Composite nursing dressing based on animal tissue, deep eutectic solvent and bamboo leaf powder

Abstract

The invention discloses a composite nursing dressing based on animal tissues, a deep-eutectic solvent and bamboo leaf powder and a preparation method of the composite nursing dressing. The animal tissues comprise large intestines, stomachs or aortic arches rich in elastic fibers, and the eutectic solvent is composed of hydrogen bond receptor choline chloride and polymerizable hydrogen bond donors. According to the invention, cheap animal tissues are used as a dressing matrix, bamboo leaf powder rich in plant active ingredients is used as a functional substance, a eutectic solvent is used as a polymeric monomer, an active ingredient dissolving agent and a penetration enhancer, and the composite nursing dressing is prepared by utilizing an ultraviolet irradiation method. The related preparation process is simple, the cost is low, molding is easy, and functional substances are convenient to add. Animal and plant components and the green solvent are integrated, so that good stability is achieved. The obtained composite dressing has good indexes such as flexibility, uniformity, color and luster, mechanical strength and the like, is easy to fit skin, and also has certain air permeability, moisture absorption and moisture retention properties. Active ingredients in the bamboo leaves can play roles in resisting oxidation, preserving moisture, resisting inflammation and relieving skin.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical devices and beauty care materials, and in particular relates to a skin care dressing made of composite components of animal and plant materials with multiple functions and a preparation method thereof. Background Art

[0002] Common livestock farming, such as pigs, cattle, and sheep, produces a large amount of offal after slaughter, commonly known as "offal." Due to dietary restrictions, these offal are generally not processed or consumed as food in foreign countries. However, in China, some of these offal, which are more palatable and nutritious, are consumed as food. With the continuous improvement of living standards, the rapid development of the livestock and related processing industries, and the implementation of circular economy policies, the production of these byproducts has increased dramatically. The need to develop and utilize these offal in a more scientific, efficient, low-cost, and high-value-added manner has become a pressing challenge for many industries. Driven by the rapid development of science and technology, many of these offal and internal organs have become important industrial raw materials, widely used and researched in industries such as biochemistry, food, pharmaceuticals, materials, feed, fertilizers, daily chemicals, and environmental protection. These trends have become the mainstream development trend of animal-derived products and have spawned a number of emerging industries. These products offer advantages such as being naturally derived, having minimal toxicity and side effects, and being easily absorbed by the human body (Meat Science, 2016, 120:54-59). The main challenges currently faced include a lack of diversity in target ingredients and product types (primarily fine chemicals and biological products such as proteins, peptides, and amino acids). Many products also involve complex extraction, separation, and purification processes, resulting in high development costs and long production cycles. It is also worth exploring whether the relevant animal-derived raw materials can be utilized as simply and efficiently as possible. Furthermore, there has been no targeted and focused development of common visceral tissues, tailored to their specific categories and characteristics. For example, the large intestine, stomach, and aortic arch, which are also rich in elastic fiber tissue and smooth muscle cells, are still primarily consumed by the public as food.

[0003] The skin is the largest organ in the body and is also the organ exposed to the complex external environment. It needs to maintain a good healthy state. Therefore, skin care is of great significance for both beauty care and disease treatment. For example, skin ulcers, which are common and frequently occurring surgical diseases, are often caused by physical or chemical factors directly acting on tissues or microbial infection. Small ulcers are easier to heal on their own, but large-area skin ulcers are often characterized by long-term inability to heal, thus affecting the normal function of the body. The relevant tissues will show varying degrees of defects, liquefaction, and even infection and necrosis, accompanied by symptoms such as itching, pain, ulceration, exudation, and even bleeding. They are prone to prolonged or recurring attacks, causing great pain and inconvenience to patients, seriously affecting their work and quality of life. Therefore, it has become one of the long-standing problems in clinical medicine. Common examples include pressure sores (bedsores), a type of pressure ulcer (Surgery (Oxford), 2014, 32(9): 472-476). Due to long-term pressure on local tissues, blood circulation disorders, and persistent ischemia, hypoxia, and nutrient deficiency in local tissues, the skin loses its normal function and can easily cause soft tissue infection and necrosis. Currently, the available large-area skin care dressings are very limited, and research and development innovation is urgently needed. In addition, daily skin care also has a large demand for seasonal sensitivity, after-sun repair, acne removal, redness, moisturizing, whitening, exfoliation / scar removal, and metabolism promotion. The base materials, which are mainly made of non-woven fabrics, plant fibers, and silk fibers, need to be enriched.

