Traditional Chinese medicine electuary for preventing hair loss and growing hair as well as preparation method and application thereof

Abstract

The invention provides a traditional Chinese medicine electuary for preventing hair loss and growing hair as well as a preparation method and application thereof, the traditional Chinese medicine electuary is prepared from the following components in parts by weight: 800 to 1000 parts of nano rhodiola rosea extract, 100 to 200 parts of fructus ligustri lucidi, 20 to 30 parts of astragaloside, 20 to 30 parts of rhizoma chuanxiong, 0.03 to 0.05 part of selenomethionine, 50 to 100 parts of mannose oligomer (MOS) and 60 to 90 parts of chitosan / sodium alginate conjugate. And 30-60 parts of an animal pancreas extract. A certain amount of micro-capsule components, glossy privet fruits, astragaloside, ligusticum wallichii, selenomethionine and animal pancreas extracts are uniformly mixed to obtain the traditional Chinese medicine electuary for preventing alopecia and promoting hair growth, all the medicines supplement one another, and the traditional Chinese medicine electuary has the effects of dredging collaterals and promoting hair growth, takes effect quickly and can promote hair growth more quickly. Pharmacodynamic experiments show that the traditional Chinese medicine electuary has remarkable effects of preventing alopecia and promoting hair growth, has no toxic or side effect, and has a good clinical application prospect.

Description

Technical Field

[0001] The invention specifically relates to a hair loss prevention and hair growth Chinese medicine granule and a preparation method and application thereof, belonging to the field of Chinese medicine. Background Art

[0002] Today's high-pressure, fast-paced lifestyle creates significant stress, leading to premature hair loss and shortened hair follicle growth. In healthy individuals, normally shed hair is in the catagen and telogen phases. Because the hair entering the catagen phase and the newly entering anagen phase maintain a constant dynamic balance, a normal hair count is maintained. An increasing number of people are experiencing abnormal or excessive hair loss due to various factors, including stress, endocrine abnormalities, malnutrition, and metabolic abnormalities. Although hair loss may not cause conscious symptoms, it can have a significant psychological impact on those experiencing it, often negatively impacting their daily lives. With rising living standards, people are placing increasing emphasis on hair loss treatment, leading to a growing market demand for products to prevent and treat hair loss.

[0003] At present, in order to prevent hair loss and promote hair growth, existing technologies have developed various hair growth formulas and hair growth products for treating hair and caring for the scalp. However, these products usually have defects such as high production costs, poor ease of use, and unsatisfactory results. There is also no economical and convenient Chinese medicine for internal use in the current market that has good hair growth and anti-hair loss effects.

[0004] Therefore, providing a drug with significant hair growth effect and no obvious adverse reactions has become an urgent problem to be solved. Summary of the Invention

[0005] In order to overcome the deficiencies of the prior art, the present invention provides a hair loss prevention and hair growth promoting Chinese medicine granule and a preparation method and application thereof.

[0006] The technical solution adopted by the present invention to solve the technical problem is:

[0007] On the one hand, the present invention provides a traditional Chinese medicine granule for preventing hair loss and promoting hair growth, which is composed of the following components in parts by weight: 800-1000 parts of nano-Rhodiola rosea extract, 100-200 parts of Ligustrum lucidum fruit, 20-30 parts of astragaloside IV, 20-30 parts of Chuanxiong rhizome, 0.03-0.05 parts of selenomethionine, 50-100 parts of oligomannose (MOS), 60-90 parts of chitosan / sodium alginate conjugate, and 30-60 parts of animal pancreatic extract.

[0008] Furthermore, the weight ratio of nano-Rhodiola rosea extract to oligomannose (MOS) is (10-12):1.

