A composite plant extract coated microcapsule, a preparation method thereof and application thereof in preparing an anti-hair loss essential oil

Abstract

The present application relates to a kind of composite plant extract coated microcapsules and its preparation method and application in the preparation of anti-hair loss hair growth oil, belong to hair preparation technical field.The present application uses 2,6-dimethyl-β-cyclodextrin, γ-cyclodextrin, plant extract and ginger essential oil to prepare coated microcapsules;2,6-dimethyl-β-cyclodextrin, γ-cyclodextrin can be matched to give microcapsules significantly improved coating effect, increase the efficacy and sustained-release of microcapsules, improve the efficacy of microcapsules against hair loss;Plant extract and ginger essential oil as active ingredient, so that microcapsule can effectively promote hair growth and prevent hair loss.

Description

Technical Field

[0001] This invention relates to a composite plant extract encapsulated microcapsule, its preparation method, and its application in the preparation of anti-hair loss and hair growth essential oil, belonging to the field of hair preparation technology. Background Technology

[0002] Plant extract encapsulation microcapsules are a technology that encapsulates the active ingredients of plant extracts within tiny carriers using microencapsulation technology. Microcapsules typically consist of a wall material (encapsulating material) and a core material (plant extract). The wall material is often a natural or synthetic polymer (such as gelatin, chitosan, liposomes, polylactic acid, etc.), forming tiny particles that protect the core material from external environmental influences.

[0003] Microencapsulation can protect active ingredients from degradation caused by oxidation, light, high temperatures, or acidic / alkaline environments; control release by using wall material design for targeted or sustained release, extending the duration of action; improve stability by addressing the volatility and storage difficulties of liquid extracts; enhance applicability by masking bitterness or irritating odors, facilitating processing into solid dosage forms (such as tablets or powders); and enhance bioavailability by promoting absorption of ingredients in the body through nanoscale microcapsules. Microencapsulation can be applied in specific fields such as cosmetics, food, pharmaceuticals, and agriculture.

[0004] Hair loss prevention agents are functional products that reduce hair loss and promote hair regeneration by regulating the scalp environment, activating hair follicle activity, or inhibiting hair loss-related mechanisms (such as hormones and inflammation). Plant extracts, due to their natural nature and low side effects, are important components of these agents. Their functions include: inhibiting hair loss-related pathways, inhibiting DHT production, improving androgenetic alopecia, anti-oxidation, inhibiting 5α-reductase activity, and reducing hair follicle atrophy; promoting hair growth, activating dermal papilla cells, promoting hair follicle proliferation and angiogenesis, and prolonging the hair growth phase; improving the scalp environment, antibacterial and anti-inflammatory effects, alleviating seborrheic alopecia, promoting local blood circulation, and enhancing nutrient supply to hair follicles.

[0005] Compared to synthetic drugs (such as minoxidil, which may cause scalp irritation), plant-based ingredients are gentler and suitable for long-term use; plant extracts often contain a variety of active ingredients (such as flavonoids, saponins, and polysaccharides), which can simultaneously reduce inflammation, promote circulation, and provide antioxidant benefits; the natural ingredients of plants align with the "clean beauty" trend, increasing the market acceptance of the products.

[0006] Microencapsulating plant extracts in hair loss treatments is a current research hotspot. This approach can improve stability, inhibit the degradation of active ingredients, prolong activity through microencapsulation with liposomes, enhance permeability, promote the penetration of active ingredients across the scalp barrier, control release, target hair follicle areas, reduce ingredient waste, and achieve highly effective results. However, developing a method to encapsulate compound plant extracts in microcapsules and apply it to anti-hair loss products to enhance their effectiveness remains a technical challenge that needs to be addressed. Summary of the Invention

[0007] To address the aforementioned technical problems, this invention provides a composite plant extract encapsulated microcapsule, its preparation method, and its application in the preparation of anti-hair loss and hair growth essential oil.

[0008] This invention provides a composite plant extract-encapsulated microcapsule, comprising 2,6-dimethyl-β-cyclodextrin, γ-cyclodextrin, plant extracts, and ginger essential oil. The combination of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin significantly enhances the encapsulation effect of the microcapsules, increasing their efficacy and sustained-release properties, and improving their anti-hair loss and hair growth effects. The plant extracts and ginger essential oil, as active ingredients, enable the microcapsules to effectively promote hair growth and prevent hair loss.

[0009] In a preferred embodiment, the compound plant extract is encapsulated in microcapsules, and the raw materials, by weight, include 5-10 parts of 2,6-dimethyl-β-cyclodextrin, 4-8 parts of γ-cyclodextrin, 0.8-2.5 parts of plant extract and 0.2-0.5 parts of ginger essential oil.

[0010] In a preferred embodiment, the microcapsules containing the compound plant extract, with a weight ratio of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin of 1:0.6-0.8, can obtain microcapsules with better preservation and sustained-release effects, thus giving the microcapsules better efficacy.

[0011] In a preferred embodiment, the method for preparing the plant extract includes: (1) pulverizing arborvitae leaves and Polygonum multiflorum, heating and mixing with water, and then cooling; (2) adding glucose and Listeria monocytogenes ATCC33090 to obtain fermentation material, fermenting to obtain fermentation product, sterilizing, and concentrating; (3) adding ethanol to obtain a mixture, heating and extracting, filtering, concentrating the filtrate, and drying to obtain the plant extract.