[0004] Traditional Chinese medicine already has successful experience in using animal offal for health care. In addition, in recent years, more and more natural products from animal and plant sources have been found to have a range of effects, including anti-ulcer, anti-inflammatory, bacterial growth inhibition, and immunity enhancement. Related preparations and dressings have been well applied in clinical practice and are increasingly valued as a renewable biomass resource. As a representative natural resource, bamboo leaves contain a large amount of phenolic and flavonoid compounds, as well as bioactive polysaccharides and other effective ingredients (Journal of Ethnopharmacology, 2023, 306: 116-166.), such as amino acids, active peptides, and trace elements such as manganese, zinc, and selenium. Its skin care effects mainly include but are not limited to: (1) Antioxidant: It can help scavenge free radicals and slow down skin aging; (2) Moisturizing: It can provide long-lasting moisture and moisturizing effects, making the skin softer and smoother; (3) Anti-inflammatory: It can reduce skin inflammation and discomfort, relieve sensitivity and redness; (4) Soothing: It can soothe the skin, eliminate stress and fatigue, and make the skin healthier and more vibrant; In addition, it has the effects of clearing heat and detoxifying, inhibiting bacteria and antibacterial. With the above multiple effects and low irritation to sensitive skin, it has been gradually used as a characteristic component of some facial masks, lotions, and essences. These characteristics are of great significance for body care in clinical practice, and also provide a scientific basis for the development of more different external dressings.

[0005] Deep eutectic solvent (DES) is a new type of multi-purpose green solvent composed of a hydrogen bond donor (HBD) and a hydrogen bond acceptor (HBA) in a certain molar ratio. It exists in liquid form at room temperature and its application in the pharmaceutical field is currently in its infancy (Proc Natl Acad Sci USA, 2014, 111 (37): 13313-13318.). Due to its flexible and variable composition, unique physical and chemical properties different from conventional media, and good biocompatibility (many HBD and HBA components are directly derived from nature), it has great potential in the application areas of health and functional materials. In particular, when the HBD selected is acrylic acid, maleic acid, itaconic acid, etc., it can be used as a component of the deep eutectic solvent and as a polymerizable monomer; polymerization reaction can occur under conditions such as initiator, ultraviolet light irradiation or heating. The resulting polymerizable deep eutectic solvent (PDES) can be used to prepare high molecular weight polymers, while avoiding the potential safety risks associated with direct use of DES monomers, thereby greatly broadening its application space in the above-mentioned fields.

[0006] Currently, new types of dressings include synthetic fiber dressings, polymer film dressings, foamed polymer dressings, hydrocolloid dressings, alginate dressings, etc.; after the emergence of a large number of dressings based on polymer materials (synthetic fibers), various ingredients derived from nature are increasingly being used to prepare dressing base materials or as addible functional substances. This greatly caters to the current social trend of pursuing a return to nature and the green development and efficient utilization of resources. Overall, its formula and composition can still be further expanded. The strategy of "three-in-one" combining animal and plant raw materials and polymers is worth exploring. At the same time, representative problems such as complex processes, high costs, the need for special equipment, and the system's easy water loss and hardening also need further improvement. For daily skin care products (including cosmetics), advocating natural "environmental beauty" is also a mainstream trend. The excessive presence of chemical raw materials can cause skin thinning, inflammation, sensitivity, etc., and in severe cases, even lead to skin cancer. The present invention provides a composite dressing with a friendly low eutectic solvent, bamboo leaf powder and animal tissue rich in elastic fibers as characteristic components, and a preparation method thereof. The composite dressing has a novel formula, simple preparation, comprehensive functions, and is easy to shape and mass-produce. At the same time, it solves the problem of the material being easily hardened due to water loss, thereby improving the comfort and friendliness of skin contact. It not only achieves the expected basic performance and sustained-release effect of active ingredients, but also promotes the high-value development of inexpensive non-primarily utilized animal and plant resources, providing a useful reference for the preparation of current nursing-type polymer composite materials. Summary of the Invention

[0007] The purpose of the present invention is to provide a multifunctional composite skin care dressing and its preparation method. The characteristic components involved include animal visceral tissue rich in elastic fibers, a deep eutectic solvent, and bamboo leaf powder. The preparation method involved is simple, low-cost, and easy to form without the use of special equipment. The composite film component is stable, has good performance, and has a sustained release effect of active ingredients. During the operation, the animal tissue is first physically prepared into a cell homogenate, and the plant tissue is simultaneously prepared into an ultrafine powder. Then, the entire system including the deep eutectic solvent monomer and the cell homogenate is composited into a transparent to translucent film-like substrate using a photopolymerization method, to which ultrafine powder of plant raw materials capable of releasing active ingredients can be directly added. The good physical and chemical properties of each component in the preparation process reflect obvious combined advantages. The overall properties and basic performance of the animal tissue-deep eutectic solvent-bamboo leaf powder composite dressing are relatively excellent. The deep eutectic solvent is integrated with the animal and plant components, has good stability and biocompatibility, and greatly expands its application in the field of medical materials. The composite dressing exhibits the desired flexibility, uniformity, color, and mechanical strength, easily conforming to the skin and enabling easy cutting. It also possesses adequate breathability, moisture absorption, and moisturizing properties. Furthermore, it can serve as a carrier for the release of active substances. The slowly released bamboo leaf ingredients exert long-lasting antioxidant, moisturizing, anti-inflammatory, and skin-soothing effects. The preparation process involved not only achieves high-value development and comprehensive utilization of low-value "offal" ingredients commonly used in animal processing, but also cleverly integrates animal and plant materials into nursing dressings.

