A system for breeding an anti-bacterial wilt corn plant and a breeding method thereof

Abstract

The application relates to a cultivation system and a cultivation method of a corn plant resistant to bacterial wilt, and belongs to the technical field of plant cultivation.The cultivation system comprises a box, an illumination mechanism and an observation mechanism, multiple independent spaces are arranged in the box, a culture module for cultivating a plant is arranged in each independent space, the illumination mechanism comprises an LED unit matrix plate and a contour scanning assembly, the LED unit matrix plate is arranged at the upper end face of the internal space of the culture module, the contour scanning assembly is used for acquiring a top view contour map of a plant canopy, the observation mechanism comprises a transparent culture plate for bearing the plant and a camera assembly, the transparent culture plate is a hollow structure, the root of the plant is located in the transparent culture plate, and the camera assembly is used for photographing and recording the root of the plant in the transparent culture plate.The application can guarantee that each plant in the culture box can receive the required light amount in the growth process, so that the healthy growth effect of the plant is guaranteed.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant cultivation, and in particular to a cultivation system and method for cultivating a corn plant resistant to bacterial wilt. BACKGROUND

[0002] Corn bacterial wilt is a plant disease caused by bacteria, and the main pathogen is Ralstonia solanacearum. This disease can infect the vascular system of corn plants, causing water and nutrient transport to be blocked, resulting in wilting, dwarfing and stem rot of corn plants. Once corn bacterial wilt occurs, it is difficult to control effectively, and the pathogen can survive in the soil for a long time. Therefore, cultivating and planting disease-resistant varieties is one of the most economical, effective and environmentally friendly strategies to prevent and control the disease.

[0003] In the process of cultivating corn plants resistant to bacterial wilt, an incubator is needed. The incubator usually has a lighting device inside. In the prior art, the lighting device in the incubator is either fixedly installed inside the incubator or there is relative movement between the lighting device and the component carrying the plants. The fixedly installed lighting device cannot uniformly illuminate all the plants, which can cause uneven growth of the plants. Although the relative movement between the lighting device and the component carrying the plants can ensure relatively uniform illumination between each plant, the lighting device is a homogeneous output. However, the amount of light required by each plant during growth is different. The homogeneous output of the lighting device can cause plants that require excessive light to not receive enough light, resulting in poor development, and plants that do not require too much light to die due to excessive light. SUMMARY

[0004] The purpose of the present application is to provide a cultivation system and method for cultivating corn plants resistant to bacterial wilt, which can ensure that each plant in the incubator receives the amount of light it needs during growth, thereby ensuring the healthy growth of the plants.

[0005] In a first aspect, the present application provides a cultivation system for cultivating corn plants resistant to bacterial wilt, which adopts the following technical solution:

[0006] The box body is internally provided with at least one partition plate, which divides the internal space of the box body into a plurality of independent spaces. Each independent space is slidably provided with a culture module, which can be pulled out and pushed into the independent space in a drawer manner to provide a culture environment that can be independently operated and physically isolated for plants.

[0007] A plurality of illumination mechanisms correspond to a plurality of independent spaces, which include an LED unit matrix board and a contour scanning assembly, the LED unit matrix board is arranged at the upper end of the internal space of the culture module, and the contour scanning assembly is located on the inner wall of the culture module, and the contour scanning assembly is used to obtain the overhead contour map of the plant canopy.

[0008] A plurality of observation mechanisms correspond to a plurality of independent spaces, which include a transparent culture plate for carrying plants and a camera assembly, the transparent culture plate and the camera assembly are located inside the culture module, the transparent culture plate is a hollow structure, and the roots of the plants are located inside the transparent culture plate, and the camera assembly is used to take pictures of the roots of the plants in the transparent culture plate.

[0009] Optionally, the contour scanning assembly includes two mounting frames, a sliding frame, a light curtain emitter and a photosensitive array receiver, the two mounting frames are fixed on the upper end inner wall of the culture module, and are arranged on the two sides of the LED unit matrix board, the sliding frame is slidably arranged on the mounting frame along the length direction of the mounting frame, the light curtain emitter is slidably arranged on the sliding frame along the length direction of the sliding frame, the mounting frame is provided with a first driving member for driving the sliding frame to slide, the sliding frame is provided with a second driving member for driving the light curtain emitter to slide, and the photosensitive array receiver is laid on the bottom of the culture module and used to receive the linear light emitted by the light curtain emitter.

[0010] Optionally, the transparent culture plate is a flat hollow structure, and the opposite two sides thereof are made of transparent material, a plurality of mounting holes with flexible silicone rubber sealing plugs are arranged on the upper end surface of the transparent culture plate, the mounting holes are in communication with the internal space of the transparent culture plate, and the root system of the plants is configured to grow in the hollow structure of the transparent culture plate, the bottom of the transparent culture plate is provided with an atomization device, the atomization device is used to generate nutrient gas mist inside the transparent culture plate, a plurality of transparent culture plates are provided, the plurality of transparent culture plates are uniformly and spaced apart in the horizontal direction, one end of each transparent culture plate is rotatably installed on the inner wall of the culture module, and the inner wall of the culture module is further provided with a third driving member for simultaneously driving all the transparent culture plates to rotate, and the camera assembly is fixedly installed on the outer wall of the light curtain emitter.

