Stem cell culture equipment and use method

Abstract

The invention discloses stem cell culture equipment and a use method, and belongs to the technical field of medical equipment, the stem cell culture equipment comprises an incubator shell, a fixing disc is arranged on one side in the incubator shell, a cavity is formed in the fixing disc, and a shaking assembly is arranged in the cavity. In the device, a driving motor drives a fixed disc, a connecting sleeve and a gear to rotate through a fixed shaft, so that a first roller drives a connecting rod to move upwards, a mounting seat rotates by taking a second spherical hinge support and a spherical hinge support rod as centers, and a culture dish in the mounting seat is slowly inclined and shaken within a set angle; an electric push rod drives a connecting seat, a fixing sleeve, a connecting shaft, a flat plate and a lug to move up and down, and the use height between the lug and a first roller is adjusted, so that the equipment can select a proper inclined shaking angle according to the requirements of the same batch of hair follicle stem cells, and the difference in the same batch is reduced; and different use requirements of the culture dishes in the same batch are effectively ensured.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical equipment, and in particular relates to a stem cell culture device and a method of use. Background Art

[0002] Hair follicle stem cells are a type of stem cell found in the bulge of the outer root sheath of the human hair follicle. Hair follicle stem cells are adult stem cells that are in a dormant state in the body. They exhibit amazing proliferation capacity under in vitro culture. Studies have found that hair follicle stem cells have multidirectional differentiation potential. They can differentiate into the epidermis, hair follicles, and sebaceous glands, and participate in the process of skin wound healing.

[0003] For example, Chinese patent document (CN114181828B) discloses a modular automatic stem cell expansion and culture device, comprising a device body, a culture chamber provided inside the device body, a culture box, an environmental adjustment device, a monitoring component and a liquid adding device provided inside the culture chamber, the environmental adjustment device and the monitoring component being connected to a control device, a plurality of culture modules provided inside the culture box, the liquid adding device being used to add stem cell culture fluid to the culture modules, the culture box being mounted on a mounting seat, sliders being symmetrically provided on the left and right sides of the lower end of the mounting seat, the sliders being slidably connected to slide rails, and the slide rails being arranged at the bottom of the culture chamber, thereby solving the problem that the existing stem cell culture devices on the market do not have the ability to autonomously regulate the cell culture environment. However, during use, the device requires slowly shaking the culture dish during the digestion process to ensure that the hair follicle cells inside the culture dish are fully in contact with the digestive fluid. It is inconvenient to adjust the shaking angle according to the actual needs of the same batch of cells, and its applicability is poor. Therefore, improvement is needed. Summary of the Invention

[0004] The purpose of the present invention is to solve the problem that the existing technology requires slow shaking of the culture dish during the digestion process to ensure that the hair follicle cells inside the culture dish are fully in contact with the digestive fluid, and it is inconvenient to adjust the shaking angle according to the actual needs of the same batch of cells, resulting in poor applicability. A stem cell culture device and method of use are proposed to solve the problem that the existing technology requires slow shaking of the culture dish during the digestion process to ensure that the hair follicle cells inside the culture dish are fully in contact with the digestive fluid, and it is inconvenient to adjust the shaking angle according to the actual needs of the same batch of cells, and the applicability is poor.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions: A stem cell culture device comprises an incubator housing, a fixed plate disposed on one side of the incubator housing, a cavity defined within the fixed plate, a shaking assembly disposed within the cavity, a mounting seat disposed on the top of the shaking assembly, a plurality of placement slots for culture dishes defined within the mounting seat, and a fixing assembly and an auxiliary liquid supply assembly disposed on the bottom side of the shaking assembly. The shaking assembly includes a plurality of connecting sleeves rotatably connected to the inside of the fixed plate, the connecting sleeves are slidably connected to a connecting shaft through a convex groove opened inside, the top of the connecting shaft is fixedly connected to a flat plate, a protrusion is fixedly connected to one side of the top of the flat plate, a fixing cylinder is provided at the bottom of the connecting shaft, and an adjustment unit is provided inside the fixing cylinder, which drives the connecting shaft to move up and down through the adjustment unit to adjust the inclination angle of the mounting seat.

[0006] As a further description of the above technical solution: The bottom of the fixed plate is connected to a driving motor via a fixed shaft, and the bottom of the driving motor is fixedly connected to the inner wall of the incubator shell through a support base. A robotic arm is provided on one side of the fixed plate, and a culture medium supply unit is provided on one side of the robotic arm. The bottoms of the robotic arm and the culture medium supply unit are both fixedly connected to the top of the incubator shell. A channel is opened on the bottom side of the mounting base, and a sensor is provided inside the robotic arm.

[0007] As a further description of the above technical solution: The connecting sleeve is arranged inside the cavity, and the bottom of the connecting sleeve extends to the outside of the fixed plate and is fixedly connected to a gear. The outer peripheral sides of multiple gears are meshed and connected with the same ring gear. The outer peripheral side of the ring gear is fixedly connected to the inner wall of the incubator shell, and the ring gear is set to an incomplete tooth shape.