[0008] Technical solution: In order to achieve the above objectives, a composite nursing dressing based on animal tissue-low eutectic solvent-bamboo leaf powder and a preparation method thereof are proposed.

[0009] The composite nursing dressing based on animal tissue-deep eutectic solvent-bamboo leaf powder described in the present invention is characterized in that the dressing matrix used is animal tissue homogenate, including but not limited to large intestine homogenate, stomach homogenate or aortic arch homogenate.

[0010] The above-mentioned composite nursing dressing based on animal tissue-deep eutectic solvent-bamboo leaf powder is characterized in that the deep eutectic solvent used includes but is not limited to choline chloride-acrylic acid (molar ratio of 1:2) or choline chloride-acrylamide (molar ratio of 1:2), which is a polymerization monomer, functional solvent and penetration enhancer.

[0011] The above-mentioned composite nursing dressing based on animal tissue-low eutectic solvent-bamboo leaf powder is characterized in that the bamboo leaf powder used is an addible functional substance obtained by ultrafine grinding of dried bamboo leaves, and the particle size range is 80 to 200 meshes.

[0012] The above-mentioned composite nursing dressing based on animal tissue-deep eutectic solvent-bamboo leaf powder is characterized in that the dressing forming agent used is guar gum.

[0013] The above-mentioned composite nursing dressing based on animal tissue-deep eutectic solvent-bamboo leaf powder is characterized in that the photoinitiator used is 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone or 2-benzyl-2-dimethylamino-1-(4-morpholinophenyl)butanone.

[0014] The above-mentioned composite nursing dressing based on animal tissue-deep eutectic solvent-bamboo leaf powder is characterized by the following specific preparation steps:

[0015] (1) Clean the large intestine, stomach, or aortic arch, wash off the fat, cut into small pieces, and crush them in a 200W tissue homogenizer at a mass ratio of animal tissue: physiological saline = 1:1 for 1 hour; then place the tissue homogenate in a centrifuge at a speed of 5000 r / min and centrifuge for 10 minutes. Discard the supernatant to obtain large intestine homogenate, stomach homogenate, or aortic arch homogenate, respectively.

[0016] (2) animal tissue homogenate: deep eutectic solvent = 1:5 to 2:5 mass ratio is mixed evenly, then guar gum is added according to the mass ratio of guar gum: deep eutectic solvent = 1:20 to 1:10 mass ratio is mixed evenly, and then photoinitiator is added according to the mass ratio of photoinitiator: deep eutectic solvent = 1:50 mass ratio is mixed evenly to form a substrate membrane slurry;

[0017] (3) When a functional substance needs to be added to the substrate, bamboo leaf powder is added at a mass ratio of functional substance to membrane slurry = 1:20, and stirred thoroughly at room temperature to disperse evenly;

[0018] (4) Pour the prepared membrane slurry into a smooth and clean mold, spread it evenly, and control the thickness to be 1-2 mm;

[0019] (5) Irradiate the slurry in the mold under a 6W ultraviolet lamp for 12 to 24 hours. Stop irradiation after the slurry is completely solidified.

[0020] (6) Place the prepared dressing in a drying oven and dry it under reduced pressure at 50°C for 3 hours before taking it out;

[0021] (7) Sterilize the dried dressing by irradiating it with ultraviolet light for 60 minutes;

[0022] (8) Cut the prepared composite nursing dressing into regular shapes, package them, and seal them for storage. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more completely illustrate the technical solutions of the specific embodiments of the present invention, the following briefly describes the drawings required in the embodiments. The drawings described are only part of the embodiments of the present invention. Those skilled in the art should be able to derive other drawings of the embodiments based on these drawings. Among them:

[0024] Figure 1 This is the appearance of the substrate obtained by animal tissue-deep eutectic solvent polymerization in the present invention (without the addition of the functional substance bamboo leaf powder, the animal tissue in a is the large intestine, b is the stomach, and c is the aortic arch).

[0025] Figure 2 This is a tensile test of the substrate (without the addition of the functional substance bamboo leaf powder) obtained based on animal tissue-deep eutectic solvent polymerization in the present invention.

[0026] Figure 3 The figures show the bonding effect of the substrate (without adding the functional substance bamboo leaf powder) obtained by polymerization of animal tissue-deep eutectic solvent in the present invention on different surfaces.

[0027] Figure 4 The present invention shows the appearance of the composite nursing dressing based on animal tissue-deep eutectic solvent-bamboo leaf powder (a) and a scanning electron microscope photograph of its surface (b).

[0028] Figure 5 This is the infrared spectrum of the composite nursing dressing based on animal tissue-deep eutectic solvent-bamboo leaf powder in the present invention.

[0029] Figure 6 This is the tensile stress curve of the composite nursing dressing based on animal tissue-deep eutectic solvent-bamboo leaf powder in the present invention.

