Cervical cancer stem cell culture device

Abstract

The invention belongs to the technical field of stem cell culture, and particularly relates to a cervical cancer stem cell culture device which comprises an incubator and two placing trays arranged in the incubator, and the diameter of the lower placing tray is larger than that of the upper placing tray; a plurality of groups of clamping parts are arranged on each placing disc in a circumferential array and are used for clamping the culture bottles on the placing disc; the observation box is communicated with the side surface of the incubator, and a sealing part is arranged at the communicated part of the observation box and the incubator; the clamping and transferring part is arranged at the top of the culture box and is used for clamping and transferring the culture bottle placed on the placing disc into the observation box; an operator can directly take out a to-be-inspected culture bottle from the observation box without opening a bin door of the culture box, so that the situation that stem cells which are not qualified after being cultured in the culture box cannot be continuously cultured is avoided, the accuracy and the high efficiency of the culture box on stem cell culture can be improved, and the working efficiency of the culture box is improved. And unnecessary waste is not caused.

Description

Technical Field

[0001] The present invention belongs to the technical field of stem cell culture, and in particular relates to a cervical cancer stem cell culture device. Background Art

[0002] Cervical cancer stem cells are closely related to the occurrence and development of cervical cancer. Cervical cancer stem cells have become one of the hot topics in current tumor research. The primary issue in conducting related research is the cultivation of cervical cancer stem cells.

[0003] The in vitro culture of stem cells has strict requirements on the humidity and temperature of the culture environment. Therefore, it is usually carried out in a cell culture incubator. Most existing incubators can store multiple culture dishes or culture bottles to achieve the cultivation of cervical cancer stem cells. In order to observe the quality and status of the stem cell culture in the incubator, most of them are equipped with an observation window for naked eye observation.

[0004] Obviously, the above method cannot accurately check the quality and status of stem cells cultured in culture dishes or culture bottles. If the door of the incubator is directly opened and one culture dish or culture bottle is taken out for observation, the stem cells in other culture dishes or culture bottles will not be able to continue to be cultured in the incubator, which will cause unnecessary waste of stem cells cultured in multiple culture bottles in the incubator and affect the quality of stem cell culture. Summary of the Invention

[0005] To solve the above technical problems, the present invention is achieved through the following technical solutions:

[0006] The present invention is a cervical cancer stem cell culture device, comprising an incubator and two placement trays placed therein, wherein the diameter of the lower placement tray is larger than the diameter of the upper placement tray; a clamping portion, wherein each placement tray has multiple groups of clamping portions in a circumferential array, for clamping culture bottles located on the placement tray; an observation box, which is connected to the side of the incubator, and a sealing component is provided at the connection between the observation box and the incubator; a clamping transfer portion, which is placed on the top of the incubator and moves within the incubator and the observation box, for clamping and transferring the culture bottles placed on the placement tray to the observation box.

[0007] Furthermore, the clamping part includes an arc-shaped clamping plate, a side plate, a guide block and a spring member, and multiple groups of symmetrical arc-shaped clamping plates are placed in multiple holes opened on the placement plate, and the bottom of the holes are blocked by a mesh plate; the symmetrical side plates are respectively connected to the corresponding ends of each arc-shaped clamping plate, and the symmetrical side plates are located on the upper surface of the placement plate; sliding grooves are opened on the inner sides of the holes, and two guide blocks are slidingly arranged in the sliding grooves, each guide block is connected to each side plate, and a limiting block is arranged in the middle of the sliding groove; one end is connected to the guide block, and the other end is connected to the groove wall of the sliding groove.

[0008] Furthermore, the clamping and transferring part includes a screw member, a square sleeve, an electric push rod, an electric clamp, a clamping plate and a wedge block. The screw member is rotatably installed on the top of the incubator, and its outer end extends to the observation box, and a ball nut pair is sleeved on the screw member; the square sleeve is sleeved on the ball nut pair, and two electric push rods are fixed on its lower surface; the bottom of the output rod of the electric push rod on the right is fixed with an electric clamp, and the clamping claw of the electric clamp is provided with a clamping plate; the bottom of the output rod of the electric push rod on the left is installed with a wedge block.

