Variable-frequency plate cleaning device for ELISA (enzyme-linked immunosorbent assay) plate

Abstract

The invention discloses a variable-frequency plate cleaning device for ELISA (enzyme-linked immunosorbent assay) elisa plates, which belongs to the field of medical instruments and comprises a plate cleaning air bellow for providing moving airflow, a plurality of conical air pipe arrays arranged at the operation end of the plate cleaning air bellow, and the plate cleaning air bellow is communicated with the conical air pipes; the fixed cabin is rotationally connected with the clean plate air box, a limiting piece is movably arranged in the fixed cabin, when the limiting piece moves to a limiting position, the relative position relation between the fixed cabin and the ELISA plate in the fixed cabin is locked, and when the fixed cabin rotates to a working position, the conical air pipes stretch into the ELISA holes in a one-to-one correspondence mode; the oscillator is connected to the operation end part, the vibration frequency of the oscillator is smaller than 10000 Hz, the amplitude of the oscillator is not smaller than 0.5 mm, the oscillator overcomes the infiltration force between liquid drops and the enzyme label holes when vibrating, and when airflow enters the enzyme label holes in an accelerated mode from the clean plate air box through the conical air pipe, the liquid drops are driven to be separated from the enzyme label holes to move towards the operation end part. Through the arrangement, the enzyme label plate cleaning device can quickly and efficiently complete the enzyme label plate cleaning operation.

Description

Technical Field

[0001] The invention belongs to the technical field of medical devices, and particularly relates to a frequency conversion and plate cleaning device for an ELISA enzyme labeling plate. Background Art

[0002] ELISA (Enzyme-Linked Immunosorbent Assay) is an immunological method used to detect and quantify proteins, antibodies, and antigens, and is widely used in biomedical research and diagnostics. The basic ELISA protocol includes coating, washing, blocking, sample loading, washing, adding detection antibodies, washing, color development, termination, and detection. Before and after the experiment, the plate must be washed multiple times and the liquid in the wells must be dried to remove any interference from excess sample and unbound antibodies.

[0003] Currently, this step is mainly performed manually by patting dry on the operating table with absorbent paper. However, this method poses biosafety risks and cannot cope with a large amount of clinical work. In addition, the quality of the pat is greatly affected by human factors, and the accuracy cannot be controlled.

[0004] Therefore, an auxiliary device for quickly and efficiently completing the ELISA plate cleaning operation is designed. The auxiliary device is specifically an ELISA plate frequency conversion cleaning device. Summary of the Invention

[0005] In order to overcome the problems raised in the background technology, the present invention adopts the following technical solutions:

[0006] An ELISA plate frequency conversion cleaning device comprises: a cleaning bellows, which provides a moving airflow; a plurality of conical air duct arrays are arranged at the operating end of the cleaning bellows, and the cleaning bellows is connected to the conical air ducts; a fixed cabin, which is rotatably connected to the cleaning bellows; a limit member is provided for movement in the fixed cabin; when the limit member moves to a limit position, the relative position relationship between the fixed cabin and the ELISA plate in the fixed cabin is locked; when the fixed cabin rotates to a working position, the conical air ducts extend into the ELISA wells one by one; an oscillator, which is connected to the operating end; the vibration frequency of the oscillator is less than 10000Hz and the amplitude is not less than 0.5mm; when the oscillator vibrates, it overcomes the wetting force between the droplets and the ELISA wells; when the airflow accelerates from the cleaning bellows into the ELISA wells through the conical air ducts, it drives the droplets to separate from the ELISA wells and move toward the operating end.

[0007] Furthermore, the fixed cabin has a placement groove, the movement of the limiting part is arranged in the placement groove, an elastic part is arranged between the limiting part and the placement groove, and the limiting part presses the ELISA plate against the inner wall of the fixed cabin under the drive of the elastic part.

