Jellyfish discoid body separating and selecting device and using method thereof

Abstract

The invention discloses a jellyfish discoid separation and selection device and a use method thereof, and relates to the technical field of aquatic organism culture and separation devices. Comprising a scyphistoma incubator, a discoid screening pipeline and a circulating filtering device, a water inlet pipe is mounted in the scyphistoma culture box; the disc-shaped body screening pipeline is formed by connecting a plurality of screening units in series, and bolting silk is arranged at the connecting position. The mesh sizes of the plurality of bolting silks are sequentially reduced along the flowing direction; a sealing cover is arranged at the lower part of each screening unit; the circulating filtering device comprises a circulating water tank and a variable-frequency water pump; a water outlet of the scyphistoma incubator is connected with a water inlet of the dish-shaped body screening pipeline, and a water outlet of the dish-shaped body screening pipeline is connected with a water inlet of the circulating filtering device; and the tail end of the water outlet pipe is connected with the water inlet pipe. According to the invention, automatic separation and automatic screening can be realized, manpower and time cost are saved, artificial injury and stress stimulation are reduced, and test accuracy is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of aquatic organism cultivation and separation devices, and in particular to a jellyfish disc-shaped body separation and selection device and a use method thereof. Background Art

[0002] The moon jellyfish, belonging to the phylum Cnidaria, class Scyphozoa, order Cercostomata, family Asteridae, and genus Asterozoa, is a cosmopolitan species widely distributed in coastal waters between 70 degrees north latitude and 40 degrees south latitude. Over the past decade or so, large-scale outbreaks of moon jellyfish have occurred worldwide, severely impacting fisheries, industry, and coastal tourism. Meanwhile, with ornamental jellyfish becoming a new favorite in the aquarium market, moon jellyfish, with their graceful, flowing movements and exceptionally visual appeal, have become a global research hotspot. The life cycle of the moon jellyfish consists of the asexual polyp and sexual medusa stages. Most current research focuses on the polyp stage, where the polyp releases its disc through transverse fission. Therefore, the number of polyp populations determines the number of discs released. However, the survival and growth of discs released during the transverse fission stage also play a crucial role in the size of the medusa population. As early juveniles of the jellyfish's planktonic stage, discs are more sensitive to environmental physicochemical factors and experimental manipulations, and their survival rates during the culture stage are significantly affected by both environmental and human influences.

[0003] To obtain sufficient discs or to study the number of polyps released, current research on discs typically involves incubating corrugated plates containing polyps in specialized jellyfish tanks. By controlling the experimental temperature, the polyps are induced to undergo transverse fission, releasing the discs. During the disc release phase, the discs are removed daily with a pipette and transferred to a temporary disc holding tank. During peak polyp fission activity, thousands of discs can be released daily, resulting in significant labor costs associated with the separation and selection process. Furthermore, the newly released discs are small, with some even clinging to the corrugated plates. Furthermore, the transparent nature and small diameter of newly released discs significantly hinder transfer, making it difficult for researchers to transfer all of them. Unsuccessful transfers can lead to loss during subsequent feeding and water changes, thus affecting statistical accuracy. Furthermore, the process of transferring the discs with a pipette causes mechanical damage and stress to the discs. On the other hand, the disc-shaped bodies transferred to the disc-shaped body incubator have different body lengths. In the subsequent grouping experiments, they need to be grouped according to the different umbrella diameters. Disc-shaped bodies of different umbrella diameters are mixed together, and the selection process will consume a lot of manpower and time costs.

[0004] Therefore, there is an urgent need to develop a novel disc-shaped separation and screening device. Summary of the Invention

[0005] The main purpose of the present invention is to provide a jellyfish disc-shaped body separation and sorting device and its use method to solve the above problems.

