Multi-channel electric shock tube

Abstract

The invention provides a multi-channel electric shock tube, and belongs to the technical field of biological medicine instruments and equipment. The problem that an existing electric shock tube is low in experiment efficiency is solved. The multi-channel electric shock tube comprises a plurality of sample containing tubes made of insulating materials, the plurality of sample containing tubes are fixed in a row, each sample containing tube is provided with a first end opening and a second end opening, and the multi-channel electric shock tube further comprises a first conductive material piece, a cover body and a second conductive material piece, the first end opening of the sample accommodating tube is blocked by the first conductive material piece, and the second end opening of the sample accommodating tube is blocked by the second conductive material piece when the sample accommodating tube is covered by the cover body. According to the multi-channel electric shock tube, a plurality of sample containing tubes are used for synchronous experiments, and the experiment efficiency is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedical instruments and equipment, and relates to a multi-channel electric shock tube. Background Art

[0002] Cell electroporation (also known as cell electrofection or cell electroporation) involves applying a high-intensity electric field to transiently increase the permeability of the cell membrane, temporarily making it porous and permeable to foreign materials, such as macromolecules. The effectiveness of cell membrane electroporation depends on various electric field parameters, such as pulse type, pulse voltage, pulse duration, number of pulses, and other experimental conditions.

[0003] The applicant has previously applied for a Chinese patent application [Authorization Announcement No.: CN104403943B] which discloses an electric shock tube and a cell electroporator having an electric shock tube. The electric shock tube includes a tube body, a first electrode, a second electrode and a plug. The tube body has a cavity for accommodating a target liquid sample. One end of the tube body is provided with a first electrode, and the other end of the tube body has an opening connected to the cavity. The working part of the first electrode is connected to the cavity, and the edge of the opening has an annular end surface. The second electrode is arranged in the plug and the outer end of the second electrode can be electrically connected to the outside through the opening of the plug. The inner end surface of the second electrode can be fitted with the annular end surface of the edge of the opening. An elastic member connected to the second electrode is provided in the plug, the outer side of the elastic member is connected to the plug, and the inner side of the elastic member is connected to the second electrode. The outer periphery of the opening has a positioning structure that can fix the plug to the end of the tube body and cause the elastic member to produce compression deformation.

[0004] The above structure allows the tube to be filled with a test sample containing cells and a substance to be injected into the cells, and then the plug is placed on the tube to close the opening. The first and second electrodes are then connected to a pulse power supply, and discharge is performed to form an electric field in the shock tube. When multiple test samples need to be tested, the above shock tube can only accommodate a single test sample, which cannot meet the requirements of conducting multiple experiments simultaneously, resulting in low experimental efficiency. Summary of the Invention

[0005] The purpose of the present invention is to address the above-mentioned problems in the existing technology and propose a multi-channel electric shock tube. The technical problem to be solved by the present invention is: how to solve the problem of low experimental efficiency of the existing electric shock tube.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] A multi-channel electroporation tube includes a plurality of sample-holding tubes made of insulating material. The plurality of sample-holding tubes are fixed in a row, each having a first opening and a second opening. The multi-channel electroporation tube further includes a first conductive member, a cover, and a second conductive member. The first conductive member seals the first openings of the sample-holding tubes, and the second conductive member seals the second openings of the sample-holding tubes when the cover is closed over the sample-holding tubes. The first and second conductive members can generally be made of metal materials such as aluminum, copper, and steel.

[0008] The multi-channel electroporation tube comprises multiple sample holding tubes, each having a first end opening sealed by a first conductive material. The sample holding tubes are filled with a liquid sample containing cells and a substance to be injected into the cells. The multi-channel electroporation tube further comprises a cover, the second end opening of which is sealed by a second conductive material. The multiple sample holding tubes are arranged in a row, so that when conducting experiments on multiple liquid samples, the multiple liquid samples can be placed in corresponding sample holding tubes, and then the first and second conductive materials are energized to process the multiple samples, thereby improving the experimental efficiency of the electroporation tube.

