Tea, tea powder, tea bag and application thereof

Abstract

The invention relates to the technical field of traditional Chinese medicine tea, in particular to tea, tea powder, a tea bag and application thereof.The tea is prepared from, by weight, 40-50 parts of amaranthus trifoliatus, 8-12 parts of cacumen biotae, 4.8-7.2 parts of aloe and 8-12 parts of semen plantaginis, the amaranthus trifoliatus is used as a main medicine, and heat clearing, diuresis promoting and detoxification are mainly achieved; the cacumen biotae is used for cooling blood and stopping bleeding, preventing phlegm from forming and stopping coughing, enhancing the heat clearing effect and preventing blood collaterals from being injured by heat; the aloe is used for clearing heat, purging fire and relaxing the bowels, so that damp and heat are eliminated from urination and defecation; the semen plantaginis has the effects of inducing diuresis, treating stranguria, excreting dampness, stopping diarrhea and guiding downward The four medicines are clear in main, auxiliary and guide, have a synergistic effect, have the effects of clearing heat, promoting diuresis, treating stranguria and inducing diuresis, and aim at the pathogenesis core of damp-heat accumulation.

Description

Technical Field

[0001] The present invention relates to the technical field of Chinese medicinal tea, in particular to tea, tea powder and tea bag and applications thereof. Background Art

[0002] Damp-heat accumulation is a common syndrome in Traditional Chinese Medicine (TCM). It can lead to poor urination in the lower abdomen, causing symptoms such as painful and difficult urination, dribbling, frequent and urgent urination, urinary tract infections (UTIs), and edema. Modern medical conditions such as localized urinary tract infections, prostatitis, urethral syndrome, and functional edema may exhibit these symptoms.

[0003] Currently, Western antibiotics or chemical diuretics are commonly used in clinical treatment, but they are prone to drug resistance and side effects (such as electrolyte imbalance and gastrointestinal discomfort). Traditional Chinese medicine also has a number of prescriptions (such as Bazheng Powder and Daochi Powder) or single herbs (such as Centella asiatica and Lygodium japonicum) with heat-clearing and diuretic effects. However, existing prescriptions may have complex compositions, poor taste, tedious decoction, or unclear mechanisms of action. Single herbs also have limited efficacy and lack specificity.

[0004] Therefore, it is necessary to develop a new type of Chinese medicine composition with a refined formula, clear efficacy, easy preparation and convenient consumption (such as brewing as tea), so as to safely and effectively relieve abnormal urination and related edema symptoms caused by damp-heat accumulation, and provide patients with a convenient, natural and healthy choice. Summary of the Invention

[0005] In order to solve the above technical problems, the present invention provides tea, tea powder and tea bag and applications thereof.

[0006] In a first aspect, the present invention provides a tea composed of the following raw materials in parts by weight: 40-50 parts of Amaranthus truncatus, 8-12 parts of Platycladus orientalis leaves, 4.8-7.2 parts of aloe vera, and 8-12 parts of Plantago seeds.

[0007] Preferably, it is composed of the following raw materials in parts by weight: 42-48 parts of Amaranthus truncatula, 9-11 parts of Platycladus orientalis leaves, 5.4-6.6 parts of aloe vera, and 9-11 parts of Plantago seeds.

[0008] Preferably, the weight ratio of the raw materials is: Amaranthus truncatula: Platycladus orientalis leaves: Aloe vera: Plantago seeds = 9.0:2.0:1.2:2.0.

[0009] Preferably, it is composed of the following raw materials in parts by weight: 45 parts of Amaranthus truncatula, 10 parts of Platycladus orientalis leaves, 6 parts of aloe vera, and 10 parts of Plantago seeds.