[0012] In a preferred embodiment, the weight ratio of Platycladus orientalis leaves to Polygonum multiflorum is 1:0.8-1.5.

[0013] In a preferred embodiment, the total weight ratio of arborvitae leaves and Polygonum multiflorum to water is 1:10-30.

[0014] In a preferred embodiment, cooling after heating and mixing includes cooling after heating and boiling.

[0015] In a preferred embodiment, the temperature is cooled to 15-30°C.

[0016] In a preferred embodiment, glucose is used in the fermentation feed at an amount of 1-4.4 wt%.

[0017] In a preferred embodiment, the amount of Listeria innocense ATCC33090 in the fermentation feed is 10. 10 Up to 10 13 per g.

[0018] In a preferred embodiment, the fermentation temperature and time are 25-40°C for 3-7 days.

[0019] In a preferred embodiment, the concentration includes vacuum concentration.

[0020] In a preferred embodiment, (2) is concentrated to a weight of 1.5-5 times the total weight of the arborvitae leaves and Polygonum multiflorum.

[0021] In a preferred embodiment, the concentration of ethanol in the mixture is 65-80 wt%.

[0022] In a preferred embodiment, the temperature and time for heating and extraction are 40-60°C and 3-10 hours.

[0023] In a preferred embodiment, (3) is concentrated to a weight of 0.1-0.25 times that of the filtrate.

[0024] The present invention also provides a method for preparing the composite plant extract encapsulated microcapsules, comprising the following steps: heating and stirring 2,6-dimethyl-β-cyclodextrin, γ-cyclodextrin and water; adding plant extracts and ginger essential oil, stirring, then homogenizing under high pressure, concentrating, and drying to obtain composite plant extract encapsulated microcapsules.

[0025] In a preferred embodiment, the weight ratio of water to the total weight of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin is 1:40-150.

[0026] In a preferred embodiment, the heating and stirring temperature and time are 30-45°C for 0.5-2 hours.

[0027] The present invention also provides the application of the aforementioned composite plant extract encapsulated microcapsules in the preparation of cosmetics or hair loss prevention and hair growth drugs.

[0028] The present invention also provides the application of the aforementioned composite plant extract encapsulated in microcapsules in the preparation of anti-hair loss and hair growth essential oil.

[0029] Compared to existing technologies, the advancement of this invention lies in:

[0030] This invention uses 2,6-dimethyl-β-cyclodextrin, γ-cyclodextrin, plant extracts, and ginger essential oil to prepare encapsulated microcapsules. The combination of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin can give the microcapsules a significantly enhanced encapsulation effect, increase the efficacy and sustained-release properties of the microcapsules, and improve their anti-hair loss and hair growth efficacy. Plant extracts and ginger essential oil serve as active ingredients, enabling the microcapsules to effectively promote hair growth and prevent hair loss.

[0031] The microcapsules prepared by this invention can effectively inhibit the activity of type II 5α reductase, and have the effect of preventing hair loss and promoting hair growth.

[0032] This invention utilizes Listeria monocytogenes ATCC33090 to ferment plant extracts, which effectively enhances the activity of microcapsules inhibiting type II 5α-reductase. The use of this fermenting bacterium enriches the types and concentrations of active ingredients inhibiting type II 5α-reductase activity, giving the microcapsules superior effects. Compared to other bacteria, the Listeria monocytogenes ATCC33090 used in this invention exhibits better hair growth promotion and anti-hair loss effects. The selection of specific fermenting bacteria in this invention effectively enhances the efficacy of the microcapsules.

[0033] The present invention uses high-concentration ethanol extraction, which can effectively improve the microencapsulation effect, and the effect is even better when the concentration of ethanol extraction is controlled at 65-80wt%.

[0034] This invention utilizes 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin to synergistically enhance the stability of microcapsules, thereby maintaining the efficacy of the microcapsules against type II 5α-reductase activity inhibition. The efficacy retention is even better when the weight ratio of 2,6-dimethyl-β-cyclodextrin to γ-cyclodextrin is 1:0.6-0.8. Detailed Implementation

[0035] This invention demonstrates its technology through specific embodiments, which provide a clearer and more complete understanding of the technical content of this invention.

[0036] This invention provides a composite plant extract-encapsulated microcapsule, comprising 2,6-dimethyl-β-cyclodextrin, γ-cyclodextrin, plant extracts, and ginger essential oil. The combination of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin significantly enhances the encapsulation effect of the microcapsules, increasing their efficacy and uniform release, and improving their anti-hair loss and hair growth effects. The plant extracts and ginger essential oil, as active ingredients, enable the microcapsules to effectively promote hair growth and prevent hair loss.

[0037] In a preferred embodiment, the compound plant extract is encapsulated in microcapsules, and the raw materials, by weight, include 5-10 parts of 2,6-dimethyl-β-cyclodextrin, 4-8 parts of γ-cyclodextrin, 0.8-2.5 parts of plant extract and 0.2-0.5 parts of ginger essential oil.

[0038] In a preferred embodiment, the microcapsules containing the compound plant extract, with a weight ratio of 2,6-dimethyl-β-cyclodextrin to γ-cyclodextrin of 1:0.6-0.8, can produce microcapsules with better preservation and uniform release, thus giving the microcapsules better efficacy.

