Application of animal bifidobacterium subsp. Lactis MN-Gup or composition thereof in oral beauty

Abstract

The invention discloses application of animal bifidobacterium subsp. Lactis MN-Gup or a combination of the animal bifidobacterium subsp. Lactis MN-Gup in oral cosmetology, provides new application of the animal bifidobacterium subsp. Lactis MN-Gup, and particularly relates to application of the animal bifidobacterium subsp. Lactis MN-Gup in oral cosmetology for brightening skin color, resisting light aging and the like. The invention further provides an oral beautifying composition, and galactooligosaccharide and bifidobacterium animalis subsp. Lactis MN-Gup are matched with each other according to a specific proportion, so that the oral beautifying composition has a synergistic effect on brightening skin color, resisting light aging and the like.

Description

Technical Field

[0001] The present invention relates to a new application field of animal Bifidobacterium lactis subspecies MN-Gup, and in particular to application of animal Bifidobacterium lactis subspecies MN-Gup or a composition thereof in oral beauty. Background Art

[0002] Currently, the primary treatments for skin problems like photoaging and pigmentation are skincare products and facial masks. However, these methods often fail to fundamentally alter the biological processes of skin aging and carry potential side effects, such as hormonal imbalances. Due to these potential side effects, the approach to improving skin problems is shifting towards more natural methods, such as lifestyle modifications, dietary adjustments, and the use of safer natural compounds and nutritional supplements. Oral beauty can improve skin condition from within by ingesting bioactive ingredients, enhancing metabolism and promoting health and balance, rather than simply addressing the external manifestations of skin problems.

[0003] Probiotics are living microorganisms that, when consumed in sufficient quantities, can have beneficial effects on the host's health. They can regulate intestinal flora, improve the intestinal barrier, modulate immune responses, and regulate metabolism, among other things, to promote gastrointestinal health, weight loss, alleviate diabetes, alleviate depression, and improve women's health. Some skincare products now incorporate probiotics, primarily to repair the skin barrier and improve eczema, acne, and other issues, but research on probiotics for oral beauty is limited.

[0004] Bifidobacterium animalis subsp. lactis MN-Gup, originating from the Longevity Village of Bama, Guangxi, has a deposit number of CGMCC No. 15578 and was deposited on April 10, 2018, at the General Microbiology Center of the China Culture Collection Administration, Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, 100101, China. Currently, the publicly available uses of Bifidobacterium animalis subsp. lactis MN-Gup are limited to laxative effects, restoring intestinal flora health, improving obesity, and alleviating type 2 diabetes. Therefore, expanding new applications for Bifidobacterium animalis subsp. lactis MN-Gup is crucial. Summary of the Invention

[0005] Therefore, the object of the present invention is to provide a new use of animal Bifidobacterium lactis subsp. MN-Gup or a composition thereof, specifically, to provide the use of animal Bifidobacterium lactis subsp. MN-Gup or a composition thereof in the preparation of oral beauty products with brightening skin tone, anti-photoaging effects, etc.

[0006] In the first aspect, the present invention provides an application of animal Bifidobacterium lactis subsp. MN-Gup in the preparation of an oral beauty product. Specifically, the present invention provides an application of animal Bifidobacterium lactis subsp. MN-Gup in the preparation of an oral beauty product for brightening the skin tone, or provides an application of animal Bifidobacterium lactis subsp. MN-Gup in the preparation of an oral beauty product for anti-photoaging effect.

[0007] In a second aspect, the present invention provides an application of a composition of Bifidobacterium animalis subsp. lactis MN-Gup in the preparation of an oral beauty product. Specifically, the present invention provides an application of the composition in the preparation of an oral beauty product for brightening the skin tone, or provides an application of the composition in the preparation of an oral beauty product for anti-photoaging effect.

[0008] The composition comprises galacto-oligosaccharide and Bifidobacterium animalis subspecies lactis MN-Gup.

[0009] The mass ratio of the animal Bifidobacterium lactis subspecies MN-Gup to galacto-oligosaccharides (GOS) is 1:(0.5-50), for example, it can be 1:0.5, 1:1, 1:3, 1:5, 1:7, 1:9, 1:10, 1:15, 1:20, 1:25, 1:30, 1:35, 1:40, 1:45, 1:50 or any ratio therein not shown.