[0009] In the above-mentioned multi-channel electric shock tube, there are multiple first conductive material pieces, and the first conductive material pieces are arranged in a one-to-one correspondence with the sample holding tubes.

[0010] There can be multiple first conductive material pieces, which can correspond one-to-one to the sample holding tubes and seal the first end openings of the corresponding sample holding tubes. At this time, the first conductive material piece can be directly embedded in the sample holding tube to seal the first end opening, thereby ensuring better experimental results.

[0011] In the above-mentioned multi-channel electric shock tube, the first conductive material piece is provided in a strip shape and is located below all the sample holding tubes and blocks the first end openings of the sample holding tubes.

[0012] The first conductive material piece adopts a long strip design, so that one first conductive material piece can seal all the sample holding tubes, thereby improving the convenience of sealing the sample holding tubes.

[0013] Furthermore, the first conductive material piece may be in the shape of a long strip.

[0014] In the above-mentioned multi-channel electric shock tube, there are multiple cover bodies, which are arranged one by one corresponding to the sample holding tubes. Two adjacent cover bodies are fixedly connected by a connecting strip, and the second conductive material piece is provided in each of the cover bodies.

[0015] By providing multiple cover bodies and connecting two adjacent cover bodies with connecting strips, while achieving separation of the cover bodies from each other, the multiple cover bodies can also be quickly and stably fastened together, thereby improving the convenience of using the multi-channel electric shock tube and enhancing experimental efficiency.

[0016] The connecting strip can be integrally formed with the cover body, or can be an independent component and connect multiple cover bodies through shape.

[0017] In the above-mentioned multi-channel electric shock tube, the cover body is provided with one and is in the shape of an elongated strip, and the second conductive material piece is provided in plurality and is located inside the cover body.

[0018] This structural arrangement enables the second conductive material piece to quickly and synchronously seal the second end opening of the sample holding tube as the cover is closed, thereby improving the sealing effect and facilitating rapid experiments on multiple sample holding tubes.

[0019] In the above-mentioned multi-channel electric shock tube, the cover body is provided with one and is in the shape of a long strip, the second conductive material piece is provided with one and is in the shape of a long strip, and the second conductive material piece is arranged in the cover body. After the cover body is closed, the second conductive material piece seals the second end opening of the sample holding tube.

[0020] The provision of the second strip-shaped conductive material piece allows the second end opening of the sample holding tube to be sealed synchronously and quickly during the covering process, thereby improving the covering efficiency and further improving the experimental efficiency.

[0021] In the above-mentioned multi-channel electric shock tube, the sample holding tube is made of elastic material, and the edge of the sample holding tube is squeezed and deformed when the second conductive material piece seals the second end opening of the sample holding tube.

[0022] Similarly, the sample holding tube is made of an elastic material, and the edges of the sample holding tube can be squeezed and deformed when the first conductive material seals the first end opening of the sample holding tube. Sample holding tubes are generally made of polymers such as plastic, silicone, and rubber. When using elastic materials such as silicone, rubber, and PVC, the main body of the sample holding tube can be thicker, while the first and second end openings can be provided with relatively thin sealing ribs. The relatively thin sealing ribs are easily deformed under pressure to ensure sealing, while the relatively thick main body can withstand pressure and maintain the shape of the sample holding tube.

[0023] By adopting elastic material, the second conductive material piece or the first conductive material piece can be squeezed and deformed when sealing the sample holding tube, thereby ensuring the sealing effect inside the sample holding tube and improving the stability of the experiment.

[0024] In the above-mentioned multi-channel electroporation tube, a plurality of sample holding tubes form a multi-unit sample holding tube, and a gap is provided between two adjacent sample holding tubes.

