Beverage with effects of soothing nerves, helping sleep and resisting depression and preparation method thereof

Abstract

The invention belongs to the technical field of nerve soothing beverages, and particularly relates to a beverage with nerve soothing, sleep aiding and depression resisting effects and a preparation method thereof.The beverage is formed by compounding a fingered citron extracting solution and multiple medicinal and edible plant auxiliary materials and comprises light wheat, valerian, polygala tenuifolia, tuber fleeceflower stem, lily, cortex albiziae, lotus plumule and other ingredients with the nerve regulation effect. The composite liquid preparation is prepared through combined extraction of ethanol and water and auxiliary preparation of a stabilizer. Chronic stress modeling mouse experiments prove that the beverage significantly improves depression behavior indexes, improves sucrose preference, shortens standing time, can effectively prolong total sleep time, and has good emotion regulation and neuroprotection effects. Compared with comparative drinks lacking synergistic compatibility components, the embodiment group is superior in the aspects of depression resistance and sleep aiding, the scientificity and function reliability of the formula are proved, and the beverage is suitable for the field of development of health drinks or functional drinks.

Description

Technical Field

[0001] The present invention belongs to the technical field of tranquilizing drinks, and in particular relates to a drink with tranquilizing, sleep-inducing and anti-depressant effects and a preparation method thereof. Background Art

[0002] With the accelerating pace of modern life, emotional issues such as increased stress, sleep disorders, anxiety, and depression are becoming increasingly prominent and have become significant factors affecting people's physical and mental health. Long-term mood swings and insomnia not only weaken the body's immune system but may also induce chronic conditions such as cardiovascular and cerebrovascular diseases and endocrine disorders. Therefore, the development of natural beverages with calming, sleep-inducing, and antidepressant properties has become a key research area in the functional food field.

[0003] Currently, sleep-aid or antidepressant products on the market primarily consist of chemical drugs and some Chinese herbal preparations. While these can alleviate symptoms in the short term, long-term use can lead to dependency, side effects, and liver and kidney stress, making them unsuitable for regular daily health drinks. In contrast, functional beverages developed from natural plant resources, particularly those using traditional Chinese medicinal herbs with a history of medicinal and edible uses, have attracted significant attention due to their safety, mild conditioning effects, and long-term drinking potential.

[0004] Buddha's hand (Citrus medica L. var. sarcodactylis), a plant of the Rutaceae family, has multiple pharmacological effects, including regulating qi and resolving phlegm, soothing the stomach and relieving pain, and relieving liver depression. Modern pharmacological research has shown that Buddha's hand contains a variety of volatile oils, flavonoids, coumarins, and polysaccharides, which have biological activities such as regulating the central nervous system, alleviating anxiety and depression, and improving sleep quality.

[0005] However, the existing technology still lacks a complex beverage product that uses bergamot as the main raw material, is combined with a variety of tranquilizing plant ingredients, and has good taste, stability and functionality. Its preparation process, ingredient ratio and function verification methods still need further systematic research and standardization. Summary of the Invention

[0006] In response to the above problems, the purpose of the present invention is to propose: a beverage with calming, sleep-inducing and anti-depressant effects, comprising the following components by weight: 60-120 parts of bergamot extract, 150-200 parts of auxiliary material extract, 2-3 parts of stabilizer, 300-400 parts of water, 5-6 parts of sucrose, and 3-4 parts of edible flavoring.

[0007] In a preferred technical solution, the bergamot extract is a mixed extract obtained by extracting the following plant raw materials in parts by weight: 80-100 parts of bergamot arborvitae, 80-100 parts of immature bitter orange, 60-80 parts of floating wheat, 60-80 parts of valerian, 80-100 parts of ganoderma lucidum, 60-80 parts of polygala tenuifolia, 60-80 parts of tuberose vine, 60-80 parts of lily, 60-80 parts of julibrissin bark, and 30-50 parts of lotus seed core.

[0008] In a preferred technical solution, the auxiliary material extract is a mixed extract obtained by extracting the following plant raw materials in parts by weight: 30-40 parts of jasmine tea, 4-6 parts of perilla leaves, 5-7 parts of pomegranate flowers, 6-8 parts of lilies, 4-6 parts of Panax notoginseng, 4-5 parts of licorice, 5-6 parts of hawthorn, 8-10 parts of scutellaria, 6-8 parts of julibrissin bark, and 3-5 parts of lotus seed core.

[0009] In a preferred technical solution, the stabilizer is a mixture of carrageenan and ammonium polyacrylate, and the mixing ratio of carrageenan to ammonium polyacrylate is (0.5-0.8):1.

[0010] In a preferred technical solution, the edible flavor is selected from one of bergamot flavor, citrus flavor, jasmine flavor, mint flavor or a compound flavor thereof.

[0011] The present invention also provides a method for preparing the beverage having the effects of calming the mind, promoting sleep and combating depression, comprising the following steps: S1, preparing a bergamot extract; taking a predetermined amount of bergamot extract raw material, crushing, extracting, drying, and dissolving to obtain a bergamot extract; S2. Preparation of auxiliary material extract: After the auxiliary material extract raw materials are grouped, low-temperature extraction and medium-temperature extraction are performed respectively to obtain auxiliary material extract; S3, compounding the bergamot extract, auxiliary material extract, water, sucrose, and edible flavor according to a predetermined ratio to obtain a basic compound solution; S4, stabilization treatment: adding 2-3 parts of a stabilizer to the base compound solution, wherein the stabilizer is a mixture of carrageenan and ammonium polyacrylate in a mixing ratio of (0.5-0.8):1, and stirring to obtain a mixed solution; S5, sterilization: pasteurizing or ultra-high temperature instantaneous sterilization of the mixed solution; S6. Cooling and filling: The sterilized beverage is cooled to room temperature and filled to obtain the finished product.

