Fixing method and device for mouse tail intravenous injection

Abstract

The invention relates to the technical field of medical instruments, in particular to a mouse tail intravenous injection fixing method and device which comprises a supporting panel, an axial pressing mechanism, an open barrel, two opening locking mechanisms, four circumferential pressing mechanisms and an overall inclination angle adjustable supporting mechanism. The axial pressing mechanism comprises a lead screw, a supporting plate, two first locking nuts, a pressing plate, two second locking nuts and a downward pressing arc plate. According to the device, the mouse is axially and effectively fixed through the adjustable lead screw, the pressing plate, the supporting plate with the U-shaped opening and the axial pressing mechanism. A transparent and elastic open type cylinder body is matched with four circumferential pressing mechanisms, so that mice with different body types can easily enter and be circumferentially fixed, ventilation and comfort are guaranteed, and experimental observation is facilitated; through the overall inclination angle adjustable supporting mechanism, the comfort of an operator is improved, and the accuracy and efficiency of injection are improved; the device is convenient to adjust, stress response and accidental injury of mice can be effectively reduced, and the caudal vein injection efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a fixation method and device for mouse tail vein injection. Background Art

[0002] Mouse tail vein injection holders are widely used in many research settings, including medical research institutions of all sizes, biological research centers, animal quarantine centers, and university laboratories. Currently, the use of experimental mice in exploring gene function, disease causes, and pathogenesis is widespread and holds great promise. Tail vein injection in mice is simple and easy to perform, both for injection and blood collection. Furthermore, the superficial and fixed location of the tail vein facilitates stable and accurate injections. Intravenous injection offers rapid drug absorption, the highest utilization rate, and significant efficacy, making it suitable for experiments requiring precise control of drug dosage and rate. Tail vein injection is widely used in a variety of experiments, including pharmacology and pharmacokinetic studies, ensuring rapid drug distribution and supporting subsequent immune response studies. Due to the small size of mice, their sensitivity to external stimuli, and their timidity, coupled with the thin tail vein, optimizing the method and device for securing the mouse and its tail during tail vein injection is crucial.

[0003] Traditional mouse and tail holders have a cylindrical shape with an opening that can be opened and closed at one end and a breathable opening at the other end. When performing tail vein injections, the mouse is placed in the cylindrical holder, and then one end of the cylinder is closed. The opening at the other end is then used to expose the mouse's tail for tail vein injection. During tail vein injections, the mouse needs to be fixed to maintain stability and facilitate tail vein injections. Currently, mice are fixed by squeezing, but the squeezing force and squeezing space are not adjustable, making it difficult to adapt to the fixation of mice of different diameters and lengths, which can easily cause mice to be injured; it is difficult to allow mice to enter, and if brute force is used to drag them, it can also easily cause mice to be injured; and the mouse accommodation space is small and the design is unreasonable, which can easily cause stress reactions in experimental mice; the overall height and inclination angle cannot be adjusted, which can easily cause discomfort to the experimenter and is not convenient for fast and accurate injections. Summary of the Invention

[0004] The object of the present invention is to provide a method and device for fixation of mice for tail vein injection, so as to solve the problems in the prior art of using squeezing to fix mice, but the squeezing force and squeezing space are not adjustable, which makes it difficult to adapt to the fixation of mice of different diameters and lengths, and easily causes injury to the mice; it is difficult to allow the mice to enter, and if brute force is used to drag them, it is also easy to cause injury to the mice; the mouse accommodation space is small and the design is unreasonable, which easily causes stress reactions in experimental mice; the overall height and inclination angle are not adjustable, which easily causes discomfort to the experimenter and is not convenient for rapid and accurate injection.

[0005] To achieve the above-mentioned objectives, the present invention provides a fixation method and device for mouse tail vein injection, comprising a support panel, an axial clamping mechanism, an open cylinder, two open locking mechanisms, four circumferential clamping mechanisms and an overall inclination-adjustable support mechanism, wherein the axial clamping mechanism comprises a screw, a support plate, two first locking nuts, a pressure plate, two second locking nuts and a lower pressure arc plate, the open cylinder is arranged on the support panel, the support plate is a U-shaped opening, the support plate is arranged on the support panel and is located on the left side of the open cylinder, the screw is placed on the support plate, and the right end of the screw is located inside the open cylinder, the pressure plate is sleeved on the outside of the screw, the two second locking nuts are respectively adapted to the screw and are respectively located on both sides of the pressure plate, the lower pressure arc plate is fixedly connected to the right end of the pressure plate, the two first locking nuts are respectively adapted to the screw and are respectively located on both sides of the support plate.

