Composition with soothing effect as well as preparation method and application thereof in cosmetics

Abstract

The invention relates to the technical field of cosmetics, in particular to a composition with a soothing effect, a preparation method of the composition and application of the composition in cosmetics. The composition with the soothing effect is prepared from the following raw materials in percentage by mass: 10 to 25 percent of sophora flower bud extracting solution, 10 to 20 percent of kadsura longepedunculata extracting solution, 10 to 20 percent of radix astragali preparata extracting solution, 1 to 5 percent of semen coicis extracting solution, 1 to 5 percent of trollflower extracting solution, 20 to 40 percent of polyhydric alcohol and the balance of purified water. The sophora flower bud extracting solution is matched with the kadsura longepedunculata extracting solution, the radix astragali preparata extracting solution, the semen coicis extracting solution and the trollius chinensis bunge extracting solution to form a multi-target relieving mechanism, and the effects of inhibiting release of inflammatory factors and relieving are jointly achieved.

Description

Technical Field

[0001] The present application relates to the technical field of cosmetics, and in particular to a composition with a soothing effect, a preparation method thereof, and an application in cosmetics. Background Art

[0002] Environmental factors, lifestyle habits, genetics, hormone levels, and psychological factors can all contribute to skin sensitivity. Impaired skin barrier function is one of the primary causes of sensitive skin: thinning of the stratum corneum, decreased ceramides, and increased neutral lipids can all compromise the integrity of the skin barrier, increasing the skin's reactivity to irritants. Sensitive skin is often accompanied by immune and inflammatory responses, and inflammatory mediators produced by immune cells can exacerbate skin sensitivity.

[0003] When allergens enter the skin, they stimulate the body's immune system. Langerhans cells in the skin process the allergens, transmitting their information to T cells, which in turn activate B cells to produce IgE antibodies. These antibodies bind to mast cells and basophils in the skin, putting the body into a sensitized state. When the same allergen comes into contact with the skin again, it binds to the IgE antibodies on the surface of mast cells and basophils, causing these cells to release inflammatory mediators such as histamine. Histamine causes local blood vessels to dilate and increase permeability, causing symptoms such as skin redness, swelling, and itching. In addition, mast cells can also release platelet-activating factor and cellular inflammatory factors, further activating inflammatory cells and amplifying the inflammatory response.

[0004] Commonly used anti-allergic agents in the industry primarily rely on single plant extracts or combinations of a small number of ingredients, which can only alleviate skin inflammation or soothe sensitivity from a single dimension, and are unable to achieve comprehensive efficacy across multiple targets and pathways. For example, some products alleviate inflammation solely by inhibiting the release of inflammatory factors, while neglecting skin barrier repair and immune regulation. Other products' soothing effects only last for a short time, making it difficult to achieve long-term improvements in skin health. Summary of the Invention

[0005] In order to improve the problem that commonly used anti-allergic agents mainly rely on a single plant extract or a combination of a small number of ingredients, can only relieve skin inflammation or soothe sensitivity from a certain dimension, and are difficult to exert comprehensive efficacy from multiple targets and multiple pathways, the present application provides a composition with a soothing effect and a preparation method thereof.

[0006] In a first aspect, the present application provides a composition with a soothing effect, which adopts the following technical solution: A soothing composition comprising the following raw materials in percentage by weight: 10-25% of Sophora japonica bud extract, 10-20% of Schisandra chinensis fruit extract, 10-20% of Astragalus membranaceus extract, 1-5% of Coix lachryma-jobi seed extract, 1-5% of Trollius chinensis extract, 20-40% of polyol, and the balance being purified water; The mass concentrations of the Sophora japonica bud extract, Schisandra chinensis chinensis extract, Astragalus membranaceus extract, Coix lachryma-jobi seed extract, and Trollius truncatum extract are all 4-6 wt %.

