Flower forcing liquid for babysbreath cut flowers and preparation method of flower forcing liquid

Abstract

The invention belongs to the technical field of fresh cut flower preservation, and discloses babysbreath cut flower forcing liquid and a preparation method thereof. The catalytic liquid is prepared from, by mass, 0.6%-1.6% of 8-hydroxyquinoline, 1.0%-1.8% of citric acid, 80-150 mg / L of gibberellin, 0.4%-1.2% of ascorbic acid, 60-100 mg / L of silver thiosulfate and 0.4%-1.5% of aluminum sulfate. The concentration of Tween-20 is 0.2%-0.5% by volume percent, so that the synchronization of rapid blooming of lower-layer flower buds and anti-aging of upper-layer flowers of the babysbreath cut flowers is realized, the blooming time of the lower-layer flower buds is shortened, and the qualification rate of commodities and the vase life are remarkably improved. The method is suitable for the whole process of grading, pretreatment, transportation and bottle insertion after harvesting of the cut babysbreath flowers. The invention further provides an efficient and stable flower forcing liquid preparation process, component stability and permeability are guaranteed, working efficiency and unit area stubbles and yield are improved so as to adapt to market changes, and the flower forcing liquid has good development prospects in production, popularization and application of babysbreath cut flowers.

Description

Technical Field

[0001] The present invention belongs to the technical field of post-harvest cut flower preservation, specifically relating to a formula and preparation method for a post-harvest flower-inducing liquid to treat the asynchronous opening of the panicle cymes of Gypsophila paniculata and the browning of the upper flowers. Through sugar source gradient regulation, dual-pathway ethylene-oxidation inhibition, and the synergistic effects of plant growth regulators, rapid opening of the lower buds and anti-aging of the upper flowers are synchronized, significantly improving the product's pass rate and vase life. This invention is applicable to the entire post-harvest grading, pretreatment, transportation, and vase placement process for Gypsophila paniculata cut flowers, particularly for Yunnan's major cultivars such as "Yilos," "Xueshan," "Yunxing Series," and "Xingbai." Background Art

[0002] Bud-opening solution: A type of cut flower preservative, bud-opening solution regulates the physiological and biochemical metabolism of cut flowers to artificially regulate the aging process, reduce circulation losses, and improve their quality or ornamental value. It primarily forcibly promotes the opening of cut flowers harvested during the bud stage. Bud-opening solution treatment is typically performed by producers or aggregators before sale. It is a method of artificially inducing bud opening. It is commonly used in cool weather, when flowering is slow and fails to meet target; to achieve desired yields and profits; and when buds have difficulty opening after long-term storage or long-distance or prolonged transportation.

[0003] Flower-inducing solutions are formulated based on the characteristics of different cut flowers and are generally specific and cannot be mixed. Flower-inducing solutions typically contain carbohydrates, ethylene inhibitors, fungicides, pH regulators, growth regulators, antioxidants, mineral elements, and surfactants. The specific composition varies depending on the flower type and the stage of processing. Carbohydrates such as sucrose, fructose, glucose, and trehalose provide energy and respiratory substrates for cut flowers, promoting bud expansion and opening, maintaining normal cellular osmotic pressure, and delaying petal wilting. The concentration needs to be adjusted at different stages of the process. For postharvest processing, 4% to 8% sucrose is recommended, as higher concentrations can easily breed microorganisms. Ethylene inhibitors include silver thiosulfate (STS), 1-methylcyclopropene (1-MCP), and aminooxyacetic acid (AOA). These primarily block ethylene receptors, delaying flower aging, and are suitable for ethylene-sensitive cut flowers (such as baby's breath, carnations, and delphiniums). Fungicides can effectively inhibit bacterial growth that can block the water supply ducts of cut flowers, extending their water absorption and vase life. Commonly used fungicides include 8-hydroxyquinoline (8-HQ), 8-hydroxyquinoline sulfate (8-HQS), silver nitrate (AgNO3), thiabendazole (TBZ), and quaternary ammonium salts. Fungicides can have toxic side effects on some cut flowers, causing leaf burns or chlorosis, bleaching or browning of stems, and yellowing of white flowers. pH regulators (such as citric acid and potassium dihydrogen phosphate) lower the solution pH (usually to 4.5-5.0), enhancing fungicidal efficacy and promoting water absorption. Growth regulators regulate the flowering process, promoting synchronous bud opening, delaying senescence, and maintaining petal cell activity. Gibberellic acid (GA3) and phenylacetic acid (PAA) promote stem elongation and bud opening. Cytokinins (such as 6-BA) and salicylic acid (SA) delay senescence and, when used in conjunction with gibberellins, accelerate flowering. Thidiazuron (TDZ) promotes cell division and is suitable for gypsophila with thin stems. Antioxidants include vitamin C (ascorbic acid) and vitamin E, which reduce petal browning and slow lipid peroxidation in cell membranes. Mineral elements maintain cell membrane stability, reduce water loss, and strengthen stems to prevent bending. Commonly used are calcium salts (such as calcium nitrate and calcium chloride) and aluminum salts (such as aluminum sulfate). Surfactants increase solution permeability and promote absorption of ingredients, such as polyoxyethylene lauryl ether, Triton X-100, and Tween-20.