[0006] Among them, each of the opening locking mechanisms includes two U-shaped opening plates, two third locking nuts and a first screw, each of the circumferential pressing mechanisms includes a second screw and a first fixing plate, the two U-shaped opening plates are symmetrically arranged at the upper end of the open cylinder, the first screw is placed on the two U-shaped opening plates, the two third locking nuts are respectively adapted to the first screws, and the two third locking nuts are respectively against the side surfaces of the U-shaped opening plate.

[0007] Among them, the overall inclination adjustable support mechanism includes a second fixed plate, a waist-shaped fixed plate, a movable plate and a locking screw, the waist-shaped fixed plate has a waist-shaped hole, the second fixed plate is fixedly connected to the lower end of the support panel, the waist-shaped fixed plate is fixedly connected to the lower end of the support panel, and is located on the left side of the second fixed plate, one end of the locking screw passes through the waist-shaped hole and is adapted to the movable plate.

[0008] Among them, the fixing device for tail vein injection of mice also includes a tail clamping mechanism, a tail L-shaped blocking mechanism, an axial adjustment locking mechanism and a yellow light illumination system, the tail clamping mechanism includes a U-shaped plate, a pressure block, two guide mechanisms, a vertical lifting rod and a tail placement plate, the pressure block includes an inverted V-shaped clamping plate and a fixed block, the fixed block has sliding grooves on both sides, the middle of the fixed block has a thread, the tail placement plate has a V-shaped groove, the axial adjustment locking mechanism is arranged at the upper end of the support panel, the tail placement plate is arranged at the upper end of the axial adjustment locking mechanism, the U-shaped plate is fixedly arranged at the upper end of the tail placement plate, the two guide mechanisms are symmetrically arranged on the U-shaped plate, the pressure block is located between the two guide mechanisms, the two guide mechanisms are slidably adapted to the corresponding sliding grooves, the vertical lifting rod passes through the U-shaped plate and is adapted to the thread.

[0009] Among them, the tail L-shaped blocking mechanism includes an L-shaped tail blocking plate, a first locking bolt and a fourth locking nut, the L-shaped tail blocking plate has a U-shaped opening, the L-shaped tail blocking plate is arranged at the upper end of the support panel, the first locking bolt passes through the support panel and the L-shaped tail blocking plate, the fourth locking nut is arranged on the first locking bolt, the fourth locking nut is against the L-shaped tail blocking plate, and the lower end of the inverted V-shaped clamping plate has a rubber pad.

[0010] In which, the axial adjustment locking mechanism includes two L-shaped plates, two second locking bolts, and two fifth locking nuts. The support panel has two strip-shaped slots. The two L-shaped plates are respectively placed on the upper ends of the support panel. The two second locking bolts pass through the corresponding strip-shaped slots respectively. The two fifth locking nuts are respectively adapted to the corresponding second locking bolts, and the two fifth locking nuts are abutted against the corresponding L-shaped plates. The tail placement plate is fixedly arranged on the upper ends of the two L-shaped plates, and the L-shaped tail blocking plate is located between the two L-shaped plates.

[0011] Wherein, the yellow light lighting system includes a bulb and a generator switch, the bulb is arranged at the lower end of the V-shaped groove, and the generator switch is arranged at the lower end of the V-shaped groove.

