Female private part antibacterial composition

Abstract

The invention belongs to the technical field of antibacterial products, and particularly relates to a female private part antibacterial composition. Through the synergistic effect of the white willow bark extract, the melaleuca alternifolia leaf oil, the sandalwood essential oil and the cedar wood oil, the prepared antibacterial composition has an excellent antibacterial effect, is mild and non-irritant, and can solve the problems that an existing female private part antibacterial agent is limited in antibacterial effect, and the antibacterial effect is poor; and gynecological diseases can be aggravated after long-term use. Wherein the white willow bark extract is prepared through enzymolysis of a compound enzyme composed of cellulase, feruloyl esterase and alkaline protease, the enzymolysis efficiency can be improved, and then the release rate of active ingredients in white willow bark is increased. The preparation method of the antibacterial composition is simple and convenient in operation process and suitable for industrial production. The antibacterial composition is applied to preparation of female private part care products and has good application value.

Description

Technical Field

[0001] The present invention belongs to the technical field of antibacterial products, and in particular relates to an antibacterial composition for female private parts. Background Art

[0002] The female private area (e.g., the vagina) is a unique habitat for bacteria, housing a diverse flora, dominated by Lactobacillus, a beneficial bacterium that breaks down glycogen in vaginal epithelial cells, maintaining a healthy, acidic vaginal environment. The health of the female private area is influenced not only by physiology, reproductive health, and hygiene, but also by the stresses of modern work and life, as well as unhealthy habits like staying up late and lacking exercise. Women are particularly susceptible to various gynecological conditions.

[0003] Gynecological inflammation is a common gynecological disease, primarily caused by an imbalance in vaginal flora, leading to inflammation of the female reproductive organs. Currently, there are many medications available to treat various gynecological inflammations, primarily oral and topical. However, oral medications can be irritating to the stomach, while topical medications, such as vaginal antibacterial agents, are often formulated using a combination of highly bactericidal chemical ingredients such as chlorhexidine gluconate and benzalkonium chloride with plant-derived raw materials, aiming to maintain antibacterial efficacy while reducing skin irritation.

[0004] However, the combination of the above-mentioned antibacterial agents usually has limited antibacterial effects and cannot achieve rapid antibacterial effects. Long-term use can also lead to disturbances in the flora of the private parts and may also cause problems such as aggravated itching and dryness. Therefore, providing a female private part antibacterial product with excellent antibacterial properties and a mild and non-irritating effect is an urgent problem to be solved. Summary of the Invention

[0005] In response to the above defects or improvement needs in the prior art, the present invention provides a female private part antibacterial composition to solve the problem that the existing female private part antibacterial agents have limited antibacterial effects and long-term use will aggravate gynecological diseases.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides an antibacterial composition for female private parts, comprising the following components by weight: 12-20 parts of white willow bark extract, 8-16 parts of Melaleuca alternifolia leaf oil, 9-15 parts of sandalwood essential oil, and 8-14 parts of cedar wood oil; a method for preparing the white willow bark extract comprises the following steps:

[0008] S1. Taking willow bark powder, and mixing the willow bark powder and distilled water in a certain proportion to obtain a mixed solution I;

[0009] S2, adding a complex enzyme to the mixed solution I, performing microwave treatment, and then performing constant temperature enzymolysis treatment to obtain an enzymolysis solution;

[0010] S3, inactivating the enzyme, filtering, and concentrating under reduced pressure on the enzymatic hydrolyzate to obtain a willow bark extract;

[0011] Preferably, the antibacterial composition comprises the following components in parts by weight: 16 parts of white willow bark extract, 12 parts of Melaleuca alternifolia leaf oil, 12 parts of sandalwood essential oil, and 11 parts of cedar wood oil.

[0012] Preferably, the complex enzyme consists of cellulase, ferulic acid esterase and alkaline protease in a mass ratio of 2-3:0.5-1:0.5.

[0013] Preferably, the antibacterial composition further comprises the following components in parts by weight: 2-6 parts of oligofructose and 1-5 parts of inulin.

[0014] Preferably, in step S1, the ratio of the willow bark powder to distilled water is 1 g: 10-20 mL.

[0015] Preferably, the total amount of the complex enzyme added in step S2 is 1.5-2.5% of the mass of the white willow bark.

[0016] Preferably, the complex enzyme consists of cellulase, ferulic acid esterase and alkaline protease in a mass ratio of 2.5:0.7:0.5.

