Method for treating blackening and odor returning water body by sulfur autotrophic denitrification and anaerobic ammonia oxidation

Abstract

This invention discloses a method for treating blackened and odorous water bodies using a combination of sulfur autotrophic denitrification and anaerobic ammonium oxidation. The method includes: collecting overlying water and sediment samples from the blackened and odorous water body; sealing and allowing the overlying water and sediment samples to stand to obtain a treatment sample; determining the content of volatile organic sulfur compounds in the sediment sample and calculating the theoretical dosage of calcium nitrate; adding 1.0 times the theoretical dosage of calcium nitrate to the sediment of the treatment sample, and adding SAD sludge and Anammox sludge to the surface layer of the sediment; continuously operating until any day between days 20 and 30, then adding 0.3 to 0.8 times the theoretical dosage of calcium nitrate; continuing operation until days 45 to 55 to complete the water body treatment. This invention not only effectively solves the problem of water quality deterioration caused by seasonal changes but also synergistically removes NH4 from the sediment. + It contains AVS and organic matter, thereby reducing the emission of greenhouse gases such as N2O during sediment remediation.

Description

Technical Field

[0001] This invention belongs to the field of pollution remediation of black and odorous water bodies in polluted rivers / lakes, and particularly relates to a method for treating black and odorous water bodies by sulfur autotrophic denitrification combined with anaerobic ammonia oxidation. Background Technology

[0002] The nitrogen and sulfur cycle is an important component of the geochemical element cycle, and the formation of black and odorous water bodies is closely related to the obstruction of the nitrogen and sulfur cycle process in the sediment. The discharge of domestic sewage and industrial wastewater leads to the accumulation of pollutants such as organic matter, volatile organic sulfur compounds (AVS) and ammonia nitrogen in the sediment, which not only destroys the self-purification capacity of the water body, but also inhibits the nitrogen and sulfur cycle. In addition, pollutants in the sediment will be released into the overlying water layer, further deteriorating the water quality. For some treated rivers and lakes, due to the input of external pollutants from rainfall or the incompleteness of the original treatment measures, different degrees of blackening and odor recurrence will occur. Compared with black and odorous water bodies, blackening and odor recurrence water bodies generally have the following characteristics: (1) the degree of pollution is relatively light; (2) the pollution type is mainly short-term external source; (3) the water quality is prone to repeated changes. Therefore, traditional black and odorous water body treatment strategies, such as sediment dredging, are not entirely suitable for the treatment of blackening and odor recurrence water bodies, because these methods often have the problem of excessive cost, which is difficult to meet the needs of such water bodies for rapid restoration, low budget and convenient treatment. To effectively address these issues, strengthening the nitrogen and sulfur cycle in the sediment has become crucial for treating black and odorous water bodies.

[0003] In-situ remediation and ex-situ remediation are two commonly used techniques for sediment remediation. In-situ remediation, in particular, has gained significant attention due to its lower cost and ability to promote ecosystem restoration. Currently, aeration, the addition of microbial agents, chemical agents, and their combinations are frequently used for the remediation of black and odorous sediments. The addition of calcium nitrate (CN) is considered one of the most effective methods, primarily by oxidizing reducing agents and increasing the oxidation-reduction potential (ORP), thereby improving the microbial growth environment and achieving sediment remediation. Microorganisms in river and lake sediments play a crucial role in the Earth's nitrogen and sulfur cycles. The addition of CN can not only effectively remove organic matter and volatile organic sulfur compounds (AVS) from sediments but also promote the growth of sulfur-oxidizing bacteria (SOBs). However, large amounts of NO3... - The addition of [a substance] promotes the dissimilatory reduction of nitrate to ammonium (DNRA) and denitrification (DNF), leading to an increase in NH4+ in the overlying water. + and NO x - Concentration increases. Furthermore, Ca... 2+ Increased CN concentration can also lead to increased salinity in the sediment. These problems can be mitigated to some extent by preparing slow-release CN materials or improving the dosing device. However, due to NO3... - This promotes the occurrence of DNRA and results in extremely low levels of ORP and NH4 in the sediment environment.+ It remains difficult to remove. Furthermore, river and lake basins are significant sources of greenhouse gas emissions; studies show that river CO2 emissions account for 25% of the total CO2 released from fossil fuel combustion, offsetting approximately 68% of terrestrial carbon sinks. Adding CN will promote denitrification, leading to the release of greenhouse gases such as N2O, which contradicts current carbon reduction policies.