[0008] Furthermore, the limiting member includes a push plate, the height of which in the placement groove is greater than the thickness of the ELISA plate, and the end of the push plate facing the ELISA plate has an inclined surface facing the bottom of the placement groove. When the limiting member is pressed against the ELISA plate, the push plate provides a component force to press the ELISA plate toward the bottom of the placement groove.

[0009] Furthermore, the fixed cabin includes a cabin body and a connecting basket that are rotatably connected, the connecting basket is rotatably connected to the operating end, and the cabin body has a contact end. When the fixed cabin moves to the working position, the contact end contacts the operating end.

[0010] Furthermore, a gap is provided between the outer wall of the cabin and the placement groove. The depth of the placement groove is greater than the thickness of the ELISA plate. When the ELISA plate is fixed in the placement groove, the outer wall of the ELISA plate can be exposed to the outside through the gap.

[0011] Furthermore, the operating end has a boss surrounding the outside of the conical air duct, and the area of the outer contour of the boss is not less than the area of the contact end; the connecting basket includes adapter plates symmetrically arranged on both sides of the cabin body, and when the fixed cabin moves to the working position, the contact end contacts the boss, and the adapter plates block the gap on both sides of the cabin body.

[0012] Furthermore, the connecting basket further comprises a blocking piece connected to the adapter piece, and when the cabin body contacts the boss, the blocking piece is pressed against an end portion of the cabin body away from the contact end portion.

[0013] Furthermore, the operating end is provided with a water diversion groove, which is evenly distributed at the operating end and divides the conical air duct into several groups; the operating end is also provided with a water collection groove, and the water diversion grooves are all connected to the water collection groove, and the droplets enter the water diversion groove and move towards the water collection groove.

[0014] Furthermore, the clean plate bellows is provided with a drainage channel which is connected to the water collecting trough. When the fixed cabin is in the working position, the airflow ejected from the conical air duct drives the droplets in the enzyme labeling holes to slide along the outer wall of the conical air duct toward the operating end and into the water diversion trough.

[0015] Furthermore, the outer wall of the clean plate bellows is connected with a drain pipe, which is communicated with the drainage channel.

[0016] Furthermore, the movement of the limiting member portion is arranged in the placement groove, and the outer wall of the limiting member is provided with a thread. When the limiting member rotates around its own axis, the length of the portion located in the placement groove changes.

[0017] Furthermore, it also includes a shock-absorbing pad and a bottom bracket, wherein the shock-absorbing pad is connected between the clean plate bellows and the bottom bracket, and the shock-absorbing pad reduces the oscillating movement of the bottom bracket when the oscillator is working.

[0018] Furthermore, an air flow channel is provided in the clean plate air box, and the air flow channel is connected to the air inlet provided on the outer wall of the clean plate air box. A fan is provided in the air flow channel; when the fan is working, the external air flow enters the clean plate air box from the air inlet and is compressed at the conical air duct and then ejected out at an accelerated speed.

[0019] Beneficial effects of the present invention:

[0020] 1. The present invention is equipped with an oscillator and a tapered air duct connected to a plate cleaning bellows. The oscillator 6 provides low- to medium-frequency continuous vibration with a frequency of less than 10,000 Hz and an amplitude of 1 mm. This frequency of vibration can overcome the wetting force between droplets and the enzyme-labeled wells, effectively shaking droplets of different volumes away from the wells without affecting the antigen-antibody complex bound within the wells. Droplets shaken away from the inner walls of the wells are only affected by gravity, while the airflow ejected through the tapered air duct continuously sweeps across the inner walls of the wells, quickly expelling droplets from each well. This significantly improves the accuracy and efficiency of plate cleaning operations and can significantly increase the effective plate cleaning speed in a large number of clinical plate cleaning operations.