[0007] By adopting the above technical solution, the present application forms a multi-target soothing mechanism by using Sophora japonica bud extract in combination with Schisandra chinensis extract, Astragalus membranaceus extract, Coix seed extract, and Trollius chinensis extract to jointly achieve the effects of inhibiting the release of inflammatory factors and soothing: Sophora japonica extract is rich in flavonoids such as rutin, which can inhibit the production of inflammatory factors, inhibit mast cell degranulation, and reduce the release of histamine. At the same time, it regulates the Th1 / Th2 balance and reduces the level of Th2 cytokines, thereby achieving an anti-allergic effect. In addition, Sophora japonica extract also reduces the damage of free radicals to skin cells through antioxidant effects. Schisandra chinensis extract contains a variety of organic acids and volatile oils, which can regulate the skin's immune response and reduce the release of inflammatory mediators, thereby alleviating skin redness, swelling and itching; Astragalus extract is rich in astragalus polysaccharides and flavonoids, which have immunomodulatory effects. It can enhance skin immunity and promote the repair of skin barrier, thereby improving skin tolerance. Coix seed extract contains ingredients such as coixol, which has antioxidant and whitening effects, can inhibit the production of melanin, and reduce inflammatory reactions; Nasturtium extract can reduce the release of inflammatory mediators, reduce the risk of skin infection, and help other ingredients penetrate into the skin better, effectively alleviating skin sensitivity symptoms.

[0008] This application also achieves a synergistic effect by scientifically combining multiple plant extracts: the anti-inflammatory effect of Sophora japonica extract combined with the immunomodulatory effect of Schisandra chinensis extract can more comprehensively relieve skin inflammation; the anti-inflammatory and anti-allergic effects of Sophora japonica extract are enhanced by Schisandra chinensis and Astragalus membranaceus extracts, the moisturizing effect of Coix seed extract reduces skin dryness and itching, and the guiding effect of Nasturtium flower extract improves the penetration and absorption of other ingredients, thereby achieving better inhibition of inflammatory factor release and soothing effects.

[0009] Preferably, the mass percentage of the Schisandra chinensis extract is 12-17%.

[0010] By adopting the above technical solution, when the content of Schisandra chinensis extract is too low, the reinforcing effect of Schisandra chinensis extract on the soothing effect of Astragalus membranaceus extract is weak; when the content of Schisandra chinensis extract is too high, too much organic acid will be introduced into the composition, which may weaken the integrity of the skin barrier and cause tingling and redness in some sensitive skin; for this reason, the applicant finally determined after a lot of research and experimental verification that the mass percentage of Schisandra chinensis extract in this application should be the above.

[0011] Preferably, the mass percentage of the trollius extract is 2-4%.

[0012] By adopting the above technical solution, when the content of nasturtium lotus extract is too low, the nasturtium lotus extract cannot effectively inhibit the release of pro-inflammatory factors, and it is difficult to effectively soften the stratum corneum and expand the cell gap to guide and improve the penetration and absorption of other components; when the content of nasturtium lotus extract is too high, excessive organic acids will be introduced, thereby causing damage to the skin, weakening the integrity of the skin barrier, and causing dryness, desquamation and even sensitive reactions; for this reason, the applicant finally determined after a lot of research and experimental verification that the mass percentage of the nasturtium lotus extract in this application should be above.

[0013] Preferably, the polyol is at least one of glycerol, butylene glycol, 1,3-propanediol, propylene glycol, and dipropylene glycol.

[0014] Preferably, the mass percentage of the polyol is 30%.

[0015] By adopting the above technical solution, glycerin, butylene glycol, 1,3-propanediol, propylene glycol, and dipropylene glycol are all small molecule moisturizers that can bind to water through hydrogen bonds to form a long-lasting moisturizing layer to relieve dry skin; at the same time, the above polyols have good compatibility with plant extracts, can avoid precipitation or stratification, and can maintain the uniformity of the formula as solvents.

[0016] When the amount of polyol added is too low, it is not enough to provide sufficient moisturizing effect and it is difficult to compensate for the skin dryness that may be caused by the extract ingredients; when the amount of polyol added is too high, the moisturizing and soothing effect of the composition is difficult to further enhance, and excessive polyol will increase the stickiness of the composition, affecting the user experience; for this reason, the applicant finally determined after a lot of research and experimental verification that the mass percentage of the polyol in this application is preferably the above.

[0017] Preferably, the soothing composition is composed of the following raw materials in the following mass percentages: 25% Sophora japonica bud extract, 15% Schisandra chinensis extract, 15% Astragalus membranaceus extract, 3% Coix seed extract, 3% Trollius chinensis extract, 30% polyol and 9% purified water.