[0008] As a further description of the above technical solution: A plurality of first rollers are provided on the top of the flat plate, a connecting rod is fixedly connected to the top of the first roller, a first ball joint support is provided on the top of the connecting rod through a ball joint support, the top of the first ball joint support is fixedly connected to the bottom of the mounting seat, a second ball joint support is fixedly connected to the center of the bottom of the first ball joint support, a ball joint support rod is provided on the bottom side of the second ball joint support, the bottom end of the ball joint support rod is fixedly connected to the top of the fixed plate, a first spring is sleeved on the outer peripheral side of the connecting rod, and both sides of the first spring are respectively fixedly connected to the top of the first roller and the inner wall of the fixed plate.

[0009] As a further description of the above technical solution: The bottom of the fixed cylinder is fixedly connected to a connection box, the outer peripheries of the multiple connection boxes are fixedly connected to the same sealing slip ring, the outer periphery of the sealing slip ring is sleeved with an annular frame, and the outer periphery of the annular frame is fixedly connected to the inner wall of the incubator shell.

[0010] As a further description of the above technical solution: The adjusting unit includes an electric push rod, the bottom of the electric push rod is fixedly connected to the inner wall of the fixed cylinder, and one end of the electric push rod output shaft is fixedly connected to a connecting seat, the connecting seat is slidably connected to the inside of the fixed cylinder, the top of the connecting seat is fixedly connected to a fixing sleeve, the top of the fixing sleeve is rotatably connected to the bottom end of the connecting shaft, the cross-sectional shape of the connecting shaft is set to be convex, and the connecting shaft is set to be a hollow shaft, and the fixing sleeve is connected to the connecting shaft through the opened inner cavity.

[0011] As a further description of the above technical solution: The fixing assembly includes an annular seat, which is fixedly connected to the inside of the incubator shell, an annular protrusion is fixedly connected to the outer peripheral side of the top of the annular seat, and a plurality of second rollers are provided on the outer peripheral side of the annular protrusion. The second rollers are fixedly connected to a fixing rod on the side away from the annular protrusion, and the other end of the fixing rod extends to the inside of the connecting box and is fixedly connected to a sliding block, and the sliding block is slidably connected to the inside of the connecting box, and the sliding block is fixedly connected to the main liquid capsule on the side away from the fixing rod, and the other side of the main liquid capsule is fixedly connected to the inner wall of the connecting box.

[0012] As a further description of the above technical solution: The cam is connected to the top of the mounting plate and the bottom of the mounting plate is connected to the cam via a tube connecting the mounting plate and the bottom of the mounting plate, and the cam is connected to the mounting plate via a tube connecting the mounting plate and the bottom of the mounting plate.

[0013] As a further description of the above technical solution: The auxiliary liquid supply component includes a liquid supply bag, the cross-sectional shape of which is set to be annular, and the two sides of the liquid supply bag are respectively fixedly connected to the top of the electric push rod and the inner wall of the fixed cylinder. One side of the liquid supply bag is connected to a liquid outlet pipe, and the other end of the liquid outlet pipe is connected to the culture medium supply unit.

[0014] A method for using a stem cell culture device comprises the following steps: S1. Select bone marrow or umbilical cord blood from a healthy donor as a source of hair follicle stem cells, isolate and purify the hair follicle stem cells through standardized processing steps, then inoculate the isolated hair follicle stem cells into a hair follicle stem cell culture dish, add a specific culture medium to the hair follicle stem cell culture dish, and then place the hair follicle stem cell culture dish into an external hair follicle stem cell culture storage box. The hair follicle stem cell culture storage box provides a suitable culture environment for the hair follicle stem cells, and then the hair follicle stem cell culture storage box is installed in a suitable location on one side of the interior of the incubator shell; S2. When the culture medium of the hair follicle stem cell culture dish needs to be replaced, the robot arm takes the culture dish from the hair follicle stem cell culture storage box, detects the weight of the culture dish through the sensor inside the robot arm, and then places the multiple culture dishes in the placement slots inside the mounting base in sequence; S3. The drive motor drives the fixed plate, the connecting sleeve, and the gear to rotate clockwise 30 degrees via the fixed shaft, thereby moving the culture dish to be directly below the culture medium supply unit. The culture medium supply unit will provide a fixed amount of culture medium or add specific differentiation factors and compounds to the culture dish to promote the growth or differentiation of hair follicle stem cells. S4. As the fixed plate rotates, the gear meshes with the ring gear, and under the action of the ring gear, the gear, the connecting sleeve, the connecting shaft, the flat plate, and the protrusion rotate, so that the first roller drives the connecting rod to move upward, causing the mounting seat to rotate around the second ball joint support and the ball joint support rod, slowly tilting and shaking the culture dish inside the mounting seat within the set angle; S5. The electric push rod drives the connecting seat and the fixing sleeve to move inside the fixing cylinder, so that the connecting shaft drives the flat plate and the protrusion to move up and down, thereby adjusting the height between the protrusion and the first roller. S6. At the same time, the electric push rod squeezes the liquid supply bag, and the liquid in the liquid supply bag is transported to the inside of the culture medium supply unit through the liquid outlet tube; S7. Before the fixed plate drives the mounting seat and moves to the culture medium supply unit, the annular protrusion will squeeze the second roller, causing the second roller to drive the fixed rod to squeeze the sliding block and the main liquid bag, and the liquid inside the main liquid bag is transported to the auxiliary liquid bag through the infusion tube, fixed sleeve, connecting shaft, pipeline, channel and fixed tube, so that the auxiliary liquid bag expands and drives the fan-shaped plate, fan-shaped block and fan-shaped fixed plate to move, and the outside of the culture dish is automatically fixed by the fan-shaped fixed plate.