[0011] Optionally, at least one sealing operation hole with a self-healing injection septum is arranged on the transparent material of the transparent culture plate, and the operation hole is in communication with the internal space of the transparent culture plate.

[0012] Optionally, the upper end, the lower end and the end close to the back of the box of the culture module are open, when the culture module is inserted into the independent space, a closed space is formed between the culture module and the partition plate and the inner wall of the box, and the culture module is provided with a plug, the inner wall of the box is provided with a plurality of jack, the plurality of jack and the plug on the plurality of culture module one-to-one correspondence.

[0013] Optionally, the inner wall of the box is further provided with a plurality of groups of air holes, the plurality of groups of air holes correspond to the plurality of independent spaces one-to-one, and the plurality of groups of air holes are located in the independent space, the air hole includes air inlet and air outlet.

[0014] Optionally, the inner wall of the box is further provided with a plurality of liquid injection devices, the plurality of liquid injection devices correspond to the plurality of independent spaces one-to-one, and the liquid injection device is located in the independent space, the liquid injection device includes a plurality of liquid injection needles, the plurality of liquid injection needles correspond to the plurality of transparent culture plates in each independent space one-to-one, the rotation connecting axis between the liquid injection needle, the transparent culture plate and the culture module is coaxially arranged, and the end of the transparent culture plate opposite to the liquid injection needle is provided with a liquid injection hole of self-healing injection diaphragm, the liquid injection hole is in communication with the inner space of the transparent culture plate.

[0015] Optionally, the inner wall of the box is further provided with a plurality of groups of sensor assemblies, the plurality of groups of sensor assemblies correspond to the plurality of independent spaces one-to-one, and the sensor assembly is located in the independent space, the sensor assembly includes temperature sensor and humidity sensor.

[0016] In the second aspect, the application provides a method for cultivating a corn plant resistant to bacterial wilt, based on the cultivation system of the corn plant resistant to bacterial wilt in the first aspect, comprising the following steps:

[0017] S1: inserting the root of the plant to be cultured into the transparent culture plate through the installation hole, then sliding the culture module into the independent space, inserting the plug on the culture module into the jack on the box, and inserting the liquid injection needle on the inner wall of the box into the liquid injection hole on the transparent culture plate;

[0018] S2: starting the equipment, the control center controls the corresponding components to inject liquid into the transparent culture plate, starts the atomization device and the air circulation system, and adjusts the temperature and humidity of the air input into the independent space according to the data detected by the temperature sensor and the humidity sensor in the independent space;

[0019] S3: The control center will periodically obtain the top view contour data of the plant canopy from the contour scanning component. Based on the top view contour data, the control center will dynamically adjust the switching of each LED on the LED unit matrix board, turning on the LEDs directly above the plant canopy and turning off the LEDs above the gaps between plants. As the plants grow and the plant canopy increases, the control center will turn on the LEDs in the corresponding positions after receiving new top view contour data.

[0020] S31: The control center will periodically activate the third drive unit and rotate the transparent culture plate 180 degrees so that the plant roots will be close to the transparent plate on one side due to gravity. Then, the appearance data of the plant roots will be obtained through the camera component. Then, the experimenters can know the growth status of the plant through the appearance data of the plant canopy and the appearance data of the plant roots. At the same time, they can change the light intensity of the corresponding LED according to the plant growth status.

[0021] In summary, this application includes at least one of the following beneficial technical effects:

[0022] 1. By setting up the LED unit matrix board and contour scanning component, this invention can achieve precise lighting of plants according to the actual size and contour of the plant canopy, illuminating only the areas necessary for plant growth. This completely changes the traditional homogenized lighting mode of the cultivation box, avoiding ineffective irradiation of blank areas between plants, thereby significantly reducing power consumption. At the same time, the growth status of the plant can also be known according to the size of the plant canopy, so as to selectively turn on or off the LEDs above the plant so that the plant can obtain adequate light, ensure healthy plant growth, and improve the plant survival rate.

[0023] 2. The transparent culture plate of this invention is equipped with an atomizing device, which can directly supply nutrients to the root system in the form of aerosol, resulting in high absorption efficiency and no substrate residue, thus saving water and fertilizer. More importantly, the camera component of this invention can also directly observe whether the nutrients in the transparent culture plate are sufficient. For transparent culture plates with sufficient nutrients, there is no need to add nutrients, while for transparent culture plates lacking nutrients, nutrients are delivered in a timely manner, thereby achieving on-demand supply of nutrients and avoiding waste. In addition, compared with traditional technologies, with this invention, researchers can directly obtain first-hand data such as root morphology, growth rate, and health status without destructive seedling pulling or sampling, greatly improving the depth and accuracy of root research.