[0006] To achieve the above-mentioned purpose, the present invention provides a jellyfish disc-shaped body separation and selection device, comprising a polyp incubator, a disc-shaped body screening pipeline and a circulation filtering device; a water inlet pipe is installed in the polyp incubator, and a water outlet is provided at the lower part of the side wall of the polyp incubator; the disc-shaped body screening pipeline is composed of a plurality of screening units connected in series, and a screen is provided at the connection between two adjacent screening units; the mesh size of the plurality of screens decreases successively along the flow direction; a sealing cover is provided at the lower part of each screening unit; the circulation filtering device comprises a circulating water tank and a variable frequency water pump arranged in the circulating water tank, and a water inlet is provided at the lower part of the side wall of the circulating water tank; the water outlet of the polyp incubator is connected to the water inlet of the disc-shaped body screening pipeline, and the water outlet of the disc-shaped body screening pipeline is connected to the water inlet of the circulation filtering device; the water outlet of the variable frequency water pump is connected to the outlet pipe, and the end of the outlet pipe is connected to the water inlet pipe of the polyp incubator.

[0007] Furthermore, the polyp incubator is cylindrical.

[0008] Furthermore, the water inlet pipe is cylindrical and vertically arranged, the top of the water inlet pipe is located outside the polyp incubator and connected to the water outlet pipe, the lower end of the water inlet pipe is a closed end and connected to the bottom plate of the polyp incubator, and there are multiple circular holes arranged in a straight line up and down on the side wall of the water inlet pipe.

[0009] Furthermore, the screening unit is cylindrical, with sealing flanges installed at both ends, and two adjacent screening units are connected via the sealing flanges.

[0010] Furthermore, filter material is placed in the circulating water tank.

[0011] Furthermore, a temperature control device is provided in the circulating water tank.

[0012] The present invention also provides a method for using a jellyfish disc-shaped body separation and selection device, comprising the following steps:

[0013] S1. Trim the corrugated plate containing jellyfish polyps in the laboratory to a suitable size, record the number of polyps on the plate, and then place it in a polyp incubator.

[0014] S2. Open the circulating filter device to adapt the water temperature. During the adaptation phase, the water temperature is controlled at around 18°C.

[0015] S3. The experiment set up five temperature change groups. After the polyps adapted for one week, the water temperature was gradually adjusted, decreasing or increasing at a rate of 1°C per day. After reaching the preset experimental temperature, the polyp transverse splitting test was carried out.

[0016] S4. During the early stages of polyp fission, closely monitor the release of discs from the polyp incubator. Once the polyps enter the fission stage, adjust the water flow rate to ensure fluidity without affecting the individual motion of the discs.

[0017] S5. Each temperature change group has 5 umbrella diameter ranges. Therefore, the disc separation pipeline has a total of 5 screening units;

[0018] S6. During the polyp transverse fission stage, at a fixed time each day, suspend the circulating filtration device, open the sealing covers at the bottom of the five screening units, transfer the water to the corresponding incubator, then disassemble the screening units, rinse the screening units and the corresponding sieves to separate all remaining unseparated discs, and record the number of discs of the five sizes in the five temperature change groups;

[0019] S7. Install the screening unit and repeat this step every day during the polyp transverse fission stage, recording the number of discs placed;

[0020] S8. Discoids of different sizes and release times were cultured separately to continue the early discoid experiment. Five body length groups were set up in the experiment. At this time, only healthy individuals need to be randomly selected from the incubators with different body lengths. There is no need to measure the umbrella diameter, which makes the experiment more convenient and accurate.

[0021] The present invention has the following beneficial effects:

[0022] The present invention can automatically separate polyps and disc-shaped bodies, and automatically screen them according to the size of the umbrella diameter, integrating the separation and screening functions in one, saving manpower and time costs. When separating the disc-shaped bodies, it is only necessary to open the sealing cover and directly transfer the water containing the disc-shaped bodies. Compared with the experimenter using a straw to suck, the invention reduces human damage and stress stimulation, and improves the survival rate of the disc-shaped bodies and the accuracy of indoor experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The figure is an overall schematic diagram of a jellyfish disc-shaped body separation and selection device according to the present invention.