[0017] Preferably, the microwave power in step S2 is 300-400 W, and the microwave time is 10-20 min.

[0018] Preferably, the enzymatic hydrolysis temperature in step S2 is 50-60° C., the enzymatic hydrolysis pH is 5.0-6.0, and the enzymatic hydrolysis time is 1-2 h.

[0019] Preferably, the enzyme inactivation temperature in step S3 is 75-85° C., and the enzyme inactivation time is 10-20 min.

[0020] Preferably, the step S3 adopts high-speed centrifugal filtration, the centrifugal speed is 8000 r / min, and the centrifugal time is 20 min.

[0021] Preferably, the reduced pressure concentration in step S3 is concentrated under reduced pressure to 30% of the original volume at a pressure of 0.07 MPa.

[0022] In a second aspect, the present invention provides a method for preparing an antibacterial composition, comprising the following steps:

[0023] 1) mixing willow bark extract, oligofructose, inulin, and water, stirring and heating to 50-60° C. until completely dissolved to obtain mixture I;

[0024] 2) mixing Melaleuca alternifolia leaf oil, sandalwood essential oil, cedarwood oil, and an emulsifier to obtain a mixture II;

[0025] 3) Mix mixture I and mixture II, stir and heat to 40-50°C, and mix well to obtain the product.

[0026] In the step 1), the weight ratio of water to the sum of the weight of oligofructose and inulin is 1:1.

[0027] The emulsifier is at least one of PPG-26-butane ether-26, PEG-40 hydrogenated castor oil and lecithin, and the added amount of the emulsifier is 5-10% of the total mass of the melaleuca alternifolia leaf oil, sandalwood essential oil and cedarwood oil.

[0028] In a third aspect, the present invention provides use of an antibacterial composition in the preparation of female private parts care products.

[0029] Beneficial effects of the present invention:

[0030] (1) The present invention provides an antibacterial composition for female private parts, which comprises white willow bark extract, Melaleuca alternifolia leaf oil, sandalwood essential oil and cedarwood oil. Among them, the main active ingredient of white willow bark extract is salicin, which has the effects of clearing heat, relieving pain, anti-inflammatory, delaying skin aging, and antibacterial; the main component of Melaleuca alternifolia leaf oil is monoterpene compounds, which has significant antibacterial activity, antiviral effect and antioxidant effect, and is a highly active natural antibacterial agent; sandalwood essential oil has antibacterial and antiviral, anti-inflammatory and antioxidant effects due to its rich content of effective active ingredients such as sesquiterpenoids, santalene, and bisabolol; cedarwood oil contains active substances such as cedrol, cedrone and atlantone, which has the effects of oil control, antibacterial and anti-inflammatory, and moisturizing and repairing. The research results of the present invention show that the combined use of white willow bark extract, Melaleuca alternifolia leaf oil, sandalwood essential oil and cedarwood oil has a significant synergistic effect, and the composition has a significant antibacterial effect and a mild and non-irritating effect.

[0031] (2) The present invention uses a composite enzyme composed of cellulase, ferulic acid esterase and alkaline protease to enzymatically hydrolyze the white willow bark. Alkaline protease can hydrolyze the structural protein in the plant cell wall, loosen the cell wall, and promote the release of salicin in the white willow bark. Ferulic acid esterase can hydrolyze the ester bond between ferulic acid and polysaccharides in the plant cell wall. Therefore, the combination of ferulic acid esterase with non-starch polysaccharide enzymes such as cellulase and alkaline protease can theoretically improve the decomposition efficiency of cellulose and help release the main active ingredients in the white willow bark. In addition, the present invention uses microwave-assisted enzymatic hydrolysis, which directly heats the material through molecular polarization or ion conductivity effect, quickly and evenly raises the temperature, significantly improves the enzymatic hydrolysis efficiency and thereby increases the release rate of active substances in the white willow bark, and has the advantages of high selectivity, short operation time and high extraction efficiency.

[0032] (3) The preparation method of the antibacterial composition provided by the present invention has a simple operation process and is suitable for industrial production.

[0033] (4) The antibacterial composition provided by the present invention is used to prepare female private parts care products and has good application value. DETAILED DESCRIPTION

[0034] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined purpose of the invention, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0035] In the following examples, experimental methods without specific conditions were generally performed under conventional conditions or those recommended by the manufacturer. Materials and reagents used were commercially available unless otherwise specified.