[0024] 3. The control center in this invention can also compare the plant's canopy data and root system data. If it detects poor root growth or stress in a plant while its canopy growth remains vigorous, it indicates that the plant is in an unhealthy "overdraft" growth state. In this case, the control center will autonomously and selectively reduce the light intensity above the plant's canopy, actively slowing down photosynthesis to alleviate root absorption pressure and induce the plant to allocate more energy to root growth, thereby helping the plant restore a healthy root-canopy balance.

[0025] 4. The drawer-type culture module in this invention allows for sampling, transplanting, or processing of any module without opening the main chamber, achieving "zero interference" with the environment (temperature, humidity, CO2 concentration) of other ongoing experimental groups. This ensures environmental stability during long-term culture experiments and improves the accuracy and reproducibility of experimental data. Simultaneously, the drawer-type culture module design allows for high-density, three-dimensional layouts, completely freeing up the large amount of redundant space reserved in traditional shelf-type designs for ease of manual operation, thus multiplying the effective culture throughput per unit area.

[0026] 5. The contour scanning component and the relatively enclosed cultivation module in this embodiment enable the contour scanning component to not only detect the plant canopy data, but also to calculate the "canopy crowding" within the entire module. When excessive crowding is detected and the leaves of neighboring plants begin to shade each other, the control center no longer simply lights up the top of each plant. Instead, the control center automatically enters a "shadow avoidance" mode: it slightly increases the light intensity and the proportion of a specific spectrum (such as far-red light) in the edge area (LED unit matrix board) of the cultivation module to simulate the "forest window effect," inducing the plants at the edge to grow outward. This proactively and non-contactly optimizes the canopy structure within the entire module, reduces excessive competition in the central area, and thus ensures healthy plant growth as much as possible. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the overall structure of Embodiment 1 of this application;

[0028] Figure 2 yes Figure 1 A schematic diagram of the overall structure with the cabinet door concealed in the center;

[0029] Figure 3 This is a schematic diagram of the internal structure of the box in Embodiment 1 of this application;

[0030] Figure 4 This is a schematic diagram of the sliding connection between the box and the culture module in Embodiment 1 of this application;

[0031] Figure 5This is a schematic diagram of the internal structure of the contour scanning component in Embodiment 1 of this application;

[0032] Figure 6 This is a schematic diagram of the module structure in Embodiment 1 of this application;

[0033] Figure 7 This is a schematic diagram of the structure of the third driving component in Embodiment 1 of this application;

[0034] Figure 8 This is a schematic diagram of the structure of the transparent culture plate in Embodiment 1 of this application;

[0035] In the diagram, 1. Box body; 11. Box door; 12. Divider plate; 13. Air inlet; 14. Air outlet; 15. Injection needle; 16. Solenoid valve; 17. Temperature sensor; 18. Humidity sensor; 2. Culture module; 21. Plug; 22. Slide groove; 23. Connecting groove; 3. Illumination mechanism; 31. LED unit matrix board; 32. Contour scanning component; 321. Mounting bracket; 322. Sliding bracket; 323. Light curtain emitter; 324. Photosensitive array receiver; 325. First driving component; 326. Second driving component; 4. Observation mechanism; 41. Transparent culture plate; 411. Mounting hole; 412. Sealing operation hole; 413. Injection hole; 42. Camera component; 43. Atomizing device; 44. Third driving component; 441. Gear; 442. Rack; 443. Electric push rod; 444. Connecting rod; 445. Rotating shaft. Detailed Implementation

[0036] The following is in conjunction with the appendix Figures 1-8 This application will be described in further detail below. Example 1

[0037] A breeding system for maize plants resistant to bacterial wilt, referring to... Figures 1-8 It includes a housing 1, multiple lighting mechanisms 3 and multiple observation mechanisms 4.

[0038] Reference Figure 1 , Figure 2 and Figure 3 In this embodiment, the front end of the box 1 is provided with a box door 11, and a control center operation screen is provided on the box 1 above the box door 11. The interior of the box 1 is provided with two partitions 12 along the vertical direction. The two partitions 12 divide the interior space of the box 1 into three independent spaces. A cultivation module 2 is slidably arranged in each independent space. In this embodiment, the cultivation module 2 can be pulled out and pushed in from the independent space in the manner of a drawer. The cultivation module 2 is used to carry the plant. When the cultivation module 2 is pushed into the independent space, the cultivation module 2, the inner wall of the box 1 and the partition 12 form a relatively closed space, which is convenient for providing the plant with an independently operable and physically isolated cultivation environment.