[0009] In one aspect, the present invention further provides a method for preparing a hair loss prevention and hair growth Chinese medicine granule. Specifically, the Chinese medicine granule is prepared by the following steps:

[0010] (1) Preparation of nano-Rhodiola rosea extract: placing Rhodiola rosea in distilled water, adding cellulase, adjusting the pH of the system to 4.5-5.5, heating to 45°C-65°C for reaction for 1-2 hours, cooling the reaction product to a freeze-drying temperature at a cooling rate of 80-100°C / min, freeze-drying at -60--80°C for 12-36 hours, ultrafinely grinding to 300-500 nm in a liquid nitrogen environment at -60--80°C, and heating from the grinding temperature to room temperature, wherein the specific heating process from the grinding temperature to room temperature is: heating the grinding and drying temperature to -40°C, keeping warm for 30 minutes, -40°C to -10°C, keeping warm for 50 minutes, and -10°C to room temperature, to obtain the nano-Rhodiola rosea extract;

[0011] (2) Preparing an animal pancreatic extract: obtaining a fresh animal pancreas, adding a protease inhibitor to the pancreas, crushing the pancreas into a paste, then adding a protein extract, stirring and centrifuging at a low temperature of 0-4°C, collecting the supernatant, and filtering the supernatant with a microfiltration membrane to remove impurities, thereby obtaining the animal pancreatic extract;

[0012] (3) taking a certain amount of nano-Rhodiola rosea extract and oligomannose (MOS) and mixing them evenly, adding the mixture to a sodium alginate aqueous solution to form a suspension, electrostatically spraying the suspension, dropping it into a mixed solution formed by chitosan and calcium chloride, solidifying it into microspheres, and separating the microspheres to obtain a sustained-release microcapsule component;

[0013] (4) A certain amount of sustained-release microcapsule components, Ligustrum lucidum fruit, astragaloside IV, Chuanxiong rhizome, selenomethionine, and animal pancreatic extract are mixed evenly to obtain a Chinese medicine granule for preventing hair loss and promoting hair growth.

[0014] Furthermore, the microfiltration membrane is a 0.45 micron microfiltration membrane.

[0015] Furthermore, the animal pancreas is a cow or rabbit pancreas.

[0016] Furthermore, the protease inhibitor is phenylmethylsulfonyl fluoride (PMSF), and the protein extract is phosphate buffer solution or physiological saline.

[0017] The present invention finds that by adopting an extremely rapid cooling method to reduce the reaction product to the freeze-drying temperature and perform freeze-drying, while controlling the process of raising the pulverization temperature to room temperature, and adopting a step-by-step heating method to slowly raise the temperature of the extract to room temperature, the heating and cooling process of the extract is controlled, thereby better retaining the pharmaceutical activity of the Rhodiola rosea extract, being more conducive to regulating the autophagy of hair follicle stem cells, prolonging the growth period of hair follicles, and better retaining the active ingredients of antioxidant enzymes, removing reactive oxygen species in hair follicle cells, and improving the mitochondrial function of hair follicle cells.

[0018] The oligomannosaccharides (MOS) used in this invention can indirectly reduce systemic oxidative stress by promoting the proliferation of intestinal probiotics and increasing the production of short-chain fatty acids. At the same time, its degradation products can inhibit the colonization of pathogenic bacteria such as Malassezia and suppress the production of reactive oxygen species by harmful bacteria. Nano-rhodiola rosea extract directly enhances the activity of antioxidant enzymes, reducing the damage caused by oxidative stress to hair follicle stem cells. The specific weight ratio of oligomannosaccharides (MOS) and nano-rhodiola rosea extract synergizes with each other. Nano-rhodiola rosea extract enhances the cells' endogenous antioxidant capacity, while oligomannosaccharides (MOS) reduce exogenous stressors, forming an "internal and external double barrier" to protect the hair follicle microenvironment.

[0019] On the one hand, the present invention also provides a use of a traditional Chinese medicine granule for preventing hair loss and promoting hair growth, specifically, the use of the traditional Chinese medicine granule for preventing hair loss and promoting hair growth in promoting hair growth and preventing hair loss.