[0036] Some of the raw materials and their sources are as follows:

[0037] Willow bark powder was purchased from Shanxi Luyang Biotechnology Co., Ltd.;

[0038] Commercially available white willow bark extract was purchased from Guangzhou Snoco Biotechnology Co., Ltd.;

[0039] Melaleuca alternifolia leaf oil was purchased from Guangzhou Xuanyi Flavors & Fragrances Co., Ltd.

[0040] Sandalwood essential oil was purchased from Xiamen Hongyao Trading Co., Ltd.

[0041] Cedar wood oil was purchased from Jiangxi Cuiyuyuan Biotechnology Co., Ltd.

[0042] Fructooligosaccharide was purchased from Anhui Weimao Biotechnology Co., Ltd.

[0043] Inulin was purchased from Anhui Weimao Biotechnology Co., Ltd.

[0044] Eucalyptus essential oil was purchased from Xiamen Hongyao Trading Co., Ltd.

[0045] Cellulase was purchased from Shanghai Yuanye Biotechnology Co., Ltd. with an enzyme activity of 50 U / mg;

[0046] Feruloyl esterase was purchased from Guangdong Wengjiang Chemical Reagent Co., Ltd. with an enzyme activity of 500 U / mg;

[0047] Alkaline protease was purchased from Shanghai Kanglang Biotechnology Co., Ltd. with an enzyme activity of 200 U / mg.

[0048] The weight percentages of the components in the antibacterial and anti-itching compositions of the examples and comparative examples of the present invention are shown in Table 1 below:

[0049] Table 1 Parts by weight of each component in the antibacterial composition

[0050]

[0051]

[0052] Note: “-” represents that the corresponding component is not added, and the missing amount is proportionally distributed to the remaining components; and the total mass of compositions 1-3 and compositions ①-⑩ are the same.

[0053] The preparation methods of the white willow bark extracts in the antibacterial composition 1 and antibacterial compositions ②-④ are as follows:

[0054] The preparation method of the white willow bark extract comprises the following steps:

[0055] S1. Taking willow bark powder, and mixing the willow bark powder and distilled water in a certain proportion to obtain a mixed solution I;

[0056] S2, adding a complex enzyme to the mixed solution I, performing microwave treatment, and then performing constant temperature enzymolysis treatment to obtain an enzymolysis solution;

[0057] S3, inactivating the enzyme, filtering, and concentrating under reduced pressure on the enzymatic hydrolyzate to obtain a willow bark extract;

[0058] The complex enzyme consists of cellulase, ferulic acid esterase and alkaline protease in a mass ratio of 2.5:0.7:0.5;

[0059] In step S1, the ratio of willow bark powder to distilled water is 1 g:15 mL;

[0060] The total amount of the complex enzyme added in step S2 is 2% of the mass of the white willow bark;

[0061] The microwave power in step S2 is 350W and the microwave time is 15 minutes;

[0062] The enzymatic hydrolysis temperature in step S2 is 55° C., the enzymatic hydrolysis pH is 5.5, and the enzymatic hydrolysis time is 1.5 h;

[0063] The enzyme inactivation temperature in step S3 is 80°C and the enzyme inactivation time is 15 minutes;

[0064] The step S3 adopts high-speed centrifugal filtration, the centrifugal speed is 8000r / min, and the centrifugal time is 20min;

[0065] The reduced pressure concentration in step S3 is to concentrate under reduced pressure to 30% of the original volume at a pressure of 0.07 MPa;

[0066] The preparation method of the antibacterial composition 1 comprises the following steps:

[0067] 1) mixing willow bark extract, oligofructose, inulin, and water, stirring and heating to 55° C. until completely dissolved to obtain mixture I;

[0068] 2) mixing Melaleuca alternifolia leaf oil, sandalwood essential oil, cedarwood oil, and an emulsifier to obtain a mixture II;

[0069] 3) Mix mixture I and mixture II, stir and heat to 45°C, and mix well to obtain the product;

[0070] In step 1), the weight ratio of water to the sum of the weight of oligofructose and inulin is 1:1;

[0071] The emulsifier is PPG-26-butane ether-26, and the added amount of the emulsifier is 7% of the total mass of the Melaleuca alternifolia leaf oil, the sandalwood essential oil and the cedar wood oil.