[0039] In this embodiment, three lighting mechanisms 3 are provided, each corresponding to one of the three independent spaces. Each lighting mechanism 3 includes an LED unit matrix board 31 and a contour scanning component 32. The LED unit matrix board 31 is fixedly installed on the top wall of the independent space, and the contour scanning component 32 is also installed on the top wall of the independent space. The contour scanning component 32 is used to obtain a top view contour image of the plant canopy. Both the LED unit matrix board 31 and the contour scanning component 32 are electrically connected to the control center. In this embodiment, the LED unit matrix board 31 consists of a large PCB and high-density soldered independently addressable RGBW LED units (such as the WS2812B type). Each LED unit can emit light independently and the light intensity can be adjusted independently.

[0040] In this embodiment, there are three observation mechanisms 4, each corresponding to one of the three independent spaces. Each observation mechanism 4 includes a transparent culture plate 41 for supporting plants and a camera component 42. The transparent culture plate 41 is installed on the inner wall of the culture module 2, and the camera component 42 is located inside the culture module 2. In this embodiment, the transparent culture plate 41 has a hollow structure. When the plant is transplanted onto the transparent culture plate 41, the roots of the plant are located inside the transparent culture plate 41. The camera component 42 is used to take pictures and record the plant roots inside the transparent culture plate 41.

[0041] When plants need to be cultivated, they are first transplanted onto the transparent cultivation plate 41, and then the drawer-type cultivation module 2 is pushed into the independent space for cultivation. During the cultivation process, the contour scanning component 32 at the top of the independent space periodically scans the canopy of each plant to obtain a top-view contour image of the plant canopy at different growth stages. Since the size and contour of the plant canopy are different at different stages, in the early stage of the plant, the photosynthesis of the plant is limited due to the small canopy. Therefore, the light intensity of the LED unit matrix plate 31 can be adjusted according to the size of the canopy to provide reasonable light intensity for the plant at this stage. At the same time, since the canopy of the plant is small, the gap between the plants is relatively large. Some of the light emitted by the LED unit matrix plate 31 does not shine on the canopy of the plant, but passes through the gap between the plants to the bottom of the independent space, resulting in a waste of light resources. In this embodiment, the control center of the device will analyze the position information of each plant, the size information of the plant canopy, and the gap between the plants based on the top-view contour image obtained by the contour scanning component 32. This device uses small amounts of information to independently control each individual LED unit in the LED unit matrix board 31. LED units located directly above the plant canopy are turned on, while those above the gaps between plants are turned off or have extremely low brightness. Therefore, during cultivation, as the plant canopy grows, the control center activates more LED units and increases their brightness, ensuring sufficient light for better plant growth. Simultaneously, the device precisely delivers the light energy released by the LED unit matrix board 31 to the plants, avoiding ineffective irradiation of blank areas and maximizing energy utilization. Precise lighting also reduces light irradiation on the moist transparent culture plate 41, effectively inhibiting algae growth. Furthermore, the periodically scanned series of plant canopy outlines are valuable phenotypic data that can be used to quantitatively analyze the rate of plant canopy expansion, providing crucial data for the experiment. The camera component 42 also periodically photographs the root system of each plant to obtain more and more important data for the experiment.

[0042] It should be further explained that the working cycle of the contour scanning component 32 and the camera component 42 can be set to be the same, so as to obtain the canopy and root information of the plant within the same period. This makes it easier for the control center or experimental personnel to obtain more accurate plant growth status. When the control center or experimental personnel find that the root growth vitality of a certain plant is lower than that of other plants (i.e., the root development is slow), the plant will be marked as "poor" by the system. When the control center retrieves this "poor" signal or manually inputs the "poor" signal, it will retrieve the scanning data of the canopy contour of the plant by the contour scanning component 32 in the past 24 hours and calculate the growth rate of canopy area (ΔArea / Δt). At this time, a simplified "Plant Health Index (PHI)" mathematical model is activated:

[0043] Where is the input root health score (e.g., "poor" = 0.3), A is the canopy area growth rate function, i.e., A = ΔArea / Δt, which is the canopy area growth rate of the plant under ideal conditions, and is the canopy area growth rate of the actual plant.

[0044] The biological significance of this model is that healthy plants should maintain a certain balance between root growth and canopy growth. If the root system is poor (low) while the canopy growth is rapid (low), the PHI will become very low or even negative, indicating that the plant is in an unhealthy "overdraft" state. When the control center calculates that the PHI value is far below the healthy threshold, it will immediately trigger an active intervention. Instead of meeting the canopy's light requirements at 100%, the control center will actively reduce the overall brightness of the LED units above the plant's canopy by 25% to slow down the plant's growth, promote energy transfer to the roots, and thus ensure the plant's survival as much as possible.

[0045] Reference Figure 3 , Figure 4 and Figure 5 In this embodiment, the contour scanning component 32 includes two mounting brackets 321, a sliding bracket 322, a light curtain emitter 323, and a photosensitive array receiver 324.

