A combined detection tube for detecting alcohol concentration in blood

Abstract

The present application belongs to the field of gas detection, and relates to a combined detection tube for detecting alcohol concentration in blood. The detection tube is composed of a sample adding tube and a detection tube. The sample adding tube is internally provided with non-woven fabric, filler and stopper, which can effectively remove red blood cells and absorb moisture when the sample passes through, so that alcohol substances are gasified and diffused into the detection tube under the action of carbonates. The detection tube is further provided with a multi-layer structure including non-woven fabric, filler and different color indicating glue, so that after the oxidation-reduction reaction of alcohol substances and Cr(VI), the alcohol concentration can be directly reflected according to the color change (orange to blue) gradient, and qualitative and quantitative analysis in the range of 0.2-2.0 mg / mL can be realized. The whole detection process takes less than 8 minutes.

Description

Technical Field

[0001] This invention belongs to the field of gas detection and relates to a combined detection tube for detecting alcohol concentration in blood. Background Technology

[0002] Currently, the detection of alcohols in blood mainly relies on large-scale specialized equipment such as gas chromatographs. While this technology boasts high sensitivity and accuracy, it still has several limitations in practical applications. First, the sample pretreatment process is complex and cumbersome, typically requiring multiple steps such as extraction, purification, and concentration. This is not only time-consuming but also demands high operational skills from laboratory personnel, making it susceptible to errors due to human factors. Second, the detection cycle is generally long; from sample collection and transportation to laboratory analysis, it often takes several hours or even longer to obtain the final results.

[0003] Furthermore, the current testing process heavily relies on specialized instruments and equipment in fixed locations, limiting its application in field or on-site environments and hindering rapid response and immediate analysis. Particularly in the field of forensic identification, the complex and ever-changing environment of crime scenes makes it difficult for existing testing methods to quickly obtain physicochemical data on blood alcohols at the scene. This poses significant challenges for forensic workers in determining the nature of the case and assessing the condition of the parties involved. Therefore, there is an urgent need to develop a portable, rapid, and efficient blood alcohol detection technology that can directly introduce physicochemical testing into the crime scene, improving the technical level of on-site investigation. Immediate on-site testing can not only shorten data analysis time but also provide a scientific basis for preliminary case assessment. Summary of the Invention

[0004] The purpose of this invention is to address the aforementioned problems in the existing technology by proposing a combined detection tube for detecting alcohol concentration in blood, which effectively solves the matrix interference problem in direct detection of whole blood and achieves detection accuracy at the level of laboratory instruments.

[0005] The objective of this invention can be achieved through the following technical solutions:

[0006] A combination detection tube for detecting alcohol concentration in blood, the combination detection tube being composed of a sample dispensing tube and a detection tube connected together;

[0007] The sample loading tube includes a glass tube, and from the inlet end of the glass tube to the plugging end, there are a non-woven fabric section, a filler section, and a plugging material section arranged sequentially.

[0008] The detection tube consists of a glass tube, and from the inlet end of the glass tube to the blockage end, there are sequentially arranged a non-woven fabric section, a filler section, a blue indicator adhesive section, an orange indicator adhesive section, another blue indicator adhesive section, and a blockage section.

[0009] Preferably, the detection combination tube is composed of the plugged end of the sample dispensing tube and the inlet end of the detection tube.

[0010] This invention involves drawing whole blood for testing and injecting it into the inlet of a sample tube. As the blood passes through the sample tube, red blood cells are fixed in an alkaline environment, while water in the whole blood is adsorbed and removed. Alcohols vaporize and diffuse into the detection tube under the action of carbonates, where they undergo a redox reaction with Cr(VI).

[0011] CH3CH2OH + 2Cr2O72- + 16H + →2Cr 3+ +3CH3COOH+11H2O,

[0012] The color change gradient (orange → cyan) is linearly correlated with the alcohol concentration. With the use of a longer detection tube and the instrument's matching detection function, qualitative and quantitative detection of 0.2-2.0 mg / mL can be achieved, with a detection time of <8 min.