[0009] Furthermore, the sealing component includes a sealing plate, a sealing strip, a sliding support block and a double-rotation screw. The symmetrical sealing plate is located on the outside of the through square hole opened on the side wall of the incubator; the sealing strip is connected to the upper surface of the sealing plate and is located in the movable square hole opened above the through square hole. The symmetrical seals are located in the movable square hole, and the sides of the two seals that contact each other form an avoidance hole; the two sliding support blocks are respectively connected to the sealing plate, and each sliding support block is installed with a ball nut pair; the double-rotation screw is rotatably installed inside the observation box, and the double-rotation screw is inserted into the two ball nut pairs; support grooves are opened on the outer sides of the through square hole and the movable square hole for sliding support of the sealing plate and the sealing strip.

[0010] Furthermore, a protective layer is formed on the top of the incubator, and a recessed cavity is opened in the protective layer. The placement plate located above is rotatably installed in the incubator through the shaft tube, and the placement plate located below is rotatably installed in the incubator through the rotating tube. The rotating tube is inserted into the shaft tube through the rotation of the bearing, and the top of the shaft tube is extended into the recessed cavity through the rotation of the bearing. The top of the rotating tube also extends upward into the recessed cavity, and the height of the top of the rotating tube is higher than the height of the shaft tube. The top ends of the rotating tube and the shaft tube are provided with a ratchet toggle assembly, which cooperates with the clamping transfer part to realize the independent driving and rotation of the rotating tube and the shaft tube.

[0011] Furthermore, the ratchet toggle assembly includes a ratchet disc, a toothed disc, a suspension support block, an active pawl, a stop pawl, a support shaft, a limit bar and a spring, and the two ratchet discs are respectively mounted on the top of the extended rotating tube and the shaft tube; the toothed disc is sleeved on the outside of the ratchet disc, and the toothed disc moves up and down in the recessed cavity; at least two suspension support blocks are rotatably connected to the annular groove opened on the inner ring surface of the toothed disc, and each suspension support block is connected to the output rod of the electric push rod vertically fixed in the recessed cavity; the active pawl is connected to the electric push rod vertically fixed in the recessed cavity through the hinge block It is movably installed on the inner ring surface of the toothed disc and cooperates with the hinge shaft, and the active pawl cooperates with the ratchet disc; the two stopping pawls are both sleeved on the support shaft vertically arranged in the recessed cavity, and each stopping pawl cooperates with each ratchet disc; limit strips are provided on the upper and lower sides of the stopping pawl, and the two limit strips are fixed on the support shaft, and a spring sheet is provided between the two, and the spring sheet is in contact with the stopping pawl; an engagement reset assembly is provided in the recessed cavity, and the engagement reset assembly is transmission-connected to the toothed disc and the square sleeve.

[0012] Furthermore, the engagement reset assembly includes an engagement rack, a supporting strip, an L-shaped bracket and a guide support rod, the engagement rack is horizontally placed in the recessed cavity, and the vertical height of the engagement rack is greater than the distance between the two ratchet discs, and a limited guide groove is provided on the back of the engagement rack; the supporting strip is fixed to the side wall of the recessed cavity, and the supporting strip is slidably inserted into the limited guide groove; the recessed cavity is provided with a supporting groove near the right groove wall, and the supporting groove is connected to the movable cavity opened horizontally on the left and right, the L-shaped bracket is slidably set in the supporting groove, and its left and right horizontal frames are connected to the engagement rack, and its front and rear horizontal frames extend into the movable cavity and correspond to the end face of the square sleeve; the guide support rod is fixed horizontally in the support groove on the left and right, and its outer end portion extends into the recessed cavity, the guide support rod is slidably inserted into the front and rear horizontal frames of the L-shaped bracket, and a spring member is sleeved on the guide support rod, and the two ends of the spring member are respectively connected to the L-shaped bracket and the bottom of the support groove.

[0013] The present invention has the following beneficial effects:

[0014] 1. The present invention provides a clamping and transferring portion in the incubator. The sliding movement of the square sleeve can drive the lifting clamping plate and the wedge block to descend. The wedge block can open the clamping arc clamping plate, and the symmetrical clamping plates can clamp and remove the culture bottle. Then, the square sleeve drives the clamped and lifted culture bottle to be transferred to the observation box, making it convenient for the operator to remove the culture bottle to be inspected from the observation box without directly opening the incubator door. As a result, unqualified stem cells inside will not be unable to continue to be cultured, thereby improving the accuracy and efficiency of stem cell culture in the incubator and avoiding unnecessary waste.