[0046] Two mounting brackets 321 are fixed to the upper inner wall of the cultivation module 2 and are arranged opposite each other on both sides of the LED unit matrix plate 31. A sliding bracket 322 is slidably disposed at the lower end of the mounting bracket 321 along the length direction of the mounting bracket 321. A light curtain emitter 323 is slidably disposed at the lower end of the sliding bracket 322 along the length direction of the sliding bracket 322. A first driving member 325 for driving the sliding bracket 322 to slide is provided on the mounting bracket 321. In this embodiment, the first driving member 325 is set as two electric push rods 443, and the two electric push rods 443 correspond one-to-one with the two mounting brackets 321. The electric push rods 443 are fixedly installed on the lower end face of the mounting bracket 321, and the first driving member 325 contains... The electric push rod 443 is located relatively far away from the door 11. The two electric push rods 443 in the first drive member 325 correspond one-to-one with the two ends of the sliding frame 322. The output end of the electric push rod 443 in the first drive member 325 is fixedly connected to the sliding frame 322. The sliding frame 322 is provided with a second drive member 326 for driving the light curtain emitter 323 to slide. In this embodiment, the second drive member 326 is also set as an electric push rod 443. The output end of the electric push rod 443 in the second drive member 326 is fixedly connected to the light curtain emitter 323. The photosensitive array receiver 324 is laid at the bottom of the cultivation module 2 for receiving the linear light emitted by the light curtain emitter 323.

[0047] It should be noted that the light curtain emitter 323 is a low-power line laser module that can project a thin and uniform "light blade" downwards. The photosensitive array receiver 324 is a large printed circuit board (PCB) laid on the bottom of each cultivation module 2. A large number of photosensitive diodes (such as TEMD5510FX01) are soldered on the PCB in a matrix, and the position coordinates of each diode are known. This is existing technology and will not be described in detail here. At the same time, in this embodiment, the first driving unit 325, the second driving unit 326, the light curtain emitter 323 and the photosensitive array receiver 324 are all electrically connected to the control center.

[0048] When a top-view outline of the plant canopy within a cultivation module 2 is needed, the control center activates the first drive unit 325 and the second drive unit 326, moving the light curtain emitter 323 to its initial coordinate position (the two-dimensional coordinate position of each diode on the photosensitive array receiver 324). Then, the light curtain emitter 323 continuously projects a "light blade" downwards. When the "light blade" illuminates a blank area without plants, the light penetrates and illuminates the photosensitive array receiver 324 at the bottom. The illuminated photosensitive diodes output a high level ("bright"). When the "light blade" is blocked by any part of the plant canopy, the photodiode below it cannot receive light and outputs a low-level ("dark") signal. After the light curtain emitter 323 completes the scanning work in one coordinate area, the second drive 326 moves, driving the light curtain emitter 323 to the next horizontally adjacent coordinate area. After completing one row of scanning, the first drive 325 drives the slide and light curtain emitter 323 to step one unit distance along the Y-axis (as shown in the figure), and then reverses along the X-axis to scan the next row. This process is repeated until the entire cultivation area is covered. After the scanning is completed, the control center integrates all the "bright / dark" signals collected by the photodiodes during the scanning process and generates a binary, high-resolution bitmap in memory, that is, obtains a relatively accurate, unobstructed top-view outline of the plant canopy.

[0049] Reference Figure 6 , Figure 7 and Figure 8In this embodiment, the transparent culture plate 41 has a flat, hollow structure, arranged along the Y-axis, and its opposite sides are made of transparent material (polycarbonate plate). Polycarbonate material has excellent impact resistance, high light transmittance, and good chemical stability, making it suitable for long-term use. The upper surface of the transparent culture plate 41 has multiple mounting holes 411 with flexible silicone rubber sealing plugs (not shown in the figure). The mounting holes 411 communicate with the internal space of the transparent culture plate 41, and the plant roots are configured to grow inside the hollow structure of the transparent culture plate 41. Each transparent culture plate 41 has a misting device 43 at its bottom. In this embodiment, each transparent culture plate 41 has five misting devices 43 arranged along its length. To integrate multiple piezoelectric ceramic ultrasonic atomizing plates for atomizing the introduced nutrient solution into micron-sized aerosol particles, five transparent culture plates 41 are provided in this embodiment. The five transparent culture plates 41 are evenly spaced along the Y-axis. The end of the transparent culture plate 41 near the door 11 is rotatably mounted on the inner wall of the culture module 2. The inner wall of the culture module 2 is also provided with a third driving component 44 that simultaneously drives all transparent culture plates 41 to rotate. The camera component 42 in this embodiment can be a common industrial camera or a webcam. The lens of the industrial camera or webcam is equipped with a high-pass optical filter. The camera component 42 is fixedly mounted on the outer wall of the light curtain transmitter 323. The third driving component 44 and the camera component 42 are both electrically connected to the control center.