[0004] Chinese invention patent CN113943051A, entitled "A Method for Enhancing the Activity of Nitrogen and Sulfur Co-circulation in Riverbed Sediments," describes a technique for remediating riverbed sediments by cultivating anaerobic ammonia oxidation (Anammox) coupled with sulfur autotrophic denitrification (SAD) bacteria. However, due to the relatively mild pollution levels in the re-blackening and odor-causing water bodies, and the presence of NO3... - The content is too low; simply adding Anammox to couple with SAD bacteria is insufficient to reduce NH4 in the sediment. + Simultaneous removal of organic matter and volatile organic sulfur compounds (AVS) is required. Furthermore, coupling SAD with Anammox is relatively difficult; changes in environmental conditions can significantly reduce microbial activity, making it difficult to maintain the mass ratio of anaerobic ammonia oxidizing bacteria (AAOB) to sulfur oxidizing bacteria (SOB), thus leading to reduced pollutant removal efficiency. Summary of the Invention

[0005] To address the shortcomings of existing technologies, the present invention aims to provide a method for treating blackened and odorous water bodies through a combination of sulfur autotrophic denitrification (SAD) and anammox. This invention addresses the deficiencies of traditional technologies in dealing with the problem of water bodies turning black and odorous due to seasonal changes by proposing a solution involving the time-phased and zone-based addition of calcium nitrate (CN) combined with sulfur autotrophic denitrification (SAD) and anammox sludge treatment. This method not only effectively solves the problem of water quality deterioration caused by seasonal changes but also synergistically removes NH4 from the bottom sediment. + It contains AVS and organic matter, thereby reducing the emission of greenhouse gases such as N2O during sediment remediation.

[0006] Furthermore, by implementing the technical solution provided by this invention, the activity and abundance of microorganisms involved in denitrification (DNF), anaerobic ammonia oxidation (Anammox), dissimilatory reduction of nitrate to ammonium (DNRA), sulfur oxidation, and sulfur reduction processes in the sediment can be significantly enhanced, thereby promoting the efficient nitrogen and sulfur cycles within the sediment. This not only helps restore the water body's own purification capacity but also continuously improves the water quality that has already experienced blackening and odor recurrence, and effectively prevents similar problems from recurring in treated water bodies. In summary, this invention provides an environmentally friendly and cost-effective novel water treatment strategy, which is of great significance for ensuring water environmental quality.

[0007] The objective of this invention is achieved through the following technical solution:

[0008] A method for treating blackened and odorous water bodies through a combination of sulfur autotrophic denitrification and anaerobic ammonia oxidation includes the following steps:

[0009] Overlying water and bottom sediment samples were collected from the blackened and smelly water bodies. The treatment samples were obtained by sealing and allowing the overlying water and bottom sediment samples to stand.

[0010] The content of volatile organic sulfur compounds in the sediment sample was determined, and the theoretical dosage of calcium nitrate was calculated.

[0011] Add 1.0 times the theoretical dosage of calcium nitrate to the bottom sediment of the treatment sample, and add SAD sludge and Anammox sludge to the surface layer of the bottom sediment of the treatment sample. Continue to run the test until any day between the 20th and 30th day, then add 0.3 to 0.8 times the theoretical dosage of calcium nitrate. Continue to run the test until the 45th to 55th day to complete the treatment of the blackened and smelly water body.

[0012] Preferably, the overlying water sample is taken from 0.5 to 1 m below the water surface.

[0013] Preferably, the sediment sample is taken from 10-20 cm below the overlying water.

[0014] Preferably, large particles are removed from the sediment sample before the content of volatile organic sulfur compounds is determined.

[0015] Preferably, the sediment sample is homogenized before being sealed and allowed to stand. More preferably, the sealing and standing time is 1 to 5 days.

[0016] Preferably, the homogenization process involves stirring the sediment sample at a rate of 200 rpm for 30 minutes.

[0017] Preferably, in the treatment sample, the volume ratio of the bottom sediment sample to the overlying water sample is 1:3.