[0010] In a second aspect, the present invention provides a method for preparing the tea according to the first aspect, comprising the following steps: S51, taking the raw materials of Amaranthus truncatus, Platycladus orientalis, Aloe vera and Plantago asiatica in the formula amount; S52, adding 5-15 times the total weight of the raw materials into water for decoction; S53, start timing from the time the water boils and cook for 10-20 minutes; S54, filter, and take the filtrate to obtain the tea beverage.

[0011] Preferably, the decocting time in step S53 is 15 minutes.

[0012] In a third aspect, the present invention provides a method for preparing the tea powder of the tea according to the first aspect, comprising the following steps: S71. Take the raw materials of Amaranthus truncatus, Platycladus orientalis leaves, Aloe vera and Plantago asiatica in the formulated amounts; S72, drying at 40-70°C to a moisture content of 6-8 wt%, and then crushing to 60-120 mesh; S73. Evenly mix the crushed raw materials to obtain tea powder.

[0013] In a fourth aspect, the present invention provides a tea bag comprising the tea powder prepared by the method according to the third aspect, wherein the tea bag contains 2.5-3.5 g of the tea powder, and the tea bag is made of permeable filter paper or tea bag cloth.

[0014] In a fifth aspect, the present invention provides a use of the tea according to the first aspect, the tea prepared according to the second aspect, or the tea bag according to the fourth aspect in the preparation of medicines, health foods or beverages.

[0015] In a sixth aspect, the present invention provides a use of the tea as described in the first aspect, the tea prepared as described in the second aspect, or the tea bag as described in the fourth aspect for preparing a medicine or health food for improving or treating painful urination, dribbling, frequent and urgent urination, urinary tract infection or edema caused by accumulation of damp heat.

[0016] Compared with the prior art, the present invention has the following beneficial effects: (1) The present invention uses Amaranthus tricolor as the main ingredient, which mainly clears heat, promotes dampness and detoxifies. It is supplemented by Platycladus orientalis leaves, which cool the blood, stop bleeding, resolve phlegm and relieve cough, enhance the heat-clearing effect, and prevent heat from damaging blood vessels. It is supplemented by Aloe vera, which clears heat, purges fire, and relieves constipation, allowing dampness and heat to be eliminated through defecation and urination. It is supplemented by Plantago seed, which promotes diuresis, relieves stranguria, and stops diarrhea, guiding dampness and heat downward. The four herbs are clearly distinguished in their main and auxiliary roles, and work synergistically to achieve the effects of clearing heat, promoting dampness, relieving stranguria and promoting diuresis, targeting the core pathogenesis of damp-heat accumulation.

[0017] (2) The tea prepared by the present invention can effectively improve the symptoms of painful urination, poor urination, frequent and urgent urination, urinary tract infection and edema caused by damp-heat accumulation.

[0018] (3) The present invention adopts a water decoction method: the decoction time is short, volatile or heat-sensitive active ingredients are retained to the greatest extent, and energy consumption and ingredient destruction are reduced. The resulting tea has a pleasant taste and is easy to take. The tea powder method uses low-temperature drying and precise grinding technology, which is easy to operate, retains active ingredients to the greatest extent, and is convenient for storage and transportation. DETAILED DESCRIPTION

[0019] The following will clearly and completely describe the technical solutions of the present invention in conjunction with specific embodiments. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0020] Example 1 This embodiment provides a tea composed of the following raw materials in parts by weight: 50 parts of Amaranthus truncatus, 8 parts of Platycladus orientalis leaves, 7.2 parts of aloe vera, and 12 parts of Plantago seeds.

[0021] This embodiment provides a method for preparing tea, comprising the following steps: S1. Take the raw materials of Amaranthus truncatus, Platycladus orientalis leaves, Aloe vera and Plantago asiatica in the prescribed amount; S2, add 10 times the total weight of the raw materials into water and boil; S3, start timing from the time the water boils and cook for 15 minutes; S4. Filter and take the filtrate to obtain the tea beverage.