[0030] Figure 7 This is the release curve of the flavonoid component in the composite nursing dressing based on animal tissue-deep eutectic solvent-bamboo leaf powder in the present invention. DETAILED DESCRIPTION

[0031] The following is a detailed description of the present invention. Although specific embodiments of the present invention are shown, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments set forth herein. Rather, these embodiments are provided to enable a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0032] This specification and claims do not distinguish components based on differences in terminology, but rather on differences in their functions. The terms "including" and "comprising" used in this application are open-ended and should be interpreted as meaning "including but not limited to." The examples described later in this specification are preferred embodiments of the present invention and are intended to serve as general guidelines for the purposes of this specification and are not intended to limit the scope of the invention.

[0033] Example 1

[0034] During the preparation of animal and plant raw materials, the pig large intestine, pig stomach and pig aortic arch are cleaned, the grease is washed off and the pieces are cut into small pieces. The pieces are then crushed in a 200W tissue homogenizer at a mass ratio of animal tissue to normal saline of 1:1 for 1 hour. The tissue homogenate is then placed in a centrifuge at a speed of 5000 r / min and centrifuged for 10 minutes. The supernatant is discarded to obtain pig large intestine homogenate, pig stomach homogenate and pig aortic arch homogenate respectively. At the same time, the dried bamboo leaves are ultrafinely ground to obtain a powder with a particle size range of 80 to 200 mesh. The two types of raw materials are sealed and stored at 5°C for later use.

[0035] Example 2

[0036] (1) Weigh 0.75 g of a low eutectic solvent (choline chloride / acrylic acid, molar ratio 1:2) and mix with 0.15 g of porcine large intestine homogenate, stir evenly at room temperature, then add 0.0375 g of guar gum and mix thoroughly, then add 0.015 g of 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone and mix evenly; the mass ratio of the porcine large intestine homogenate to the low eutectic solvent is 1:5, the mass ratio of the guar gum to the low eutectic solvent is 1:20, and the mass ratio of the photoinitiator to the low eutectic solvent is 1:50;

[0037] (2) Pour the prepared membrane slurry into a smooth and clean mold and spread it evenly, controlling the thickness to 1.5 mm;

[0038] (3) Irradiate the slurry in the mold under a 6W ultraviolet lamp for 12 hours to completely solidify the slurry; after drying under reduced pressure at 50°C in a drying oven for 3 hours, a dressing base material of pig large intestine homogenate without bamboo leaf powder and low eutectic solvent can be obtained, which is then punched, sterilized, packaged, and sealed for storage.

[0039] Example 3

[0040] (1) Weigh 0.75 g of a low eutectic solvent (choline chloride / acrylamide, molar ratio 1:2) and mix with 0.15 g of porcine large intestine homogenate, stir evenly at room temperature, then add 0.0375 g of guar gum and mix thoroughly, then add 0.015 g of 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone and mix evenly; the mass ratio of the porcine large intestine homogenate to the low eutectic solvent is 1:5, the mass ratio of the guar gum to the low eutectic solvent is 1:20, and the mass ratio of the photoinitiator to the low eutectic solvent is 1:50;

[0041] (2) Pour the prepared membrane slurry into a smooth and clean mold and spread it evenly, controlling the thickness to 1.5 mm;

[0042] (3) Irradiate the slurry in the mold under a 6W ultraviolet lamp for 12 hours to completely solidify the slurry; after drying under reduced pressure at 50°C in a drying oven for 3 hours, a dressing base material of pig large intestine homogenate without bamboo leaf powder and low eutectic solvent can be obtained, which is then punched, sterilized, packaged, and sealed for storage.

[0043] Example 4

[0044] (1) Weigh 0.75 g of a low eutectic solvent (choline chloride / acrylic acid, molar ratio 1:2) and mix it with 0.15 g of porcine large intestine homogenate, stir it evenly at room temperature, then add 0.0375 g of guar gum and mix it thoroughly, and then add 0.015 g of 2-benzyl-2-dimethylamino-1-(4-morpholinophenyl)butanone and mix it evenly; the mass ratio of the porcine large intestine homogenate to the low eutectic solvent is 1:5, the mass ratio of the guar gum to the low eutectic solvent is 1:20, and the mass ratio of the photoinitiator to the low eutectic solvent is 1:50;

[0045] (2) Pour the prepared membrane slurry into a smooth and clean mold and spread it evenly, controlling the thickness to 1.5 mm;

[0046] (3) Irradiate the slurry in the mold under a 6W ultraviolet lamp for 12 hours to completely solidify the slurry; after drying under reduced pressure at 50°C in a drying oven for 3 hours, a dressing base material of pig large intestine homogenate without bamboo leaf powder and low eutectic solvent can be obtained, which is then punched, sterilized, packaged, and sealed for storage.

