Nucleic acid detection equipment

Abstract

The invention discloses nucleic acid detection equipment which comprises an xy platform module, a rack module and an operation platform module, the xy platform module is provided with a bottom plate, a first guide rail in the x direction and a second guide rail in the y direction, and the first guide rail and the second guide rail are located above the bottom plate; the rack module comprises a base, a side plate, a power supply and a main control board, the side plate is detachably connected to the bottom plate, and an optical module, a gland module, an extraction module and a pipetting module are detachably connected to the base; the operation platform module is located above the xy platform module, the operation platform module is provided with a kit used for molecular extraction, a micro-fluidic chip used for gene amplification, a temperature control module used for PCR reaction and a driving control board capable of controlling the temperature of the temperature control module, and the main control board controls the operation platform module to move. Driving of other consumable transferring mechanisms is not needed, the situation that the internal structure of the PCR all-in-one machine is too complex, and consequently the size is large is avoided, and the PCR all-in-one machine can be used in places with harsh requirements for the size and space.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection, and in particular to a nucleic acid detection device. Background Art

[0002] The PCR all-in-one machine is a fully automatic nucleic acid detection device that integrates the entire process of nucleic acid extraction, reaction system preparation, PCR amplification detection, and reporting results, and can complete the detection without human intervention.

[0003] However, most existing all-in-one PCR machines use the reciprocating movement of a robot to transfer samples between different modules for subsequent gene amplification and testing. The robot and the mobile structure for the reciprocating movement of the robot require a large amount of internal space, which makes the all-in-one PCR machine larger and unsuitable for laboratories, bedside areas, outpatient clinics and other places with strict requirements on volume and space.

[0004] Therefore, how to avoid the large size of the PCR all-in-one machine due to its overly complex internal structure so as to meet the use in places with more stringent requirements on volume and space is a technical problem that technical personnel in this field currently need to solve. Summary of the Invention

[0005] The purpose of the present invention is to provide a nucleic acid detection device that avoids the large size caused by the overly complex internal structure of the PCR all-in-one machine, so as to meet the use in places with more stringent requirements on volume space.

[0006] To achieve the above objectives, the present invention provides a nucleic acid detection device, comprising:

[0007] An xy platform module is provided with a base plate and a first guide rail in the x direction and a second guide rail in the y direction located above the base plate;

[0008] The rack module includes a base, side panels, a power supply and a main control panel. The side panels are detachably connected to the bottom panel. The optical module, the capping module, the extraction module and the pipetting module are detachably connected to the base.

[0009] The operating platform module is located above the xy platform module and is movably connected to the first guide rail and the second guide rail. The operating platform module is provided with a reagent kit for molecular extraction, a microfluidic chip for gene amplification, a temperature control module for PCR reaction and a drive control board. The drive control board can control the temperature of the temperature control module. The main control board is used to control the operation platform module to move to the bottom of the optical module, the capping module, the extraction module and the pipetting module.

[0010] Preferably, the operating platform module further includes:

[0011] The gun tip seat is arranged between the reagent box and the chip. The pipetting module transfers the sample liquid on the reagent box to the chip through the gun tip in the gun tip seat.

[0012] Preferably, the temperature control module includes a cooling plate, which can exchange heat with the chip.

[0013] Preferably, a cooling fan is provided at the bottom of the cooling fin, which is used to circulate air to the cooling fin to cool the cooling fin. An exhaust channel for air discharge is provided on one side of the cooling fin. The exhaust channel includes a first section extending to the xy platform module and a second section arranged at an angle to the first section to allow air to circulate to the outside of the xy platform module.

[0014] Preferably, a first opening is provided on a side of the refrigeration fin facing away from the exhaust passage, and a second opening corresponding to the first opening is provided on the side plate.