[0018] By adopting the above technical solution and optimizing the ratio of each component, the effect of the composition in inhibiting the release of inflammatory factors and soothing the symptoms is enhanced, achieving synergistic effects in many aspects.

[0019] In a second aspect, the present application provides a method for preparing a composition having a soothing effect, comprising the following steps: S1. The sophora japonica buds, schisandra chinensis, roasted astragalus root, coix seed and trollius were washed, dried and crushed to obtain raw material powders; S2. The raw material powders were decocted with water and filtered to obtain a filter residue and a first filtrate; the filter residue was then decocted with water again and filtered to obtain a second filtrate; the first filtrate and the second filtrate were mixed and further purified to obtain an extract solution; the extract solution was vacuum dried and then crushed and sieved to obtain sophora japonica bud extract powder, schisandra chinensis extract powder, roasted astragalus root extract powder, coix seed extract powder and trollius extract powder respectively; S3. Sophora japonica bud extract powder, Schisandra chinensis extract powder, roasted Astragalus root extract powder, Coix seed extract powder and Nasturtium root extract powder were dissolved in water to obtain Sophora japonica bud extract, Schisandra chinensis extract, roasted Astragalus root extract, Coix seed extract and Nasturtium root extract, and then mixed according to the formula amount to obtain a plant composition solution; S4. Add the formulated amount of polyol to the plant composition solution, stir evenly to fully dissolve it, then add purified water, stir evenly, and filter to obtain the soothing composition.

[0020] By adopting the above technical solution, the raw materials can be crushed to fully break the cell walls and increase the specific surface area of the extract powder; During the secondary decoction process, the first decoction can release polar components such as water-soluble polysaccharides and flavonoid glycosides, while the second decoction can promote cell rupture through osmotic pressure difference and extract medium and low polar components such as bound phenolic acids and esters, thus achieving effective collection of various components in the raw material powder. The order of adding polyols followed by purified water utilizes the hydrogen bonding of the polyols to form a "molecular cage" that pre-encapsulates hydrophobic components such as flavonoids and alkaloids, achieving uniform distribution of the components in the composition and reducing precipitation; The preparation method is simple to operate and has high bioavailability of active ingredients. While ensuring efficient extraction of active ingredients, it achieves multi-component synergistic enhancement and system stability, effectively improving the composition's inhibition of inflammatory factor release and soothing effects.

[0021] In a third aspect, the present application provides a cosmetic product that adopts the following technical solution: A cosmetic comprises the above-mentioned composition with soothing effect, and the composition has excellent effects of inhibiting the release of inflammatory factors and soothing.

[0022] In summary, this application has the following beneficial effects: 1. This application forms a multi-target soothing mechanism by using Sophora japonica bud extract in combination with Schisandra chinensis extract, Astragalus membranaceus extract, Coix seed extract, and Nasturtium extract: Sophora japonica bud extract inhibits the release of inflammatory mediators and regulates immune response, Schisandra chinensis and Astragalus membranaceus extract enhance immunity and inhibit the release of inflammatory factors, Coix seed extract is anti-inflammatory and antioxidant, and Nasturtium extract is anti-inflammatory and promotes penetration and absorption, thereby jointly achieving the effect of inhibiting the release of inflammatory factors and soothing.

[0023] 2. This application achieves a synergistic effect by scientifically combining multiple plant extracts: the anti-inflammatory effect of Sophora japonica extract combined with the immunomodulatory effect of Schisandra chinensis extract can more comprehensively relieve skin inflammation; the anti-inflammatory and anti-allergic effects of Sophora japonica bud extract are enhanced by Schisandra chinensis and Astragalus membranaceus extracts, the moisturizing effect of Coix lachryma-jobi extract relieves dryness and itching of the skin, and the guiding effect of Nasturtium flower extract improves the penetration and absorption of other ingredients, thereby achieving better inhibition of inflammatory factor release and soothing effects; 3. All plant extracts in this application are derived from natural plants, are scientifically extracted and compounded, contain no chemical preservatives and artificial synthetic ingredients, and are gentle and non-irritating to sensitive skin. This combination of natural ingredients is not only safe and reliable, but also can reduce the occurrence of allergic reactions.