[0015] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are: 1. In the present invention, a shaking assembly is provided to enable the driving motor to drive the fixed plate, the connecting sleeve and the gear to rotate through the fixed shaft. At this time, the gear will mesh with the ring gear and drive the gear, the connecting sleeve, the connecting shaft, the flat plate and the protrusion to rotate under the action of the ring gear. During the rotation, the protrusion will squeeze the first roller, causing the first roller to drive the connecting rod to move upward, so that the mounting seat rotates around the second ball joint support and the ball joint support rod, and the culture dish inside the mounting seat is slowly tilted and shaken within a set angle, so that the culture solution inside the culture dish produces periodic fluid movement, which helps to enhance the mixing of the culture solution, reduce local nutrient gradients, and make the hair follicle stem cells inside the culture dish evenly absorb substances. The electric push rod drives the connecting seat, fixing sleeve, connecting shaft, flat plate and protrusion to move up and down, and the use height between the protrusion and the first roller is adjusted to adjust the tilt angle of the mounting seat. When the hair follicle stem cells of the same batch grow faster, the cell density inside the culture dish is higher. The tilt and shaking angle of the mounting seat is increased to help promote the diffusion of metabolic waste, break the surface tension layer of the culture solution, improve gas exchange efficiency, and promote nutrient supply in the edge area. The equipment can select the appropriate tilt and shaking angle according to the needs of the hair follicle stem cells of the same batch, reduce the differences within the same batch, and effectively ensure the different usage needs of the culture dishes within the same batch.

[0016] 2. In the present invention, by setting a fixing component, before the fixed plate drives the mounting seat and moves to the culture medium supply unit, the annular protrusion will transport the liquid inside the main liquid sac to the fixed sleeve and the connecting shaft through the second roller, the fixed rod and the sliding block, so that the auxiliary liquid sac will expand and drive the fan-shaped plate, the fan-shaped block and the fan-shaped fixed plate to move. The outside of the culture dish is automatically fixed by the fan-shaped fixed plate, which reduces the slight displacement of the culture dish, reduces the vibration of the culture dish, reduces the risk of cells falling off due to high-frequency micro-vibration, and makes the shaking angle more precise, further improving the use effect of the equipment.

[0017] 3. In the present invention, through the auxiliary liquid supply component, the electric push rod will transport the liquid inside the liquid supply bag through the liquid outlet tube to the inside of the culture medium supply unit to compensate for the consumption rate of the proliferating cells inside the culture dish to ensure their production nutritional needs. The newly added culture fluid will change the liquid level, reducing mechanical damage to cells with high proliferation rates at the same angle. This device uses a dynamic culture method to improve nutrient distribution and gas exchange efficiency while achieving multi-dimensional protection of hair follicle stem cells, ensuring efficient proliferation and maintenance of stemness. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention; Figure 2 Schematic diagram of the overall three-dimensional structure of the fixed plate in the present invention; Figure 3 This is a schematic diagram of the overall three-dimensional structure of the fixed plate of the present invention from another perspective; Figure 4 For the present invention Figure 3 A local enlarged structural diagram of point A; Figure 5 Schematic diagram of the split three-dimensional structure of the fixed disk and the annular seat in the present invention; Figure 6 A schematic diagram of a partial three-dimensional structure of the shaking assembly of the present invention; Figure 7 For the present invention Figure 6 A schematic diagram of the partially enlarged structure at point B; Figure 8 It is a schematic diagram of the overall three-dimensional structure of the shaking assembly in the present invention; Figure 9 Schematic diagram of the internal three-dimensional structure of the mounting base of the present invention; Figure 10 It is a schematic diagram of the internal three-dimensional structure of the connection box in the present invention.

[0019] Legend: 1. Incubator housing; 2. Culture medium supply unit; 3. Robot arm; 4. Fixed plate; 5. Drive motor; 6. Shaking assembly; 601. Ring gear; 602. Gear; 603. Connecting sleeve; 604. Connecting shaft; 605. Flat plate; 606. Bump; 607. First roller; 608. Connecting rod; 609. First ball joint support; 610. Fixed cylinder; 611. Connecting box; 612. Electric push rod; 613. Connecting seat; 6 14. Fixing sleeve; 615. Annular frame; 7. Fixing assembly; 701. Annular seat; 702. Annular protrusion; 703. Second roller; 704. Fixing rod; 705. Sliding block; 706. Main liquid sac; 707. Infusion tube; 708. Second spring; 709. Auxiliary liquid sac; 710. Fan-shaped plate; 711. Fan-shaped block; 712. Fan-shaped fixing plate; 8. Auxiliary liquid supply assembly; 801. Liquid supply sac; 802. Liquid outlet tube; 9. Mounting seat. DETAILED DESCRIPTION