[0020] Beneficial effects:

[0021] (1) The nano-Rhodiola rosea extract and oligomannose (MOS) used in the present invention both have good biocompatibility. Encapsulating them in chitosan / sodium alginate microcapsules results in a Chinese medicine granule with an encapsulation rate of ≥90%, an intestinal release rate of ≤3%, and a scalp-targeted release of ≥80%, achieving co-delivery and improving the scalp targeting of the Chinese medicine granule. The present invention uses a protease inhibitor to obtain an animal pancreatic extract, which is applied to a Chinese medicine granule for preventing hair loss and promoting hair growth, resulting in good anti-hair loss and hair growth effects.

[0022] (2) The present invention provides a Chinese herbal granule for preventing hair loss and promoting hair growth, as well as its preparation method and application. The various herbs in the Chinese herbal granule complement each other, have the effects of invigorating qi and nourishing blood, promoting blood circulation and promoting hair growth, and have a rapid onset of action, which can promote hair growth more quickly. Pharmacological efficacy experiments have shown that the Chinese herbal granule of the present invention has significant effects in preventing hair loss and promoting hair growth, has no toxic side effects, and has good clinical application prospects. DETAILED DESCRIPTION

[0023] The present invention is further illustrated below with reference to specific examples. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. After reading the present invention, modifications of various equivalent forms of the present invention made by those skilled in the art all fall within the scope defined by the claims attached to this application.

[0024] Unless otherwise stated, the raw materials and equipment used in the embodiments of the present invention are raw materials and equipment of conventional sources in the art. The endpoints and any values of the ranges disclosed herein are not limited to the precise ranges or values, and these ranges or values should be understood to include values close to these ranges or values. For numerical ranges, the endpoint values of each range, the endpoint values of each range and a separate point value, and the separate point values can be combined with each other to obtain one or more new numerical ranges, and these numerical ranges should be considered to be specifically disclosed in this article.

[0025] Example 1:

[0026] A traditional Chinese medicine granule for preventing hair loss and promoting hair growth is composed of the following components in parts by weight: 840 parts of nano-rhodiola rosea extract, 120 parts of glossy privet fruit, 20 parts of astragaloside IV, 20 parts of ligusticum chuanxiong, 0.03 parts of selenomethionine, 70 parts of oligomannose (MOS), 65 parts of chitosan / sodium alginate conjugate, and 35 parts of animal pancreas extract.

[0027] The weight ratio of nano-Rhodiola rosea extract to oligomannosaccharides (MOS) is 12:1.

[0028] The preparation method of the said anti-hair loss and hair growth Chinese medicine granules is specifically prepared by the following steps:

[0029] (1) Preparation of nano-Rhodiola rosea extract: placing Rhodiola rosea in distilled water, adding cellulase, adjusting the pH of the system to 4.5, heating to 45°C for reaction for 1 hour, cooling the reaction product to a freeze-drying temperature at a cooling rate of 80°C / min, freeze-drying at -60°C for 36 hours, ultrafinely grinding to 350nm in a liquid nitrogen environment at -60°C, and heating from the grinding temperature to room temperature, wherein the specific heating process from the grinding temperature to room temperature is: heating the grinding and drying temperature to -40°C, keeping warm for 30 minutes, -40°C to -10°C, keeping warm for 50 minutes, and -10°C to room temperature, to obtain the nano-Rhodiola rosea extract;

[0030] (2) Preparing an animal pancreatic extract: obtaining a fresh animal pancreas, adding a protease inhibitor to the pancreas, crushing the pancreas into a paste, and then adding a protein extract. The mixture is stirred and centrifuged at 0°C, and the supernatant is collected. The supernatant is filtered through a microfiltration membrane to remove impurities, thereby obtaining the animal pancreatic extract.

[0031] (3) taking a certain amount of nano-Rhodiola rosea extract and oligomannose (MOS) and mixing them evenly, adding the mixture to a sodium alginate aqueous solution to form a suspension, electrostatically spraying the suspension, dropping it into a mixed solution formed by chitosan and calcium chloride, solidifying it into microspheres, and separating the microspheres to obtain a sustained-release microcapsule component;

[0032] (4) A certain amount of sustained-release microcapsule components, Ligustrum lucidum fruit, astragaloside IV, Chuanxiong rhizome, selenomethionine and animal pancreatic extract are mixed evenly to obtain a Chinese medicine granule for preventing hair loss and promoting hair growth.