[0072] The preparation method of the antibacterial composition 2 and its component white willow bark extract is as follows:

[0073] The preparation method of the white willow bark extract comprises the following steps:

[0074] S1. Taking willow bark powder, and mixing the willow bark powder and distilled water in a certain proportion to obtain a mixed solution I;

[0075] S2, adding a complex enzyme to the mixed solution I, performing microwave treatment, and then performing constant temperature enzymolysis treatment to obtain an enzymolysis solution;

[0076] S3, inactivating the enzyme, filtering, and concentrating under reduced pressure on the enzymatic hydrolyzate to obtain a willow bark extract;

[0077] The complex enzyme consists of cellulase, ferulic acid esterase and alkaline protease in a mass ratio of 3:0.5:0.5;

[0078] In step S1, the ratio of willow bark powder to distilled water is 1 g:10 mL;

[0079] The total amount of the complex enzyme added in step S2 is 1.5% of the mass of the white willow bark;

[0080] The microwave power in step S2 is 300W and the microwave time is 20min;

[0081] The enzymatic hydrolysis temperature in step S2 is 60° C., the enzymatic hydrolysis pH is 6.0, and the enzymatic hydrolysis time is 2 h;

[0082] The enzyme inactivation temperature in step S3 is 75°C and the enzyme inactivation time is 10 minutes;

[0083] The step S3 adopts high-speed centrifugal filtration, the centrifugal speed is 8000r / min, and the centrifugal time is 20min;

[0084] The reduced pressure concentration in step S3 is to concentrate under reduced pressure to 30% of the original volume at a pressure of 0.07 MPa;

[0085] The preparation method of the antibacterial composition 2 comprises the following steps:

[0086] 1) mixing willow bark extract, oligofructose, inulin, and water, stirring and heating to 50° C. until completely dissolved to obtain mixture I;

[0087] 2) mixing Melaleuca alternifolia leaf oil, sandalwood essential oil, cedarwood oil, and an emulsifier to obtain a mixture II;

[0088] 3) Mix mixture I and mixture II, stir and heat to 50°C, and mix until uniform;

[0089] In step 1), the weight ratio of water to the sum of the weight of oligofructose and inulin is 1:1;

[0090] The emulsifier is PEG-40 hydrogenated castor oil, and the added amount of the emulsifier is 10% of the total mass of the Melaleuca alternifolia leaf oil, the sandalwood essential oil and the cedarwood oil.

[0091] The preparation method of the antibacterial composition 3 and its component white willow bark extract is as follows:

[0092] The preparation method of the white willow bark extract comprises the following steps:

[0093] S1. Taking willow bark powder, and mixing the willow bark powder and distilled water in a certain proportion to obtain a mixed solution I;

[0094] S2, adding a complex enzyme to the mixed solution I, performing microwave treatment, and then performing constant temperature enzymolysis treatment to obtain an enzymolysis solution;

[0095] S3, inactivating the enzyme, filtering, and concentrating under reduced pressure on the enzymatic hydrolyzate to obtain a willow bark extract;

[0096] The complex enzyme consists of cellulase, ferulic acid esterase and alkaline protease in a mass ratio of 2:1:0.5;

[0097] In step S1, the ratio of willow bark powder to distilled water is 1 g: 20 mL;

[0098] The total amount of the complex enzyme added in step S2 is 2.5% of the mass of the white willow bark;

[0099] The microwave power in step S2 is 400W and the microwave time is 10 minutes;

[0100] The enzymatic hydrolysis temperature in step S2 is 50° C., the enzymatic hydrolysis pH is 5.0, and the enzymatic hydrolysis time is 1 h;

[0101] The enzyme inactivation temperature in step S3 is 85°C and the enzyme inactivation time is 20 minutes;

[0102] The step S3 adopts high-speed centrifugal filtration, the centrifugal speed is 8000r / min, and the centrifugal time is 20min;

[0103] The reduced pressure concentration in step S3 is to concentrate under reduced pressure to 30% of the original volume at a pressure of 0.07 MPa;

[0104] The preparation method of the antibacterial composition 3 comprises the following steps:

[0105] 1) mixing willow bark extract, oligofructose, inulin, and water, stirring and heating to 60° C. until completely dissolved to obtain mixture I;

[0106] 2) mixing Melaleuca alternifolia leaf oil, sandalwood essential oil, cedarwood oil, and an emulsifier to obtain a mixture II;

[0107] 3) Mix mixture I and mixture II, stir and heat to 40°C, and mix until uniform;

[0108] In step 1), the weight ratio of water to the sum of the weight of oligofructose and inulin is 1:1;

[0109] The emulsifier is lecithin, and the added amount of the emulsifier is 5% of the total mass of the Melaleuca alternifolia leaf oil, the sandalwood essential oil and the cedarwood oil.