[0022] Example 2 This embodiment provides a tea composed of the following raw materials in parts by weight: 42 parts of Amaranthus truncatus, 11 parts of Platycladus orientalis leaves, 5.4 parts of aloe vera, and 9 parts of Plantago seeds.

[0023] This embodiment provides a method for preparing tea, comprising the following steps: S1. Take the raw materials of Amaranthus truncatus, Platycladus orientalis leaves, Aloe vera and Plantago asiatica in the prescribed amount; S2, add 5 times the total weight of the raw materials into water and boil; S3, start timing from the time the water boils and cook for 20 minutes; S4. Filter and take the filtrate to obtain the tea beverage.

[0024] Example 3 (based on claim 3) This embodiment provides a tea composed of the following raw materials in parts by weight: 45 parts of Amaranthus truncatus, 10 parts of Platycladus orientalis leaves, 6 parts of aloe vera, and 10 parts of Plantago seeds.

[0025] This embodiment provides a method for preparing tea, comprising the following steps: S1. Take the raw materials of Amaranthus truncatus, Platycladus orientalis leaves, Aloe vera and Plantago asiatica in the prescribed amount; S2, add 15 times the total weight of the raw materials into water and boil; S3, start timing from the time the water boils and cook for 10 minutes; S4. Filter and take the filtrate to obtain the tea beverage.

[0026] Example 4 (based on claim 4) This embodiment provides a tea composed of the following raw materials in parts by weight: 45 parts of Amaranthus truncatus, 10 parts of Platycladus orientalis leaves, 6 parts of aloe vera, and 10 parts of Plantago seeds.

[0027] This embodiment provides a method for preparing tea, comprising the following steps: S1. Take the raw materials of Amaranthus truncatus, Platycladus orientalis leaves, Aloe vera and Plantago asiatica in the prescribed amount; S2, add 8 times the total weight of the raw materials into water and boil; S3, start timing from the time the water boils and cook for 15 minutes; S4. Filter and take the filtrate to obtain the tea beverage.

[0028] Example 5 This embodiment provides a tea composed of the following raw materials in parts by weight: 45 parts of Amaranthus truncatus, 10 parts of Platycladus orientalis leaves, 6 parts of aloe vera, and 10 parts of Plantago seeds.

[0029] This embodiment provides a method for preparing tea powder, comprising the following steps: S1. Take the raw materials of Amaranthus truncatus, Platycladus orientalis leaves, Aloe vera and Plantago asiatica in the prescribed amount; S2, drying at 60°C to a moisture content of 7 wt%, and then crushing to 90 mesh; S3. Evenly mix the crushed raw materials to obtain tea powder.

[0030] This embodiment provides a method for preparing a tea bag, comprising the steps of: taking 3.0 g of tea powder, putting it into a small tea bag made of non-woven tea bag cloth, and sealing it to obtain a tea bag.

[0031] Comparative Example 1 Compared with Example 4, this comparative example provides a tea composed of the following raw materials in parts by weight: 10 parts of Platycladus orientalis leaves, 6 parts of aloe vera, and 10 parts of Plantago seeds, and the remaining steps are the same as those of Example 4.

[0032] Comparative Example 2 Compared with Example 4, this comparative example provides a tea composed of the following raw materials in parts by weight: 35 parts of Amaranthus truncatus, 15 parts of Platycladus orientalis leaves, 8 parts of aloe vera, and 7 parts of Plantago seeds. The remaining steps are the same as those in Example 4.

[0033] Comparative Example 3 Compared with Example 4, this comparative example provides a tea composed of the following raw materials in parts by weight: 45 parts of Amaranthus truncatus, 10 parts of Platycladus orientalis leaves, 6 parts of aloe vera, 10 parts of Plantago seeds, and 15 parts of Lysimachia chinensis. The remaining steps are the same as those in Example 4.

[0034] Comparative Example 4 Compared with Example 4, this comparative example provides a method for preparing tea, wherein water 3 times the total weight of the raw materials is added for decocting, and the boiling time is counted after the water boils. The decocting time is 60 minutes, and the filtrate is filtered to obtain a tea beverage.