[0021] 2. The present invention is provided with a fixed cabin rotatably connected to the plate cleaning bellows, which can not only adjust the direction of the cabin to different directions for quick disassembly and assembly of enzyme specimens, but also form an operating space between the cabin and the operating end to prevent droplets from splashing when the conical air duct sprays air into the enzyme labeling hole, thereby efficiently cleaning the plate while preventing droplets from escaping to the external environment, and having higher safety. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. Those skilled in the art can also derive other drawings based on these drawings without inventive effort. Among them:

[0023] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0024] Figure 2 This is a schematic diagram of an explosion structure of the present invention;

[0025] Figure 3 A schematic diagram of an assembly structure of the present invention when the limiting member moves to the limiting position;

[0026] Figure 4 For fixed cabin Figure 3 A schematic diagram of an assembly structure of the present invention when the position moves toward the working position;

[0027] Figure 5It is a schematic diagram of an assembly structure of the present invention when the ELISA plate moves to the working position;

[0028] Figure 6 for Figure 5 A schematic diagram of a cross-sectional structure;

[0029] Figure 7 for Figure 6 A schematic diagram of a local structure at A in the middle;

[0030] Figure 8 for Figure 6 A is a schematic diagram of a local structure from another perspective;

[0031] Figure 9 It is a schematic diagram of the overall structure of a clean plate bellows;

[0032] Figure 10 for Figure 9 A schematic diagram of a cross-sectional structure;

[0033] In the figure, 1. Clean plate bellows; 11. Conical air duct; 12. Operating end; 121. Boss; 122. Water diversion trough; 123. Water collecting trough; 13. Drainage channel; 14. Drain pipe; 15. Air flow channel; 151. Air inlet; 152. Fan; 2. Fixed cabin; 21. Limiting member; 211. Push plate; 22. Elastic member; 23. Cabin body; 231. Placement slot; 232. Contact end; 233. Notch; 24. Connecting basket; 241. Adapter; 242. Baffle; 3. ELISA plate; 31. ELISA hole; 4. Oscillator; 5. Shock-absorbing pad; 51. Power switch; 52. Power interface; 6. Bottom bracket. DETAILED DESCRIPTION

[0034] The following is a clear and complete description of the technical solutions in the embodiments of the present invention through specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation methods. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0035] ELISA plate 3 frequency conversion clean plate device, such as Figure 1-10As shown, it includes: a clean plate bellows 1, which provides a moving airflow, and a plurality of conical air ducts 11 are arranged in an array at the operating end 12 of the clean plate bellows 1, and the clean plate bellows 1 is connected to the conical air duct 11; a fixed cabin 2, which is rotatably connected to the clean plate bellows 1, and a limiting member 21 is provided for movement in the fixed cabin 2. When the limiting member 21 moves to a limiting position, the relative position relationship between the fixed cabin 2 and the ELISA plate 3 in the fixed cabin 2 is locked. When the fixed cabin 2 rotates to a working position, the conical air ducts 11 extend into the ELISA holes 31 one by one, thereby ensuring the cleaning effect of a single ELISA hole 31, so that the droplets in each ELISA hole 31 are effectively removed; an oscillator 4, which is connected to the operating end 12, and the vibration frequency of the oscillator 4 is less than 10000 Hz and the amplitude is not less than 0.5 mm. When the oscillator 4 vibrates, it overcomes the wetting force between the droplets and the ELISA holes 31, so that the droplets in the ELISA holes 31 are driven by gravity and airflow to escape outward. When the airflow is accelerated from the clean plate bellows 1 into the enzyme-labeled hole 31 through the tapered air duct 11, the droplets are driven to leave the enzyme-labeled hole 31 and move toward the operating end 12. In the above process, the tapered air duct 11 can effectively increase the impact force of the airflow on the droplets by reducing the ejection area of the airflow and making the ejected airflow more concentrated. In combination with the oscillator 4 breaking the wetting force of the droplets in the enzyme-labeled hole 31, the droplets can diffuse around the impact point of the airflow at the bottom of the enzyme-labeled hole 31. The diffusion path is as follows: Figure 8 As shown by the middle arrow, the airflow drives the liquid droplets along the way that are not constrained by the wetting force to fall toward the outer wall of the tapered air duct 11 and the water guide groove 122 between the tapered air ducts 11.

