Pharmaceutical composition for treating chilblain by combining golden larch bark and shrubalthea bark and application of pharmaceutical composition

Abstract

The invention discloses a golden larch bark and shrubalthea bark combined medicine composition for treating chilblain and application thereof, and relates to the technical field of medicine, and the medicine composition comprises golden larch bark extract and shrubalthea bark extract. The invention also discloses an application of the pharmaceutical composition in preparation of chilblain treatment drugs. The invention further discloses black ointment for treating chilblain. The black ointment comprises the pharmaceutical composition. The golden larch bark and the shrubalthea bark are combined for chilblain treatment for the first time, the synergistic effect of the golden larch bark and the shrubalthea bark is good, skin chilblain can be rapidly treated, skin tissue can be improved, and meanwhile the effects of promoting blood circulation, inhibiting inflammation and resisting bacteria are achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of medicine, and in particular to a pharmaceutical composition for treating frostbite by combining thorn bark and hibiscus bark and an application thereof. Background Art

[0002] Chilblains are a localized skin disease caused by spasmodic contraction of skin blood vessels due to cold exposure, leading to microcirculatory disorders, tissue ischemia and hypoxia, and inflammatory reactions. Clinical manifestations include erythema, swelling, and itching, which can develop into blisters and ulcers in severe cases. The main treatment methods currently include: topical medications, such as polysulfated mucopolysaccharide cream (which promotes blood circulation through heparin analogs) and glucocorticoids (which inhibit inflammation), but long-term use can easily cause side effects such as skin atrophy and pigmentation; physical therapy, such as infrared irradiation and local hot compresses, but the efficacy is unstable and relies on equipment support.

[0003] Traditional frostbite treatment drugs have limited efficacy, and long-term use of glucocorticoids can easily lead to skin barrier damage. Therefore, there is an urgent need to find a new frostbite treatment drug. Summary of the Invention

[0004] In order to solve the above technical problems, the purpose of the present invention is to provide a pharmaceutical composition for treating chilblains by combining the bark of thorny stem and the bark of hibiscus.

[0005] The technical solution of the present invention to solve the above technical problems is as follows: providing a pharmaceutical composition for treating frostbite by combining schizonepeta serrata and hibiscus bark, comprising schizonepeta serrata extract and hibiscus bark extract.

[0006] Furthermore, the extract of the Chinese thorn bark and the extract of the hibiscus bark are extracted using sesame oil, and the mass ratio of the raw materials of the Chinese thorn bark and the hibiscus bark is 1:0.5-1.5.

[0007] The present invention also provides the use of the above-mentioned pharmaceutical composition in the preparation of frostbite treatment drugs

[0008] The present invention also provides a black ointment for treating frostbite, comprising the above-mentioned pharmaceutical composition.

[0009] Furthermore, a method for preparing black ointment for treating frostbite comprises the following steps:

[0010] (1) mixing the bark of thorny vine and the bark of hibiscus mume, adding sesame oil and soaking it to obtain a raw material mixture;

[0011] (2) heating the raw material mixture obtained in step (1) to 110-130° C. until bubbling occurs, then heating to 190-210° C. until the surface of the medicinal material is charred, cooling, and filtering to obtain a mixed extract;

[0012] (3) adding yellow lead to the mixed extract obtained in step (2), stirring and heating at 140-160° C. until yellow lead reacts with sesame oil, releasing irritating gas and the surface turns black, then pouring into water and stirring, then discarding the water, and heating again under stirring until it becomes sticky, and finally cooling to form a black ointment.

[0013] Furthermore, in step (1), the mass ratio of the bark of the Chinese thorn tree and the bark of the hibiscus tree is 1:0.5-1.5.

[0014] Furthermore, in step (1), the ratio of the total mass of the schisandra chinensis bark and the hibiscus bark to the sesame oil is 1 g: 3-7 mL. Preferably, the ratio of the total mass of the schisandra chinensis bark and the hibiscus bark to the sesame oil is 1 g: 5 mL.