[0110] The preparation method of the above-mentioned antibacterial composition ① comprises the following steps:

[0111] 1) Mixing oligofructose, inulin, and water, stirring and heating to 55° C., and dissolving completely to obtain mixture I;

[0112] 2) mixing Melaleuca alternifolia leaf oil, sandalwood essential oil, cedarwood oil, and an emulsifier to obtain a mixture II;

[0113] 3) Mix mixture I and mixture II, stir and heat to 45°C, and mix well to obtain the product;

[0114] In step 1), the weight ratio of water to the sum of the weight of oligofructose and inulin is 1:1;

[0115] The emulsifier is PPG-26-butane ether-26, and the added amount of the emulsifier is 7% of the total mass of the Melaleuca alternifolia leaf oil, the sandalwood essential oil and the cedar wood oil.

[0116] The preparation method of the above-mentioned antibacterial composition ② comprises the following steps:

[0117] 1) mixing willow bark extract, oligofructose, inulin, and water, stirring and heating to 55° C. until completely dissolved to obtain mixture I;

[0118] 2) uniformly mixing the sandalwood essential oil, cedarwood oil, and emulsifier to obtain a mixture II;

[0119] 3) Mix mixture I and mixture II, stir and heat to 45°C, and mix well to obtain the product;

[0120] In step 1), the weight ratio of water to the sum of the weight of oligofructose and inulin is 1:1;

[0121] The emulsifier is PPG-26-butanol polyether-26, and the added amount of the emulsifier is 7% of the total mass of the sandalwood essential oil and the cedar wood oil.

[0122] The preparation method of the above-mentioned antibacterial composition ③ comprises the following steps:

[0123] 1) mixing willow bark extract, oligofructose, inulin, and water, stirring and heating to 55° C. until completely dissolved to obtain mixture I;

[0124] 2) mixing the Melaleuca alternifolia leaf oil, cedar wood oil, and emulsifier to obtain a mixture II;

[0125] 3) Mix mixture I and mixture II, stir and heat to 45°C, and mix well to obtain the product;

[0126] In step 1), the weight ratio of water to the sum of the weight of oligofructose and inulin is 1:1;

[0127] The emulsifier is PPG-26-butane ether-26, and the added amount of the emulsifier is 7% of the total mass of the Melaleuca alternifolia leaf oil and the cedar wood oil.

[0128] The preparation method of the above-mentioned antibacterial composition ④ comprises the following steps:

[0129] 1) mixing willow bark extract, oligofructose, inulin, and water, stirring and heating to 55° C. until completely dissolved to obtain mixture I;

[0130] 2) uniformly mixing the Melaleuca alternifolia leaf oil, sandalwood essential oil, and an emulsifier to obtain a mixture II;

[0131] 3) Mix mixture I and mixture II, stir and heat to 45°C, and mix well to obtain the product;

[0132] In step 1), the weight ratio of water to the sum of the weight of oligofructose and inulin is 1:1;

[0133] The emulsifier is PPG-26-butane ether-26, and the added amount of the emulsifier is 7% of the total mass of the Melaleuca alternifolia leaf oil and the sandalwood essential oil.

[0134] The only difference between the antibacterial composition ⑤ and the antibacterial composition 1 is that the white willow bark extract in the antibacterial composition ⑤ is a commercially available product, and other conditions are the same as those of the antibacterial composition 1.

[0135] The antibacterial composition ⑥ differs from the antibacterial composition 1 only in that the Melaleuca alternifolia leaf oil in the antibacterial composition ⑥ is replaced with eucalyptus essential oil, and other conditions are the same as those of the antibacterial composition 1.

[0136] Compared with the antibacterial composition 1, the above-mentioned antibacterial composition ⑦ differs only in that in the preparation method of the white willow bark extract of the antibacterial composition ⑦, the mass ratio of cellulase, ferulic acid esterase and alkaline protease is 1.5:1.5:0.5, and the total addition amount of the complex enzyme remains unchanged. Other conditions are consistent with those of the antibacterial composition 1.