[0010] The number of viable bacteria of animal Bifidobacterium lactis subspecies MN-Gup in the composition is 3×10 9 CFU / g or more, for example: 3×10 9 CFU / g, 4×10 9 CFU / g, 5×10 9 CFU / g, 6×10 9 CFU / g, 7×10 9 CFU / g, 8×10 9 CFU / g, 9×10 9 CFU / g, 10×10 9 CFU / g, 20×10 9 CFU / g, 30×10 9 CFU / g, 50×10 9 CFU / g, 80×10 9 CFU / g, 100×10 9 CFU / g, 150×10 9 CFU / g, 200×10 9 CFU / g, 250×10 9 CFU / g, 300×10 9 CFU / g, 350×10 9 CFU / g, 400×10 9CFU / g, 450×10 9 CFU / g, 500×10 9 CFU / g, 600×10 9 CFU / g or any viable cell count therein, etc. or a content not shown.

[0011] The number of viable bacteria of Bifidobacterium animalis subspecies lactis MN-Gup in the composition is preferably 3×10 9 ~500×10 9 CFU / g, more preferably 3×10 9 ~150×10 9 CFU / g.

[0012] The technical solution of the present invention has the following advantages:

[0013] 1. The present invention provides a new use of Bifidobacterium animalis subsp. lactis MN-Gup or a composition thereof, specifically, the use of Bifidobacterium animalis subsp. lactis MN-Gup or a composition thereof in the preparation of an oral beauty product with skin-lightening and anti-photoaging effects.

[0014] 2. In the composition of animal Bifidobacterium lactis subspecies MN-Gup provided by the present invention, oligosaccharides and MN-Gup bacterial powder are combined with each other in a mass ratio of (0.5-50):1, which has a synergistic effect in brightening skin tone and anti-photoaging effects.

[0015] 3. The ingredients of the solution of the present invention are simple and easy to obtain, do not contain traditional Chinese medicine ingredients, are highly safe, and are easy to adjust in taste. It can be widely used in various products such as oral beauty products. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0017] Figure 1 This is a graph showing the experimental results of skin lightening efficacy in Example 1 of the present invention;

[0018] Figure 2 1 is a graph showing the experimental results of the anti-photoaging effect in Example 1 of the present invention;

[0019] Figure 3 This is a graph showing the experimental results of skin lightening efficacy in Example 2 of the present invention;

[0020] Figure 4This is a graph showing the experimental results of the anti-photoaging effect in Example 2 of the present invention. DETAILED DESCRIPTION

[0021] The following examples are provided for a better understanding of the present invention and are not intended to limit the best mode of implementation. They do not limit the content and scope of protection of the present invention. Any product identical or similar to the present invention obtained by anyone under the guidance of the present invention or by combining the features of the present invention with other prior arts shall fall within the scope of protection of the present invention.

[0022] If no specific experimental steps or conditions are specified in the examples, the conventional experimental steps or conditions described in the literature in this field can be used. If the manufacturer of the reagents or instruments is not specified, they are all commercially available conventional reagents.

[0023] Example 1

[0024] Application of Bifidobacterium animalis subsp. lactis MN-Gup in skin brightening and anti-photoaging.

[0025] The animal Bifidobacterium lactis subspecies MN-Gup in the present invention was provided by Mengniu High-Tech Dairy (Beijing) Co., Ltd., and its source is Bama Longevity Village in Guangxi. Its preservation number is CGMCC No. 15578. It was deposited in the General Microbiology Center of China Culture Collection Administration on April 10, 2018, address: No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postal Code: 100101. The viable cell count of MN-Gup powder used in this experiment was 2×10 11 CFU / g.

[0026] Application effect detection:

[0027] 1. Experimental Animals

[0028] Zebrafish were kept in fish farming water at 28°C (water quality: 200 mg of instant sea salt was added to each liter of reverse osmosis water, conductivity was 450-550 μS / cm; pH was 6.5-8.5; hardness was 50-100 mg / LCaCO3).