[0025] The multiple sample-holding tubes can be composed of four, six, or eight sample-holding tubes and can be manufactured using injection molding, which facilitates assembly and production, reducing production and installation costs. The multiple sample-holding tubes can also be connected by a connecting component having multiple insertion positions for inserting the multiple sample-holding tubes. Alternatively, the connecting component can be a portion of the first conductive material member, having multiple conductive protrusions corresponding one-to-one with the sample-holding tubes, and each sample-holding tube can be sleeved onto one of the protrusions to form a connection.

[0026] By providing a clearance gap, when the sample tube is slightly overfilled, the lid is pressed down to squeeze out the small amount of liquid. This squeezed-out liquid flows between two adjacent sample tubes, rather than into the adjacent tubes, thus preventing contact between adjacent sample tubes and affecting the experiment. Generally, a slightly overfilled tube is sufficient; any squeezed-out liquid will adhere to the tube opening and be difficult to flow into adjacent samples.

[0027] In the above-mentioned multi-channel electric shock tube, spacing grooves corresponding to the clearance gaps are opened in the transverse direction on the upper side of the second conductive material piece.

[0028] By providing spacing grooves that correspond one-to-one to the clearance gaps, the liquid squeezed out of the sample holding tube will flow out and adhere to the second conductive material piece. The spacing grooves can block the squeezed liquid from flowing to the vicinity of the adjacent sample holding tube opening, thereby improving the experimental efficiency while ensuring the stability of the experiment.

[0029] In the above-mentioned multi-channel electric shock tube, one or more clearance openings are opened at the upper end of the cover body, and all the clearance openings are distributed along the length direction of the cover body. When the cover body is closed, the second conductive material piece is located below the clearance openings.

[0030] The setting of the clearance port enables external current to be introduced into each sample holding tube when the second conductive material piece is electrically connected to the outside world, thereby improving the experimental efficiency of synchronous experiments in multiple sample holding tubes.

[0031] In the above-mentioned multi-channel electric shock tube, the multi-channel electric shock tube also includes a long strip-shaped box body, the multi-sample holding tube is arranged in the box body, a connection port is opened at the bottom of the box body, and a mounting base is clamped at the bottom of the box body. The above-mentioned first conductive material piece is installed in the mounting base, and the first conductive material piece is located below the connection port.

[0032] A mounting base is clamped at the bottom of the box body. By arranging the first conductive material member in the mounting base, the box body and the mounting base can press the first conductive material member onto the sample holding tube through the clamping force when they are installed, thereby improving the sealing of the lower end of the sample holding tube and ensuring the stability of the experiment.

[0033] In the above-mentioned multi-channel electric shock tube, a frame is connected to the box body, and the frame is provided with mounting holes arranged one-to-one corresponding to the sample holding tubes. The outer side walls of the sample holding tubes are provided with outwardly protruding limit stops. The sample holding tubes are inserted into the corresponding mounting holes and the lower end surface of the frame abuts against the upper end surface of the limit stop.

[0034] The setting of the frame makes it more convenient to arrange the sample holding tube in the box body, and the setting of the limit stop edge makes the first conductive material piece not only play the role of electrification, but also can press the limit stop edge of the sample holding tube onto the frame, thereby realizing the positioning of the sample holding tube and making the installation stability of the multi-channel electric shock tube higher.

[0035] In the above-mentioned multi-channel electroporation tube, the sample holding tubes are all hollow cylindrical, and two adjacent sample holding tubes are connected by a connecting component, and the upper end of the connecting component abuts against the lower end surface of the frame.

[0036] By setting the connecting components, the sample holding tube can be quickly installed in the box body, and the installation of the entire multi-channel electric shock tube is convenient.

[0037] In the above-mentioned multi-channel electroporation tube, the connecting component is arranged at the lower ends of the sample holding tubes and connects the lower ends of the sample holding tubes, and the above-mentioned clearance gap is formed between the upper ends of two adjacent sample holding tubes.

