Traditional Chinese medicine composition for treating salpingitis of laying hens as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention provides a traditional Chinese medicine composition for treating salpingitis of laying hens. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 25-35 parts of dandelion, 15-25 parts of motherwort, 10-20 parts of semen plantaginis, 10-20 parts of fructus forsythiae, 10-20 parts of radix salviae miltiorrhizae and 5-15 parts of rhizoma atractylodis macrocephalae. The invention further provides a corresponding preparation method and application. The traditional Chinese medicine composition has a good treatment effect on laying hens, and compared with western medicines, the traditional Chinese medicines are mild in property and small in side effect.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating laying hen salpingitis, and a preparation method and application thereof. Background Art

[0002] Salpingitis in laying hens is a common disease in laying hen farming, characterized by abnormal egg production, decreased egg quality, and lethargy. In severe cases, it can lead to death. The etiology of this disease is complex, including pathogenic microbial infection, improper husbandry and management, and stress. Frequent sapping of sapping tube inflammation causes significant economic losses to laying hen operations, impacting the healthy development of the industry.

[0003] At present, farms generally use antibiotics to treat oviduct inflammation in laying hens. Antibiotics are effective quickly, but they have certain side effects, and long-term use will produce certain drug resistance. Summary of the Invention

[0004] In order to solve the problems existing in the prior art, the present invention provides a traditional Chinese medicine composition for treating salpingitis in laying hens. The traditional Chinese medicine composition has a good therapeutic effect on laying hens, and compared with Western medicine, traditional Chinese medicine has mild properties and few side effects.

[0005] The first object of the present invention is to provide a traditional Chinese medicine composition for treating salpingitis in laying hens, wherein the traditional Chinese medicine composition is composed of the following components in parts by weight: 25-35 parts of dandelion, 15-25 parts of motherwort, 10-20 parts of plantain seeds, 10-20 parts of forsythia, 10-20 parts of salvia miltiorrhiza, and 5-15 parts of atractylodes.

[0006] Preferably, the Chinese medicine composition is composed of the following components in parts by weight: 25 parts of dandelion, 22 parts of motherwort, 15 parts of plantain seeds, 18 parts of forsythia, 14 parts of salvia miltiorrhiza, and 12 parts of atractylodes.

[0007] Preferably, the Chinese medicine composition is composed of the following components in parts by weight: 35 parts of dandelion, 25 parts of motherwort, 20 parts of plantain seeds, 10 parts of forsythia, 10 parts of salvia miltiorrhiza, and 15 parts of atractylodes.

[0008] Preferably, the Chinese medicine composition is composed of the following components in parts by weight: 20 parts of dandelion, 24 parts of motherwort, 15 parts of plantain seeds, 18 parts of forsythia, 15 parts of salvia miltiorrhiza, and 12 parts of atractylodes.

[0009] Preferably, the Chinese medicine composition is composed of the following components in parts by weight: 30 parts of dandelion, 15 parts of motherwort, 10 parts of plantain seeds, 20 parts of forsythia, 20 parts of salvia miltiorrhiza, and 5 parts of atractylodes.

[0010] Preferably, the Chinese medicine composition is composed of the following components in parts by weight: 26 parts of dandelion, 18 parts of motherwort, 16 parts of plantain seeds, 14 parts of forsythia, 15 parts of salvia miltiorrhiza, and 10 parts of atractylodes.

[0011] The fourth object of the present invention is to provide a method for preparing the above-mentioned Chinese medicine composition, which comprises mixing the raw materials in the prescribed amounts and decocting them in water to obtain a decoction.

[0012] The fifth object of the present invention is to provide a method for preparing the above-mentioned traditional Chinese medicine composition, wherein the raw materials of the formula amount are mixed, dried, and ultrafinely ground to obtain granules.

[0013] The sixth object of the present invention is to provide the use of the above-mentioned traditional Chinese medicine composition in the preparation of medicine or feed for treating laying hen salpingitis.

