Bifidobacterium longum subsp. infantis for improving scalp environment and preventing hair loss and postbiotic thereof

Abstract

The application discloses a Bifidobacterium longum subsp. infantis strain for improving scalp environment and preventing hair loss and a postbiotic thereof, and belongs to the technical field of washing and protecting products. The application screens a Bifidobacterium longum subsp. infantis strain, obtains the bacterial body and the postbiotic by culture, and obtains a complex of the postbiotic and purple leaf plum extract. The complex exhibits a synergistic effect in the further preparation of a scalp nutrient obtained, and the specific performance is that the skin water content of a subject is significantly improved, the average number of hair loss of the subject is significantly reduced, and the scalp environment is improved. The application has practical value for the comprehensive utilization of the Bifidobacterium longum subsp. infantis strain and the postbiotic thereof, and also has important value for improving the scalp environment and preventing hair loss.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of hair care products, in particular to a Bifidobacterium longum subsp. infantis for improving scalp environment and preventing hair loss. BACKGROUND

[0002] Maintaining healthy and thick hair is an important way to improve personal temperament. In recent years, people have increasingly realized that the health of hair is closely related to the health of the scalp, and many scalp and hair loss problems can be attributed to poor scalp condition. In addition to androgenetic alopecia, alopecia areata and infectious alopecia, an important cause of hair loss is scalp oil imbalance: as everyone generally knows that excessive sebum excretion on the head can cause seborrheic dermatitis, leading to seborrheic alopecia, but frequent shampooing or even daily shampooing to remove oil can have a counterproductive effect, leading to excessively dry scalp, which is particularly prominent in relatively dry areas. Excessively dry scalp not only can cause scalp keratinocyte shedding to form dandruff, but also can cause damage to hair follicles, leading to hair loss.

[0003] Bifidobacterium longum subsp. infantis Streptococcus salivarius subsp. thermophilus ) is an important intestinal microorganism for the human intestinal tract and is closely related to intestinal health. The bacteria can efficiently utilize human milk oligosaccharides and produce short-chain fatty acids (such as acetic acid), promote the maturation of the intestinal barrier function, inhibit inflammatory reactions, and enhance immune function. Because it can utilize dietary sources and host intestinal carbohydrates to promote host metabolism, it has become an important probiotic supplement.

[0004] Prunus cerasifera Ehrh. Prunus cerasifera 'Atropurpurea' ), also known as red-leaf plum, true red-leaf plum, etc., is a deciduous shrub or small tree, originally from southwest Asia, and is distributed in East China, Central China, North China, Northwest China and Southwest China. It is widely planted in North China and the areas south of it. The fruit of Prunus cerasifera Ehrh. is sweet and can be eaten fresh or processed for consumption. It is rich in vitamin C, dietary fiber and anthocyanins and has antioxidant and digestion-promoting effects.

[0005] Although the efficacy of Bifidobacterium longum subsp. infantis and Prunus cerasifera Ehrh. fruit is known to some extent, the efficacy of adding Bifidobacterium longum subsp. infantis and its postbiotic for improving scalp environment and preventing hair loss has not been known for a long time. SUMMARY

[0006] In view of the above prior art, the purpose of the present application is to provide a Bifidobacterium longum subsp. infantis for improving scalp environment and preventing hair loss.

[0007] To achieve the above-mentioned purpose, the technical scheme adopted by the present application is as follows:

[0008] In a first aspect of the present application, a Bifidobacterium longum subsp. infantis strain for improving scalp environment and preventing hair loss is provided, wherein the Bifidobacterium longum subsp. infantis strain is Bifidobacterium longum subsp. infantis WZA-BI09 (CCTCC NO: M 20241766) deposited in the China Center for Type Culture Collection. Bifidobacterium longum subsp. lnfantis WZA-BI09), deposited in the China Center for Type Culture Collection, with the accession number CCTCC NO: M 20241766.

[0009] In a second aspect of the present application, the Bifidobacterium longum subsp. infantis strain is used in the preparation of a product for improving scalp environment and preventing hair loss, wherein the amount of the Bifidobacterium longum subsp. infantis strain added in the product is greater than 1×10 6 cfu / mL.