[0004] At present, the number of patents for cut flower inducing liquids published by the State Intellectual Property Office of the People's Republic of China is very few. CN113197198A discloses a fresh cut flower inducing liquid, which is made by dissolving citric acid, Tween-20, 6-benzylaminopurine, sucrose and sodium hydroxide in water in proportion. The fresh cut flower inducing liquid prepared by this method, although it can make cut flowers bloom quickly, lacks the fresh-keeping effect of cut flowers (such as lacking bactericides and ethylene inhibitors), reduces the vase life of cut flowers, and even causes damage to cut flowers (such as sodium hydroxide), particularly to some ethylene-sensitive cut flowers. In addition, the number of patents for inducing liquids and the number of flowers used are few, making it difficult to meet the huge demand and personalized formulation requirements of consumers for various cut flowers. In addition, due to the different biological and physiological characteristics (flower shape, amount of flowers, color, texture, etc.) of various cut flowers after harvest, a cut flower inducing liquid technology is difficult to be applied to all cut flower materials. Therefore, exploring different special inducing liquids for cut flowers is a very necessary and urgent problem to be solved.

[0005] Gypsophila paniculata L., formerly known as cone stone flower, also known as cone-flowered dianthus, cone-flowered dianthus, silk dianthus, June snow, and rosy grass, is a perennial herbaceous plant in the genus Gypsophila, Caryophyllaceae. It is also the only species in this genus used as a cut flower. Gypsophila paniculata is known for its small, few leaves, numerous, slender flower branches, abundant, fluffy, and small, pure white flowers. Its clusters create a striking effect, resembling star-like dots, creating a three-dimensional effect and adding a hazy beauty to bouquets. It has become one of the world's most widely used flower arrangements and one of the top ten best-selling cut flowers worldwide, widely used in a variety of bouquets and floral arrangements. However, its inflorescence is a multi-branched, conical cyme with numerous, small flowers, which bloom initially at the top and then gradually spread downward. At the same time, due to differences in maturity, light exposure, flower branch layers, and primary and secondary buds, flowers do not open simultaneously. The main flowers, which are located near the upper layers and are rich in nutrients, will open first, followed by the delicate flowers in the middle and lower layers. The market often demands an inflorescence opening rate of at least 80% for Gypsophila cut flowers, but due to its physiological characteristics, the top florets open first, while the lower buds lag behind. The upper flowers, due to their early maturity, strong light exposure, and nutritional advantage, open first, then quickly brown and wither, becoming dried flowers. The lower buds have not yet reached the opening standard, resulting in a commercial yield of less than 70%, ultimately affecting the quality of the entire cut flower. At present, there are many reports on the preservation of domestic babysbream cut flowers. CN1602689A discloses a pre-treatment liquid for babysbream cut flowers. The pre-treatment liquid containing 6-BA and vitamin E can delay wilting, but does not solve the problem of opening synchronism. Similarly, CN101766159A discloses a preservative for babysbream, which is composed of silver nitrate, sucrose, isoascorbic acid and water. It lacks plant growth regulators (such as gibberellins) to promote the opening of flower buds. It is also for solving the problem of opening synchronism. In addition, high-concentration chemical fungicides (such as silver nitrate) residues pollute water sources. More in-depth or more comprehensive and systematic research has not been reported yet. There is no preservative for babysbream cut flowers used as flower-forcing liquid to appear. Therefore, it is particularly important to develop a rapid opening of lower buds and the anti-aging synchronization of upper flowers, significantly improve the qualified rate of goods and the special flower-forcing liquid and systematic application method of babysbream cut flowers. Summary of the Invention

[0006] To address the shortcomings of existing technologies, the present invention provides a flower-inducing liquid specifically for baby's breath cut flowers. This liquid synchronizes the rapid opening of lower buds with the aging of upper flowers, shortening the opening time of lower buds, significantly improving product quality, and extending vase shelf life. The present invention also provides an efficient and stable preparation process for the liquid, ensuring ingredient stability and permeability.