[0038] Because the lower ends of the sample-holding tubes are always in contact with the first conductive material, when liquid is injected into the corresponding sample-holding tube and slightly overflows, the liquid overflows from the upper connection opening when the sample-holding tube is pressed by the second conductive material. Therefore, by providing a clearance gap at the upper ends of the sample-holding tubes, the liquids in two adjacent sample-holding tubes do not interfere with each other. The positioning of the connecting components also improves the installation efficiency of multiple sample-holding tubes while ensuring experimental stability.

[0039] In the above-mentioned multi-channel electric shock tube, card interfaces can be opened on the side walls on both the front and rear sides of the box body, and the mounting base has elastic card blocks arranged in a one-to-one correspondence with the card interfaces. The elastic card blocks are embedded in the corresponding card interfaces and connect the mounting base to the box body.

[0040] In the above structure, the positions of the card interface and the elastic card block can also be swapped to achieve the same card connection function.

[0041] In the above-mentioned multi-channel electric shock tube, the elastic card blocks all have a clamping end, the elastic card blocks are located inside the box body and the clamping ends are clamped into the corresponding card interfaces from the inside of the box body, there is a gap between the elastic card blocks and the outer wall of the sample holding tube, and the elastic card blocks are located outside the connecting component.

[0042] The elastic card block is inserted into the corresponding card interface from the inside of the box body. During the experiment, the multi-channel electric shock tube needs to be held in hand when pouring the liquid to be electroporated. Therefore, by inserting it from the inside of the box body, the elastic card block is stably set in the box body and does not protrude from the box body, avoiding accidental touching of the elastic card block and causing the box body to separate from the installation box, thereby improving the stability of the use of the multi-channel electric shock tube. The elastic card block is located on the outside of the connecting block, making the loading and unloading of the installation box more convenient and quick, further improving the convenience of installation and use.

[0043] In the above-mentioned multi-channel electric shock tube, the clamping end is located in the clamping interface, and there is a distance between the upper end of the clamping end and the mouth wall of the clamping interface, and the lower end surface of the clamping end abuts against the mouth wall of the clamping interface.

[0044] The setting of the card end ensures that the entire elastic card block is located within the outer wall of the box body after installation, so that accidental touching will not occur during use, which improves the convenience of using the electric shock tube. The setting of the spacing allows the card end to have space to move upward in the card interface, further facilitating the installation of the base frame.

[0045] In the above-mentioned multi-channel electric shock tube, the outer side wall of the mounting base is inwardly recessed to form a groove corresponding to the elastic block. The groove is arranged vertically and the lower end of the box body is arranged opposite to the groove.

[0046] The setting of the groove realizes the longitudinal displacement of the elastic card block, so that the elastic card block only needs the lower end surface of the card end to rest against the wall of the card interface, and no other limiting components are set below, which improves the convenience of installation.

[0047] In the aforementioned multi-channel shock tube, a rotatable connection mechanism is provided between the cover and the box body. The rotatable connection mechanism comprises a hinged column disposed on one side of the cover body, a protrusion on the outer wall of the box body forming an upper limit block and a lower limit block, a notch formed in the cover body for the upper limit block to pass through, and a plurality of axially arranged positioning blocks on the hinged column, each of which is disposed at one end of the upper limit block or the lower limit block.

[0048] The setting of this structure enables a detachable hinge structure to be adopted between the cover body and the box body. In order to improve the stability of the connection, the upper limit block and the lower limit block are set to improve the stability of the hinge between the cover body and the box body.

[0049] In addition, the hinge shaft is arranged outside the outer wall of the box body, so that when the cover body is opened, it will not block the communication port at the upper end of the sample holding tube in the box body, making it easier to inject liquid into the sample holding tube and improving the convenience of using the sample holding tube.