[0013] In the aforementioned combination detection tube for detecting alcohol concentration in blood, the packing material section has a length of 80-110 mm and an average particle size of 0.3-0.5 mm. The preparation method of the packing material includes the following steps:

[0014] S1. Mix silica particles with an alkaline solution and then dry at high temperature to obtain a porous alkaline carrier;

[0015] S2. Mix silica particles with carbonate solution and then dry at high temperature to obtain carbonate-modified carrier;

[0016] S3. Finally, the porous basic support and the carbonate-modified support are mixed.

[0017] In the aforementioned combined detection tube for detecting alcohol concentration in blood, the mass ratio of porous basic carrier to carbonate-modified carrier is (2-5):1.

[0018] The packing material used in this invention plays a crucial role in the detection of alcohol concentration in blood. The packing material has a section length controlled at 80-110 mm and an average particle size of 0.3-0.5 mm, possessing a suitable specific surface area and good permeability, effectively ensuring the uniform flow of sample gas within the detection tube and the sufficiency of the reaction. Its preparation process consists of three steps: First, silica particles are mixed with an alkaline solution and dried at high temperature to form an alkaline carrier with a porous structure. This carrier can fix red blood cells during sample addition and simultaneously promote the release of alcohols from the blood. Second, silica particles are mixed with a carbonate solution and dried to obtain a carbonate-modified carrier, which decomposes under heating conditions to produce carbon dioxide gas, helping to create a local alkaline environment and further promoting the vaporization and escape of alcohols. Finally, the two carriers are mixed at a mass ratio of (2-5):1 to form a composite packing system with synergistic effects. This graded design not only enhances the packing material's adaptability to complex matrix compositions but also effectively removes some impurities and interfering gases, such as sulfides and aldehydes, thereby reducing the interference of non-target substances on subsequent colorimetric reactions. Through this optimized combination of structure and function, the packing material can achieve efficient extraction and purification of alcohols in whole blood samples in a short time, allowing most alcohol gases to rapidly evaporate and enter the detection tube, significantly improving detection sensitivity and accuracy. Furthermore, the packing material possesses good stability and reusability, helping to reduce detection costs and improve the practicality of on-site testing.

[0019] In the aforementioned combination test tube for detecting alcohol concentration in blood, the alkaline solution includes at least one of sodium hydroxide, potassium hydroxide, and calcium hydroxide.

[0020] In the aforementioned combination test tube for detecting alcohol concentration in blood, the carbonate includes at least one of sodium carbonate, potassium carbonate, and calcium carbonate.

[0021] In the aforementioned combination test tube for detecting alcohol concentration in blood, the high-temperature drying temperature is 150-250℃, and the time is 35-45 minutes.

[0022] In the aforementioned combination detection tube for detecting alcohol concentration in blood, the length of the orange indicator gel segment is 50-65 mm, and the preparation method of the orange indicator gel includes the following steps:

[0023] S1. Mix high borosilicate glass powder and potassium dichromate solution, and then dry at high temperature to obtain the initial version of the indicator adhesive;

[0024] S2. Add concentrated sulfuric acid to the initial version of the indicator gel and mix to obtain orange indicator gel.

[0025] In the aforementioned combination detection tube for detecting alcohol concentration in blood, the mass ratio of borosilicate glass powder to potassium dichromate solution is (25-35):1, wherein the concentration of potassium dichromate solution is 0.2-0.5 mol / L.

[0026] In the aforementioned combination test tube for detecting alcohol concentration in blood, the blue indicator gel is blue glass powder, and the length of the blue indicator gel segment is 2-3 mm.

[0027] In the aforementioned combination test tube for detecting alcohol concentration in blood, the high-temperature drying temperature is 100-150℃, and the time is 25-35 minutes.