[0018] Preferably, calcium nitrate is added in the form of an aqueous solution. It should be ensured that the calcium nitrate is completely dissolved in the water; there are no particular restrictions on the amount of water added.

[0019] Preferably, the volume ratio of SAD sludge to Anammox sludge is 1~3:1;

[0020] Preferably, the total volume ratio of SAD sludge and Anammox sludge to the volume of the sediment sample is 1:28~113.

[0021] Preferably, the conditions for continuous operation are: operating temperature controlled at 30±5℃, and operation in the dark.

[0022] Preferably, SAD sludge Sulfurimonas and / or ThiobacillusThe relative abundance was higher than 5%, and the MLSS was higher than 3000 mg / L. More preferably, the SAD sludge... Sulfurimonas and Thiobacillus The relative abundances were 6.3–8.3% and 3.3–5.3%, respectively, and the MLSS was 3521–3655 mg / L.

[0023] Preferably, Aanmmox sludge contains Candidatus_Brocadia and / or Candidatus_Jettenia The relative abundance was higher than 5%, and the MLSS was higher than 3000 mg / L. More preferably, the abundance in Aanmmox sludge... Candidatus_Brocadia and Candidatus_Jettenia The relative abundances were 10.1–12.1% and 3.6–4.6%, respectively, and the MLSS was 3651–4012 mg / L.

[0024] Compared with the prior art, the beneficial effects of the present invention include:

[0025] (1) By adding CN solution at different times, this invention can effectively control and reduce the excessive levels of TN and NO in the overlying water caused by the large release of CN in a short period of time. x - The problem of abnormal emissions. Furthermore, this method can also alleviate the problem caused by NO3. - NH4+ in overlying water that promotes the DNRA process + An abnormally high concentration.

[0026] (2) This invention employs a time-segmented and zoned approach to apply CN, SAD, and Anammox sludge for the remediation of black and odorous water bodies, significantly improving treatment efficiency. During this process, NO3... - It not only promotes autotrophic and heterotrophic denitrification, but also promotes the conversion of AVS to higher valence states of sulfur, such as S. 0 SO4 2- Transformation, and generation of NO2 - These NO2 - It can then react with ammonia nitrogen via the Anammox pathway to produce N2. Simultaneously, the DNRA process can also remove some NO3. - Converted to NH4 + This promotes the recycling of nitrogen and sulfur elements throughout the system.

[0027] (3) In this invention, SAD and Anammox technologies do not require complex coupling operations beforehand. They can achieve good sediment remediation effects simply by mixing them in a certain proportion, which greatly reduces the cost and technical difficulty in practical applications and makes them easier to promote and implement.

[0028] (4) In this invention, NO2 generated during the DNF process -The nitrogen will be consumed by microorganisms in Anammox sludge, which not only reduces the emissions of greenhouse gases such as N2O that may be generated during the continuous reduction to nitrogen, but also has important significance for achieving the goals of "carbon peaking" and "carbon neutrality".

[0029] (5) This invention combines CN, SAD sludge, and Anammox sludge to remediate water bodies that have exhibited signs of blackening and foul odor. By enhancing multiple biochemical reaction processes within the sediment, such as DNF, Anammox, DNRA, sulfur oxidation, and sulfur reduction, a highly efficient nitrogen and sulfur cycle system is constructed. This comprehensive treatment method not only effectively improves water quality but also maintains the healthy and stable development of the ecosystem in the long term. Attached Figure Description

[0030] Figure 1 To address the remediation of blackened and odorous water bodies using the sulfur autotrophic denitrification synergistic anaerobic ammonia oxidation method described in Example 1, AVS and NH4 in the bottom sediment were measured before and after operation. + A comparison chart of changes in TN.

[0031] Figure 2 This is a comparison chart showing the changes in DNF, Anammox, DNRA, and sulfur oxidation rate in the bottom sediment before and after the implementation of the sulfur autotrophic denitrification synergistic anaerobic ammonia oxidation method for treating blackened and odorous water bodies as described in Example 1.

[0032] Figure 3 To address the re-blackening and odorization of water bodies using the sulfur autotrophic denitrification synergistic anaerobic ammonia oxidation method described in Example 2, AVS and NH4 in the bottom sediment were measured before and after operation. + A comparison chart of changes in TN.