[0007] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0008] The invention provides a baby's breath cut flower flowering inducing liquid, which is characterized in that the flowering inducing liquid is prepared by dissolving 8-hydroxyquinoline (8-HQ), citric acid, gibberellin, ascorbic acid, silver thiosulfate and Tween-20 in water in proportion.

[0009] The dosage of each component in the flower-inducing liquid is as follows: the concentrations of the following components, measured by weight / volume percentage, are: 0.6%-1.6% of 8-hydroxyquinoline, 1.0%-2.2% of citric acid, 80-150 mg / L of gibberellins, 0.4%-1.5% of ascorbic acid (VC), 60-100 mg / L of silver thiosulfate, and 0.4%-1.5% of aluminum sulfate; and the concentration of Tween-20, measured by volume percentage, is 0.2%-0.5%.

[0010] The baby's breath cut flower flowering inducing liquid also includes 50 to 100 μmol / L of sodium nitroprusside (SNP) in volume percentage. Sodium nitroprusside can inhibit the activities of ACC synthase and ACC oxidase, block ethylene signal transduction, and simultaneously scavenge reactive oxygen free radicals (ROS), reduce malondialdehyde (MDA) content, maintain cell membrane integrity, and be used in combination with 8-hydroxyquinoline (8-HQ) to enhance the inhibitory effect on stem vessel clogging bacteria.

[0011] The baby's breath cut flower flowering-inducing liquid also includes 10 to 100 mg / L of cytokinin (6-benzylpurine, 6-BA) in mass percentage. The addition of cytokinin (6-BA) can inhibit the expression of the SAG12 gene in petals, delay aging, and synergistically improve the uniformity of inflorescences with GA3.

[0012] The baby's breath cut flower flowering-inducing liquid also includes 0.6% to 1.2% vitamin E and 0.05% to 0.1% vitamin B2 in percentage by weight. The addition of vitamin E can remove ROS, reduce MDA content, and protect cell membrane integrity; vitamin B2 enhances cell membrane stability, reduces water loss, and assists in anti-aging.

[0013] Specifically, the dosage of each component in the flower-inducing liquid is as follows: the concentrations of the following components, measured by weight / volume percentage, are: 8-hydroxyquinoline 1.0%, citric acid 1.5%, gibberellin 100 mg / L, ascorbic acid 0.8%, silver thiosulfate 80 mg / L, aluminum sulfate 0.8%, sodium nitroprusside 50 μmol / L, 6-BA 30 mg / L, vitamin E 0.6% and vitamin B2 0.05%; and the concentration of Tween-20, measured by volume percentage, is 0.3%.

[0014] The present invention also provides a method for preparing the above-mentioned cut flower inducing liquid, which is characterized in that the liquid is prepared according to the following steps (see the step-by-step process flow chart Figure 1 ):

[0015] S1 raw materials weighed:

[0016] Accurately weigh each component raw material according to the ratio;

[0017] S2 raw material pretreatment:

[0018] Citric acid was dissolved in 50°C deionized water, magnetically stirred (300 rpm) until completely dissolved, and the pH was adjusted to 4.8±0.2 using a pH meter; 8-hydroxyquinoline was dissolved in 10% ethanol solution (volume ratio) and ultrasonically treated (40 kHz, 10 minutes) to form a uniform suspension; gibberellin and 6-benzylpurine were added to 50 mL of anhydrous ethanol and stirred to fully dissolve; sodium thiosulfate and silver nitrate were separately dissolved in 50 mL of distilled water, and then the silver nitrate solution was slowly poured into the sodium thiosulfate while stirring to allow the two solutions to fully dissolve each other, thereby obtaining silver thiosulfate; sodium nitroprusside and ascorbic acid were dissolved in 50 mL of 4°C deionized water in the dark and stored in a brown glass bottle to prevent photolysis failure.

[0019] S3 Gradient Blending:

[0020] To the citric acid solution, add the mixed ethanol solution of GA3 and 6-BA in sequence, and stir with constant temperature magnetic stirring (50°C, 200 rpm) until completely dissolved; slowly add the ethanol suspension of 8-hydroxyquinoline, and stir for 15 minutes in the dark to prevent photolysis; add the mixed solution of sodium nitroprusside and ascorbic acid in the dark at low temperature, and stir thoroughly to mix.

[0021] S4 anti-aging system construction:

[0022] Add freshly prepared STS solution (stock concentration 1 mM, final concentration 80 mg / L) in the dark to prevent oxidation and precipitation of silver ions; add vitamin E, vitamin B2, aluminum sulfate and Tween-20, dilute to the target volume with deionized water, and homogenize with a vortex mixer (2000 rpm) for 5 minutes.

[0023] S5 Filtration and Packaging:

[0024] The product was filtered under pressure (pressure 0.3 MPa) through a 0.22 μm nylon filter membrane to remove impurities and undissolved particles; the product was dispensed into brown light-proof glass bottles, sealed with nitrogen, and stored at 4°C.