[0024] Figure 2 This is a schematic diagram of a screening unit of a jellyfish disc-shaped body separation and selection device according to the present invention.

[0025] Among them, 1-polyp culture box; 2-screening unit; 3-sieve silk; 4-circulating filtration device; 11-water inlet pipe; 12-circular small hole; 13-corrugated plate; 21-sealing flange; 22-sealing cover; 41-circulating water tank; 42-variable frequency water pump; 43-water outlet pipe. DETAILED DESCRIPTION

[0026] In order to achieve the above-mentioned objectives and effects, the technical means and structures adopted by the present invention are described in detail with reference to the accompanying drawings for the features and functions of the preferred embodiments of the present invention.

[0027] like Figure 1-2As shown, the present invention provides a jellyfish disc separation and selection device, including a polyp incubator 1, a disc screening pipe and a circulating filtration device 4; the polyp incubator 1 is cylindrical, which reduces the formation of corners in the box and increases the smoothness of the disc swimming, effectively solving the problem that the existing square culture bottle will produce bottom corners and the disc is easily stuck in the corner, resulting in incomplete separation of the disc; a water inlet pipe 11 is installed in the polyp incubator 1, the water inlet pipe 11 is cylindrical and vertically arranged, the top end of the water inlet pipe 11 is located outside the polyp incubator 1 and is connected to the water outlet pipe 43, the lower end of the water inlet pipe 11 is a closed end and is connected to the bottom plate of the polyp incubator 1, and the water inlet pipe There are multiple circular small holes 12 arranged in a straight line up and down on the side wall of 11; the linearly arranged porous structure can effectively generate moving water flow at different levels inside the incubator to prevent the released disc-shaped bodies from adhering to the corrugated plate. Since the newly released disc-shaped bodies have weak mobility, a single water inlet may cause the disc-shaped bodies to gather in a corner of the incubator and fail to be successfully separated. The design of the multi-porous water inlet can effectively increase the fluidity of the water in the incubator and increase the number of disc-shaped bodies separated; a water outlet is provided at the lower part of the side wall of the polyp incubator 1; the disc-shaped body screening pipeline is composed of multiple screening units 2 in series, and the screening unit 2 is cylindrical with sealing flanges 21 installed at both ends. There is a seal between two adjacent screening units 2. The filter elements 2 are connected by sealing flanges 21; a screen silk 3 is provided at the connection between two adjacent screening units 2; the mesh size of the screen silk 3 can be set according to the umbrella diameter size of the target disc-shaped body; the mesh sizes of the multiple screen silks 3 are successively reduced along the flow direction so that disc-shaped bodies with different umbrella diameter sizes can be obtained in each screening unit; a sealing cover 22 is provided at the bottom of each screening unit 2, and the disc-shaped bodies obtained therein can be separated after the sealing cover 22 is opened; when a certain number of disc-shaped bodies are obtained, the circulating filtration device 4 is suspended. Since the newly released disc-shaped bodies have weak movement ability, the disc-shaped body backflow phenomenon will not occur. Therefore, there is no need to disassemble the screening unit 2, and the sealing cover 22 at the bottom of each screening unit 2 is directly opened to transfer the water inside Move it to the corresponding incubator, then disassemble each screening unit 2, gently rinse the screening unit 2 and the corresponding sieve silk 3 with water, and completely separate the remaining disc-shaped bodies attached to the sieve silk 3 and the screening unit 2; the circulating filtration device 4 includes a circulating water tank 41 and a variable frequency water pump 42 arranged in the circulating water tank 41, and a water inlet is provided at the lower part of the side wall of the circulating water tank 41; the water outlet of the polyp incubator 1 is connected to the water inlet of the disc-shaped body screening pipe, and the water outlet of the disc-shaped body screening pipe is connected to the water inlet of the circulating filtration device 4; the water outlet of the variable frequency water pump 42 is connected to the outlet pipe 43, and the end of the outlet pipe 43 is connected to the water inlet pipe 11 of the polyp incubator 1.By adjusting the flow rate of the variable frequency water pump 42, the water flow rate in the entire separation and selection device can be controlled to prevent damage to the polyps and discs caused by excessive flow; filter materials such as coral stones and ceramic rings can be placed in the circulating water tank 41 to ensure water quality. At the same time, water body physical and chemical factor controllers such as temperature control devices and dissolved oxygen control devices can be installed to adjust the water body physical and chemical factors externally, thereby achieving precise control of the water body physical and chemical factors without affecting the polyps and discs.