[0033] Figure 4 To implement the method for treating blackened and odorous water bodies described in Comparative Example 3, AVS and NH4 in the bottom sediment were measured before and after operation. + A comparison chart of changes in TN.

[0034] Figure 5 This is a comparison chart showing the changes in DNF, Anammox, DNRA, and sulfur oxidation rate in the bottom sediment before and after the implementation of the sulfur autotrophic denitrification synergistic anaerobic ammonia oxidation method for treating blackened and odorous water bodies as described in Example 2.

[0035] Figure 6 The graph shows a comparison of the changes in DNF, Anammox, DNRA, and sulfur oxidation rate in the bottom sediment before and after operation, using the method described in Comparative Example 3 for treating blackened and odorous water bodies.

[0036] Figure 7To address the re-blackening and odorization of water bodies using the sulfur autotrophic denitrification synergistic anaerobic ammonia oxidation method described in Example 3, AVS and NH4 in the bottom sediment were measured before and after operation. + A comparison chart of changes in TN.

[0037] Figure 8 This is a comparison chart showing the changes in DNF, Anammox, DNRA, and sulfur oxidation rate in the bottom sediment before and after the implementation of the sulfur autotrophic denitrification synergistic anaerobic ammonia oxidation method for treating blackened and odorous water bodies as described in Example 3. Detailed Implementation

[0038] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0039] The following is a description of the sources of various sludges involved in the examples and comparative examples:

[0040] The samples of the blackened and foul-smelling water and sediment were collected from a lake in Wuhan City. This lake had previously undergone comprehensive remediation measures including dredging, aeration, and vegetation planting. However, due to the failure of the remediation techniques to restore the lake's nitrogen and sulfur cycle capacity, factors such as the discharge of external sewage and seasonal changes led to the lake reverting to its blackened and foul-smelling state. Sediment samples were taken from 10–20 cm below the overlying water surface, and overlying water samples were taken from 0.5–1 m below the water surface. Overlying water samples were filtered through a 0.45 μm pore size membrane, while sediment samples were tested for pollution indicators after removing large particles (particle size > 2 mm). Some sediment samples were stored at -20°C, while the remaining samples were sealed in plastic containers.

[0041] SAD sludge, Anammox sludge, and S / A sludge originated from the stably operating SAD reactor, Anammox reactor, and sulfur autotrophic reactive sludge nitrification coupled with anaerobic ammonium oxidation (S / A) reactor, respectively. The SAD, Anammox, and S / A reactors are all stably operating reactors in Laboratory South 506, School of Resources and Environmental Engineering, Wuhan University of Technology.

[0042] The formula for calculating the theoretical dosage of calcium nitrate is: ,

[0043] In the formula: C CN — The theoretical dosage of calcium nitrate required is calculated in nitrogen (mg / kg);

[0044] C AVS — AVS content in sediment (mg / kg);

[0045] M N — Molar mass of N (g / mol);

[0046] M N1 — NO3 - It is reduced to the N2 oxidation state change (5 valence);

[0047] M S1 — S 2- Oxidized to SO4 2- Changes in valence (octet);

[0048] M S — Molar mass of S (g / mol).

[0049] Example 1

[0050] The SAD sludge Sulfurimonas and Thiobacillus The relative abundances were 8.3% and 3.3%, respectively, and the MLSS was 3655 mg / L.

[0051] In the Aanmmox sludge Candidatus_Brocadia and Candidatus_Jettenia The relative abundances were 12.1% and 3.6%, respectively, and the MLSS was 4012 mg / L.

[0052] A method for treating blackened and odorous water bodies using a combination of sulfur autotrophic denitrification and anaerobic ammonia oxidation comprises the following steps:

[0053] (1) Collect samples of the overlying water and bottom sediment of the blackened and smelly water body. After removing large particles, measure the pollution indicators of the bottom sediment samples. The TN and NH4 in the bottom sediment were measured. + The AVS content is 1034.9 mg / kg, 498.9 mg / kg and 1179.7 mg / kg respectively. Calculate the theoretical dosage of calcium nitrate based on the AVS content.