[0025] Another aspect of the present invention provides a method for using the above-mentioned baby's breath cut flower flowering inducing liquid, characterized in that 4% to 8% of sucrose / glucose is added to the flowering inducing liquid before use, and then the cut flowers are treated.

[0026] On the other hand, the present invention further provides steps for using the above-mentioned baby's breath cut flower inducing liquid, comprising the following steps: selecting baby's breath with a branch length of about 70 to 120 cm and a flower opening degree of 3% to 35%, cutting the branches evenly at the base of the stems with sharp scissors, and placing the branches in a container filled with clean water as soon as possible after cutting; thoroughly cleaning and pre-cooling the branches, removing leaves and side branches and damaged flowers at the bottom third of the branches, retaining relatively complete and well-developed flowers and an appropriate amount of leaves at the top, trimming the branches neatly according to the length and thickness of the flower stems, ensuring that the inflorescences are upward and uniformly arranged; then simply tying and trimming the bases of the baby's breath branches, cutting the ends obliquely in water, and immediately placing the branches in the inducing liquid to a depth of 5 to 20 cm, ensuring that the lower ends of all stems are immersed in the inducing liquid.

[0027] According to a preferred embodiment, the steps of using the baby's breath cut flower flowering inducing liquid, the application of the baby's breath cut flower flowering inducing liquid in the preservation of baby's breath cut flowers, the environmental temperature of the flowering inducing room where the baby's breath flowering inducing liquid technology is located is maintained at 15-30°C, the relative humidity is 60%-85%, the light intensity is 1000-3000 lux, direct sunlight and poor ventilation are avoided, the flowering time is 3-10 days, and ensure that there is about 3L of flowering inducing liquid in the flowering inducing container to ensure that the entire flowering inducing process is continuous and will not dehydrate.

[0028] Among the ingredients in the above formula, sucrose provides an energy source for cut flowers, promoting bud expansion and opening; it also maintains cellular osmotic pressure and delays petal wilting. A gradient replenishes the carbon source needed for bud expansion: low concentrations (4%-6%) prevent osmotic imbalance in leaves, while high concentrations (6%-8%) promote expansion of lower buds. However, excessive concentrations can easily breed microorganisms, and improper dosage can cause sugar damage, leading to flower discoloration and leaf burns. 8-Hydroxyquinoline is the most commonly used broad-spectrum fungicide in preservatives. It kills bacteria by removing copper and iron ions from bacteria. Using 8-Hydroxyquinoline in preservatives inactivates tannins produced at the base of cut flower stems, thereby inhibiting microbial growth and preventing stem duct blockage. In preservatives, 8-Hydroxyquinoline is often used in combination with citric acid. Citric acid, the most widely used organic acid in preservatives, lowers the pH value and maintains an acidic environment. Citric acid not only dissolves 8-hydroxyquinoline but also fully dissolves the other ingredients. Gibberellic acid is a plant growth regulator that promotes lower flower stem elongation and bud opening, shortening the time it takes for lower buds to open. 6-Benzylpurine is also a plant growth regulator that delays senescence and synergistically promotes flowering. Combined with GA3, it improves inflorescence uniformity, prevents yellowing of stems and leaves, promotes water absorption, reduces the sensitivity of cut flowers, and inhibits ethylene action. Ascorbic acid (vitamin C) is an antioxidant that reduces petal browning and delays lipid peroxidation in cell membranes. Silver thiosulfate (the reaction product of sodium thiosulfate and silver nitrate) is an ethylene inhibitor, blocking ethylene synthesis or signaling, inhibiting ethylene release from upper flowers. Combined with vitamin E as an antioxidant, it significantly reduces browning and is also a fungicide. Vitamin E is also an antioxidant that reduces MDA content in petals and protects cell membrane integrity. Aluminum sulfate maintains cell membrane stability, reduces water loss, strengthens stems and prevents bending, lowers pH, and inhibits bacteria. Sodium nitroprusside inhibits the activity of ACC synthase and ACC oxidase, blocking ethylene signaling. It also scavenges reactive oxygen species (ROS), reduces malondialdehyde (MDA) levels, and maintains cell membrane integrity. Combined with 8-hydroxyquinoline (8-HQ), it enhances its inhibitory effect against stem-clogging bacteria. Vitamin B2 enhances cell membrane stability and reduces water loss. Combined with sucrose, it improves freshness preservation. Tween-20 is a surfactant that reduces surface tension and promotes water absorption by the stem.