[0015] 2. The diameter of the lower placement tray of the present invention is larger than that of the upper placement tray, and the clamping portion on the lower placement tray is staggered with the clamping portion on the upper placement tray. This not only increases the number of culture bottles that can be cultured and processed by the incubator, but also does not affect the clamping, removal and transfer of the culture bottles clamped on the upper and lower placement trays by the clamping portion. This makes it easier for operators to remove and inspect culture bottles at different positions in the incubator as needed, thereby improving the quality and efficiency of the culture growth of cervical cancer stem cells in the culture bottles in the incubator.

[0016] 3. The present invention enables the upper and lower placement trays to be rotated independently and intermittently through a ratchet toggle assembly, so that the culture bottles clamped on the placement trays can be rotated to correspond to the symmetrical clamping plates. This not only makes it convenient for operators to take out and inspect culture bottles at different positions on the placement trays individually, but also allows the culture bottles that have been cultured to be clamped and removed from the placement trays in turn after the compartment door is opened, thereby making it convenient for operators to quickly, stably and safely take out the culture bottles that have been cultured for use.

[0017] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0019] Figure 1 It is a schematic diagram of the overall structure disclosed in the present invention;

[0020] Figure 2 This is a schematic diagram of the internal structure of the incubator disclosed in the present invention;

[0021] Figure 3 It is a structural schematic diagram of the clamping transfer portion, the ratchet toggle portion and the engagement reset assembly disclosed in the present invention;

[0022] Figure 4 This is a diagram showing the matching state of the placement plate and the clamping transfer portion disclosed in the present invention;

[0023] Figure 5 A schematic diagram of the coordination of the ratchet toggle assembly and the engagement reset assembly disclosed in the present invention;

[0024] Figure 6 This is a cross-sectional view of the incubator disclosed in the present invention.

[0025] In the figure: 1, incubator; 11, through square hole; 12, movable square hole; 13, support chute;

[0026] 2. Placement plate; 21. Hole; 22. Sliding groove; 23. Axis tube; 24. Rotating tube;

[0027] 3. Clamping part; 31. Arc-shaped clamping plate; 32. Side plate; 33. Guide block; 34. Spring member;

[0028] 4. Observation box;

[0029] 5. Clamping and transfer unit; 51. Screw; 52. Square sleeve; 53. Electric push rod; 54. Electric clamping claw; 55. Clamping plate; 56. Wedge block;

[0030] 6. Sealing components; 61. Sealing plate; 62. Sealing strip; 63. Sliding support block; 64. Double-rotation screw;

[0031] 7. Protective layer; 71. Concave cavity; 72. Support groove; 73. Moving cavity;

[0032] 8. Ratchet toggle assembly; 81. Ratchet disc; 82. Toothed disc; 83. Suspension support block; 84. Active pawl; 85. Stop pawl; 86. Support shaft; 87. Limiting bar; 88. Shrapnel;

[0033] 9. Engagement reset assembly; 91. Engagement rack; 92. Support strip; 93. L-shaped bracket; 94. Guide support rod. DETAILED DESCRIPTION

[0034] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0035] In the description of the present invention, it should be understood that the terms "opening", "upper", "lower", "thickness", "top", "middle", "length", "inside", "around" and the like indicating orientation or positional relationship are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the components or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0036] See also Figures 1-6 As shown, the present invention is a cervical cancer stem cell culture device, comprising an incubator 1 and two placement trays 2 placed therein, wherein the lower placement tray 2 has a larger diameter than the upper placement tray 2; clamping portions 3, each placement tray 2 having multiple groups of clamping portions 3 arranged in a circumferential array, for clamping culture bottles placed on the placement trays 2; an observation box 4 connected to the side of the incubator 1, with a sealing component 6 provided at the connection between the observation box 4 and the incubator 1; a clamping transfer portion 5, placed on the top of the incubator 1 and moving within the incubator 1 and the observation box 4, for clamping and transferring culture bottles placed on the placement trays 2 to the observation box 4;