[0137] The above-mentioned antibacterial composition ⑧ is different from the antibacterial composition 1 only in that in the preparation method of the white willow bark extract of the antibacterial composition ⑧, the mass ratio of cellulase, ferulic acid esterase and alkaline protease is 1:0.7:2, and the total addition amount of the complex enzyme remains unchanged. The other conditions are the same as those of the antibacterial composition 1.

[0138] The difference between the above antibacterial composition 9 and the antibacterial composition 1 is that in the preparation method of the white willow bark extract of the antibacterial composition 9, ferulic acid esterase is replaced by hemicellulase, and other conditions are the same as those of the antibacterial composition 1.

[0139] Compared with the antibacterial composition 1, the only difference between the above-mentioned antibacterial composition ⑩ is that the weight proportions of the white willow bark extract, Melaleuca alternifolia leaf oil, sandalwood essential oil and cedar wood oil in the antibacterial composition ⑩ are 22 parts, 6 parts, 8 parts and 15 parts respectively, and the other conditions are the same as those of the antibacterial composition 1.

[0140] Performance test 1 antibacterial performance test

[0141] This test refers to the test method in Appendix C4 of the Hygiene Standard for Disposable Sanitary Products GB15979-2002.

[0142] Preparation of bacterial suspension: Before the experiment, Staphylococcus aureus, Escherichia coli and Candida albicans were streaked onto agar slants and cultured at 36.0°C for 24 h. The cultured test bacteria slants were washed with 0.03 mol / L PBS to prepare a suspension with a viable count of 8 × 10 4 CFU / mL of bacterial suspension.

[0143] Detection method: The test liquids were respectively the test groups (antibacterial composition groups 1-3 and antibacterial composition groups ①-⑩), and the blank control sample solution was PBS. Prepare 3 portions for each group, and place 5.0 mL of sample solution in each tube into sterile test tubes. Take the above bacterial suspension, add 100 μL to 5.0 mL of the test liquid and the control sample solution, mix evenly, start the timer, and let it act for 2 minutes. Pipette 0.5 mL of the sample solution into a test tube containing 5.0 mL of PBS, mix thoroughly, dilute, and then take 10 -1 , 10 -2 For these two dilutions, 0.5 mL was respectively aspirated and placed on two plates. The plates were poured with culture medium cooled to 45°C (nutrient agar medium was used for the plates with Staphylococcus aureus or Escherichia coli, and Sabouraud agar medium was used for the plates with Candida albicans). The plates were rotated to make them fully uniform. After the agar solidified, the plates were turned over. The plates with Staphylococcus aureus or Escherichia coli were cultured at 36.0°C for 48 h, and the plates with Candida albicans were cultured at 28.0°C for 72 h. The viable colonies were counted.

[0144] The antibacterial rate was calculated according to formula 1): X = (AB) / A × 100%;

[0145] In formula 1), X is the inhibition rate (%); A is the average colony count of the blank control solution; and B is the average colony count of the test solution.

[0146] Evaluation criteria: If the antibacterial rate is ≥50% to 90%, the product has antibacterial effect; if the antibacterial rate is ≥90%, the product has a strong antibacterial effect.

[0147] The test results are shown in Table 2.

[0148] Table 2 Antibacterial performance test results

[0149]

[0150]

[0151] The test results are shown in Table 2. The antibacterial rates of antibacterial compositions 1-3 against Staphylococcus aureus, Escherichia coli and Candida albicans were all as high as 99.99%, indicating excellent antibacterial effects. The antibacterial rates of antibacterial compositions ①-⑩ against Staphylococcus aureus, Escherichia coli and Candida albicans were lower than those of antibacterial compositions 1-3, indicating that the components and proportions of the white willow bark extract, Melaleuca alternifolia leaf oil, sandalwood essential oil and cedar wood oil in the composition, as well as the type and proportion range of the complex enzyme used in the preparation process of the white willow bark extract, will affect the antibacterial effect of the antibacterial composition.

