Method for efficiently cleaning protein of bone tissue

Abstract

The invention belongs to the technical field of bone tissue disinfection treatment, and particularly relates to a method for efficiently cleaning protein of bone tissues, which comprises the following steps: A1, primarily treating the bone tissues, transferring the primarily treated bone tissues into cleaning equipment, and injecting a sodium hydroxide solution; a2, the soaking time is set, the bone tissue is taken out after soaking, and the sodium hydroxide solution is controlled to flow circularly in the soaking process; a3, capturing and filtering solid substances in the sodium hydroxide solution in the circular flowing process of the sodium hydroxide solution; a4, the bone tissue is taken out, alkaline solution residues on the surface of the bone tissue are neutralized, and a water gun is used for flushing the bone tissue. The purity of the solution is maintained through the cooperation of the structure, so that the disinfection efficiency can be maintained and the disinfection effect can be improved when bone tissues are treated.

Description

Technical Field

[0001] The invention belongs to the technical field of bone tissue disinfection, in particular to a method for efficiently cleaning protein from bone tissue. Background Art

[0002] As a highly specialized connective tissue in the human body, bone tissue not only provides mechanical support to the body through the exquisite structure of cortical bone and cancellous bone to ensure motor function, but also participates in key physiological processes such as calcium-phosphorus metabolism and hematopoietic regulation. In the biomedical field, bone tissue-related research is crucial to understanding the mechanisms of bone diseases and developing innovative therapies; in orthopedic clinical practice, the success of fracture repair and bone transplantation surgery depends on the precise grasp of the characteristics of bone tissue. However, the protein layer on the surface of bone tissue, including extracellular matrix components such as collagen and osteopontin, as well as adsorbed plasma proteins and immunomodulatory factors, significantly affects cell-material interactions and immune responses. These proteins can alter the conformation of cell adhesion sites and interfere with the osteogenic differentiation pathway of stem cells. In the context of allogeneic bone transplantation, surface proteins can trigger rejection by the host immune system.

[0003] In the prior art, it is common to immerse bone tissue in a low-concentration alkaline solution to disinfect the bone tissue and remove residual protein on the surface. Since the solution is alkaline and corrosive, it is usually immersed in a static state, which leads to a low solution concentration at the contact position of the bone tissue, resulting in uneven solution concentration and affecting the quality of the operation.

[0004] To this end, the present invention provides a method for efficiently cleaning protein from bone tissue. Summary of the Invention

[0005] In order to make up for the deficiencies of the prior art, at least one technical problem raised in the background technology is solved.

[0006] The technical solution adopted by the present invention to solve the technical problem is: the method for efficiently cleaning protein from bone tissue of the present invention comprises the following steps: A1. Preliminary treatment of bone tissue: transfer the preliminarily treated bone tissue to a cleaning device and inject sodium hydroxide solution; A2. Set the soaking time, remove the bone tissue after soaking, and control the circulation of the sodium hydroxide solution during the soaking process; A3. Capture and filter solid matter in the sodium hydroxide solution during its circulation process; A4. Remove the bone tissue and neutralize the alkaline solution residue on the surface of the bone tissue. Use a water gun to rinse the bone tissue.

[0007] Preferably, in step A1, the step of preliminarily processing the bone tissue includes: B1. Remove the target bone tissue and place it in sterile saline to prepare for subsequent cleaning; B2. Place the bone tissue in a container and rinse repeatedly with saline; B3. Use a knife to cut the bone tissue into small, uniform pieces to increase the contact area of the cleaning solution; B4. Disinfect the cleaned bone tissue.

[0008] Preferably, the cleaning device in step B1 includes a storage unit, a control unit, a filtering mechanism, and a scraping mechanism; The storage portion includes a storage box, a closing cover, and a storage bucket. The closing cover is rotatably mounted on the outer wall of the storage box. The storage bucket is used to accommodate the bone tissue preliminarily processed in step A1. The control part includes a liquid phase pipe, a gas phase pipe and a control pump. The control pump extracts the solution from the inner cavity of the storage box through the liquid phase pipe and discharges it through the gas phase pipe.

[0009] The filtering mechanism includes a carrier frame and a filter hopper. The filter hopper is mounted on the inner wall of the storage box through the carrier frame and is located in the forward direction of the gas phase pipe. The scraping mechanism is used to scrape the bottom of the filter funnel.