[0012] In a preferred technical solution, step S1 specifically includes: S11. Ingredients: 80-100 parts of Citrus aurantium immaturus, 80-100 parts of Aralia citriodora, 60-80 parts of Triticum tiliaceum, 60-80 parts of Valerian root, 80-100 parts of Ganoderma lucidum, 60-80 parts of Polygala tenuifolia, 60-80 parts of Polygonum multiflorum, 60-80 parts of Lilium bulbs, 60-80 parts of Albizia julibrissin bark, and 30-50 parts of Lotus seed core, by weight; S12, grinding: washing and air-drying the raw materials in sequence, grinding them after air-drying, and passing them through a 120-mesh sieve to obtain raw material powder; S13, ethanol extraction: Place the raw material powder in an extraction tank, add 8-10 times the total weight of ethanol solution, with a concentration of 80%-90%, seal and extract for 48-62 hours. After the extraction is completed, let it stand and filter to separate the residue layer and the liquid layer; S14, water extraction: add 8-10 times the total weight of deionized water to the extraction tank, seal and extract for 48-62 hours, then let it stand and filter to obtain the second liquid layer; S15, concentration and drying: The liquid layers obtained in steps S3 and S4 are mixed, allowed to stand and precipitated twice, and the supernatant is collected, concentrated, and spray-dried to obtain a powdered extract; S16, dissolving: weighing 60-120 parts of the powdered extract, adding it to 600-700 parts of purified water to dissolve it, and stirring it evenly; In a preferred technical solution, step S2 specifically includes: S21. Grouping and taking auxiliary materials: Divide the auxiliary materials into two groups: The first group includes: 30–40 parts of jasmine tea, 4–6 parts of perilla leaves, 5–7 parts of pomegranate flowers, and 6–8 parts of lily; the second group includes: 4–6 parts of Panax notoginseng, 4–5 parts of liquorice, 5–6 parts of hawthorn, 8–10 parts of scutellaria, 6–8 parts of julibrissin bark, and 3–5 parts of lotus seed core; S22, first group extraction process: The first group of auxiliary materials were washed and air-dried in sequence, and then ground into powder until they could pass through a 120-mesh sieve; Take the first group of powders and add them into deionized water with a volume of 8–10 times the total weight, and extract them by slow stirring at 50–60°C for 30–40 minutes; After completion, coarse filter and let it settle, take the supernatant and mark it as the first auxiliary material extract for later use; S23, second group extraction process: The second group of auxiliary materials were washed, air-dried and then pulverized to pass through a 120-mesh sieve; Add 8–10 times the weight of deionized water and extract at 70–80°C for 1.5–2 hours with intermittent stirring; After completion, coarse filter and let stand to settle, take the supernatant and mark it as the second auxiliary material extract; S24, auxiliary liquid merging: The first auxiliary material extract and the second auxiliary material extract were combined in a ratio of 1:1, filtered to remove impurities, and then stirred to obtain an auxiliary material extract.

[0013] In a preferred technical solution, step S3 specifically includes: S31, compounding treatment: placing 60-120 parts of the bergamot extract and 150-200 parts of the auxiliary material extract into a stirring tank, turning on the stirring device, and premixing at a low speed of 50-100 rpm for 1-2 minutes to preliminarily mix the bergamot extract and the auxiliary material extract to form a stable liquid phase base; S32, adding 300-400 parts of the predetermined purified water to the stable liquid phase base, and continuing to stir until the system is uniform and transparent; S33. While stirring, add 5-6 parts of sucrose and 3-4 parts of edible flavoring in sequence, and continue stirring until all ingredients are fully dissolved and mixed to form a basic compound liquid.

[0014] Beneficial effects 1. The present invention uses bergamot extract as the core of its formula design, combining it with a variety of medicinal and edible plants with calming, sleep-inducing, and mood-regulating functions to achieve synergistic enhancement effects through scientific compatibility. Bergamot and Polygala tenuifolia synergistically enhance brain nerve regulation and mood stabilization; together with Polygonum multiflorum, it exerts a calming, sleep-inducing, and anxiety-relieving effect; combined with Albizia julibrissin bark, it helps relieve depression and improve sleep structure; combined with lily bulb, it moistens the lungs, nourishes yin, tonifies the mind, and improves symptoms such as restlessness and waking easily; and combined with lotus seed core, it further balances the dual needs of clearing the heart and reducing internal heat while calming the mind. 2. The main ingredients are combined with auxiliary ingredients such as jasmine tea, licorice, pomegranate flowers, and perilla leaves to harmonize the overall flavor, enhance the pleasant taste, and play a role in sustained release of medicinal properties and improving physical sensation. The above combination shows significant advantages in dosage form stability, efficacy coordination, and synergy of plant compounds, constructing a complex functional beverage that combines plant efficacy and sensory experience.

[0015] 3. This invention innovatively adopts an excipient grouping extraction process. According to the volatility and heat sensitivity of different excipients, low-temperature short-time extraction and medium-temperature long-time extraction processes are designed respectively, so as to retain active ingredients such as aromatics, flavonoids, and polyphenols to the greatest extent, avoid thermal degradation of effective substances and aroma loss, and improve extraction efficiency and ingredient integrity. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 Schematic diagram of the method flow of the present invention (taking Example 1 as an example); Figure 2 Schematic diagram of the comparative experiment of the present invention (behavioral experiment-immobility time); Figure 3 Schematic diagram of the comparative experiment of the present invention (behavioral experiment - sucrose preference); Figure 4 Schematic diagram of the comparative experiment of the present invention (sleep experiment-sleep latency); Figure 5 Schematic diagram of the comparative experiment of the present invention (sleep experiment-total sleep time). DETAILED DESCRIPTION

[0017] In order to deepen the understanding of the present invention, the present invention will be further described in detail below with reference to the examples. The examples are only used to explain the present invention and do not constitute a limitation on the scope of protection of the present invention.