[0152] Specifically, 1) the antibacterial composition groups ①-④ did not add one of the components of white willow bark extract, Melaleuca alternifolia leaf oil, sandalwood essential oil and cedar wood oil, respectively, and the antibacterial composition group ⑥ used eucalyptus essential oil instead of Melaleuca alternifolia leaf oil, and the antibacterial effects of the antibacterial compositions ①-④ and ⑥ were significantly worse than those of the antibacterial composition group 1, indicating that there was a synergistic effect between the white willow bark extract, Melaleuca alternifolia leaf oil, sandalwood essential oil and cedar wood oil, and none of the four was indispensable in the composition. Further, the weight ratios of the white willow bark extract, Melaleuca alternifolia leaf oil, sandalwood essential oil and cedar wood oil in the antibacterial composition group ⑩ were different, and the antibacterial effect of the antibacterial composition ⑩ was worse than that of the antibacterial composition group 1, indicating that even if the components are the same, the different ratios of the components in the composition also have a certain effect on its antibacterial effect. 2) The antibacterial composition of group ⑤ used a commercially available white willow bark extract, and its antibacterial effect was significantly worse than that of antibacterial compositions 1 and ⑦-9, indicating that the microwave-assisted enzymatic hydrolysis step used in this application facilitates the release of the active ingredients in the white willow bark extract, and that the white willow bark extract prepared by the preparation method of the present invention has a better antibacterial effect. Furthermore, in the preparation method of the white willow bark extract involved in antibacterial composition group 9, feruloyl esterase was replaced with hemicellulase, and its antibacterial effect was significantly worse than that of antibacterial composition 1, indicating that the type of enzyme in the complex enzyme directly affects the enzymatic hydrolysis effect, and the combination of cellulase, feruloyl esterase, and alkaline protease has the best effect. The combined use of the three can significantly improve the enzymatic hydrolysis efficiency of white willow bark, thereby promoting the release of the active ingredients in white willow bark. In addition, in the preparation method of the white willow bark extract involved in the antibacterial composition group ⑦-⑧, the same type of complex enzyme was used for enzymatic hydrolysis as in the antibacterial composition group 1, the only difference being the different ratios of the enzymes. The antibacterial effects of the antibacterial compositions of the groups ⑦-⑧ were also worse than that of the antibacterial composition group 1, indicating that even with the same combination of complex enzymes, different ratios will affect the enzymatic hydrolysis effect to a certain extent, and thus affect the antibacterial effect of the antibacterial composition.

[0153] In summary, the present invention not only optimizes the preparation method of the white willow bark extract, that is, the white willow bark extract is prepared by composite enzymatic hydrolysis of the white willow bark with cellulase, ferulic acid esterase and alkaline protease, which can significantly improve the enzymatic hydrolysis efficiency and thus promote the release of active ingredients in the white willow bark; but also achieves a synergistic effect between the white willow bark extract, Melaleuca alternifolia leaf oil, sandalwood essential oil and cedar wood oil, so that the prepared antibacterial composition has a significant antibacterial effect, which can solve the problem that the existing female private antibacterial agents have limited antibacterial effects and long-term use will aggravate gynecological diseases.

[0154] Performance test 2 Vaginal mucosal irritation test

[0155] This test refers to the vaginal mucosal irritation test method in 2.3.5 of the 2002 edition of the "Technical Specifications for Disinfection" to detect the irritation effect and intensity of the antibacterial composition prepared in the present invention on the vaginal mucosa of experimental animals.

[0156] Experimental animals: Female New Zealand rabbits, weighing 2.0-2.5 kg. Before the experiment, rabbits were examined for vaginal discharge, congestion, edema, and other lesions. Rabbits were fed a standard diet and housed individually in a pathogen-free environment at a constant temperature of 24-28°C and a relative humidity of 50%-60%.

[0157] Detection method: Experimental animals were randomly divided into an exposure group (antibacterial composition groups 1-3) and a blank control group. The test solution of the exposure group was the antibacterial composition prepared by antibacterial composition groups 1-3 respectively, and the test solution of the blank control group was normal saline with a concentration of 0.9%. For each group of 3 animals, a blunt-tipped hose with a length of 8 cm was connected to a 2 ml syringe. The syringe and catheter were filled with the test solution and used for standby use. One set was prepared for each animal. The animal was fixed on its back, and the perineum and vaginal opening were exposed. The catheter was soaked with the test solution and gently inserted into the vagina 5 cm. 2 ml of the test solution was slowly injected with a syringe. The catheter was withdrawn to complete the exposure. The exposure was repeated every 24 hours for 5 consecutive days. The experimental animals in the control group were treated with normal saline in the same way. 24 hours after the last exposure, the animals were killed by gas embolism, the intact vagina was removed by laparotomy, and longitudinal incision was made. The naked eye was observed to see if there were any signs of congestion, edema, etc. for reference when taking pathological samples. The vagina was then placed in a 10% formalin solution and fixed for 24 hours. Tissues from the two ends and three central parts of the vagina were selected for preparation and histopathological examination was performed after HE staining. The irritation response of the vaginal mucosa was scored according to the provisions of Table 3. The irritation response scores of the three parts of the three animals in each group were added together and then divided by the total number of observations (number of animals × 3) to obtain the average score of the vaginal mucosal irritation response in each group. The irritation index was obtained by subtracting the average score of the control group from the average score of the poisoned group, and the irritation intensity was graded according to Table 4.

