EGCG whitening composition with repair barrier efficacy and preparation method and application thereof

Abstract

The present application relates to a kind of EGCG whitening composition with the efficacy of repair barrier and its preparation method and application, belong to the field of cosmetics.The present application provides a kind of EGCG whitening composition, including the following mass parts of components: phenylethyl resorcinol 0.1-0.5 parts, lansan fruit leaf extract 0.2-2.0 parts and epigallocatechin gallate 0.1-0.5 parts.The present application will phenylethyl resorcinol, lansan fruit leaf extract and EGCG be collocated as EGCG whitening composition, lansan fruit leaf extract and EGCG can alleviate the irritability of phenylethyl resorcinol, realize the synergistic improvement of whitening and repair barrier.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to an EGCG whitening composition with barrier repair effect and a preparation method and application thereof. BACKGROUND

[0002] With the increasing demand for skin care from consumers, the field of cosmetics is constantly exploring innovative ingredients and formulations that can simultaneously repair the skin barrier and whiten the skin. Repairing the skin barrier is crucial for maintaining skin health, while whitening efficacy can improve uneven skin tone and hyperpigmentation problems. However, in existing cosmetics, ingredients for repairing the barrier and whitening are usually used separately, and commonly used whitening raw materials (such as phenylethyl resorcinol) often have some irritation. The market often has good whitening effect, but it needs to establish tolerance for use and is easy to damage the skin barrier, so as to not only cannot be used on consumers with sensitive skin or damaged skin barrier, but also makes the originally healthy skin state worse.

[0003] Therefore, developing a skin barrier repair composition with high whitening efficacy is a research hotspot in the field of cosmetics and an urgent need for consumers today, hoping to achieve safe and high-efficiency whitening. SUMMARY

[0004] The purpose of the present application is to overcome the shortcomings of the prior art and provide an EGCG whitening composition with skin barrier repair effect and a preparation method and application thereof.

[0005] To achieve the above-mentioned purpose, the technical solution adopted by the present application is as follows:

[0006] In a first aspect, the present application provides an EGCG whitening composition with barrier repair effect, comprising the following components by mass: phenylethyl resorcinol 0.1-0.5 parts, Laium domesticum leaf extract 0.2-2.0 parts and epigallocatechin gallate 0.1-0.5 parts.

[0007] Phenylethyl resorcinol (commonly known as 377) is a cosmetic raw material with significant whitening effect, but it has high irritation. If the addition concentration is high, the user needs to establish tolerance before achieving daily whitening effect, and people with sensitive skin have difficulty in establishing tolerance to normally use cosmetics containing this raw material.

[0008] Laium domesticum leaf extract is a natural product extracted from Laium domesticum leaves, which has relatively high safety and certain whitening effect.

[0009] Epigallocatechin gallate (EGCG) is the most active ingredient in tea polyphenols, which is extracted from natural plants such as tea leaves, relatively safe, and meets the needs of consumers for natural cosmetic ingredients. EGCG can scavenge various free radicals, absorb part of ultraviolet rays, reduce the damage of ultraviolet rays to the skin, and reduce the oxidative damage and inflammatory response induced by ultraviolet rays through antioxidant effect.

[0010] In the present application, phenylethyl resorcinol, Lansan fruit leaf extract and EGCG are matched as an EGCG whitening composition. The Lansan fruit leaf extract and EGCG can alleviate the irritation of phenylethyl resorcinol, and realize the synergistic improvement of whitening and barrier repair.

[0011] As a preferred embodiment of the EGCG whitening composition of the present application, the following components are included: phenylethyl resorcinol 0.15-0.3 parts, Lansan fruit leaf extract 0.5-1.0 parts and epigallocatechin gallate 0.15-0.3 parts. In the preferred mass ratio range, the present application realizes through experiments that the EGCG whitening composition in the specific mass range has excellent whitening and barrier repair efficacy.

[0012] As a preferred embodiment of the EGCG whitening composition of the present application, the following components are included: phenylethyl resorcinol 0.3 parts, Lansan fruit leaf extract 1.0 parts and epigallocatechin gallate 0.3 parts. The present application realizes through experiments that the EGCG whitening composition in the specific mass range has excellent whitening and barrier repair efficacy.