[0014] The seventh object of the present invention is to provide a medicine, the active ingredient of which is the above-mentioned traditional Chinese medicine composition.

[0015] The TCM prescription of the present invention is explained in Table 1.

[0016] Table 1

[0017] traditional Chinese medicine Nature and flavor Compatibility dandelion Bitter, sweet, cold, liver and stomach meridians Main drug: clearing away heat and detoxifying, anti-inflammatory and swelling Motherwort Pungent and slightly cold, liver meridian Assistant medicine: promote blood circulation and menstruation, improve microcirculation Plantago Sweet and cold, kidney, liver and lung meridians Assistant: diuresis, relieving stranguria, guiding dampness and heat downward Forsythia Bitter and slightly cold, lung and heart meridians Auxiliary drugs: clear away heat in the blood, dissipate stagnation and relieve pain Salvia miltiorrhiza Bitter and slightly cold, heart and liver meridians Assistant medicine: promote blood circulation and remove blood stasis, improve tissue blood supply Atractylodes macrocephala Sweet, bitter and warm, spleen and stomach meridian Assistant: Strengthen the spleen and dry out dampness, prevent coldness from damaging the stomach

[0018] This medicine primarily clears damp-heat, supplemented by promoting blood circulation and unblocking meridians, while also strengthening the spleen and tonifying the body's vital energy. Targeting the core pathogenesis of salpingitis, which is damp-heat and blood stasis, dandelion and forsythia clear heat and toxicity, plantago seed dissipates dampness and conducts heat downward, motherwort and salvia miltiorrhiza promote blood circulation and menstruation, and white atractylodes strengthens the spleen to eliminate the source of dampness. The entire formula combines both cold and warming properties, attacking and tonifying, aligning with the therapeutic principle of "treating symptoms in emergencies and addressing the root cause in chronic situations."

[0019] The traditional Chinese medicine composition of the present invention has a good therapeutic effect on laying hens, and compared with western medicine, the traditional Chinese medicine has mild properties and small side effects. DETAILED DESCRIPTION

[0020] The following examples are provided to facilitate a better understanding of the present invention, but are not intended to limit the present invention. The experimental methods in the following examples, unless otherwise specified, are conventional methods. The experimental materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent companies. The quantitative tests in the following examples were performed in triplicate, and the results were averaged.

[0021] The Chinese medicinal composition for treating laying hen salpingitis of the present invention is composed of the following components in parts by weight:

[0022] 25-35 parts of dandelions, 15-25 parts of motherwort, 10-20 parts of plantain seeds, 10-20 parts of forsythia, 10-20 parts of salvia miltiorrhiza, and 5-15 parts of atractylodes.

[0023] In the acute stage of laying hen salpingitis, 20-25 parts of honeysuckle can be added, and in the chronic stage, 25-30 parts of astragalus can be added.

[0024] The preparation method of the water decoction of the Chinese medicinal composition for treating laying hen salpingitis of the present invention is as follows:

[0025] After mixing the raw materials in the formula, add water and boil twice, for 1.5 hours and 1 hour respectively, combine the liquid medicines, adjust the total amount to 1000 parts by weight, stir evenly, package and sterilize.

[0026] The preparation method of the Chinese medicine composition granules for treating laying hen salpingitis of the present invention is as follows:

[0027] The raw materials of the formula amount are mixed, dried, and ultrafinely ground to obtain granules.

[0028] The method of using the Chinese medicine composition for treating laying hen salpingitis of the present invention is as follows:

[0029] Mix the decoction or granules into the drinking water of laying hens, one dose per day (one dose is: 25-35g of dandelion, 15-25g of motherwort, 10-20g of plantain seed, 10-20g of forsythia, 10-20g of salvia miltiorrhiza, 5-15g of atractylodes), 2ml per chicken, once a day, for 5-7 days.

[0030] The above-mentioned medicinal materials are all varieties included in the pharmacopoeia, with sufficient market supply. The average cost of raw materials per kilogram does not exceed 30 yuan, and they are suitable for use in large-scale farms.