[0012] The present invention also provides a method for fixing a mouse for tail vein injection, using the above-mentioned fixing device for mouse tail vein injection, comprising the following steps: In the first step, the locking screw is loosened, and the movable plate is raised to ensure that the supporting panel is in a horizontal state; Step 2: Fix the L-shaped tail blocking plate to the support panel by tightening the first locking bolt and the fourth locking nut; Step 3: Tighten the second locking bolt and the fifth locking nut by axially adjusting the locking mechanism to preliminarily fix the tail clamping mechanism and the yellow light lighting system to the support panel; Step 4: Fix the pressing plate to the screw rod using the two first locking nuts according to the length and shape of the mouse, and preliminarily adjust the positions of the two second locking nuts on the screw rod; Step 5: Install the mouse head into the open cylinder with the tail on the left and the right side on the right side. The right side of the open cylinder should be placed on the L-shaped blocking mechanism at the tail, and the bottom should be placed flat on the support panel. Then push the screw and the right pressure plate into the open cylinder, and put the left side of the screw into the U-shaped groove of the support panel. Step 6: Finally, observe the condition of the mouse and adjust the two opening locking mechanisms and the four circumferential pressing mechanisms in sequence to comfortably position the mouse in the open cylinder 5, and then fix the open cylinder 5 to the support panel; Step 7: Determine the axial position of the tail clamping mechanism based on the tail vein injection location and axially adjust and tighten the two second locking bolts and the two fifth nuts to achieve locking. Then, rotate the vertical lifting rod and, under the guidance of the guide mechanism, drive the clamping block downward without rotating, thereby comfortably clamping the mouse's tail. In the eighth step, the left side of the support panel is raised by lowering the movable plate, thereby tilting the entire device downward to a suitable position for intravenous injection. Finally, after soaking the mouse's tail with hot water, the light is turned on to clearly display the distribution of the mouse's tail veins, and the injection is ready. Then, turn on the light bulb through the generator switch, and place the mouse in a lateral position to find the lateral tail vein. If the lateral tail vein is not clear, find the dorsal tail vein. Under the illumination of the yellow light, the tail vein will be clearly presented. Generally, find the lateral tail vein first. If the lateral tail vein is not clear, find the dorsal tail vein.

[0013] After finding the puncture point, use alcohol to disinfect the injection site, lock the axial position of the clamping mechanism, and press down the clamp to further expand the blood vessels. Then, pinch the tip of the mouse's tail with one hand to prevent it from swinging due to the mouse's struggle during the injection; Hold the syringe with your other hand, with the eye of the needle facing upwards. Place the entire needle against the tail and slowly insert it parallel to the tail vein. Generally, the tail vein can be punctured when the needle is about 1-1.5 mm deep. After successful puncture, push injection can be performed. If the injection volume is less than 100 μL, it can be injected quickly to prevent the mouse from struggling and getting out of the needle. If the injection volume is large, the flow rate must be controlled and not too fast. After the injection is completed, wait a few seconds before withdrawing the needle. Withdraw the needle quickly and then immediately use hemostatic clips or other methods to stop bleeding. After the injection is completed, turn off the lights, loosen the tail clamping mechanism, remove the two opening locking mechanisms and the axial clamping screw, take out the mouse, and complete the entire intravenous injection of the mouse.

[0014] The present invention provides a fixation device for tail vein injection of mice. The mouse is placed in the open cylinder, and the mouse is confined to a local area by the pressure plate. The head of the mouse can be pressed down by the downward pressure arc plate, which can further prevent the mouse from shaking in the open cylinder, keep quiet, and avoid stress response. By locking or loosening the two second locking nuts, different types of pressure plates can be replaced to provide a comfortable environment for mice of different lengths. The axial and circumferential compression of the device are well adjustable, which can well ensure the stability, straightness and comfort of the bodies of mice of different diameters and lengths, and avoid discomfort, stress response or injury to mice that may be caused by traditional devices. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art.

[0016] Figure 1 It is a schematic structural diagram of the entire invention.

[0017] Figure 2 It is a side view of the entire present invention.

[0018] Figure 3 It is a right side view of the entire present invention.

[0019] Figure 4 It is a partial cross-sectional view of the present invention.

[0020] Figure 5 It is a structural schematic diagram of the tail L-shaped blocking mechanism of the present invention.

[0021] Figure 6 It is a schematic structural diagram of the compact of the present invention.

[0022] Figure 7 It is a top view of the compact of the present invention.

[0023] 1-Support panel, 2-Open cylinder, 3-Screw, 4-Support plate, 5-First locking nut, 6-Pressure plate, 7-Second locking nut, 8-Lower pressure arc plate, 9-U-shaped opening plate, 10-Third locking nut, 11-First screw, 12-Second screw, 13-First fixing plate, 14-Second fixing plate, 15-Waist-shaped fixing plate, 16-Movable plate, 17-Locking screw, 18-Waist-shaped hole, 19-U-shaped plate, 20-Pressure block, 21- Guide mechanism, 22-vertical lifting rod, 23-tail placement plate, 24-inverted V-shaped clamping plate, 25-fixed block, 26-slide, 27-thread, 28-V-shaped groove, 29-L-shaped tail blocking plate, 30-first locking bolt, 31-fourth locking nut, 32-U-shaped opening, 33-rubber pad, 34-L-shaped plate, 35-second locking bolt, 36-fifth locking nut, 37-bar slide, 38-bulb, 39-generator switch. DETAILED DESCRIPTION

[0024] The embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, but should not be understood as limiting the present invention.