[0050] In the aforementioned multi-channel electroporation tube, the rotatable connection mechanism includes a flexible connecting strip that helps maintain the connection between the cover and the box body when the cover is opened and closed. The flexible connecting strip can be integrally formed with the cover or box body. A switch latch is provided on the other side wall of the box body and the cover opposite the rotatable connection mechanism. When the switch latch is opened, the cover can be opened to allow the addition of a liquid sample. After the addition of the sample is completed, the switch latch is closed to close the cover and secure it to the box body. At this point, the second conductive material member seals the second end opening of the sample-holding tube, allowing the sample therein to undergo an electroporation experiment. After the electroporation experiment is completed, the switch latch can be opened to allow the liquid sample to be removed.

[0051] The present invention also provides a method for batch processing of multiple sample cell electroporation. Using the multi-channel electroporation tube of the present invention, liquid samples are added while the lid is open, typically using a multi-channel pipette to simultaneously add multiple samples. The samples contain cells and substances to be injected into the cells. During sample addition, the volume of the liquid sample is slightly larger than the volume within the sample-holding tube, resulting in a slightly curved convex surface at the top of the liquid. After sample addition, the lid is closed, and a second conductive material member in the lid contacts the liquid and presses against the second end opening of the sample-holding tube, sealing the sample-holding tube. After the second end opening is sealed, a small amount of squeezed-out liquid flows into a clearance gap near the second end opening. A cell electroporation instrument is then used to deliver electric pulses to the multi-channel electroporation tube to conduct the electroporation experiment. After electroporation is completed, the lid is opened and the samples are removed from the sample-holding tube, typically using a multi-channel pipette. The removed cell samples can be cultured in a conventional cell culture plate with culture wells arranged in rows and columns, or in other types of culture plates.

[0052] The cell culture plate and multi-channel pipette are both existing structures.

[0053] Compared with the existing technology, this multi-channel electric shock tube has the following advantages:

[0054] 1. By fixing multiple sample holding tubes in a row, when conducting experiments on multiple liquid samples, multiple liquid samples can be placed in corresponding sample holding tubes through the arrangement of multiple sample holding tubes, and batch processing of multiple samples can be achieved by simply energizing the first conductive material member and the second conductive material member, thereby achieving the purpose of improving the experimental efficiency of the electric shock tube.

[0055] 2. By sealing the opening of the sample tube with the first and second conductive materials, bubbles generated during electrochemical cell transfer can be compressed, thereby improving electroporation efficiency and cell survival rate during the electroporation experiment. The multi-channel electroporation tube has a reliable structure, is easy to operate, and can improve sample experimental results.

[0056] 3. A mounting base is snap-fitted to the bottom of the box body. By placing the first conductive material member inside the mounting base, the box body and the mounting base can be mounted such that the first conductive material member is pressed against the sample holding tube by the clamping force of the snap-fit ​​connection, thereby improving the sealing of the lower end of the sample holding tube and ensuring the stability of the experimental device. BRIEF DESCRIPTION OF THE DRAWINGS

[0057] Figure 1 It is a structural diagram of the first embodiment.

[0058] Figure 2 It is a top view of the first embodiment.

[0059] Figure 3 yes Figure 2 Cross-sectional view of AA in the figure.

[0060] Figure 4 It is a structural diagram of the fourth embodiment.

[0061] Figure 5 It is a top view of the fourth embodiment.

[0062] Figure 6 yes Figure 5 Cross-sectional view of the BB.

[0063] Figure 7 yes Figure 5 Cross-sectional view of CC.

[0064] Figure 8 yes Figure 5 Cross-sectional view of DD.

[0065] Figure 9 Schematic diagram of the structure of the multi-sample holding tube in the fourth embodiment.

[0066] Figure 10 Schematic diagram of the structure of the cover body in the fourth embodiment.

[0067] Figure 11 This is a schematic structural diagram of the assembled cover and the second conductive material member in the fourth embodiment.