[0028] The present invention also provides a method for using a jellyfish disc-shaped body separation and selection device, comprising the following steps:

[0029] S1. Trim the corrugated plate 13 with attached polyps of jellyfish cultured in the laboratory to a suitable size, record the number of polyps on the corrugated plate 13, and then place it in a polyp incubator;

[0030] S2, open the circulation filter device 4, and adapt the water temperature. During the adaptation stage, the water temperature is controlled at about 18°C;

[0031] S3. The experiment set up five temperature change groups. After the polyps adapted for one week, the water temperature was gradually adjusted, decreasing or increasing at a rate of 1°C per day. After reaching the preset experimental temperature, the polyp transverse splitting test was carried out.

[0032] S4. In the early stage of polyp fission, closely monitor whether the disc-shaped bodies are released from the polyp incubator 1. When the polyps enter the fission stage, adjust the water flow rate to achieve the fluidity of the water body without affecting the movement posture of the individual disc-shaped bodies.

[0033] S5. Each temperature change group has 5 umbrella diameter ranges. Therefore, the disc-shaped separation pipeline has a total of 5 screening units 2;

[0034] S6. During the polyp transverse fission stage, at a fixed time each day, suspend the circulating filtration device 4, open the sealing covers 22 at the bottom of the five screening units, transfer the water to the corresponding incubator, then remove the screening unit 2, rinse the screening unit 2 and the corresponding sieve 3 to separate all remaining unseparated discs, and record the number of discs of the five sizes in the five temperature change groups;

[0035] S7, install the screening unit 2, repeat this step every day during the polyp transverse fission stage, and record the number of discs placed;

[0036] S8. Discoids of different sizes and release times were cultured separately to continue the early discoid experiment. Five body length groups were set up in the experiment. At this time, only healthy individuals need to be randomly selected from the incubators with different body lengths. There is no need to measure the umbrella diameter, which makes the experiment more convenient and accurate.

[0037] This separation and selection device can achieve precise control of the physical and chemical factors of the water body and achieve consistency of the water body indicators of the entire separation device. In particular, for relatively sensitive physical and chemical factors of the water body such as dissolved oxygen, it is only necessary to adjust the water body in the circulating water tank 41 to the required standard. The entire separation and selection device forms an internal loop, which can ensure precise control of the physical and chemical factors. Moreover, the circulating filtration device 4 exists independently outside the polyp culture box 1 and the disc separation pipeline, and the physical and chemical factor regulation is more convenient. The adjustment process will not affect the polyps and discs in the device. When separating the discs, it is only necessary to open the sealing cover 22 and directly transfer the water body containing the discs. Compared with the experimenter using a straw to suck, it reduces human damage and stress stimulation, reduces labor costs, and increases the accuracy of indoor experiments.

[0038] In addition to separating and selecting the disc-shaped bodies of moon jellyfish, the present invention can also test the disc-shaped bodies of different types of jellyfish. In addition, the size of the screening unit 2 can be adjusted according to the size of different test organisms, so that it can be used for more species.