[0018] Example 1 (T1) This embodiment provides a beverage with tranquilizing, sleep-inducing and anti-depressant effects, which comprises the following components by weight: 90 parts of bergamot extract, 180 parts of auxiliary material extract, 2.5 parts of stabilizer, 350 parts of purified water, 5.5 parts of sucrose, and 3.5 parts of edible flavoring.

[0019] The bergamot extract is obtained by extracting the following plant raw materials in the following weight ratios: 90 parts of arborvitae, 90 parts of immature bitter oranges, 70 parts of wheat, 70 parts of valerian, 90 parts of ganoderma lucidum, 70 parts of polygala, 70 parts of tuberculosis, 70 parts of lily, 70 parts of julibrissin bark, 40 parts of lotus seed core.

[0020] Its preparation method is as follows Figure 1 Shown, including: S1. Preparation of bergamot extract: S11. Materials: Weigh 760 parts of raw materials, including bergamot, immature bitter orange, floating wheat, valerian, ganoderma lucidum, polygala tenuifolia, celery, lily, julibrissin bark, and lotus seed core, according to the above proportions; S12, clean, air-dry and then grind to 120 mesh; S13, ethanol extraction: add 80% ethanol solution (with a mass ratio of 1:10 to the total medicinal material), seal and extract for 48 hours, and filter to obtain the first extract; S14, water extraction: deionized water (ratio 1:10) was added, and the mixture was extracted at 80°C for 1.5 hours, and filtered to obtain the second extract; S15, mixing the two extracts, settling twice, collecting the supernatant, concentrating under reduced pressure to 1 / 5 of the original volume, and spray drying to obtain an extract powder; S16, re-dissolving: weigh 90 parts of the above extract powder, add 600 parts of purified water, stir and dissolve, and obtain the bergamot extract.

[0021] S2. Preparation of auxiliary material extract: S21. Raw materials are grouped as follows: Group 1: 35 parts of jasmine tea, 5 parts of perilla leaves, 6 parts of pomegranate flowers, and 7 parts of lily; Group 2: 5 parts of Panax notoginseng, 4.5 parts of Licorice, 5.5 parts of Crataegus, 9 parts of Scutellaria, 7 parts of Albizia Julibrissin, 4 parts of Lotus Seed Heart; S22, the first group of auxiliary materials were washed, air-dried, and then crushed to 120 mesh size, and 8 times purified water was added. The temperature was controlled at 55°C, and the mixture was stirred and extracted for 40 minutes. The precipitate was filtered to obtain the first extract; S23, the second set of auxiliary materials were washed, air-dried, and then crushed to 120 mesh size, and 10 times of deionized water was added. The temperature was controlled at 75°C, and the extraction was carried out for 90 minutes. The precipitate was filtered to obtain the second extract; S24. Combine the two groups of extracts (volume ratio 1:1), filter thoroughly to remove impurities, and obtain 180 parts of auxiliary material extract.

[0022] S3. Compounding: S31, pouring 90 parts of the bergamot extract and 180 parts of the auxiliary material extract into a mixing tank, stirring at a low speed (70 rpm) for 1.5 minutes to form a preliminary mixed solution; S32, add 350 parts of purified water, and continue stirring until the mixture is uniform and transparent; S33, add 5.5 parts of sucrose and 3.5 parts of edible flavoring (preferably a mixture of bergamot flavoring and jasmine flavoring), and continue stirring until all are dissolved to obtain a basic compound liquid.

[0023] S4, stabilization processing: 2.5 parts of stabilizer were added, wherein the stabilizer was carrageenan and ammonium polyacrylate premixed in a ratio of 0.7:1 and then used, and stirred for 10 minutes to form a stable liquid phase system.

[0024] S5. Sterilization: Ultra-high temperature instantaneous sterilization (UHT) is used, with a temperature control of 92°C and a sterilization time of 12 seconds to ensure microbial safety.

[0025] S6, cooling and filling: After sterilization, the beverage is naturally cooled to room temperature, and aseptically filled and sealed to obtain the finished beverage.

[0026] Example 2 (T2) This embodiment provides a drink with calming, sleep-inducing and anti-depressant effects, which includes the following components by weight: 60 parts of bergamot extract, 150 parts of auxiliary material extract, 2 parts of stabilizer, 300 parts of purified water, 5 parts of sucrose, and 3 parts of edible flavoring.

[0027] The bergamot extract is obtained by extracting the following plant raw materials in parts by weight: 80 parts of bergamot arborvitae, 80 parts of immature bitter orange, 60 parts of floating wheat, 60 parts of valerian, 80 parts of ganoderma lucidum, 60 parts of polygala tenuifolia, 60 parts of tuberose vine, 60 parts of lily, 60 parts of julibrissin bark, and 30 parts of lotus seed core.

[0028] The preparation method is as follows: S1. Preparation of bergamot extract: S11, taking materials: weighing a total of 630 parts of raw materials according to the above ratio; S12, washing, air-drying and then crushing to 120 mesh; S13, ethanol extraction: add 80% ethanol solution (ratio to raw material is 1:10), seal and extract for 48 hours, let it stand and filter to obtain the first liquid; S14, water extraction: similarly add 10 times the amount of deionized water, extract at 80°C for 1.5 hours, and filter to obtain the second liquid; S15, combining the two extracts, allowing them to settle twice, collecting the supernatant, concentrating it to 1 / 5 of its original volume, and spray drying it to obtain an extract powder; S16, re-dissolving: weigh 60 parts of the extract powder, add it into 600 parts of purified water, stir and dissolve it thoroughly, and obtain the bergamot extract.