[0068] In the figure, 1. box body; 11. opening; 12. connecting port; 13. card interface; 14. upper limit block; 15. lower limit block; 16. buckle block; 2. mounting base; 21. first conductive material member; 22. elastic card block; 22a. card connection end; 22b. spacing; 23. interval; 24. groove; 33. limit stop edge; 34. connecting component; 4. cover body; 41. second conductive material member; 41a. spacing groove; 42. clearance port; 43. hinged column; 43a. positioning block; 44. notch; 45. buckle plate; 45a. buckle interface; 5. clearance; 6. frame; 61. mounting hole; 7. multi-sample holding tube; 8. sample holding tube; 81. first end opening; 82. second end opening; 9. connecting strip. DETAILED DESCRIPTION

[0069] The following are specific embodiments of the present invention and the accompanying drawings to further describe the technical solutions of the present invention, but the present invention is not limited to these embodiments.

[0070] Example 1

[0071] like Figure 1-3 As shown, the multi-channel electric shock tube includes a plurality of sample holding tubes 8 made of insulating material, and the plurality of sample holding tubes 8 are fixed in a row. The sample holding tubes 8 have a first end opening 81 and a second end opening 82. The multi-channel electric shock tube also includes a first conductive material piece 21, a cover body 4, and a second conductive material piece 41. The first conductive material piece 21 blocks the first end opening 81 of the sample holding tube 8. When the cover body 4 is covered on the sample holding tube 8, the second conductive material piece 41 blocks the second end opening 82 of the sample holding tube 8. There are a plurality of first conductive material pieces 21, and the first conductive material pieces 21 are arranged in a one-to-one correspondence with the sample holding tubes 8.

[0072] By setting up multiple sample holding tubes 8, the sample holding tubes 8 are filled with liquid samples containing cells and substances to be injected into the cells. The first end opening 81 of the sample holding tube 8 is blocked by the first conductive material piece 21. The multi-channel electric shock tube is also provided with a cover body 4. The second end opening 82 of the cover body 4 is blocked by the second conductive material piece 41. The multiple sample holding tubes 8 are fixed in a row, so that when conducting experiments on multiple liquid samples, multiple liquid samples are placed in the corresponding sample holding tubes 8, and then the first conductive material piece 21 and the second conductive material piece 41 are energized to achieve the purpose of processing multiple samples for electroporation and improving the experimental efficiency.

[0073] like Figure 1 and Figure 3As shown, the sample holding tube 8 is made of elastic material. When the second conductive material piece 41 blocks the second end opening 82 of the sample holding tube 8, the edge of the sample holding tube 8 is squeezed and deformed. There are multiple cover bodies 4, and they are arranged one by one corresponding to the sample holding tubes 8. The adjacent two cover bodies 4 are fixedly connected by a connecting strip 9, and the second conductive material piece 41 is provided in each cover body 4.

[0074] Example 2

[0075] The content of this embodiment is basically the same as that of the first embodiment, except that: one first conductive material piece 21 is provided and is in the shape of an elongated strip. The first conductive material piece 21 is located below all sample holding tubes 8 and blocks the first end opening 81 of the sample holding tube 8 .

[0076] Example 3

[0077] The content of this embodiment is basically the same as that of the first embodiment, except that: there is one cover 4 in a strip shape, there are multiple second conductive material pieces 41 , and the second conductive material pieces 41 are located inside the cover 4 .