[0015] Preferably, a purge module is further included, and the purge module includes:

[0016] A drive assembly, comprising an air inlet fan and an air outlet fan connected to and located above the base;

[0017] A laminar flow hood includes a first air duct having a first air cavity and a second air duct having a second air cavity, wherein one end of each of the first air duct and the second air duct is connected to the bottom of the base, and an air inlet of the first air cavity corresponds to an air inlet fan, and an air outlet of the second air cavity corresponds to an air outlet fan, the air outlet of the first air cavity is located at the other end of the first air duct, and the air inlet of the second air cavity is arranged opposite to the air outlet of the first air cavity;

[0018] The filter assembly includes a first filter and a second filter. The first filter is arranged at the air outlet of the first air cavity, and the second filter is arranged at the air inlet of the second air cavity, so that a laminar wind blowing layer is formed between the air outlet of the first air cavity and the air inlet of the second air cavity, and the laminar wind blowing layer is located below the extraction module and the pipetting module.

[0019] Preferably, it further comprises a shell arranged outside the rack module, the shell is provided with a door for the operating platform module to enter and exit the interior of the rack module, and the door is rotatably connected to the shell through an opening and closing mechanism.

[0020] Preferably, the opening and closing mechanism includes:

[0021] A fixing part connected to the warehouse door;

[0022] A hinge plate is provided on the housing and is located below the door;

[0023] An elastic member has one end connected to the fixing member and the other end connected to the hinge plate. The elastic member is used for automatically closing the door.

[0024] Preferably, the operating platform module is provided with a first roller and a second roller at different heights, and the first roller and the second roller can successively abut against the warehouse door to drive the warehouse door to rotate downward.

[0025] Preferably, the operating platform module is provided with a first fixed plate, and the rack module is provided with a second fixed plate at the same height as the first fixed plate, and the two ends of the drag chain are respectively fixed to the first fixed plate and the second fixed plate to enable the wires of the operating platform module to be transferred to the power supply and main control board of the rack module.

[0026] Preferably, a first threaded hole is provided on the side panel, a second threaded hole corresponding to the first threaded hole is provided on the support seat of the operating platform module, the connecting piece is movably passed through the first threaded hole, and the connecting piece is provided with a spring abutting against the side panel, the connecting piece is provided with a threaded section, and the threaded section can be screwed into the second threaded hole to put the spring in a compressed state.

[0027] Relative to the above background technology, the nucleic acid detection equipment provided by the present invention includes an xy platform module, a rack module and an operating platform module, the xy platform module is provided with a bottom plate and a first guide rail in the x direction and a second guide rail in the y direction located above the bottom plate; the rack module includes a base, a side panel, a power supply and a main control panel, the side panel is detachably connected to the bottom plate, and the base is detachably connected to an optical module, a capping module, an extraction module and a pipetting module; the operating platform module is located above the xy platform module and is movably connected to the first guide rail and the second guide rail, the operating platform module is provided with a reagent kit for molecular extraction, a microfluidic chip for gene amplification, a temperature control module for PCR reaction and a drive control panel, the drive control panel can control the temperature of the temperature control module, and the main control panel is used to control the operating platform module to move below the optical module, the capping module, the extraction module and the pipetting module.

[0028] Specifically, the reagent kit for molecular extraction, the microfluidic chip for gene amplification, the temperature control module for PCR reaction and the drive control board are integrated into the operating platform module, and the optical module, the capping module, the extraction module and the pipetting module are integrated into the base of the rack module. Under the control of the main control board, the operating platform module can move the reagent kit to the bottom of the extraction module for nucleic acid extraction, move it to the bottom of the pipetting module for sample liquid transfer, move the microfluidic chip to the bottom of the capping module for gene amplification, and move the amplified microfluidic chip to the optical module for nucleic acid detection, thereby realizing the coordinate movement on the plane of the processed object. In conjunction with the capping module, pipetting module, extraction module and optical module with fixed plane positions, there is no need to add the drive of other transfer consumables mechanisms, thereby realizing the modular combination design of the entire equipment, avoiding the large size caused by the overly complex internal structure of the PCR all-in-one machine, so as to meet the use in places with more stringent volume space requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are merely embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying any creative work.