[0029] S2. Preparation of auxiliary material extract: S21. Excipients are grouped as follows: Group 1: 30 parts of jasmine tea, 4 parts of perilla leaves, 5 parts of pomegranate flowers, and 6 parts of lily; Group 2: 4 parts of Panax notoginseng, 4 parts of Licorice, 5 parts of Crataegus, 8 parts of Scutellaria, 6 parts of Albizia Julibrissin, 3 parts of Lotus Seed Heart; S22, the first group of auxiliary materials were washed, air-dried, and then crushed to 120 mesh size, and 8 times the amount of water was added. The mixture was extracted at 55°C for 30 minutes, and the precipitate was filtered to obtain the first extract; S23, the second group of auxiliary materials were washed, air-dried, and then crushed to 120 mesh size, and 10 times the amount of water was added. The mixture was extracted at 70°C for 1.5 hours, and filtered to obtain the second extract; S24. Combine the two liquids in a 1:1 ratio, filter and remove impurities to obtain 150 parts of auxiliary material extract.

[0030] S3, compounding steps: S31, placing 60 parts of the bergamot extract and 150 parts of the auxiliary material extract in a stirring tank, starting the stirring device, adjusting the speed to 50 rpm, and premixing at a low speed for 1 minute; S32, add 300 parts of purified water, and continue stirring until the mixture is uniform and clear; S33, add 5 parts of sucrose and 3 parts of edible flavoring (preferably a mixture of citrus flavoring and jasmine flavoring), and continue stirring until fully dissolved to form a basic compound liquid.

[0031] S4, stabilization processing: Add 2 parts of stabilizer, wherein carrageenan and ammonium polyacrylate are mixed in a ratio of 0.5:1, and stir and homogenize for 8 minutes to form a uniform system.

[0032] S5. Sterilization treatment: The composite beverage is pasteurized at a temperature of 85° C. for 30 minutes.

[0033] S6. Cooling and filling: After sterilization, the beverage is naturally cooled to room temperature, filled into sterile bottles and sealed to obtain a stable finished beverage.

[0034] Example 3 (T3) This embodiment provides a drink with calming, sleep-inducing and anti-depressant effects, which includes the following components by weight: 120 parts of bergamot extract, 200 parts of auxiliary material extract, 3 parts of stabilizer, 400 parts of purified water, 6 parts of sucrose, and 4 parts of edible flavoring.

[0035] Among them, the bergamot extract is obtained by extracting the following plant raw materials in the following weight ratio: 100 parts of bergamot arborvitae, 100 parts of immature bitter orange, 80 parts of floating wheat, 80 parts of valerian, 100 parts of ganoderma lucidum, 80 parts of polygala tenuifolia, 80 parts of tuberose vine, 80 parts of lily, 80 parts of julibrissin bark, and 50 parts of lotus seed core.

[0036] The preparation method is as follows: S1. Preparation of bergamot extract: S11, taking materials: weighing a total of 830 parts of raw materials according to the above ratio; S12, washing and air-drying in sequence, and crushing to 120 mesh after air-drying; S13, ethanol extraction: Pour the powder into an extraction tank, add 80% ethanol (1:10 mass ratio of raw material), seal and extract for 62 hours, and filter after extraction to obtain the first extract; S14, water extraction: add an equal amount of deionized water, extract at 90°C for 2 hours, and filter to obtain the second extract; S15, mixing the two extracts, allowing them to settle twice, concentrating the supernatant to 1 / 5 of the original volume, and spray-drying to obtain a powder extract; S16, re-dissolving: weigh 120 parts of the powder, add 700 parts of purified water and stir evenly to obtain a bergamot extract.

[0037] S2. Preparation of auxiliary material extract: S21. Excipients are grouped as follows: Group 1: 40 parts of jasmine tea, 6 parts of perilla leaves, 7 parts of pomegranate flowers, and 8 parts of lily; Group 2: 6 parts of Panax notoginseng, 5 parts of Licorice, 6 parts of Crataegus, 10 parts of Scutellaria, 8 parts of Albizia Julibrissin, 5 parts of Lotus Seed Heart; S22, the first group was washed, air-dried, and then crushed to 120 mesh, and 10 times the amount of deionized water was added. The mixture was stirred and extracted at 60°C for 40 minutes, and the first extract was filtered; S23, the second group was washed, air-dried, and then crushed to 120 mesh, added with 10 times deionized water, extracted at 80°C for 2 hours, and filtered to obtain the second extract; S24. Combine the two extracts (volume ratio 1:1), remove impurities through fine filtration, and obtain 200 parts of auxiliary material extract.

[0038] S3, compounding steps: S31, pouring 120 parts of the bergamot extract and 200 parts of the auxiliary material extract into a stirring tank, starting the stirring equipment, and premixing at 100 rpm for 2 minutes to form a uniform liquid system; S32, add 400 parts of purified water and continue stirring until the solution is clear and uniform; S33. Add 6 parts of sucrose and 4 parts of edible flavor (a compound of bergamot flavor and mint flavor) in sequence while stirring, and continue stirring until completely dissolved to obtain a basic compound liquid.

[0039] S4, stabilization processing: Add 3 parts of stabilizer, wherein carrageenan and ammonium polyacrylate are premixed in a ratio of 0.8:1, and stir and homogenize for 15 minutes after addition to form a stable liquid phase structure.

[0040] S5. Sterilization treatment: Ultra-high temperature instantaneous sterilization (UHT) is used at a temperature of 95°C and the sterilization time is controlled within 15 seconds to ensure that the active ingredients are not degraded while killing microorganisms.

[0041] S6. Cooling and filling: After the beverage is sterilized and naturally cooled to room temperature, it is immediately filled and sealed to obtain a high-concentration functional plant beverage product.

[0042] Comparative Example 1 (C1) This comparative example 1 provides a plant beverage with bergamot as the main raw material. Its formula lacks a variety of mood regulating and tranquilizing ingredients, and does not contain important medicinal and edible auxiliary materials with neuroregulatory, sedative and anti-anxiety effects such as Polygala tenuifolia and Polygonum multiflorum, as a comparative example of the ingredients of the present invention.