[0025] See also Figures 1 to 7 ,in. Figure 1 It is a schematic diagram of the overall structure of the present invention. Figure 2 is a side view of the entirety of the present invention, Figure 3 It is the right side view of the whole of the present invention, Figure 4 It is a partial cross-sectional view of the present invention, Figure 5 This is a schematic diagram of the structure of the tail L-shaped blocking mechanism of the present invention. Figure 6 2 is a schematic structural diagram of the briquette 20 of the present invention, Figure 7 FIG. 2 is a top view of a compact 20 according to the present invention.

[0026] The present invention provides a fixing device for mouse tail vein injection, comprising a support panel 1, an axial clamping mechanism, an open cylinder 2, two open locking mechanisms, four circumferential clamping mechanisms and an overall inclination adjustable support mechanism, wherein the axial clamping mechanism comprises a screw 3, a support plate 4, two first locking nuts 5, a pressure plate 6, two second locking nuts 7 and a lower pressure arc plate 8, the open cylinder 2 is arranged on the support panel 1, the support plate 4 is a U-shaped opening 32, the support plate 4 is arranged on the support panel 1 and is located on the left side of the open cylinder 2, the screw 3 is placed on the support plate 4, and the right end of the screw 3 is located inside the open cylinder 2, the pressure plate 6 is sleeved on the outside of the screw 3, the two second locking nuts 7 are respectively adapted to the screw 3, and are respectively located on both sides of the pressure plate 6, the lower pressure arc plate 8 is fixedly connected to the right end of the pressure plate 6, the two first locking nuts 5 are respectively adapted to the screw 3, and are respectively located on both sides of the support plate 4.

[0027] In this embodiment, a mouse is placed in the open cylinder 2, and the mouse is confined to a local area by the pressure plate 6, and the head of the mouse can be pressed down by the downward pressure arc plate 8, which can further prevent the mouse from shaking in the open cylinder 2, keeping quiet, and avoiding stress response. By locking or loosening the two second locking nuts 7, different types of pressure plates 6 can be replaced to provide a comfortable environment for mice of different lengths. The axial and circumferential compression of the device is well adjustable, which can well ensure the stability, straightness and comfort of the mouse bodies of different diameters and lengths, and avoid discomfort, stress response or injury to mice that may be caused by traditional devices.

[0028] Furthermore, each of the opening locking mechanisms includes two U-shaped opening plates 9, two third locking nuts 10 and a first screw 11, and each of the circumferential pressing mechanisms includes a second screw 12 and a first fixing plate 13. The two U-shaped opening plates 9 are symmetrically arranged at the upper ends of the open cylinder 2, and the first screw 11 is placed on the two U-shaped opening plates 9. The two third locking nuts 10 are respectively adapted to the first screws 11, and the two third locking nuts 10 are respectively abutted against the side surfaces of the U-shaped opening 32.

[0029] In this embodiment, the open cylinder 2 can quickly and easily load mice of different diameters from left to right, while facilitating the free breathing of mice in the cylinder; secondly, through the setting of the two opening locking mechanisms, through the cooperation of the two third locking nuts 10 and the first screw 11, the diameter of the open cylinder 2 can be adjusted and fixed, which can adapt to mice of different diameters and prevent them from turning around or shaking inside, while not being pressed too tightly to produce a stress response; the screwing in and out of the second screw 12 adjusted by the four circumferential pressing mechanisms can realize the overall fixation and loosening of the open cylinder 2, facilitate the implementation of the fixation and removal operations of the mouse, and is also conducive to determining the position of the cylinder on the fixing plate; and the use of transparent and elastic materials is conducive to observing the situation of the mouse in the cylinder, can adapt to mice of different diameters, and repeatedly load or remove and complete the experiment.

[0030] Furthermore, the overall inclination-adjustable support mechanism includes a second fixed plate 14, a waist-shaped fixed plate 15, a movable plate 16 and a locking screw 17. The waist-shaped fixed plate 15 has a waist-shaped hole 18. The second fixed plate 14 is fixedly connected to the lower end of the support panel 1. The waist-shaped fixed plate 15 is fixedly connected to the lower end of the support panel 1 and is located on the left side of the second fixed plate 14. One end of the locking screw 17 passes through the waist-shaped hole 18 and is adapted to the movable plate 16.