[0037] Specifically, the present invention provides an observation box 4 on the side of the incubator 1. The door of the incubator 1 is opened, and the culture bottles to be cultured can be placed on the placement tray 2 in sequence. Then, the culture bottles are clamped and fixed by the clamping part 3, and the door is closed. Through the control panel on the incubator 1, the interior of the incubator is adjusted to a suitable temperature, humidity, gas and other environments so that the stem cells in multiple culture bottles can be cultured. When it is necessary to inspect the stem cells cultured in the incubator 1, the internal environment of the observation box 4 can be made the same as the internal environment of the incubator 1, and then the sealing component 6 is controlled to open to connect the observation box 4 and the incubator 1. Then, the clamping transfer part 5 is used to grab and fix one of the culture bottles on the placement tray 2, and then the grabbed culture bottle is moved to the observation box 4, and then the sealing component 6 is driven to work to connect the connected culture bottles. The incubator 1 and the observation box 4 are closed, and then the interior of the observation box 4 is made the same as the external environment, and then the door of the observation box 4 is opened, so that the culture bottle can be taken out from the observation box 4, so that the operator can test the stem cells cultured in the culture bottle. If the cultured stem cells meet the qualified quality, the incubator 1 can be stopped and the cultured stem cells can be taken out and used in turn. If the qualified quality is not met, the incubator 1 can continue to work and adjust the internal environment to continue culturing the stem cells in the multiple culture bottles remaining on the placement tray 2. In this way, the uncultured stem cells inside will not be unable to continue to be cultured due to the opening of the door of the incubator 1, thereby improving the accuracy and efficiency of the stem cell culture in the incubator 1 and preventing unnecessary waste.

[0038] In this embodiment, the clamping portion 3 includes an arc-shaped clamping plate 31, a side plate 32, a guide block 33 and a spring member 34. Multiple groups of symmetrical arc-shaped clamping plates 31 are placed in multiple holes 21 opened on the placement plate 2, and the bottom of the hole 21 is blocked by a mesh plate; the symmetrical side plates 32 are respectively connected to the corresponding ends of each arc-shaped clamping plate 31, and the symmetrical side plates 32 are located on the upper surface of the placement plate 2; a sliding groove 22 is opened on the inner side of the hole 21, and two guide blocks 33 are slidingly provided in the sliding groove 22, each guide block 33 is connected to each side plate 32, and a limiting block is provided in the middle of the sliding groove 22; one end is connected to the guide block 33, and the other end is connected to the groove wall of the sliding groove 22;

[0039] Specifically, the symmetrical side plates 32 located inside the hole 21 are moved to drive the guide blocks 33 to slide toward the groove wall in the sliding groove 22. The spring members 34 are compressed to open the symmetrical arc-shaped clamping plates 31 located inside the hole 21, making it easier to place the culture bottles into the hole 21. Then, the symmetrical side plates 32 are slowly released to allow the symmetrical arc-shaped clamping plates 31 to clamp the culture bottles in the hole 21, preventing the culture bottles from shaking violently on the placement tray 2, thereby improving the stability of stem cell culture. The multiple groups of clamping parts 3 on the lower placement tray 2 are located on the periphery of the upper placement tray 2, so that they will not interfere with the clamping and placement of the culture bottles on the upper and lower placement trays 2.

[0040] In this embodiment, the clamping and transferring portion 5 includes a screw member 51, a square sleeve 52, an electric push rod 53, an electric clamping claw 54, a clamping plate 55 and a wedge block 56. The screw member 51 is rotatably mounted on the top of the incubator 1, and its outer end extends to the observation box 4. A ball nut pair is sleeved on the screw member 51; the square sleeve 52 is sleeved on the ball nut pair, and two electric push rods 53 are fixed on its lower surface; an electric clamping claw 54 is fixed to the bottom of the output rod of the electric push rod 53 on the right, and a clamping plate 55 is provided on the clamping claw of the electric clamping claw 54; a wedge block 56 is installed on the bottom of the output rod of the electric push rod 53 on the left;