[0030] Figure 1 A schematic diagram of the structure of a nucleic acid detection device provided in an embodiment of the present invention;

[0031] Figure 2 for Figure 1 The main view;

[0032] Figure 3 for Figure 1 Side view of

[0033] Figure 4 for Figure 1 A top view of

[0034] Figure 5 A schematic diagram of the structure of the operating platform module provided in an embodiment of the present invention;

[0035] Figure 6 A cross-sectional view of the structure of a refrigeration fin provided by an embodiment of the present invention;

[0036] Figure 7 A schematic structural diagram of a warehouse door provided in an embodiment of the present invention;

[0037] Figure 8 A schematic structural diagram of a warehouse door in another state provided by an embodiment of the present invention;

[0038] Figure 9 A schematic diagram of the structure of a drag chain provided by an embodiment of the present invention;

[0039] Figure 10 This is a schematic diagram of the structure of the operating platform module and the drag chain provided by the embodiment of the present invention in different positions;

[0040] Figure 11 A schematic structural diagram of a connector provided in an embodiment of the present invention;

[0041] Figure 12 A schematic diagram of the structure of the purge module provided in an embodiment of the present invention being installed on a base;

[0042] Figure 13 A schematic structural diagram of a purge module provided in an embodiment of the present invention;

[0043] Figure 14A schematic diagram of the structure of the operating platform module provided in an embodiment of the present invention when it is moved below the purge module;

[0044] Figure 15 A schematic diagram of the structure of the drive assembly provided by an embodiment of the present invention being installed on a base;

[0045] Figure 16 A cross-sectional view of the operating platform module provided in an embodiment of the present invention when it is moved below the purge module;

[0046] Figure 17 This is a structural diagram of the code scanner and button switch provided in an embodiment of the present invention.

[0047] in:

[0048] 100-xy platform module, 110-base plate;

[0049] 200 - rack module, 210 - base, 220 - side panel, 221 - second opening, 230 - second fixing plate;

[0050] 300-optical module;

[0051] 400-capping module;

[0052] 500- pipetting module, 510- pipette tip;

[0053] 600-operation platform module, 610-reagent kit, 611-extraction area, 612-freeze-drying area, 620-chip, 630-drive control board, 640-gun head seat, 651-refrigeration plate, 652-cooling fan, 653-exhaust channel, 660-first roller, 670-second roller, 680-first fixing plate, 690-support base;

[0054] 700-housing, 710-door, 720-fixing part, 730-hinge plate, 740-elastic part;

[0055] 800-drag chain;

[0056] 900-connector, 910-spring;

[0057] 1000-extraction module, 1010-extraction gun tip;

[0058] 1100-intake fan;

[0059] 1200-air outlet fan;

[0060] 1300 - laminar flow hood, 1310 - first air duct, 1311 - first air cavity, 1320 - second air duct, 1321 - second air cavity, 1330 - first filter, 1340 - second filter;

[0061] 1400-barcode scanner;

[0062] 1500-push button switch. DETAILED DESCRIPTION

[0063] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0064] In order to enable those skilled in the art to better understand the solutions of the present invention, the present invention is further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0065] In the description of the present invention, it should be understood that the terms "top", "bottom", "inside", "outside", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the positions or elements referred to must have a specific orientation, be constructed and operate in a specific orientation. Therefore, they should not be understood as limitations of the present invention.

[0066] The purpose of the present invention is to provide a nucleic acid detection device that avoids the large size caused by the overly complex internal structure of the PCR all-in-one machine, so as to meet the use in places with more stringent requirements on volume space.

[0067] See also Figures 1 to 4 To achieve the above objectives, the present invention provides a nucleic acid detection device, including an xy platform module 100, a rack module 200 and an operating platform module 600.

[0068] The xy platform module 100 includes a base plate 110 and a first guide rail in the x direction and a second guide rail in the y direction located above the base plate 110 , wherein the x direction and the y direction are arranged at an angle.

[0069] The rack module 200 includes a base 210 and side panels 220, a power supply, and a main control board. The side panels 220 are detachably connected to the bottom plate 110 so that the base 210 is spaced above the bottom plate 110. The optical module 300, the capping module 400, the extraction module 1000, and the pipetting module 500 are detachably connected to the base 210 so that the optical module 300, the capping module 400, the extraction module 1000, and the pipetting module 500 are separated from each other above and below the bottom plate 110.