[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0021] See also Figures 1-10The present invention provides a technical solution: a stem cell culture device, comprising an incubator shell 1, a fixed plate 4 is provided on one side of the incubator shell 1, a cavity is defined inside the fixed plate 4, a shaking component 6 is provided inside the cavity, a mounting seat 9 is provided on the top of the shaking component 6, a plurality of placement slots for culture dishes are defined inside the mounting seat 9, and a fixing component 7 and an auxiliary liquid supply component 8 are provided on the bottom side of the shaking component 6; The shaking assembly 6 includes a plurality of connecting sleeves 603 rotatably connected to the inside of the fixed disk 4, the connecting sleeve 603 is slidably connected to the connecting shaft 604 through a convex groove opened inside, the top of the connecting shaft 604 is fixedly connected to a flat plate 605, and a protrusion 606 is fixedly connected to one side of the top of the flat plate 605. A fixed cylinder 610 is provided at the bottom of the connecting shaft 604, and an adjustment unit is provided inside the fixed cylinder 610. The adjusting unit drives the connecting shaft 604 to move up and down to adjust the inclination angle of the mounting seat 9. The bottom of the fixed disk 4 is connected to the driving motor 5 through a fixed shaft, and the bottom of the driving motor 5 is fixedly connected to the inner wall of the incubator shell 1 through a support seat. The fixed disk 4 A robot arm 3 is provided on one side, and a culture medium supply unit 2 is provided on one side of the robot arm 3. The bottom of the robot arm 3 and the culture medium supply unit 2 are fixedly connected to the top of the incubator shell 1. A channel is provided on the bottom side of the mounting seat 9. A sensor is provided inside the robot arm 3. The connecting sleeve 603 is provided inside the cavity, and the bottom of the connecting sleeve 603 extends to the outside of the fixed plate 4 and is fixedly connected to a gear 602. The outer peripheral sides of multiple gears 602 are meshed with the same ring gear 601. The outer peripheral side of the ring gear 601 is fixedly connected to the inner wall of the incubator shell 1. The ring gear 601 is set to an incomplete tooth shape. A plurality of first rollers 607 are provided on the top of the flat plate 605. The top of the first roller 607 is fixedly connected to a connecting rod 608, and the top of the connecting rod 608 is provided with a first ball joint support 609 through a ball joint support. The top of the first ball joint support 609 is fixedly connected to the bottom of the mounting seat 9, and the center of the bottom of the first ball joint support 609 is fixedly connected to a second ball joint support. A ball joint support rod is provided on the bottom side of the second ball joint support, and the bottom end of the ball joint support rod is fixedly connected to the top of the fixed plate 4. The outer peripheral side of the connecting rod 608 is sleeved with a first spring, and the two sides of the first spring are respectively fixedly connected to the top of the first roller 607 and the inner wall of the fixed plate 4. The bottom of the fixed cylinder 610 is fixedly connected to a connecting box 611, and the outer peripheral sides of multiple connecting boxes 611 are fixedly connected to the same sealing slip ring. An annular frame 615 is sleeved on the outer circumference of the sealing slip ring, and the outer circumference of the annular frame 615 is fixedly connected to the inner wall of the incubator shell 1. The adjustment unit includes an electric push rod 612, and the bottom of the electric push rod 612 is fixedly connected to the inner wall of the fixed cylinder 610, and one end of the output shaft of the electric push rod 612 is fixedly connected to a connecting seat 613, and the connecting seat 613 is slidably connected to the inside of the fixed cylinder 610. A fixing sleeve 614 is fixedly connected to the top of the connecting seat 613, and the top of the fixing sleeve 614 is rotatably connected to the bottom end of the connecting shaft 604. The cross-sectional shape of the connecting shaft 604 is set to be convex, and the connecting shaft 604 is set to be a hollow shaft, and the fixing sleeve 614 is connected to the connecting shaft 604 through the opened inner cavity.

[0022] Specific implementation method: According to actual needs, the hair follicle stem cell culture box is installed at a suitable place on one side of the interior of the incubator shell 1. Then, the hair follicle stem cell culture dish is placed inside the hair follicle stem cell culture box. When the culture medium of the hair follicle stem cell culture dish needs to be replaced, the robot 3 is used to pick up the culture dish inside the hair follicle stem cell culture box. The weight of the culture dish is detected by the sensor inside the robot 3. Then, multiple culture dishes are placed in the placement slot inside the mounting seat 9 in sequence. The drive motor 5 and the electric push rod 612 are started according to actual needs, so that the drive motor 5 passes through the fixed axis to move. The fixed plate 4, the connecting sleeve 603 and the gear 602 are driven to rotate clockwise 30 degrees to move the culture dish to the bottom of the culture medium supply unit 2. The culture medium supply unit 2 will provide a fixed amount of culture medium to the culture dish. As the fixed plate 4 rotates, the gear 602 will mesh with the ring gear 601 and drive the gear 602, the connecting sleeve 603, the connecting shaft 604, the flat plate 605 and the protrusion 606 to rotate under the action of the ring gear 601. The protrusion 606 will squeeze the first roller 607 during the rotation process, so that the first roller 607 drives the connecting rod 60 8 moves upward, causing the mounting seat 9 to rotate around the second ball-joint support and the ball-joint support rod, slowly tilting and shaking the culture dish inside the mounting seat 9 within a set angle, causing the culture fluid inside the culture dish to produce periodic fluid movement, helping to enhance the mixing of the culture fluid, reducing local nutrient gradients, and allowing the hair follicle stem cells inside the culture dish to evenly absorb substances, which is beneficial to the metabolism and growth of the hair follicle stem cells. The electric push rod 612 drives the connecting seat 613 and the fixing sleeve 614 to move inside the fixing cylinder 610, causing the connecting shaft 604 to drive the flat plate 605 and the bump 606 to move up and down. The height between the protrusion 606 and the first roller 607 is adjusted to adjust the tilt angle of the mounting base 9. When hair follicle stem cells from the same batch are growing rapidly, the cell density inside the culture dish is high. Increasing the tilt and shaking angle of the mounting base 9 helps promote the diffusion of metabolic waste, breaks the surface tension layer of the culture fluid, improves gas exchange efficiency, and promotes nutrient supply to the edge area. This allows the device to select the appropriate tilt and shaking angle based on the needs of the hair follicle stem cells from the same batch, reducing differences within the batch and effectively ensuring the different usage requirements of the culture dishes within the same batch.