[0039] In a preferred embodiment, the method for preparing the plant extract includes: (1) pulverizing arborvitae leaves and Polygonum multiflorum, heating and mixing with water, and then cooling; (2) adding glucose and Listeria monocytogenes ATCC33090 to obtain fermentation material, fermenting to obtain fermentation product, sterilizing, and concentrating; (3) adding ethanol to obtain a mixture, heating and extracting, filtering, concentrating the filtrate, and drying to obtain the plant extract.

[0040] In a preferred embodiment, the weight ratio of Platycladus orientalis leaves to Polygonum multiflorum is 1:0.8-1.5.

[0041] In a preferred embodiment, the total weight ratio of arborvitae leaves and Polygonum multiflorum to water is 1:10-30.

[0042] In a preferred embodiment, cooling after heating and mixing includes cooling after heating and boiling.

[0043] In a preferred embodiment, the temperature is cooled to 15-30°C.

[0044] In a preferred embodiment, glucose is used in the fermentation feed at an amount of 1-4.4 wt%.

[0045] In a preferred embodiment, the amount of Listeria innocense ATCC33090 in the fermentation feed is 10. 10 Up to 10 13 per g.

[0046] In a preferred embodiment, the fermentation temperature and time are 25-40°C for 3-7 days.

[0047] In a preferred embodiment, the concentration includes vacuum concentration.

[0048] In a preferred embodiment, (2) is concentrated to a weight of 1.5-5 times the total weight of the arborvitae leaves and Polygonum multiflorum.

[0049] In a preferred embodiment, the concentration of ethanol in the mixture is 65-80 wt%.

[0050] In a preferred embodiment, the temperature and time for heating and extraction are 40-60°C and 3-10 hours.

[0051] In a preferred embodiment, (3) is concentrated to a weight of 0.1-0.25 times that of the filtrate.

[0052] The present invention also provides a method for preparing the composite plant extract encapsulated microcapsules, comprising the following steps: heating and stirring 2,6-dimethyl-β-cyclodextrin, γ-cyclodextrin and water; adding plant extracts and ginger essential oil, stirring, then homogenizing under high pressure, concentrating, and drying to obtain composite plant extract encapsulated microcapsules.

[0053] In a preferred embodiment, the weight ratio of water to the total weight of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin is 1:40-150.

[0054] In a preferred embodiment, the heating and stirring temperature and time are 30-45°C for 0.5-2 hours.

[0055] The present invention also provides the application of the aforementioned composite plant extract encapsulated microcapsules in the preparation of cosmetics or hair loss prevention and hair growth drugs.

[0056] The present invention also provides the application of the aforementioned composite plant extract encapsulated in microcapsules in the preparation of anti-hair loss and hair growth essential oil.

[0057] Specific examples of the present invention are as follows.

[0058] 2,6-Dimethyl-β-cyclodextrin: Zibo Qianhui Biotechnology Co., Ltd. γ-Cyclodextrin: Leshengyuan Biotechnology (Nanjing) Co., Ltd. Listeria Innolux ATCC33090: Xinyang Laiyao Biotechnology Co., Ltd. Ginger essential oil: Wuhan Jiangxin Biotechnology Co., Ltd.

[0059] Preparation Example 1:

[0060] The preparation method is as follows: Weigh the raw materials according to the following weight parts: 8.0 parts of 2,6-dimethyl-β-cyclodextrin, 6.4 parts of γ-cyclodextrin, 2.0 parts of plant extract and 0.4 parts of ginger essential oil; the weight ratio of 2,6-dimethyl-β-cyclodextrin to γ-cyclodextrin is 1:0.8;

[0061] The preparation method of the plant extract is as follows:

[0062] (1) Pulverize the arborvitae leaves and Polygonum multiflorum in a weight ratio of 1:1.2, then heat and boil them with water in a weight ratio of 1:20 for the total weight of the arborvitae leaves and Polygonum multiflorum, and then cool them to 25°C.

[0063] (2) Add glucose and Listeria innocense ATCC33090 to obtain the fermentation broth (the amounts of glucose and Listeria innocense ATCC33090 in the fermentation broth were 3.28 wt% and 5.5 × 10⁻⁶ wt%, respectively). 12The product was obtained by aerobic fermentation at 30℃ for 5 days (air flow rate 0.3L / (min·1L fermentation space)), sterilized, and concentrated under reduced pressure at 55℃ to a weight of twice the total weight of the arborvitae leaves and Polygonum multiflorum.

[0064] (3) Add ethanol to obtain a mixture (the concentration of ethanol in the mixture is 70wt%), heat at 50℃ and stir at 60rpm for 5 hours, filter with a 0.22μm filter membrane, concentrate the filtrate under reduced pressure at 50℃ to 0.15 times the weight of the filtrate, freeze dry at -40℃ to a water content of 1.27wt%, and obtain the plant extract.

[0065] 2,6-Dimethyl-β-cyclodextrin, γ-cyclodextrin, and water (the weight ratio of water to the total weight of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin was 1:55) were heated at 35°C and stirred at 60 rpm for 0.6 hours. Plant extracts and ginger essential oil were added, and the mixture was stirred at 500 rpm for 0.6 hours. Then, the mixture was homogenized under high pressure (100 MPa, 3 cycles, 30 L / min, homogenization temperature 35°C), concentrated under reduced pressure at 50°C to 16.7% of the homogenate weight, and freeze-dried at -40°C to 2.13 wt% water content to obtain microcapsules containing the compound plant extracts.