[0078] Example 4

[0079] The content of this embodiment is basically the same as that of the first embodiment, except that: Figure 4-6 As shown, the cover body 4 is provided with a strip-shaped second conductive material piece 41, which is provided with a strip-shaped second conductive material piece 41. The second conductive material piece 41 is arranged in the cover body 4. After the cover body 4 is closed, the second conductive material piece 41 blocks the second end opening 82 of the sample holding tube 8. The multiple sample holding tubes 8 form a multi-unit sample holding tube 7. There is a clearance gap 5 between two adjacent sample holding tubes 8. The multi-unit sample holding tube 7 is injection molded. The second conductive material piece 41 is provided with a plurality of slots 5 corresponding to the clearance gaps 5 on the horizontal direction. The cover body 4 has a spacing groove 41a, and a clearance opening 42 is provided at the upper end of the cover body 4. All the clearance openings 42 are distributed along the length direction of the cover body 4. The second conductive material piece 41 is located below the clearance opening 42. The multi-channel electric shock tube also includes a long strip box body 1, and the multi-sample holding tube 7 is arranged in the box body 1. The bottom of the box body 1 is provided with a connecting port 12. The bottom of the box body 1 is clamped with a mounting base 2, and the mounting base 2 is installed with the above-mentioned first conductive material piece 21. The first conductive material piece 21 is located below the connecting port 12.

[0080] like Figure 4-9As shown, a frame 6 is connected to the box body 1. The frame 6 is provided with mounting holes 61 corresponding to the sample holding tubes 8. The outer side walls of the sample holding tubes 8 are provided with outwardly protruding limit stops 33. The sample holding tubes 8 are inserted into the corresponding mounting holes 61, and the lower end surface of the frame 6 abuts against the upper end surface of the limit stops 33. Two adjacent sample holding tubes 8 are connected by a connecting component 34. The connecting component 34 is integrally formed with the sample holding tubes 8. The upper end of the connecting component 34 abuts against the lower end surface of the frame 6. The connecting component 34 is provided at the lower end of the sample holding tubes 8 and connects the lower ends of the sample holding tubes 8, forming the above-mentioned clearance gap 5 between the upper ends of the two adjacent sample holding tubes 8.

[0081] like Figure 6-10 As shown, the side walls on both the front and rear sides of the box body 1 are provided with card interfaces 13, and the mounting base 2 is provided with elastic card blocks 22 arranged one-to-one corresponding to the card interfaces 13. The elastic card blocks 22 are embedded in the corresponding card interfaces 13 and connect the mounting base 2 to the box body 1. The elastic card blocks 22 have a card connection end 22a. The elastic card blocks 22 are located on the inner side of the box body 1 and the card connection end 22a is inserted into the corresponding card interface 13 from the inner side of the box body 1. The elastic card blocks 22 are connected to the outer wall of the sample holding tube 8. There is a gap 23 between them, the elastic card block 22 is located on the outside of the connecting part 34, the card end 22a is located in the card interface 13, and there is a gap 22b between the upper end of the card end 22a and the mouth wall of the card interface 13, and the lower end surface of the card end 22a is against the mouth wall of the card interface 13. The outer wall of the mounting base 2 is recessed inward to form a groove 24 corresponding to the elastic card block 22. The groove 24 is arranged vertically and the lower end of the box body 1 is opposite to the groove 24.

[0082] like Figure 4 and Figure 11 As shown, a rotatable connection mechanism is provided between the lid 4 and the box body 1. This rotatable connection structure includes a hinge post 43 provided on one side of the lid. A protrusion on the outer wall of the box body 1 forms an upper stopper 14 and a lower stopper 15. The lid 4 is provided with a notch 44 for the upper stopper 14 to pass through. The hinge post 43 is provided with multiple axially arranged positioning blocks 43a, each positioned at one end of the upper stopper 14 or the lower stopper 15. A snap plate 45 is provided on the other side of the lid 4, with a snap interface 45a formed on the snap plate 45. The outer wall of the box body 1 protrudes outward to form a snap block 16 that fits into the snap interface 45a. The provision of the snap plate 45 and the snap interface 45a further enhances the stability of the connection between the lid 4 and the box body 1.