[0010] Preferably, the scraping mechanism includes a mounting frame, a guide plate and a sliding plate. The mounting frame is slidably mounted on the upper end of the filter bucket, the guide plate is fixedly mounted on the bottom surface of the mounting frame, the outer wall of the sliding plate slides in contact with the inner wall of the guide plate, and the outer wall of the sliding plate slides in contact with the inner wall of the filter bucket.

[0011] Preferably, a guide strip is fixedly mounted on the outer wall of the filter bucket, and a guide block is fixedly mounted on the outer wall of the mounting frame, and the outer wall of the guide block fits with the inner wall of the guide strip.

[0012] Preferably, the outer wall of the guide block is made of elastic material; A control cylinder is fixedly mounted on the inner wall of the mounting frame, a control plug is slidably mounted on the inner cavity of the control cylinder, and a transmission collar is fixedly mounted on the outer wall of the control plug via a connecting rod; A driving motor is fixedly installed on the outer wall of the storage box, and a threaded rod matched with a transmission collar is fixedly installed on the output shaft of the driving motor.

[0013] Preferably, a mounting cylinder connected to the inner cavity of the control cylinder is fixedly installed on the outer wall of the guide plate, a mounting plug is fixedly installed on the inner wall of the mounting cylinder, a connecting rod is fixedly installed on the bottom of the mounting plug, and the other end of the connecting rod is fixedly connected to the outer wall of the sliding plate.

[0014] Preferably, a connecting frame is fixedly installed on the outer wall of the mounting frame, a supporting plate is fixedly installed on the bottom surface of the connecting frame, the supporting plate is located at the bottom of the filter bucket, and a recoil nozzle is fixedly installed on the upper end surface. There are multiple recoil nozzles, and the multiple recoil nozzles are evenly arranged along the outer wall of the supporting plate.

[0015] Preferably, a control box is fixedly mounted on the bottom surface of the carrier frame, a sealing plate is elastically mounted on the inner wall of the control box, an air inlet pipe and an exhaust pipe are fixedly mounted on the outer wall of the control box, and the exhaust pipe is connected to the recoil nozzle; The inner wall of the control box is rotatably mounted with a transmission shaft via a torsion spring, and the outer wall of the transmission shaft is fixedly mounted with a cam for pressing the sealing plate; A traction rope is wound around the outer wall of the transmission shaft, a transmission frame is fixedly installed on the outer wall of the transmission collar, and one end of the traction rope is fixedly connected to the transmission frame.

[0016] Preferably, a blocking plate is rotatably mounted on the inner wall of the control box via a torsion spring, and the blocking plate is used to block the exhaust pipe; A top pressure plate is fixedly installed on the outer wall of the sealing plate, and the outer walls of the top pressure plate and the blocking plate are both inclined.

[0017] The beneficial effects of the present invention are as follows: 1. The present invention sets a liquid phase pipe and a gas phase pipe. When the pump is controlled to run in the forward direction, the solution in the storage box is extracted and then discharged through the gas phase pipe, thereby controlling the circulation of the sodium hydroxide solution to maintain the uniform concentration of internal sodium hydroxide, thereby maintaining the efficiency of disinfection and protein removal. When the pump is controlled to rotate in the reverse direction, the air inside the storage box is extracted and discharged through the liquid phase pipe at the bottom of the storage box, thereby achieving internal aeration and disturbing the flow of the internal solution, thereby utilizing the flow of water to improve the efficiency of residual protein separation. A filter bucket is also set. When the internal solution circulates, the solution discharged from the gas phase pipe contains a large amount of residue. At this time, it is filtered and collected through the filter bucket, thereby maintaining the purity of the solution. Furthermore, when treating bone tissue, the efficiency of disinfection can be maintained and a disinfection effect can be provided.

[0018] 2. The present invention is provided with a guide plate and a sliding plate. When the mounting frame slides forward, the sliding plate slides downward, that is, the bottom surface is in contact with the bottom surface of the inner cavity of the filter bucket. At this time, the filter bucket is scraped, and as the sliding plate and the mounting frame slide horizontally synchronously, the internal residue is pushed to one side of the filter bucket. When the mounting frame slides reversely, the sliding plate slides upward until the bottom of the sliding plate is separated from the bottom surface of the inner cavity of the filter bucket, until the mounting frame is reset. Through the reciprocating sliding of the mounting frame in the horizontal direction and the reciprocating sliding of the sliding plate in the vertical direction, the residue inside the filter bucket can be pushed to one side of the filter bucket, thereby maintaining the permeability of the scraped area of the bottom of the filter bucket. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The present invention will be further described below with reference to the accompanying drawings.