[0158] Test results: The test results are shown in Table 5. The vaginal mucosal irritation reaction intensity of animals in the blank control group and the poisoned group was none, indicating that the antibacterial composition prepared by the present invention is mild and non-irritating.

[0159] Table 3 Vaginal mucosal irritation response scoring criteria

[0160]

[0161]

[0162] Note: Stimulus response integral = A+B+C+D.

[0163] Table 4 Vaginal mucosal irritation intensity grading standard

[0164]

[0165] Note: Stimulation index = average score of experimental group - average score of blank control group.

[0166] Table 5 Vaginal mucosal irritation intensity grading results

[0167]

[0168]

[0169] The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as above in terms of a preferred embodiment, it is not intended to limit the present invention. Any person skilled in the art can, without departing from the scope of the technical solution of the present invention, make some changes or modifications to equivalent embodiments using the technical contents disclosed above. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A female private part antibacterial composition, characterized in that: The antibacterial composition comprises the following components by weight: 12-20 parts of white willow bark extract, 8-16 parts of Melaleuca alternifolia leaf oil, 9-15 parts of sandalwood essential oil, and 8-14 parts of cedar wood oil; and the preparation method of the white willow bark extract comprises the following steps: S1. Taking willow bark powder, and mixing the willow bark powder and distilled water in a certain proportion to obtain a mixed solution I; S2, adding a complex enzyme to the mixed solution I, performing microwave treatment, and then performing constant temperature enzymolysis treatment to obtain an enzymolysis solution; S3, inactivating the enzyme, filtering, and concentrating under reduced pressure on the enzymatic hydrolyzate to obtain a willow bark extract; The complex enzyme consists of cellulase, ferulic acid esterase and alkaline protease in a mass ratio of 2-3:0.5-1:0.

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2. The antibacterial composition according to claim 1, characterized in that The antibacterial composition further comprises the following components in parts by weight: 2-6 parts of oligofructose and 1-5 parts of inulin.

3. The antibacterial composition according to claim 1, characterized in that The antibacterial composition comprises the following components in parts by weight: 16 parts of white willow bark extract, 12 parts of Melaleuca alternifolia leaf oil, 12 parts of sandalwood essential oil, and 11 parts of cedar wood oil.

4. The antibacterial composition according to any one of claims 1 to 3, characterized in that In step S1, the ratio of the willow bark powder to distilled water is 1 g: 10-20 mL.

5. The antibacterial composition according to any one of claims 1 to 3, characterized in that The total amount of the complex enzyme added in step S2 is 1.5-2.5% of the mass of the white willow bark, and the complex enzyme is composed of cellulase, ferulic acid esterase and alkaline protease in a mass ratio of 2.5:0.7:0.

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6. The antibacterial composition according to any one of claims 1 to 3, characterized in that The microwave power in step S2 is 300-400W, and the microwave time is 10-20 minutes.

7. The antibacterial composition according to any one of claims 1 to 3, characterized in that The enzymatic hydrolysis temperature in step S2 is 50-60° C., the enzymatic hydrolysis pH is 5.0-6.0, and the enzymatic hydrolysis time is 1-2 h.

8. The antibacterial composition according to any one of claims 1 to 3, characterized in that The enzyme inactivation temperature in step S3 is 75-85° C., and the enzyme inactivation time is 10-20 min.

9. A method for preparing the antibacterial composition according to any one of claims 1 to 8, characterized in that: The following steps are involved: 1) mixing willow bark extract, oligofructose, inulin, and water, stirring and heating to 50-60° C. until completely dissolved to obtain mixture I; 2) mixing Melaleuca alternifolia leaf oil, sandalwood essential oil, cedarwood oil, and an emulsifier to obtain a mixture II; 3) Mix mixture I and mixture II, stir and heat to 40-50°C, and mix well to obtain the product.

10. Use of the antibacterial composition according to any one of claims 1 to 8 in the preparation of female private parts care products.

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