[0031] In this embodiment, the movable plate 16 can be raised and lowered by setting the waist-shaped hole 18. When it is raised and lowered to the appropriate position, it is locked by the locking screw 17, thereby realizing the raising and lowering adjustment of the height of the left side of the support panel 1, thereby realizing the overall inclination angle adjustment in the range of 20°-30°, which is conducive to easy intravenous injection of mice.

[0032] Furthermore, the fixation device for mouse tail vein injection also includes a tail clamping mechanism, a tail L-shaped blocking mechanism, an axial adjustment locking mechanism and a yellow light illumination system. The tail clamping mechanism includes a U-shaped plate 19, a pressing block 20, two guide mechanisms 21, a vertical lifting rod 22 and a tail placement plate 23. The pressing block 20 includes an inverted V-shaped clamping plate 24 and a fixing block 25. The fixing block 25 has a slide groove 26 on both sides, a thread 27 in the middle of the fixing block 25, and the tail placement plate 23 has a V-shaped groove 28. The axial adjustment locking mechanism is arranged at the upper end of the support panel 1, the tail placement plate 23 is arranged at the upper end of the axial adjustment locking mechanism, the U-shaped plate 19 is fixedly arranged at the upper end of the tail placement plate 23, the two guide mechanisms 21 are symmetrically arranged on the U-shaped plate 19, and the pressure block 20 is located between the two guide mechanisms 21. The two guide mechanisms 21 are slidably adapted to the corresponding slide grooves 26, and the vertical lifting rod 22 passes through the U-shaped plate 19 and is adapted to the thread 27.

[0033] In this embodiment, the vertical lifting rod 22 is rotated to cooperate with the lifting rotating rod and the thread 27, and with the cooperation of the two guide mechanisms 21 and the corresponding slide grooves 26, the pressing block 20 is limited so that the pressing block 20 only rises or falls but does not rotate, which is beneficial to the tightening or loosening of the mouse tail, preventing the mouse tail from shaking, facilitating subsequent experiments, and not making the mouse uncomfortable.

[0034] Furthermore, the tail L-shaped blocking mechanism includes an L-shaped tail blocking plate 29, a first locking bolt 30 and a fourth locking nut 31, the L-shaped tail blocking plate 29 has a U-shaped opening 32, the L-shaped tail blocking plate 29 is arranged at the upper end of the support panel 1, the first locking bolt 30 passes through the support panel 1 and the L-shaped tail blocking plate 29, the fourth locking nut 31 is arranged on the first locking bolt 30, the fourth locking nut 31 is abutted against the L-shaped tail blocking plate 29, and the lower end of the inverted V-shaped clamping plate 24 has a rubber pad 33.

[0035] In this embodiment, when in use, the device is placed at the rear end of the cylindrical fixture, and the tail of the mouse is supported by the L-shaped tail blocking plate 29 to prevent the mouse from moving in the cylindrical fixture. After the mouse is fixed, the tail of the mouse can be pulled out of the U-shaped opening 32 and then placed on the tail placement plate 23 to facilitate intravenous injection. The L-shaped tail blocking plate 29 has an arc-shaped protrusion, which can better fix the mouse, and the L-shaped tail blocking plate 29 is fixed to the support panel 1 through the cooperation of the first locking bolt 30 and the fourth nut.

[0036] Furthermore, the axial adjustment locking mechanism includes two L-shaped plates 34, two second locking bolts 35, and two fifth locking nuts 36. The support panel 1 has two strip-shaped slots 36. The two L-shaped plates 34 are respectively placed on the upper end of the support panel 1. The two second locking bolts 35 pass through the corresponding strip-shaped slots 36 respectively. The two fifth locking nuts 36 are respectively adapted to the corresponding second locking bolts 35, and the two fifth locking nuts 36 are abutted against the corresponding L-shaped plates 34. The tail placement plate 23 is fixedly arranged on the upper ends of the two L-shaped plates 34, and the L-shaped tail blocking plate 29 is located between the two L-shaped plates 34.

[0037] In this embodiment, by screwing the fifth locking nut 36 and the second locking bolt 3518, the limiting fixation of the two L-shaped plates 34 can be cancelled, and axial adjustment can be performed. By changing the pressing position of the mouse tail along the axial adjustment, it is convenient to pull out the mouse tail and select the required vein position for injection.

[0038] Furthermore, the yellow light illumination system includes a bulb 38 and a generator switch 39 . The bulb 38 is disposed at the lower end of the V-shaped groove 28 , and the generator switch 39 is disposed at the lower end of the V-shaped groove 28 .