[0028] The beneficial effects of this invention are as follows:

[0029] 1. This invention proposes a combined detection tube for detecting alcohol concentrations in blood. Its ingenious design solves the common matrix interference problem in direct whole blood testing, achieving detection accuracy comparable to laboratory instruments. The detection tube consists of two parts: a sample loading tube and a detection tube. The sample loading tube contains non-woven fabric, filler, and a plug to effectively remove red blood cells and absorb moisture as the sample passes through, allowing alcohols to vaporize and diffuse into the detection tube under the action of carbonates. The detection tube further incorporates a multi-layered structure including non-woven fabric, filler, and different colored indicator adhesives. This allows the alcohol concentration to be visually reflected by the color change (orange → cyan) gradient after the redox reaction between alcohols and Cr(VI), thus enabling qualitative and quantitative analysis within the range of 0.2-2.0 mg / mL. The entire detection process takes less than 8 minutes.

[0030] 2. The filler material of this invention is designed using a graded preparation method. First, silica particles are mixed with an alkaline solution or a carbonate solution, and then dried at high temperature to obtain a porous alkaline carrier and a carbonate-modified carrier. The two are mixed in a specific ratio to enhance the effectiveness and specificity of sample processing.

[0031] 3. The preparation of the orange indicator adhesive of this invention is also carefully designed. It is prepared by mixing high borosilicate glass powder with potassium dichromate solution, drying under specific conditions, and then adding concentrated sulfuric acid to ensure the sensitivity and accuracy of the reaction. The blue indicator adhesive, as another important component, not only serves to differentiate and identify the components but also participates in monitoring the progress of the chemical reaction.

[0032] 4. With its unique design concept and simple operation, this invention greatly simplifies the detection process of alcohols in whole blood, shortens the detection time, and ensures the accuracy of the results. It is very suitable for on-site rapid detection applications and provides strong data support and technical guarantee for research and practice in related fields. Attached Figure Description

[0033] Figure 1 Schematic diagram of sample addition tube for Example 1; 1-1, high borosilicate glass tube; 1-2, medical non-woven fabric; 1-3, packing material; 1-4, medical-grade plug;

[0034] Figure 2 Schematic diagram of the detection tube in Example 1; 2-1, high borosilicate glass tube; 2-2, medical non-woven fabric; 2-3, filler; 2-4, blue indicator adhesive; 2-5, orange indicator adhesive; 2-6, medical-grade plug.

[0035] Figure 3 This is a schematic diagram of the actual testing of the sample tube and test tube in Example 1.

[0036] Figure 4 The image shows the sample tube and test tube of Example 1. Detailed Implementation

[0037] The technical solution of the present invention will be further described and illustrated below with reference to specific embodiments and accompanying drawings. It should be understood that the specific embodiments described herein are only for the purpose of helping to understand the present invention and are not intended to limit the specific scope of the present invention. Furthermore, the accompanying drawings used herein are merely for better illustrating the content disclosed in the present invention and do not limit the scope of protection. Unless otherwise specified, the raw materials used in the embodiments of the present invention are all commonly used in the art, and the methods used in the embodiments are all conventional methods in the art.

[0038] Example 1:

[0039] A physical image as follows Figure 4 A combination detection tube for detecting alcohol concentrations in blood, the combination detection tube consisting of a sample dispensing tube and a detection tube connected together;

[0040] Among them, the sample tube is like Figure 1 The diagram shows a glass tube, from left to right, consisting of a non-woven fabric section, a filler section, and a plug section.

[0041] Detection tube such as Figure 2 The device includes a glass tube, from left to right, consisting of a non-woven fabric section, a filler section, a blue indicator adhesive section, an orange indicator adhesive section, another blue indicator adhesive section, and a blockage section.

[0042] The glass tubes for the sample addition tube and the test tube are single-port high borosilicate glass tubes with an inner diameter of 0.32 mm and a length of 145 mm.

[0043] The sample loading tube has a packing section length of 100 mm, and the test tube has a packing section length of 20 mm. The average particle size of the packing is 0.425 mm. The packing preparation method includes the following steps:

[0044] S1. Mix 40-mesh silica particles with a 30wt% potassium hydroxide solution for 20 min, and then dry them at 200℃ for 40 min to obtain a porous alkaline support.

[0045] S2. Mix 40-mesh silica particles with a 50wt% potassium carbonate solution for 20 min, and then dry them at 200℃ for 40 min to obtain a carbonate-modified support.