[0023] The fixing assembly 7 includes an annular seat 701, which is fixedly connected to the inside of the incubator shell 1. An annular protrusion 702 is fixedly connected to the outer peripheral side of the top of the annular seat 701. A plurality of second rollers 703 are provided on the outer peripheral side of the annular protrusion 702. The second rollers 703 are fixedly connected to a fixing rod 704 on the side away from the annular protrusion 702. The other end of the fixing rod 704 extends to the inside of the connecting box 611 and is fixedly connected to a sliding block 705. The sliding block 705 is slidably connected to the inside of the connecting box 611. The sliding block 705 is fixedly connected to the main liquid capsule 706 on the side away from the fixing rod 704. The other side of the main liquid capsule 706 is fixedly connected to the inner wall of the connecting box 611. Second springs 708 are provided on both sides of the main liquid capsule 706. The two sides of the second spring 708 are respectively fixedly connected to one side of the sliding block 705 and the inner wall of the connecting box 611. The top side of the main liquid capsule 706 is connected to the inner wall of the connecting box 611. There is an infusion tube 707, the other end of which is connected to the inner cavity inside the fixed sleeve 614, and the top of the connecting shaft 604 is connected to the channel inside the mounting seat 9 through a pipe. There are four auxiliary liquid capsules 709 in a circular array inside the mounting seat 9, one side of the auxiliary liquid capsule 709 is connected to the channel inside the mounting seat 9 through a fixed tube, one side of the auxiliary liquid capsule 709 is fixedly connected to the inner wall of the mounting seat 9, and the other side of the auxiliary liquid capsule 709 is fixedly connected to a fan-shaped plate 710, and the fan-shaped block 711 is fixedly connected to the side of the fan-shaped plate 710 away from the auxiliary liquid capsule 709. The fan-shaped plate 710 and the fan-shaped block 711 are both slidably connected to the inside of the mounting seat 9, and the fan-shaped block 711 extends to the inside of the placement groove away from the side of the fan-shaped plate 710 and is fixedly connected to a fan-shaped fixing plate 712. A third spring is provided on both sides of the auxiliary liquid capsule 709, and the two sides of the third spring are respectively fixedly connected to one side of the fan-shaped plate 710 and the inner wall of the mounting seat 9.

[0024] Specific implementation: The fixed disk 4 will drive the mounting seat 9 and move to the culture medium supply unit 2. The annular protrusion 702 will squeeze the second roller 703, so that the second roller 703 drives the fixed rod 704 to squeeze the sliding block 705 and the main liquid bag 706, and the liquid inside the main liquid bag 706 is transported to the fixed sleeve 614 and the connecting shaft 604 through the infusion tube 707. Thereafter, it is transported to the inside of the channel inside the mounting seat 9 through the pipeline, and then transported to the inside of the auxiliary liquid bag 709 through the fixed tube, so that the auxiliary liquid bag 709 expands and drives the fan plate 710, the fan block 711 and the fan fixing plate 712 to move. The outside of the culture dish is automatically fixed by the fan fixing plate 712, which reduces the slight displacement of the culture dish, reduces the vibration of the culture dish, reduces the risk of cells falling off due to high-frequency micro-vibration, and makes the shaking angle more precise, further improving the use effect of the equipment. A protective layer can be set inside the fan fixing plate 712 according to actual needs.

[0025] The auxiliary liquid supply component 8 includes a liquid supply bag 801, the cross-sectional shape of the liquid supply bag 801 is set to be annular, and the two sides of the liquid supply bag 801 are respectively fixedly connected to the top of the electric push rod 612 and the inner wall of the fixed cylinder 610. One side of the liquid supply bag 801 is connected to a liquid outlet pipe 802, and the other end of the liquid outlet pipe 802 is connected to the culture medium supply unit 2.

[0026] Specific implementation method: The electric push rod 612 will squeeze the liquid supply bag 801 and transport the liquid inside the liquid supply bag 801 to the inside of the culture medium supply unit 2 through the liquid outlet tube 802 to compensate for the consumption rate of the proliferating cells inside the culture dish to ensure their production nutritional needs. The newly added culture fluid will change the liquid level height, reducing mechanical damage to cells with high proliferation rates at the same angle. This allows the device to achieve multi-dimensional protection of hair follicle stem cells through a dynamic culture method, while improving nutrient distribution and gas exchange efficiency, ensuring efficient proliferation and maintenance of stemness.