[0083] Example 5

[0084] The content of this embodiment is basically the same as that of the first embodiment, except that:

[0085] The rotatable connection mechanism includes a flexible connecting strip that helps maintain the connection between the cover 4 and the box body 1 when the cover 4 is opened and closed. The flexible connecting strip can be integrally formed with the cover 4 or the box body 1. A switch latch is provided on the side wall of the box body 1 and the cover 4 opposite the rotatable connection mechanism. When the switch latch is opened, the cover 4 is opened to allow the addition of a liquid sample. After the sample is added, the switch latch is closed to close the cover 4 and secure it to the box body 1. At this time, the second conductive material member 41 seals the second end opening 82 of the sample holding tube 8, allowing the sample therein to undergo an electroporation experiment. After the electroporation experiment is completed, the switch latch can be opened to remove the liquid sample.

[0086] The specific embodiments described herein are merely illustrative of the spirit of the present invention. Persons skilled in the art may make various modifications, additions, or substitutions to the described specific embodiments without departing from the spirit of the present invention or exceeding the scope of the appended claims.

Claims

1. A multi-channel electric shock tube, comprising a plurality of sample holding tubes (8) made of insulating material, characterized in that: A plurality of sample holding tubes (8) are fixed in a row, wherein the sample holding tubes (8) have a first end opening (81) and a second end opening (82). The multi-channel electric shock tube further comprises a first conductive material piece (21), a cover body (4) and a second conductive material piece (41). The first conductive material piece (21) blocks the first end opening (81) of the sample holding tube (8), and when the cover body (4) is covered on the sample holding tube (8), the second conductive material piece (41) blocks the second end opening (82) of the sample holding tube (8).

2. The multi-channel electric shock tube according to claim 1, characterized in that: There are a plurality of first conductive material pieces (21), and the first conductive material pieces (21) are arranged in a one-to-one correspondence with the sample holding tubes (8).

3. The multi-channel electric shock tube according to claim 1 or 2, characterized in that: The first conductive material piece (21) is provided in a strip shape and is located below all sample holding tubes (8) and blocks the first end opening (81) of the sample holding tube (8).

4. The multi-channel electric shock tube according to claim 1 or 2, characterized in that: There are multiple covers (4) and they are arranged one by one corresponding to the sample holding tubes (8). Two adjacent covers (4) are fixedly connected by a connecting strip (9). A second conductive material piece (41) is provided in each of the covers (4).

5. The multi-channel electric shock tube according to claim 1 or 2, characterized in that: The cover body (4) is provided with one and is in the shape of an elongated strip, a plurality of second conductive material pieces (41) are provided, and the second conductive material pieces (41) are located inside the cover body (4).

6. The multi-channel electric shock tube according to claim 1 or 2, characterized in that: The cover (4) is provided with a strip-shaped second conductive material piece (41), which is provided with a strip-shaped second conductive material piece (41). The second conductive material piece (41) is arranged inside the cover (4). When the cover (4) is closed, the second conductive material piece (41) blocks the second end opening (82) of the sample holding tube (8).

7. The multi-channel electric shock tube according to claim 1 or 2, characterized in that: The sample holding tube (8) is made of elastic material, and the edge of the sample holding tube (8) is squeezed and deformed when the second conductive material piece (41) seals the second end opening (82) of the sample holding tube (8).

8. The multi-channel electric shock tube according to claim 6, characterized in that: A plurality of sample holding tubes (8) form a multi-unit sample holding tube (7), and a clearance gap (5) is provided between two adjacent sample holding tubes (8).

9. The multi-channel electric shock tube according to claim 8, characterized in that: The second conductive material piece (41) is provided with spacing grooves (41a) arranged in a one-to-one correspondence with the clearance gaps (5) along the transverse direction.

10. The multi-channel electric shock tube according to claim 8, characterized in that: The multi-channel electric shock tube further comprises a long strip-shaped box body (1), the multi-sample holding tube (7) is arranged in the box body (1), a connection port (12) is provided at the bottom of the box body (1), a mounting base (2) is clamped at the bottom of the box body (1), the first conductive material member (21) is installed in the mounting base (2), and the first conductive material member (21) is located below the connection port (12).

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