[0039] The above descriptions are only preferred embodiments of the present invention, not all embodiments. Anyone should be aware that any structural changes made under the guidance of the present invention, and any technical solutions that are the same or similar to those of the present invention, fall within the scope of protection of the present invention.

Claims

1. A jellyfish disc separation and sorting device, characterized in that: It includes a polyp incubator, a disc-shaped body screening pipe and a circulation filtering device; a water inlet pipe is installed in the polyp incubator, and a water outlet is provided at the lower part of the side wall of the polyp incubator; the disc-shaped body screening pipe is composed of a plurality of screening units connected in series, and a screen silk is provided at the connection between two adjacent screening units; the mesh sizes of the plurality of screen silks decrease successively along the flow direction; a sealing cover is provided at the lower part of each screening unit; the circulation filtering device includes a circulating water tank and a variable frequency water pump arranged in the circulating water tank, and a water inlet is provided at the lower part of the side wall of the circulating water tank; the water outlet of the polyp incubator is connected to the water inlet of the disc-shaped body screening pipe, and the water outlet of the disc-shaped body screening pipe is connected to the water inlet of the circulation filtering device; the water outlet of the variable frequency water pump is connected to the outlet pipe, and the end of the outlet pipe is connected to the water inlet pipe of the polyp incubator.

2. The jellyfish disc separation and sorting device according to claim 1, characterized in that: The polyp culture box is cylindrical.

3. The jellyfish disc separation and sorting device according to claim 1, characterized in that: The water inlet pipe is cylindrical and vertically arranged. The top end of the water inlet pipe is located outside the polyp incubator and is connected to the water outlet pipe. The lower end of the water inlet pipe is a closed end and is connected to the bottom plate of the polyp incubator. There are multiple circular holes arranged in a straight line up and down on the side wall of the water inlet pipe.

4. The jellyfish disc separation and sorting device according to claim 1, characterized in that: The screening unit is cylindrical, with sealing flanges installed at both ends, and two adjacent screening units are connected via the sealing flanges.

5. The jellyfish disc separation and sorting device according to claim 1, characterized in that: Filter material is placed in the circulating water tank.

6. The jellyfish disc separation and sorting device according to claim 1, characterized in that: A temperature control device is provided in the circulating water tank.

7. A method for using a jellyfish disc separation and sorting device, characterized in that: The jellyfish disc separation and sorting device according to any one of claims 1 to 6 comprises the following steps: S1. Trim the corrugated plate containing jellyfish polyps in the laboratory to a suitable size, record the number of polyps on the plate, and then place it in a polyp incubator. S2. Open the circulating filter device to adapt the water temperature. During the adaptation phase, the water temperature is controlled at around 18°C. S3. The experiment set up five temperature change groups. After the polyps adapted for one week, the water temperature was gradually adjusted, decreasing or increasing at a rate of 1°C per day. After reaching the preset experimental temperature, the polyp transverse splitting test was carried out. S4. During the early stages of polyp fission, closely monitor the release of discs from the polyp incubator. Once the polyps enter the fission stage, adjust the water flow rate to ensure fluidity without affecting the individual motion of the discs. S5. Each temperature change group has 5 umbrella diameter ranges. Therefore, the disc separation pipeline has a total of 5 screening units; S6. During the polyp transverse fission stage, at a fixed time each day, suspend the circulating filtration device, open the sealing covers at the bottom of the five screening units, transfer the water to the corresponding incubator, then disassemble the screening units, rinse the screening units and the corresponding sieves to separate all remaining unseparated discs, and record the number of discs of the five sizes in the five temperature change groups; S7. Install the screening unit and repeat this step every day during the polyp transverse fission stage, recording the number of discs placed; S8. Discoids of different sizes and release times were cultured separately to continue the early discoid experiment. Five body length groups were set up in the experiment. At this time, only healthy individuals need to be randomly selected from the incubators with different body lengths. There is no need to measure the umbrella diameter, which makes the experiment more convenient and accurate.

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