[0029] 2. Detection method

[0030] 2.1. Maximum detectable concentration (MTC) determination

[0031] Skin-lightening efficacy MTC assay: 3-day post-fertilization (dpf) zebrafish (Albino) with a melanin allele mutation were randomly selected and plated in 6-well plates, with 30 zebrafish treated in each well (experimental group). Each well was treated with different final concentrations of live MN-Gup bacteria (concentrations shown in Table 1). A control group and a model group were also established, with a volume of 3 mL per well. After treatment at 28°C for 2 hours, all experimental groups except the control group were treated with menadione in water to establish a zebrafish pigmentation model. After treatment at 28°C for an additional 22 hours, the MTC of the samples in the model zebrafish was measured.

[0032] Anti-photoaging efficacy MTC assay: Wild-type AB zebrafish (3 days post-fertilization (dpf)) were randomly selected and plated in 6-well plates, with 30 zebrafish treated in each well (experimental group). Each well was treated with different final concentrations of live MN-Gup bacteria (see Table 2 for concentrations). Both normal and model groups were set up, with a volume of 3 mL per well. After treatment at 28°C for 3 hours, all experimental groups except the normal group were exposed to simulated sunlight to establish a zebrafish tail fin wrinkling model. After treatment at 28°C for another day, the MTC of the samples in the model zebrafish was measured.

[0033] 2.2 Evaluation of the skin-lightening effect of Bifidobacterium animalis subsp. lactis MN-Gup

[0034] 3dpf melanin allele mutant Albino zebrafish were randomly selected and plated in 6-well plates, with 30 zebrafish treated in each well (experimental group). Water-soluble samples were administered and MN-Gup powder was administered to three dose groups: low-dose MN-Gup group (MN-Gup-L), medium-dose MN-Gup group (MN-Gup-M), and high-dose MN-Gup group (MN-Gup-H). The MN-Gup low-dose group received 1×10 6 CFU / mL, the intervention dose of the MN-Gup medium-dose group was 1×10 7 CFU / mL, and the intervention dose of the MN-Gup high-dose group was 1×10 8 CFU / mL. A normal group and a model group (MC) were set up at the same time, with a volume of 3 mL per well. After treatment at 28°C for 2 hours, all experimental groups except the normal group were given water-soluble menadione to establish a zebrafish pigmentation model. After treatment at 28°C for another 22 hours, 10 zebrafish were randomly selected from each experimental group and photographed under a dissecting microscope. Data were analyzed and collected using NIS-Elements D3.20 advanced image processing software. The zebrafish yolk sac pigment signal intensity was analyzed, and the statistical analysis results of this indicator were used to evaluate the skin lightening effect of the samples. Statistical analysis results were expressed as mean ± SE, and statistical analysis was performed using SPSS26.0 software. P < 0.05 indicated that the difference was statistically significant.

[0035] 2.3 Evaluation of the anti-photoaging effect of Bifidobacterium animalis subsp. lactis MN-Gup

[0036] Wild-type AB zebrafish were randomly selected at 3 dpf and plated in 6-well plates, with 30 zebrafish treated in each well. Water-soluble zebrafish were administered and treated with MN-Gup powder, divided into three dose groups: low-dose MN-Gup group (MN-Gup-L), medium-dose MN-Gup group (MN-Gup-M), and high-dose MN-Gup group (MN-Gup-H). The intervention dose of MN-Gup in the low-dose group was 1×10 6 CFU / mL, the intervention dose of the MN-Gup medium-dose group was 1×10 7 CFU / mL, and the intervention dose of the MN-Gup high-dose group was 1×10 8 CFU / mL. A normal group and a model group were set up simultaneously, with a volume of 3 mL per well. After treatment at 28°C for 3 hours, all experimental groups except the normal group were exposed to simulated sunlight to establish a zebrafish tail fin wrinkling model. After treatment at 28°C for another day, 10 zebrafish were randomly selected from each experimental group and photographed under a dissecting microscope. Data were analyzed and collected using NIS-Elements D 3.20 advanced image processing software. The zebrafish tail fin area was analyzed, and the statistical analysis results of this indicator were used to evaluate the anti-photoaging efficacy of the samples. Statistical results are expressed as mean ± SE. Statistical analysis was performed using SPSS 26.0 software. A p < 0.05 indicated statistical significance.

[0037] 3. Experimental results

[0038] 3.1. Maximum detectable concentration (MTC) determination results are shown in Tables 1 and 2 below.