[0036] In some embodiments of the present application, Figure 1-10 As shown, the fixed cabin 2 includes a cabin body 23 and a connecting basket 24 that are rotatably connected. The connecting basket 24 is rotatably connected to the operating end 12. The cabin body 23 has a contact end 232. When the fixed cabin 2 moves to the working position, the contact end 232 contacts the operating end 12. In the process of placing the ELISA plate 3 into the cabin body 23 and fixing it by the limiter 21, the ELISA plate 3 is easily pushed by the limiter 21 and inevitably collides with the inner wall of the placement groove 231. Since there are droplets to be cleared in the ELISA hole 31, if the ELISA plate 3 is directly placed into the cabin body 23 with the ELISA hole 31 facing downward, the droplets may flow out of the ELISA plate 3 when the ELISA plate 3 vibrates. Therefore, when installing the ELISA plate 3, first adjust the cabin body 23 to the position as shown in FIG. Figure 1 The contact end 232 and the operating end 12 are oriented in the same direction. In this state, the ELISA plate 3 is placed in the placement groove 231 with the ELISA hole 31 facing upward and fixed by the limiter 21. During this process, the droplets are stably present in the ELISA hole 31 under the action of gravity and infiltration force. Figure 3 、 45, the process is to flip the cabin 23 so that the contact end 232 is gradually turned toward the operating end 12, and at the same time, the angle between the connecting basket 24 and the clean plate bellows 1 is adjusted so that the contact end 232 and the operating end 12 are closed, and finally the enzyme labeling holes 31 are aligned one by one with the tapered air duct 11. A gap 233 is provided between the outer wall of the cabin 23 and the placement groove 231. The depth of the placement groove 231 is greater than the thickness of the enzyme labeling plate 3. When the enzyme labeling plate 3 is fixed in the placement groove 231, the outer wall of the enzyme labeling plate 3 can be exposed to the outside through the gap 233, so that the experimenter can easily remove the enzyme labeling plate 3 from the placement groove 231 or adjust the relative position of the enzyme labeling plate 3 and the placement groove 231 when placing the enzyme labeling plate 3 in the placement groove 231.

[0037] In some embodiments of the present application, Figure 1-10 As shown, the fixed cabin 2 has a placement groove 231, which is used to place the ELISA plate 3 and provides movement space for the limiter 21 and the ELISA plate 3. When the ELISA plate 3 is inside the placement groove 231, the ELISA hole 31 is away from the bottom of the placement groove 231. The movement of the limiter 21 is arranged in the placement groove 231. An elastic member 22 is provided between the limiter 21 and the placement groove 231. The limiter 21 is driven by the elastic member 22 to press the ELISA plate 3 against the inner wall of the fixed cabin 2.

[0038] In other embodiments of the present application, unlike the above-mentioned method of using the elastic member 22 to maintain the position of the limit member 21, the movement of part of the limit member 21 is set in the placement groove 231, and the outer wall of the limit member 21 is provided with a thread. When the limit member 21 rotates around its own axis, the length of the part in the placement groove 231 changes.

[0039] In some embodiments of the present application, Figure 1-10 As shown, the limiting member 21 includes a push plate 211, the height of the push plate 211 in the placement groove 231 is greater than the thickness of the ELISA plate 3, and the end of the push plate 211 facing the ELISA plate 3 has an inclined surface facing the bottom of the placement groove 231. When the limiting member 21 is pressed against the ELISA plate 3, the push plate 211 provides a component force to press the ELISA plate 3 toward the bottom of the placement groove 231.

[0040] In some embodiments of the present application, Figure 1-10As shown, the operating end 12 has a boss 121 surrounding the outside of the conical air duct 11, and the area of the outer contour of the boss 121 is not less than the area of the contact end 232; the connecting basket 24 includes adapter plates 241 symmetrically arranged on both sides of the cabin body 23. When the fixed cabin 2 moves to the working position, the contact end 232 contacts the boss 121, and the adapter plates 241 block the gap 233 on both sides of the cabin body 23 and match the edges of the operating end 12 or the boss 121 to prevent the droplets in the enzyme labeling hole 31 from splashing outward through the gap 233 when blowing. The contact end 232 of the cabin body 23 has an upper end face, which coincides with the upper end face of the boss 121 when the fixed cabin 2 is in the working position, thereby preventing liquid from splashing out from other outer walls of the cabin body 23 that are not in contact with the adapter plates 241.