[0013] As a preferred embodiment of the EGCG whitening composition of the present application, the following components are included: phenylethyl resorcinol 0.5 parts, Lansan fruit leaf extract 2.0 parts and epigallocatechin gallate 0.5 parts. The present application realizes through experiments that the EGCG whitening composition in the specific mass range has the most excellent whitening and barrier repair efficacy.

[0014] As a preferred embodiment of the EGCG whitening composition of the present application, the mass ratio of the sum of phenylethyl resorcinol and epigallocatechin gallate to Lansan fruit leaf extract is (phenylethyl resorcinol + epigallocatechin gallate) : Lansan fruit leaf extract = (0.1-2) : 1. The present application realizes through experiments that the sum of phenylethyl resorcinol and epigallocatechin gallate and Lansan fruit leaf extract in the specific ratio range can realize more excellent whitening and barrier repair efficacy.

[0015] As a preferred embodiment of the EGCG whitening composition of the present application, the mass ratio of the sum of the phenylethyl resorcinol and epigallocatechin gallate to the extract of Vaccinium vitis-idaea is (phenylethyl resorcinol + epigallocatechin gallate) : extract of Vaccinium vitis-idaea = (0.1-1) : 1. The present application finds through experiments that the sum of the phenylethyl resorcinol and epigallocatechin gallate and the extract of Vaccinium vitis-idaea can further improve the whitening and barrier repair effects of the EGCG whitening composition in a specific ratio range.

[0016] As a preferred embodiment of the EGCG whitening composition of the present application, the mass ratio of the sum of the phenylethyl resorcinol and epigallocatechin gallate to the extract of Vaccinium vitis-idaea is (phenylethyl resorcinol + epigallocatechin gallate) : extract of Vaccinium vitis-idaea = 0.5 : 1.

[0017] In a second aspect, the present application provides a preparation method of the above-mentioned EGCG whitening composition, wherein phenylethyl resorcinol, the extract of Vaccinium vitis-idaea and epigallocatechin gallate are mixed at 50-70℃ to obtain the EGCG whitening composition.

[0018] In a third aspect, the present application provides an application of the above-mentioned EGCG whitening composition in the preparation of cosmetics.

[0019] The present application proves through experiments that the EGCG whitening composition has excellent whitening and barrier repair effects on the skin and can be applied in various types of cosmetics.

[0020] As a preferred embodiment of the application, the dosage form of the cosmetic includes at least one of the following: cream, emulsion, water agent, gel, oil agent, powder, block powder or solid, patch, film and lyophilized product.

[0021] In a fourth aspect, the present application provides an emulsion with barrier repair and whitening effects, which comprises 0.5-3 parts by mass of the above-mentioned EGCG whitening composition, 2-4 parts by mass of a stabilizer, 0.4-0.6 parts by mass of a preservative, 65-85 parts by mass of water, 0.7-1.5 parts by mass of an emulsifier and 9.85-18.2 parts by mass of a humectant; the emulsifier comprises emulsifier A and emulsifier B, and the humectant comprises aqueous humectant and oily humectant.

[0022] The emulsifier A in the emulsion comprises at least one of polysorbate-80, Span 80 and Span 85, and the emulsifier B in the emulsion comprises triethanolamine and / or fatty alcohol polyoxyethylene ether. The present application proves by experiments that the EGCG whitening composition not only has excellent whitening and skin barrier repairing effects, but also has certain stability, and can improve the stability of the emulsion and reduce the yellowing phenomenon of benzene ethyl resorcinol in the formula application when applied in the emulsion cosmetic.

[0023] As a preferred embodiment of the emulsion of the present application, the emulsion comprises 1.25 parts by mass of the above-mentioned EGCG whitening composition, 3 parts by mass of a stabilizer, 0.5 parts by mass of a preservative, 65-85 parts by mass of water, 13.7 parts by mass of an emulsifier and 1.1 parts by mass of a humectant.