[0070] See also Figure 5The operating platform module 600 is located above the xy platform module 100 and is movably connected to the first guide rail and the second guide rail to enable the operating platform module 600 to move to various preset positions on the base plate 110. The operating platform module 600 is provided with a reagent kit 610 for molecular extraction, a microfluidic chip 620 for gene amplification, a temperature control module for PCR reaction, and a drive control board 630, so that the processed objects are all on the same platform and do not need to be transferred, so as to avoid setting up unnecessary transfer mechanisms for the equipment and save the internal space of the equipment. The drive control board 630 can control the temperature of the temperature control module, and can realize heating, cooling and precise temperature control of the temperature control module. The main control board can control the operating platform module 600 to move to the bottom of the optical module 300, the capping module 400, the extraction module 1000 and the pipetting module 500 to perform the corresponding process.

[0071] The reagent kit 610 is composed of an extraction area 611 and a system freeze-drying area 612, and the reagent kit 610 adopts a closed anti-pollution design so that no zoning management is required within the device.

[0072] The reagent kit 610 for molecular extraction, the microfluidic chip 620 for gene amplification, the temperature control module for PCR reaction and the drive control board 630 are integrated into the operation platform module 600. At the same time, the optical module 300, the capping module 400, the extraction module 1000 and the pipetting module 500 are integrated into the base 210 of the rack module 200. Under the control of the main control board, the operation platform module 600 can move the reagent kit 610 to the bottom of the extraction module 1000 for nucleic acid extraction, move it to the bottom of the pipetting module 500 for sample liquid transfer, and move the microfluidic chip 620 to the bottom of the extraction module 1000 for nucleic acid extraction, and move the microfluidic chip 620 to the bottom of the pipetting module 500 for sample liquid transfer. 620 moves to the bottom of the capping module 400 for gene amplification, and the amplified microfluidic chip 620 can be moved to the optical module 300 for nucleic acid detection, realizing the coordinate movement on the plane of the processed object, and cooperating with the capping module 400, pipetting module 500, extraction module 1000 and optical module 300 whose plane positions are fixed, without the need to increase the drive of other transfer consumables mechanisms, realizing the modular combination design of the entire equipment, avoiding the large size caused by the overly complex internal structure of the PCR all-in-one machine, so as to meet the use of places with more stringent requirements on volume space.

[0073] In this embodiment, the operating platform module 600 also includes a gun tip seat 640, which is arranged between the test kit 610 and the chip 620. The pipetting module 500 transfers the sample liquid on the test kit 610 to the chip 620 through the gun tip on the gun tip seat 640. The gun tip seat 640 and the consumable gun tip are arranged adjacent to the test kit 610 and the chip 620. The movement of the operating platform module 600 can drive the gun tip to a preset position while saving internal space of the equipment.

[0074] The temperature control module can control the temperature of the chip 620 after being pressed and sealed by the cover module 400 to achieve gene amplification. The temperature control module includes a cooling plate 651 for heating the chip 620. The cooling plate 651 can exchange heat with the chip 620. When heating is required, the cooling plate 651 can heat the chip. When cooling is required, the heat of the chip 620 is absorbed by the cooling plate 651.

[0075] When the chip 620 performs a PCR reaction, the operating platform module 600 moves to the bottom of the capping module 400. The cap in the capping module 400 is pressed down to seal. After the pressure is replenished in the PCR reaction chamber, the temperature control module starts to heat and cool the chip 620.

[0076] See also Figure 6 A cooling fan 652 is provided at the bottom of the cooling fin 651. When the temperature of the cooling fin 651 rises, the cooling fan 652 starts to work and draws air from the bottom to circulate air to the cooling fin 651 to cool the cooling fin 651. An exhaust channel 653 for air exhaust is provided on one side of the cooling fin 651. The exhaust channel 653 includes a first section extending to the xy platform module 100 and a second section arranged at an angle to the first section to allow air to circulate to the outside of the xy platform module 100.

[0077] Specifically, the air from one side of the fin of the cooling plate 651 is first circulated along the first section to the xy platform module 100 through the exhaust channel 653 , and then circulated along the second section to the outside of the device.

[0078] In order to improve the heat dissipation effect of the cooling fin 651, a first opening is opened on the cooling fin 651 adjacent to the side panel 220, and the first opening is located on the side of the cooling fin 651 away from the exhaust channel 653, and a second opening 221 corresponding to the first opening is provided on the side panel 220, so that the air on the other side of the fin of the cooling fin 651 is directly discharged to the outside of the equipment along the first opening and the second opening 221, so as to improve the heat dissipation efficiency of the cooling fin 651.