[0031] Example 1

[0032] The Chinese medicine composition for treating laying hen salpingitis of the present embodiment is composed of the following components:

[0033] Dandelion 25g, Motherwort 22g, Plantago seed 15g, Forsythia suspensa 18g, Salvia miltiorrhiza 14g, Atractylodes macrocephala 12g.

[0034] The preparation method of the water decoction of the Chinese medicinal composition for treating laying hen salpingitis of the present invention is as follows:

[0035] After mixing the raw materials in the formula amount, add water and boil twice, for 1.5 hours and 1 hour respectively, combine the liquid medicines, adjust the total volume to 1000 ml, stir well, package and sterilize.

[0036] Mix the decoction into the drinking water of laying hens, 1 dose per day (25g of dandelion, 22g of motherwort, 15g of plantain seed, 18g of forsythia, 14g of salvia miltiorrhiza, 12g of atractylodes), 2ml per chicken, once a day for 5 consecutive days.

[0037] Comparative Example 1

[0038] The difference between this comparative example and Example 1 is that dandelion is replaced by codonopsis pilosula.

[0039] The remaining parameters, preparation methods and usage methods are the same as those in Example 1.

[0040] Example 2

[0041] The Chinese medicine composition for treating laying hen salpingitis of the present embodiment is composed of the following components:

[0042] Dandelion 35g, Motherwort 25g, Plantago seed 20g, Forsythia suspensa 10g, Salvia miltiorrhiza 10g, Atractylodes macrocephala 15g.

[0043] The preparation method and use method of the Chinese medicine composition of this embodiment are the same as those of Example 1.

[0044] Comparative Example 2

[0045] The difference between this comparative example and Example 2 is that Leonurus japonicus is replaced with mature rehmannia root.

[0046] The remaining parameters, preparation methods and usage methods are the same as those in Example 2.

[0047] Example 3

[0048] The Chinese medicine composition for treating laying hen salpingitis of the present embodiment is composed of the following components:

[0049] Dandelion 20g, Motherwort 24g, Plantago seed 15g, Forsythia suspensa 18g, Salvia miltiorrhiza 15g, Atractylodes macrocephala 12g.

[0050] The preparation method and use method of the Chinese medicine composition of this embodiment are the same as those of Example 1.

[0051] Comparative Example 3

[0052] The difference between this comparative example and Example 3 is that dandelion is replaced by honeysuckle, and motherwort is replaced by Panax notoginseng.

[0053] The remaining parameters, preparation methods and usage methods are the same as those in Example 3.

[0054] Example 4

[0055] The Chinese medicine composition for treating laying hen salpingitis of the present embodiment is composed of the following components:

[0056] Dandelion 30g, Motherwort 15g, Plantago 10g, Forsythia suspensa 20g, Salvia miltiorrhiza 20g, Atractylodes macrocephala 5g.

[0057] The preparation method and use method of the Chinese medicine composition of this embodiment are the same as those of Example 1.

[0058] Comparative Example 4

[0059] The difference between this comparative example and Example 4 is that it does not contain Salvia miltiorrhiza.

[0060] The remaining parameters, preparation methods and usage methods are the same as those in Example 4.

[0061] Example 5

[0062] The Chinese medicine composition for treating laying hen salpingitis of the present embodiment is composed of the following components:

[0063] Dandelion 26g, Motherwort 18g, Plantago seed 16g, Forsythia suspensa 14g, Salvia miltiorrhiza 15g, Atractylodes macrocephala 10g.

[0064] The preparation method and use method of the Chinese medicine composition of this embodiment are the same as those of Example 1.

[0065] Comparative Example 5

[0066] The difference between this comparative example and Example 5 is that it does not contain Plantago seeds.

[0067] The remaining parameters, preparation methods and usage methods are the same as those in Example 5.

[0068] Example 6

[0069] The Chinese medicine composition for treating laying hen salpingitis of the present embodiment is composed of the following components:

[0070] Dandelion 32g, Motherwort 22g, Plantago seed 14g, Forsythia suspensa 18g, Salvia miltiorrhiza 12g, Atractylodes macrocephala 8g.

