Cultivation method of aquilaria agallocha native small seed tree

Abstract

The invention relates to the technical field of plant cultivation, in particular to a cultivation method of an agilawood native small seed tree of aquilaria agilawood, which ensures that a male parent and a female parent are purebred aquilaria agilawood through artificial pollination, avoids hybridization with other agilawood tree species (such as aquilaria sinensis), adopts a specific seedling raising technology, detects genotypes through a molecular marker technology (such as SSR or SNP), and can be used for breeding the small seed tree of the aquilaria agilawood. The method comprises the following steps: removing seedlings (namely aquilaria sinensis seedlings) which do not conform to the genotype of the female parent of the Machilus davidii, ensuring that the genetic character of the seed seedlings of the Machilus davidii is stable, keeping a pure female parent gene, and directly cultivating the Aquilaria davidii tree instead of the aquilaria sinensis seedlings (aquilaria sinensis seedlings) without secondary grafting.

Description

Technical Field

[0001] The present invention relates to the technical field of plant cultivation, and in particular to a method for cultivating native young mother trees of Aquilaria sinensis. Background Art

[0002] Agarwood is the top variety of agarwood, with extremely high economic and medicinal value. In traditional agarwood cultivation, directly using natural agarwood seeds to cultivate seedlings will cause mutations, and the resulting agarwood seedlings are all native agarwood seedlings rather than agarwood seedlings. In order to obtain agarwood seedlings, it is often necessary to obtain them through grafting, that is, using white agarwood (native agarwood tree) as the rootstock and grafting agarwood branches. This method has the following problems:

[0003] 1. Genetic instability: naturally pollinated agarwood seeds are prone to mutation, and their offspring degenerate into ordinary white agarwood, unable to maintain the characteristics of agarwood;

[0004] 2. Dependence on grafting: Existing technology requires two graftings to obtain agarwood seedlings, which increases the cost and time of cultivation;

[0005] 3. The quality of agarwood is uncontrollable: the rootstock and root system of the grafted seedlings are still white agarwood, which may affect the quality and yield of agarwood, and the rootstock to the root system cannot produce agarwood.

[0006] Therefore, there is an urgent need for a method to directly use agarwood seeds to cultivate purebred agarwood mother trees, ensure genetic stability, eliminate the need for grafting, and maintain the excellent traits of the mother plant. Summary of the Invention

[0007] In view of the above situation, in order to overcome the defects of the existing technology, the present invention provides a method for cultivating native small mother trees of Aquilaria sinensis. Through artificial pollination, seed selection and specific cultivation techniques, the genetic stability of Aquilaria sinensis seedlings is ensured, and they are directly cultivated into purebred Aquilaria sinensis trees rather than white aquilaria sinensis seedlings, without the need for secondary grafting.

[0008] A method for cultivating a small native mother tree of Aquilaria sinensis, comprising the following steps:

[0009] 1 Artificial pollination seed preservation

[0010] 1.1 Select purebred agarwood mother trees with excellent traits as pollination parents, ensuring that both the male and female parents are purebred agarwood, and avoid hybridization with other agarwood species;

[0011] 1.2 Collect pollen from the male parent during the flowering period and pollinate the female parent flowers through artificial pollination;

[0012] 1.3 After the fruits are ripe, select healthy and plump agarwood seeds;

[0013] 2. Seed treatment and seedling cultivation

[0014] 2.1 Treat the seeds to promote germination;

[0015] 2.2 Sow in a loose and breathable seedling medium, maintain a humidity of 60%-70%, a temperature of 25-30℃, and avoid direct sunlight;

[0016] 2.3 After the seedlings have grown 3-5 true leaves, use molecular marker technology to detect genotypes and remove seedlings that do not conform to the genotype of the agarwood mother plant;

[0017] 3. Screening and cultivation of purebred agarwood seedlings

[0018] 3.1 Ensure that all cultivated seedlings are purebred agarwood through leaf morphology, smell and genetic testing;

[0019] 3.2 Transplanting, fertilization management, and promoting root development;

[0020] Furthermore, the germination promotion process is: soaking the seeds in warm water at 25-30°C for 24 hours to promote germination.

[0021] Furthermore, the seedling medium is made of a mixture of peat soil and vermiculite, and the weight ratio between the two is:

[0022] Peat soil: vermiculite = 3:1.

[0023] Furthermore, the transplanting and fertilization management process is as follows: transplant to nutrient pots or fields, use slightly acidic soil (pH 5.5-6.5), and regularly apply organic fertilizers and phosphorus and potassium fertilizers to promote root development.

[0024] The beneficial effects achieved by the present invention using the above structure are as follows:

[0025] 1. Genetic purity and stability: Through artificial pollination and genetic testing, we ensure that the agarwood seedlings are 100% pure agarwood, not white agarwood seedlings, to avoid natural variation.

[0026] 2. No need for grafting: Directly cultivate native agarwood seedlings, the roots and branches and leaves are all agarwood, no need for secondary grafting, reducing cost and time.

[0027] 3. High quality of agarwood: The agarwood production characteristics of purebred agarwood seedlings are better than those of grafted seedlings. They have a stronger aroma, richer oil content, higher yield, and can also produce agarwood at the roots.