[0079] See also Figure 12 、 Figure 13 、 Figure 14 、 Figure 15 and Figure 16The nucleic acid detection equipment also includes a purge module, which includes a drive assembly, a laminar flow hood 1300 and a filter assembly. The drive assembly includes an air inlet fan 1100 and an air outlet fan 1200 connected to the base 210 and located above the base 210; the laminar flow hood 1300 includes a first air duct 1310 provided with a first air cavity 1311 and a second air duct 1320 provided with a second air cavity 1321. One end of the first air duct 1310 and the second air duct 1320 are both connected to the bottom of the base 210, and the air inlet of the first air cavity 1311 corresponds to the air inlet fan 1100, and the air outlet of the second air cavity 1321 corresponds to the bottom of the base 210. In response to the air outlet fan 1200, the air outlet of the first air cavity 1311 is located at the other end of the first air duct 1310, and the air inlet of the second air cavity 1321 is arranged opposite to the air outlet of the first air cavity 1311; the filter assembly includes a first filter 1330 and a second filter 1340, the first filter 1330 is arranged at the air outlet of the first air cavity 1311, and the second filter 1340 is arranged at the air inlet of the second air cavity 1321, so that a laminar wind blowing layer is formed between the air outlet of the first air cavity 1311 and the air inlet of the second air cavity 1321, and the laminar wind blowing layer is located below the extraction module 1000 and the pipetting module 500.

[0080] The first filter 1330 and the second filter 1340 can be, but are not limited to, high-efficiency filters. A first filter cover for installing and removing the first filter 1330 is installed on the first air duct 1310, and a second filter cover for installing and removing the second filter 1340 is installed on the second air duct 1320. The air outlet of the first air cavity 1311 and the air inlet of the second air cavity 1321 are opened on the side of the first air duct 1310 and the second air duct 1320 facing each other, so that the air flow out of the air outlet of the first air cavity 1311 flows into the air inlet of the second air cavity 1321; the air inlet fan 1100 is used to allow air to enter the first air cavity 1311, and the air outlet fan 1200 is used to allow air to enter the second air cavity 1321. 1, thus forming an air circulation path. Driven by the air inlet fan 1100, the air flows along the first air cavity 1311 to the air inlet of the first air cavity 1311 and is discharged after passing through the first filter 1330. Thereafter, the air enters the air inlet of the second air cavity 1321 through the second filter 1340 and flows along the second air cavity 1321 before being discharged under the drive of the air outlet fan 1200. The laminar flow hood 1300 is U-shaped as a whole. The air inlet fan 1100 and the air outlet fan 1200 are arranged on the upper layer of the base 210. It can be set to self-circulate or be used for heat dissipation of the whole machine. The laminar flow hood 1300 covers the area where the extraction module 1000, the pipetting module 500 and the operation platform module 600 interact with each other.

[0081] During the process of nucleic acid extraction through the extraction tip 1010 of the extraction module 1000 and sample loading through the pipette tip 510 of the pipetting module 500, the operation platform module 600 carries the sample reagent box 610 to perform nucleic acid extraction, system construction and chip loading under the extraction module 1000 and the pipetting module 500. By adding a laminar flow hood 1300, a laminar wind sweep layer of 0.5-1mm / s is formed in the upper area of ​​the reagent box 610 to avoid the fine gas replacement inside and outside the closed reagent box 610 under the action of mixing and aspirating liquid by the extraction tip 1010, which leads to cross-contamination of sample aerosols, and the overflowed aerosol will be adsorbed by the second filter 1340 to avoid aerosol residual contamination and aerosol discharge.

[0082] See also Figure 7 、 Figure 8 and Figure 17 In this embodiment, the nucleic acid detection device also includes a shell 700 arranged on the outside of the rack module 200. The shell 700 is provided with a compartment door 710 for the operating platform module 600 to enter and exit the interior of the rack module 200. The compartment door 710 is rotatably connected to the shell 700 through an opening and closing mechanism. The shell 700 is also provided with a code scanner 1400 and a button switch 1500 on the same side as the compartment door 710. The code scanner 1400 can be used for scanning and identifying the test kit 610, and the button switch 1500 can be used to control whether the nucleic acid detection device is in operation or not.