[0010] In a third aspect of the present application, a postbiotic of the Bifidobacterium longum subsp. infantis strain is provided, wherein the postbiotic of the Bifidobacterium longum subsp. infantis strain is prepared by the following method:

[0011] The activated Bifidobacterium longum subsp. infantis strain is inoculated into a fermentation medium for fermentation, the fermentation temperature is 20-50℃, and the fermentation time is 5-50h; the fermented product is centrifuged, the supernatant is freeze-dried, and the postbiotic of the Bifidobacterium longum subsp. infantis strain is obtained.

[0012] Further, the fermentation medium comprises the following components: beef extract powder 5-20 parts, peptone 5-20 parts, glucose 10-40 parts, yeast powder 2-10 parts, magnesium sulfate 0.1-0.5 parts, sodium acetate 2-10 parts, triammonium citrate 1-5 parts, dipotassium hydrogen phosphate 1-5 parts, manganese sulfate 0.01-0.1 parts, Tween 80 0.5-2 parts, L-cysteine 1-5 parts, distilled water 800-1200 parts, and pH=5-7.

[0013] In a fourth aspect of the present application, the postbiotic of the Bifidobacterium longum subsp. infantis strain is used in the preparation of a product for improving scalp environment and preventing hair loss, wherein the product comprises a basic nutrient and a nutritional supplement, the basic nutrient contains a moisturizing agent, an oil, and an emulsifier, and the nutritional supplement is a mixture of the postbiotic of the Bifidobacterium longum subsp. infantis strain and a purple leaf plum extract according to a mass ratio of (0.5-5):(0.5-5).

[0014] Further, the purple leaf plum extract is prepared by the following method:

[0015] Take fresh mature purple leaf plum, wash and remove seeds, cut into pieces, dry in an oven at 60 DEG C, crush to 400 mesh, and obtain purple leaf plum powder; extract the purple leaf plum powder in 75% v / v ethanol at room temperature at a mass / volume ratio of 1g:3ml, each time for 9h, and extract for 3 times; combine the extract obtained each time, concentrate under reduced pressure at 60 DEG C, and then dry in an oven at 75 DEG C, and obtain a purple leaf plum extract.

[0016] Further, the mass ratio of the basic nourishing agent and the nutritional supplement is (1-3)g:(97-99)g.

[0017] Further, in the basic nourishing agent, the moisturizing agent, the oil, and the emulsifier account for 5-20%, 3-12%, and 0.5-2% of the total mass percentage of the basic nourishing agent, respectively, and the balance is deionized water.

[0018] Further, the moisturizing agent is glycerol, the oil is squalane, and the emulsifier is glyceryl oleate.

[0019] Advantages of the present application:

[0020] (1) The present application obtains B. longum subsp. infantis through screening, and the bacteria can improve the scalp environment and prevent hair loss.

[0021] (2) The present application ferments B. longum subsp. infantis, and obtains postbiotics of B. longum subsp. infantis by freeze-drying the supernatant, and forms a complex of the postbiotics and a purple leaf plum extract, i.e., a B. longum subsp. infantis postbiotics complex.

[0022] (3) The present application adds the B. longum subsp. infantis postbiotics complex to a basic nourishing agent containing a moisturizing agent, an oil, and an emulsifier, to obtain a scalp nourishing agent, and uses it for a subject head skin experiment. According to the experimental results, the complex group performs best, in the skin water content test, the skin water content of the subject is increased to 48.33 at eight weeks, and the eight-week change rate of the skin water content is 37.6%; in the average number of hair loss test, the average number of hair loss of the subject is reduced to 65 at eight weeks, and the eight-week change rate of the average number of hair loss is 48.4%. The results not only have a significant advantage over the control group, but also have a greater improvement compared with the postbiotics group and the purple leaf plum group alone, showing a synergistic effect. Specifically, the skin water content of the subject is significantly increased, the average number of hair loss of the subject is significantly reduced, and the scalp environment is improved. The present application not only has practical value for the comprehensive utilization of B. longum subsp. infantis and its postbiotics, but also has important value for improving the scalp environment and preventing hair loss. DETAILED DESCRIPTION

[0023] It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the application. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

[0024] In order for those skilled in the art to more clearly understand the technical solutions of the present application, the technical solutions of the present application will be described in detail below in conjunction with specific embodiments.