[0024] 4. By optimizing the extraction process and formula design, the composition of the present application has good stability in cosmetics. Its inhibition of inflammatory factor release and soothing effects are not only significant in the short term, but can also achieve long-term skin health improvements by repairing the skin barrier and regulating immune function. DETAILED DESCRIPTION

[0025] The raw materials in this application include the following parts: Polyol: A commercially available product of glycerol with a CAS number of 56-81-5 was used.

[0026] Preparation Example 1 A method for preparing a sophora japonica bud extract comprises the following steps: S1. Wash and dry the Sophora japonica buds and crush them to obtain Sophora japonica bud powder; S2. The sophora japonica bud powder was boiled with 12 times of water and filtered to obtain a sophora japonica bud residue and a first sophora japonica bud filtrate; the sophora japonica bud residue was boiled again with 10 times of water and filtered to obtain a second sophora japonica bud filtrate; the first sophora japonica bud filtrate and the second sophora japonica bud filtrate were mixed and then further purified to obtain a sophora japonica bud extract solution; the sophora japonica bud extract solution was vacuum dried and then crushed and sieved to obtain a sophora japonica bud extract powder; S3. Dissolve 5 g of Sophora japonica bud extract powder in 95 g of water to obtain a 5 wt% Sophora japonica bud extract.

[0027] Preparation Example 2 A method for preparing a Schisandra chinensis extract comprises the following steps: S1. The schisandra chinensis was washed, dried and crushed to obtain schisandra chinensis powder; S2. The schisandra powder was decocted with 12 times of water and filtered to obtain a schisandra residue and a first filtrate of schisandra; the schisandra residue was then decocted again with 10 times of water and filtered to obtain a second filtrate of schisandra; the first filtrate of schisandra and the second filtrate of schisandra were mixed and then further purified to obtain a schisandra extract solution; the schisandra extract solution was vacuum dried and crushed and sieved to obtain a schisandra extract powder; S3. Dissolve 5 g of Schisandra chinensis extract powder in 95 g of water to obtain a 5 wt% Schisandra chinensis extract solution.

[0028] Preparation Example 3 A method for preparing a roasted Astragalus root extract comprises the following steps: S1. Wash and dry the Astragalus and crush it to obtain Astragalus powder; S2. The Astragalus powder was boiled with 15 times of water and filtered to obtain a residue and a first filtrate of Astragalus; the residue was then boiled again with 10 times of water and filtered to obtain a second filtrate of Astragalus; the first filtrate of Astragalus and the second filtrate of Astragalus were mixed and then further purified to obtain an Astragalus extract solution; the Astragalus extract solution was vacuum dried and crushed and sieved to obtain an Astragalus extract powder; S3. Dissolve 5 g of the Astragalus membranaceus extract powder in 95 g of water to obtain a 5 wt% Astragalus membranaceus extract solution.

[0029] Preparation Example 4 A method for preparing a coix seed extract comprises the following steps: S1. Wash, dry and crush the coix seed to obtain coix seed powder; S2. The coix seed powder was boiled with 12 times of water and filtered to obtain a coix seed residue and a first filtrate of coix seed; the coix seed residue was then boiled with 10 times of water again and filtered to obtain a second filtrate of coix seed; the first filtrate of coix seed and the second filtrate of coix seed were mixed and then further purified to obtain a coix seed extract solution; the coix seed extract solution was vacuum dried and crushed and sieved to obtain a coix seed extract powder; S3. Dissolve 5 g of coix seed extract powder in 95 g of water to obtain a 5 wt% coix seed extract solution.

[0030] Preparation Example 5 A method for preparing a trollius extract comprises the following steps: S1. Wash, dry and crush the nasturtium to obtain nasturtium powder; S2. The truncatum powder was boiled with 12 times of water and filtered to obtain a truncatum residue and a first filtrate of truncatum; the truncatum residue was then boiled again with 10 times of water and filtered to obtain a second filtrate of truncatum; the first filtrate and the second filtrate of truncatum were mixed and then further purified to obtain a truncatum extract solution; the truncatum extract solution was vacuum dried and crushed and sieved to obtain a truncatum extract powder; S3. Dissolve 5 g of Trollius extract powder in 95 g of water to obtain a 5 wt% Trollius extract solution.

[0031] The present application is further described in detail below with reference to the following examples and comparative examples.