[0083] The setting of the warehouse door 710 facilitates the operation platform module 600 to enter and exit the rack module 200, so as to be used for the placement of the nucleic acid detection kit 610 to be tested and the addition and replacement of consumables such as gun tips. Considering the convenience of moving the operation platform module 600 out of the warehouse door 710, a first roller 660 is provided on the side of the operation platform module 600 facing the warehouse door 710, and at the same time, a second roller 670 is provided on the operation platform module 600 at a different height from the above-mentioned first roller 660. When the operation platform module 600 contacts the warehouse door 710, the first roller 660 first abuts against the warehouse door 710 and drives the warehouse door 710 to rotate downward by a certain angle. Then, the second roller 670 abuts against the warehouse door 710 to realize the movement of the operation platform module 600 out of the rack module 200. Through the setting of the first roller 660 and the second roller 670, wear and / or collision caused by direct contact between other parts of the operation platform module 600 and the warehouse door 710 is avoided.

[0084] It should be noted that the opening and closing mechanism includes a fixing part 720 connected to the warehouse door 710, a hinge plate 730 arranged on the shell 700 and located below the warehouse door 710, and an elastic part 740 connected to the fixing part 720 at one end and to the hinge plate 730 at the other end. The elastic part 740 is used to automatically close the warehouse door 710.

[0085] The relative position of the connection end of the elastic member 740 and the fixing member 720 remains unchanged. When the warehouse door 710 rotates, the hinge plate 730 is driven to rotate, causing the other end of the elastic member 740 to swing, generating elastic force, and pushing the warehouse door 710 to flip open under the drive of the first roller 660 and the second roller 670; when the operating platform module 600 returns to the cabin, under the elastic force of the elastic member 740, the hinge plate 730 drives the warehouse door 710 to close.

[0086] The elastic member 740 may be a torsion spring, and the magnitude of the torsion force that the torsion spring can withstand may be set according to actual needs as long as it can achieve the above-mentioned purpose.

[0087] Considering that in the prior art, the setting of the drag chain 800 is usually to transfer one group of drag chains 800 to one group of baseboards, and then transfer them to another group of baseboards through another group of drag chains 800, so that the two groups of drag chains 800 can transfer the wires to the power supply and main control board on the rack module 200. The setting of the drag chain 800 is relatively complicated and increases the width of the equipment.

[0088] See also Figure 9 In this embodiment, a first fixing plate 680 is provided on the operating platform module 600, and a second fixing plate 230 at the same height as the first fixing plate 680 is provided on the rack module 200. Both ends of a set of drag chains 800 are fixed in parallel to the first fixing plate 680 and the second fixing plate 230, so that the drag chains 800 are always at the same height (moving in the same horizontal plane) to achieve the transfer of the wires of the operating platform module 600 to the power supply and main control board of the rack module 200. While reducing the number of drag chains 800, it can meet the demand for the transfer of the wires of the operating platform module 600 to the power supply and main control board of the rack module 200, so as to avoid the increase in the width of the equipment due to the excessive setting of drag chains 800.

[0089] See also Figure 10 , where B is the non-moving area of ​​the operating platform module 600 on the xy platform module 100, A1 and A2 are two states of the operating platform module 600 moving on the xy platform module 100, a1 is the position of the drag chain 800 when the operating platform module 600 moves to A1, a2 is the position of the drag chain 800 when the operating platform module 600 moves to A2, A3 is the state of the operating platform module 600 when it is out of the warehouse, a3 is the position of the drag chain 800 when the operating platform module 600 moves to A3, and a single drag chain 800 can meet the needs of connecting the wires of the operating platform module 600 to the power supply and main control board of the rack module 200 during the movement of each position.

[0090] See also Figure 11In this embodiment, a first threaded hole is provided on the side plate 220, and a second threaded hole corresponding to the first threaded hole is provided on the support seat 690 of the operating platform module 600. The connecting member 900 is movably inserted into the first threaded hole, and the connecting member 900 is sleeved with a spring 910 that abuts against the side plate 220. The connecting member 900 is provided with a threaded section, and the threaded section can be screwed into the second threaded hole to put the spring 910 in a compressed state.