[0025] The test materials not specifically described in the embodiments of the present application are all conventional test materials in the art and can be purchased through commercial channels.

[0026] The Bifidobacterium longum subsp. infantis used in the present application has the following preservation information:

[0027] Chinese name: Bifidobacterium longum subsp. infantis WZA-BI09;

[0028] Latin name: Bifidobacterium longum subsp. lnfantis WZA-BI09;

[0029] Preservation number: CCTCC NO: M 20241766;

[0030] Preservation date: August 9, 2024;

[0031] Preservation agency: China Center for Type Culture Collection (CCTCC);

[0032] Preservation site: Wuhan University, Wuhan, China.

[0033] Example 1: Screening of Bifidobacterium longum subsp. infantis

[0034] 1. Isolation and preliminary screening of strains:

[0035] 10 g of infant fecal samples were collected, 1 g of each sample was placed into a 50 mL centrifuge tube containing 9 mL of sterile normal saline, shaken for 15 min to mix thoroughly, and then allowed to stand for 25 s. 2 mL of the suspension was taken from each sample and placed in a 80℃ water bath for 10 min. 1 mL of each was diluted by a factor of 10, and the appropriate gradient of the suspension was selected and plated on MRS agar medium containing 0.3% CaCO3 (prepared by the applicant) and incubated at 37℃ in an incubator for 48 h. Single colonies with the morphology of Bifidobacterium colonies were picked and observed under a microscope using Gram staining to observe the morphological characteristics of the bacterial cells. Gram-positive bacteria were selected. The above-mentioned Gram-positive bacteria were repeatedly streaked on solid screening medium, and the strains that still had the morphology of Bifidobacterium colonies after purification were selected as target strains and numbered for preservation.

[0036] The solid screening medium used was prepared as follows: 50 mL of filtered horse serum, 0.05% L-cysteine hydrochloride and 0.05 mg / mL mupirocin were added to each liter of MRS medium, wherein the L-cysteine hydrochloride was sterilized by filtering through a sterile filter, and the mupirocin was added after the medium was autoclaved and cooled to 40-50°C.

[0037] 2. Strain identification:

[0038] The strain was sent to the Henan Provincial Industrial Microbial Strain Engineering Technology Research Center for 16S rDNA gene sequence identification. The sequence is shown in SEQ ID NO: 1. The identification result showed that the strain belongs to Bifidobacterium longum subsp. infantis (Bifidobacterium longum subsp. infantis). Bifidobacterium longum subsp. lnfantis The strain WZA-BI09 was biologically preserved.

[0039] Example 2: Preparation of a probiotic of Bifidobacterium longum subsp. infantis

[0040] The preparation method is as follows:

[0041] (1) The Bifidobacterium longum subsp. infantis stored in Example 1 was activated to obtain a Bifidobacterium longum subsp. infantis activated bacterial solution. The activation temperature was 37°C, and the activation time was 24 h.

[0042] (2) The Bifidobacterium longum subsp. infantis activated bacterial solution obtained in step (1) was inoculated into a fermentation medium at a 3wt% (g / g) inoculation amount to ferment, to obtain a Bifidobacterium longum subsp. infantis fermentation broth. The fermentation temperature was 37°C, the fermentation time was 48 h, and it was anaerobic. The composition of the fermentation medium used is as follows: beef infusion powder: 10.0 g, peptone: 10.0 g, glucose: 20.0 g, yeast powder: 4.0 g, magnesium sulfate: 0.2 g, sodium acetate: 5 g, triammonium citrate: 2.0 g, dipotassium hydrogen phosphate: 2.0 g, manganese sulfate: 0.05 g, Tween 80: 1.0 g, L-cysteine: 2 g, distilled water to 1 L, and pH = 6.2.

[0043] (3) The Bifidobacterium longum subsp. infantis fermentation broth obtained in step (2) was centrifuged at 30°C, and the supernatant was freeze-dried to obtain a probiotic of Bifidobacterium longum subsp. infantis.