[0024] As a preferred embodiment of the emulsion of the present application, the stabilizer in the emulsion comprises caprylic acid / capric acid triglyceride and / or tocopheryl acetate.

[0025] As a preferred embodiment of the emulsion of the present application, the preservative in the emulsion comprises at least one of phenoxyethanol, hydroxybenzoic acid ester and sorbic acid.

[0026] As a preferred embodiment of the emulsion of the present application, the oily humectant in the emulsion comprises at least one of isononyl isononanoate, jojoba oil and lecithin, and the aqueous humectant in the emulsion comprises at least one of glycerol, 1,3-butanediol and allantoin.

[0027] As a preferred embodiment of the emulsion of the present application, the emulsifier A in the emulsion contains polysorbate-80, the emulsifier B in the emulsion contains triethanolamine, and the mass ratio of the polysorbate-80 to the triethanolamine is polysorbate-80: triethanolamine = (0.5-1.0):(0.2-0.5).

[0028] As a preferred embodiment of the emulsion of the present application, the mass ratio of the polysorbate-80 to the triethanolamine is polysorbate-80: triethanolamine = 0.8:0.3.

[0029] As a preferred embodiment of the emulsion of the present application, the oily humectant in the emulsion comprises 2.0-4.0 parts by mass of isononyl isononanoate, 1.5-3.0 parts by mass of jojoba oil and 0.2-0.8 parts by mass of lecithin, and the aqueous humectant in the emulsion comprises 4.0-6.0 parts by mass of glycerol, 2.0-4.0 parts by mass of 1,3-butanediol and 0.15-0.4 parts by mass of allantoin.

[0030] As a preferred embodiment of the emulsion of the present application, the oily moisturizer in the emulsion comprises 3.0 parts by mass of isononyl isononanoate, 2.0 parts by mass of jojoba oil, and 0.5 parts by mass of lecithin, and the aqueous moisturizer in the emulsion comprises 5.0 parts by mass of glycerin, 3.0 parts by mass of 1,3-butanediol, and 0.2 parts by mass of allantoin.

[0031] As a preferred embodiment of the emulsion of the present application, the emulsion further comprises 0.1 parts by mass of a fragrance.

[0032] In a fifth aspect, the present application provides a preparation method of the above-mentioned emulsion, comprising the following steps:

[0033] (1) mixing the oily moisturizer, the emulsifier B, and the stabilizer at 75-80°C to obtain an oil phase;

[0034] (2) mixing the aqueous moisturizer and water at 23-27°C to obtain an aqueous phase;

[0035] (3) adding the aqueous phase obtained in step (2) into the oil phase obtained in step (1) and homogenizing at 75-80°C for 10-15 min, and then adding the emulsifier B when the temperature drops to 50-55°C to obtain a mixed solution;

[0036] (4) adding the above-mentioned EGCG whitening composition when the mixed solution obtained in step (3) is cooled to 40-50°C, and adding the preservative when the temperature drops to 30-40°C, and then uniformly mixing and cooling to 23-27°C to obtain the product, which is the emulsion.

[0037] As a preferred embodiment of the preparation method of the present application, in step (4), the EGCG whitening composition is in a liquid state.

[0038] As a preferred embodiment of the preparation method of the present application, when the emulsion contains a fragrance, step (4) is performed as follows:

[0039] (4) adding the preservative and the fragrance when the mixed solution obtained in step (3) is cooled to 30-40°C, and then uniformly mixing and cooling to 23-27°C to obtain the product, which is the emulsion.

[0040] Compared with the prior art, the present application has the following advantages:

[0041] (1) The present application utilizes the antioxidant properties of EGCG, and combines the lanza fruit leaf extract and the phenylethyl resorcinol to form an EGCG whitening composition, which can reduce the irritation of the phenylethyl resorcinol while maintaining excellent whitening performance, and realizes the synergistic improvement of the dual performance of whitening and barrier repair.