[0028] 4. Suitable for large-scale planting: It provides standardized seedling raising process, which is suitable for large-scale planting. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0030] A method for cultivating native small mother trees of agarwood ensures the stability of genetic traits of agarwood seedlings and maintains pure maternal genes through artificial pollination and specific seedling cultivation technology. Agarwood seedlings can be directly cultivated into agarwood trees without secondary grafting, rather than white agarwood seedlings (native agarwood seedlings).

[0031] Example

[0032] A method for cultivating a small native mother tree of Aquilaria sinensis, comprising the following steps:

[0033] 1 Artificial pollination seed preservation

[0034] 1.1 Select purebred agarwood mother trees with excellent traits as pollination parents, ensure that both the male and female parents are purebred agarwood, and avoid hybridization with other agarwood species (such as white agarwood);

[0035] 1.2 Collect pollen from the male parent during the flowering period and pollinate the female parent flowers through artificial pollination to ensure genetic homozygosity and take measures to prevent mutations caused by natural hybridization;

[0036] 1.3 After the fruits are ripe, select healthy and plump agarwood seeds and remove underdeveloped or suspected mutant seeds;

[0037] 2. Seed treatment and seedling cultivation

[0038] 2.1 Soak the seeds in warm water at 25-30℃ for 24 hours to promote germination;

[0039] 2.2 Sow in a loose and breathable seedling medium (peat soil weight: vermiculite weight = 3:1), maintain a humidity of 60%-70%, a temperature of 25-30°C, and avoid direct sunlight;

[0040] 2.3 After the seedlings have grown 3-5 true leaves, use molecular marker technology (such as SSR or SNP) to detect genotypes and eliminate seedlings that do not match the genotype of the agarwood mother plant (i.e., white agarwood seedlings);

[0041] 3. Screening and cultivation of purebred agarwood seedlings

[0042] 3.1 Through leaf morphology, smell (the leaves of agarwood seedlings have a unique fragrance after rubbing) and genetic testing, we ensure that all cultivated seedlings are pure agarwood, not white agarwood seedlings;

[0043] 3.2 Transplant to nutrient pots or fields, use slightly acidic soil (pH 5.5-6.5), and regularly apply organic fertilizers and phosphorus and potassium fertilizers to promote root development;

[0044] 4 Grafting-free tree growing technology

[0045] 4.1 The agarwood seedlings cultivated by the present invention do not require grafting, and their roots, branches and leaves are all pure agarwood, which can fully retain the fragrance-bearing characteristics of the mother plant; through water control, light control and nutrition regulation, the lignification of the seedlings is accelerated and the tree-forming cycle is shortened;

[0046] 5. Fragrant induction

[0047] 5.1 After the saplings have grown for 1-2 years, physical trauma such as pruning and cutting can be used to induce agarwood to form on the wounds, and the agarwood blocks can be identified by visual, tactile, olfactory and taste senses.

[0048] It should be noted that, in this article, the terms "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or apparatus that includes a series of elements includes not only those elements, but also includes other elements not explicitly listed, or also includes elements that are inherent to such process, method, article or apparatus.

[0049] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A method for cultivating a small primary mother tree of Aquilaria sinensis, characterized by comprising the following steps: 1 Artificial pollination seed preservation 1.1 Select purebred agarwood mother trees with excellent traits as pollination parents, ensuring that both the male and female parents are purebred agarwood, and avoid hybridization with other agarwood species; 1.2 Collect pollen from the male parent during the flowering period and pollinate the female parent flowers through artificial pollination to ensure genetic homozygosity and take measures to prevent mutations caused by natural hybridization; 1.3 After the fruits are ripe, select healthy and plump agarwood seeds and remove underdeveloped or suspected mutant seeds; 2. Seed treatment and seedling cultivation 2.1 Soak the seeds in warm water to promote germination; 2.2 Sow in a loose and breathable seedling medium, maintain appropriate humidity and temperature, and avoid direct sunlight; 2.3 After the seedlings have grown 3-5 true leaves, use molecular marker technology to detect genotypes and remove seedlings that do not conform to the genotype of the agarwood mother plant; 3. Screening and cultivation of purebred agarwood seedlings 3.1 Through leaf morphology, smell and genetic testing, we ensure that all cultivated seedlings are purebred agarwood, not white agarwood seedlings; 3.2 Transplant to nutrient pots or fields, use slightly acidic soil, and regularly apply organic fertilizers and phosphorus and potassium fertilizers to promote root development; 4 Grafting-free tree growing technology 4.1 Accelerate the lignification of seedlings and shorten the tree-growing cycle by controlling water, light and nutrition; 5. Fragrant induction 5.1 After the saplings have grown for 1-2 years, physical trauma such as pruning and cutting can be used to induce agarwood to form on the wounds, and the agarwood blocks can be identified by visual, tactile, olfactory and taste senses.

2. The method for cultivating a primary mother tree of Aquilaria sinensis according to claim 1, characterized in that in step 2, the seedling medium is made of a mixture of peat soil and vermiculite; And the weight of the peat soil: the weight of the vermiculite = 3:

1.

3. The method for cultivating a primary mother tree of Aquilaria sinensis according to claim 1, characterized in that: In step 3, the pH of the slightly acidic soil is 5.5-6.

5.

4. The method for cultivating a primary mother tree of Aquilaria sinensis according to claim 1, characterized in that: In step 2, the temperature of the warm water is 25-30°C; The soaking time is 24 hours.

5. The method for cultivating a primary mother tree of Aquilaria sinensis according to claim 1, characterized in that: In step 2, the humidity of the seedling medium is 60%-70% and the temperature is 25-30°C.