[0033] The microfiltration membrane is a 0.45 micron filter membrane.

[0034] The animal pancreas used was rabbit pancreas.

[0035] The protease inhibitor was phenylmethylsulfonyl fluoride (PMSF), and the protein extract was phosphate buffered saline.

[0036] Example 2:

[0037] A traditional Chinese medicine granule for preventing hair loss and promoting hair growth is composed of the following components in parts by weight: 880 parts of nano-rhodiola rosea extract, 150 parts of glossy privet fruit, 25 parts of astragaloside IV, 25 parts of ligusticum chuanxiong, 0.04 parts of selenomethionine, 80 parts of oligomannose (MOS), 75 parts of chitosan / sodium alginate conjugate, and 45 parts of animal pancreas extract.

[0038] The weight ratio of nano-Rhodiola rosea extract to oligomannose (MOS) is 11:1.

[0039] The preparation method of the said anti-hair loss and hair growth Chinese medicine granules is specifically prepared by the following steps:

[0040] (1) Preparation of nano-Rhodiola rosea extract: Rhodiola rosea was placed in distilled water, cellulase was added, the pH of the system was adjusted to 5, and the reaction was heated to 55°C for 1.5 hours. The reaction product was cooled to a freeze-drying temperature at a cooling rate of 90°C / min, freeze-dried at -70°C for 36 hours, ultrafinely ground to 350nm in a liquid nitrogen environment at -70°C, and heated from the grinding temperature to room temperature. The specific heating process from the grinding temperature to room temperature is as follows: the grinding and drying temperature was raised to -40°C, kept warm for 30 minutes, -40°C to -10°C, kept warm for 50 minutes, and -10°C to room temperature to obtain the nano-Rhodiola rosea extract;

[0041] (2) Preparing an animal pancreatic extract: obtaining a fresh animal pancreas, adding a protease inhibitor to the pancreas, crushing the pancreas into a paste, and then adding a protein extract. The mixture is stirred and centrifuged at a low temperature of 2°C, and the supernatant is collected. The supernatant is filtered through a microfiltration membrane to remove impurities, thereby obtaining the animal pancreatic extract.

[0042] (3) taking a certain amount of nano-Rhodiola rosea extract and oligomannose (MOS) and mixing them evenly, adding the mixture to a sodium alginate aqueous solution to form a suspension, electrostatically spraying the suspension, dropping it into a mixed solution formed by chitosan and calcium chloride, solidifying it into microspheres, and separating the microspheres to obtain a sustained-release microcapsule component;

[0043] (4) A certain amount of sustained-release microcapsule components, Ligustrum lucidum fruit, astragaloside IV, Chuanxiong rhizome, selenomethionine and animal pancreatic extract are mixed evenly to obtain a Chinese medicine granule for preventing hair loss and promoting hair growth.

[0044] The microfiltration membrane is a 0.45 micron filter membrane.

[0045] The animal pancreas used was rabbit pancreas.

[0046] The protease inhibitor was phenylmethylsulfonyl fluoride (PMSF), and the protein extract was phosphate buffered saline.

[0047] Example 3:

[0048] A traditional Chinese medicine granule for preventing hair loss and promoting hair growth is composed of the following components in parts by weight: 900 parts of nano-rhodiola rosea extract, 180 parts of glossy privet fruit, 30 parts of astragaloside IV, 30 parts of ligusticum chuanxiong, 0.05 parts of selenomethionine, 90 parts of oligomannose (MOS), 85 parts of chitosan / sodium alginate conjugate, and 55 parts of animal pancreas extract.

[0049] The weight ratio of nano-Rhodiola rosea extract to oligomannosaccharides (MOS) is 10:1.