[0041] In some embodiments of the present application, Figure 1-10 As shown, the connecting basket 24 also includes a baffle 242, which is connected to the adapter 241. When the cabin 23 contacts the boss 121, the baffle 242 presses against the end of the cabin 23 away from the contact end 232, thereby pressing the cabin 23 against the operating end 12 through the weight of the connecting basket 24 to prevent the enzyme sample from being lifted up when the airflow blows through the enzyme label hole 31.

[0042] In some embodiments of the present application, Figure 1-10 As shown, the operating end portion 12 is provided with a water diversion groove 122, which is evenly distributed on the operating end portion 12 and divides the tapered air duct 11 into several groups. When the tapered air duct 11 sprays air into the enzyme-labeled hole 31, an operating space is formed between the cabin 23 and the operating end portion 12 to prevent liquid droplets from splashing. Therefore, the airflow in the operating space can, to a certain extent, push the liquid gathered in the water diversion groove 122 into the water collection groove 123 and the drainage channel 13. The operating end portion 12 is also provided with a water collection groove 123. The water diversion grooves 122 are all connected to the water collection groove 123. After the liquid droplets enter the water diversion groove 122, they move toward the water collection groove 123.

[0043] In some embodiments of the present application, Figure 1-10 As shown, the clean plate bellows 1 is provided with a drainage channel 13 extending therethrough, which is connected to a water collection tank 123. When the fixed cabin 2 is in the working position, the airflow ejected from the tapered air duct 11 drives the droplets in the enzyme-labeled wells 31 to slide along the outer wall of the tapered air duct 11 toward the operating end 12 and into the water diversion tank 122. A drainage pipe 14 is connected to the outer wall of the clean plate bellows 1, which is connected to the drainage channel 13.

[0044] In some embodiments of the present application, Figure 1-10As shown, the device also includes a shock-absorbing pad 5 and a base 6. The shock-absorbing pad 5 is connected between the clean plate bellows 1 and the base 6. When the oscillator 4 is operating, the shock-absorbing pad 5 reduces the oscillating motion of the base 6 to prevent the entire variable frequency clean plate device from moving relative to the operating environment during oscillation, which could cause the device to fall and be damaged. The shock-absorbing pad 5 is connected to a power switch 51 and a power interface 52. Lead wires connect the power switch 51 to the power interface 52, the power switch 51 to the oscillator 4, and the power switch 51 to the fan 152. When the power interface 52 is connected to an external power source, turning on the power switch 51 activates the oscillator 4 and the fan 152. The shock-absorbing pad 5 is made of a rubber material, providing both shock absorption and fatigue resistance. The base 6 is made of metal to provide sufficient stability.

[0045] In some embodiments of the present application, Figure 1-10 As shown, an air flow channel 15 is provided in the clean plate air box 1, and the air flow channel 15 is connected to an air inlet 151 provided on the outer wall of the clean plate air box 1, and a fan 152 is provided in the air flow channel 15; when the fan 152 is working, the external air flow enters the clean plate air box 1 from the air inlet 151 and is compressed at the conical air duct 11 and then ejected out at an accelerated speed.

[0046] In some embodiments of the present application, Figure 1-10 As shown, the power switch 51 is connected to the power line interface 42 through a wire, and is also connected to the plate cleaning bellows 1 and the oscillator 4 through a wire, and is controlled to start and shut down the ELISA enzyme plate 3 frequency conversion plate cleaning device by opening and closing.