[0020] Figure 1 It is a schematic structural diagram of the present invention as a whole; Figure 2 It is a schematic diagram of the internal structure of the storage box of the present invention; Figure 3 This is a schematic diagram of the installation of the filter bucket in the present invention; Figure 4 It is a transmission schematic diagram of the transmission collar in the present invention; Figure 5 It is a schematic diagram of the installation of the sliding plate in the present invention; Figure 6 This is a schematic diagram of the internal structure of the control cylinder in the present invention; Figure 7 It is a structural diagram of the control box in the present invention; Figure 8 is a cross-sectional view of the control box of the present invention; Figure 9 This is a schematic diagram of the installation of the blocking plate in the present invention; Figure 10 and 11 It is a flow chart of the protein cleaning method of the present invention.

[0021] In the figure: 1. Storage box; 2. Control pump; 3. Closing cover; 4. Gas phase pipe; 5. Drive motor; 6. Carrying frame; 7. Storage bucket; 8. Threaded rod; 9. Filter bucket; 10. Liquid phase pipe; 11. Drive shaft; 12. Drive collar; 13. Guide strip; 14. Drive frame; 15. Connecting frame; 16. Traction rope; 17. Control box; 18. Sliding plate; 19. Control cylinder; 20. Guide block; 21. Mounting frame; 22. Guide plate; 23. Carrying plate; 24. Recoil nozzle; 25. Mounting cylinder; 26. Control plug; 27. Sealing plate; 28. Connecting rod; 29. Mounting plug; 30. Inlet pipe; 31. Exhaust pipe; 32. Blocking plate; 33. Top pressure plate; 34. Cam. DETAILED DESCRIPTION

[0022] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific implementation methods.

[0023] like Figure 1-2 As shown, the method for efficiently cleaning protein from bone tissue of the present invention comprises the following steps: A1. Preliminary treatment of bone tissue: transfer the preliminarily treated bone tissue to a cleaning device and inject sodium hydroxide solution; A2. Set the soaking time, remove the bone tissue after soaking, and control the circulation of the sodium hydroxide solution during the soaking process; A3. Capture and filter solid matter in the sodium hydroxide solution during its circulation process; A4. Remove the bone tissue and neutralize the alkaline solution residue on the surface of the bone tissue. Use a water gun to rinse the bone tissue.

[0024] In step A1, the steps of preliminary processing of bone tissue include: B1. Remove the target bone tissue and place it in sterile saline to prepare for subsequent cleaning; B2. Place the bone tissue in a container and rinse repeatedly with saline; B3. Use a knife to cut the bone tissue into small, uniform pieces to increase the contact area of the cleaning solution; B4. Disinfect the cleaned bone tissue.

[0025] like Figure 3-11 As shown, the cleaning device in step B1 includes a storage unit, a control unit, a filtering mechanism, and a scraping mechanism; The storage portion includes a storage box 1, a closing cover 3 and a storage bucket 7. The closing cover 3 is rotatably arranged on the outer wall of the storage box 1, wherein the closing cover 3 is connected to the storage box 1 through a hinge, and a sealing gasket is provided at the contact position. The storage box 1 is used for sodium hydroxide solution, and the storage bucket 7 is used to accommodate the bone tissue preliminarily processed in step A1. The storage bucket 7 has a mesh structure.

[0026] After the bone tissue is preliminarily processed, sodium hydroxide solution is injected into the storage box 1 (in order to minimize damage to soft tissue, the concentration of the sodium hydroxide solution in this embodiment does not exceed 10%), the bone tissue is placed in the storage bucket 7, and then the storage bucket 7 is placed in the storage box 1 until the bone tissue is immersed in the sodium hydroxide solution. The sodium hydroxide solution is used to disinfect bacteria and viruses on the surface of the bone tissue, and the alkaline solution is used to clean the residual protein on the surface of the bone tissue.