[0035] The tea prepared by Examples 1 to 5 and Comparative Examples 1 to 4 was tested: Test method: Mouse diuresis test Animal grouping: 70 healthy male mice were randomly divided into 7 groups (n=10): normal control group, model control group, positive drug control group, Example 1 group, Example 4 group, Comparative Example 1 group, and Comparative Example 4 group.

[0036] Modeling: Except for the normal control group, mice in other groups were subcutaneously injected with 0.9% NaCl solution at 0.5 mL / 10 g to establish a water load model.

[0037] Dosing: 30 minutes after modeling, each dosing group was gavaged with the corresponding drug solution or normal saline (0.2 mL / 10 g). The positive drug group was administered Sanjin Tablet solution (converted to the human equivalent dose). Examples 1 and 4 and Comparative Examples 1 and 4 were administered with the corresponding prepared beverage concentrate (equivalent to twice the original concentration). The normal and model groups were administered with an equal volume of normal saline.

[0038] Observation indicators: Mice were placed in metabolic cages with filter paper at the bottom, and the total urine volume within 0-6 hours after administration was collected.

[0039] Statistical analysis: Data were expressed as mean ± standard deviation, and one-way analysis of variance (ANOVA) was used for comparison among groups, with P < 0.05 considered significant.

[0040] Test method 2: in vitro antibacterial test Strain and culture medium: Standard uropathogenic Escherichia coli strain, nutrient broth culture medium.

[0041] Preparation of medicinal solutions: Prepare concentrated aqueous decoctions (1 g crude drug / mL) of Example 3, Example 4, and Comparative Example 3, and filter them aseptically. Prepare the concentrated solution of Comparative Example 2.

[0042] MIC determination: The minimum inhibitory concentration (MIC) of each group of tea samples against Escherichia coli was determined by microbroth dilution method.

[0043] Results: The MIC is the lowest drug concentration at which no bacterial growth is observed by naked eye. Record the MIC value (mg crude drug / mL) for each group. The lower the MIC value, the stronger the antibacterial effect.

[0044] Test method: Rat carrageenan paw swelling test Animal grouping: 56 healthy male SD rats were randomly divided into 7 groups (n=8): normal control group (no treatment), model control group, positive drug control group (such as hydrochlorothiazide), Example 2 group, Example 4 group, Comparative Example 1 group, and Comparative Example 2 group.

[0045] Dosing: Each group received the corresponding drug solution or normal saline (1 mL / 100 g) orally once daily for 3 consecutive days. The dose of the positive drug was converted to the human equivalent dose. The corresponding beverage concentrate was administered in the Examples and Comparative Examples.

[0046] Modeling and Measurements: One hour after the last administration, all rats, except the control group, were subcutaneously injected with 0.1 mL of 1% carrageenan solution into the left hind paw to induce inflammation. The volume of the left and right hind paws was measured using the water displacement method before (0 h) and at 1, 2, 3, 4, and 6 h after inflammation.

[0047] Calculate swelling: Swelling (mL) = Post-inflammation plantar volume - Pre-inflammation plantar volume. Calculate the average swelling and swelling inhibition rate at each time point.

[0048] Swelling inhibition rate (%) = [(average swelling degree of model control group - average swelling degree of drug-treated group) / average swelling degree of model control group] × 100% Statistical analysis: Data were expressed as mean ± standard deviation, and one-way analysis of variance (ANOVA) was used for comparison among groups, with P < 0.05 considered significant.

[0049] Test method: Yeast-induced fever model in rats (antipyretic) Animal grouping: 56 healthy male SD rats were randomly divided into 7 groups (n=8): normal control group, fever model control group, positive drug control group, Example 3 group, Example 4 group, Comparative Example 1 group, and Comparative Example 3 group.