[0054] (2) An experiment was conducted for 45 days in a cylindrical simulation system with a diameter of 12 cm and a height of 90 cm. During this period, the system was kept in the dark and the temperature was controlled at 30 ± 5 °C. First, the sediment sample was stirred at 200 rpm for 30 min to achieve homogenization. Then, the treated sediment was added to the system to form a 20 cm thick sediment layer. After removing DO with N2, the overlying water was siphoned into the system until the liquid level reached 80 cm. At this point, the volume ratio of sediment to overlying water was 1:3. After the system was sealed and allowed to stand for 3 days, the treated sample was obtained, and the experiment officially began.

[0055] (3) On day 0 and day 20, 0.355 g and 0.178 g of calcium nitrate were dissolved in 10 mL of water, respectively, and the solution was injected into the middle of the sediment. Here, the amount of calcium nitrate added on day 0 was 1.0 times the theoretical amount of calcium nitrate calculated in step (1), and the amount of calcium nitrate added on day 20 was 0.5 times the theoretical amount of calcium nitrate calculated in step (1). The total amount of calcium nitrate added was 1.5 times the theoretical amount of calcium nitrate. In addition, on day 0, a mixture of SAD sludge and Anammox sludge with a volume ratio of 3:1 (60 mL in total) was added to the surface of the sediment. The system ran continuously for 45 days to complete the treatment of the blackened and smelly water.

[0056] Comparative Example 1

[0057] Comparative Example 1 was a blank control. The only difference between Comparative Example 1 and Example 1 was that Comparative Example 1 did not involve the addition of calcium carbonate, SAD sludge, or Anammox sludge. After the experiment officially started, no operation was performed, and it was run continuously for 45 days.

[0058] After the systems of Example 1 and Comparative Example 1 ran continuously for 45 days, tests and calculations showed that the NH4 content in the sediment of Example 1 was... + Compared to before operation, AVS was reduced by 75% and 82%; the DNF, Anammox, DNRA, and sulfur oxidation rates in Example 1 were 1.57 times, 3.86 times, 2.42 times, and 1.41 times that of Comparative Example 1, respectively. In the sediment of Example 1, the dominant bacterial community... Acinetobacter Transform into Thiobacillus , Thiobacillus and Candidatus_Brocadia The relative abundance of these increased to 3.77% and 0.92%, which were 2.68 times and 8.98 times that of Comparative Example 1, respectively.

[0059] Example 2

[0060] The SAD sludge Sulfurimonas and Thiobacillus The relative abundances were 8.3% and 3.3%, respectively, and the MLSS was 3655 mg / L.

[0061] In the Aanmmox sludge Candidatus_Brocadia and Candidatus_Jettenia The relative abundances were 12.1% and 3.6%, respectively, and the MLSS was 4012 mg / L.

[0062] A method for treating blackened and odorous water bodies using a combination of sulfur autotrophic denitrification and anaerobic ammonia oxidation comprises the following steps:

[0063] (1) Collect samples of the overlying water and bottom sediment of the blackened and smelly water body. After removing large particles, measure the pollution indicators of the bottom sediment samples. The TN and NH4 in the bottom sediment were measured.+ The AVS content was 386.9 mg / kg, 315.4 mg / kg and 1367.8 mg / kg, respectively. The theoretical dosage of calcium nitrate was calculated based on the AVS content.

[0064] (2) An experiment was conducted for 55 days in a cylindrical simulation system with a diameter of 12 cm and a height of 90 cm. During this period, the system was kept in the dark and the temperature was controlled at 30 ± 5 °C. First, the sediment sample was stirred at 200 rpm for 30 min to achieve homogenization. Then, the treated sediment was added to the system to form a 20 cm thick sediment layer. After removing DO with N2, the overlying water was siphoned into the system until the liquid level reached 80 cm. At this point, the volume ratio of sediment to overlying water was 1:3. After the system was sealed and allowed to stand for 3 days, the treated sample was obtained, and the experiment officially began.

[0065] (3) On day 0 and day 30, 0.355 g and 0.106 g of calcium nitrate were dissolved in 10 mL of water, respectively, and the solution was injected into the middle of the sediment. Here, the amount of calcium nitrate added on day 0 was 1.0 times the theoretical amount of calcium nitrate calculated in step (1), and the amount of calcium nitrate added on day 30 was 0.3 times the theoretical amount of calcium nitrate calculated in step (1). The total amount of calcium nitrate added was 1.3 times the theoretical amount of calcium nitrate. In addition, on day 0, a mixture of SAD sludge and Anammox sludge with a volume ratio of 2:1 (30 mL in total) was added to the surface of the sediment. The system ran continuously for 55 days to complete the treatment of the blackened and smelly water.