[0039] In this embodiment, after the mouse tail is fixed on the tail placement board 23, the light bulb 38 is turned on by the generator switch 39, thereby illuminating the position where intravenous injection is required, so that the tail vein is clearly imaged, making it convenient to observe the clear vein for injection and adjust the injection position as needed. The display of the tail vein is adjusted as needed by the switch.

[0040] The beneficial effects of the present invention are as follows: the open cylinder 2 of the device is designed to ensure that the mouse can be easily placed in, without the need to use brute force to drag it, thus avoiding accidental damage to the experimental mouse; the double nut locking method allows for quick fixation and loosening, making it convenient for the operator to operate; it is easy to adjust, fix, and loosen according to the size of the mouse, making the mouse more firmly fixed and easier to loosen and remove; the designed mouse tail clamping device is conducive to the clamping or loosening of the mouse tail, preventing the mouse tail from shaking, facilitating subsequent experiments, and not causing discomfort to the mouse. It is conducive to axially adjusting the clamping position of the mouse tail for convenient intravenous injection. It is convenient to pull out the mouse tail for intravenous injection, etc. The lighting lamp allows for clear visualization of the tail vein, making it easy to see the tail vein and speeding up the operation process; the fixing device is integrated and portable, and each component can be quickly assembled and disassembled, which is also conducive to cleaning dirt; the inclination angle of the operating table is adjustable, facilitating convenient injection operations by different operators at different heights.

[0041] In summary, the present invention has a reasonable structural design, convenient adjustment, reliable fixation, good comfort, easy use, and can complete the tail vein injection operation in a relatively short time; it causes little damage to the mouse during fixation, and the tightness of the fixation can be adjusted arbitrarily according to the size of the mouse; the fixing device is an integrated design and portable, and is a good helper for scientific researchers; the open structure is easy to clean; the inclination angle of the operating table can be adjusted to facilitate the operator to perform convenient injection operations, and it has good practicality and adaptability.

[0042] See also Figures 1 to 7 The present invention also provides a fixation method for mouse tail vein injection, comprising the following steps: In the first step, the locking screw 17 is loosened and the movable plate 16 is raised to ensure that the supporting panel 1 is in a horizontal state; Step 2: Fix the L-shaped tail blocking plate 29 to the support panel 1 by tightening the first locking bolt 30 and the fourth locking nut 31; Step 3: Tighten the second locking bolt 35 and the fifth locking nut 36 by axially adjusting the locking mechanism to preliminarily fix the tail pressing mechanism and the yellow light lighting system on the support panel 1; Step 4: Fix the pressing plate 6 to the screw rod 3 using the two first locking nuts 5 according to the length and shape of the mouse, and preliminarily adjust the positions of the two second locking nuts 7 on the screw rod 3; Step 5: Install the mouse head into the open cylinder 2 with the tail on the left and the mouse tail on the right. The right side of the open cylinder 2 is placed on the L-shaped blocking mechanism at the tail, and the bottom is placed flat on the support panel 1. Then push the screw rod 3 and the right pressure plate 6 into the open cylinder 2, and put the left side of the screw rod 3 into the U-shaped groove of the support plate 4. Step 6: Finally, observe the condition of the mouse and adjust the two opening locking mechanisms and the four circumferential pressing mechanisms in sequence to comfortably position the mouse in the open cylinder 25, and then fix the open cylinder 25 to the support panel 1; Step 7: Determine the axial position of the tail clamping mechanism based on the tail vein injection location and axially adjust and tighten the two second locking bolts 35 and the two fifth nuts to achieve locking. Then, rotate the vertical lifting rod 22 and, under the guidance of the guide mechanism 21, drive the clamping block 20 downward without rotating, thereby comfortably clamping the mouse's tail. In the eighth step, the left side of the support panel 1 is raised by lowering the movable plate 16, thereby tilting the entire panel downward to a suitable position for intravenous injection. Finally, after soaking the mouse's tail with hot water, the light is turned on to clearly display the distribution of the mouse's tail veins, and the injection is ready. Subsequently, the light bulb 38 is turned on by the generator switch 39, and the mouse is placed in a lateral position to find the lateral tail vein. If the lateral tail vein is not clear, the dorsal tail vein is found. Under the illumination of the yellow light, the tail vein will be clearly presented. Generally, the lateral tail vein is found first. If the lateral tail vein is not clear, the dorsal tail vein is found.