[0043] By weight, its components are as follows: 100 parts of bergamot extract, 80 parts of auxiliary material extract, 2 parts of stabilizer, 350 parts of purified water, 5 parts of sucrose, and 3 parts of edible flavor.

[0044] Preparation method: S1. Preparation of bergamot extract: 80 parts of citron arborvitae, 80 parts of immature bitter orange, 60 parts of floating wheat, 60 parts of valerian, 80 parts of ganoderma lucidum, 60 parts of julibrissin bark, and 30 parts of lotus seed core were washed, dried, and crushed to 120 mesh size. After mixing, 10 times the volume of 80% ethanol was added, and the mixture was extracted at room temperature for 48 hours. The first liquid was filtered to obtain the first liquid; water was then added at a ratio of 1:10 and decocted for 1.5 hours to obtain the second extract; the two extracts were combined, concentrated, spray-dried, and redissolved to obtain the citron extract.

[0045] S2. Preparation of auxiliary material extract: S21, by weight, take 30 parts of jasmine tea, 5 parts of perilla leaves, 5 parts of liquorice, 10 parts of scutellaria, 10 parts of hawthorn, 10 parts of pomegranate flowers, and 10 parts of Panax notoginseng; wash the auxiliary materials in sequence, air-dry at low temperature, mix them evenly, and grind them to pass through a 120-mesh sieve; S22, low-temperature extraction: the mixed excipients were placed in an extraction container, deionized water was added, and the mass ratio of water to excipient powder was 10:1. The mixture was slowly stirred and extracted at 55° C. for 40 minutes, and filtered to obtain the first extract; S23, medium temperature extraction: add an equal amount of deionized water to the residue, maintain the temperature at 75°C, continue extraction for 1.5 hours, and filter again to obtain the second extract; S24, combining and filtering: combining the extracts of the first and second stages, letting them settle, taking the supernatant, filtering through a 100-mesh sieve to remove impurities, and concentrating to the target volume to finally prepare 80 parts of the auxiliary material extract.

[0046] S3, compounding and stabilization treatment: Mix the bergamot extract with the auxiliary material extract, pre-mix at low speed for 2 minutes, add water, sucrose and edible flavor and stir evenly, add 2 parts of stabilizer and homogenize, pasteurize at 85℃ for 30 minutes, fill and seal after cooling.

[0047] Comparative Example 2 (C2) This comparative example 2 provides a plant drink with bergamot as the main raw material, and the compatibility scheme lacks a variety of auxiliary materials with antidepressant and tranquilizing effects.

[0048] The components are as follows by weight: 90 parts of bergamot extract, 120 parts of auxiliary material extract, 2.5 parts of stabilizer, 360 parts of purified water, 5.5 parts of sucrose, and 3.5 parts of edible flavor.

[0049] The auxiliary material extract is extracted from the following plant raw materials: 30 parts of jasmine tea, 5 parts of perilla leaves, 6 parts of pomegranate flowers, 8 parts of lilies, 4 parts of Panax notoginseng, 4 parts of liquorice, 5 parts of hawthorn, and 3 parts of lotus seed core.

[0050] Preparation method: S1. Preparation of bergamot extract: Take 80 parts of bergamot arborvitae, 80 parts of immature bitter orange, 60 parts of floating wheat, 60 parts of valerian, 80 parts of ganoderma lucidum, 60 parts of polygala tenuifolia, and 60 parts of tuberose, wash, air-dry, and grind until they pass through a 120-mesh sieve, add 80% ethanol at a ratio of 1:10, and extract for 48 hours, then extract with water in an equal proportion for 1.5 hours, combine the extracts, concentrate and dry, and redissolve to obtain bergamot extract.

[0051] S2. Preparation of auxiliary material extract: The first group includes jasmine tea, perilla leaves, pomegranate flowers, and lilies; the mixture is washed, air-dried, and ground to pass through a 120-mesh sieve, deionized water is added, and the mass ratio of water to auxiliary material powder is 8-10:1. The mixture is extracted at 50-60°C for 30-40 minutes. After initial filtration and static precipitation, the supernatant is collected to obtain the first auxiliary material extract; The second group includes Panax notoginseng, licorice, hawthorn, and lotus seed core; the mixture is washed, air-dried, and ground to pass through a 120-mesh sieve, deionized water is added, and the mass ratio of water to auxiliary material powder is 8-10:1. The mixture is extracted at 70-80°C for 1.5-2 hours, and the supernatant is collected after coarse filtration and static sedimentation to obtain the second auxiliary material extract; The first section auxiliary material extract and the second section auxiliary material extract were mixed in a volume ratio of 1:1, and filtered through a 100-mesh sieve to remove impurities to obtain the auxiliary material extract; S3, compounding and stabilization: The bergamot extract and the auxiliary material extract are premixed, stirred at a low speed for 2 minutes, and water, sucrose and essence are added and stirred evenly. After adding 2.5 parts of stabilizer, the mixture is pasteurized, cooled and filled to obtain the finished product.

[0052] Comparative Example 3 (C3) Comparative Example 3 provides a plant drink with bergamot as the main raw material, but lacks a variety of auxiliary materials with antidepressant and tranquilizing effects in the compatibility scheme.

[0053] The formula of the drink is as follows (by weight): 100 parts of bergamot extract, 70 parts of auxiliary material extract, 2 parts of stabilizer (a mixture of carrageenan and ammonium polyacrylate, in a ratio of 0.6:1), 350 parts of purified water; 5 parts of sucrose; 3 parts of edible flavor (a bergamot + lemon compound flavor).