Claims

1. An ELISA plate frequency conversion cleaning device, characterized in that: include, A clean plate bellows provides a moving air flow, and a plurality of tapered air duct arrays are provided at the operating end of the clean plate bellows, and the clean plate bellows are connected to the tapered air ducts; A fixed cabin is rotatably connected to the clean plate bellows, and a limit member is provided for movement in the fixed cabin. When the limit member moves to a limit position, the relative position relationship between the fixed cabin and the ELISA plate in the fixed cabin is locked. When the fixed cabin rotates to a working position, the tapered air ducts extend into the ELISA holes one by one. An oscillator is connected to the operating end. The vibration frequency of the oscillator is less than 10,000 Hz and the amplitude is not less than 0.5 mm. When the oscillator vibrates, it overcomes the wetting force between the droplet and the enzyme-labeled hole. When the air flow is accelerated from the clean plate bellows through the conical air duct into the enzyme-labeled hole, it drives the droplet to break away from the enzyme-labeled hole and move toward the operating end.

2. An ELISA plate frequency conversion cleaning device according to claim 1, characterized in that, The fixed cabin has a placement groove, the movement of the limiting part is arranged in the placement groove, an elastic part is arranged between the limiting part and the placement groove, and the limiting part presses the ELISA plate and the inner wall of the fixed cabin tightly under the drive of the elastic part.

3. An ELISA plate frequency conversion cleaning device according to claim 2, characterized in that, The limiting member includes a push plate, the height of which in the placement groove is greater than the thickness of the ELISA plate, and the end of the push plate facing the ELISA plate has an inclined surface facing the bottom of the placement groove. When the limiting member presses against the ELISA plate, the push plate provides a component force to press the ELISA plate toward the bottom of the placement groove.

4. The ELISA plate frequency conversion and cleaning device according to claim 1, characterized in that: The fixed cabin includes a cabin body and a connection basket that are rotatably connected. The connection basket is rotatably connected to the operating end. The cabin body has a contact end. When the fixed cabin moves to the working position, the contact end contacts the operating end.

5. The ELISA plate frequency conversion and cleaning device according to claim 4, characterized in that: The operating end has a boss surrounding the outside of the conical air duct, and the area of the outer contour of the boss is not less than the area of the contact end; the connecting basket includes adapter plates symmetrically arranged on both sides of the cabin body. When the fixed cabin moves to the working position, the contact end contacts the boss, and the adapter plates block the gap on both sides of the cabin body.

6. An ELISA plate frequency conversion cleaning device according to claim 1, characterized in that: The operating end is provided with a water diversion groove, which is evenly distributed at the operating end and divides the conical air duct into several groups; the operating end is also provided with a water collection groove, and the water diversion grooves are all connected to the water collection groove. After the droplets enter the water diversion groove, they move towards the water collection groove.

7. An ELISA plate frequency conversion cleaning device according to claim 6, characterized in that: The clean plate bellows is penetrated by a drainage channel which is connected to the water collecting trough. When the fixed cabin is in the working position, the airflow ejected from the conical air duct drives the droplets in the enzyme labeling holes to slide along the outer wall of the conical air duct toward the operating end and into the water diversion trough.

8. The ELISA plate frequency conversion cleaning device according to claim 1, characterized in that: The movement of the limiting member portion is arranged in the placement groove, the outer wall of the limiting member is provided with a thread, and when the limiting member rotates around its own axis, the length of the portion located in the placement groove changes.

9. The ELISA plate frequency conversion cleaning device according to claim 1, characterized in that: It also includes a shock-absorbing pad and a bottom bracket. The shock-absorbing pad is connected between the clean plate bellows and the bottom bracket. When the oscillator is working, the shock-absorbing pad reduces the oscillating movement of the bottom bracket.

10. The ELISA plate frequency conversion cleaning device according to claim 1, characterized in that: An air flow channel is provided in the clean plate bellows, and the air flow channel is connected to an air inlet provided on the outer wall of the clean plate bellows. A fan is provided in the air flow channel; when the fan is working, the external air flow enters the clean plate bellows from the air inlet and is compressed at the conical air duct and then ejected out at an accelerated speed.

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