[0029] The catalytic liquid of the present invention is based on the flowering and aging physiological characteristics of baby's breath, with ethylene inhibitors, fungicides, growth regulators, antioxidants, etc. as basic raw materials. By optimizing the sugar concentration gradient and introducing the synergistic effect of gibberellins (GA3) and 6-benzylpurine (6-BA), the lower buds are accelerated to open. At the same time, silver thiosulfate (STS), sodium nitroprusside (SNP) and vitamin E are used to inhibit the release of ethylene and oxidative damage in the upper flowers, as well as to regulate the pH value in the plant body, antagonize the effects of aging hormones, sterilize or antibacterial, supplement sugar sources, improve water balance, etc., to achieve the rapid opening of the lower buds and the synchronization of the anti-aging of the upper flowers, significantly improving the product qualification rate and bottle life. The present invention systematically solves the problem of asynchronous opening and quality decline of baby's breath cut flowers after harvest. Experimental data show that the product qualification rate is increased to 92%, with significant industrial application value. The present invention is applicable to the whole process of grading, pretreatment, transportation and bottle insertion of baby's breath cut flowers after harvest. In addition, the present invention adopts step-by-step dissolution and filtration technology to ensure the stability and permeability of the ingredients through precise ingredient ratio and preparation process optimization. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 : Flow chart of the process for preparing flower-inducing liquid. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0032] Example 1

[0033] The formula and dosage of the flower-inducing liquid with a volume of 1L: 8-hydroxyquinoline 10g, citric acid 15g, gibberellin 100mg, ascorbic acid 8g, silver thiosulfate 80mg, aluminum sulfate 8g, sodium nitroprusside 50μmol, 6-BA 30mg, vitamin E 6g, vitamin B2 0.5g, Tween-20 3mL.

[0034] And prepare it according to the following steps:

[0035] S1 raw materials weighed:

[0036] Accurately weigh each component raw material according to the ratio;

[0037] S2 raw material pretreatment:

[0038] Citric acid was dissolved in 50°C deionized water, magnetically stirred (300 rpm) until completely dissolved, and the pH was adjusted to 4.8±0.2 using a pH meter; 8-hydroxyquinoline was dissolved in 10% ethanol solution (volume ratio) and ultrasonically treated (40 kHz, 10 minutes) to form a uniform suspension; gibberellin and 6-benzylpurine were added to 50 mL of anhydrous ethanol and stirred to fully dissolve; sodium thiosulfate and silver nitrate were separately dissolved in 50 mL of distilled water, and then the silver nitrate solution was slowly poured into the sodium thiosulfate while stirring to allow the two solutions to fully dissolve each other, thereby obtaining silver thiosulfate; sodium nitroprusside and ascorbic acid were dissolved in 50 mL of 4°C deionized water in the dark and stored in a brown glass bottle to prevent photolysis failure.

[0039] S3 Gradient Blending:

[0040] To the citric acid solution, add the mixed ethanol solution of GA3 and 6-BA in sequence, and stir with constant temperature magnetic stirring (50°C, 200 rpm) until completely dissolved; slowly add the ethanol suspension of 8-hydroxyquinoline, and stir for 15 minutes in the dark to prevent photolysis; add the mixed solution of sodium nitroprusside and ascorbic acid in the dark at low temperature, and stir thoroughly to mix.

[0041] S4 anti-aging system construction:

[0042] Add freshly prepared STS solution (stock concentration 1 mM, final concentration 80 ppm) in the dark to prevent oxidation and precipitation of silver ions; add vitamin E, vitamin B2, aluminum sulfate and Tween-20, dilute to the target volume with deionized water, and homogenize with a vortex mixer (2000 rpm) for 5 minutes.

[0043] S5 Filtration and Packaging:

[0044] The product was filtered under pressure (pressure 0.3 MPa) through a 0.22 μm nylon filter membrane to remove impurities and undissolved particles; the product was dispensed into brown light-proof glass bottles, sealed with nitrogen, and stored at 4°C.

[0045] Before use, add 60g of sucrose per liter and then process the baby's breath cut flowers.

[0046] Select Gypsophila paniculata "Ilos" with a branch length of about 80 cm and a flower opening degree of about 10%; use sharp scissors to cut the stem base flatly, and put the flower branch into a container with clean water as soon as possible after cutting; thoroughly clean and pre-cool, remove the leaves and side branches and damaged flowers at the bottom third of the flower branch, retain the relatively complete and well-developed flowers and appropriate leaves at the top, trim them neatly according to the length and thickness of the flower stem, and ensure that the inflorescence is upward and arranged uniformly; then simply tie and cut the base of the stem of the Gypsophila paniculata flower branch, cut the bevel in water, and divide it into 3 groups of 3 repetitions, one group with clean water as a blank, and one group (control group) with CN1602689A discloses a Gypsophila paniculata cut flower pretreatment solution, which is composed of 0.8% 8-hydroxyquinoline, 1.2% citric acid, 0.16% 6-benzylpurine, and vitamin E. 0.8%, cis-propylene phosphate 0.1%, and the concentration of polyoxyethylene lauryl ether is 0.3% by volume as a control group, and the other group is treated with this flower-inducing liquid; immediately place them in each test group, with a depth of 5 to 20 cm, and ensure that the lower ends of all stems are immersed in the treatment liquid.