[0046] S3. Finally, the porous alkaline carrier and the carbonate-modified carrier are mixed at a mass ratio of 3:1, and then vibrated and sieved until the particle size is uniform. The mixture is then injected into the pretreated glass tube using a vacuum filling method, with the filler length controlled to be 100 mm.

[0047] The orange indicator gel segment is 57 mm long. The preparation method of the orange indicator gel includes the following steps:

[0048] S1. Mix 80-mesh high borosilicate glass powder and 0.34 mol / L potassium dichromate solution at a mass ratio of 30:1 for 30 min, and then dry at 130℃ for 30 min to obtain the initial version of the indicator adhesive.

[0049] S2. Mix the initial indicator gel and 98% sulfuric acid at a mass ratio of 375:2 for 30 minutes, and then let it stand for 12 hours to obtain orange indicator gel.

[0050] The blue indicator adhesive is made of blue glass powder, and the length of the blue indicator adhesive segment is 2.5mm.

[0051] The blockage section uses medical-grade rubber plugs.

[0052] Example 2:

[0053] The only difference from Example 1 is that the packing material is a porous alkaline carrier.

[0054] Example 3:

[0055] The only difference from Example 1 is that the filler is a carbonate-modified carrier.

[0056] Example 4:

[0057] The only difference from Example 1 is that the length of the packing sample tube is only 50 mm.

[0058] Example 5:

[0059] The only difference from Example 1 is that the length of the packing sample tube is only 135 mm.

[0060] Example 6:

[0061] The only difference from Example 1 is that the orange indicator gel is the initial version of the indicator gel.

[0062] Comparative Example 1:

[0063] The only difference from Example 1 is that the combined detection tube does not contain a sample addition tube.

[0064] like Figure 3 The sample tube and the detection tube shown are connected by a rubber tube. Whole blood samples from Examples 1-6 and Comparative Example 1 are directly injected into the sample tube and the alcohols are separated and detected through the detection tube.

[0065] Table 1: Performance tests of the test tubes prepared in Examples 1-6 and Comparative Example 1.

[0066]

[0067]

[0068] As can be seen from the table, Example 1 performed best, with an alcohol release rate of 90% and a detection sensitivity range of 0.2-2.0 mg / mL, demonstrating good release capacity and detection accuracy. This indicates that its packing structure, length, and indicator gel design were reasonable, effectively releasing alcohols from the blood and achieving accurate detection. In contrast, Examples 2 and 3 used only a single type of packing material (porous alkaline carrier or carbonate-modified carrier, respectively), resulting in a release rate of only 70% and a significantly wider detection sensitivity range (0.5-3.0 mg / mL), indicating that a single packing material cannot achieve the synergistic enhancement effect of a composite packing material. In Example 4, shortening the packing section length of the sample tube to 50 mm further reduced the release rate to 50% and the sensitivity to 0.7-3.5 mg / mL, indicating that insufficient packing length affects the extraction efficiency of alcohols. While Example 5 extended the packing section to 135 mm, achieving a 100% release rate, the excessive packing length caused excessive airflow resistance, ultimately preventing detection. This demonstrates that there is an optimal range for packing length. Example 6 used an untreated initial version of orange indicator gel. Although the release rate was 90%, the detection sensitivity was 0, indicating that the color reaction mechanism could not be effectively established and was not chemically feasible. Comparative Example 1 did not use a sample addition tube and directly introduced whole blood samples into the detection tube. The results showed that both the release rate and sensitivity were 0, verifying the necessity of the sample addition tube in the process of removing red blood cells and releasing alcohols.

[0069] In summary, this invention proposes a combined detection tube for detecting alcohol concentrations in blood. Its ingenious design solves the common matrix interference problem in direct whole blood testing, achieving detection accuracy comparable to laboratory instruments. The detection tube consists of a sample loading tube and a detection tube. The sample loading tube contains non-woven fabric, filler, and a plug to effectively remove red blood cells and absorb moisture as the sample passes through, allowing alcohols to vaporize and diffuse into the detection tube under the action of carbonates. The detection tube further incorporates a multi-layered structure including non-woven fabric, filler, and different colored indicator adhesives. This allows the alcohol concentration to be visually reflected by the length of the color change (orange → cyan) after the alcohol undergoes a redox reaction with Cr(VI), thus enabling qualitative and quantitative analysis within the range of 0.2-2.0 mg / mL. The entire detection process takes less than 8 minutes.