[0041] Specifically, when the lead screw 51 rotates under the drive of the servo motor connected to the end, it will drive the two electric push rods 53 on the lower surface to move toward the placement tray 2 through the square sleeve 52. When the clamping plate 55 on the electric clamping jaw 54 corresponds to the culture bottle, the wedge block 56 will correspond to the symmetrical side vertical plate 32, and then the output rod of the electric push rod 53 will extend, and the wedge block 56 and the clamping plate 55 will move downward. The clamping plate 55 will clamp the culture bottle under the drive of the electric clamping jaw 54, and the continued descent of the wedge block 56 will push the symmetrical side vertical plates 32 to slide away from each other, thereby facilitating the opening of the arc clamping plate 31, and the output rod of the electric push rod 53 corresponding to the clamping plate 55 will be retracted, which will clamp the culture bottle stored in the hole 21 upward, and the wedge block 56 will also be separated from between the corresponding side vertical plates 32 under the retraction of the output rod of the corresponding electric push rod 53. , and then the screw member 51 drives the square sleeve 52 to slide, so that it can transfer the clamped culture bottle into the opened observation box 4, and then the clamping plate 55 is opened, so that the culture bottle to be inspected can be placed in the observation box 4, thereby realizing the automatic and stable transfer of the culture bottle placed on the placement tray 2 into the observation box 4 for removal and inspection, and will not affect the normal culture use of other culture bottles on the placement tray 2. When the door of the incubator 1 is opened and the culture bottle is taken out, the clamping and transferring part 5 can also remove the culture bottle from the symmetrical arc-shaped clamping plate 31, thereby facilitating the operator to quickly take out the culture bottle clamped and cultured on the placement tray 2 for use; and because the clamping part 3 on the lower placement tray 2 is located on the outer ring surface, the clamping plate 55 and the wedge block 56 only need to move a small distance to realize the clamping and transfer of the culture bottle clamped on the lower placement tray 2.

[0042] In this embodiment, the sealing component 6 includes a sealing plate 61, a sealing strip 62, a sliding support block 63 and a double-rotating screw 64. The symmetrical sealing plate 61 is located on the outside of the through square hole 11 opened on the side wall of the incubator 1; the sealing strip 62 is connected to the upper surface of the sealing plate 61 and is located in the movable square hole 12 opened above the through square hole 11. The symmetrical sealing strips 62 are located in the movable square hole 12, and the sides of the two seals 62 that contact each other form an avoidance hole; the two sliding support blocks 63 are respectively connected to the sealing plate 61, and each sliding support block 63 is installed with a ball nut pair; the double-rotating screw 64 is rotatably installed inside the observation box 4, and the double-rotating screw 64 is inserted into the two ball nut pairs; a support groove 13 is provided on the outer side of the through square hole 11 and the movable square hole 12 for sliding support of the sealing plate 61 and the sealing strip 62;

[0043] Specifically, the servo motor fixed on the back of the observation box 4 is controlled to work so that it drives the connected double-rotation screw 64 to rotate, and then the symmetrical sliding support block 63 will drive the symmetrical sealing plate 61 and the seal 62 to slide away from each other in the support slide groove 13, so as to facilitate the opening of the through square hole 11 and the sliding square hole, so that the clamped culture bottle can accurately enter the observation box 4; and the reverse driving of the double-rotation screw 64 to rotate causes the symmetrical sealing plate 61 and the seal 62 to slide close to each other, so as to achieve the blocking of the through square hole 11 and the movable square hole 12, avoiding the opening of the hole to facilitate the fitting and blocking of the screw member 51 extending into the observation box 4, so that the blocked observation box 4 and the incubator 1 will not be connected, and thus will not affect the normal operation of the incubator 1.

[0044] In this embodiment, a protective layer 7 is formed on the top of the incubator 1, and a recessed cavity 71 is formed in the protective layer 7. The placement tray 2 located above is rotatably installed in the incubator 1 via the shaft tube 23, and the placement tray 2 located below is rotatably installed in the incubator 1 via the rotating tube 24. The rotating tube 24 is rotatably inserted into the shaft tube 23 via a bearing. The top of the shaft tube 23 is rotatably extended into the recessed cavity 71 via the bearing. The top of the rotating tube 24 also extends upward into the recessed cavity 71, and the top height of the rotating tube 24 is higher than the height of the shaft tube 23. The top ends of the rotating tube 24 and the shaft tube 23 are provided with a ratchet toggle assembly 8. The ratchet toggle assembly 8 cooperates with the clamping transfer portion 5 to achieve independent driving and rotation of the rotating tube 24 and the shaft tube 23.

[0045] Specifically, in order to facilitate the grabbing, transfer, or grabbing, detaching, and removing of culture bottles at different positions on the placement tray 2, the upper and lower placement trays 2 are supported by the shaft tube 23 and the rotating tube 24, and the rotating tube 24 is rotated and inserted into the shaft tube 23 so that the two do not interfere with each other's rotation. When the square sleeve 52 slides, it will push the ratchet toggle assembly 8 to work, so that the rotating tube 24 or the shaft tube 23 rotates intermittently separately, and then the upper and lower placement trays 2 rotate intermittently separately, so that the culture bottles clamped on the placement tray 2 can be intermittently transferred to the corresponding movable cavity 73 opened upward at the bottom of the protective layer 7, so that the sliding clamping plate 55 can clamp, detach, transfer, or clamp and remove the culture bottles at different positions on the placement tray 2.