[0066] Preparation Example 2:

[0067] The preparation method is as follows: Weigh the raw materials according to the following weight parts: 7.9 parts of 2,6-dimethyl-β-cyclodextrin, 4.8 parts of γ-cyclodextrin, 1.75 parts of plant extract and 0.45 parts of ginger essential oil; the weight ratio of 2,6-dimethyl-β-cyclodextrin to γ-cyclodextrin is 1:0.61;

[0068] The preparation method of the plant extract is as follows:

[0069] (1) Pulverize the arborvitae leaves and Polygonum multiflorum in a weight ratio of 1:0.8, then heat and boil them with water in a weight ratio of 1:22, and then cool them to 25°C.

[0070] (2) Add glucose and Listeria innocense ATCC33090 to obtain the fermentation broth (the amounts of glucose and Listeria innocense ATCC33090 in the fermentation broth are 3.05 wt% and 6 × 10⁻⁶ wt%, respectively). 12 The fermentation product was obtained by aerobic fermentation at 32℃ for 4.5 days (air flow rate 0.3L / (min·1L fermentation space)), sterilized, and concentrated under reduced pressure at 55℃ to a weight of 2.1 times the total weight of the arborvitae leaves and Polygonum multiflorum.

[0071] (3) Add ethanol to obtain a mixture (the concentration of ethanol in the mixture is 80wt%), heat at 50℃ and stir at 60rpm for 5.5 hours, filter with a 0.22μm filter membrane, concentrate the filtrate under reduced pressure at 50℃ to 0.14 times the weight of the filtrate, freeze dry at -40℃ to a water content of 1.21wt%, and obtain the plant extract.

[0072] 2,6-Dimethyl-β-cyclodextrin, γ-cyclodextrin, and water (the weight ratio of water to the total weight of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin was 1:50) were heated at 35°C and stirred at 60 rpm for 0.7 hours. Plant extracts and ginger essential oil were added, and the mixture was stirred at 400 rpm for 0.7 hours. Then, the mixture was homogenized under high pressure (100 MPa, 3 cycles, 30 L / min, homogenization temperature 35°C), concentrated under reduced pressure at 50°C to 16.2% by weight of the homogenate, and freeze-dried at -40°C to 2.18 wt% water content to obtain microcapsules containing the compound plant extracts.

[0073] Preparation Example 3:

[0074] The preparation method is as follows: Weigh the raw materials according to the following weight parts: 14.4 parts of 2,6-dimethyl-β-cyclodextrin, 2.0 parts of plant extract and 0.4 parts of ginger essential oil; the weight ratio of 2,6-dimethyl-β-cyclodextrin to γ-cyclodextrin is 1:0.8;

[0075] The preparation method of the plant extract is as follows:

[0076] (1) Pulverize the arborvitae leaves and Polygonum multiflorum in a weight ratio of 1:1.2, then heat and boil them with water in a weight ratio of 1:20 for the total weight of the arborvitae leaves and Polygonum multiflorum, and then cool them to 25°C.

[0077] (2) Add glucose and Listeria innocense ATCC33090 to obtain the fermentation broth (the amounts of glucose and Listeria innocense ATCC33090 in the fermentation broth were 3.28 wt% and 5.5 × 10⁻⁶ wt%, respectively). 12 The product was obtained by aerobic fermentation at 30℃ for 5 days (air flow rate 0.3L / (min·1L fermentation space)), sterilized, and concentrated under reduced pressure at 55℃ to a weight of twice the total weight of the arborvitae leaves and Polygonum multiflorum.

[0078] (3) Add ethanol to obtain a mixture (the concentration of ethanol in the mixture is 70wt%), heat at 50℃ and stir at 60rpm for 5 hours, filter with a 0.22μm filter membrane, concentrate the filtrate under reduced pressure at 50℃ to 0.15 times the weight of the filtrate, freeze dry at -40℃ to a water content of 1.27wt%, and obtain the plant extract.

[0079] 2,6-Dimethyl-β-cyclodextrin, γ-cyclodextrin, and water (the weight ratio of water to the total weight of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin was 1:55) were heated at 35°C and stirred at 60 rpm for 0.6 hours. Plant extracts and ginger essential oil were added, and the mixture was stirred at 500 rpm for 0.6 hours. Then, the mixture was homogenized under high pressure (100 MPa, 3 cycles, 30 L / min, homogenization temperature 35°C), concentrated under reduced pressure at 50°C to 16.7% of the homogenate weight, and freeze-dried at -40°C to 2.13 wt% water content to obtain microcapsules containing the compound plant extracts.

[0080] Preparation Example 4:

[0081] The preparation method is as follows: Weigh the raw materials according to the following weight parts: 14.4 parts γ-cyclodextrin, 2.0 parts plant extract and 0.4 parts ginger essential oil; the weight ratio of 2,6-dimethyl-β-cyclodextrin to γ-cyclodextrin is 1:0.8;

[0082] The preparation method of the plant extract is as follows:

[0083] (1) Pulverize the arborvitae leaves and Polygonum multiflorum in a weight ratio of 1:1.2, then heat and boil them with water in a weight ratio of 1:20 for the total weight of the arborvitae leaves and Polygonum multiflorum, and then cool them to 25°C.

[0084] (2) Add glucose and Listeria innocense ATCC33090 to obtain the fermentation broth (the amounts of glucose and Listeria innocense ATCC33090 in the fermentation broth were 3.28 wt% and 5.5 × 10⁻⁶ wt%, respectively). 12 The product was obtained by aerobic fermentation at 30℃ for 5 days (air flow rate 0.3L / (min·1L fermentation space)), sterilized, and concentrated under reduced pressure at 55℃ to a weight of twice the total weight of the arborvitae leaves and Polygonum multiflorum.