[0027] The control unit includes a liquid phase pipe 10, a gas phase pipe 4 and a control pump 2. The control pump 2 extracts the solution from the inner cavity of the storage box 1 through the liquid phase pipe 10 and discharges it through the gas phase pipe 4. The liquid phase pipe 10 is fixed to the outer wall of the storage box 1 and is located below the liquid level of the sodium hydroxide solution. The gas phase pipe 4 is fixed to the closing cover 3. After the closing cover 3 is closed, the gas phase pipe 4 is located above the liquid level of the sodium hydroxide solution.

[0028] The control pump 2 uses a common diaphragm pump, and the liquid phase pipe 10 and the gas phase pipe 4 are respectively connected to the two ports (input end and output end) of the control pump 2. When the control pump 2 is running in the forward direction, the solution in the storage tank 1 is extracted and then discharged through the gas phase pipe 4, thereby controlling the circulation of the sodium hydroxide solution to maintain the uniform concentration of the internal sodium hydroxide, thereby maintaining the efficiency of disinfection and protein removal.

[0029] When the pump 2 is controlled to rotate in the reverse direction, the air inside the storage box 1 is extracted and discharged from the liquid phase pipe 10 at the bottom of the storage box 1, thereby achieving internal aeration and disturbing the flow of the internal solution, thereby utilizing the flow of water to improve the efficiency of residual protein separation.

[0030] The filtering mechanism includes a supporting frame 6 and a filter bucket 9. The filter bucket 9 is installed on the inner wall of the storage box 1 through the supporting frame 6. The filter bucket 9 is located in the positive direction of the gas phase tube 4. When the internal solution circulates, the solution discharged from the gas phase tube 4 contains a large amount of residue (such as meat residue fallen off the epidermis of bone tissue). At this time, it is filtered and collected by the filter bucket 9, thereby maintaining the purity of the solution, and thus maintaining the efficiency of disinfection when processing bone tissue.

[0031] In order to prevent the bottom of the filter bucket 9 from being blocked, a scraping mechanism is provided. The scraping mechanism is used to scrape the bottom of the filter bucket 9. When the gas phase pipe 4 stops discharging the solution, the bottom of the filter bucket 9 is scraped, thereby preventing the permeability of the filter bucket 9 and maintaining the filtering efficiency of the residue.

[0032] The scraping mechanism includes a mounting frame 21 , a guide plate 22 and a sliding plate 18 . The mounting frame 21 is slidably mounted on the upper end of the filter bucket 9 , wherein the mounting frame 21 can slide back and forth along the interior of the filter bucket 9 .

[0033] The guide plate 22 is fixedly mounted on the bottom surface of the mounting frame 21, the outer wall of the sliding plate 18 slides in contact with the inner wall of the guide plate 22, and the outer wall of the sliding plate 18 slides in contact with the inner wall of the filter bucket 9. The sliding of the mounting frame 21 drives the guide plate 22 and the sliding plate 18 to slide synchronously. The sliding plate 18 is used to scrape the bottom of the filter bucket 9 to achieve scraping and cleaning of the filter bucket 9.

[0034] When the mounting frame 21 slides forward, the sliding plate 18 slides downward, that is, the bottom surface is in contact with the bottom surface of the inner cavity of the filter bucket 9. At this time, the filter bucket 9 is scraped, and as the sliding plate 18 and the mounting frame 21 slide horizontally synchronously, the internal residue is pushed to one side of the filter bucket 9.

[0035] When the mounting bracket 21 slides in the reverse direction, the sliding plate 18 slides upward until the bottom of the sliding plate 18 is separated from the bottom surface of the inner cavity of the filter bucket 9, and until the mounting bracket 21 is reset.

[0036] By the reciprocating sliding of the mounting frame 21 in the horizontal direction and the reciprocating sliding of the sliding plate 18 in the vertical direction, the residue inside the filter bucket 9 can be pushed to one side of the filter bucket 9, thereby maintaining the permeability of the scraped area at the bottom of the filter bucket 9.

[0037] In addition, when there is too much residue inside the filter bucket 9, the sliding plate 18 stops after scraping, and at this time maintains the state of squeezing the residue. A frame structure is formed through the sliding plate 18 and the interior of one side of the filter bucket 9 to store the residue while maintaining the filtering effect of the filter bucket 9 on the other side of the sliding plate 18.