[0043] After finding the puncture point, use alcohol to disinfect the injection site, lock the axial position of the clamping mechanism, and press down the clamp to further expand the blood vessels. Then, pinch the tip of the mouse's tail with one hand to prevent it from swinging due to the mouse's struggle during the injection; Hold the syringe with your other hand, with the eye of the needle facing upwards. Place the entire needle against the tail and slowly insert it parallel to the tail vein. Generally, the tail vein can be punctured when the needle is about 1-1.5 mm deep. After successful puncture, push injection can be performed. If the injection volume is less than 100 μL, it can be injected quickly to prevent the mouse from struggling and getting out of the needle. If the injection volume is large, the flow rate must be controlled and not too fast. After the injection is completed, wait a few seconds before withdrawing the needle. Withdraw the needle quickly and then immediately use hemostatic clips or other methods to stop bleeding. After the injection is completed, turn off the lights in sequence, loosen the tail clamping mechanism, remove the two opening locking mechanisms and the axial clamping screw 3, take out the mouse, and complete the entire mouse intravenous injection.

[0044] The above disclosure is merely one or more preferred embodiments of the present application and is not intended to limit the scope of the present application. A person skilled in the art will understand that all or part of the processes of the above embodiments and equivalent changes made in accordance with the claims of the present application are still within the scope of the present application.

Claims

1. A fixation device for mouse tail vein injection, characterized in that: It includes a support panel, an axial pressing mechanism, an open cylinder, two opening locking mechanisms, four circumferential pressing mechanisms and an overall tilt-adjustable support mechanism; The axial clamping mechanism includes a screw rod, a support plate, two first locking nuts, a pressure plate, two second locking nuts and a lower pressure arc plate. The open cylinder is arranged on the support panel. The support plate is a U-shaped opening. The support plate is arranged on the support panel and is located on the left side of the open cylinder. The screw rod is placed on the support plate, and the right end of the screw rod is located inside the open cylinder. The pressure plate is sleeved on the outside of the screw rod. The two second locking nuts are respectively adapted to the screw rod and are respectively located on both sides of the pressure plate. The lower pressure arc plate is fixedly connected to the right end of the pressure plate. The two first locking nuts are respectively adapted to the screw rod and are respectively located on both sides of the support plate.

2. The fixation device for mouse tail vein injection according to claim 1, wherein: Each of the opening locking mechanisms includes two U-shaped opening plates, two third locking nuts and a first screw, and each of the circumferential pressing mechanisms includes a second screw and a first fixing plate. The two U-shaped opening plates are symmetrically arranged at the upper ends of the open cylinder, and the first screw is placed on the two U-shaped opening plates. The two third locking nuts are respectively adapted to the first screws, and the two third locking nuts are respectively abutted against the sides of the U-shaped opening plate.

3. The fixation device for mouse tail vein injection as claimed in claim 2, characterized in that: The overall inclination-adjustable support mechanism includes a second fixed plate, a waist-shaped fixed plate, a movable plate and a locking screw. The waist-shaped fixed plate has a waist-shaped hole. The second fixed plate is fixedly connected to the lower end of the support panel. The waist-shaped fixed plate is fixedly connected to the lower end of the support panel and is located on the left side of the second fixed plate. One end of the locking screw passes through the waist-shaped hole and is adapted to the movable plate.

4. The fixation device for mouse tail vein injection as claimed in claim 3, characterized in that: The fixation device for mouse tail vein injection also includes a tail pressing mechanism, a tail L-shaped blocking mechanism, an axial adjustment locking mechanism and a yellow light illumination system; The tail clamping mechanism includes a U-shaped plate, a pressure block, two guide mechanisms, a vertical lifting rod and a tail placement plate, the pressure block includes an inverted V-shaped clamping plate and a fixed block, the fixed block has sliding grooves on both sides, the middle of the fixed block has a thread, the tail placement plate has a V-shaped groove, the axial adjustment locking mechanism is arranged on the upper end of the support panel, the tail placement plate is arranged on the upper end of the axial adjustment locking mechanism, the U-shaped plate is fixedly arranged on the upper end of the tail placement plate, the two guide mechanisms are symmetrically arranged on the U-shaped plate, the pressure block is located between the two guide mechanisms, the two guide mechanisms are slidably adapted to the corresponding sliding grooves, the vertical lifting rod passes through the U-shaped plate and is adapted to the thread.