[0042] (2) The present application prepares the EGCG whitening composition with other cosmetic auxiliaries (such as moisturizing agent, emulsifying agent, stabilizer, preservative, etc.) into a cosmetic with whitening and barrier repair effects, and finds that the EGCG whitening composition can improve the stability of the cosmetic and reduce the yellowing phenomenon of hydroxybenzene in the formula application in addition to the excellent whitening and barrier repair effects. BRIEF DESCRIPTION OF DRAWINGS

[0043] Figure 1 A tyrosinase enzyme inhibition rate detection result graph of different emulsions in effect example 1 of the present application;

[0044] Figure 2 A morphology graph of the emulsion obtained in example 9 in effect example 2 of the present application. DETAILED DESCRIPTION

[0045] In order to better illustrate the purpose, technical scheme and advantages of the present application, the present application will be further described below in combination with specific examples.

[0046] Unless otherwise specified, other materials, reagents, etc. used in the examples, comparative examples and effect examples can be obtained from commercial channels.

[0047] The Lansium domesticum fruit leaf extract in the following examples and comparative examples is purchased from Foshan Xian'an Chemical Trade Co., Ltd., and the trade name is

[0048] 0.05 mol / L PBS (pH = 6.8), 50 mL of methanol and 50 mL of ethanol are mixed to prepare, the methanol is 0.05 mol / L Na2HPO4 solution, and the ethanol is 0.05 mol / L KH2PO4 solution.

[0049] The tyrosinase solution is a 100 U / mL tyrosinase PBS solution, and the PBS is 0.05 mol / L PBS (pH = 6.8), which is prepared immediately before use.

[0050] The levodopa solution is a 1 mg / mL levodopa PBS solution, and the PBS is 0.05 mol / L PBS (pH = 6.8), which is stored in the dark.

[0051] Examples 1-6 and comparative examples 1-4

[0052] Examples 1-6 and comparative examples 1-4 respectively provide an EGCG whitening composition with barrier repair effect and a preparation method thereof, the components and amounts of the EGCG whitening composition are shown in Table 1, and the preparation method is to mix hydroxybenzene, Lansium domesticum fruit leaf extract and epigallocatechin gallate at 75-80°C to obtain the EGCG whitening composition.

[0053] Table 1 Components and their amounts (mass parts) of different EGCG whitening compositions

[0054]

[0055]

[0056] Comparative Example 5

[0057] Comparative Example 5 provides an EGCG whitening composition with barrier repair efficacy and a preparation method thereof, the components and their amounts of the EGCG whitening composition are similar to those of Example 1, except that the benzene ethyl resorcinol is replaced by arbutin, and the rest of the components and their amounts remain unchanged, and the preparation method is the same as that of Example 1.

[0058] Comparative Example 6

[0059] Comparative Example 6 provides an EGCG whitening composition with barrier repair efficacy and a preparation method thereof, the components and their amounts of the EGCG whitening composition are similar to those of Example 1, except that the EGCG is replaced by glycyrrhizin, and the rest of the components and their amounts remain unchanged.

[0060] Examples 7-11 and Comparative Examples 7-8

[0061] Examples 7-11 and Comparative Examples 7-8 respectively provide an emulsion with barrier repair and whitening efficacy and a preparation method thereof, the components and their amounts of the emulsion are shown in Table 2, and the preparation method comprises the following steps:

[0062] (1) Mix the oily moisturizer, emulsifier A, and stabilizer at 76-82°C to obtain an oil phase;

[0063] (2) Mix the aqueous moisturizer and water at 23-27°C to obtain an aqueous phase;

[0064] (3) Add the aqueous phase obtained in step (2) to the oil phase obtained in step (1) and homogenize at 75-80°C for 10-15 min, then add emulsifier B when the temperature drops to 50-55°C to obtain a mixture;

[0065] (4) When the mixture obtained in step (3) is cooled to 45-50°C, add the EGCG whitening composition, and when it is cooled to 30-40°C, add the preservative and fragrance, mix well, and cool to 23-27°C. The product obtained is the emulsion.

