Capsicum growth regulator based on compounding of propionyl brassinolide and amino-oligosaccharin and application of capsicum growth regulator

Abstract

The invention discloses a special growth regulator for pepper based on compounding of propionyl brassinolide and amino-oligosaccharin and application of the special growth regulator, and belongs to the technical field of agricultural biology. According to the regulating agent, 0.0008 to 0.0012 percent of propionyl brassinolide and 2.95 to 3.05 percent of amino-oligosaccharin are accurately matched with each other. The formula breaks through the contradiction between the root promotion requirement of high-dose amino-oligosaccharin and the safety threshold of low-dose brassinolide, and overcomes the phytotoxicity risk of the traditional dosage form. The regulator prepared by the invention can enhance the growth vigor of the pepper, improve the product and quality of the pepper, and also can effectively prevent viral diseases of the pepper.

Description

Technical Field

[0001] The invention relates to the field of agricultural biotechnology, and in particular to a plant growth regulator compound composition, which is particularly suitable for regulating the growth and development of peppers and preventing viral diseases. Background Art

[0002] Global pepper cultivation covers over 3.9 million hectares, with China accounting for 54.7% of this, generating an annual output value exceeding 280 billion yuan. High temperatures (>35°C) or low temperatures (<15°C) during the flowering period can cause 30-50% of pepper flowers and fruits to drop, resulting in direct economic losses exceeding 12 billion yuan per year. Deformed fruits account for 12-18% of pepper production, with the percentage of high-quality fruit dropping below 65%. Pepper viral diseases are also a significant concern in production, with no effective control methods available, relying solely on prevention to reduce their incidence. To address these issues, pepper production relies heavily on the use of plant growth regulators to enhance growth and disease resistance.

[0003] The plant growth regulators propionylbrassinolide and amino oligosaccharides have attracted much attention due to their high efficacy and low toxicity. However, single agents have significant limitations in practical application. The application of brassinolide on peppers exhibits a typical "double-edged sword effect." While low concentrations (<0.001 ppm) can promote cell elongation by activating the brassinosteroid signaling pathway, their induction of key photosynthate transport genes is insufficient, resulting in limited yield increases. At concentrations ≥0.003 ppm, endogenous hormone balance is disrupted, leading to disrupted indoleacetic acid and gibberellin synthesis and a surge in the rate of malformed fruit. Aminooligosaccharides enhance root development by inducing chitinase activity. However, the root-promoting effect of amino oligosaccharides used alone on seedlings is unstable and easily affected by environmental conditions.

[0004] Although existing patents (such as CN109169666A) combine propionyl brassinolide and amino oligosaccharides, their focus on viral disease prevention and control and the wide ratio (1:50-500) fail to address the key contradiction in growth regulation. There is an urgent need to develop a growth regulator specifically for peppers to overcome the contradiction between the high doses of active ingredients required to promote flowering and fruiting and the low physiological tolerance of peppers. Summary of the Invention

[0005] In response to the above problems, the present application provides a pepper growth regulator that is a combination of propionyl brassinolide and amino oligosaccharides.

[0006] To achieve the above objectives, this application provides the following technical solutions: The invention provides a pesticide composition containing propionylbrassinolide and aminooligosaccharide. The pesticide composition comprises propionylbrassinolide and aminooligosaccharide, wherein the propionylbrassinolide accounts for 0.0008-0.0012% and the aminooligosaccharide accounts for 2.95-3.05%.

[0007] In the technical solution of the present invention, when the composition is a soluble solution, it is made of the following components by weight percentage: 0.001% propionyl brassinolide, 2.999% amino oligosaccharide, 30% propylene glycol, 0.10% sodium citrate, 0.02% ethoxyquinoline, and deionized water to make up the balance.

[0008] The beneficial effects of the present invention are: The invention provides a pesticide combination containing propionyl brassinolide and amino oligosaccharides. The composition regulates the growth of peppers, protects flowers and fruits, and increases pepper yield and commercial value. The two main components of the invention are both biological pesticides. The resulting compound preparation has no environmental risks, can improve the disease resistance of peppers, especially has a significant effect against viral diseases, and can also enhance the stress resistance of peppers.

[0009] The propionyl brassinolide and amino oligosaccharide in the pesticide composition of the present invention are both biological pesticides. The resulting composite preparation has no environmental risk, is safe and environmentally friendly, does not cause environmental pollution, and can ensure the quality and safety of agricultural products. DETAILED DESCRIPTION

[0010] The technical solution of the present invention is further illustrated below through specific implementation methods. The present invention uses a compound of propionyl brassinolide and amino oligosaccharides to regulate the growth of peppers, promote flowering and fruiting, improve pepper yield and quality, and prevent and treat various pepper diseases, such as viral diseases. Compared with other formulas, the dosage of medicine can be reduced, and the cost-effectiveness is high.

[0011] The following specific application cases can prove this.

[0012] Example 1: 0.001% Propionylbrassinolide·Aminooligosaccharide Soluble Agent The relationship between pepper fruit setting rate and components was established by response surface analysis, in which the concentration of propionyl brassinolide (10⁻ 4 Factor A (%) was used, with levels of 0.5, 0.8, 1.0, 1.2, and 1.5. Factor B (%) was used, with levels of 2.50, 2.75, 2.99, 3.20, and 3.50. A two-factor, five-level design was used, with 13 experimental groups. Foliar sprays were applied at a 1000-fold dilution at the pepper's three-leaf stage. Fruit set was monitored during the fruiting period, and the fruit set rate was calculated using the following formula.