5. The fixation device for mouse tail vein injection according to claim 4, characterized in that: The tail L-shaped blocking mechanism includes an L-shaped tail blocking plate, a first locking bolt and a fourth locking nut. The L-shaped tail blocking plate has a U-shaped opening. The L-shaped tail blocking plate is arranged at the upper end of the support panel. The first locking bolt passes through the support panel and the L-shaped tail blocking plate. The fourth locking nut is arranged on the first locking bolt. The fourth locking nut is against the L-shaped tail blocking plate. The lower end of the inverted V-shaped clamping plate has a rubber pad.

6. The fixation device for mouse tail vein injection according to claim 5, characterized in that: The axial adjustment locking mechanism includes two L-shaped plates, two second locking bolts, and two fifth locking nuts. The support panel has two strip-shaped slots. The two L-shaped plates are respectively placed on the upper ends of the support panel. The two second locking bolts pass through the corresponding strip-shaped slots respectively. The two fifth locking nuts are respectively adapted to the corresponding second locking bolts, and the two fifth locking nuts are abutted against the corresponding L-shaped plates. The tail placement plate is fixedly arranged on the upper ends of the two L-shaped plates, and the L-shaped tail blocking plate is located between the two L-shaped plates.

7. The fixation device for mouse tail vein injection according to claim 6, wherein: The yellow light illumination system includes a bulb and a generator switch. The bulb is arranged at the lower end of the V-shaped groove, and the generator switch is arranged at the lower end of the V-shaped groove.

8. A method for fixing a mouse tail vein injection, using the fixing device for mouse tail vein injection according to claim 7, characterized in that: The steps include: In the first step, the locking screw is loosened, and the movable plate is raised to ensure that the supporting panel is in a horizontal state; Step 2: Fix the L-shaped tail blocking plate to the support panel by tightening the first locking bolt and the fourth locking nut; Step 3: Tighten the second locking bolt and the fifth locking nut by axially adjusting the locking mechanism to preliminarily fix the tail clamping mechanism and the yellow light lighting system to the support panel; Step 4: Fix the pressing plate to the screw rod using the two first locking nuts according to the length and shape of the mouse, and preliminarily adjust the positions of the two second locking nuts on the screw rod; Step 5: Install the mouse head into the open cylinder with the tail on the left and the right side on the right side. The right side of the open cylinder should be placed on the L-shaped blocking mechanism at the tail, and the bottom should be placed flat on the support panel. Then push the screw and the right pressure plate into the open cylinder, and put the left side of the screw into the U-shaped groove of the support panel. Step 6: Finally, observe the condition of the mouse and adjust the two opening locking mechanisms and the four circumferential pressing mechanisms in sequence to comfortably position the mouse in the open cylinder 5, and then fix the open cylinder 5 to the support panel; Step 7: Determine the axial position of the tail clamping mechanism based on the tail vein injection location and axially adjust and tighten the two second locking bolts and the two fifth nuts to achieve locking. Then, rotate the vertical lifting rod and, under the guidance of the guide mechanism, drive the clamping block downward without rotating, thereby comfortably clamping the mouse's tail. In the eighth step, the left side of the support panel is raised by lowering the movable plate, thereby tilting the entire device downward to a suitable position for intravenous injection. Finally, after soaking the mouse's tail with hot water, the light is turned on to clearly display the distribution of the mouse's tail veins, and the injection is ready. Then, the light bulb is turned on by the generator switch, and the mouse is placed in a lateral position. Under the illumination of the yellow light, the tail vein will be clearly visible; Generally, the lateral tail vein is found first. If the lateral tail vein is not clear, the dorsal tail vein is found. After finding the puncture point, use alcohol to disinfect the injection site, lock the axial position of the clamping mechanism, and press down the clamp to further expand the blood vessels. Then, pinch the tip of the mouse's tail with one hand to prevent it from swinging due to the mouse's struggle during the injection; Hold the syringe with your other hand, with the eye of the needle facing upwards. Place the entire needle against the tail and slowly insert it parallel to the tail vein. Generally, the tail vein can be punctured when the needle is about 1-1.5 mm deep. After successful puncture, push injection can be performed. If the injection volume is less than 100 μL, it can be injected quickly to prevent the mouse from struggling and getting out of the needle. If the injection volume is large, the flow rate must be controlled and not too fast. After the injection is completed, wait a few seconds before withdrawing the needle. Withdraw the needle quickly and then immediately use hemostatic clips or other methods to stop bleeding. After the injection is completed, turn off the lights, loosen the tail clamping mechanism, remove the two opening locking mechanisms and the axial clamping screw, take out the mouse, and complete the entire intravenous injection of the mouse.

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