[0050] When it is necessary to photograph and record the plant roots inside the transparent culture plate 41, the control center activates the third drive unit 44, which drives the five transparent culture plates 41 to rotate 90 degrees in the same direction. The roots that were originally suspended inside the transparent culture plate 41 will, under the action of gravity, gently, completely, and in their natural extended form, lie flat and attach to the other transparent plate (which is now located below). Then the camera component 42 can take pictures of the roots of each plant according to the movement trajectory of the light curtain transmitter 323 and transmit the pictures to the control center. The core principle of this structure is to solve the problem of root visualization by cleverly combining gravity and 90-degree horizontal axis rotation. During normal cultivation, the roots grow freely in the cavity; when observation is needed, the roots are rotated and gravity is used to make them naturally and gently adhere to one of the transparent plates, thereby achieving clear planar observation. It should be noted that the attached diagram in this embodiment is only for displaying the functional structure. In the actual device, there is sufficient distance between each transparent culture plate 41 so that when all transparent culture plates 41 are rotated to the horizontal, adjacent transparent culture plates 41 will not come into contact, and adjacent transparent culture plates 41 will not squeeze the plants.

[0051] The third driving component 44 in this embodiment includes a rotating shaft 445, a gear 441, a rack 442, and an electric push rod 443.

[0052] The culture module 2 has a horizontally oriented groove 22 on the side near the door 11. An electric push rod 443 and a rack 442 are installed inside the groove. The rack 442 is oriented in the same direction as the groove 22 and can slide along the groove 22. One end of the rack 442 is fixedly connected to the output end of the electric push rod 443. In this embodiment, five rotating shafts 445 are provided, each corresponding to one of the five transparent culture plates 41. One end of the rotating shaft 445 is fixedly connected to the transparent culture plate 41, and the other end is rotatably connected to the side wall of the culture module 2 near the box 1. The end of the rotating shaft 445 away from the transparent culture plate 41 extends into the groove 22. A gear 441 is coaxially fixedly connected to the end of the rotating shaft 445 located in the groove 22. All five gears 441 on the rotating shaft 445 mesh with the rack 442.

[0053] When it is necessary to drive all the transparent culture plates 41 to rotate, the electric push rod 443 is activated. The electric push rod 443 drives the rack 442 to slide in a direction away from itself. The rack 442 can then drive the five gears 441 to rotate at the same time, thereby driving the five transparent culture plates 41 to rotate.

[0054] In addition, the transparent culture plate 41 in this embodiment has at least one sealed operation hole 412 with a self-healing injection diaphragm (not shown) on its transparent material. The operation hole is connected to the internal space of the transparent culture plate 41. Experimenters can use a syringe to accurately inoculate or administer drugs to specific root segments through the transparent plate, so that experimenters can conduct more experiments.

[0055] Reference Figure 2 , Figure 3 and Figure 4 In this embodiment, the culture module 2 is U-shaped, with openings at its top, bottom, and the end near the back of the box 1. When the culture module 2 is slidably inserted into the independent space, a closed space is formed between the culture module 2, the partition plate 12, and the inner wall of the box 1. A plug 21 is provided at the end of the culture module 2 away from the door 11. Three sockets are provided on the inner wall of the box 1, and the three sockets correspond one-to-one with the plugs 21 on the three culture modules 2. When the culture module 2 is slidably inserted into the independent space, the plug 21 on the culture module 2 will automatically insert into the socket on the inner wall of the box 1, thereby enabling the culture module 2 to be connected to an external power source through the box 1. Therefore, the electrical components on the culture box 1 can be started.

[0056] It should be noted that the enclosure 1 itself is equipped with a refrigeration system, a heating system, a humidification system, and an air circulation system. The refrigeration system, heating system, and humidification system are all located within the air circulation system's duct (all existing technologies, which will not be elaborated upon here). In this embodiment, the inner wall of the enclosure 1 opposite the door 11 also has three sets of air vents. These three sets of air vents correspond one-to-one with three independent spaces, and each air vent is located within an independent space. The air vents include inlets 13 and outlets 14. All inlets 13 and outlets 14 are connected to the air circulation system's duct within the enclosure 1 itself. Furthermore, in this embodiment, the inlets 13 and outlets 14 of the three sets of air vents are connected in parallel. Therefore, the three independent spaces in this embodiment can share a single air circulation system. Additionally, each outlet 14 and each inlet... Each of the holes 13 is equipped with a solenoid valve 16, which is electrically connected to the control center. The solenoid valve 16 can control the opening and closing of each vent, thus preventing gas flow between the three independent spaces and allowing for independent cultivation experiments in each space. In addition, in this embodiment, three sets of sensor assemblies are also installed on the inner wall of the box 1. The three sets of sensor assemblies correspond one-to-one with the three independent spaces and are located within the independent spaces. The sensor assemblies include a temperature sensor 17 and a humidity sensor 18. The temperature sensor 17 and humidity sensor 18 in each set of sensor assemblies can detect the temperature and humidity in their respective independent spaces, so that the control center can promptly start the cooling system, heating system, or humidification system to provide a better growth environment for the plants in the cultivation module 2.