[0013] Fruit setting rate (%) = (number of fruits per plant / total number of flowers) × 100% The model was constructed based on the fruit setting rate and the concentrations of amino oligosaccharides and propionyl brassinolide: Y = 89.24 + 12.35A - 15.47A² + 8.63B - 6.94B² Where: Y: fruit setting rate (%) A: concentration of propionyl brassinolide (×10⁻ 4 %) B: Aminooligosaccharin concentration (%) Verification experiments confirmed that the optimal ratio is A=0.001%, B=2.999% (R²=0.98) The formula is: 0.001% propionyl brassinolide + 2.999% amino oligosaccharide + 30% propylene glycol + 0.10% sodium citrate + 0.02% ethoxyquinoline, with deionized water making up to 100%. The solvent system is propylene glycol, and the stabilizers are sodium citrate and ethoxyquinoline.

[0014] The specific preparation method includes: adding sodium citrate to propylene glycol at 40±2°C and dissolving it; adding ethoxyquinoline and stirring at the same temperature for 10 minutes; cooling to 25°C and adding amino oligosaccharides, stirring and dispersing; adding propionyl brassinolide and continuing to stir; homogenizing under a high pressure of 25MPa; aging at 25°C for 48 hours; and filling with nitrogen after 0.22μm microfiltration.

[0015] Application Example 1: Growth Regulation of Peppers by Propionyl Brassinolide and Oligosaccharides For the test crop pepper, the above-prepared composition was sprayed on the leaves 3 days after transplanting the pepper, during the initial flowering period, and after the first picking. The dosage of each treatment in each embodiment was consistent. The occurrence of phytotoxicity was observed 3 days after each application. 30 days after the last application, 10 plants were randomly selected in each plot to investigate plant height and fruit number, and the number of straight peppers and curved peppers was counted.

[0016] Table 1 Effects of propionyl brassinolide•amino oligosaccharide soluble agent on pepper growth and yield As shown in Table 1, a 1000-fold dilution of propionylbrassinolide and aminooligosaccharides resulted in healthy pepper growth, yield, and quality. The combination of propionylbrassinolide and aminooligosaccharides not only regulates pepper growth and increases yield, but also improves quality.

[0017] Application Example 2: Effects of Propionyl Brassinolide and Oligosaccharides on Pepper Virus Disease For the test crop, peppers were sprayed with the compositions prepared above, beginning at the early stages of pepper virus infestation. Three days after application, the presence of phytotoxicity was observed. Thirty days after the first application, 10 plants were randomly selected from each plot to investigate the occurrence of viral disease. The disease index and control efficacy were calculated as follows.

[0018] The grading standards for pepper virus disease survey are as follows: Level 0, no symptoms; Level 1, obvious or slight mosaic of the heart leaves; Level 3, mosaic of the heart leaves and middle leaves, sometimes with necrotic spots on the leaves; Level 5, most leaves are mosaic, a few leaves are deformed and wrinkled, sometimes with necrotic spots on leaves and stems, or short streaks on the stems; Level 7, most leaves are deformed and slender, or the stems and veins produce systemic necrosis, and the plants are dwarfed; Level 9, the plants have severe systemic mosaic and deformity, or sometimes severe systemic necrosis, the plants are obviously dwarfed, or even die.

[0019] Calculation method for viral disease prevention and control effects:

[0020] Table 2 The control effect of propionyl brassinolide and amino oligosaccharide on pepper virus disease Dilution multiple Disease index Prevention effect% 500 23.6 48.1 1000 15.8 65.3 1500 20.2 55.7 2000 24.9 45.2 comparison 45.5 - As can be seen from Table 2, the 1000-fold solution of propionyl brassinolide•amino oligosaccharide has the best control effect on pepper virus disease.

[0021] The pesticide combination provided by the present invention contains propionyl brassinolide and amino oligosaccharide. Both main components of the present invention are biopesticides. The resulting composite preparation has no environmental risks and can enhance the growth of peppers, increase pepper yield, and improve pepper quality. The propionyl brassinolide and amino oligosaccharide in the pesticide composition of the present invention are both compound preparations formed by biological pesticides, have no environmental risks, are safe and environmentally friendly, will not cause environmental pollution, and can ensure the quality safety of agricultural products.

[0022] The present invention is described through preferred embodiments. Those skilled in the art will appreciate that various modifications or equivalent substitutions may be made to these features and embodiments without departing from the spirit and scope of the present invention. The present invention is not limited to the specific embodiments disclosed herein; other embodiments falling within the scope of the claims of this application are intended to be protected by the present invention.

Claims

1. It is characterized in that The invention comprises the following components in percentage by weight: 0.0008-0.0012% of propionyl brassinolide, 2.95-3.05% of amino oligosaccharide, 28-32% of propylene glycol, 0.08-0.12% of sodium citrate, 0.01-0.03% of ethoxyquinoline, and the remainder is deionized water.

2. The pepper growth regulator according to claim 1, wherein: The content of the propionyl brassinolide is 0.001%, and the content of the amino oligosaccharide is 2.999%.

3. The method for preparing the pepper growth regulator according to claim 1 or 2, comprising: Dissolve sodium citrate in propylene glycol at 40±2℃; Add ethoxyquin and maintain the temperature with stirring for 10 minutes; cool to 25°C, add amino oligosaccharides, and stir to disperse; add propionyl brassinolide and continue stirring; homogenize under 25 MPa high pressure; mature at 25°C for 48 hours; filter through 0.22 μm micropores, and then fill with nitrogen.

4. The method for applying the pepper growth regulator according to any one of claims 1 to 3, characterized in that: Spray the pepper seedlings on the leaves when they are three leaves in the stage of three leaves, using a 1000-fold diluted solution.

5. Use of the pepper growth regulator according to any one of claims 1 to 3 in increasing the fruit setting rate of pepper, reducing the rate of deformed fruit, and enhancing the resistance of pepper to viral diseases.

Images