[0085] (3) Add ethanol to obtain a mixture (the concentration of ethanol in the mixture is 70wt%), heat at 50℃ and stir at 60rpm for 5 hours, filter with a 0.22μm filter membrane, concentrate the filtrate under reduced pressure at 50℃ to 0.15 times the weight of the filtrate, freeze dry at -40℃ to a water content of 1.27wt%, and obtain the plant extract.

[0086] 2,6-Dimethyl-β-cyclodextrin, γ-cyclodextrin, and water (the weight ratio of water to the total weight of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin was 1:55) were heated at 35°C and stirred at 60 rpm for 0.6 hours. Plant extracts and ginger essential oil were added, and the mixture was stirred at 500 rpm for 0.6 hours. Then, the mixture was homogenized under high pressure (100 MPa, 3 cycles, 30 L / min, homogenization temperature 35°C), concentrated under reduced pressure at 50°C to 16.7% of the homogenate weight, and freeze-dried at -40°C to 2.13 wt% water content to obtain microcapsules containing the compound plant extracts.

[0087] Preparation Example 5:

[0088] The preparation method is as follows: Weigh the raw materials according to the following weight parts: 12 parts of 2,6-dimethyl-β-cyclodextrin, 2.4 parts of γ-cyclodextrin, 2.0 parts of plant extract and 0.4 parts of ginger essential oil; the weight ratio of 2,6-dimethyl-β-cyclodextrin to γ-cyclodextrin is 1:0.2;

[0089] The preparation method of the plant extract is as follows:

[0090] (1) Pulverize the arborvitae leaves and Polygonum multiflorum in a weight ratio of 1:1.2, then heat and boil them with water in a weight ratio of 1:20 for the total weight of the arborvitae leaves and Polygonum multiflorum, and then cool them to 25°C.

[0091] (2) Add glucose and Listeria innocense ATCC33090 to obtain the fermentation broth (the amounts of glucose and Listeria innocense ATCC33090 in the fermentation broth were 3.28 wt% and 5.5 × 10⁻⁶ wt%, respectively). 12 The product was obtained by aerobic fermentation at 30℃ for 5 days (air flow rate 0.3L / (min·1L fermentation space)), sterilized, and concentrated under reduced pressure at 55℃ to a weight of twice the total weight of the arborvitae leaves and Polygonum multiflorum.

[0092] (3) Add ethanol to obtain a mixture (the concentration of ethanol in the mixture is 70wt%), heat at 50℃ and stir at 60rpm for 5 hours, filter with a 0.22μm filter membrane, concentrate the filtrate under reduced pressure at 50℃ to 0.15 times the weight of the filtrate, freeze dry at -40℃ to a water content of 1.27wt%, and obtain the plant extract.

[0093] 2,6-Dimethyl-β-cyclodextrin, γ-cyclodextrin, and water (the weight ratio of water to the total weight of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin was 1:55) were heated at 35°C and stirred at 60 rpm for 0.6 hours. Plant extracts and ginger essential oil were added, and the mixture was stirred at 500 rpm for 0.6 hours. Then, the mixture was homogenized under high pressure (100 MPa, 3 cycles, 30 L / min, homogenization temperature 35°C), concentrated under reduced pressure at 50°C to 16.7% of the homogenate weight, and freeze-dried at -40°C to 2.13 wt% water content to obtain microcapsules containing the compound plant extracts.

[0094] Preparation Example 6:

[0095] The preparation method is as follows: Weigh the raw materials according to the following weight parts: 3.6 parts of 2,6-dimethyl-β-cyclodextrin, 10.8 parts of γ-cyclodextrin, 2.0 parts of plant extract and 0.4 parts of ginger essential oil; the weight ratio of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin is 1:3;

[0096] The preparation method of the plant extract is as follows:

[0097] (1) Pulverize the arborvitae leaves and Polygonum multiflorum in a weight ratio of 1:1.2, then heat and boil them with water in a weight ratio of 1:20 for the total weight of the arborvitae leaves and Polygonum multiflorum, and then cool them to 25°C.

[0098] (2) Add glucose and Listeria innocense ATCC33090 to obtain the fermentation broth (the amounts of glucose and Listeria innocense ATCC33090 in the fermentation broth were 3.28 wt% and 5.5 × 10⁻⁶ wt%, respectively). 12 The product was obtained by aerobic fermentation at 30℃ for 5 days (air flow rate 0.3L / (min·1L fermentation space)), sterilized, and concentrated under reduced pressure at 55℃ to a weight of twice the total weight of the arborvitae leaves and Polygonum multiflorum.

[0099] (3) Add ethanol to obtain a mixture (the concentration of ethanol in the mixture is 70wt%), heat at 50℃ and stir at 60rpm for 5 hours, filter with a 0.22μm filter membrane, concentrate the filtrate under reduced pressure at 50℃ to 0.15 times the weight of the filtrate, freeze dry at -40℃ to a water content of 1.27wt%, and obtain the plant extract.