[0050] The preparation method of the said anti-hair loss and hair growth Chinese medicine granules is specifically prepared by the following steps:

[0051] (1) Preparation of nano-Rhodiola rosea extract: placing Rhodiola rosea in distilled water, adding cellulase, adjusting the pH of the system to 5.5, heating to 65°C for reaction for 2 hours, cooling the reaction product to a freeze-drying temperature at a cooling rate of 100°C / min, freeze-drying at -80°C for 36 hours, ultrafinely grinding to 350nm in a liquid nitrogen environment at -80°C, and heating from the grinding temperature to room temperature, wherein the specific heating process from the grinding temperature to room temperature is: heating the grinding and drying temperature to -40°C, keeping warm for 30 minutes, -40°C to -10°C, keeping warm for 50 minutes, and -10°C to room temperature, to obtain the nano-Rhodiola rosea extract;

[0052] (2) Preparing an animal pancreatic extract: obtaining a fresh animal pancreas, adding a protease inhibitor to the pancreas, crushing the pancreas into a paste, and then adding a protein extract. The mixture is stirred and centrifuged at a low temperature of 4°C, and the supernatant is collected. The supernatant is filtered through a microfiltration membrane to remove impurities, thereby obtaining the animal pancreatic extract.

[0053] (3) taking a certain amount of nano-Rhodiola rosea extract and oligomannose (MOS) and mixing them evenly, adding the mixture to a sodium alginate aqueous solution to form a suspension, electrostatically spraying the suspension, dropping it into a mixed solution formed by chitosan and calcium chloride, solidifying it into microspheres, and separating the microspheres to obtain a sustained-release microcapsule component;

[0054] (4) A certain amount of sustained-release microcapsule components, Ligustrum lucidum fruit, astragaloside IV, Chuanxiong rhizome, selenomethionine and animal pancreatic extract are mixed evenly to obtain a Chinese medicine granule for preventing hair loss and promoting hair growth.

[0055] The microfiltration membrane is a 0.45 micron filter membrane.

[0056] The animal pancreas used was rabbit pancreas.

[0057] The protease inhibitor was phenylmethylsulfonyl fluoride (PMSF), and the protein extract was phosphate buffered saline.

[0058] Comparative Example 1: A Chinese herbal granule for preventing hair loss and promoting hair growth, which is different from Example 3 in that no animal pancreatic extract is added.

[0059] Comparative Example 2: A traditional Chinese medicine granule for preventing hair loss and promoting hair growth, comprising the following components in parts by weight: 960 parts of nano-Rhodiola rosea extract, 150 parts of Ligustrum lucidum fruit, 25 parts of astragaloside IV, 25 parts of Chuanxiong rhizome, 0.04 parts of selenomethionine, 75 parts of chitosan / sodium alginate conjugate, and 45 parts of animal pancreatic extract. The difference from Example 2 is that oligomannosaccharides (MOS) are not added, and the weight of nano-Rhodiola rosea extract is 960 parts.

[0060] Comparative Example 3: A Chinese herbal granule for preventing hair loss and promoting hair growth, which differs from Example 2 in that step (1) is different. Step (1) of Comparative Example 3 is to prepare a Rhodiola rosea extract: Rhodiola rosea is placed in distilled water, cellulase is added, the pH of the system is adjusted to 5, the mixture is heated to 55°C for reaction for 1.5 hours, and the reaction product is dried and crushed to obtain a Rhodiola rosea extract.

[0061] Comparative Example 4: A Chinese herbal granule for preventing hair loss and promoting hair growth, which is different from Example 2 in that: step (3) is not included, and the nano-rhodiola rosea extract and oligomannosaccharides (MOS) are not encapsulated in microcapsules. In step (4), a certain amount of nano-rhodiola rosea extract, Ligustrum lucidum fruit, astragaloside IV, Chuanxiong rhizome, selenomethionine, oligomannosaccharides (MOS) and animal pancreatic extract are directly mixed uniformly to obtain a Chinese herbal granule for preventing hair loss and promoting hair growth.

