaned pigskin; Step 2: soaking the cleaned pigskin in an alkaline solution with a pH value of 11 to 13 for 5 to 10 minutes, taking it out and then soaking it in an acidic solution with a pH value of 4 to 5 for 5 to 10 minutes; after soaking, rinsing the pigskin with deionized water until the surface of the pigskin becomes neutral to obtain an intermediate; Step 3: Soak the intermediate in an acetic acid solution with a pH value of 6 to 6.5, then add enzyme for enzymolysis at a mass ratio of the intermediate to the enzyme of 100:2 to 4, inactivate the enzyme after enzymolysis, filter, centrifuge, and take the supernatant to obtain soluble collagen.

4. The instant anti-wrinkle and firming composition according to claim 3, characterized in that: The enzyme used in step 3 is specifically selected from at least one of acidic protease, neutral protease, papain, trypsin, and bromelain.

5. The instant anti-wrinkle and firming composition according to claim 4, characterized in that: The enzyme used in step 3 is specifically a mixed enzyme of acid protease, trypsin and bromelain, and the mass ratio of acid protease, trypsin and bromelain is 2-3:1:

3.

6. The instant anti-wrinkle and firming composition according to claim 1, characterized in that: Calculated by mass, it includes the following components:

7. A method for preparing the instant anti-wrinkle and firming composition according to any one of claims 1 to 6, characterized in that: Small molecule peptides, snake venom peptides, soluble collagen, bosine and ectoine are mixed and dispersed to obtain an instant anti-wrinkle and firming composition.

8. Use of the instant anti-wrinkle and firming composition according to any one of claims 1 to 6 in preparing cosmetic formulas.

Images