[0100] 2,6-Dimethyl-β-cyclodextrin, γ-cyclodextrin, and water (the weight ratio of water to the total weight of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin was 1:55) were heated at 35°C and stirred at 60 rpm for 0.6 hours. Plant extracts and ginger essential oil were added, and the mixture was stirred at 500 rpm for 0.6 hours. Then, the mixture was homogenized under high pressure (100 MPa, 3 cycles, 30 L / min, homogenization temperature 35°C), concentrated under reduced pressure at 50°C to 16.7% of the homogenate weight, and freeze-dried at -40°C to 2.13 wt% water content to obtain microcapsules containing the compound plant extracts.

[0101] Preparation Example 7:

[0102] The preparation method is as follows: Weigh the raw materials according to the following weight parts: 8.0 parts of 2,6-dimethyl-β-cyclodextrin, 6.4 parts of γ-cyclodextrin, 2.0 parts of plant extract and 0.4 parts of ginger essential oil; the weight ratio of 2,6-dimethyl-β-cyclodextrin to γ-cyclodextrin is 1:0.8;

[0103] The preparation method of the plant extract is as follows:

[0104] (1) Pulverize the arborvitae leaves and Polygonum multiflorum in a weight ratio of 1:1.2, then heat and boil them with water in a weight ratio of 1:20 for the total weight of the arborvitae leaves and Polygonum multiflorum, and then cool them to 25°C.

[0105] (2) The cooled material was concentrated at 55℃ under reduced pressure until its weight was twice the total weight of the arborvitae leaves and Polygonum multiflorum.

[0106] (3) Add ethanol to obtain a mixture (the concentration of ethanol in the mixture is 70wt%), heat at 50℃ and stir at 60rpm for 5 hours, filter with a 0.22μm filter membrane, concentrate the filtrate under reduced pressure at 50℃ to 0.15 times the weight of the filtrate, freeze dry at -40℃ to a water content of 1.27wt%, and obtain the plant extract.

[0107] 2,6-Dimethyl-β-cyclodextrin, γ-cyclodextrin, and water (the weight ratio of water to the total weight of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin was 1:55) were heated at 35°C and stirred at 60 rpm for 0.6 hours. Plant extracts and ginger essential oil were added, and the mixture was stirred at 500 rpm for 0.6 hours. Then, the mixture was homogenized under high pressure (100 MPa, 3 cycles, 30 L / min, homogenization temperature 35°C), concentrated under reduced pressure at 50°C to 16.7% of the homogenate weight, and freeze-dried at -40°C to 2.13 wt% water content to obtain microcapsules containing the compound plant extracts.

[0108] Preparation Example 8:

[0109] The preparation method is as follows: Weigh the raw materials according to the following weight parts: 8.0 parts of 2,6-dimethyl-β-cyclodextrin, 6.4 parts of γ-cyclodextrin, 2.0 parts of plant extract and 0.4 parts of ginger essential oil; the weight ratio of 2,6-dimethyl-β-cyclodextrin to γ-cyclodextrin is 1:0.8;

[0110] The preparation method of the plant extract is as follows:

[0111] (1) Pulverize the arborvitae leaves and Polygonum multiflorum in a weight ratio of 1:1.2, then heat and boil them with water in a weight ratio of 1:20 for the total weight of the arborvitae leaves and Polygonum multiflorum, and then cool them to 25°C.

[0112] (2) Add glucose and Bacillus subtilis CICC 10262 to obtain the fermentation substrate (the amounts of glucose and Bacillus subtilis in the fermentation substrate are 3.28 wt% and 5.5 × 10⁻⁶, respectively). 12 The product was obtained by aerobic fermentation at 30℃ for 5 days (air flow rate 0.3L / (min·1L fermentation space)), sterilized, and concentrated under reduced pressure at 55℃ to a weight of twice the total weight of the arborvitae leaves and Polygonum multiflorum.

[0113] (3) Add ethanol to obtain a mixture (the concentration of ethanol in the mixture is 70wt%), heat at 50℃ and stir at 60rpm for 5 hours, filter with a 0.22μm filter membrane, concentrate the filtrate under reduced pressure at 50℃ to 0.15 times the weight of the filtrate, freeze dry at -40℃ to a water content of 1.27wt%, and obtain the plant extract.

[0114] 2,6-Dimethyl-β-cyclodextrin, γ-cyclodextrin, and water (the weight ratio of water to the total weight of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin was 1:55) were heated at 35°C and stirred at 60 rpm for 0.6 hours. Plant extracts and ginger essential oil were added, and the mixture was stirred at 500 rpm for 0.6 hours. Then, the mixture was homogenized under high pressure (100 MPa, 3 cycles, 30 L / min, homogenization temperature 35°C), concentrated under reduced pressure at 50°C to 16.7% of the homogenate weight, and freeze-dried at -40°C to 2.13 wt% water content to obtain microcapsules containing the compound plant extracts.

[0115] Preparation Example 9:

[0116] The preparation method is as follows: Weigh the raw materials according to the following weight parts: 8.0 parts of 2,6-dimethyl-β-cyclodextrin, 6.4 parts of γ-cyclodextrin, 2.0 parts of plant extract and 0.4 parts of ginger essential oil; the weight ratio of 2,6-dimethyl-β-cyclodextrin to γ-cyclodextrin is 1:0.8;

[0117] The preparation method of the plant extract is as follows:

[0118] (1) Pulverize the arborvitae leaves and Polygonum multiflorum in a weight ratio of 1:1.2, then heat and boil them with water in a weight ratio of 1:20 for the total weight of the arborvitae leaves and Polygonum multiflorum, and then cool them to 25°C.