[0062] Comparative Example 5: A traditional Chinese medicine granule for preventing hair loss and promoting hair growth, which is different from Example 1 in that the weight portion of oligomannose (MOS) is 60 parts, and the weight ratio of nano-Rhodiola rosea extract to oligomannose (MOS) is 14:1.

[0063] Comparative Example 6: A traditional Chinese medicine granule for preventing hair loss and promoting hair growth, which is different from Example 3 in that the weight portion of oligomannose (MOS) is 100 parts, and the weight ratio of nano-Rhodiola rosea extract to oligomannose (MOS) is 9:1.

[0064] Table 1: Raw material composition of the Chinese medicine granules described in Examples 1-3 and Comparative Examples 1-6

[0065]

[0066]

[0067] Test Example 1: Animal hair growth promotion test

[0068] (1) Test samples: the hair loss prevention and hair growth Chinese herbal granules of Examples 1-3 and Comparative Examples 1-6.

[0069] (2) Animal treatment: Experimental animals: Purchase 100 rats of similar weight and hair growth, half male and half female. Under light anesthesia, heat and melt a mixture of rosin and paraffin wax in a mass ratio of 1:1 and apply it to the back of the mice. After it solidifies and hardens, gently peel it off. The hair removal area of each mouse is 3×3 cm. 2 .

[0070] (3) Grouping: The depilated mice were randomly divided into 10 groups, with 10 mice in each group. Each of the Chinese medicine granules to be tested in Examples 1-3 and Comparative Examples 1-6 in step (1) was gavaged once at a fixed time every day, with a gavage dose of 1g / kg.BW. The control group was gavaged with normal saline of equal mass for 28 consecutive days. On the 29th day, 10 long hairs were randomly selected from the test area of each group of experimental mice, and their lengths were measured with a vernier caliper. At the same time, the hairs in the test area were collected and weighed. The test results were statistically analyzed, and the results were averaged. The test results are shown in Table 2.

[0071] Table 2: Test results of animal hair growth promotion test

[0072]

[0073]

[0074] Test Example 2: Human hair loss prevention and growth test

[0075] (1) Test samples: the Chinese herbal granules in Examples 1-3 and Comparative Examples 1-6.

[0076] (2) Test subjects: 100 people aged 25-35 with hair loss, 10 people in each group. The test group took the test samples of Examples 1-3 and Comparative Examples 1-6 in step (1) once daily, 2 g / time, and the control group took a placebo for 28 consecutive days. During the test period, they did not change their original eating habits and ate a normal diet. Therapeutic effect evaluation criteria: effective rate = (number of effective patients / 10) × 100%; effective rate (%) = [(number of effective patients + number of effective patients) / 10] × 100%. The test results are shown in Table 3.

[0077] Table 3: Test results of human anti-hair loss and hair growth test

[0078]

[0079] Through the analysis of the test results of Experimental Example 1 and Experimental Example 2, it can be seen that: from the comparison of the test results of Comparative Example 1 and Example 3, it can be seen that the animal pancreatic extract is obtained by using protease inhibitors, and its application in traditional Chinese medicine granules for preventing hair loss and promoting hair growth can bring good anti-hair loss and hair growth effects.

[0080] The present invention adopts oligomannose (MOS) to promote the proliferation of intestinal probiotics, increase the production of short-chain fatty acids, and indirectly reduce the level of systemic oxidative stress. At the same time, its degradation products can inhibit the colonization of pathogenic bacteria such as Malassezia and inhibit the production of reactive oxygen species by harmful bacteria; and nano-rhodiola rosea extract directly enhances the activity of antioxidant enzymes, reducing the damage of oxidative stress to hair follicle stem cells. Oligomannose (MOS) and nano-rhodiola rosea extract of a specific weight ratio cooperate with each other, nano-rhodiola rosea extract enhances the endogenous antioxidant capacity of cells, oligomannose (MOS) reduces exogenous stressors, and forms an "inside and outside double barrier" to protect the hair follicle microenvironment. As shown in the test results of Comparative Example 2 and Example 2, adding oligomannose (MOS) and nano-rhodiola rosea extract to the anti-hair loss and hair growth Chinese medicine granules can play a role in promoting hair growth and preventing and treating hair loss. Moreover, by comparing Comparative Example 5 with Example 1 and Comparative Example 6 with Example 3, it can be seen that the nano-Rhodiola rosea extract and oligomannose (MOS) have the best synergistic effect within a specific weight ratio range, and can achieve the greatest anti-hair loss and hair growth effects.