[0047] Example 5

[0048] (1) Weigh 0.75 g of a low eutectic solvent (choline chloride / acrylic acid, molar ratio 1:2) and mix with 0.3 g of porcine large intestine homogenate, stir evenly at room temperature, then add 0.0375 g of guar gum and mix thoroughly, then add 0.015 g of 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone and mix evenly; the mass ratio of the porcine large intestine homogenate to the low eutectic solvent is 2:5, the mass ratio of the guar gum to the low eutectic solvent is 1:20, and the mass ratio of the photoinitiator to the low eutectic solvent is 1:50;

[0049] (2) Pour the prepared membrane slurry into a smooth and clean mold and spread it evenly, controlling the thickness to 1.5 mm;

[0050] (3) Irradiate the slurry in the mold under a 6W ultraviolet lamp for 12 hours to completely solidify the slurry; after drying under reduced pressure at 50°C in a drying oven for 3 hours, a dressing base material of pig large intestine homogenate without bamboo leaf powder and low eutectic solvent can be obtained, which is then punched, sterilized, packaged, and sealed for storage.

[0051] Example 6

[0052] (1) Weigh 0.75 g of a low eutectic solvent (choline chloride / acrylic acid, molar ratio 1:2) and mix with 0.15 g of porcine large intestine homogenate, stir evenly at room temperature, then add 0.075 g of guar gum and mix thoroughly, then add 0.015 g of 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone and mix evenly; the mass ratio of the porcine large intestine homogenate to the low eutectic solvent is 1:5, the mass ratio of the guar gum to the low eutectic solvent is 1:10, and the mass ratio of the photoinitiator to the low eutectic solvent is 1:50;

[0053] (2) Pour the prepared membrane slurry into a smooth and clean mold and spread it evenly, controlling the thickness to 1.5 mm;

[0054] (3) Irradiate the slurry in the mold under a 6W ultraviolet lamp for 12 hours to completely solidify the slurry; after drying under reduced pressure at 50°C in a drying oven for 3 hours, a dressing base material of pig large intestine homogenate without bamboo leaf powder and low eutectic solvent can be obtained, which is then punched, sterilized, packaged, and sealed for storage.

[0055] Example 7

[0056] (1) Weigh 0.75 g of a low eutectic solvent (choline chloride / acrylic acid, molar ratio 1:2) and mix with 0.15 g of pig stomach homogenate, stir evenly at room temperature, then add 0.0375 g of guar gum and mix thoroughly, then add 0.015 g of 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone and mix evenly; the mass ratio of the pig stomach homogenate to the low eutectic solvent is 1:5, the mass ratio of the guar gum to the low eutectic solvent is 1:20, and the mass ratio of the photoinitiator to the low eutectic solvent is 1:50;

[0057] (2) Pour the prepared membrane slurry into a smooth and clean mold and spread it evenly, controlling the thickness to 1.5 mm;

[0058] (3) Irradiate the slurry in the mold under a 6W ultraviolet lamp for 12 hours to completely solidify the slurry; after drying under reduced pressure at 50°C in a drying oven for 3 hours, a dressing base material of pig stomach homogenate without bamboo leaf powder and low eutectic solvent can be obtained, which is then punched, sterilized, packaged, and sealed for storage.

[0059] Example 8

[0060] (1) Weigh 0.75 g of a low eutectic solvent (choline chloride / acrylic acid, molar ratio 1:2) and mix with 0.15 g of porcine aortic arch homogenate, stir evenly at room temperature, then add 0.0375 g of guar gum and mix thoroughly, then add 0.015 g of 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone and mix evenly; the mass ratio of the porcine aortic arch homogenate to the low eutectic solvent is 1:5, the mass ratio of the guar gum to the low eutectic solvent is 1:20, and the mass ratio of the photoinitiator to the low eutectic solvent is 1:50;

[0061] (2) Pour the prepared membrane slurry into a smooth and clean mold and spread it evenly, controlling the thickness to 1.5 mm;

[0062] (3) Irradiate the slurry in the mold under a 6W ultraviolet lamp for 12 hours to completely solidify the slurry; after drying under reduced pressure at 50°C in a drying oven for 3 hours, a dressing base material of pig aortic arch homogenate without bamboo leaf powder and low eutectic solvent can be obtained, which is then punched, sterilized, packaged, and sealed for storage.

[0063] Example 9

[0064] (1) Weigh 0.75 g of a low eutectic solvent (choline chloride / acrylic acid, molar ratio 1:2) and mix with 0.3 g of pig stomach homogenate, stir evenly at room temperature, then add 0.0375 g of guar gum and mix thoroughly, then add 0.015 g of 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone and mix evenly; the mass ratio of the pig stomach homogenate to the low eutectic solvent is 1:5, the mass ratio of the guar gum to the low eutectic solvent is 1:20, and the mass ratio of the photoinitiator to the low eutectic solvent is 1:50;

[0065] (2) Pour the prepared membrane slurry into a smooth and clean mold and spread it evenly, controlling the thickness to 1.5 mm;

[0066] (3) Irradiate the slurry in the mold under a 6W ultraviolet lamp for 12 hours to completely solidify the slurry; after drying under reduced pressure at 50°C in a drying oven for 3 hours, a dressing base material of pig stomach homogenate without bamboo leaf powder and low eutectic solvent can be obtained, which is then punched, sterilized, packaged, and sealed for storage.