[0071] The preparation method and use method of the Chinese medicine composition of this embodiment are the same as those of Example 1.

[0072] Comparative Example 6

[0073] The difference between this comparative example and Example 6 is that it does not contain Atractylodes macrocephala.

[0074] The remaining parameters, preparation methods and usage methods are the same as those in Example 6.

[0075] Application Example 1

[0076] 1. Test materials

[0077] 1.1 Experimental animals

[0078] A total of 520 56-week-old Hy-Line brown laying hens suffered from salpingitis, characterized by an increase in the number of broken and soft-shelled eggs and a significant decrease in egg production. Clinical veterinary diagnosis of salpingitis was conducted at Shuangsan Farm in Liangzhou District, Wuwei City.

[0079] 1.2 Investigational Drugs

[0080] The Chinese medicine compositions prepared in Examples 1-6 and Comparative Examples 1-6 of the present invention.

[0081] 2 Test methods

[0082] 2.1 Trial Grouping and Dosing

[0083] 520 laying hens with salpingitis included in the experiment were randomly divided into 13 groups, with 10 hens in each group and 4 replicates in each group. The Chinese medicine compositions prepared in Examples 1-6 and Comparative Examples 1-6 of the present invention were used respectively, and a blank control group was set up at the same time.

[0084] The administration method was as follows: each experimental group was given 2 ml / animal, once a day for 5 consecutive days, and the blank control group was not treated.

[0085] The various indicators were measured starting from the 5th day of treatment and continued for 5 consecutive days.

[0086] 2.2 Measurement indicators and methods

[0087] 2.2.1 Egg production performance

[0088] During the test period, the number of eggs laid by each group of chickens in the 7 days before administration and the number of eggs laid 7 days after the end of administration were counted, and the egg production rate and egg breakage rate of each test group were calculated. The results are shown in Table 2.

[0089] Egg production rate before drug administration = number of eggs produced 7 days before drug administration / 7.

[0090] Egg production rate after drug administration = number of eggs produced 7 days after the end of drug administration / 7.

[0091] Egg breaking rate before drug administration = number of broken eggs 7 days before drug administration / number of eggs produced 7 days before drug administration.

[0092] Egg breaking rate after drug administration = number of broken eggs 7 days after drug administration / number of eggs produced 7 days after drug administration.

[0093] 2.2.2 Egg quality determination

[0094] The egg quality (eggshell strength and thickness) of each group was tested 1 day before administration and 1 day after administration. The results are shown in Table 3.

[0095] 2.2.3 Inflammatory factor determination

[0096] On the day before administration and the day after administration, wing vein blood was collected, and the serum was separated to determine the inflammatory factors TNF-α and IFN-γ of each group of animals. The results are shown in Table 4.

[0097] 3 Test results

[0098] Table 2 Egg production performance test results

[0099]

[0100] As can be seen from Table 2, there was no significant difference in the egg production rate of each test group in the 7 days before administration. In the 7 days after the end of administration, the egg production rate of Examples 1-6 was significantly different compared with Comparative Examples 1, 3, 5 and the blank control group.

[0101] 7 days before administration, there was no significant difference in the egg rupture rate of each test group. 7 days after the end of administration, there was a significant difference between Examples 1-6 and Comparative Examples 1-6 and the blank control group, and there was also a significant difference between Comparative Examples 1-5 and Comparative Example 6.

[0102] Table 3 Egg quality determination results

[0103]

[0104]

[0105] As can be seen from Table 3, before administration, there was no significant difference in the eggshell strength of the eggs in each test group. After the administration, there was a significant difference between Examples 1-5 and Example 6; there was a significant difference between Example 6 and Comparative Examples 1-6; there was also a significant difference between each Example group, Comparative Example group and the blank control group, indicating that both the Example group and the Comparative Example group can improve the eggshell strength, but Examples 1-6 are better than the Comparative Examples.