[0044] The content of Bifidobacterium longum subsp. infantis in the precipitate was detected by spectrophotometry, and the freeze-dried product was divided into portions according to a concentration of 5 x 10 8 cfu / ml of live bacterial cells, and then water was added to restore the concentration to obtain a Bifidobacterium longum subsp. infantis bacterial solution, which was stored in a refrigerator at -18°C.

[0045] Example 3: Preparation of a probiotic compound of Bifidobacterium longum subsp. infantis

[0046] Preparation of Prunus cerasifera extract

[0047] Fresh mature Prunus cerasifera fruits were taken, and the peels, stems and seeds were removed. The fruits were cut into pieces and dried in an oven at 60°C. The dried fruits were ground to 400 mesh. The powder was extracted with 75% v / v ethanol at room temperature for 9 hours. The extraction was repeated three times. The extracts were combined and concentrated under reduced pressure at 60°C. The concentrate was dried in an oven at 75°C to obtain Prunus cerasifera extract.

[0048] Preparation of the complex

[0049] The postbiotic of Bifidobacterium longum subsp. infantis prepared in Example 2 and the Prunus cerasifera extract prepared in Example 3 were mixed in a mass ratio of 1:1 to obtain the postbiotic complex of Bifidobacterium longum subsp. infantis.

[0050] Example 4: Preparation of a scalp nutrient

[0051] Basic nutrient

[0052] The basic nutrient contained 10% moisturizer, 6% oil, and 1% emulsifier by total mass percentage (g / g), and the balance was deionized water. The moisturizer was glycerol, the oil was squalane, and the emulsifier was glyceryl oleate.

[0053] Scalp nutrient

[0054] The nutrient supplement was added to the basic nutrient in a mass ratio of 2g:98g to obtain the scalp nutrient. The nutrient supplement used was the postbiotic complex of Bifidobacterium longum subsp. infantis prepared in Example 4.

[0055] Example 5: Preparation of a scalp nutrient

[0056] Basic nutrient

[0057] The basic nutrient contained 10% moisturizer, 6% oil, and 1% emulsifier by total mass percentage (g / g), and the balance was deionized water. The moisturizer was glycerol, the oil was squalane, and the emulsifier was glyceryl oleate.

[0058] Scalp nutrient

[0059] The nutrient supplement was added to the basic nutrient in a mass ratio of 1g:99g to obtain the scalp nutrient. The nutrient supplement used was the postbiotic complex of Bifidobacterium longum subsp. infantis prepared in Example 4.

[0060] Example 6: Preparation of a scalp nutrient

[0061] Basic nutrient

[0062] The base nutrient contains 10% of humectant, 6% of oil, 1% of emulsifier, and the rest is deionized water, wherein the humectant is glycerin, the oil is squalane, and the emulsifier is glyceryl oleate.

[0063] The scalp nutrient

[0064] The scalp nutrient is obtained by adding the nutritional supplement to the base nutrient according to a mass ratio of 3g:97g.

[0065] Preparation of the scalp nutrient

[0066] The difference between the present comparative example 1 and example 4 is that the nutritional supplement used is the postbiotic of Bifidobacterium longum subsp. infantis prepared in example 2.

[0067] The base nutrient

[0068] The base nutrient contains 10% of humectant, 6% of oil, 1% of emulsifier, and the rest is deionized water, wherein the humectant is glycerin, the oil is squalane, and the emulsifier is glyceryl oleate.

[0069] The scalp nutrient

[0070] The scalp nutrient is obtained by adding the nutritional supplement to the base nutrient according to a mass ratio of 2g:98g.

[0071] Preparation of the scalp nutrient

[0072] The difference between the present comparative example 2 and example 4 is that the nutritional supplement used is the extract of Prunus cerasifera prepared in example 3.

[0073] The base nutrient

[0074] The base nutrient contains 10% of humectant, 6% of oil, 1% of emulsifier, and the rest is deionized water, wherein the humectant is glycerin, the oil is squalane, and the emulsifier is glyceryl oleate.

[0075] The scalp nutrient

[0076] The scalp nutrient is obtained by adding the nutritional supplement to the base nutrient according to a mass ratio of 2g:98g.

[0077] Preparation of the scalp nutrient

[0078] The difference between the present comparative example 3 and example 4 is that the nutritional supplement used is the solution of Bifidobacterium longum subsp. infantis prepared in example 2.