[0015] Furthermore, in step (1), soak for 20-30 hours.

[0016] Furthermore, in step (2), the mixture is filtered through a 30-50 mesh sieve.

[0017] Furthermore, in step (2), the mass volume ratio of the mixed extract and yellow lead is 8-12 mL: 1 g.

[0018] The present invention has the following beneficial effects:

[0019] The present invention combines the bark of thorny vine and the bark of hibiscus for the treatment of frostbite for the first time. The two have a good synergistic effect, can quickly treat skin frostbite and improve skin tissue, and have the effects of promoting blood circulation, inhibiting inflammation and antibacterial. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 Schematic diagram of the rat frostbite model;

[0021] Figure 2 This is a schematic diagram of drug application for frostbite model;

[0022] Figure 3 This is a surface image of the skin of a model mouse treated with black ointment in Example 2;

[0023] Figure 4 It is a line graph of IL-6 secretion in the serum of model mice;

[0024] Figure 5 It is a line graph of MCP-1 secretion in the serum of model mice;

[0025] Figure 6 This is the HE staining observation picture of the skin tissue of the control group in Experimental Example 1;

[0026] Figure 7 This is the HE staining observation picture of the skin tissue of the experimental group in Experimental Example 1. DETAILED DESCRIPTION

[0027] The principles and features of the present invention are described below. The examples are intended only to illustrate the present invention and are not intended to limit the scope of the present invention. In the examples, where specific conditions are not specified, conventional conditions or manufacturer-recommended conditions were used. Reagents or instruments used where the manufacturer is not specified are conventional products that can be purchased commercially.

[0028] Example 1

[0029] A pharmaceutical composition for treating frostbite by combining thorn bark and hibiscus bark, comprising a thorn bark extract and a hibiscus bark extract; wherein the thorn bark extract and the hibiscus bark extract are extracted with sesame oil, and the mass ratio of the raw materials thorn bark to hibiscus bark is 1:1.

[0030] Example 2

[0031] A black ointment for treating frostbite, the preparation method of which comprises the following steps:

[0032] (1) Mix the bark of the Chinese thorn tree and the bark of the hibiscus tree in a mass ratio of 1:1, add sesame oil and soak for 24 hours to obtain a raw material mixture;

[0033] (2) heating the raw material mixture obtained in step (1) to 120° C. until bubbling occurs, then heating to 200° C. until the surface of the medicinal material is charred, and filtering through a 40-mesh sieve after cooling to obtain a mixed extract;

[0034] (3) Add yellow lead to the mixed extract obtained in step (2) at a mass volume ratio of 10 mL: 1 g, and heat with stirring at 150° C. until yellow lead reacts with sesame oil, releasing irritating gas and the surface turns black. Then pour into water and stir, then discard the water, and heat again under stirring until it becomes sticky, and finally cool to form a black ointment.

[0035] Example 3

[0036] A black ointment for treating frostbite, the preparation method of which comprises the following steps:

[0037] (1) Mix the bark of the Chinese thorn tree and the bark of the hibiscus tree in a mass ratio of 1:0.5, add sesame oil and soak for 20 hours to obtain a raw material mixture;

[0038] (2) heating the raw material mixture obtained in step (1) to 110° C. until bubbling occurs, then heating to 190° C. until the surface of the medicinal material is charred, and filtering through a 30-mesh sieve after cooling to obtain a mixed extract;

[0039] (3) Add yellow lead to the mixed extract obtained in step (2) at a mass volume ratio of 8 mL:1 g, and heat with stirring at 140° C. until yellow lead reacts with sesame oil, releasing irritating gas and the surface turns black. Then pour into water and stir, then discard the water, and heat under stirring until it becomes sticky, and finally cool to form a black ointment.