[0054] The preparation method is as follows: S1. Preparation of bergamot extract: Wash 50 parts of bergamot arborvitae, 30 parts of fructus aurantii, and 20 parts of dried tangerine peel, air-dry, and grind them into a 120-mesh sieve. Add 10 times the volume of 80% ethanol and extract at room temperature for 48 hours. Filter to obtain the first extract, then add 10 times the volume of water and boil for 1.5 hours to obtain the second liquid. Combine the two liquids, concentrate, spray-dry, and then redissolve to obtain the bergamot extract.

[0055] S2. Preparation of auxiliary material extract: Mix 20 parts of lemongrass, 15 parts of chrysanthemum, 10 parts of dried tangerine peel, 10 parts of honeysuckle, and 15 parts of mint, wash, dry, and grind into powder, extract with 8-10 times the volume of water in two stages: low temperature extraction at 55°C for 40 minutes and high temperature extraction at 75°C for 1.5 hours, combine, filter, and remove impurities to prepare 70 parts of auxiliary material extract.

[0056] S3, compounding and stabilization treatment: Add bergamot extract, auxiliary material extract, water, sucrose and essence into the mixing tank in sequence, premix until uniform, then add stabilizer and stir to form a uniform mixture.

[0057] S4, sterilization and filling: After pasteurization, the product is cooled to room temperature and then bottled to obtain the finished beverage.

[0058] Comparative Example 4 (C4) The beverage in this comparative example is a sedative and sleep-inducing compound botanical beverage based on traditional Chinese medicinal materials and does not contain bergamot extract. The main ingredients are as follows (in parts by weight): 60 parts of jujube seed extract, 40 parts of cypress seed extract, 40 parts of Polygonum multiflorum extract, 30 parts of lily extract, and 20 parts of julibrissin bark extract; 10 parts of polygala tenuifolia extract, 300 parts of water, 5 parts of sucrose, 2 parts of stabilizer (xanthan gum and pectin in a ratio of 1:1), and 3 parts of edible flavoring (mint flavor is used).

[0059] Preparation method: S1. Raw material pretreatment: Wash and air-dry the plant materials and then grind them into 100–120 mesh.

[0060] S2. Extraction process: A two-stage water extraction method was used: 10 times the volume of water was added, extraction was performed at 90°C for 1 hour, and the extraction was repeated once after filtration. The two extracts were combined, concentrated, and redissolved to the required concentration to obtain each single component extract.

[0061] S3. Compounding and homogenization: All extracts, water, sucrose, stabilizers and flavors were mixed according to the proportions, pre-mixed using a low-speed stirrer (300 rpm, 10 minutes), and then homogenized at high speed (3000 rpm, 5 minutes) to form a uniform solution.

[0062] S4, sterilization and filling: After pasteurization (80°C, 15 minutes), the product is cooled and then filled and packaged at room temperature to obtain the finished product.

[0063] Comparative experiment Experimental purpose: To verify whether the beverage of the present invention has significant tranquilizing, sleep-inducing and antidepressant effects in a mouse model.

[0064] Experimental animals: Species: Kunming mice (SPF grade), either sex; weight: 18–22 g; number: 10 per group; adaptation period: 7 days, housed under conventional conditions (22 ± 2°C, 50% ± 10% humidity, 12 h light / dark).

[0065] Depression model establishment: The chronic unpredictable stress (CUMS) model was used to induce depressive-like behavior in mice. The method was as follows: Continuous intervention duration: 21 days; 1–2 stimulation methods were selected daily, avoiding repetition and rotating the combination: Tilt cage (45°) for 12 h; moist bedding (50 mL of water) for 12 h; reversed day and night cycle; social isolation; fasting for 24 h and water deprivation for 24 h; physical stimulation (restraint, shaking).

[0066] After the modeling was completed, sucrose preference test and forced swimming test were used to confirm the success of the modeling.

[0067] The grouping details are shown in Table 1: Table 1 Comparative experimental group details Grouping Group number Dosage Instructions Blank control group Group N No modeling, no medication, only oral administration of an equal amount of purified water Normal saline group NS group After modeling, the rats were gavaged with normal saline (equal volume) Example Group 1 T1 group After modeling, the T1 drink of Example 1 was gavage Example Group 2 T2 group After modeling, gavage the Example T2 drink Example Group 3 T3 group After modeling, gavage the Example T3 drink Comparative Example Group 1 Group C1 After modeling, the control sample C1 drink was gavage Comparative Example Group 2 Group C2 After modeling, the control sample C2 drink was gavage Comparative Example Group 3 Group C3 After modeling, the control sample C3 drink was gavage Comparative Example Group 4 Group C4 After modeling, gavage the control sample C4 drink Dosing volume: calculated based on 10 mL / kg of mouse body weight; Dosage cycle: continuous gavage for 14 days Detection indicators and methods: 1. Behavioral testing (once on the 7th and 14th day after administration): Forced swim test (FST): The time mice spend immobile in water is recorded; a decrease in immobility time indicates an enhanced antidepressant effect.

[0068] Tail suspension test (TST): The time the mouse remains suspended in mid-air is recorded. The shorter the immobility time, the stronger the antidepressant effect.

[0069] Sucrose Preference Test (SPT): Assess loss of pleasure; decreased preference indicates worsening depression. The experimental method is: All animals are given two drinking bottles simultaneously, one containing 1% sucrose solution and the other containing pure water. They are allowed to habituate to the choice. After 24 hours, the consumption of the sucrose and water bottles is measured: .

[0070] 2. Sleep latency and total sleep time (12th day of administration) Induction method using sodium pentobarbital: Dose: 50 mg / kg ip; The time from injection to loss of righting reflex (sleep latency) of mice was observed; The time for righting reflex recovery was recorded and the total sleep time was calculated; The calming and sleep-aiding effect is measured by shortening the sleep latency and extending the total sleep time.