[0079] base nutrient

[0080] The base nutrient contains 10% of a humectant, 6% of an oil, and 1% of an emulsifier by total mass percentage (g / g), and the balance is deionized water, wherein the humectant is glycerin, the oil is squalane, and the emulsifier is glyceryl oleate.

[0081] scalp nutrient

[0082] The scalp nutrient is obtained by adding the nutritional supplement to the base nutrient at a mass ratio of 2 g:98 g.

[0083] Test example

[0084] Volunteers aged 25-55 years old were selected as subjects, a total of 40 people, randomly divided into 5 groups, 8 people in each group, 4 men and 4 women in each group.

[0085] Test grouping: Each group was named as probiotic group, purple leaf plum group, microorganism group, complex group and control group. The test samples used by each group are as follows: the test sample used by the probiotic group is the scalp nutrient prepared in Comparative Example 1, the test sample used by the purple leaf plum group is the scalp nutrient prepared in Comparative Example 2, the test sample used by the microorganism group is the scalp nutrient prepared in Comparative Example 3, the test sample used by the complex group is the scalp nutrient prepared in Example 4, and the test sample used by the control group is the base nutrient.

[0086] The use method is as follows: 1 time per night, 5 mL each time, and the specified scalp nutrient of this group is applied to the scalp and gently massaged for 3-5 min until absorption. Matters needing attention: During the test period, the number of hair washing of the subjects follows the daily habits of the subjects, at least three times a week, but only the hair shampoo provided (the shampoo does not contain any active ingredients) is used. During the experiment, the subjects do not use any other hair products, do not use drugs and health products that may affect the experimental results, and do not perform other activities that may interfere with the experimental results. The subjects do not wash their hair within 48 hours before each statistical test, and do not comb their hair on their own on the day of the statistical test.

[0087] The specific statistical test method is as follows:

[0088] Before each test, the subjects sit in a room with a temperature of 20°C and a relative humidity of 50% RH for 30 min.

[0089] For the average number of hair loss test, the technician uses a comb of the same specification (moderate comb tooth density, 1 mm between teeth, comb tooth length of 2.0-3.0 cm, comb length of 10 cm), and combs at a uniform speed for 60 times (30 times on the left and 30 times on the right) in the order of from left front to left back and from right front to right back. The fallen hair is collected and counted.

[0090] For skin moisture content test, at the first test, the water content of the scalp stratum corneum in the head fixed test area was tested by the skin moisture tester Corneometer CM825 before and after application (30 min, 1 h), three times were determined and the average was taken to obtain the moisture content data; the area was tested every time thereafter. The subjects continued to use for eight weeks, and the skin test was carried out at the fourth week and the eighth week, respectively. The skin moisture content test results are shown in Table 1.

[0091] Table 1 Skin moisture content

[0092]

[0093] As can be seen from Table 1, compared with the control group, the skin moisture content of the subjects in each experimental group after eight weeks was significantly improved. Among them, the compound group performed best, and in the compound group, the skin moisture content of the subjects was 48.33 after eight weeks, and the eight-week change rate of the skin moisture content was 37.6%. It can be seen that by adding the long bifidobacterium infantis postbiotic compound prepared in Example 4 to the basic nourishing agent, the moisturizing effect of the scalp nourishing agent is improved, which is more helpful to improve the moisturizing performance of the scalp, and after the compound group is treated, the skin moisture content of the subjects is significantly improved, and the scalp environment is improved.

[0094] The test results of the average number of hair loss are shown in Table 2.

[0095] Table 2 Average number of hair loss (root)

[0096]

[0097] As can be seen from Table 2, compared with the control group, the average number of hair loss of the subjects in each experimental group after eight weeks was significantly decreased. Among them, the compound group performed best, and in the compound group, the average number of hair loss of the subjects was 65 after eight weeks, and the eight-week change rate of the average number of hair loss was 48.4%. It can be seen that by adding the long bifidobacterium infantis postbiotic compound prepared in Example 4 to the basic nourishing agent, the hair loss prevention effect of the scalp nourishing agent is improved, and the scalp environment is improved after the compound group is treated.