[0106] Before administration, there was no significant difference in the eggshell thickness of the eggs in each test group. After administration, there was a significant difference between Example 1 and Examples 2-5; there was a significant difference between Examples 2-5 and Comparative Examples 1-6. There was also a significant difference between each Example group, Comparative Example and the blank control group, indicating that both the Example group and the Comparative Example group can increase the eggshell thickness, but Examples 1-6 are better than the Comparative Examples.

[0107] Table 4 Inflammatory factor detection results As can be seen from Table 4, before administration, there was no significant difference in TNF-α of the chickens in each test group. After administration, there was a significant difference between Examples 1-6 and Comparative Examples 1-6 and the blank control group, indicating that Examples 1-6 can significantly control the inflammatory factor TNF-α of chickens.

[0108] Before administration, there was no significant difference in IFN-γ levels in the chickens of each test group. After administration, there was a significant difference between Example 1-4 and Example 5; there was still a significant difference between Example 1-6 and Comparative Example 1-6 and the blank control group, indicating that Examples 1-6 can significantly control the inflammatory factor IFN-γ in chickens.

[0109] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or substitute equivalents for some of the technical features. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A Chinese medicine composition for treating salpingitis in laying hens, characterized in that: The traditional Chinese medicine composition consists of the following components in parts by weight: 25-35 parts of dandelion, 15-25 parts of motherwort, 10-20 parts of plantain seeds, 10-20 parts of forsythia, 10-20 parts of salvia miltiorrhiza, and 5-15 parts of atractylodes macrocephala.

2. A Chinese medicine composition for treating salpingitis in laying hens according to claim 1, characterized in that: The traditional Chinese medicine composition consists of the following components in parts by weight: 25 parts of dandelion, 22 parts of motherwort, 15 parts of plantain seeds, 18 parts of forsythia, 14 parts of salvia miltiorrhiza, and 12 parts of atractylodes.

3. A Chinese medicine composition for treating salpingitis in laying hens according to claim 1, characterized in that: The traditional Chinese medicine composition consists of the following components in parts by weight: 35 parts of dandelion, 25 parts of motherwort, 20 parts of plantain seeds, 10 parts of forsythia, 10 parts of salvia miltiorrhiza, and 15 parts of atractylodes.

4. A Chinese medicine composition for treating salpingitis in laying hens according to claim 1, characterized in that: The traditional Chinese medicine composition consists of the following components in parts by weight: 20 parts of dandelion, 24 parts of motherwort, 15 parts of plantain seeds, 18 parts of forsythia, 15 parts of salvia miltiorrhiza, and 12 parts of atractylodes.

5. A Chinese medicine composition for treating salpingitis in laying hens according to claim 1, characterized in that: The traditional Chinese medicine composition consists of the following components in parts by weight: 30 parts of dandelion, 15 parts of motherwort, 10 parts of plantain seeds, 20 parts of forsythia, 20 parts of salvia miltiorrhiza and 5 parts of atractylodes.

6. A Chinese medicine composition for treating salpingitis in laying hens according to claim 1, characterized in that: The traditional Chinese medicine composition consists of the following components in parts by weight: 26 parts of dandelion, 18 parts of motherwort, 16 parts of plantain seeds, 14 parts of forsythia, 15 parts of salvia miltiorrhiza, and 10 parts of atractylodes.

7. The method for preparing the Chinese medicine composition according to any one of claims 1 to 6, characterized in that: The raw materials of the formula amounts are mixed and boiled in water to obtain a decoction.

8. The method for preparing the Chinese medicine composition according to any one of claims 1 to 6, characterized in that: The raw materials of the formula amount are mixed, dried, and ultrafinely ground to obtain granules.

9. Use of the Chinese medicine composition according to any one of claims 1 to 6 in preparing medicine or feed for treating salpingitis in laying hens.

10. A drug, characterized in that: The active ingredient of the medicine is the traditional Chinese medicine composition according to any one of claims 1 to 6.

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