[0038] As a preferred embodiment of the present invention, a guide bar 13 is fixedly installed on the outer wall of the filter bucket 9, and a guide block 20 is fixedly installed on the outer wall of the mounting frame 21. The outer wall of the guide block 20 is fitted with the inner wall of the guide bar 13, and the guide block 20 and the inner wall of the guide bar 13 are slidably fitted, thereby maintaining the sliding stability of the mounting frame 21 when the mounting frame 21 slides.

[0039] The outer wall of the guide block 20 is made of elastic material. The outer wall of the guide block 20 is made of rubber material to increase the sliding friction between the guide block 20 and the inner wall of the guide strip 13.

[0040] The control cylinder 19 is fixedly installed on the inner wall of the mounting frame 21, and the control plug 26 is slidably installed in the inner cavity of the control cylinder 19. The outer wall of the control plug 26 is sealed and fitted with the inner wall of the control cylinder 19. The control plug 26 is pressed and pulled to adjust the internal air pressure of the control cylinder 19 until the mounting frame 21 slides synchronously. The friction between the control plug 26 and the control cylinder 19 is not higher than the friction between the guide block 20 and the guide bar 13.

[0041] A transmission collar 12 is fixedly mounted on the outer wall of the control plug 26 via a connecting rod. The transmission collar 12 is an internally threaded ring.

[0042] The outer wall of the storage box 1 is fixedly mounted with a drive motor 5, and the output shaft of the drive motor 5 is fixedly mounted with a threaded rod 8 that cooperates with the transmission collar 12. The drive motor 5 is a servo motor used to control the rotation of the threaded rod 8, thereby controlling the movement of the transmission collar 12.

[0043] When the threaded rod 8 controls the transmission sleeve 12 to move forward, the control plug 26 slides forward. At this time, the air pressure inside the control cylinder 19 increases until the control cylinder 19 and the control plug 26 slide synchronously, and drive the mounting frame 21 to slide, thereby scraping the filter bucket 9.

[0044] When the threaded rod 8 rotates in the reverse direction, the control plug 26 slides in the reverse direction. At this time, the air pressure in the inner cavity of the control cylinder 19 decreases until the control cylinder 19 and the control plug 26 slide synchronously.

[0045] The outer wall of the guide plate 22 is fixedly mounted with a mounting cylinder 25 which is connected to the inner cavity of the control cylinder 19. The inner wall of the mounting cylinder 25 is fixedly mounted with a mounting plug 29. The outer wall of the mounting plug 29 is sealed and fits with the inner wall of the mounting cylinder 25. At the same time, the air pressure in the inner cavity of the mounting cylinder 25 changes synchronously with the air pressure in the inner cavity of the control cylinder 19, which is used to control the sliding of the mounting plug 29.

[0046] A connecting rod 28 is fixedly installed at the bottom of the mounting plug 29, and the other end of the connecting rod 28 is fixedly connected to the outer wall of the sliding plate 18. The sliding of the mounting plug 29 drives the sliding plate 18 to rise and fall, thereby controlling the synchronous lateral sliding of the mounting frame 21 and the vertical sliding of the sliding plate 18.

[0047] As a preferred embodiment of the present invention, a connecting frame 15 is fixedly mounted on the outer wall of the mounting frame 21 , and when the mounting frame 21 slides, the connecting frame 15 is driven to slide synchronously.

[0048] A supporting plate 23 is fixedly installed on the bottom surface of the connecting frame 15. The supporting plate 23 is located at the bottom of the filter bucket 9, and a backflush nozzle 24 is fixedly installed on the upper end surface. By jetting through the backflush nozzle 24, the residue blocked in the mesh at the bottom of the filter bucket 9 can be cleaned as much as possible, further improving the filtration efficiency of the filter bucket 9, thereby maintaining the disinfection effect.

[0049] There are multiple backflushing nozzles 24, which are evenly arranged along the outer wall of the supporting plate 23 to increase the backflushing area and improve the cleaning effect. The backflushing nozzles 24 move synchronously with the mounting frame 21 and can backflush the filter bucket 9 after scraping.

[0050] The control box 17 is fixedly mounted on the bottom surface of the carrier 6 , and a sealing plate 27 is elastically mounted on the inner wall of the control box 17 . The outer wall of the sealing plate 27 is in sealing contact with the inner wall of the control box 17 .