[0046] In this embodiment, the ratchet toggle assembly 8 includes a ratchet disc 81, a toothed disc 82, a suspension support block 83, an active pawl 84, a stop pawl 85, a support shaft 86, a limit bar 87 and a spring 88. The two ratchet discs 81 are respectively mounted on the top of the extended rotating tube 24 and the shaft tube 23; the toothed disc 82 is sleeved on the outside of the ratchet disc 81, and the toothed disc 82 moves up and down in the recessed cavity 71; at least two suspension support blocks 83 are rotatably connected to the annular groove provided on the inner ring surface of the toothed disc 82, and each suspension support block 83 is connected to the output rod of the electric push rod 53 vertically fixed in the recessed cavity 71; the active pawl 84 is connected to the output rod of the electric push rod 53 vertically fixed in the recessed cavity 71; Through the cooperation of the hinge block and the hinge shaft, it is movably installed on the inner ring surface of the toothed disc 82, and the active pawl 84 cooperates with the ratchet disc 81; the two stop pawls 85 are both sleeved on the support shaft 86 vertically arranged in the recessed cavity 71, and each stop pawl 85 cooperates with each ratchet disc 81; the upper and lower sides of the stop pawl 85 are provided with limit strips 87, and the two limit strips 87 are fixed on the support shaft 86, and a spring piece 88 is provided between them, and the spring piece 88 contacts the stop pawl 85; an engagement reset component 9 is provided in the recessed cavity 71, and the engagement reset component 9 is transmission-connected to the toothed disc 82 and the square sleeve 52;

[0047] Specifically, the output rods of at least two electric push rods 53 vertically arranged in the recessed groove are extended and recovered, and the toothed disc 82 can be driven to slide vertically up and down by the suspension support block 83, so that the active pawl 84 hinged on its inner ring surface can cooperate with the ratchet disc 81 on the shaft tube 23 or the ratchet disc 81 on the rotating tube 24. When the active pawl 84 on the toothed disc 82 cooperates with the ratchet disc 81 on the upper rotating tube 24, the engagement reset assembly 9 works, which can drive the toothed disc 82 to rotate, so that the upper ratchet disc 81 rotates intermittently, thereby driving the lower placement disc 2 to rotate intermittently. At this time, the ratchet disc 81 located on the lower shaft tube 23 is in a limited rotation state under the cooperation of the stop pawl 85 sleeved on the support shaft 86. That is to say, the two sets of stop pawls 85 on the support shaft 86 can not only control the intermittently rotating ratchet disc The wheel disc 81 plays the role of reverse limit, and can also play the role of axial limit for the other ratchet disc 81, to prevent the phenomenon that one of the placement discs 2 rotates while the other placement disc 2 rotates. Since the suspension support block 83 in a fixed state is connected to the toothed disc 82 for mutual rotation, the suspension support block 83, supported by the electric push rod 53, will not interfere with the normal rotation of the toothed disc 82, and can drive the toothed disc 82 to slide up and down, driving the active pawl 84 to cooperate with the upper and lower ratchet discs 81 to realize the independent driving and intermittent rotation of the upper and lower placement discs 2, so that when one of the placement discs 2 rotates intermittently to grasp and detect the culture bottle, the other placement disc 2 is in a stationary state and continues to clamp and support the culture bottle, so that the upper and lower placement discs 2 are in a non-interference state to clamp and remove the culture bottle.