[0066] Comparative Example 2

[0067] Comparative Example 2 was a blank control. The only difference between Comparative Example 2 and Example 2 was that Comparative Example 2 did not involve the addition of calcium nitrate, SAD sludge, or Anammox sludge. After the experiment officially started, no operation was performed, and it was run continuously for 55 days.

[0068] Comparative Example 3

[0069] Comparative Example 3 served as the experimental control. The only difference from Example 2 was that Comparative Example 3 did not add SAD sludge or Anammox sludge; instead, it added 30 mL of S / A sludge. All other procedures were the same as in Example 2. Specifically, the S / A sludge contained… Candidatus_Brocadia and of Candidatus_Jettenia The relative abundances were 3.4% and 0.4%. Sulfurimonas , Thiobacillus and of Denitratisoma The relative abundances were 10.2%, 9.1%, and 3.4%; the MLSS was 3179 mg / L.

[0070] After continuous operation of the systems in Example 2 and Comparative Examples 2-3 for 55 days, tests and calculations showed that the NH4 content in the sediment of Example 2 was... + Compared to before remediation, AVS reduced NH4 by 80% and 83%; compared to the previous level, NH4 in the sediment of Comparative Example 3 was reduced. + Compared to before the repair, AVS and DNF levels were reduced by 82% and 76%, respectively. The DNF, Anammox, DNRA, and sulfur oxidation rates in Example 2 were 1.57 times, 3.86 times, 2.42 times, and 1.41 times that of Comparative Example 2, respectively. The DNF, Anammox, DNRA, and sulfur oxidation rates in Comparative Example 3 were 1.48 times, 3.85 times, 2.41 times, and 1.47 times that of Comparative Example 2, respectively.

[0071] The dominant bacterial communities in the sediment of Examples 2 and 3 were obtained from... Transform into Acinetobacter Example 2: Bottom mud Thiobacillus and Thiobacillus The relative abundances increased to 3.77% and 0.92%, respectively; in the bottom sediment of Comparative Example 3... Candidatus_Brocadia and Thiobacillus The relative abundances of these two elements increased to 3.25% and 0.95%, respectively.

[0072] Example 3

[0073] The SAD sludge Candidatus_Brocadia and Sulfurimonas The relative abundances were 6.3% and 5.3%, respectively, and the MLSS was 3521 mg / L.

[0074] In the Aanmmox sludge Thiobacillus and Candidatus_Brocadia The relative abundances were 10.1% and 4.6%, respectively, and the MLSS was 3651 mg / L.

[0075] A method for treating blackened and odorous water bodies using a combination of sulfur autotrophic denitrification and anaerobic ammonia oxidation comprises the following steps:

[0076] (1) Collect samples of the overlying water and bottom sediment of the blackened and smelly water body. After removing large particles, measure the pollution indicators of the bottom sediment samples. The TN and NH4 in the bottom sediment were measured. + The AVS contents are 421.1 mg / kg, 326.9 mg / kg and 1428.9 mg / kg, respectively. Calculate the theoretical dosage of calcium nitrate based on the AVS content.

[0077] (2) An experiment was conducted for 50 days in a cylindrical simulation system with a diameter of 12 cm and a height of 90 cm. During this period, the system was kept in the dark and the temperature was controlled at 30 ± 5 °C. First, the sediment sample was stirred at 200 rpm for 30 min to achieve homogenization. Then, the treated sediment was added to the system to form a 20 cm thick sediment layer. After removing DO with N2, the overlying water was siphoned into the system until the liquid level reached 80 cm. At this point, the volume ratio of sediment to overlying water was 1:3. After the system was sealed and allowed to stand for 3 days, the treated sample was obtained, and the experiment officially began.

[0078] (3) On day 0 and day 30, 0.371 g and 0.297 g of calcium nitrate were dissolved in 10 mL of water, respectively, and the solution was injected into the middle of the sediment. Here, the amount of calcium nitrate added on day 0 was 1.0 times the theoretical amount of calcium nitrate calculated in step (1), and the amount of calcium nitrate added on day 30 was 0.8 times the theoretical amount of calcium nitrate calculated in step (1). The total amount of calcium nitrate added was 1.8 times the theoretical amount of calcium nitrate. In addition, on day 0, a mixture of SAD sludge and Anammox sludge with a volume ratio of 1:1 (40 mL in total) was added to the surface of the sediment. The system was run continuously for 50 days to complete the treatment of the blackened and smelly water.