[0071] The experimental data are shown in Table 2: Table 2 Comparative experimental results Grouping FST immobility time (s) TST static time (s) Sucrose preference (%) Sleep latency (s) Total sleep time (min) Group N 97.2±8.3 91.5±7.9 86.1±3.4 102.5±9.2 58.4±4.1 NS group 164.3±12.6 159.7±10.4 58.7±5.2 165.2±10.8 33.6±3.9 T1 group 104.6±9.7 101.3±8.9 81.5±4.2 108.3±8.6 52.3±3.6 T2 group 117.9±10.3 112.7±9.4 76.2±4.9 120.7±9.3 47.1±4.4 T3 group 98.4±8.6 93.8±7.2 83.4±3.6 105.6±7.8 55.2±4.0 Group C1 138.6±11.2 132.7±10.1 66.5±4.7 142.3±10.2 41.4±3.7 Group C2 131.7±10.4 126.3±9.5 67.8±5.1 137.5±10.9 43.2±4.2 Group C3 143.2±11.6 137.9±10.7 62.9±5.5 145.4±9.8 38.9±3.8 Group C4 121.4±9.8 115.5±9.0 71.3±4.3 125.1±8.5 45.7±3.9 Data Analysis From the behavioral indicators, such as Figure 2 As shown in Figure 2, in the forced swimming test (FST) and the tail suspension test (TST), the blank control group (N group) showed a shorter immobility time. Figure 3 As shown, the sucrose preference was high, indicating a normal mental state. In contrast, the model control group (NS group) showed significantly prolonged immobility in both experiments (164.3 s for the FST and 159.7 s for the TST), and a significant decrease in sucrose preference to 58.7%, reflecting significant depressive-like behavior, confirming the successful establishment of the CUMS model.

[0072] In the embodiment group, Figure 2 As shown, the immobility times of the T1 and T3 groups on the FST and TST were significantly lower than those of the NS group (decreasing to 104.6 s and 98.4 s, respectively). Sucrose preference rebounded to 81.5% and 83.4%, approaching that of the blank group, demonstrating a strong antidepressant effect. The T2 group had an immobility time of 117.9 s (FST) and 112.7 s (TST), and a sucrose preference of 76.2%. While still superior to the control group, the magnitude of improvement was slightly lower than that of T1 and T3, suggesting a relatively low content or weaker proportion of active ingredients.

[0073] Comparative groups C1 to C4 all showed varying degrees of residual depressive symptoms, with groups C1 and C3 performing the worst, with immobility times of 138.6 s and 143.2 s, respectively, and sucrose preferences of only 66.5% and 62.9%, respectively. This indicates that in the absence of key ingredients such as Polygonum multiflorum and Polygala tenuifolia, or the absence of bergamot extract, the antidepressant effect was significantly insufficient.

[0074] In terms of sleep-related indicators, such as Figure 4 As shown in Figure 2, the sleep latency of mice in the NS group was significantly prolonged to 165.2 s, and the total sleep time was reduced to 33.6 min, further indicating that the model animals were in a state of significant neural excitement. The sleep latency of the T1 and T3 groups was significantly shortened (108.3 s and 105.6 s, respectively). Figure 5 As shown in the figure, total sleep time was extended to 52.3 minutes and 55.2 minutes, respectively, indicating that it has a good sleep-inducing and sedative effect. The latency of the T2 group was 120.7 seconds and the sleep time was 47.1 minutes, which is a moderate improvement.

[0075] Relatively speaking, the control group failed to significantly improve the sleep performance of the model animals. The total sleep time of groups C1 and C3 was less than 42 minutes, and the latency period exceeded 140 seconds. In particular, the performance of group C3 was close to that of the NS group, further confirming that drinks lacking synergistic tranquilizing components or with unreasonable combinations are unlikely to achieve a good sedative effect.

[0076] In summary, Examples T1 and T3 demonstrated optimal tranquilizing, sleep-inducing, and antidepressant effects, with behavioral tests and sleep parameters approaching normal levels and significantly outperforming the control groups. This demonstrates the synergistic effect of combining the bergamot extract with various medicinal and edible ingredients (such as Polygonum multiflorum, Polygala tenuifolia, Albizia julibrissin bark, and Nelumbo nucifera). This compound beverage exhibits significant advantages in mood regulation and neuroprotection, demonstrating promising potential for widespread application.

[0077] The basic principles, main features, and advantages of the present invention are shown and described above. Those skilled in the art should understand that the present invention is not limited to the foregoing embodiments. The foregoing embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and modifications may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and modifications are intended to fall within the scope of the present invention. The scope of protection claimed in the present invention is defined by the appended claims and their equivalents.

Claims

1. A drink with calming, sleep-inducing and anti-depressant effects, characterized in that: Calculated by weight, it includes the following components: 60–120 parts of bergamot extract, 150–200 parts of auxiliary material extract, 2–3 parts of stabilizer, 300–400 parts of water, 5–6 parts of sucrose, and 3–4 parts of edible flavoring.

2. A beverage with tranquilizing, sleep-inducing and anti-depressant effects according to claim 1, characterized in that: The bergamot extract is a mixed extract obtained by extracting the following plant raw materials in parts by weight: 80-100 parts of bergamot arborvitae, 80-100 parts of immature bitter orange, 60-80 parts of floating wheat, 60-80 parts of valerian, 80-100 parts of ganoderma lucidum, 60-80 parts of polygala tenuifolia, 60-80 parts of tuberose vine, 60-80 parts of lily, 60-80 parts of julibrissin bark, and 30-50 parts of lotus seed core.