[0081] The present invention also uses a rapid cooling method to reduce the reaction product to the freeze-drying temperature and freeze-dry it. The pulverization temperature is controlled during the heating process to room temperature. A step-by-step heating method is used to slowly heat the extract to room temperature. This controlled heating and cooling process can better preserve the pharmaceutical activity of the Rhodiola rosea extract, further facilitating its regulation of hair follicle stem cell autophagy and prolonging the hair follicle growth phase. It also better preserves the active ingredients of antioxidant enzymes, scavenges reactive oxygen species in hair follicle cells, and enhances hair follicle cell mitochondrial function. A comparison of the results of Comparative Example 3 with Example 2 shows that by using the cooling rate described in the present invention to freeze-dry the product and then slowly heating it to room temperature using a step-by-step heating method, the activity of the Rhodiola rosea extract can be better preserved, achieving better hair loss prevention and hair growth efficacy.

[0082] The present invention encapsulates nano-Rhodiola rosea extract and oligomannose (MOS) in chitosan / sodium alginate microcapsules, achieving an encapsulation efficiency of ≥90%, an intestinal release rate ≤3%, and a scalp-targeted release ≥80% for the traditional Chinese medicine granule. This achieves co-delivery and enhances the scalp targeting of the traditional Chinese medicine granule. Comparing the experimental results of Comparative Example 4 with those of Example 2 shows that encapsulating nano-Rhodiola rosea extract and oligomannose (MOS) in chitosan / sodium alginate microcapsules enhances the scalp targeting of the traditional Chinese medicine granule, achieving better hair loss prevention and hair growth effects.

[0083] In summary, the various herbs in the Chinese herbal granules of the present invention complement each other, have the effects of invigorating qi and nourishing blood, promoting blood circulation and promoting hair growth, and have a rapid onset of action, which can accelerate hair growth. Pharmacological efficacy experiments have shown that the Chinese herbal granules of the present invention have significant effects in preventing hair loss and promoting hair growth, without toxic side effects, and have good clinical application prospects.

[0084] The applicant declares that the present invention is illustrated by the above-described embodiments, but the present invention is not limited to the above-described embodiments. This does not mean that the present invention must rely on the above-described embodiments in order to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent replacements for raw materials in the present invention, additions of auxiliary ingredients, and selection of specific methods, etc., fall within the scope of protection and disclosure of the present invention.

[0085] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the scope of protection of the present invention.

[0086] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A Chinese herbal granule for preventing hair loss and promoting hair growth, characterized in that: The Chinese medicine granules are composed of the following components in parts by weight: 800-1000 parts of nano-Rhodiola rosea extract, 100-200 parts of Ligustrum lucidum fruit, 20-30 parts of astragaloside IV, 20-30 parts of Chuanxiong rhizome, 0.03-0.05 parts of selenomethionine, 50-100 parts of oligomannose MOS, 60-90 parts of chitosan / sodium alginate conjugate, and 30-60 parts of animal pancreas extract; The preparation method of the nano-rhodiola rosea extract comprises the following steps: placing rhodiola rosea in distilled water, adding cellulase, adjusting the pH of the system to 4.5-5.5, heating to 45°C-65°C for reaction for 1-2 hours, cooling the reaction product to a freeze-drying temperature at a cooling rate of 80-100°C / min, freeze-drying at -60--80°C for 12-36 hours, ultrafinely grinding the product to 300-500 nm in a liquid nitrogen environment at -60--80°C, and heating the temperature from the grinding temperature to room temperature, wherein the specific heating process from the grinding temperature to room temperature is as follows: heating the grinding and drying temperature to -40°C, keeping the temperature for 30 minutes, heating from -40°C to -10°C, keeping the temperature for 50 minutes, and returning from -10°C to room temperature, to obtain the nano-rhodiola rosea extract; The preparation method of the animal pancreatic extract comprises the following steps: obtaining fresh animal pancreas, adding protease inhibitors to the pancreas, crushing the pancreas into a paste, adding a protein extract, stirring and centrifuging the mixture at a low temperature of 0-4°C, collecting the supernatant, and filtering the supernatant with a microfiltration membrane to remove impurities, thereby obtaining the animal pancreatic extract.