[0119] (2) Add glucose and acetic acid bacteria SHBCC D11405 to obtain the fermentation material (the amounts of glucose and acetic acid bacteria in the fermentation material are 3.28 wt% and 5.5 × 10⁻⁶ wt%, respectively). 12 The product was obtained by aerobic fermentation at 30℃ for 5 days (air flow rate 0.3L / (min·1L fermentation space)), sterilized, and concentrated under reduced pressure at 55℃ to a weight of twice the total weight of the arborvitae leaves and Polygonum multiflorum.

[0120] (3) Add ethanol to obtain a mixture (the concentration of ethanol in the mixture is 70wt%), heat at 50℃ and stir at 60rpm for 5 hours, filter with a 0.22μm filter membrane, concentrate the filtrate under reduced pressure at 50℃ to 0.15 times the weight of the filtrate, freeze dry at -40℃ to a water content of 1.27wt%, and obtain the plant extract.

[0121] 2,6-Dimethyl-β-cyclodextrin, γ-cyclodextrin, and water (the weight ratio of water to the total weight of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin was 1:55) were heated at 35°C and stirred at 60 rpm for 0.6 hours. Plant extracts and ginger essential oil were added, and the mixture was stirred at 500 rpm for 0.6 hours. Then, the mixture was homogenized under high pressure (100 MPa, 3 cycles, 30 L / min, homogenization temperature 35°C), concentrated under reduced pressure at 50°C to 16.7% of the homogenate weight, and freeze-dried at -40°C to 2.13 wt% water content to obtain microcapsules containing the compound plant extracts.

[0122] Preparation Example 10:

[0123] The preparation method is as follows: Weigh the raw materials according to the following weight parts: 8.0 parts of 2,6-dimethyl-β-cyclodextrin, 6.4 parts of γ-cyclodextrin, 2.0 parts of plant extract and 0.4 parts of ginger essential oil; the weight ratio of 2,6-dimethyl-β-cyclodextrin to γ-cyclodextrin is 1:0.8;

[0124] The preparation method of the plant extract is as follows:

[0125] (1) Pulverize the arborvitae leaves and Polygonum multiflorum in a weight ratio of 1:1.2, then heat and boil them with water in a weight ratio of 1:20 for the total weight of the arborvitae leaves and Polygonum multiflorum, and then cool them to 25°C.

[0126] (2) Add glucose and Listeria innocense ATCC33090 to obtain the fermentation broth (the amounts of glucose and Listeria innocense ATCC33090 in the fermentation broth were 3.28 wt% and 5.5 × 10⁻⁶ wt%, respectively).12 The product was obtained by aerobic fermentation at 30℃ for 5 days (air flow rate 0.3L / (min·1L fermentation space)), sterilized, and concentrated under reduced pressure at 55℃ to a weight of twice the total weight of the arborvitae leaves and Polygonum multiflorum.

[0127] (3) Add ethanol to obtain a mixture (the concentration of ethanol in the mixture is 35wt%), heat at 50℃ and stir at 60rpm for 5 hours, filter with a 0.22μm filter membrane, concentrate the filtrate under reduced pressure at 50℃ to 0.15 times the weight of the filtrate, freeze dry at -40℃ to a water content of 1.27wt%, and obtain the plant extract.

[0128] 2,6-Dimethyl-β-cyclodextrin, γ-cyclodextrin, and water (the weight ratio of water to the total weight of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin was 1:55) were heated at 35°C and stirred at 60 rpm for 0.6 hours. Plant extracts and ginger essential oil were added, and the mixture was stirred at 500 rpm for 0.6 hours. Then, the mixture was homogenized under high pressure (100 MPa, 3 cycles, 30 L / min, homogenization temperature 35°C), concentrated under reduced pressure at 50°C to 16.7% of the homogenate weight, and freeze-dried at -40°C to 2.13 wt% water content to obtain microcapsules containing the compound plant extracts.

[0129] Test: Hair loss prevention and hair regrowth test

[0130] Type II 5α reductase directly participates in the pathological process of androgenetic alopecia and hair follicle atrophy by regulating DHT production. Inhibiting the activity of type II 5α reductase can inhibit hair loss and promote hair regrowth.

[0131] The product from the above preparation example was mixed with 25wt% ethanol aqueous solution at a weight ratio of 2:48, stirred at 30°C and 100rpm for 0.3 hours, and then sterilized to obtain the sample.

[0132] A solution of enzyme was prepared by mixing 5 mL of deionized water, 2 μL of 1 mmol / L testosterone solution, 600 μL of 10 mg / mL rat steroid 5α-reductase SRD5A2 (type II 5α-reductase) solution, and 200 μL of 1 mmol / L NADPH solution. The enzyme solution was incubated at 37°C and 5% CO2 for 40 minutes. 0.5 mL of sample was added to the total enzyme solution to obtain the test solution, which was then incubated at 37°C and 5% CO2 for 40 minutes. The absorbance of the enzyme solution and the test solution before and after incubation were measured at 340 nm using a UV-Vis spectrophotometer. The decrease in absorbance of the test solution before and after incubation for different samples was calculated and denoted as ΔAn (where n is 1-10, corresponding to the sample numbers in Preparation Examples 1-10), and the decrease in absorbance of the enzyme solution before and after incubation was calculated and denoted as ΔA0. The inhibition rate of type II 5α reductase activity in each group was calculated using the following formula: 100% × (△A0 - △An) ÷ △A0. The ratio α of the inhibition rate of type II 5α reductase activity in each group compared to the product of Preparation Example 1 was also calculated. The results are shown in Table 1.