[0027] A method for using a stem cell culture device comprises the following steps: S1. Select bone marrow or umbilical cord blood from a healthy donor as a source of hair follicle stem cells, separate and purify the hair follicle stem cells through standardized processing steps, then inoculate the separated hair follicle stem cells into a hair follicle stem cell culture dish, add a specific culture medium to the hair follicle stem cell culture dish, and then place the hair follicle stem cell culture dish into an external hair follicle stem cell culture storage box. The hair follicle stem cell culture storage box provides a suitable culture environment for the hair follicle stem cells, and then the hair follicle stem cell culture storage box is installed in a suitable location on one side of the interior of the incubator housing 1; S2. When the culture medium of the hair follicle stem cell culture dishes needs to be replaced, the robot arm 3 is used to take the culture dishes from the hair follicle stem cell culture storage box. The weight of the culture dishes is detected by the sensor inside the robot arm 3. Then, the culture dishes are sequentially placed into the placement slots inside the mounting base 9. S3. The drive motor 5 drives the fixed plate 4, the connecting sleeve 603, and the gear 602 to rotate clockwise 30 degrees via the fixed shaft, moving the culture dish to directly below the culture medium supply unit 2. The culture medium supply unit 2 provides a fixed amount of culture medium or adds specific differentiation factors and compounds to the culture dish to promote the growth or differentiation of hair follicle stem cells. S4. As the fixed plate 4 rotates, the gear 602 meshes with the ring gear 601. Under the action of the ring gear 601, the gear 602, the connecting sleeve 603, the connecting shaft 604, the flat plate 605 and the protrusion 606 rotate, so that the first roller 607 drives the connecting rod 608 to move upward, causing the mounting seat 9 to rotate around the second ball joint support and the ball joint support rod, and slowly tilting and shaking the culture dish inside the mounting seat 9 within a set angle; S5: The electric push rod 612 drives the connecting seat 613 and the fixing sleeve 614 to move inside the fixing cylinder 610, so that the connecting shaft 604 drives the flat plate 605 and the protrusion 606 to move up and down, thereby adjusting the height between the protrusion 606 and the first roller 607. S6. At the same time, the electric push rod 612 squeezes the liquid supply capsule 801, and delivers the liquid inside the liquid supply capsule 801 to the inside of the culture medium supply unit 2 through the liquid outlet tube 802. S7, before the fixed plate 4 drives the mounting seat 9 and moves to the culture medium supply unit 2, the annular protrusion 702 will squeeze the second roller 703, so that the second roller 703 drives the fixed rod 704 to squeeze the sliding block 705 and the main liquid sac 706, and the liquid inside the main liquid sac 706 is transported to the inside of the auxiliary liquid sac 709 through the infusion tube 707, the fixed sleeve 614, the connecting shaft 604, the pipeline, the channel and the fixed tube, so that the auxiliary liquid sac 709 expands and drives the fan plate 710, the fan block 711 and the fan fixed plate 712 to move, and the outside of the culture dish is automatically fixed by the fan fixed plate 712.

[0028] Working principle: When in use, bone marrow or umbilical cord blood from a healthy donor is selected as the source of hair follicle stem cells, and the hair follicle stem cells are separated and purified through standardized processing steps. The separated hair follicle stem cells are then inoculated into a hair follicle stem cell culture dish, and a specific culture medium is added to the hair follicle stem cell culture dish. The hair follicle stem cell culture dish is then placed into an external hair follicle stem cell culture storage box. The hair follicle stem cell culture storage box provides a suitable culture environment for the hair follicle stem cells, and the hair follicle stem cell culture storage box is then installed in a suitable place on one side of the incubator shell 1. When the culture medium of the hair follicle stem cell culture dish needs to be replaced, the robot 3 is used to take the culture dish inside the hair follicle stem cell culture storage box, and the weight of the culture dish is detected by the sensor inside the robot 3. Then, multiple culture dishes are placed in the placement slot inside the mounting seat 9 in sequence. The drive motor 5 and the electric push rod 612 are started according to actual needs, so that the drive motor 5 drives the fixed plate 4, the connecting sleeve 603 and the gear 602 to rotate clockwise by 30 degrees through the fixed shaft, and the culture dish is moved to the bottom of the culture medium supply unit 2. The culture medium supply unit 2 will provide a fixed amount of culture medium to the culture dish. As the fixed plate 4 rotates, the gear 602 will mesh with the ring gear 601, and under the action of the ring gear 601, the gear 602, the connecting sleeve 603, the connecting shaft 604, the flat plate 605 and the protrusion 606 are driven to rotate. During the rotation, the protrusion 606 squeezes the first roller 607, so that the first roller 607 drives the connecting rod 608 to move upward, so that the mounting seat 9 rotates around the second ball joint support and the ball joint support rod, and the culture dish inside the mounting seat 9 is slowly tilted and shaken within the set angle. The electric push rod 612 drives the connecting seat 613 and the fixing sleeve 614 to move inside the fixing cylinder 610, so that the connecting shaft 604 drives the flat plate 605 and the protrusion 606 to move up and down, and adjusts the use height between the protrusion 606 and the first roller 607 to adjust the inclination angle of the mounting seat 9. At the same time, the electric push rod 612 squeezes the liquid supply capsule 801, and transports the liquid inside the liquid supply capsule 801 to the inside of the culture medium supply unit 2 through the liquid outlet tube 802 to compensate for the consumption rate of the proliferating cells inside the culture dish. Before the fixed plate 4 drives the mounting seat 9 and moves to the culture medium supply unit 2, the annular protrusion 702 will squeeze the second roller 703, so that the second roller 703 drives the fixed rod 704 to squeeze the sliding block 705 and the main liquid sac 706, and the liquid inside the main liquid sac 706 is transported to the fixed sleeve 614 and the connecting shaft 604 through the infusion tube 707, and then transported to the inside of the channel inside the mounting seat 9 through the pipeline, and then transported to the inside of the auxiliary liquid sac 709 through the fixed tube, so that the auxiliary liquid sac 709 expands and drives the fan plate 710, the fan block 711 and the fan fixed plate 712 to move, and the outside of the culture dish is automatically fixed by the fan fixed plate 712, which is convenient to use.