3. A beverage with tranquilizing, sleep-inducing and anti-depressant effects according to claim 1, characterized in that: The auxiliary material extract is a mixed extract obtained by extracting the following plant raw materials in parts by weight: 30-40 parts of jasmine tea, 4-6 parts of perilla leaves, 5-7 parts of pomegranate flowers, 6-8 parts of lilies, 4-6 parts of Panax notoginseng, 4-5 parts of liquorice, 5-6 parts of hawthorn, 8-10 parts of scutellaria, 6-8 parts of julibrissin bark, and 3-5 parts of lotus seed core.

4. The beverage with tranquilizing, sleep-inducing and anti-depressant effects according to claim 1, characterized in that: The stabilizer is a mixture of carrageenan and ammonium polyacrylate, and the mixing ratio of carrageenan to ammonium polyacrylate is (0.5-0.8):

1.

5. The beverage with tranquilizing, sleep-inducing and anti-depressant effects according to claim 1, characterized in that: The edible flavor is selected from one of bergamot flavor, citrus flavor, jasmine flavor, mint flavor or compound flavors thereof.

6. The method for preparing the beverage having the effects of calming the mind, promoting sleep and combating depression according to any one of claims 1 to 5, characterized in that: The following steps are involved: S1, preparing a bergamot extract; taking a predetermined amount of bergamot extract raw material, crushing, extracting, drying, and dissolving to obtain a bergamot extract; S2. Preparation of auxiliary material extract: After the auxiliary material extract raw materials are grouped, low-temperature extraction and medium-temperature extraction are performed respectively to obtain auxiliary material extract; S3, compounding the bergamot extract, auxiliary material extract, water, sucrose, and edible flavor according to a predetermined ratio to obtain a basic compound solution; S4, stabilization treatment: adding 2-3 parts of a stabilizer to the base compound solution, wherein the stabilizer is a mixture of carrageenan and ammonium polyacrylate in a mixing ratio of (0.5-0.8):1, and stirring to obtain a mixed solution; S5, sterilization: pasteurizing or ultra-high temperature instantaneous sterilization the mixed solution; S6. Cooling and filling: The sterilized beverage is cooled to room temperature and filled to obtain the finished product.

7. The method for preparing a beverage having the effects of calming the mind, promoting sleep and combating depression according to claim 6, characterized in that: Step S1 specifically includes: S11. Ingredients: 80-100 parts of Citrus aurantium immaturus, 80-100 parts of Aralia citriodora, 60-80 parts of Triticum tiliaceum, 60-80 parts of Valerian root, 80-100 parts of Ganoderma lucidum, 60-80 parts of Polygala tenuifolia, 60-80 parts of Polygonum multiflorum, 60-80 parts of Lilium bulbs, 60-80 parts of Albizia julibrissin bark, and 30-50 parts of Lotus seed core, by weight; S12, grinding: washing and air-drying the raw materials in sequence, grinding them after air-drying, and passing them through a 120-mesh sieve to obtain raw material powder; S13, ethanol extraction: Place the raw material powder in an extraction tank, add 8-10 times the total weight of ethanol solution, with a concentration of 80%-90%, seal and extract for 48-62 hours. After the extraction is completed, let it stand and filter to separate the residue layer and the liquid layer; S14, water extraction: add 8-10 times the total weight of deionized water to the extraction tank, seal and extract for 48-62 hours, then let it stand and filter to obtain the second liquid layer; S15, concentration and drying: The liquid layers obtained in steps S3 and S4 are mixed, allowed to stand and precipitated twice, and the supernatant is collected, concentrated, and spray-dried to obtain a powdered extract; S16, dissolving: Weigh 60-120 parts of the powdered extract, add it to 600-700 parts of purified water, dissolve it, and stir it evenly.

8. The method for preparing a beverage having the effects of calming the mind, promoting sleep and combating depression according to claim 6, characterized in that: Step S2 specifically includes: S21. Grouping and taking of auxiliary materials: Divide the excipients into two groups: The first group included: 30–40 parts of jasmine tea, 4–6 parts of perilla leaves, 5–7 parts of pomegranate flowers, and 6–8 parts of lily; The second group included: 4–6 parts of Panax notoginseng, 4–5 parts of Licorice, 5–6 parts of Crataegus, 8–10 parts of Scutellaria, 6–8 parts of Albizia Julibrissin, and 3–5 parts of Lotus Seed Heart; S22, first group extraction process: The first group of auxiliary materials were washed and air-dried in sequence, and then ground into powder until they passed through a 120-mesh sieve; Take the first group of powders and add them into deionized water with a volume of 8–10 times the total weight, and extract them by slow stirring at 50–60°C for 30–40 minutes; After completion, coarse filter and let it settle, take the supernatant and mark it as the first auxiliary material extract for later use; S23, second group extraction process: The second group of auxiliary materials were washed, air-dried and then pulverized to pass through a 120-mesh sieve; Add 8–10 times the weight of deionized water and extract at 70–80°C for 1.5–2 hours with intermittent stirring; After completion, coarse filter and let stand to settle, take the supernatant and mark it as the second auxiliary material extract; S24, auxiliary material liquid merging: The first auxiliary material extract and the second auxiliary material extract were combined in a ratio of 1:1, filtered to remove impurities, and then stirred to obtain an auxiliary material extract.

9. The method for preparing a beverage having the effects of calming the mind, promoting sleep and combating depression according to claim 6, characterized in that: Step S3 specifically includes: S31, compounding treatment: placing 60-120 parts of the bergamot extract and 150-200 parts of the auxiliary material extract into a stirring tank, turning on the stirring device, and premixing at a low speed of 50-100 rpm for 1-2 minutes to preliminarily mix the bergamot extract and the auxiliary material extract to form a stable liquid phase base; S32, adding 300-400 parts of the predetermined purified water to the stable liquid phase base, and continuing to stir until the system is uniform and transparent; S33. While stirring, add 5-6 parts of sucrose and 3-4 parts of edible flavoring in sequence, and continue stirring until all ingredients are fully dissolved and mixed to form a basic compound liquid.

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