[0133] Table 1: Inhibition rate of type II 5α reductase activity

[0134]

[0135] As shown in Table 1, the microcapsules prepared by this invention can effectively inhibit the activity of type II 5α reductase and have the effect of preventing hair loss and promoting hair growth.

[0136] Combining the test results of Preparation Example 1 and Preparation Example 7, it can be seen that the plant extract prepared by fermentation with Listeria monocytogenes ATCC33090 can effectively enhance the activity of microcapsules inhibiting type II 5α reductase. The use of this fermentation strain can enrich the types and concentrations of active ingredients that inhibit type II 5α reductase activity, giving the microcapsules excellent effects.

[0137] Based on the test results of Preparation Examples 1 and 8-9, it can be concluded that the Listeria monocytogenes ATCC33090 used in this invention has a better effect on promoting hair growth and preventing hair loss compared to other bacteria. The specific fermentation bacteria selected in this invention can effectively enhance the efficacy of microcapsules.

[0138] Based on the test results of Preparation Examples 1 and 10, it can be seen that the high-concentration ethanol extraction method of the present invention can effectively improve the microencapsulation effect, and the effect is even better when the concentration is controlled at 65-80wt%.

[0139] To investigate the effects of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin on the preservation of microcapsules, the products obtained in Preparation Examples 1 and 3-6 were placed at 35°C for 3 months. The inhibition rate against type II 5α-reductase activity was then tested using the same method described above. The results are shown in Table 1. The retention rate (β value) of the inhibition rate against type II 5α-reductase activity after 3 months of storage compared to the state before storage was calculated. The results are shown in Table 2.

[0140] Table 2: Inhibition rate and β value of type II 5α reductase activity after 3 months

[0141]

[0142]

[0143] As can be seen from the test results in Table 2, the microcapsules prepared by this invention have high stability, and the efficiency retention rate of the active ingredients reaches 96.47% after being placed at 35°C for 3 months.

[0144] A comparison of Preparation Examples 1 and 3-4 shows that when only one of 2,6-dimethyl-β-cyclodextrin or γ-cyclodextrin is used for encapsulation to prepare microcapsules, the active ingredient retention efficiency of the microcapsules is low, and the maximum efficacy cannot be achieved. 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin can synergistically enhance the stability of the microcapsules, and synergistically enhance the retention of the drug efficacy against type II 5α-reductase activity inhibition rate. Furthermore, a comparison of Preparation Examples 1 and 5-6 shows that when the weight ratio of 2,6-dimethyl-β-cyclodextrin to γ-cyclodextrin is 1:0.6-0.8, the drug efficacy retention of the microcapsules is better.

Claims

1. A composite plant extract encapsulated in microcapsules, characterized in that, The ingredients include 2,6-dimethyl-β-cyclodextrin, γ-cyclodextrin, plant extracts, and ginger essential oil; The weight ratio of 2,6-dimethyl-β-cyclodextrin to γ-cyclodextrin was 1:0.6-0.8; The preparation method of plant extract includes: (1) crushing arborvitae leaves and Polygonum multiflorum, heating and mixing with water and then cooling; (2) adding glucose and Listeria monocytogenes ATCC33090 to obtain fermentation material, fermenting to obtain fermentation product, sterilizing and concentrating; (3) adding ethanol to obtain a mixture, heating and extracting, filtering, concentrating the filtrate, drying to obtain plant extract. The concentration of ethanol in the mixture is 65-80 wt%.

2. The composite plant extract encapsulated microcapsules according to claim 1, characterized in that, By weight, the ingredients include 5-10 parts of 2,6-dimethyl-β-cyclodextrin, 4-8 parts of γ-cyclodextrin, 0.8-2.5 parts of plant extracts and 0.2-0.5 parts of ginger essential oil.

3. The composite plant extract encapsulated microcapsules according to claim 1, characterized in that, The weight ratio of Platycladus orientalis leaves to Polygonum multiflorum is 1:0.8-1.5; Alternatively, Listeria innocense ATCC33090 can be used in the fermentation substrate at a dosage of 10. 10 Up to 10 13 per g.

4. The composite plant extract encapsulated microcapsules according to claim 1, characterized in that, Fermentation temperature and time are 25-40℃, 3-7 days; Alternatively, the temperature and time for heating and extraction are 40-60℃ for 3-10 hours.

5. A method for preparing microcapsules of composite plant extracts according to any one of claims 1-4, characterized in that, The process includes the following steps: heating and stirring 2,6-dimethyl-β-cyclodextrin, γ-cyclodextrin, and water; adding plant extracts and ginger essential oil, stirring, then homogenizing under high pressure, concentrating, and drying to obtain microcapsules containing the complex plant extracts.

6. The preparation method according to claim 5, characterized in that, The weight ratio of water to the total weight of 2,6-dimethyl-β-cyclodextrin and γ-cyclodextrin is 1:40-150.

7. The use of a composite plant extract encapsulated in microcapsules according to any one of claims 1-4 in the preparation of cosmetics or hair loss prevention and hair growth drugs.

8. The application of a composite plant extract encapsulated in microcapsules according to any one of claims 1-4 in the preparation of hair loss prevention and hair growth essential oil.

Images