[0066] Table 2 Components and their amounts (mass parts) of different emulsions

[0067]

[0068] Comparative Example 9

[0069] Comparative Example 9 provides an emulsion having the effects of repairing barrier and whitening and a preparation method thereof, the components and amounts of the emulsion are similar to those of Example 7, except that the EGCG whitening composition in the emulsion obtained in Example 7 is replaced by the EGCG whitening composition obtained in Comparative Example 5, the other components and amounts thereof remain unchanged, and the preparation method is the same as that of Example 7.

[0070] Example 1

[0071] The EGCG whitening compositions obtained in Examples 1-6 and Comparative Examples 1-6 were tested for whitening and barrier repair effects, and the specific scheme is as follows:

[0072] 1. Whitening effect.

[0073] The test solution was prepared according to Table 3, and the test sample, 0.05M PBS buffer (pH 6.8), and tyrosinase solution were sequentially added to a test tube; the test tube was placed in a 37°C water bath for 10 min; then, 2 mL of dopa solution was added, and the absorbance was measured at 475 nm after 5 min of reaction, the absolute difference between two independent measurement results obtained under repeatability conditions should not exceed 10% of the arithmetic mean value, and the tyrosinase inhibition rate (%) was calculated according to Formula (1), and the results are shown in Table 4 and Figure 1 .

[0074] Table 3 Composition of reaction solution (mL)

[0075]

[0076] Tyrosinase inhibition rate (%) = [1-(OD C - OD D ) / (OD A - OD B )] x 100% - Formula (1)

[0077] In the above formula, OD A is the absorbance value of the enzyme solution control group; OD B is the absorbance value of the enzyme solution control blank group; OD C is the absorbance value of the sample group; and OD D is the absorbance value of the sample group blank control.

[0078] Table 4 Tyrosinase inhibition rate detection results of different EGCG whitening compositions

[0079]

[0080]

[0081] From Table 4 and Figure 1As shown, the tyrosinase inhibition rates of the examples are higher than those of the comparative examples, indicating that the EGCG whitening composition of the present application has a relatively excellent whitening effect, and the tyrosinase inhibition rate of Example 2 is the highest, indicating that the EGCG whitening composition of Example 2 has the most excellent whitening effect.

[0082] As can be seen from Examples 1-5, as the use amount of phenylethyl resorcinol and EGCG increases, the tyrosinase inhibition rate of the EGCG whitening composition also increases, but when the mass ratio of phenylethyl resorcinol and EGCG to the extract of Lansium domesticum leaves exceeds 2:1, the tyrosinase inhibition rate of the EGCG whitening composition instead shows a downward trend, indicating that when the mass ratio of phenylethyl resorcinol and EGCG to the extract of Lansium domesticum leaves is (0.1-2):1, the tyrosinase inhibition rate of the EGCG whitening composition is more prominent.

[0083] Example 2 has a higher tyrosinase inhibition rate than Comparative Examples 1-3, and the sum of the tyrosinase inhibition rates of Comparative Examples 1-3 (66.85%) is less than that of Example 2; Example 2 has a higher tyrosinase inhibition rate than Comparative Examples 4-6, and if the mass ratio of phenylethyl resorcinol and EGCG to the extract of Lansium domesticum leaves is not within the range of (0.1-3):1, the tyrosinase inhibition rate of the EGCG whitening composition will decrease, and if phenylethyl resorcinol or EGCG is replaced by a similar active ingredient, the extract of Lansium domesticum leaves cannot synergize with the active ingredient, indicating that only when phenylethyl resorcinol, EGCG and the extract of Lansium domesticum leaves are combined into the EGCG whitening composition can the whitening effect be synergistically improved, and the mass ratio of phenylethyl resorcinol and EGCG to the extract of Lansium domesticum leaves needs to be within the range of (0.1-3):1 to achieve a more excellent whitening effect.