[0029] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A stem cell culture device, comprising an incubator housing (1), characterized in that: A fixed plate (4) is provided on one side of the interior of the incubator shell (1), a cavity is provided in the interior of the fixed plate (4), a shaking assembly (6) is provided in the interior of the cavity, a mounting seat (9) is provided on the top of the shaking assembly (6), a plurality of placement slots for culture dishes are provided in the interior of the mounting seat (9), and a fixed assembly (7) and an auxiliary liquid supply assembly (8) are provided on the bottom side of the interior of the shaking assembly (6); The shaking assembly (6) comprises a plurality of connecting sleeves (603) rotatably connected to the interior of the fixed plate (4); the connecting sleeves (603) are slidably connected to a connecting shaft (604) via a convex groove provided therein; a flat plate (605) is fixedly connected to the top of the connecting shaft (604); a bump (606) is fixedly connected to one side of the top of the flat plate (605); a fixing cylinder (610) is provided at the bottom of the connecting shaft (604); an adjusting unit is provided inside the fixing cylinder (610); and the adjusting unit drives the connecting shaft (604) to move up and down to adjust the inclination angle of the mounting seat (9).

2. The stem cell culture device according to claim 1, characterized in that: The bottom of the fixed plate (4) is connected to a driving motor (5) via a fixed shaft, and the bottom of the driving motor (5) is fixedly connected to the inner wall of the incubator shell (1) via a support base. A robot arm (3) is provided on one side of the fixed plate (4), and a culture medium supply unit (2) is provided on one side of the robot arm (3). The bottoms of the robot arm (3) and the culture medium supply unit (2) are both fixedly connected to the top of the incubator shell (1). A channel is provided on the bottom side of the interior of the mounting base (9), and a sensor is provided inside the robot arm (3).

3. The stem cell culture device according to claim 1, characterized in that: The connecting sleeve (603) is arranged inside the cavity, and the bottom of the connecting sleeve (603) extends to the outside of the fixed disk (4) and is fixedly connected to a gear (602), and the outer peripheral sides of the plurality of gears (602) are meshedly connected to the same ring gear (601), and the outer peripheral side of the ring gear (601) is fixedly connected to the inner wall of the incubator shell (1), and the ring gear (601) is arranged to have an incomplete tooth shape.

4. The stem cell culture device according to claim 1, wherein: A plurality of first rollers (607) are provided on the top of the flat plate (605), and a connecting rod (608) is fixedly connected to the top of the first roller (607), and a first ball joint support (609) is provided on the top of the connecting rod (608) through a ball joint support, and the top of the first ball joint support (609) is fixedly connected to the bottom of the mounting seat (9), and a second ball joint support is fixedly connected to the center of the bottom of the first ball joint support (609), and a ball joint support rod is provided on the bottom side of the second ball joint support, and the bottom end of the ball joint support rod is fixedly connected to the top of the fixed plate (4), and a first spring is sleeved on the outer peripheral side of the connecting rod (608), and the two sides of the first spring are fixedly connected to the top of the first roller (607) and the inner wall of the fixed plate (4) respectively.

5. The stem cell culture device according to claim 2, characterized in that: The bottom of the fixed cylinder (610) is fixedly connected to a connection box (611), and the outer peripheral sides of the plurality of connection boxes (611) are fixedly connected to a same sealing slip ring, and the outer peripheral side of the sealing slip ring is sleeved with an annular frame (615), and the outer peripheral side of the annular frame (615) is fixedly connected to the inner wall of the incubator shell (1).

6. The stem cell culture device according to claim 5, characterized in that: The adjustment unit includes an electric push rod (612), the bottom of the electric push rod (612) is fixedly connected to the inner wall of the fixed cylinder (610), and one end of the output shaft of the electric push rod (612) is fixedly connected to a connecting seat (613), the connecting seat (613) is slidably connected to the inside of the fixed cylinder (610), the top of the connecting seat (613) is fixedly connected to a fixing sleeve (614), the top of the fixing sleeve (614) is rotatably connected to the bottom end of the connecting shaft (604), the cross-sectional shape of the connecting shaft (604) is set to be convex, and the connecting shaft (604) is set to be a hollow shaft, and the fixing sleeve (614) is connected to the connecting shaft (604) through the opened inner cavity.

7. The stem cell culture device according to claim 6, characterized in that: The fixing assembly (7) comprises an annular seat (701), the annular seat (701) being fixedly connected to the interior of the incubator shell (1), an annular protrusion (702) being fixedly connected to the outer peripheral side of the top of the annular seat (701), a plurality of second rollers (703) being provided on the outer peripheral side of the annular protrusion (702), the second rollers (703) being fixedly connected to a fixing rod (704) on the side away from the annular protrusion (702), the other end of the fixing rod (704) extending into the interior of the connection box (611) and fixedly connected to a sliding block (705), the sliding block (705) being slidably connected to the interior of the connection box (611), the side of the sliding block (705) being fixedly connected to a main liquid capsule (706) on the side away from the fixing rod (704), the other side of the main liquid capsule (706) being fixedly connected to the inner wall of the connection box (611).