[0057] Finally, in this embodiment, three injection devices are also provided on the inner wall of the box 1 facing the box door 11. The three injection devices correspond one-to-one with the three independent spaces, and the injection devices are located in the independent spaces. In this embodiment, the injection devices include five injection needles 15, which correspond one-to-one with the five transparent culture plates 41 in each independent space. The injection needles 15 are coaxially arranged with the rotation shaft 445 on the transparent culture plate 41, and the transparent culture plate 41 has an injection hole 413 for a self-healing injection diaphragm at one end facing the injection needle 15. The injection hole 413 is connected to the internal space of the transparent culture plate 41. When the culture module 2 is slidably pushed into the independent space, the independent space... The injection needles 15 on the inner wall of the space will be inserted into the injection holes 413 of the corresponding transparent culture plate 41. Each injection needle 15 is connected to the external liquid supply device, which can supply liquid to each injection needle 15 individually. Therefore, the remaining amount of nutrient solution in each transparent culture plate 41 and the growth status of the plant roots can be known through the photo information taken by the camera component 42. The control center can control the liquid supply device to accurately supply liquid to each transparent culture plate 41, thereby avoiding the situation where the plant roots rot due to excessive nutrient solution in the transparent culture plate 41, or the plant cannot grow due to insufficient nutrient solution in the transparent culture plate 41. Example 2

[0058] A method for cultivating maize plants resistant to bacterial wilt, based on a cultivation system for maize plants resistant to bacterial wilt in Example 1, is characterized by comprising the following steps:

[0059] S1: Insert the roots of the plant to be cultivated into the transparent culture plate 41 through the mounting hole 411, then slide the culture module 2 into the independent space, insert the plug 21 on the culture module 2 into the socket on the box 1, and insert the injection needle 15 on the inner wall of the box 1 into the injection hole 413 on the transparent culture plate 41.

[0060] S2: Start the equipment. The control center controls the corresponding components to inject liquid into the transparent culture plate 41. At the same time, the atomizing device 43 and the air circulation system are started. Based on the data detected by the temperature sensor 17 and humidity sensor 18 in the independent space, the temperature and humidity of the air input into the independent space are adjusted.

[0061] S3: The control center will periodically obtain the top view contour data of the plant canopy from the contour scanning component 32. Based on the top view contour data, the control center will dynamically adjust the switching or power of each LED on the LED unit matrix board 31, turn on the LED directly above the plant canopy, and turn off or dim the LEDs above the gaps between plants. As the plant grows and the plant canopy increases, the control center will turn on or brighten the LEDs in the corresponding positions after receiving new top view contour data.

[0062] S31: The control center will periodically activate the third drive unit 44 and drive the transparent culture plate 41 to rotate 180 degrees, so that the plant roots will be close to the transparent plate on one side due to gravity. Then, the appearance data of the plant roots will be obtained through the camera component 42. Then, the experimenters can know the growth status of the plant through the appearance data of the plant canopy and the appearance data of the plant roots. At the same time, they can change the light intensity of the corresponding LED according to the plant growth status.

[0063] The embodiments described in this specific implementation are preferred embodiments of this application and are not intended to limit the scope of protection of this application. Identical components are represented by the same reference numerals. Therefore, all equivalent changes made to the structure, shape, and principle of this application should be covered within the scope of protection of this application.

Claims

1. A cultivation system for maize plants resistant to bacterial wilt, characterized in that, include: The box (1) has at least one partition (12) inside, which divides the internal space of the box (1) into multiple independent spaces. A cultivation module (2) is slidably arranged in each independent space. The cultivation module (2) can be pulled out and pushed in from the independent space in a drawer manner to provide the plant with an independently operable and physically isolated cultivation environment. Multiple lighting mechanisms (3) correspond one-to-one with multiple independent spaces. Each includes an LED unit matrix plate (31) and a contour scanning component (32). The LED unit matrix plate (31) is set on the upper surface of the internal space of the cultivation module (2). The contour scanning component (32) is located on the inner wall of the cultivation module (2) and is used to obtain a top view contour of the plant canopy. Multiple observation units (4) correspond one-to-one with multiple independent spaces, including a transparent culture plate (41) for supporting plants and a camera component (42). The transparent culture plate (41) and the camera component (42) are both located inside the culture module (2). The transparent culture plate (41) is a hollow structure, and the roots of the plants are located inside the transparent culture plate (41). The camera component (42) is used to take pictures and record the roots of the plants inside the transparent culture plate (41). The contour scanning component (32) includes two mounting brackets (321), a sliding bracket (322), a light curtain emitter (323), and a photosensitive array receiver (324). The two mounting brackets (321) are fixed on the upper inner wall inside the cultivation module (2) and are arranged opposite to each other on both sides of the LED unit matrix plate (31). The sliding bracket (322) is slidably mounted on the mounting bracket (321) along the length direction of the mounting bracket (321). The light curtain emitter (323) is slidably mounted on the sliding bracket (322) along the length direction of the sliding bracket (322). The mounting bracket (321) is provided with a first driving member (325) for driving the sliding bracket (322) to slide. The sliding bracket (322) is provided with a second driving member (326) for driving the light curtain emitter (323) to slide. The photosensitive array receiver (324) is laid at the bottom inside the cultivation module (2) for receiving the linear light emitted by the light curtain emitter (323). The transparent culture plate (41) has a flat, hollow structure, and its opposite sides are made of transparent material. The upper surface of the transparent culture plate (41) has multiple mounting holes (411) with flexible silicone rubber sealing plugs. The mounting holes (411) communicate with the internal space of the transparent culture plate (41), and the plant's roots are configured to grow inside the hollow structure of the transparent culture plate (41). The bottom of each transparent culture plate (41) is equipped with an atomizing device (43), which is used to... Nutrient mist is generated inside the transparent culture plate (41). Multiple transparent culture plates (41) are provided and are evenly spaced along the horizontal direction. One end of each transparent culture plate (41) is rotatably mounted on the inner wall of the culture module (2). The inner wall of the culture module (2) is also provided with a third driving component (44) that simultaneously drives all transparent culture plates (41) to rotate. The camera component (42) is fixedly mounted on the outer wall of the light curtain emitter (323).