[0047] Place the baby's breath cut flowers in the flower-inducing room, maintain the ambient temperature at 15-30℃, relative humidity at 60%-85%, and light intensity at 1000-3000 lux, avoid direct sunlight and poor ventilation. The flower-inducing time is 3-10 days. Make sure there is about 3L of treatment liquid in the flower-inducing container to ensure that the entire flower-inducing process is continuous and will not be dehydrated.

[0048] Application effect:

[0049] index Treatment group Blank group (clear water) Control group (CN1602689A) Lower bud opening time 5 days 11 days 8 days Upper layer browning rate 10％ 80％ 45％ Product qualification rate 92％ 20％ 65％ Vase life (25℃) 25 days 5 days 14 days

[0050] After being treated with the flower-inducing liquid (control group) of the present invention, the opening rate of the lower layer flower buds was 70% within 5 days, the browning rate of the upper layer was 10%, the vase life was 25 days, and the product qualification rate was 92%. All indicators were higher than those of the blank group (clear water) and the control group (CN1602689A). The phenomenon of withered flowers and buds during the flower-inducing process was avoided, the rapid opening of the lower layer flower buds and the anti-aging synchronization of the upper layer flowers were achieved, and the product qualification rate and vase life were significantly improved.

[0051] Example 2

[0052] The formulation and dosage of a 1L flower-inducing solution include: 10g 8-hydroxyquinoline, 15g citric acid, 80mg gibberellin, 6g ascorbic acid, 60mg silver thiosulfate, 6g aluminum sulfate, 80μmol sodium nitroprusside, 10mg 6-BA, 10g vitamin E, 1g vitamin B2, and 5mL Tween-20. The preparation steps are the same as in Example 1, except that 40g of sucrose per liter is added before use, and then the baby's breath cut flowers are treated.

[0053] Gypsophila paniculata 'Snow Mountain' with a branch length of about 110 cm and a flower opening degree of about 5% was selected, and the processing method was the same as in Example 1.

[0054] Application effect:

[0055]

[0056]

[0057] After being treated with the flower-inducing liquid (control group) of the present invention, the opening rate of the lower flower buds was 65% within 5 days, the browning rate of the upper flower buds was 12%, the vase life was 26 days, and the product qualification rate was 92%. All indicators were higher than those of the blank group (clear water) and the control group (CN1602689A). The phenomenon of withered flowers and buds during the flower-inducing process was avoided, the rapid opening of the lower flower buds and the anti-aging synchronization of the upper flower buds were achieved, and the product qualification rate and vase life were significantly improved.

[0058] Example 3

[0059] The formulation and dosage of a 1L flower-inducing solution include: 6g 8-hydroxyquinoline, 10g citric acid, 120mg gibberellin, 15g ascorbic acid, 100mg silver thiosulfate, 6g aluminum sulfate, 50μmol sodium nitroprusside, 100mg 6-BA, 8g vitamin E, 0.8g vitamin B2, and 5mL Tween-20. The preparation steps are the same as in Example 1, except that 50g of glucose per liter is added before use, and then the baby's breath cut flowers are treated.

[0060] Select Gypsophila paniculata "Starry White" with a branch length of about 100 cm and a flower opening degree of about 10%, and the processing method is the same as Example 1.

[0061] Application effect:

[0062] index Treatment group Blank group (clear water) Control group (CN1602689A) Lower bud opening time 5 days 13 days 10 days Upper layer browning rate 15％ 88％ 40％ Product qualification rate 97％ 18％ 68％ Vase life (25℃) 24 days 4 days 14 days

[0063] After being treated with the flower-inducing liquid (control group) of the present invention, the opening rate of the lower layer flower buds was 65% within 5 days, the browning rate of the upper layer was 15%, the vase life was 24 days, and the product qualification rate was 97%. All indicators were higher than those of the blank group (clear water) and the control group (CN1602689A). The phenomenon of withered flowers and buds during the flower-inducing process was avoided, the rapid opening of the lower layer flower buds and the anti-aging synchronization of the upper layer flowers were achieved, and the product qualification rate and vase life were significantly improved.