[0032] Example 1 A method for preparing a composition having a soothing effect comprises the following steps: S1. 25g of the Sophora japonica bud extract of Preparation Example 1, 15g of the Schisandra chinensis extract of Preparation Example 2, 15g of the roasted Astragalus extract of Preparation Example 3, 3g of the Coix seed extract of Preparation Example 4 and 3g of the Nasturtium extract of Preparation Example 5 were mixed to obtain a plant composition solution; S2. Add 30 g of glycerol to the plant composition solution, stir evenly to fully dissolve it, then add purified water to make up to 100 g, stir evenly and filter to obtain the soothing composition.

[0033] Example 2 A method for preparing a composition having a soothing effect comprises the following steps: S1. 10 g of the Sophora japonica bud extract of Preparation Example 1, 20 g of the Schisandra chinensis extract of Preparation Example 2, 10 g of the roasted Astragalus membranaceus extract of Preparation Example 3, 1 g of the Coix lachryma-jobi extract of Preparation Example 4, and 1 g of the Trollius chinensis extract of Preparation Example 5 were mixed to obtain a plant composition solution; S2. Add 20 g of glycerol to the plant composition solution, stir evenly to fully dissolve it, then add purified water to make up to 100 g, stir evenly and filter to obtain the soothing composition.

[0034] Example 3 A method for preparing a composition having a soothing effect comprises the following steps: S1. 40 g of the Sophora japonica bud extract of Preparation Example 1, 10 g of the Schisandra chinensis extract of Preparation Example 2, 20 g of the roasted Astragalus extract of Preparation Example 3, 5 g of the Coix seed extract of Preparation Example 4 and 5 g of the Nasturtium extract of Preparation Example 5 were mixed to obtain a plant composition solution; S2. Add 40 g of glycerol to the plant composition solution, stir evenly to fully dissolve it, stir evenly and filter to obtain the soothing composition.

[0035] Example 4 A method for preparing a composition having a soothing effect comprises the following steps: S1. 25g of the Sophora japonica bud extract of Preparation Example 1, 12g of the Schisandra chinensis extract of Preparation Example 2, 15g of the roasted Astragalus extract of Preparation Example 3, 3g of the Coix seed extract of Preparation Example 4 and 3g of the Nasturtium extract of Preparation Example 5 were mixed to obtain a plant composition solution; S2. Add 30 g of glycerol to the plant composition solution, stir evenly to fully dissolve it, then add purified water to make up to 100 g, stir evenly and filter to obtain the soothing composition.

[0036] Example 5 A method for preparing a composition having a soothing effect comprises the following steps: S1. 25g of the Sophora japonica bud extract of Preparation Example 1, 17g of the Schisandra chinensis extract of Preparation Example 2, 15g of the roasted Astragalus extract of Preparation Example 3, 3g of the Coix seed extract of Preparation Example 4 and 3g of the Nasturtium extract of Preparation Example 5 were mixed to obtain a plant composition solution; S2. Add 30 g of glycerol to the plant composition solution, stir evenly to fully dissolve it, then add purified water to make up to 100 g, stir evenly and filter to obtain the soothing composition.

[0037] Example 6 A method for preparing a composition having a soothing effect comprises the following steps: S1. 25g of the Sophora japonica bud extract of Preparation Example 1, 15g of the Schisandra chinensis extract of Preparation Example 2, 15g of the roasted Astragalus extract of Preparation Example 3, 3g of the Coix seed extract of Preparation Example 4 and 2g of the Nasturtium extract of Preparation Example 5 were mixed to obtain a plant composition solution; S2. Add 30 g of glycerol to the plant composition solution, stir evenly to fully dissolve it, then add purified water to make up to 100 g, stir evenly and filter to obtain the soothing composition.

[0038] Example 7 A method for preparing a composition having a soothing effect comprises the following steps: S1. 25g of the Sophora japonica bud extract of Preparation Example 1, 15g of the Schisandra chinensis extract of Preparation Example 2, 15g of the roasted Astragalus extract of Preparation Example 3, 3g of the Coix seed extract of Preparation Example 4 and 4g of the Nasturtium extract of Preparation Example 5 were mixed to obtain a plant composition solution; S2. Add 30 g of glycerol to the plant composition solution, stir evenly to fully dissolve it, then add purified water to make up to 100 g, stir evenly and filter to obtain the soothing composition.