[0048] In this embodiment, the engagement reset assembly 9 includes an engagement rack 91, a support strip 92, an L-shaped bracket 93 and a guide support rod 94. The engagement rack 91 is horizontally placed in the recessed cavity 71, and the vertical height of the engagement rack 91 is greater than the distance between the two ratchet discs 81, and a limited position guide groove is provided on the back of the engagement rack 91; the support strip 92 is fixed to the side wall of the recessed cavity 71, and the support strip 92 is slidably inserted into the limit guide groove; a support groove 72 is provided near the right groove wall of the recessed cavity 71, and the support groove 72 is aligned with the left and right horizontal displacement grooves. The movable cavity 73 is connected, and the L-shaped bracket 93 is slidably arranged in the support groove 72, and its left and right horizontal frames are connected to the meshing rack 91, and its front and rear horizontal frames extend into the movable cavity 73 and correspond to the end surface of the square sleeve 52; the guide support rod 94 is fixed horizontally in the support groove 72 on the left and right, and its outer end extends into the recessed cavity 71, and the guide support rod 94 is slidably inserted into the front and rear horizontal frames of the L-shaped bracket 93, and a spring member 34 is sleeved on the guide support rod 94, and the two ends of the spring member 34 are respectively connected to the L-shaped bracket 93 and the bottom of the support groove 72;

[0049] The gear 82 is rotated by the active pawl 84 so that the ratchet disc 81 on the shaft tube 23 is engaged with the gear 81 on the shaft tube 23. At this time, the sliding gear 82 continues to engage with the lower half of the meshing rack 91. After the square sleeve 52 slides leftward in the moving cavity 73 and contacts the front and rear horizontal frames of the L-shaped bracket 93, it will push the meshing rack 91 to slide in the recessed cavity 71 through the L-shaped bracket 93. The supporting strip 92 will slide against the meshing rack 91 in the limiting guide groove, and the guide support rod 94 in the supporting groove 72 will support the L-shaped bracket 93. The spring member 34 will be stretched, and then the rotation of the gear 82 will drive the ratchet disc 81 to rotate through the active pawl 84, so that the culture bottle on the placing tray 2 corresponds to the opened clamping plate 55, which is convenient for quickly removing the culture bottle from the placing tray 2. When the square sleeve 52 moves to the right and is released from the squeezing of the L-shaped bracket 93, the spring member 34 on the guide support rod 94 pulls the L-shaped bracket 93 to slide to the right in the support groove 72, causing the meshing rack 91 to slide in the opposite direction, thereby facilitating the reverse rotation and reset of the toothed disc 82. Therefore, the square sleeve 52 slides back and forth in the movable cavity 73, and through the cooperation of the L-shaped bracket 93 and the meshing rack 91, it can drive the placement disc 2 to rotate intermittently so that the culture bottle corresponds to the opened clamping plate 55, thereby achieving stable grabbing and taking out of the multiple culture bottles clamped on the placement disc 2; when the toothed disc 82 slides upward and corresponds to the ratchet disc 81 at the top of the rotating tube 24 on the lower placement disc 2, the sliding distance of the square sleeve 52 will be shortened, thereby reducing the distance between the holes 21 opened on the lower placement disc 2, so that the culture bottle clamped on the lower placement disc 2 can also accurately correspond to the symmetrical clamping plates 55 for stable grabbing and taking out.

[0050] Throughout this specification, references to terms such as "one embodiment," "example," or "specific example" indicate that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the present invention. In this specification, schematic representations of these terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0051] The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the present invention to the specific embodiments described. Obviously, many modifications and variations are possible based on the content of this specification. These embodiments are selected and described in detail in this specification to better explain the principles and practical applications of the present invention, thereby enabling those skilled in the art to better understand and utilize the present invention. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A cervical cancer stem cell culture device, characterized in that: The incubator comprises an incubator and two placement trays placed therein, wherein the diameter of the lower placement tray is larger than that of the upper placement tray; Clamping parts: each placement tray is provided with a plurality of clamping parts arranged in a circumferential array for clamping the culture bottles on the placement tray; An observation box is connected to the side of the incubator, and a sealing component is provided at the connection between the observation box and the incubator; The clamping and transferring part is placed on the top of the incubator and moves in the incubator and the observation box to clamp and transfer the culture bottles placed on the placement tray to the observation box.

2. The cervical cancer stem cell culture device according to claim 1, wherein the clamping portion include: Arc-shaped splints, multiple sets of symmetrical arc-shaped splints are placed in multiple holes opened on the placement plate, and the bottoms of the holes are blocked by mesh plates; Side uprights, symmetrical side uprights are respectively connected to corresponding ends of each arc-shaped clamping plate, and the symmetrical side uprights are located on the upper surface of the placement tray; The guide block is located on the inner side of the hole and has a sliding groove, and two guide blocks are slidingly arranged in the sliding groove, each guide block is connected to each side plate, and a limit block is set in the middle of the sliding groove; One end of the spring member is connected to the guide block, and the other end is connected to the groove wall of the sliding groove.