[0084] 2. Repair performance test

[0085] Cell migration experiment

[0086] Principle of experiment:

[0087] Cell migration ability directly reflects the level of cell activity. If cells can proliferate and migrate rapidly, they can form a protective mechanism as soon as possible in response to external stimuli, thereby repairing damaged cells. The cell scratch (repair) method is a simple method for determining the migration and repair ability of cells. It is similar to an in vitro wound healing model. A microgun head or other hard object is used to draw a line in the central area of the cells growing on the monolayer of cells cultured in a petri dish or plate. The cells in the central area are removed, and then the cells are cultured for a certain period of time. Then the cell culture plate is taken out, and whether the peripheral cells grow (repair) to the central scratch area is observed to determine the repair ability of the cells. The experiment usually needs to set up a normal control group and an experimental group. The experimental group is a group with certain treatment factors or drugs, exogenous genes, etc. The migration and repair ability of cells in different groups can be determined by the repair ability of cells in the scratch area.

[0088] Sample: (1) negative control: 10% serum-containing DMEM culture solution; (2) sample group: EGCG whitening compositions of Examples 1-6 and EGCG whitening compositions of Comparative Examples 1-6, prepared into a 2% solution using 10% serum-containing DMEM culture solution as a solvent; (3) positive control: 10% epidermal growth factor (EGF) solution prepared using 10% serum-containing DMEM culture solution as a solvent.

[0089] Experimental procedure: HaCaT human keratinocytes were inoculated in a 12-well plate (100,000 cells / well) and cultured in a culture box at 37°C for 24 h. After the end of the culture, the original culture solution in the wells was discarded, and two longitudinal and two horizontal scratches were made in each well using a gun head. In the negative control group, 100 μL of 1% serum-containing DMEM culture solution was added, in the sample group, 100 μL of the prepared sample solution was added to each well, and in the positive control group, 100 μL of 1% EGF solution was added to each well. The culture was continued for 18 h. At the 0th and 18th time points, samples were taken, photographed, and 3 random areas were selected to calculate the average intercellular scratch area.

[0090] The cell migration rate (wound healing rate) was calculated by the average intercellular scratch area at the 0th and 18th hours. The repair effect of the sample was expressed by the cell migration rate (wound healing rate). The greater the cell migration rate (wound healing rate), the better the repair effect.

[0091] Cell migration rate (wound healing rate) % = (0h scratch area - 18h scratch area) / 0h scratch area

[0092] The results are shown in Table 5.

[0093] Table 5: Cell repair detection results of different EGCG whitening compositions

[0094]

[0095]

[0096] As shown in Table 5, the EGCG compositions of Examples 1-6 all have good cell repair ability, and Example 1 has the best effect, which shows that with the addition of Lansat fruit leaf extract, good repair effect can be achieved without high concentration of EGCG, and with the decrease of Lansat fruit leaf extract, the cell repair ability will decrease.

[0097] Compared with the examples, the cell migration rate of Comparative Examples 1-3 is 51.14%, which is lower than that of the examples, which shows that the EGCG composition of the present application is not a simple superposition of phenylethyl, EGCG and Lansat fruit leaf extract, and the combination of the three has the effect of synergistically improving repair.

[0098] Example 2

[0099] The emulsions obtained in Examples 7-11 and Comparative Examples 7-9 were tested for stability, and the emulsions were placed in room temperature (25±2℃) and high temperature (45℃) environments for 45 days. The emulsion state was observed and photographed at 7 days and 45 days, and the state of Example 9 at room temperature is shown in Figure 2 , and the results of the rest are shown in Table 6.

[0100] Table 6 Stability test results of different emulsions

[0101]

[0102]

[0103] As Figure 2 shown, the emulsion of Example 9 is white or milky white, and the texture is slightly thick. As shown in Table 6, the emulsion of the example has higher stability, and only a slight yellowing phenomenon occurs after being stored at high temperature for 45 days, and the emulsion texture is stable, while the emulsion of Comparative Examples 8-9 appears yellow after being stored at room temperature for 45 days, and the texture becomes thin after being stored at high temperature for 45 days, which shows that the use of EGCG whitening composition in excess or replaced by the EGCG whitening composition obtained in Comparative Example 5 will affect the stability of the emulsion.

[0104] Example 3

[0105] The emulsions obtained in Examples 7-11 and Comparative Examples 7-9 were tested for whitening and repair barrier efficacy, and the specific scheme is as follows:

[0106] 1. Whitening effect.

[0107] The test was carried out according to the whitening effect test method in Example 1, and the results are shown in Table 7.