[0079] Comparative Example 4

[0080] Comparative Example 4 was a blank control. The only difference between Comparative Example 4 and Example 3 was that Comparative Example 4 did not involve the addition of calcium nitrate, SAD sludge, or Anammox sludge. After the experiment officially started, no operation was performed, and the experiment was run continuously for 50 days.

[0081] After the systems of Example 3 and Comparative Example 4 were run continuously for 50 days, tests and calculations showed that the NH4 content in the sediment of Example 1 was... + Compared to before operation, AVS was reduced by 75% and 81%; the DNF, Anammox, DNRA, and sulfur oxidation rates in Example 3 were 1.65 times, 3.21 times, 1.98 times, and 1.56 times that of Comparative Example 4, respectively. In the sediment of Example 4, the dominant bacterial community... Candidatus_Jettenia Transform into Acinetobacter , Thiobacillus and Thiobacillus The relative abundance of these increased to 2.15% and 0.87%.

[0082] Candidatus_Brocadia To address the resurgence of black and odorous water bodies using the sulfur autotrophic denitrification synergistic anaerobic ammonia oxidation method described in Example 1, AVS and NH4 in the bottom sediment were analyzed before and after operation. + A comparison chart of changes in TN, from Figure 1 We can see that after 45 days of modification, the levels of AVS and NH4 in the sediment have decreased.+ Both TN and TN were significantly reduced.

[0083] Figure 1 To illustrate the method for treating blackened and odorous water bodies using sulfur autotrophic denitrification combined with anaerobic ammonia oxidation as described in Example 1, a comparison chart of changes in DNF, Anammox, DNRA, and sulfur oxidation rate in the sediment before and after operation is presented. Figure 2 We can see that, after the remediation described in this invention, the nitrogen and sulfur metabolism potential of the sediment has been improved to a certain extent.

[0084] Figure 2 To address the resurgence of black and odorous water bodies using the sulfur autotrophic denitrification synergistic anaerobic ammonia oxidation method described in Example 2, AVS and NH4 in the bottom sediment were analyzed before and after operation. + A comparison chart of changes in TN. Figure 3 To implement the method for treating blackened and odorous water bodies described in Comparative Example 3, AVS and NH4 in the bottom sediment were measured before and after operation. + A comparison chart of changes in TN. By comparing... Figure 4 It can be seen that the sediment treated using the method described in Example 2 contains AVS and NH4. + The content of TN was significantly reduced, and the pollutant reduction effect was slightly better than that of the method in Comparative Example 3.

[0085] Figures 3 - 4 This is a comparison chart showing the changes in DNF, Anammox, DNRA, and sulfur oxidation rate in the bottom sediment before and after the implementation of the method for treating blackened and odorous water bodies using sulfur autotrophic denitrification synergistic with anaerobic ammonia oxidation as described in Example 2. Figure 5 This chart compares the changes in DNF, Anammox, DNRA, and sulfur oxidation rate in the bottom sediment before and after operation, using the method described in Comparative Example 3 for treating blackened and odorous water bodies. Figure 6 We can see that after the treatment methods described in Example 2 and Comparative Example 3, the nitrogen and sulfur metabolism potential of the sediment was improved to a certain extent. Further analysis shows that the improvement brought by Example 2 is slightly greater than that of Comparative Example 3, and it is simpler to operate and has a lower cost.

[0086] Figures 5 - 6 To address the re-blackening and odorization of water bodies using the sulfur autotrophic denitrification synergistic anaerobic ammonia oxidation method described in Example 3, AVS and NH4 in the bottom sediment were analyzed before and after operation. + A comparison chart of changes in TN. Figure 7 This chart compares the changes in DNF, Anammox, DNRA, and sulfur oxidation rate in the sediment before and after operation of the method for treating blackened and odorous water bodies using the sulfur autotrophic denitrification synergistic anaerobic ammonia oxidation described in Example 3. Figure 8 Figures 7 - 8 It can be seen that, after the remediation using the treatment method described in Example 3, the AVS and NH4 in the bottom sediment were effectively reduced.+ Both nitrogen and total nitrogen (TN) levels decreased significantly, while the nitrogen and sulfur metabolism potential of the sediment was also improved to some extent.