[0050] Modeling: Except for the normal control group, the rats in the other groups were subcutaneously injected with 20% dry yeast suspension (10 mL / kg) on the back to establish the fever model.

[0051] Dosing: 16 hours after modeling, measure basal body temperature. Subsequently, each dosing group received oral administration of the corresponding drug solution or saline (1 mL / 100 g). The dose of the positive drug was converted to the human equivalent dose. The corresponding beverage concentrate was administered in the Examples and Comparative Examples.

[0052] Body temperature measurement: The body temperature of rats was measured with an electronic rectal thermometer 1h, 2h, 3h, 4h, 5h, and 6h after administration.

[0053] Calculation of body temperature change: Body temperature change (℃) = body temperature at measurement - basal body temperature.

[0054] Statistical analysis: Data are expressed as mean ± standard deviation. One-way analysis of variance (ANOVA) was used for comparison between groups, with P < 0.05 as the difference. Test method 5: Mouse bacterial cystitis model (simulating urinary tract infection).

[0055] Animal grouping: 70 female mice were randomly divided into 7 groups (n=10): normal control group, infection model control group, positive drug control group, Example 5 tea bag group, Example 4 group, Comparative Example 1 group, and Comparative Example 4 group.

[0056] Modeling: Except for the normal control group, mice were injected with 0.05 mL of bacterial suspension (containing 10 ^8 CFU / mL uropathogenic Escherichia coli).

[0057] Dosing: 24 hours after modeling, administration began. The drug-treated group received oral gavage with the corresponding drug solution or normal saline (0.2 mL / 10 g) twice daily for 5 consecutive days. The positive drug was dosed according to the instructions. The Example 5 group received oral gavage with tea bags soaked in boiling water (equivalent to the decoction concentration of Example 4) and cooled.

[0058] Sample collection and observation: 24 hours after the last administration, mice were sacrificed and bladder tissue was obtained aseptically.

[0059] Pathological examination (semi-quantitative): Part of the bladder tissue was fixed with 10% formalin, embedded in paraffin, and stained with hematoxylin and eosin. Inflammatory cell infiltration, mucosal damage, edema, etc. were observed under a microscope and an inflammation score was calculated (0-3 points or none, mild, moderate, severe).

[0060] Bacterial culture count: After a portion of the bladder tissue is homogenized, it is diluted with normal saline and inoculated onto a blood agar plate. The plate is cultured at 37°C for 24 hours and the number of bacterial colonies per gram of bladder tissue (CFU / g) is counted.

[0061] The test results of the above tests are shown in Table 1 Table 1

[0062]

[0063] Among them, (* represents P < 0.05, ** represents P < 0.01 vs. model control group or designated group) Test conclusion: (1) Example 4 had the best overall performance and was significantly superior to the other groups in terms of diuresis, antibacterial, anti-inflammatory, anti-inflammatory, and anti-urinary tract infection.

[0064] (2) The effect of control example 1, which lacked Amaranthus truncatula, was significantly reduced in all tests.

[0065] (3) Long decoction time or insufficient water (high MIC value in antibacterial test) will reduce activity.

[0066] (4) Example 5 has similar effects to the decoction (Example 4) in terms of anti-infection and pathological improvement. (5) In terms of diuretic effect, the best group was Example 4, lacking Amaranthus chinensis (Comparative Example 1): urine output ≈ model group, proving that Amaranthus chinensis was the main active ingredient, long-term boiling: the effect was weaker than that of Example 4, indicating that the heat-sensitive components were destroyed.

[0067] (6) In terms of antibacterial effect (MIC value), the best group was Example 4 (MIC = 35.7 mg / mL). The MIC in Comparative Example 2 was as high as 78.5 mg / mL, indicating the weakest antibacterial activity. The MIC in Comparative Example 3 increased (67.3 mg / mL). The MIC in Example 3 was slightly higher than that in Example 4, suggesting that excessive water may dilute the active ingredient. Although Centella asiatica itself is believed to have a diuretic and stranguria-relieving effect, the addition of Centella asiatica interfered with or diluted the synergistic effect, resulting in a decrease in the overall effect.