[0039] Table 1

[0040]

[0041] Table 2

[0042]

[0043] MN-Gup bacterial powder at 1×10 5 CFU / mL~1×10 8 CFU / mL intervention dose range, the state of zebrafish in the experimental group was similar to that of the normal group, while 1×10 9 Five zebrafish died in the CFU / mL group, with a mortality rate of 17%. The anti-photoaging effect was moderate at 1×10 9 In the CFU / mL group, 12 zebrafish died, with a mortality rate of 40%. Therefore, the MTC of MN-Gup powder for brightening skin and anti-photoaging under the experimental conditions was 1×10 8CFU / mL.

[0044] 3.2. Experimental results on the skin lightening effect of Bifidobacterium animalis subsp. lactis MN-Gup Figure 1 shown.

[0045] Figure 1 * indicates significant difference compared with the model group (P<0.05). Figure 1 The yolk sac pigment signal intensity in the normal group was 324±0.13 million pixels, while that in the model group was 479±0.19 million pixels. The yolk sac pigment signal intensity in the model group was significantly higher than that in the normal group, indicating that the pigment deposition model was successfully established. The yolk sac pigment signal intensity in the MN-Gup-L, MN-Gup-M, and MN-Gup-H groups was 331±0.19 million pixels, 321±0.21 million pixels, and 320±0.18 million pixels, respectively, significantly lower than that in the model group. This suggests that low, medium, and high doses of MN-Gup can improve menadione-induced pigmentation and brighten skin tone.

[0046] 3.3. Experimental results on the anti-photoaging efficacy of Bifidobacterium animalis subsp. lactis MN-Gup Figure 2 shown.

[0047] like Figure 2 As shown, the caudal fin area of ​​the normal group was 868.4±11,800 pixels, while that of the model group was 613.4±32,300 pixels. The caudal fin area of ​​the model group was significantly lower than that of the normal group, indicating that simulated sunlight exposure caused caudal fin wrinkling in zebrafish, and the photoaging model was successfully established. The caudal fin areas of the MN-Gup-L group, MN-Gup-M group, and MN-Gup-H group were 728.1±12,900 pixels, 738.2±09,100 pixels, and 751.0±13,300 pixels, respectively, significantly higher than those of the model group. This suggests that low, medium, and high doses of MN-Gup can improve caudal fin wrinkling caused by simulated sunlight exposure and have anti-photoaging effects.

[0048] Example 2

[0049] Application of an animal Bifidobacterium lactis subsp. MN-Gup composition in the preparation of products with skin-lightening and anti-photoaging effects.

[0050] The animal Bifidobacterium lactis subsp. MN-Gup composition of the present invention comprises different proportions of galacto-oligosaccharides and MN-Gup bacterial powder in compositions 1-4; the MN-Gup bacterial powder used in this embodiment is the same as that in Example 1, which is provided by Mengniu High-Tech Dairy (Beijing) Co., Ltd., and the viable bacterial count of the MN-Gup bacterial powder is 2×10 11CFU / g; galacto-oligosaccharides (GOS) used in the experiment were purchased from Great Ocean Ingredients PTY LTD. In this example, the mass ratio of galacto-oligosaccharides (GOS) to MN-Gup bacterial powder in Composition 1 was 0.5:1, the mass ratio in Composition 2 was 50:1, the mass ratio in Composition 3 was 0.01:1, and the mass ratio in Composition 4 was 100:1.

[0051] Skin brightening and anti-photoaging effect testing:

[0052] 1. Experimental Animals

[0053] Zebrafish were kept in fish farming water at 28°C (water quality: 200 mg of instant sea salt was added to each liter of reverse osmosis water, conductivity was 450-550 μS / cm; pH was 6.5-8.5; hardness was 50-100 mg / LCaCO3).

[0054] 2. Detection method

[0055] The animal Bifidobacterium lactis subspecies MN-Gup composition is a combination of galacto-oligosaccharide and animal Bifidobacterium lactis subspecies MN-Gup. The present invention uses compositions with different compounding ratios to verify their effects in brightening skin tone and anti-photoaging. Specifically, compound group 1 (FP1) uses composition 1, compound group 2 (FP2) uses composition 2, compound group 3 (FP3) uses composition 3, and compound group 4 (FP4) uses composition 4.