[0067] Example 10

[0068] (1) Weigh 0.75 g of a low eutectic solvent (choline chloride / acrylic acid, molar ratio 1:2) and mix with 0.225 g of porcine aortic arch homogenate, stir evenly at room temperature, then add 0.0375 g of guar gum and mix thoroughly, then add 0.015 g of 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone and mix evenly; the mass ratio of the porcine aortic arch homogenate to the low eutectic solvent is 1:5, the mass ratio of the guar gum to the low eutectic solvent is 1:20, and the mass ratio of the photoinitiator to the low eutectic solvent is 1:50;

[0069] (2) Pour the prepared membrane slurry into a smooth and clean mold and spread it evenly, controlling the thickness to 1.5 mm;

[0070] (3) Irradiate the slurry in the mold under a 6W ultraviolet lamp for 12 hours to completely solidify the slurry; after drying under reduced pressure at 50°C in a drying oven for 3 hours, a dressing base material of pig aortic arch homogenate without bamboo leaf powder and low eutectic solvent can be obtained, which is then punched, sterilized, packaged, and sealed for storage.

[0071] Example 11

[0072] (1) Weigh 0.75 g of a low eutectic solvent (choline chloride / acrylic acid, molar ratio 1:2) and mix it with 0.3 g of pig large intestine homogenate, stir it evenly at room temperature, then add 0.0375 g of guar gum and mix it thoroughly, then add 0.015 g of 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone and mix it evenly, and finally add 0.0375 g of bamboo leaf powder and mix it thoroughly; the mass ratio of the pig large intestine homogenate to the low eutectic solvent is 2:5, the mass ratio of the guar gum to the low eutectic solvent is 1:20, the mass ratio of the photoinitiator to the low eutectic solvent is 1:50, and the mass ratio of the bamboo leaf powder to the low eutectic solvent is 1:20;

[0073] (2) Pour the prepared membrane slurry into a smooth and clean mold and spread it evenly, controlling the thickness to 1.5 mm;

[0074] (3) Irradiate the slurry in the mold under a 6W ultraviolet lamp for 12 hours to completely solidify the slurry; after drying under reduced pressure at 50°C in a drying oven for 3 hours, a composite nursing dressing of pig large intestine homogenate-low eutectic solvent-bamboo leaf powder can be obtained, which is then punched, sterilized, packaged, and sealed for storage.

[0075] Example 12

[0076] (1) Weigh 0.75 g of a low eutectic solvent (choline chloride / acrylic acid, molar ratio 1:2) and mix with 0.3 g of pig stomach homogenate, stir evenly at room temperature, then add 0.0375 g of guar gum and mix thoroughly, then add 0.015 g of 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone and mix thoroughly, and finally add 0.0375 g of bamboo leaf powder and mix thoroughly; the mass ratio of the pig stomach homogenate to the low eutectic solvent is 1:5, the mass ratio of the guar gum to the low eutectic solvent is 1:20, the mass ratio of the photoinitiator to the low eutectic solvent is 1:50, and the mass ratio of the bamboo leaf powder to the low eutectic solvent is 1:20;

[0077] (2) Pour the prepared membrane slurry into a smooth and clean mold and spread it evenly, controlling the thickness to 1.5 mm;

[0078] (3) Irradiate the slurry in the mold under a 6W ultraviolet lamp for 12 hours to completely solidify the slurry; after drying under reduced pressure at 50°C in a drying oven for 3 hours, a composite nursing dressing of pig stomach homogenate-low eutectic solvent-bamboo leaf powder can be obtained, which is then punched, sterilized, packaged, and sealed for storage.

[0079] Example 13

[0080] (1) Weigh 0.75 g of a low eutectic solvent (choline chloride / acrylic acid, molar ratio 1:2) and mix it with 0.225 g of porcine aortic arch homogenate, stir it evenly at room temperature, then add 0.0375 g of guar gum and mix it thoroughly, then add 0.015 g of 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone and mix it evenly, and finally add 0.0375 g of bamboo leaf powder and mix it thoroughly; the mass ratio of the porcine aortic arch homogenate to the low eutectic solvent is 1:5, the mass ratio of the guar gum to the low eutectic solvent is 1:20, the mass ratio of the photoinitiator to the low eutectic solvent is 1:50, and the mass ratio of the bamboo leaf powder to the low eutectic solvent is 1:20;

[0081] (2) Pour the prepared membrane slurry into a smooth and clean mold and spread it evenly, controlling the thickness to 1.5 mm;

[0082] (3) Irradiate the slurry in the mold under a 6W ultraviolet lamp for 12 hours to completely solidify the slurry; after drying under reduced pressure at 50°C in a drying oven for 3 hours, a composite nursing dressing of porcine aortic arch homogenate-low eutectic solvent-bamboo leaf powder can be obtained, which is then punched, sterilized, packaged, and sealed for storage.

[0083] The moldability of the substrates prepared in Examples 2 to 4 obtained by polymerization of animal tissue-deep eutectic solvent without adding functional bamboo leaf powder was compared. The results are shown in Table 1. In addition, the appearance comparison is shown in Table 2. Figure 1 .

[0084] Table 1. Comparison of overall properties of the dressing substrates of the animal tissue homogenate-deep eutectic solvent obtained in Examples 2 to 4.