[0051] An air intake pipe 30 and an exhaust pipe 31 are fixedly installed on the outer wall of the control box 17. The exhaust pipe 31 is connected to the recoil nozzle 24. A one-way valve is provided inside the air intake pipe 30 and the exhaust pipe 31, and the conduction directions are opposite. The air intake pipe 30 is controlled by the reciprocating sliding sealing plate 27 to intake air into the control box 17, and the exhaust pipe 31 is controlled to exhaust air to the recoil nozzle 24, serving as the air source for recoil of the recoil nozzle 24.

[0052] The inner wall of the control box 17 is rotatably mounted with a transmission shaft 11 through a torsion spring, and the outer wall of the transmission shaft 11 is fixedly mounted with a cam 34 for pressing the sealing plate 27. After the transmission shaft 11 is rotated, the elastic force of the torsion spring is used to control the transmission shaft 11 to reset. During the rotation of the transmission shaft 11, the cam 34 rotates synchronously, cooperating with the elastic force exerted on the sealing plate 27 to control the reciprocating sliding of the sealing plate 27.

[0053] A traction rope 16 is wound around the outer wall of the transmission shaft 11, and a transmission frame 14 is fixedly installed on the outer wall of the transmission collar 12. One end of the traction rope 16 is fixedly connected to the transmission frame 14. When the transmission collar 12 slides forward, the traction rope 16 pulls the transmission shaft 11 to rotate. When the transmission collar 12 slides reversely, the torsion spring controls the transmission shaft 11 to rotate reversely. At this time, the transmission shaft 11 synchronously reels the traction rope 16 for reuse.

[0054] A blocking plate 32 is rotatably mounted on the inner wall of the control box 17 via a torsion spring. The blocking plate 32 is used to block the exhaust pipe 31 , and the outer wall of the blocking plate 32 has a tendency to stick to the end of the exhaust pipe 31 .

[0055] A top pressure plate 33 is fixedly installed on the outer wall of the sealing plate 27. The outer walls of the top pressure plate 33 and the blocking plate 32 are both inclined. When the blocking plate 32 closes the exhaust pipe 31, the sealing plate 27 slides to compress the air inside the control box 17 until the top pressure plate 33 slides to lift the blocking plate 32, and then discharges the compressed air to the recoil nozzle 24 to increase the flow rate of the airflow during recoil.

[0056] The above-mentioned front, back, left, right, up and down are all based on the Figure 1 As a benchmark, according to the person's observation perspective, the side of the device facing the observer is defined as the front, the left side of the observer is defined as the left, and so on.

[0057] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the scope of protection of the present invention.

[0058] The basic principles, main features, and advantages of the present invention are shown and described above. Those skilled in the art should understand that the present invention is not limited to the foregoing embodiments. The foregoing embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and modifications may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and modifications are intended to fall within the scope of the present invention. The scope of protection claimed in the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for efficiently cleaning protein from bone tissue, characterized by: The following steps are involved: A1. Preliminary treatment of bone tissue: transfer the preliminarily treated bone tissue to a cleaning device and inject sodium hydroxide solution; A2. Set the soaking time, remove the bone tissue after soaking, and control the circulation of the sodium hydroxide solution during the soaking process; A3. Capture and filter solid matter in the sodium hydroxide solution during its circulation process; A4. Remove the bone tissue and neutralize the alkaline solution residue on the surface of the bone tissue. Use a water gun to rinse the bone tissue.

2. The method for efficiently cleaning protein from bone tissue according to claim 1, wherein: In step A1, the steps of preliminary processing of bone tissue include: B1. Remove the target bone tissue and place it in sterile saline to prepare for subsequent cleaning; B2. Place the bone tissue in a container and rinse repeatedly with saline; B3. Use a knife to cut the bone tissue into small, uniform pieces to increase the contact area of the cleaning solution; B4. Disinfect the cleaned bone tissue.