3. The cervical cancer stem cell culture device according to claim 1, characterized in that: The clamping and transferring part includes: A lead screw is rotatably mounted on the top of the incubator, with its outer end extending to the observation box, and a ball nut pair is sleeved on the lead screw; The square sleeve is sleeved on the ball nut pair, and two electric push rods are fixed on its lower surface; An electric clamp is fixed on the bottom of the output rod of the electric push rod on the right side, and a clamping plate is provided on the clamping claw of the electric clamp; A wedge block is installed at the bottom of the output rod of the electric push rod on the left.

4. The cervical cancer stem cell culture device according to claim 1, characterized in that: Sealing components include: Sealing plate: The symmetrical sealing plate is located outside the through square hole opened on the side wall of the incubator; The seal is connected to the upper surface of the sealing plate and is located in a movable square hole opened above the through square hole. The symmetrical seals are located in the movable square hole, and the sides of the two seals that contact each other form an avoidance hole. Sliding support blocks, two sliding support blocks are respectively connected to the sealing plate, and each sliding support block is installed with a ball nut pair; A double-rotation screw is rotatably mounted inside the observation box and is inserted into two ball nut pairs; The supporting slide groove is located on the outer side of the through square hole and the movable square hole, and is used to provide sliding support for the sealing plate and the sealing strip.

5. The cervical cancer stem cell culture device according to claim 3, characterized in that: A protective layer is formed on the top of the incubator, and a recessed cavity is opened in the protective layer. The placement plate located above is rotatably installed in the incubator through the shaft tube, and the placement plate located below is rotatably installed in the incubator through the rotating tube. The rotating tube is inserted into the shaft tube through the rotation of the bearing, and the top of the shaft tube is extended into the recessed cavity through the rotation of the bearing. The top of the rotating tube also extends upward into the recessed cavity, and the height of the top of the rotating tube is higher than the height of the shaft tube. The top ends of the rotating tube and the shaft tube are provided with a ratchet toggle assembly, which cooperates with the clamping transfer part to realize the independent driving and rotation of the rotating tube and the shaft tube.

6. The cervical cancer stem cell culture device according to claim 5, characterized in that: The ratchet toggle assembly includes: Ratchet discs, two ratchet discs are respectively mounted on the top of the extended rotating tube and the shaft tube; The toothed disc is sleeved on the outside of the ratchet disc, and the toothed disc moves up and down in the recessed cavity; Suspension support blocks, at least two of which are rotatably connected to the annular groove formed on the inner surface of the gear disc, and each of which is connected to the output rod of the electric push rod vertically fixed in the recessed cavity; The active pawl is movably mounted on the inner ring surface of the gear disc through the cooperation of the hinge block and the hinge shaft, and the active pawl cooperates with the ratchet disc; A stop pawl, wherein both stop pawls are sleeved on a support shaft vertically arranged in the recessed cavity, and each stop pawl cooperates with each ratchet disc; The spring piece and the upper and lower sides of the stop pawl are provided with limit strips, the two limit strips are fixed on the support shaft, and a spring piece is provided between them, and the spring piece is in contact with the stop pawl; The meshing reset assembly is arranged in the recessed cavity and is transmission-connected with the toothed disc and the square sleeve.

7. The cervical cancer stem cell culture device according to claim 6, characterized in that: The engagement reset assembly includes: The meshing rack is horizontally placed in the recessed cavity, and the vertical height of the meshing rack is greater than the distance between the two ratchet discs, and a limiting guide groove is provided on the back of the meshing rack; The supporting strip is fixed on the side wall of the recessed cavity, and the supporting strip is slidably inserted into the limiting guide groove; The L-shaped bracket has a support groove formed near the right side wall of the recessed cavity, and the support groove is connected to the movable cavity formed horizontally on the left and right sides. The L-shaped bracket is slidably arranged in the support groove, and its left and right horizontal frames are connected to the meshing racks, and its front and rear horizontal frames extend into the movable cavity and correspond to the end surface of the square sleeve. The guide support rod is fixed horizontally in the support groove on the left and right sides, and its outer end extends into the recessed cavity. The guide support rod is slidably inserted into the front and rear horizontal frames of the L-shaped bracket, and a spring member is sleeved on the guide support rod. The two ends of the spring member are respectively connected to the L-shaped bracket and the bottom of the support groove.

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