[0085] Serial number Dressing properties 2 The color is light yellow, with good transparency, good film forming properties and good uniformity. 3 Yellowish color, poor transparency, poor film forming properties, and poor uniformity 4 The color is yellowish brown, the transparency is poor, the film forming property is good, and the uniformity is poor

[0086] Porosity test experiments were conducted on the substrates obtained by polymerization of animal tissue-deep eutectic solvent without adding the functional substance bamboo leaf powder prepared in Examples 2 and Examples 5 to 10. The results are shown in Table 2. The specific operation of the porosity test experiment is as follows:

[0087] Porosity experiment: The ethanol displacement method commonly used in this field was used, that is, the substrate was placed in a drying oven and dried until the weight no longer changed. The substrate was then immersed in a measuring cylinder filled with ethanol. The sample was taken out after 1 hour. The porosity calculation formula is P (%) = (W2-W1) / (d×V), where W2 is the mass of the sample after ethanol immersion, W1 is the mass of the dry sample, d is the density of ethanol, and V is the sample volume.

[0088] The air permeability test was conducted on the substrates obtained by polymerization of animal tissue and deep eutectic solvent without adding the functional ingredient bamboo leaf powder prepared in Examples 2 and Examples 5 to 10. Specifically, the air permeability of the composite dressing film was measured based on the measurement of water vapor transmission rate (WVTR). The results are shown in Table 2. The specific experimental procedures are as follows:

[0089] Air permeability test: use an area of ​​1cm 2 The cuvette was filled with 3 mL of deionized water, and the composite nursing dressing was cut into a suitable size and covered on the surface of the cuvette to completely seal it. The cuvette was then placed in a dry incubator at a temperature of 37°C and a relative humidity of 20%. The weight of the cuvette was accurately measured every hour, and a graph of the relationship between water evaporation and time was drawn. The slope of the curve can be known from the curve. The WVTR value is calculated as WVTR = S × 24 / A, where S is the slope of the water evaporation mass and time, and A is the area of ​​the cuvette (cm 2 ).

[0090] Table 2. Comparison of porosity and water vapor permeability of the animal tissue-deep eutectic solvent dressing substrates obtained in Example 2 and Examples 5 to 10.

[0091] Example Porosity <![CDATA[Water vapor transmission rate / g / (m 2 ·day)]]> 2 19.46％ 2207.01 5 28.85％ 2546.55 6 16.42％ 1973.58 7 16.82％ 2461.67 8 14.92％ 2185.79 9 26.64％ 2461.67 10 20.32％ 2440.45

[0092] The tensile test was conducted on the substrate obtained by polymerizing the animal tissue-deep eutectic solvent without adding the functional substance bamboo leaf powder prepared in Example 2. Figure 2 , it can be seen that it is not easy to break and has strong elasticity.

[0093] The bonding experiment was conducted on the substrate obtained by polymerizing the animal tissue-deep eutectic solvent without adding the functional substance bamboo leaf powder prepared in Example 2. The results are shown in the attached Figure 3It can be seen that it has obvious adhesion and fitting properties on different surfaces.

[0094] The morphology of the composite nursing dressing of animal tissue-deep eutectic solvent-bamboo leaf powder prepared in Example 11 was characterized by scanning electron microscopy. The results are shown in the attached Figure 4 It can be seen that the color of the dressing with bamboo leaf powder added is significantly darker, but its surface uniformity has not changed significantly.

[0095] The composite nursing dressing of animal tissue-deep eutectic solvent-bamboo leaf powder prepared in Example 11 was tested by infrared spectroscopy. Figure 5 , it can be seen that the characteristic signals of the main components are all included.

[0096] The stress-tensile curve of the composite nursing dressing of animal tissue-deep eutectic solvent-bamboo leaf powder prepared in Example 11 was tested using a universal tensile testing machine. The results are shown in the attached Figure 6 , it can be seen that its mechanical properties are good.

[0097] The composite nursing dressings of animal tissue, deep eutectic solvent and bamboo leaf powder prepared in Examples 11 to 13 were subjected to moisture absorption and moisturizing experiments. The results are shown in Table 3. The specific operation is as follows:

[0098] Moisture absorption test: The composite nursing dressing was placed in an oven and dried at 105°C for 3 hours, then placed in a silica gel desiccator and cooled to room temperature; the dried dressing was then placed in an incubator at 25°C and 85% relative humidity, and the weight of the dressing was measured after 24 hours; the moisture absorption rate was calculated as R a =100%×(W1-W0) / W0, where W1 is the mass of the composite dressing after 24 hours of placement, and W0 is the mass of the composite dressing before 24 hours of placement.

[0099] Moisturizing experiment: The composite nursing dressing was dried at 105°C for 3 hours, then placed in a silica gel desiccator and cooled to room temperature. 10% by mass of water was added to the sample after drying to constant weight. The dressing was then placed in an incubator at 25°C and 15% relative humidity, and the weight of the dressing was measured after 48 hours. The formula for calculating the moisturizing rate is R h =100%×H1 / H0, where H1 is the mass of the composite dressing after 48 hours of placement, and H0 is the mass of the composite dressing before 24 hours of placement.