[0091] Among them, the connecting piece 900 can be a captive screw. The specific process is that the captive screw passes through the spring 910 and is screwed through the first threaded hole on the side plate 220. Under the elastic force of the spring 910, it maintains a free state. A hexagonal wrench is inserted into the wrench hole on the head of the captive screw. Under the thrust of the wrench, the captive screw moves forward and contacts the second threaded hole provided on the support seat 690 of the operating platform module 600. The wrench is tightened to achieve the fixation of the side plate 220 and the support seat 690 of the operating platform module 600. During transportation, the pre-tightening force of the thread pair can prevent damage to parts caused by transportation vibration. When it is necessary to release the "locked" state for transportation, just insert the wrench into the head of the captive screw and turn it counterclockwise to loosen the thread. This solution has a secure fixation, takes up little space, and is easy to operate. In the "unlocked" state, the captive screw does not shake even when the spring is pre-compressed, and will not cause occasional vibration and abnormal noise during equipment operation.

[0092] In summary, the reagent box 610, the gun head holder 640 and the microfluidic chip 620 are arranged on the same operating platform module 600. The platform integrates a magnetic steel moving module (to drive the movement of the magnetic beads in the reagent box 610) and a temperature control module. The operating platform module 600 is placed on the bottom plate 110, which realizes the coordinate movement on the plane of the processing object. In conjunction with the capping module 400, the pipetting module 500, the extraction module 1000 and the optical module 300 whose plane positions are fixed, an optimal driving solution is achieved without adding other transfer consumables mechanisms; through the operating platform module 60 0 in and out of the warehouse as a drive, pushing the warehouse door 710 to open. When the operating platform module 600 enters the warehouse, the warehouse door 710 is automatically closed, reducing the additional driving structure for opening and closing the warehouse door 710; the operating platform module 600 has a built-in temperature control module, which adopts the method of bottom air intake and hot air exhaust on both sides. When the chip 620 performs PCR reaction, the operating platform module 600 is located below the capping module 400. The position of the operating platform module 600 is relatively fixed and close to the edge of the device, so that when the temperature control module is working, one side of the air is discharged to the outside of the device, and the other side is exhausted through the exhaust channel 653 is guided to be discharged from the bottom of the equipment to avoid hot air being directly discharged into the interior of the equipment, which causes the internal temperature to rise; there are a large number of wires on the operating platform module 600, which need to be connected to the power supply and main control board on the rack module 200 through the drag chain 800. The drag chain 800 is arranged in a direction parallel to the horizontal plane, so that the extension space of the drag chain 800 overlaps with the moving space of the operating platform module 600, without adding additional space, and the size of the equipment is compact; by adding a laminar air hood 1300, a laminar air sweeping layer is formed in the upper area of ​​the reagent box 610 to avoid sealing Under the action of mixing and aspirating liquid by the extraction gun tip 1010, the minute gas exchange inside and outside the closed reagent box 610 will lead to cross-contamination of sample aerosols, and the overflowing aerosol will be adsorbed by the second filter 1340 to avoid aerosol residual contamination and aerosol discharge; through the setting of the connecting piece 900, it is small and compact, easy to operate, and will not cause occasional vibration and abnormal noise during the operation of the equipment; through the reasonable layout and design of the rack module 200 and the operating platform module 600, the equipment is streamlined and compact in size, realizing the miniaturization of the all-in-one machine.

[0093] It should be noted that, in this specification, relational terms such as first and second are merely used to distinguish one entity from other entities, but do not necessarily require or imply any actual relationship or order between these entities.

[0094] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0095] The principles and implementation methods of the present invention are described herein using specific examples. The description of the above examples is only intended to help understand the method and core concept of the present invention. It should be noted that those skilled in the art may make various improvements and modifications to the present invention without departing from the principles of the present invention, and such improvements and modifications also fall within the scope of protection of the present invention.