[0040] Example 4

[0041] A black ointment for treating frostbite, the preparation method of which comprises the following steps:

[0042] (1) Mix the bark of the Chinese thorn tree and the bark of the hibiscus tree in a mass ratio of 1:1.5, add sesame oil and soak for 30 hours to obtain a raw material mixture;

[0043] (2) heating the raw material mixture obtained in step (1) to 130° C. until bubbling occurs, then heating to 210° C. until the surface of the medicinal material is charred, and filtering through a 50-mesh sieve after cooling to obtain a mixed extract;

[0044] (3) Add yellow lead to the mixed extract obtained in step (2) at a mass volume ratio of 12 mL: 1 g, and heat with stirring at 160° C. until yellow lead reacts with sesame oil, releasing irritating gas and the surface turns black. Then pour into water and stir, then discard the water, and heat again under stirring until it becomes sticky, and finally cool to form a black ointment.

[0045] Comparative Example 1

[0046] A black ointment of a thorn bark extract, the preparation method of which comprises the following steps:

[0047] (1) Add the bark of the Chinese thorn tree to sesame oil and soak it for 24 hours to obtain a raw material mixture;

[0048] (2) heating the raw material mixture obtained in step (1) to 120° C. until bubbling occurs, then heating to 200° C. until the surface of the medicinal material is charred, and filtering through a 40-mesh sieve after cooling to obtain a mixed extract;

[0049] (3) Add yellow lead to the mixed extract obtained in step (2) at a mass volume ratio of 10 mL: 1 g, and heat with stirring at 150° C. until yellow lead reacts with sesame oil, releasing irritating gas and the surface turns black. Then pour into water and stir, then discard the water, and heat again under stirring until it becomes sticky, and finally cool to form a black ointment.

[0050] Comparative Example 2

[0051] A black ointment of hibiscus bark extract, the preparation method of which comprises the following steps:

[0052] (1) Add hibiscus bark to sesame oil and soak for 24 hours to obtain a raw material mixture;

[0053] (2) heating the raw material mixture obtained in step (1) to 120° C. until bubbling occurs, then heating to 200° C. until the surface of the medicinal material is charred, and filtering through a 40-mesh sieve after cooling to obtain a mixed extract;

[0054] (3) Add yellow lead to the mixed extract obtained in step (2) at a mass volume ratio of 10 mL: 1 g, and heat with stirring at 150° C. until yellow lead reacts with sesame oil, releasing irritating gas and the surface turns black. Then pour into water and stir, then discard the water, and heat again under stirring until it becomes sticky, and finally cool to form a black ointment.

[0055] Test Example 1

[0056] Establishment of a frostbite model in rats: Rats were randomly divided into four groups, with five rats in each group. A frostbite model was established in all groups. The rats' backs were shaved, and an area approximately the size of a one-yuan coin was cut out. A metal object frozen in liquid nitrogen for 15 minutes was applied to the exposed skin on the rats' backs for 10 seconds, once daily for five consecutive days.

[0057] Method of administration: After successful modeling (frostbite model such as Figure 1 The control group was given physiological saline, and the other three groups were smeared with the black ointment of Example 2, Comparative Example 1 and Comparative Example 2, once a day for 7 consecutive days (drug external application as shown). Figure 2 shown).

[0058] Effect evaluation: The local blood flow of the rat back model was monitored, and the four-level lesion grading method was used to score according to Table 1 based on the recovery effect of the rat back skin.

[0059] Table 1 Rating table

[0060]

[0061] After the experiment, the serum inflammatory factor IL-6 and MCP-1 secretion were measured, and the mice were killed by cervical dislocation. The skin tissue from the frostbite area on the back was taken, fixed with 4% paraformaldehyde, embedded in paraffin, and sliced. Then, HE staining was performed and the pathological changes of the skin tissue were observed under a microscope. Experimental results:

[0062] (1) The scoring results of each group are shown in Table 2.

[0063] Table 2 Scoring results

[0064]

[0065]

[0066] The skin surface of the model mice treated with black ointment in Example 2 is as follows: Figure 3 As shown in Table 2, the skin of the model mice was well improved. And as shown in Table 2, compared with the model control group and other drug groups, the back swelling of the rats in the experimental group using the black ointment in Example 2 was reduced, the score was lowered, and the skin pathological damage was improved.