[0039] Example 8 A method for preparing a composition having a soothing effect comprises the following steps: S1. 25g of the Sophora japonica bud extract of Preparation Example 1, 15g of the Schisandra chinensis extract of Preparation Example 2, 15g of the roasted Astragalus extract of Preparation Example 3, 3g of the Coix seed extract of Preparation Example 4 and 3g of the Nasturtium extract of Preparation Example 5 were mixed to obtain a plant composition solution; S2. Add 20 g of glycerol to the plant composition solution, stir evenly to fully dissolve it, then add purified water to make up to 100 g, stir evenly and filter to obtain the soothing composition.

[0040] Example 9 A method for preparing a composition having a soothing effect comprises the following steps: S1. 25g of the Sophora japonica bud extract of Preparation Example 1, 15g of the Schisandra chinensis extract of Preparation Example 2, 15g of the roasted Astragalus extract of Preparation Example 3, 3g of the Coix seed extract of Preparation Example 4 and 3g of the Nasturtium extract of Preparation Example 5 were mixed to obtain a plant composition solution; S2. Add 39 g of glycerol to the plant composition solution, stir evenly to fully dissolve it, stir evenly and filter to obtain the soothing composition.

[0041] Comparative Example 1 A method for preparing a composition having a soothing effect comprises the following steps: S1. 15g of the Schisandra chinensis extract of Preparation Example 2, 15g of the roasted Astragalus extract of Preparation Example 3, 3g of the Coix seed extract of Preparation Example 4 and 3g of the Trollius extract of Preparation Example 5 were mixed to obtain a plant composition solution; S2. Add 30 g of glycerol to the plant composition solution, stir evenly to fully dissolve it, then add purified water to make up to 100 g, stir evenly and filter to obtain the soothing composition.

[0042] Comparative Example 2 A method for preparing a composition having a soothing effect comprises the following steps: S1. 25 g of the Sophora japonica bud extract of Preparation Example 1, 15 g of the roasted Astragalus extract of Preparation Example 3, 3 g of the Coix seed extract of Preparation Example 4 and 3 g of the Nasturtium extract of Preparation Example 5 were mixed to obtain a plant composition solution; S2. Add 30 g of glycerol to the plant composition solution, stir evenly to fully dissolve it, then add purified water to make up to 100 g, stir evenly and filter to obtain the soothing composition.

[0043] Comparative Example 3 A method for preparing a composition having a soothing effect comprises the following steps: S1. 25 g of the Sophora japonica bud extract of Preparation Example 1, 15 g of the Schisandra chinensis extract of Preparation Example 2, 3 g of the Coix lachryma-jobi extract of Preparation Example 4, and 3 g of the Nasturtium extract of Preparation Example 5 were mixed to obtain a plant composition solution; S2. Add 30 g of glycerol to the plant composition solution, stir evenly to fully dissolve it, then add purified water to make up to 100 g, stir evenly and filter to obtain the soothing composition.

[0044] Comparative Example 4 A method for preparing a composition having a soothing effect comprises the following steps: S1. 25g of the Sophora japonica bud extract of Preparation Example 1, 15g of the Schisandra chinensis extract of Preparation Example 2, 15g of the roasted Astragalus extract of Preparation Example 3 and 3g of the Trollius extract of Preparation Example 5 were mixed to obtain a plant composition solution; S2. Add 30 g of glycerol to the plant composition solution, stir evenly to fully dissolve it, then add purified water to make up to 100 g, stir evenly and filter to obtain the soothing composition.

[0045] Comparative Example 5 A method for preparing a composition having a soothing effect comprises the following steps: S1. 25g of the Sophora japonica bud extract of Preparation Example 1, 15g of the Schisandra chinensis extract of Preparation Example 2, 15g of the roasted Astragalus membranaceus extract of Preparation Example 3 and 3g of the Coix seed extract of Preparation Example 4 were mixed to obtain a plant composition solution; S2. Add 30 g of glycerol to the plant composition solution, stir evenly to fully dissolve it, then add purified water to make up to 100 g, stir evenly and filter to obtain the soothing composition.