[0098] In summary, the complex group showed the best performance. In the skin moisture content test, the skin moisture content of the subjects increased to 48.33 at eight weeks, and the eight-week change rate of skin moisture content was 37.6%. In the average number of hair loss test, the average number of hair loss of the subjects decreased to 65 at eight weeks, and the eight-week change rate of the average number of hair loss was 48.4%. This result not only has a significant advantage over the control group, but also has a greater improvement compared to the use of metagenome group and purple leaf plum group alone, showing a synergistic effect. Specifically, the skin moisture content of the subjects increased significantly, the average number of hair loss of the subjects decreased significantly, and the scalp environment was improved.

[0099] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. Those skilled in the art can make various modifications and changes to the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A strain of Bifidobacterium longum subsp. infantis for improving the scalp environment and preventing hair loss, characterized in that, The B. longum infantis is B. longum infantis WZA-BI09 Bifidobacterium longum subsp. lnfantis WZA-BI09, which is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20241766.

2. Use of B. longum subsp. infantis of claim 1 for the preparation of a product for improving the scalp environment, preventing hair loss, characterized in that, In the product, the added amount of Bifidobacterium longum subsp. infantis is greater than 1 x 10 6 cfu / mL.

3. A postbiotic of Bifidobacterium longum subsp. infantis, characterized in that, The postbiotic of the Bifidobacterium longum subsp. infantis is prepared by the following method: The Bifidobacterium longum subsp. infantis in claim 1 is activated and inoculated into a fermentation medium for fermentation, the fermentation temperature is 20-50℃, and the fermentation time is 5-50h; the fermented product is centrifuged, the supernatant is freeze-dried to obtain the postbiotic of the Bifidobacterium longum subsp. infantis.

4. The postbiotic of Bifidobacterium longum subsp. infantis of claim 3, characterized in that, The fermentation medium is composed of beef extract powder 5-20 parts, peptone 5-20 parts, glucose 10-40 parts, yeast powder 2-10 parts, magnesium sulfate 0.1-0.5 parts, sodium acetate 2-10 parts, triammonium citrate 1-5 parts, dipotassium hydrogen phosphate 1-5 parts, manganese sulfate 0.01-0.1 parts, Tween 80 0.5-2 parts, L-cysteine 1-5 parts, and distilled water 800-1200 parts, pH=5-7.

5. Use of a postbiotic of Bifidobacterium longum subsp. infantis according to claim 3 or 4 for the manufacture of a product for improving the scalp environment, preventing hair loss, characterized in that, The product comprises a basic nutrient and a nutritional supplement, the basic nutrient contains a humectant, an oil and an emulsifier, and the nutritional supplement is a mixture of the postbiotic of the Bifidobacterium longum subsp. infantis and a Prunus cerasus L. extract in a mass ratio of (0.5-5):(0.5-5); the Prunus cerasus L. extract is prepared by the following method: Fresh mature Prunus cerasus L. is washed, deseeded, cut into pieces, dried in an oven at 60℃, and ground to 400 mesh to obtain Prunus cerasus L. powder; the Prunus cerasus L. powder is extracted in 75% v / v ethanol at room temperature at a mass / volume ratio of 1g:3ml, each extraction time is 9h, and the extraction is performed for 3 times; the obtained extraction liquid is combined, concentrated under reduced pressure at 60℃, and then dried in an oven at 75℃ to obtain the Prunus cerasus L. extract.

6. Use of a postbiotic of Bifidobacterium longum subsp. infantis according to claim 5 for the manufacture of a product for improving the scalp environment, preventing hair loss, characterized in that, The mass ratio of the basic nutrient and the nutritional supplement is (1-3)g:(97-99)g.

7. Use of a postbiotic of Bifidobacterium longum subsp. infantis according to claim 6 for the manufacture of a product for improving the scalp environment, preventing hair loss, characterized in that, In the basic nutrient, the humectant, the oil and the emulsifier account for 5-20%, 3-12% and 0.5-2% of the total mass percentage of the basic nutrient respectively, and the balance is deionized water.

8. Use of a postbiotic of Bifidobacterium longum subsp. infantis according to claim 7 for the manufacture of a product for improving the scalp environment, preventing hair loss, characterized in that, The humectant is glycerol, the oil is squalane, and the emulsifier is glyceryl oleate.

Images