[0068] (7) In terms of anti-inflammatory and detumescent effects, the best group was Example 4 (inhibition rate 52.7%, P < 0.01), while the inhibition rate of Comparative Example 1 was only 22.5%. Comparative Example 2 had the worst effect, and the effect of Example 2 was significant but weaker than that of Example 4.

[0069] In terms of antipyretic effect, the optimal group is Example 4. In Comparative Example 1, ΔT≈model group. Although Comparative Example 3 is effective, the effect is general, which interferes with the synergistic effect.

[0070] In terms of anti-urinary tract infection effect, bacterial load (Log10 CFU / g), Examples 4 and 5 significantly reduced the infection load, and Examples 4 and 5 significantly alleviated tissue damage.

[0071] The specific embodiments further describe the present invention, but it should be understood that the specific description here should not be construed as limiting the essence and scope of the present invention. Various modifications made to the above embodiments by ordinary technicians in this field after reading this specification are all within the scope of protection of the present invention.

Claims

1. A tea, characterized in that The invention is composed of the following raw materials in parts by weight: 40-50 parts of Amaranthus truncatus, 8-12 parts of Platycladus orientalis leaves, 4.8-7.2 parts of aloe vera and 8-12 parts of Plantago seeds.

2. The tea according to claim 1, characterized in that The invention is composed of the following raw materials in parts by weight: 42-48 parts of Amaranthus truncatus, 9-11 parts of Platycladus orientalis leaves, 5.4-6.6 parts of aloe vera and 9-11 parts of Plantago seeds.

3. The tea according to claim 1 or 2, characterized in that The weight ratio of the raw materials is: Amaranthus truncatus: Platycladus orientalis leaves: Aloe vera: Plantago seeds = 9.0:2.0:1.2:2.

0.

4. The tea according to claim 1, 2 or 3, characterized in that The invention is composed of the following raw materials in parts by weight: 45 parts of Amaranthus truncatus, 10 parts of Platycladus orientalis leaves, 6 parts of aloe vera and 10 parts of Plantago seeds.

5. A method for preparing the tea according to any one of claims 1 to 4, characterized in that: The following steps are involved: S51, taking the raw materials of Amaranthus truncatus, Platycladus orientalis, Aloe vera and Plantago asiatica in the formula amount; S52, adding 5-15 times the total weight of the raw materials into water for decoction; S53, start timing from the time the water boils and cook for 10-20 minutes; S54, filter, and take the filtrate to obtain the tea beverage.

6. The method according to claim 5, characterized in that The decoction time in step S53 is 15 minutes.

7. A method for preparing tea powder according to any one of claims 1 to 4, characterized in that: The following steps are involved: S71. Take the raw materials of Amaranthus truncatus, Platycladus orientalis leaves, Aloe vera and Plantago asiatica in the formulated amounts; S72, drying at 40-70°C to a moisture content of 6-8 wt%, and then crushing to 60-120 mesh; S73. Evenly mix the crushed raw materials to obtain tea powder.

8. A tea bag comprising tea powder prepared according to the method of claim 7, characterized in that: The tea bag contains 2.5-3.5 g of the tea powder, and the tea bag is made of permeable filter paper or tea bag cloth.

9. Use of the tea according to any one of claims 1 to 4, the tea prepared according to any one of claims 5 to 6, or the tea bag according to claim 8 in preparing medicines, health foods or beverages.

10. Use of the tea according to any one of claims 1 to 4, the tea prepared according to any one of claims 5 to 6, or the tea bag according to claim 8 for preparing a medicine or health food for improving or treating painful urination, dribbling, frequent and urgent urination, urinary tract infection or edema caused by accumulation of damp heat.

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