[0046] Performance testing The compositions of Examples 1-9 and Comparative Examples 1-5 were subjected to the following performance tests. The test results are shown in Table 1: (1) Testing of inflammatory factor IL-6 Bacterial lipopolysaccharide (LPS) binds to antigen-recognition receptors on the surface of macrophages, inducing the secretion of multiple cytokines, including IL-6. IL-6 is a key inflammatory factor, activating three signaling pathways: caspase, JNK, and the transcription factor NF-κB. This activates IL-6, which promotes its cytotoxic, antiviral, immunomodulatory, and apoptotic functions.

[0047] The IL-6 content was determined using an enzyme-linked immunosorbent assay (ELISA). The specific principle is: IL-6 specifically binds to the IL-6 antibody coated on the ELISA plate, and then binds to the anti-IL-6 antibody labeled with a substrate. After the substrate is catalyzed by the enzyme, a colored product is generated. The IL-6 content is positively correlated with the color depth of the colored product.

[0048] The test steps are as follows: (A) Set up two test substance groups as follows: Negative control group: basal medium containing LPS; Sample group: the composition samples with soothing effects of Examples 1-9 and Comparative Examples 1-5 + a basic culture medium containing LPS.

[0049] (B) Cell culture: Cryopreserved cell cultures were inoculated into conventional culture medium at an appropriate density for use in experiments.

[0050] (C) Cell viability assay: RAW264.7 cells were plated in 96-well plates. After 24 hours, the culture medium was aspirated and the basal culture medium containing the test sample was added. After 24 hours, the OD490 value was measured by MTT assay, and the effect of the test sample on the viability of RAW264.7 cells was calculated: IL-6 inhibition rate = (1-average IL-6 content of the two sample groups / average IL-6 content of the negative control) × 100%.

[0051] (2) Soothing effect test Sodium lauryl sulfate (SLS) can trigger an irritant response in the body. This irritant enters zebrafish, inducing an inflammatory response. Neutrophils initiate an immune response, migrating and concentrating in the skin epidermis. The soothing effect of the sample was tested by measuring changes in skin neutrophil counts before and after treatment with a transgenic neutrophil green fluorescent strain (MPX).

[0052] The soothing effect test includes the following steps: (A) Zebrafish were randomly selected and placed in a 6-well plate, with 15 zebrafish per well.

[0053] (B) A zebrafish skin inflammation model was established by aqueous administration of SLS.

[0054] (C) Water-soluble administration of the soothing composition samples of Examples 1-9 and Comparative Examples 1-5 as a sample group, and a normal control group and a model control group were also set up, with a capacity of 3 g per well; Normal control group: zebrafish were not exposed to SLS or any test samples and were only exposed to normal breeding environment; Model control group: zebrafish were exposed to SLS via water solution to induce skin inflammatory response (neutrophils migrated to the epidermis and aggregated), but were not given intervention with the samples of Examples 1-9 and Comparative Examples 1-5.

[0055] (D) Incubate at 28.5°C in the dark for 18 h.

[0056] (E) Ten zebrafish were randomly selected from each experimental group and photographed under a fluorescence microscope. Advanced image processing software was used to analyze and collect data. The number of neutrophils in the zebrafish skin was analyzed, and the soothing efficacy value of the sample was calculated according to the formula to determine whether it had soothing efficacy: Soothing efficacy = (model control group - sample group) / (model control group - normal control group) × 100%.

[0057] Table 1: Raw materials and performance test table of the compositions with soothing effect of Examples 1-9 and Comparative Examples 1-5 Referring to Table 1, by comparing Example 1 with Comparative Examples 1-5, it can be seen that the inhibition rate and soothing efficacy of the composition of Example 1 are higher than those of Comparative Examples 1-5, indicating that the combination of Sophora japonica bud extract, Schisandra chinensis extract, Astragalus membranaceus extract, Coix seed extract, and Trollius chinensis extract can form a multi-target mechanism for inhibiting the release of inflammatory factors, and cooperate with each other in different mechanisms to jointly achieve the effects of inhibiting the release of inflammatory factors and soothing.

[0058] By comparing Example 1 and Examples 4-5, it can be seen that when the content of Schisandra chinensis extract is too low or too high, the IL-6 inhibition rate and soothing effect of the composition will decrease. This is because when the content of Schisandra chinensis extract is too low, its enhancing effect on the soothing effect of Astragalus membranaceus extract is weak; when the content of Schisandra chinensis extract is too high, too much organic acid will be introduced into the composition, weakening the integrity of the skin barrier.