2. A Chinese medicine granule according to claim 1, characterized in that: The weight ratio of the nano-rhodiola rosea extract to the oligomannosaccharide MOS is 10-12:

1.

3. A method for preparing the Chinese medicine granules according to claim 1, characterized in that: The Chinese medicine granules are prepared by the following steps: (1) Preparation of nano-Rhodiola rosea extract: placing Rhodiola rosea in distilled water, adding cellulase, adjusting the pH of the system to 4.5-5.5, heating to 45°C-65°C for reaction for 1-2 hours, cooling the reaction product to a freeze-drying temperature at a cooling rate of 80-100°C / min, freeze-drying at -60--80°C for 12-36 hours, ultrafinely grinding to 300-500 nm in a liquid nitrogen environment at -60--80°C, and heating from the grinding temperature to room temperature, wherein the specific heating process from the grinding temperature to room temperature is: heating the grinding and drying temperature to -40°C, keeping warm for 30 minutes, -40°C to -10°C, keeping warm for 50 minutes, and -10°C to room temperature, to obtain the nano-Rhodiola rosea extract; (2) Preparing an animal pancreatic extract: obtaining a fresh animal pancreas, adding a protease inhibitor to the pancreas, crushing the pancreas into a paste, then adding a protein extract, stirring and centrifuging at a low temperature of 0-4°C, collecting the supernatant, and filtering the supernatant with a microfiltration membrane to remove impurities, thereby obtaining the animal pancreatic extract; (3) taking a certain amount of nano-Rhodiola rosea extract and oligomannose (MOS) and mixing them evenly, adding the mixture to a sodium alginate aqueous solution to form a suspension, electrostatically spraying the suspension, dropping it into a mixed solution formed by chitosan and calcium chloride, solidifying it into microspheres, and separating the microspheres to obtain a sustained-release microcapsule component; (4) A certain amount of sustained-release microcapsule components, Ligustrum lucidum fruit, astragaloside IV, Chuanxiong rhizome, selenomethionine, and animal pancreatic extract are mixed evenly to obtain a Chinese medicine granule for preventing hair loss and promoting hair growth.

4. A preparation method according to claim 3, characterized in that: The microfiltration membrane is a 0.45 micron microfiltration membrane.

5. A preparation method according to claim 3, characterized in that: The animal pancreas is a cow or rabbit pancreas.

6. A preparation method according to claim 3, characterized in that: In step (1), cellulase is added to adjust the pH of the system to 5-5.

5.

7. A preparation method according to claim 6, characterized in that: In step (1), after adjusting the pH of the system, the mixture is heated to 55°C-65°C and reacted for 1-2 hours.

8. A preparation method according to claim 7, characterized in that: In step (1), after adjusting the pH of the system, the mixture is heated to 55°C-65°C and reacted for 1.5 hours.

9. A preparation method according to claim 3, characterized in that: The protease inhibitor is phenylmethylsulfonyl fluoride (PMSF), and the protein extract is phosphate buffer solution or physiological saline.

10. Use of the hair loss prevention and hair growth Chinese medicine granules according to claim 1, characterized in that: The anti-hair loss and hair growth Chinese medicinal granules are used in hair growth and prevention of hair loss.

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