[0067] (2) The secretion of inflammatory factor IL-6 in serum Figure 4 As shown in the results, the IL-6 secretion in different treatment groups showed different changing trends with the progress of the "pre-modeling-drug administration-healing" stage. In the healing period, the IL-6 secretion in the schizonepeta tenuifolia + hibiscus bark group showed a downward trend compared with the control group, indicating that the experimental group using the black ointment in Example 2 (mixed medication) had a significant anti-inflammatory effect.

[0068] (4) MCP-1 secretion in serum Figure 5 As shown, the results showed that the secretion of MCP-1 in the experimental group using the black ointment of Example 2 decreased significantly during the healing period, which indicates that the combination of the black ointment of Example 2, the bark of Vitex chinensis and the bark of Hibiscus mume, has a significant anti-inflammatory effect.

[0069] (4) The results of HE staining of skin tissues in the control group and experimental group are shown in the figure below. Figure 6 and 7 As shown, the results show that the skin tissue is significantly improved after external administration of the black ointment in Example 2.

[0070] Test Example 2

[0071] LPS-induced RAW264.7 macrophages produced TNF-α, and then the black ointment of Example 2, Comparative Example 1 and Comparative Example 2 were added respectively to detect the TNF-α inhibition rate. The results showed that the inhibition rate reached 85.2%, while the inhibition rate of single wormwood bark was only 62.3% and the inhibition rate of single hibiscus bark was only 58.7%, indicating that the combination has a good inflammation inhibition effect.

[0072] Test Example 3

[0073] The results of the inhibition zone test showed that the diameter of the inhibition zone of the black ointment in Example 2 against Staphylococcus aureus reached 18 mm, indicating that it had a good antibacterial effect.

[0074] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent replacements or improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A pharmaceutical composition for treating frostbite by combining wormwood bark and hibiscus bark, characterized in that: Includes chaste tree bark extract and hibiscus bark extract.

2. The pharmaceutical composition according to claim 1, wherein The schisandra chinensis bark extract and the hibiscus bark extract are extracted using sesame oil, and the mass ratio of the raw materials schisandra chinensis bark to the hibiscus bark is 1:0.5-1.

5.

3. Use of the pharmaceutical composition according to claim 1 in preparing a medicament for treating frostbite.

4. A black ointment for treating frostbite, characterized in that: Comprising the pharmaceutical composition according to claim 1.

5. The black ointment for treating frostbite according to claim 4, characterized in that: The preparation method thereof comprises the following steps: (1) mixing the bark of thorny vine and the bark of hibiscus mume, adding sesame oil and soaking it to obtain a raw material mixture; (2) heating the raw material mixture obtained in step (1) to 110-130° C. until bubbling occurs, then heating to 190-210° C. until the surface of the medicinal material is charred, cooling, and filtering to obtain a mixed extract; (3) adding yellow lead to the mixed extract obtained in step (2), stirring and heating at 140-160° C. until yellow lead reacts with sesame oil, releasing irritating gas and the surface turns black, then pouring into water and stirring, then discarding the water, and heating again under stirring until it becomes sticky, and finally cooling to form a black ointment.

6. The black ointment for treating frostbite according to claim 5, characterized in that: In step (1), the mass ratio of the bark of the Chinese thorn tree to the bark of the hibiscus tree is 1:0.5-1.

5.

7. The black ointment for treating frostbite according to claim 5, characterized in that: In step (1), the ratio of the total mass of the bark of the Chinese thorn tree and the bark of the hibiscus tree to the sesame oil is 1 g: 3-7 mL.

8. The black ointment for treating frostbite according to claim 5, characterized in that: In step (1), soak for 20-30 hours.

9. The black ointment for treating frostbite according to claim 5, characterized in that: In step (2), filter with a 30-50 mesh sieve.

10. The black ointment for treating frostbite according to claim 5, characterized in that: In step (2), the mass volume ratio of the mixed extract and yellow lead is 8-12 mL: 1 g.

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