8. The stem cell culture device according to claim 7, characterized in that: A second spring (708) is provided on both sides of the main liquid capsule (706), and the second spring (708) is fixedly connected to one side of the sliding block (705) and the inner wall of the connecting box (611) on both sides. A liquid infusion tube (707) is connected to one side of the top of the main liquid capsule (706), and the other end of the liquid infusion tube (707) is connected to the inner cavity of the fixed sleeve (614). The top of the connecting shaft (604) is connected to the channel inside the mounting seat (9) through a pipeline. Four auxiliary liquid capsules (709) are arranged in a circumferential array inside the mounting seat (9). One side of the auxiliary liquid capsule (709) is connected to the channel inside the mounting seat (9) through a fixed tube. One side of the capsule (709) is fixedly connected to the inner wall of the mounting seat (9), and the other side of the auxiliary liquid capsule (709) is fixedly connected to a fan-shaped plate (710). The fan-shaped plate (710) is fixedly connected to a fan-shaped block (711) on the side away from the auxiliary liquid capsule (709). The fan-shaped plate (710) and the fan-shaped block (711) are both slidably connected to the inside of the mounting seat (9). The fan-shaped block (711) extends to the inside of the placement groove on the side away from the fan-shaped plate (710) and is fixedly connected to a fan-shaped fixing plate (712). A third spring is provided on both sides of the auxiliary liquid capsule (709), and the two sides of the third spring are respectively fixedly connected to one side of the fan-shaped plate (710) and the inner wall of the mounting seat (9).

9. The stem cell culture device according to claim 6, characterized in that: The auxiliary liquid supply component (8) includes a liquid supply bag (801), the cross-sectional shape of the liquid supply bag (801) is set to be annular, and the two sides of the liquid supply bag (801) are fixedly connected to the top of the electric push rod (612) and the inner wall of the fixed cylinder (610), respectively. One side of the liquid supply bag (801) is connected to a liquid outlet pipe (802), and the other end of the liquid outlet pipe (802) is connected to the culture medium supply unit (2).

10. A method for using a stem cell culture device, characterized in that: A stem cell culture device according to any one of claims 1 to 9, comprising the following steps: S1. Select bone marrow or umbilical cord blood from a healthy donor as a source of hair follicle stem cells, separate and purify the hair follicle stem cells through standardized processing steps, then inoculate the separated hair follicle stem cells into a hair follicle stem cell culture dish, add a specific culture medium into the hair follicle stem cell culture dish, then place the hair follicle stem cell culture dish into an external hair follicle stem cell culture storage box, the hair follicle stem cell culture storage box provides a suitable culture environment for the hair follicle stem cells, and then the hair follicle stem cell culture storage box is installed in a suitable place on one side of the interior of the incubator shell (1); S2. When the culture medium of the hair follicle stem cell culture dish needs to be replaced, the robot arm (3) is used to take the culture dish inside the hair follicle stem cell culture storage box, and the weight of the culture dish is detected by the sensor inside the robot arm (3), and then the multiple culture dishes are placed in the placement slot inside the mounting seat (9) in sequence; S3, the driving motor (5) drives the fixed disk (4), the connecting sleeve (603) and the gear (602) to rotate clockwise by thirty degrees via the fixed shaft, and the culture dish is moved to the position directly below the culture medium supply unit (2). The culture medium supply unit (2) provides a fixed amount of culture fluid or adds specific differentiation factors and compounds to the culture dish to promote the growth or differentiation of hair follicle stem cells; S4. As the fixed disk (4) rotates, the gear (602) meshes with the ring gear (601), and under the action of the ring gear (601), the gear (602), the connecting sleeve (603), the connecting shaft (604), the flat plate (605) and the protrusion (606) rotate, so that the first roller (607) drives the connecting rod (608) to move upward, so that the mounting seat (9) rotates around the second ball joint support and the ball joint support rod, and the culture dish inside the mounting seat (9) is slowly tilted and shaken within a set angle; S5, the electric push rod (612) drives the connecting seat (613) and the fixing sleeve (614) to move inside the fixing cylinder (610), so that the connecting shaft (604) drives the flat plate (605) and the protrusion (606) to move up and down, thereby adjusting the use height between the protrusion (606) and the first roller (607); S6. At the same time, the electric push rod (612) squeezes the liquid supply bag (801), and transports the liquid inside the liquid supply bag (801) to the inside of the culture medium supply unit (2) through the liquid outlet tube (802); S7, before the fixed plate (4) drives the mounting seat (9) and moves to the culture medium supply unit (2), the annular protrusion (702) squeezes the second roller (703), so that the second roller (703) drives the fixed rod (704) to squeeze the sliding block (705) and the main liquid sac (706), and the liquid inside the main liquid sac (706) is transported to the inside of the auxiliary liquid sac (709) through the infusion tube (707), the fixed sleeve (614), the connecting shaft (604), the pipeline, the channel and the fixed tube, so that the auxiliary liquid sac (709) expands and drives the fan plate (710), the fan block (711) and the fan fixed plate (712) to move, and the outside of the culture dish is automatically fixed by the fan fixed plate (712).

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