[0064] Example 4

[0065] The formulation and dosage of a 1L flower-inducing solution include: 16g 8-hydroxyquinoline, 20g citric acid, 100mg gibberellin, 10g ascorbic acid, 80mg silver thiosulfate, 10g aluminum sulfate, 50μmol sodium nitroprusside, 20mg 6-BA, 6g vitamin E, 0.5g vitamin B2, and 3mL Tween-20. The preparation steps are the same as in Example 1, except that 60g of glucose per liter is added before use, and then the baby's breath cut flowers are treated.

[0066] The baby's breath "Yunxing 03" with a branch length of about 90 cm and a flower opening degree of about 20% was selected, and the processing method was the same as in Example 1.

[0067] Application effect:

[0068] index Treatment group Blank group (clear water) Control group (CN1602689A) Lower bud opening time 4 days 10 days 8 days Upper layer browning rate 17％ 86％ 45％ Product qualification rate 92％ 15％ 60％ Vase life (25℃) 22 days 5 days 14 days

[0069] After treatment with the flower-inducing liquid (control group) of the present invention, the opening rate of the lower layer flower buds was 70% within 5 days, the browning rate of the upper layer was 17%, the vase life was 22 days, and the product qualification rate was 92%. All indicators were higher than those of the blank group (clear water) and the control group (CN1602689A). The phenomenon of withered flowers and buds during the flower-inducing process was avoided, the rapid opening of the lower layer flower buds and the anti-aging synchronization of the upper layer flowers were achieved, and the product qualification rate and vase life were significantly improved.

[0070] Example 5

[0071] The formulation and dosage of a 1L flower-inducing solution include: 6g 8-hydroxyquinoline, 15g citric acid, 80mg gibberellin, 15g ascorbic acid, 80mg silver thiosulfate, 4g aluminum sulfate, 50μmol sodium nitroprusside, 20mg 6-BA, 6g vitamin E, 1g vitamin B2, and 3mL Tween-20. The preparation steps are the same as in Example 1, except that 50g of sucrose per liter is added before use, and then the baby's breath cut flowers are treated.

[0072] The baby's breath "Yunxing 05" with a branch length of about 90 cm and a flower opening degree of about 30% was selected, and the processing method was the same as in Example 1.

[0073] Application effect:

[0074] index Treatment group Blank group (clear water) Control group (CN1602689A) Lower bud opening time 3 days 8 days 6 days Upper layer browning rate 15％ 90％ 40％ Product qualification rate 95％ 15％ 65％ Vase life (25℃) 21 days 7 days 18 days

[0075] After treatment with the flower-inducing liquid (control group) of the present invention, the opening rate of the lower flower buds was 75% within 5 days, the browning rate of the upper flower buds was 15%, the vase life was 21 days, and the product qualification rate was 95%. All indicators were higher than those of the blank group (clear water) and the control group (CN1602689A). The phenomenon of withered flowers and buds during the flower-inducing process was avoided, the rapid opening of the lower flower buds and the anti-aging synchronization of the upper flower buds were achieved, and the product qualification rate and vase life were significantly improved.

[0076] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the preferred embodiment of the present invention is disclosed above, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to equivalent embodiments of equivalent changes using the technical content disclosed above without departing from the scope of the technical solution of the present invention. However, any modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A baby's breath cut flower inducing liquid and a preparation method thereof, characterized in that: The flower-inducing liquid is prepared by dissolving 8-hydroxyquinoline (8-HQ), citric acid, gibberellin, ascorbic acid, silver thiosulfate, aluminum sulfate and Tween-20 in water in proportion.

2. A baby's breath cut flower inducing liquid according to claim 1, characterized in that: The dosage of each component in the flower-inducing liquid is as follows: the concentrations of the following components, measured by weight / volume percentage, are: 0.6%-1.6% of 8-hydroxyquinoline, 1.0%-1.8% of citric acid, 80-150 mg / L of gibberellins, 0.4%-1.2% of ascorbic acid (VC), 60-100 mg / L of silver thiosulfate, and 0.4%-1.5% of aluminum sulfate; and the concentration of Tween-20, measured by volume percentage, is 0.2%-0.5%.

3. A baby's breath cut flower inducing liquid according to claim 1 or 2, characterized in that: The baby's breath cut flower flowering inducing liquid further comprises 50 to 100 μmol / L of sodium nitroprusside (SNP) in volume percentage.

4. A baby's breath cut flower inducing liquid according to claim 1 or 2, characterized in that: The baby's breath cut flower flowering inducing liquid further comprises 10 to 100 mg / L of cytokinin (6-benzylpurine, 6-BA) in mass percentage.