[0070] The embodiments described herein cover any points not exhaustively within the scope of the technical claims of this invention, as well as new technical solutions formed by equivalent substitutions of one or more technical features in the embodiments. These are all within the scope of the claims of this invention. Furthermore, in all listed or unlisted embodiments of this invention, each parameter in the same embodiment merely represents an instance (i.e., a feasible solution) of its technical solution, and there is no strict coordination or limitation relationship between the parameters. The parameters can be substituted for each other without violating axioms and the claims of this invention, unless otherwise stated.

[0071] The technical means disclosed in this invention are not limited to those described above, but also include technical solutions composed of any combination of the above technical features. The above descriptions are specific embodiments of this invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of this invention, and these improvements and modifications are also considered within the scope of protection of this invention.

[0072] The specific embodiments described herein are merely illustrative of the spirit of the invention. Those skilled in the art to which this invention pertains may make various modifications or additions to the described specific embodiments or use similar methods to substitute them, without departing from the spirit of the invention or exceeding the scope defined by the appended claims.

Claims

1. A combination detection tube for detecting alcohol concentration in blood, characterized in that, The combined detection tube is composed of a sample dispensing tube and a detection tube connected together; The sample loading tube includes a glass tube, and from the outlet end of the glass tube to the blockage end, there are a non-woven fabric section, a filler section, and a blockage section arranged in sequence. The detection tube includes a glass tube, and from the outlet end of the glass tube to the blockage end, there are sequentially arranged a non-woven fabric section, a filler section, a blue indicator adhesive section, an orange indicator adhesive section, another blue indicator adhesive section, and a blockage section; The packing section has a length of 80-110 mm and an average particle size of 0.3-0.5 mm. The preparation method of the packing includes the following steps: S1. Mix silica particles with an alkaline solution and then dry at high temperature to obtain a porous alkaline carrier; S2. Mix silica particles with carbonate solution and then dry at high temperature to obtain carbonate-modified carrier; S3. Finally, the porous basic support and the carbonate-modified support are mixed. The orange indicator gel segment is 50-65 mm in length. The preparation method of the orange indicator gel includes the following steps: S1. Mix high borosilicate glass powder and potassium dichromate solution, and then dry at high temperature to obtain the initial version of the indicator adhesive; S2. Add concentrated sulfuric acid to the initial version of the indicator gel and mix to obtain orange indicator gel.

2. The combined detection tube for detecting alcohol concentration in blood according to claim 1, characterized in that, The mass ratio of the porous basic support to the carbonate-modified support is (2-5):

1.

3. A combined detection tube for detecting alcohol concentration in blood according to claim 1, characterized in that, Alkaline solutions include at least one of sodium hydroxide, potassium hydroxide, and calcium hydroxide.

4. A combined detection tube for detecting alcohol concentration in blood according to claim 1, characterized in that, Carbonates include at least one of sodium carbonate, potassium carbonate, and calcium carbonate.

5. A combined detection tube for detecting alcohol concentration in blood according to claim 1, characterized in that, During the preparation of the filler, the high-temperature drying temperature is 150-250℃ and the time is 35-45min.

6. A combined detection tube for detecting alcohol concentration in blood according to claim 1, characterized in that, The mass ratio of high borosilicate glass powder to potassium dichromate solution is (25-35):1, wherein the concentration of potassium dichromate solution is 0.2-0.5 mol / L.

7. A combined detection tube for detecting alcohol concentration in blood according to claim 1, characterized in that, The blue indicator adhesive is made of blue glass powder, and the length of the blue indicator adhesive segment is 2-3mm.

8. A combined detection tube for detecting alcohol concentration in blood according to claim 1, characterized in that, In the preparation of the orange indicator adhesive, the high-temperature drying temperature is 100-150℃ and the time is 25-35 minutes.

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