[0108] Table 7 Tyrosinase inhibition rate test results of different emulsions

[0109] Group Tyrosinase inhibition rate (%) Example 7 74.34±2.56 Example 8 68.35±3.68 Example 9 78.45±2.76 Example 10 72.56±1.34 Example 11 69.45±4.27 Comparative Example 7 11.31±2.45 Comparative Example 8 43.23±1.46 Comparative Example 9 41.31±3.83

[0110] As shown in Table 7, the emulsions of the examples all have good tyrosinase inhibition rates, and the tyrosinase inhibition rate of Example 9 is the highest, indicating that the EGCG whitening composition of the present application has good whitening effect when applied in cosmetics. The tyrosinase inhibition rate of Example 9 is better than that of Comparative Examples 7-9, indicating that if the EGCG whitening composition is not added, the amount of the EGCG whitening composition is not appropriate, or the EGCG whitening composition with poor whitening effect is applied in cosmetics, the good whitening effect cannot be achieved.

[0111] 2, repair barrier effect.

[0112] 40 volunteers were recruited and divided into 8 groups, 5 people in each group, and the emulsions obtained from Examples 7-11 and Comparative Examples 7-9 were used respectively, and the transepidermal water loss rate (TEWL) of the volunteers' skin was tested by a skin moisture loss tester TewameterR TM 300.

[0113] The screening indicators of the volunteers include: healthy women, aged 30-60 years old, sensitive skin (lactic acid stinging reaction positive), dry and red skin on the face, poor skin barrier function in the test area (TEWL≥15 g / m 2 / h), can read and understand all the contents of the informed consent form, voluntarily sign the informed consent form, agree not to use any cosmetics, drugs and health products that may affect the results during the test period, do not participate in any other clinical trials in other research centers during the test period, and have not participated in any other clinical studies in the past month.

[0114] Ensure that the volunteers have not used antihistamines in the past week or immunosuppressants in the past month, have not used any anti-inflammatory drugs in the test area in the past two months, do not have inflammatory skin diseases or have inflammatory skin diseases but have been clinically cured, do not have other chronic respiratory diseases such as diabetes or asthma, are not in the lactation, pregnancy or family planning period, have not undergone bilateral mastectomy or bilateral axillary lymph node dissection, have not received anti-cancer chemotherapy in the past 6 months, are not allergic, have not undergone dermatological treatment, have not taken hydroxy acid, whitening and anti-aging drugs in the past month, and the test area has no large area of birthmarks, scratches, vitiligo, pigmented nevi, scar nodules and other skin manifestations that affect the test.

[0115] During the test, the volunteers should pay attention to the following: keep the same life and diet habits as before the test, prohibit treatment on the tested part, prohibit the use of other skin care products and other products affecting the test, and keep the daily necessities unchanged as before the test, and prohibit replacement.

[0116] Test the TEWL value of the volunteers before starting to use the sample (the value is recorded as W0), and the volunteers apply 1-2 g of the sample evenly on the face, gently massage and absorb, use once a day, and test the TEWL value after 4 weeks of continuous use (the value is recorded as W4). Before testing the TEWL value, wash the face with clean water, sit still for 30 min in a constant temperature and humidity environment, and then start the test. The TEWL detection results are shown in Table 8.

[0117] Table 8 TEWL detection results of different emulsions

[0118]

[0119]

[0120] As shown in Table 8, the TEWL change rate of the emulsion obtained in the examples is high, and the change rate of Example 9 is the highest, indicating that the EGCG whitening composition of the present application has good barrier repair effect when applied in cosmetics. The TEWL change rate of Example 9 is better than that of Comparative Examples 7-9, indicating that if the EGCG whitening composition is not added, the amount of the EGCG whitening composition is not appropriate, or the EGCG whitening composition with poor barrier repair effect is applied in cosmetics, the barrier repair effect cannot be good.

[0121] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application and not to limit the protection scope of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced equivalently without departing from the essence and scope of the technical solutions of the present application.