5. A baby's breath cut flower inducing liquid according to claim 1 or 2, characterized in that: The baby's breath cut flower flowering inducing liquid further comprises 0.6% to 1.2% of vitamin E and 0.05% to 0.1% of vitamin B2 in percentage by weight.

6. The flower-inducing liquid for Gypsophila cut flowers according to claims 1 to 5, characterized in that: Specifically, the dosage of each component in the flower-inducing liquid is as follows: the concentrations of the following components, measured by weight / volume percentage, are: 8-hydroxyquinoline 1.0%, citric acid 1.5%, gibberellin 100 mg / L, ascorbic acid 0.8%, silver thiosulfate 80 mg / L, aluminum sulfate 0.8%, sodium nitroprusside 50 μmol / L, 6-BA 30 mg / L, vitamin E 0.6% and vitamin B2 0.05%; and the concentration of Tween-20, measured by volume percentage, is 0.3%.

7. The method for preparing a baby's breath cut flower inducing liquid according to claims 1 to 5, characterized in that: Prepare as follows: S1 raw materials weighed: Accurately weigh each component raw material according to the ratio; S2 raw material pretreatment: Citric acid was dissolved in 50°C deionized water, magnetically stirred (300 rpm) until completely dissolved, and the pH was adjusted to 4.8±0.2 using a pH meter; 8-hydroxyquinoline was dissolved in 10% ethanol solution (volume ratio) and ultrasonically treated (40 kHz, 10 minutes) to form a uniform suspension; gibberellin and 6-benzylpurine were added to 50 mL of anhydrous ethanol and stirred to fully dissolve; sodium thiosulfate and silver nitrate were separately dissolved in 50 mL of distilled water, and then the silver nitrate solution was slowly poured into the sodium thiosulfate while stirring to allow the two solutions to fully dissolve each other, thereby obtaining silver thiosulfate; sodium nitroprusside and ascorbic acid were dissolved in 50 mL of 4°C deionized water in the dark and stored in a brown glass bottle to prevent photolysis failure. S3 Gradient Blending: To the citric acid solution, add the mixed ethanol solution of GA3 and 6-BA in sequence, and stir with constant temperature magnetic stirring (50°C, 200 rpm) until completely dissolved; slowly add the ethanol suspension of 8-hydroxyquinoline, and stir for 15 minutes in the dark to prevent photolysis; add the mixed solution of sodium nitroprusside and ascorbic acid in the dark at low temperature, and stir thoroughly to mix. S4 anti-aging system construction: Add freshly prepared STS solution (stock concentration 1 mM, final concentration 80 mg / L) in the dark to prevent oxidation and precipitation of silver ions; add vitamin E, vitamin B2, aluminum sulfate and Tween-20, dilute to the target volume with deionized water, and homogenize with a vortex mixer (2000 rpm) for 5 minutes. S5 Filtration and Packaging: The product was filtered under pressure (pressure 0.3 MPa) through a 0.22 μm nylon filter membrane to remove impurities and undissolved particles; the product was dispensed into brown light-proof glass bottles, sealed with nitrogen, and stored at 4°C.

8. The method for using the baby's breath cut flower inducing liquid according to claim 1, wherein: The flower-inducing liquid is added with 4% to 8% of sucrose / glucose before use, and then the cut flowers are treated.

9. The method of using the baby's breath cut flower inducing liquid according to claim 1 / 5 / 6, characterized in that: The method comprises the following steps: selecting baby's breath with a branch length of about 70 to 120 centimeters and a flower opening degree of 3% to 35%, cutting the stem base evenly with sharp scissors, and placing the flower branch in a container filled with clean water as soon as possible after cutting; thoroughly cleaning and pre-cooling the flower branch, removing leaves and side branches at the bottom one-third of the flower branch as well as damaged flowers, retaining relatively complete and well-developed flowers and appropriate leaves at the top, trimming the flower stems neatly according to their length and thickness, ensuring that the inflorescences are upward and uniformly arranged; then simply tying and trimming the stem bases of the baby's breath flower branches, cutting an oblique end in water, and immediately placing the branches in a flower-inducing liquid at a depth of 5 to 20 centimeters, ensuring that all lower ends of the stems are immersed in the flower-inducing liquid.

10. The steps of using the baby's breath cut flower inducing liquid according to claim 1 / 5 / 6 / 9, characterized in that: The application of baby's breath cut flower induction liquid in the preservation of baby's breath cut flowers. The ambient temperature of the induction room where the baby's breath flower induction liquid technology is used is maintained at 15-30℃, the relative humidity is 60%-85%, the light intensity is 1000-3000 lux, avoid direct sunlight and poor ventilation, the induction time is 3-10 days, and ensure that there is about 3L of induction liquid in the induction container to ensure that the entire induction process is continuous and will not be dehydrated.

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