[0087] The specific embodiments of the present invention described above do not constitute a limitation on the scope of protection of the present invention. Any other corresponding changes and modifications made in accordance with the technical concept of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. A method for treating blackened and odorous water bodies through a combination of sulfur autotrophic denitrification and anaerobic ammonia oxidation, characterized in that, Includes the following steps: Overlying water and bottom sediment samples were collected from the blackened and smelly water bodies. The treatment samples were obtained by sealing and allowing the overlying water and bottom sediment samples to stand. The content of volatile organic sulfur compounds in the sediment sample was determined, and the theoretical dosage of calcium nitrate was calculated. Add 1.0 times the theoretical dosage of calcium nitrate to the bottom sediment of the treatment sample, and add SAD sludge and Anammox sludge to the surface layer of the bottom sediment of the treatment sample. Continue to run the test until any day between the 20th and 30th day, then add 0.3 to 0.8 times the theoretical dosage of calcium nitrate. Continue to run the test until the 45th to 55th day to complete the treatment of the blackened and smelly water body.

2. The method for treating blackened and odorous water bodies by sulfur autotrophic denitrification synergistic with anaerobic ammonia oxidation according to claim 1, characterized in that, The overlying water sample was taken from a depth of 0.5 to 1 m below the water surface; The sediment samples were taken from 10-20 cm below the overlying water.

3. The method for treating blackened and odorous water bodies by sulfur autotrophic denitrification synergistic with anaerobic ammonia oxidation according to claim 1, characterized in that, Large particles were first removed from the sediment samples before the content of volatile organic sulfur compounds was determined. The sediment sample was homogenized and then sealed and left to stand. The calcium nitrate was added in the form of an aqueous solution.

4. The method for treating blackened and odorous water bodies by sulfur autotrophic denitrification synergistic with anaerobic ammonia oxidation according to claim 3, characterized in that, The homogenization process involves stirring the sediment sample at a rate of 200 rpm for 30 minutes.

5. The method for treating blackened and odorous water bodies by sulfur autotrophic denitrification synergistic with anaerobic ammonia oxidation according to claim 1, characterized in that, In the treatment test samples, the volume ratio of the bottom sediment sample to the overlying water sample was 1:

3.

6. The method for treating blackened and odorous water bodies by sulfur autotrophic denitrification synergistic with anaerobic ammonia oxidation according to claim 1, characterized in that, The volume ratio of SAD sludge to Anammox sludge is 1~3:1; The total volume ratio of the SAD sludge and Anammox sludge to the sediment sample was 1:28~113. The conditions for continuous operation are: operating temperature controlled at 30±5℃, and operation in the dark.

7. The method for treating blackened and odorous water bodies by sulfur autotrophic denitrification synergistic with anaerobic ammonia oxidation according to claim 1, characterized in that, The SAD sludge Sulfurimonas and / or Thiobacillus The relative abundance was higher than 5%, and the MLSS was higher than 3000 mg / L.

8. The method for treating blackened and odorous water bodies by sulfur autotrophic denitrification synergistic with anaerobic ammonia oxidation according to claim 7, characterized in that, SAD sludge Sulfurimonas and Thiobacillus The relative abundances were 6.3–8.3% and 3.3–5.3%, respectively, and the MLSS was 3521–3655 mg / L.

9. The method for treating blackened and odorous water bodies by sulfur autotrophic denitrification synergistic with anaerobic ammonia oxidation according to claim 1, characterized in that, The Anammox sludge Candidatus_Brocadia and / or Candidatus_Jettenia The relative abundance was higher than 5%, and the MLSS was higher than 3000 mg / L.

10. The method for treating blackened and odorous water bodies by sulfur autotrophic denitrification synergistic with anaerobic ammonia oxidation according to claim 9, characterized in that, Anammox sludge Candidatus_Brocadia and Candidatus_Jettenia The relative abundances were 10.1–12.1% and 3.6–4.6%, respectively, and the MLSS was 3651–4012 mg / L.

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