[0056] 2.1. Determination of maximum detectable concentration (MTC).

[0057] In addition to MN-Gup, the animal Bifidobacterium lactis subsp. lactis MN-Gup composition also contains galactoligosaccharides; therefore, the MTC of galactoligosaccharides was obtained by the same method as the MTC determination of skin lightening efficacy and the MTC determination of anti-photoaging efficacy in Example 1; wherein, the concentration of the water-soluble galactoligosaccharide sample in the MTC determination of skin lightening efficacy is shown in Table 3 below, and the concentration of the water-soluble galactoligosaccharide sample in the MTC determination of anti-photoaging efficacy is shown in Table 4 below.

[0058] 2.2. Evaluation of the skin-lightening effect of the Bifidobacterium animalis subsp. lactis MN-Gup composition.

[0059] 3-day-old zebrafish of the Albino strain with a melanin allele mutation were randomly selected and plated in 6-well plates. Thirty zebrafish were treated in each well (experimental group). Samples FP1-FP4 were administered with water solution. A normal control group (NC), a model group (MC), a MN-Gup group, and a GOS group were also established. The volume per well was 3 mL, and the total concentration of the intervention sample in each group was 200 μg / mL. After 2 hours of treatment at 28°C, all experimental groups except the NC group were administered with menadione in water solution to establish a zebrafish pigmentation model. After a further 22 hours of treatment at 28°C, 10 zebrafish were randomly selected from each experimental group and photographed under a dissecting microscope. Data were collected and analyzed using NIS-Elements D 3.20 advanced image processing software. The zebrafish yolk sac pigment signal intensity was analyzed, and the skin-lightening efficacy of the samples was evaluated using statistical analysis of this indicator. Statistical results are expressed as mean ± SE and analyzed using SPSS 26.0 software. P < 0.05 indicated statistical significance.

[0060] 2.3. Evaluation of the anti-photoaging effect of the Bifidobacterium animalis subsp. lactis MN-Gup composition.

[0061] Wild-type AB zebrafish (3 dpf) were randomly selected and plated in 6-well plates, with 30 zebrafish treated per well. Samples FP1-FP4 were administered with water solution, respectively. A normal control group (NC), a model group (MC), a MN-Gup group, and a GOS group were also established. The volume per well was 3 mL, and the total concentration of the intervention sample in each group was 200 μg / mL. After treatment at 28°C for 3 hours, all experimental groups except the NC group were exposed to simulated sunlight to establish a zebrafish caudal fin wrinkling model. After treatment at 28°C for another day, 10 zebrafish were randomly selected from each experimental group and photographed under a dissecting microscope. Data were analyzed and collected using NIS-Elements D 3.20 advanced image processing software. The caudal fin area of ​​the zebrafish was analyzed, and the anti-wrinkle efficacy of the samples was evaluated using statistical analysis. Statistical results are expressed as mean ± SE. Statistical analysis was performed using SPSS 26.0 software. P < 0.05 indicated statistical significance.

[0062] 3. Experimental results

[0063] 3.1. The results of the maximum detectable concentration (MTC) determination are shown in Tables 3 and 4 below.

[0064] Table 3

[0065]

[0066] Table 4

[0067]

[0068] From the above results, it can be seen that when the intervention range of galactoligosaccharide is 125-2000 μg / mL, the state of zebrafish in the experimental group is similar to that of the normal group. Therefore, under the conditions of this experiment, the MTC of galactoligosaccharide in brightening skin color and anti-photoaging effect is 2000 μg / mL.

[0069] 3.2. Experimental results of the skin lightening effect of Bifidobacterium animalis subsp. lactis MN-Gup composition Figure 3 shown.

[0070] Figure 3 In the data, *: significant difference compared with the model group (MC) (P < 0.05); #: significant difference compared with the MN-Gup group (P < 0.05); &: significant difference compared with the GOS group (P < 0.05). The yolk sac pigment signal intensity in the normal group (NC) was 249 ± 0.09 million pixels, while that in the model group (MC) was 372 ± 0.16 million pixels. The yolk sac pigment signal intensity in the model group (MC) was significantly higher than that in the normal group (NC), indicating that the pigment deposition model was successfully established.