[0100] Table 3, Comparison of moisture absorption and moisturizing data of the composite nursing dressings of animal tissue-deep eutectic solvent-bamboo leaf powder prepared in Examples 11 to 13.

[0101] Serial number Moisture absorption rate Moisturizing rate 11 40.35％ 46.43％ 12 38.42％ 47.83％ 13 44.37％ 52.38％

[0102] The composite nursing dressing of animal tissue-deep eutectic solvent-bamboo leaf powder loaded with bamboo chlorophyll prepared in Example 11 was measured based on the recognized Franz diffusion cell method for the cumulative permeation of the active ingredient of bamboo leaf flavonoids; the permeation membrane was selected as a medical PDMS membrane with a thickness of 0.5 mm; it was soaked in 95% ethanol for 30 min before use and then naturally evaporated for use; the flavonoid content was determined by the method in the "Chinese Pharmacopoeia" by gradually adding 5% sodium nitrite, 10% aluminum nitrate, and 1 mol / L sodium hydroxide to the sample and then using an ultraviolet spectrophotometer at 510 nm. A working curve was established using rutin as the standard; the trend of the cumulative permeation of bamboo leaf flavonoids over time is shown in the attached figure. Figure 7 , it can be seen that the overall release time is more than 48h, that is, the composite dressing shows the expected sustained-release effect.

Claims

1. A composite nursing dressing based on animal tissue-deep eutectic solvent-bamboo leaf powder, characterized in that: The main components are animal tissue, low eutectic solvent and bamboo leaf powder, followed by guar gum and photoinitiator.

2. The composite nursing dressing based on animal tissue-deep eutectic solvent-bamboo leaf powder according to claim 1, characterized in that: The dressing matrix used is animal tissue homogenate rich in elastic fibers, including large intestine homogenate, stomach homogenate or aortic arch homogenate.

3. The composite nursing dressing based on animal tissue-deep eutectic solvent-bamboo leaf powder according to claim 1, characterized in that: The deep eutectic solvent used includes choline chloride-acrylic acid (molar ratio of 1:2) or choline chloride-acrylamide (molar ratio of 1:2), which serves as a polymerization monomer, a functional solvent, and a penetration enhancer.

4. The composite nursing dressing based on animal tissue-deep eutectic solvent-bamboo leaf powder according to claim 1, characterized in that: The bamboo leaf powder used is a functional substance that can be added. It is obtained by ultrafine grinding of dried bamboo leaves and has a particle size range of 80 to 200 meshes.

5. The composite nursing dressing based on animal tissue-deep eutectic solvent-bamboo leaf powder according to claim 1, characterized in that: The dressing forming agent used is guar gum.

6. The composite nursing dressing based on animal tissue-deep eutectic solvent-bamboo leaf powder according to claim 1, characterized in that: The photoinitiator used is 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone or 2-benzyl-2-dimethylamino-1-(4-morpholinophenyl)butanone.

7. The composite nursing dressing based on animal tissue-deep eutectic solvent-bamboo leaf powder according to claim 1, characterized in that: The animal tissue is crushed and homogenized, and the obtained animal tissue homogenate is first mixed with a deep eutectic solvent, and then guar gum, a photoinitiator, and bamboo leaf powder are added in sequence, stirred thoroughly, and polymerized under ultraviolet light. Then, a finished composite nursing dressing is prepared through molding, drying, sterilization, and packaging. The specific steps are as follows: (1) Clean the large intestine, stomach, or aortic arch, wash off the fat, cut into small pieces, and crush in a 200W tissue homogenizer at a mass ratio of animal tissue to physiological saline of 1:1 for 1 hour; then centrifuge the tissue homogenate at a speed of 5000 r / min for 10 minutes, discard the supernatant, and obtain the large intestine homogenate, stomach homogenate, or aortic arch homogenate respectively; (2) animal tissue homogenate: deep eutectic solvent = 1:5 to 2:5 mass ratio is mixed uniformly, then guar gum is added according to the mass ratio of guar gum: deep eutectic solvent = 1:20 to 1:10 mass ratio and mixed uniformly, then photoinitiator is added according to the mass ratio of photoinitiator: deep eutectic solvent = 1:50 mass ratio and mixed uniformly to form a film slurry, finally bamboo leaf powder is added to the film slurry according to the mass ratio of bamboo leaf powder: deep eutectic solvent = 1:20 mass ratio and mixed uniformly; (3) Pour the prepared membrane slurry into a smooth and clean mold, spread it evenly, and control the thickness to be 1-2 mm; (4) Irradiate the slurry in the mold under a 6W ultraviolet lamp for 12 to 24 hours. Stop irradiation after the slurry is completely solidified. (5) Place the prepared dressing in a drying oven and dry it under reduced pressure at 50°C for 3 hours before taking it out; (6) Sterilize the dried dressing by irradiating it with ultraviolet light for 60 minutes; (7) Cut the prepared composite nursing dressing into regular shapes, package them, and seal them for storage.

Images