3. The method for efficiently cleaning protein from bone tissue according to claim 1, wherein: The cleaning device in step A1 includes a storage unit, a control unit, a filtering mechanism, and a scraping mechanism; The storage portion comprises a storage box (1), a closing cover (3) and a storage bucket (7), wherein the closing cover (3) is rotatably arranged on the outer wall of the storage box (1), and the storage bucket (7) is used to accommodate the bone tissue preliminarily processed in step A1; The control unit comprises a liquid phase pipe (10), a gas phase pipe (4) and a control pump (2), wherein the control pump (2) extracts the solution from the inner cavity of the storage box (1) through the liquid phase pipe (10) and discharges the solution through the gas phase pipe (4); The filtering mechanism comprises a carrier frame (6) and a filter hopper (9), wherein the filter hopper (9) is mounted on the inner wall of the storage box (1) via the carrier frame (6), and the filter hopper (9) is located in the forward direction of the gas phase pipe (4); The scraping mechanism is used to scrape the bottom of the filter hopper (9).

4. The method for efficiently cleaning protein from bone tissue according to claim 3, wherein: The scraping mechanism comprises a mounting frame (21), a guide plate (22) and a sliding plate (18), wherein the mounting frame (21) is slidably mounted on the upper end of the filter bucket (9), and the guide plate (22) is fixedly mounted on the bottom surface of the mounting frame (21), and the outer wall of the sliding plate (18) is slidably fitted with the inner wall of the guide plate (22), and the outer wall of the sliding plate (18) is slidably fitted with the inner wall of the filter bucket (9).

5. The method for efficiently cleaning protein from bone tissue according to claim 4, wherein: A guide strip (13) is fixedly mounted on the outer wall of the filter hopper (9), and a guide block (20) is fixedly mounted on the outer wall of the mounting frame (21), wherein the outer wall of the guide block (20) is in contact with the inner wall of the guide strip (13).

6. The method for efficiently cleaning protein from bone tissue according to claim 5, wherein: The outer wall of the guide block (20) is made of elastic material; A control cylinder (19) is fixedly mounted on the inner wall of the mounting frame (21), a control plug (26) is slidably mounted in the inner cavity of the control cylinder (19), and a transmission collar (12) is fixedly mounted on the outer wall of the control plug (26) via a connecting rod; A drive motor (5) is fixedly mounted on the outer wall of the storage box (1), and a threaded rod (8) that cooperates with a transmission collar (12) is fixedly mounted on the output shaft of the drive motor (5).

7. The method for efficiently cleaning protein from bone tissue according to claim 6, wherein: A mounting cylinder (25) communicating with the inner cavity of the control cylinder (19) is fixedly mounted on the outer wall of the guide plate (22), a mounting plug (29) is fixedly mounted on the inner wall of the mounting cylinder (25), a connecting rod (28) is fixedly mounted on the bottom of the mounting plug (29), and the other end of the connecting rod (28) is fixedly connected to the outer wall of the sliding plate (18).

8. The method for efficiently cleaning protein from bone tissue according to claim 7, wherein: A connecting frame (15) is fixedly mounted on the outer wall of the mounting frame (21), a supporting plate (23) is fixedly mounted on the bottom surface of the connecting frame (15), the supporting plate (23) is located at the bottom of the filter bucket (9), and a recoil nozzle (24) is fixedly mounted on the upper end surface, a plurality of recoil nozzles (24) are provided, and the plurality of recoil nozzles (24) are evenly arranged along the outer wall of the supporting plate (23).

9. The method for efficiently cleaning protein from bone tissue according to claim 8, wherein: A control box (17) is fixedly mounted on the bottom surface of the carrier (6), a sealing plate (27) is elastically mounted on the inner wall of the control box (17), an air intake pipe (30) and an exhaust pipe (31) are fixedly mounted on the outer wall of the control box (17), and the exhaust pipe (31) is connected to the recoil nozzle (24); A transmission shaft (11) is rotatably mounted on the inner wall of the control box (17) via a torsion spring, and a cam (34) for pressing the sealing plate (27) is fixedly mounted on the outer wall of the transmission shaft (11); A traction rope (16) is wound around the outer wall of the transmission shaft (11), a transmission frame (14) is fixedly mounted on the outer wall of the transmission collar (12), and one end of the traction rope (16) is fixedly connected to the transmission frame (14).

10. The method for efficiently cleaning protein from bone tissue according to claim 9, characterized in that: A blocking plate (32) is rotatably mounted on the inner wall of the control box (17) via a torsion spring, and the blocking plate (32) is used to block the exhaust pipe (31); A top pressure plate (33) is fixedly mounted on the outer wall of the sealing plate (27), and the outer walls of the top pressure plate (33) and the blocking plate (32) are both inclined.

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