[0071] The yolk sac pigment signal intensity in the MN-Gup group was 2.75±0.14 million pixels, and in the GOS group was 2.98±0.13 million pixels, significantly lower than that in the model group. This suggests that at an intervention concentration of 200 μg / mL, both MN-Gup and GOS have a skin-lightening effect. The yolk sac signal intensity in the FP1 and FP2 groups was 2.05±0.09 million pixels and 2.48±0.10 million pixels, respectively, significantly lower than that in the model group and significantly lower than that in the MN-Gup and GOS groups. The yolk sac signal intensity in the FP3 and FP4 groups was 2.87±0.13 million pixels and 2.93±0.11 million pixels, respectively, significantly lower than that in the model group but not significantly different from that in the MN-Gup and GOS groups. This demonstrates that MN-Gup and GOS have a synergistic effect within a ratio range of 1:0.5 to 50.

[0072] 3.3. Experimental results of the anti-photoaging efficacy of Bifidobacterium animalis subsp. lactis MN-Gup composition Figure 4 shown.

[0073] Figure 4 In the data, *: significant difference compared with the model group (MC) (P < 0.05); #: significant difference compared with the MN-Gup group (P < 0.05); &: significant difference compared with the GOS group (P < 0.05). The caudal fin area of ​​the normal group (NC) was 798.7 ± 10,000 pixels, while that of the model group (MC) was 565.6 ± 13,600 pixels. The caudal fin area of ​​the model group (MC) was significantly lower than that of the normal group (NC), indicating that simulated sunlight exposure caused caudal fin wrinkling in zebrafish, and the photoaging model was successfully established.

[0074] The caudal fin area in the MN-Gup group was 64.42 ± 14,900 pixels, and the caudal fin area in the GOS group was 63.65 ± 15,500 pixels, both significantly higher than the model group. This suggests that at an intervention concentration of 200 μg / mL, both MN-Gup and GOS have anti-photoaging effects. The caudal fin areas in the FP1 and FP2 groups were 70.37 ± 16,100 pixels and 69.90 ± 13,800 pixels, respectively, significantly higher than the model group and significantly higher than the MN-Gup and GOS groups. The caudal fin areas in the FP3 and FP4 groups were 63.90 ± 1,500 pixels and 64.52 ± 1,600 pixels, respectively, significantly higher than the model group but not significantly different from the MN-Gup and GOS groups. This suggests a synergistic effect between MN-Gup and GOS within a ratio range of 1:0.5 to 50.

[0075] Obviously, the above embodiments are merely examples for clarity of explanation and are not intended to limit the implementation methods. Those skilled in the art will readily appreciate that other variations or modifications based on the above descriptions are possible. It is not necessary and impossible to enumerate all implementation methods here. Obvious variations or modifications arising therefrom remain within the scope of protection of the present invention.

Claims

1. Application of Bifidobacterium animalis subsp. lactis MN-Gup in the preparation of oral beauty products.

2. The use according to claim 1, characterized in that The oral cosmetic product is an oral cosmetic product for brightening skin tone.

3. The use according to claim 1, characterized in that The oral cosmetic product is an oral cosmetic product for resisting photoaging.

4. Use of a composition of Bifidobacterium animalis subsp. lactis MN-Gup in the preparation of oral cosmetic products.

5. The use according to claim 4, characterized in that The oral cosmetic product is an oral cosmetic product for brightening skin tone.

6. The use according to claim 4, characterized in that The oral cosmetic product is an oral cosmetic product for resisting photoaging.

7. The use according to any one of claims 4 to 6, characterized in that The composition comprises galacto-oligosaccharide and Bifidobacterium animalis subspecies lactis MN-Gup.

8. The use according to claim 7, characterized in that The number of viable bacteria of animal Bifidobacterium lactis subspecies MN-Gup in the composition is 3×10 9 CFU / g and above.

9. The use according to claim 7 or 8, characterized in that The animal Bifidobacterium lactis subspecies MN-Gup is contained in MN-Gup bacterial powder, and the mass ratio of MN-Gup bacterial powder to galacto-oligosaccharide is 1:(0.5-50).

10. The use according to claim 9, characterized in that The number of viable bacteria of animal Bifidobacterium lactis subspecies MN-Gup in the composition is 3×10 9 ~500×10 9 CFU / g.

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