Claims

1. A nucleic acid detection device, characterized in that: include: An xy platform module, comprising a base plate and a first guide rail in the x-direction and a second guide rail in the y-direction located above the base plate; The rack module includes a base, side panels, a power supply, and a main control panel. The side panels are detachably connected to the bottom panel. The optical module, the capping module, the extraction module, and the pipetting module are detachably connected to the base. An operating platform module is located above the xy platform module and is movably connected to the first guide rail and the second guide rail. The operating platform module is provided with a reagent kit for molecular extraction, a microfluidic chip for gene amplification, a temperature control module for PCR reaction, and a drive control board. The drive control board can control the temperature of the temperature control module. The main control board is used to control the operation platform module to move below the optical module, the capping module, the extraction module, and the pipetting module.

2. The nucleic acid detection device according to claim 1, characterized in that The operating platform module also includes: The gun tip seat is arranged between the reagent box and the chip, and the pipetting module transfers the sample liquid on the reagent box to the chip through the gun tip in the gun tip seat.

3. The nucleic acid detection device according to claim 2, characterized in that The temperature control module includes a cooling fin, and the cooling fin can exchange heat with the chip.

4. The nucleic acid detection device according to claim 3, characterized in that A cooling fan is provided at the bottom of the cooling fin, and the cooling fan is used to circulate air to the cooling fin to cool the cooling fin. An exhaust channel for air discharge is provided on one side of the cooling fin, and the exhaust channel includes a first section extending to the xy platform module and a second section arranged at an angle to the first section to allow air to circulate to the outside of the xy platform module.

5. The nucleic acid detection device according to claim 4, characterized in that A first opening is provided on a side of the refrigeration fin facing away from the exhaust passage, and a second opening corresponding to the first opening is provided on the side plate.

6. The nucleic acid detection device according to any one of claims 1 to 5, characterized in that: Also included is a purge module, the purge module comprising: a drive assembly, comprising an air inlet fan and an air outlet fan connected to the base and located above the base; A laminar flow hood, comprising a first air duct having a first air cavity and a second air duct having a second air cavity, wherein one end of each of the first air duct and the second air duct is connected to the bottom of the base, and an air inlet of the first air cavity corresponds to the air inlet fan, and an air outlet of the second air cavity corresponds to the air outlet fan, the air outlet of the first air cavity is located at the other end of the first air duct, and the air inlet of the second air cavity is arranged opposite to the air outlet of the first air cavity; The filter assembly includes a first filter and a second filter, the first filter is arranged at the air outlet of the first air cavity, and the second filter is arranged at the air inlet of the second air cavity, so that a laminar wind blowing layer is formed between the air outlet of the first air cavity and the air inlet of the second air cavity, and the laminar wind blowing layer is located below the extraction module and the pipetting module.

7. The nucleic acid detection device according to any one of claims 1 to 5, characterized in that: It also includes a shell arranged on the outside of the rack module, the shell is provided with a door for the operating platform module to enter and exit the interior of the rack module, and the door is rotatably connected to the shell through an opening and closing mechanism.

8. The nucleic acid detection device according to claim 7, characterized in that The opening and closing mechanism comprises: a fixing member connected to the door; A hinge plate is provided on the housing and is located below the door; An elastic member has one end connected to the fixing member and the other end connected to the hinge plate, and the elastic member is used for automatically closing the door.

9. The nucleic acid detection device according to claim 7, characterized in that The operating platform module is provided with a first roller and a second roller at different heights. The first roller and the second roller can successively abut against the warehouse door to drive the warehouse door to rotate downward.

10. The nucleic acid detection device according to any one of claims 1 to 5, characterized in that: The operating platform module is provided with a first fixed plate, and the rack module is provided with a second fixed plate at the same height as the first fixed plate. The two ends of the drag chain are respectively fixed to the first fixed plate and the second fixed plate to enable the wires of the operating platform module to be transferred to the power supply and main control board of the rack module.

11. The nucleic acid detection device according to any one of claims 1 to 5, characterized in that: A first threaded hole is provided on the side panel, and a second threaded hole corresponding to the first threaded hole is provided on the support seat of the operating platform module. The connecting piece is movably inserted into the first threaded hole, and the connecting piece is sleeved with a spring abutting against the side panel. The connecting piece is provided with a threaded section, and the threaded section can be screwed into the second threaded hole to put the spring in a compressed state.

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