2. The cultivation system for maize plants resistant to bacterial wilt according to claim 1, characterized in that, The transparent culture plate (41) has at least one sealed operation hole (412) with a self-healing injection diaphragm on its transparent material, and the operation hole is connected to the internal space of the transparent culture plate (41).

3. The cultivation system for maize plants resistant to bacterial wilt according to claim 1, characterized in that, The upper end, lower end and one end near the back of the box (1) of the culture module (2) are all open. When the culture module (2) is slidably inserted into the independent space, a closed space is formed between the culture module (2), the partition plate (12) and the inner wall of the box (1). The culture module (2) is provided with a plug (21) and the inner wall of the box (1) is provided with multiple sockets. The multiple sockets correspond one-to-one with the plugs (21) on the multiple culture modules (2).

4. The cultivation system for maize plants resistant to bacterial wilt according to claim 3, characterized in that, The inner wall of the box (1) is also provided with multiple sets of air holes, each set of air holes corresponding to multiple independent spaces, and each set of air holes is located in an independent space. The air holes include an air inlet (13) and an air outlet (14).

5. The cultivation system for maize plants resistant to bacterial wilt according to claim 3, characterized in that, The inner wall of the box (1) is also provided with multiple injection components, each corresponding to a single independent space. The injection components are located within the independent spaces. Each injection component includes multiple injection needles (15), each corresponding to a single transparent culture plate (41) in each independent space. The rotational connection axis between the injection needles (15), the transparent culture plate (41), and the culture module (2) is coaxially arranged. The transparent culture plate (41) has an injection hole (413) with a self-healing injection diaphragm at one end facing the injection needles (15). The injection hole (413) is connected to the internal space of the transparent culture plate (41).

6. The cultivation system for maize plants resistant to bacterial wilt according to claim 3, characterized in that, Multiple sets of sensor components are also provided on the inner wall of the box (1). Each set of sensor components corresponds to a single independent space, and the sensor components are located in the independent spaces. The sensor components include a temperature sensor (17) and a humidity sensor (18).

7. A method for cultivating maize plants resistant to bacterial wilt, based on the cultivation system for maize plants resistant to bacterial wilt as described in any one of claims 1-6, characterized in that, Includes the following steps: S1: Insert the roots of the plant to be cultured into the transparent culture plate (41) through the mounting hole (411), and then slide the culture module (2) into the independent space. Insert the plug (21) on the culture module (2) into the socket on the box (1), and insert the injection needle (15) on the inner wall of the box (1) into the injection hole (413) on the transparent culture plate (41). S2: Start the equipment. The control center controls the corresponding components to inject liquid into the transparent culture plate (41). At the same time, the atomizing device (43) and the air circulation system are started. The temperature and humidity of the air input into the independent space are adjusted according to the data detected by the temperature sensor (17) and humidity sensor (18) in the independent space. S3: The control center will periodically obtain the top view contour data of the plant canopy from the contour scanning component (32), and based on the top view contour data, the control center will dynamically adjust the switching of each LED on the LED unit matrix board (31), turn on the LED directly above the plant canopy, and turn off the LED above the gap between plants. As the plant grows and the plant canopy increases, the control center will turn on the LED in the corresponding position after receiving new top view contour data. S31: The control center will periodically activate the third drive unit (44) and drive the transparent culture plate (41) to rotate 180 degrees so that the plant roots will be close to the transparent plate on one side due to gravity. Then, the appearance data of the plant roots will be obtained through the camera component (42). Then, the experimenters can know the growth status of the plant through the appearance data of the plant canopy and the appearance data of the plant roots. At the same time, they can change the light intensity of the corresponding LED according to the plant growth status.

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