Claims

1. An EGCG whitening composition having a repair barrier efficacy, characterized in that, The components include the following quality parts: phenylethyl resorcinol 0.1-0.5 parts, Lansan fruit leaf extract 0.2-2.0 parts and epigallocatechin gallate 0.1-0.5 parts; The mass ratio of the sum of the phenylethyl resorcinol and epigallocatechin gallate to the Lansan fruit leaf extract is (phenylethyl resorcinol + epigallocatechin gallate): Lansan fruit leaf extract = (0.1-2):

1.

2. The EGCG whitening composition of claim 1, wherein The mass ratio of the sum of the phenylethyl resorcinol and epigallocatechin gallate to the Lansan fruit leaf extract is (phenylethyl resorcinol + epigallocatechin gallate): Lansan fruit leaf extract = (0.1-1):

1.

3. The method for preparing the EGCG whitening composition according to any one of claims 1-2, characterized in that, The phenylethyl resorcinol, Lansan fruit leaf extract and epigallocatechin gallate are mixed at 75-80°C to obtain the EGCG whitening composition.

4. Use of the EGCG whitening composition according to any one of claims 1-2 in the preparation of a cosmetic.

5. The use according to claim 4, wherein the compound is ###0002### The dosage form of the cosmetic includes at least one of cream, emulsion, water, gel, oil, powder, block powder or solid, patch, film and lyophilized.

6. An emulsion having a repair barrier and whitening efficacy, characterized in that, The emulsion includes 0.3-3 mass parts of the EGCG whitening composition according to any one of claims 1-2, 2-4 mass parts of a stabilizer, 0.4-0.6 mass parts of a preservative, 65-85 mass parts of water, 0.7-1.5 mass parts of an emulsifier and 9.85-18.2 mass parts of a humectant; the emulsifier includes emulsifier A and emulsifier B, and the humectant includes aqueous humectant and oily humectant; The emulsifier A in the emulsion includes at least one of polysorbate-80, Span 80 and Span 85, and the emulsifier B in the emulsion includes triethanolamine and / or fatty alcohol polyoxyethylene ether.

7. The emulsion of claim 6, wherein, It includes at least one of the following (I)-(III): (I) The stabilizer in the emulsion includes caprylic / capric triglyceride and / or tocopheryl acetate; (II) The preservative in the emulsion includes at least one of phenoxyethanol, methylparaben and sorbic acid; (III) The oily humectant in the emulsion includes at least one of isononyl isononanoate, jojoba oil and lecithin, and the aqueous humectant in the emulsion includes at least one of glycerol, 1,3-butanediol and allantoin.

8. The emulsion of claim 7, wherein, It includes at least one of the following (IV)-(V): (IV) The emulsifier A in the emulsion contains polysorbate-80, the emulsifier B in the emulsion contains triethanolamine, and the mass ratio of the polysorbate-80 to the triethanolamine is polysorbate-80: triethanolamine = (0.5-1.0):(0.2-0.5); (V) The oily humectant in the emulsion includes 2.0-4.0 mass parts of isononyl isononanoate, 1.5-3.0 mass parts of jojoba oil and 0.2-0.8 mass parts of lecithin, and the aqueous humectant in the emulsion includes 4.0-6.0 mass parts of glycerol, 2.0-4.0 mass parts of 1,3-butanediol and 0.15-0.4 mass parts of allantoin.

9. A process for the preparation of an emulsion as claimed in any of claims 6 to 8, characterised in that, It includes the following steps: (1) Mix the oily moisturizer, emulsifier A and stabilizer at 75-80°C to obtain an oil phase; (2) Mix the aqueous moisturizer and water at 23-27°C to obtain an aqueous phase; (3) Add the aqueous phase obtained in step (2) to the oil phase obtained in step (1) and homogenize at 75-80°C for 10-15 min. When the temperature drops to 50-55°C, add emulsifier B to obtain a mixture; (4) When the mixture obtained in step (3) is cooled to 45-50°C, add the EGCG whitening composition according to any one of claims 1-2, and when cooled to 30-40°C, add a preservative, mix well, and cool to 23-27°C. The product obtained is the emulsion.

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