[0059] By comparing Example 1 with Examples 6-7, it can be seen that a content of too low or too high nasturtium extract will lead to a decrease in the IL-6 inhibition rate and soothing effect of the composition. This is because a content of too low nasturtium extract cannot effectively inhibit the release of pro-inflammatory factors, and it is difficult to effectively soften the stratum corneum and expand the intercellular gaps to guide and improve the penetration and absorption of other ingredients; a content of too high nasturtium extract will introduce excessive organic acids, thereby damaging the skin, weakening the integrity of the skin barrier, and causing dryness, desquamation, and even sensitive reactions.

[0060] By comparing Example 1 and Examples 8-9, it can be seen that as the glycerol content continues to increase, the IL-6 inhibition rate and soothing effect of the composition continue to increase and then tend to be flat. This is because glycerol, as a small molecule moisturizer, can bind to water through hydrogen bonds to form a long-lasting moisturizing layer, thereby alleviating dry skin; when the glycerol content exceeds a certain range, the moisturizing effect of glycerol is difficult to improve, so that the IL-6 inhibition rate and soothing effect of the composition are no longer further improved.

[0061] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed, but as long as they are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. A composition having a soothing effect, characterized in that The invention is composed of the following raw materials in percentage by mass: 10-25% of Sophora japonica bud extract, 10-20% of Schisandra chinensis chinensis extract, 10-20% of Astragalus membranaceus extract, 1-5% of Coix lachryma-jobi seed extract, 1-5% of Trollius chinensis extract, 20-40% of polyol, and the balance of purified water; The mass concentrations of the Sophora japonica bud extract, Schisandra chinensis chinensis extract, Astragalus membranaceus extract, Coix lachryma-jobi seed extract, and Trollius truncatum extract are all 4-6 wt %.

2. The soothing composition according to claim 1, characterized in that: The mass percentage of the Schisandra chinensis extract is 12-17%.

3. The soothing composition according to claim 1, characterized in that: The mass percentage of the trollius extract is 2-4%.

4. The soothing composition according to claim 1, characterized in that: The polyol is at least one of glycerol, butylene glycol, 1,3-propanediol, propylene glycol, and dipropylene glycol.

5. The soothing composition according to claim 4, characterized in that: The mass percentage of the polyol is 30%.

6. The soothing composition according to claim 1, characterized in that The preparation method is composed of the following raw materials in the following mass percentages: 25% of Sophora japonica bud extract, 15% of Schisandra chinensis chinensis extract, 15% of roasted Astragalus membranaceus extract, 3% of Coix lachryma-jobi seed extract, 3% of Trollius chinensis extract, 30% of polyol and 9% of purified water.

7. A method for preparing the composition having a soothing effect according to any one of claims 1 to 6, characterized in that: The following steps are involved: S1. The Sophora japonica buds, Schisandra chinensis, roasted Astragalus membranaceus, Coix lachryma-jobi and Nasturtium were washed, dried and crushed to obtain raw material powder; S2. The raw material powders were decocted with water and filtered to obtain a filter residue and a first filtrate; the filter residue was then decocted with water again and filtered to obtain a second filtrate; the first filtrate and the second filtrate were mixed and further purified to obtain an extract solution; the extract solution was vacuum dried and crushed and sieved to obtain Sophora japonica bud extract powder, Schisandra chinensis extract powder, Astragalus membranaceus extract powder, Coix seed extract powder and Nasturtium extract powder; S3. Sophora japonica bud extract powder, Schisandra chinensis extract powder, roasted Astragalus root extract powder, Coix seed extract powder and Nasturtium root extract powder were dissolved in water to obtain Sophora japonica bud extract, Schisandra chinensis extract, roasted Astragalus root extract, Coix seed extract and Nasturtium root extract, and then mixed according to the formula amount to obtain a plant composition solution; S4. Add the formulated amount of polyol to the plant composition solution, stir evenly to fully dissolve it, then add purified water, stir evenly, and filter to obtain the composition having a soothing effect.

8. A cosmetic, characterized in that: The invention comprises a composition having a soothing effect as described in any one of claims 1 to 6.

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