A method for preparing a gel formulation for improving cervical HPV infection and establishing a preventive barrier

Abstract

The application discloses a gel preparation preparation method for improving cervical HPV infection and establishing a preventive barrier, relates to the technical field of gel preparation, and aims to solve the technical problems that the existing treatment methods are mostly focused on treating infected patients, and there are obvious deficiencies in the prevention of HPV infection, especially in establishing an effective preventive barrier in uninfected people, including: a crushing mechanism, which is mainly used for crushing raw materials, the crushing mechanism comprises a cabinet body, a cabinet door is arranged on one side of the cabinet body, a crushing barrel is arranged in the cabinet door, and a rotating motor matched with the crushing barrel is arranged on the cabinet door; a reverse osmosis mechanism, which is mainly used for controlling the osmotic pressure of the gel. The application has the advantages of activating local immune cells of the cervix, promoting cytokine secretion, enhancing the immune clearance ability of the body to HPV virus, providing long-acting preventive protection for the cervix, and reducing the risk of HPV infection.

Description

Technical Field

[0001] This invention relates to the field of gel preparation technology, and more specifically, to a method for preparing a gel preparation that improves cervical HPV infection and establishes a preventive barrier. Background Technology

[0002] Human papillomavirus (HPV) infection is a major challenge in global public health, especially among women's reproductive system diseases. According to data from the World Health Organization (WHO), approximately 99.7% of cervical cancer cases are associated with persistent infection with high-risk HPV types. Globally, there are over 600,000 new cases of cervical cancer and over 340,000 deaths annually, seriously threatening women's health and lives. HPV is epithelial-tropic and primarily transmitted through sexual contact. After infection, it can remain latent in the cervical mucosal epithelial cells for a long time, and some types (such as HPV16 and 18) are prone to inducing malignant transformation of cells. However, currently, there are no specific drugs for treating HPV infection, and existing treatment methods have significant limitations.

[0003] Currently, treatments for HPV infection mainly include surgical removal of lesions, physical therapy (such as laser, cryotherapy, and electrocautery), and drug therapy. While surgery and physical therapy can remove visible lesions, they cannot completely eliminate the virus, resulting in a high recurrence rate and potential damage to normal tissues, affecting organ function and appearance. Traditional drug therapies, such as immunomodulators and antiviral drugs, have limited efficacy and are difficult to achieve highly effective suppression and clearance of the HPV virus. Furthermore, most existing treatments focus on treating already infected patients, and are significantly insufficient in preventing HPV infection, especially in establishing an effective preventive barrier in uninfected individuals.

[0004] In view of this, we propose a method for preparing a gel formulation that improves cervical HPV infection and establishes a preventive barrier. Summary of the Invention

[0005] The purpose of this invention is to provide a method for preparing a gel formulation that improves cervical HPV infection and establishes a preventive barrier, so as to solve the technical problems mentioned in the background above.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a method for preparing a gel preparation for improving cervical HPV infection and establishing a preventive barrier, comprising:

[0007] The crushing mechanism is mainly used for crushing raw materials. The crushing mechanism includes a cabinet, a cabinet door on one side of the cabinet, a crushing barrel inside the cabinet door, and a rotary motor that works with the crushing barrel on the cabinet door.

[0008] The reverse osmosis mechanism is mainly used to control the osmotic pressure of the gel. The reverse osmosis mechanism is located on one side of the pulverizing mechanism and is connected to the pulverizing mechanism. The reverse osmosis mechanism includes a high-pressure pump, a pressure vessel is provided on the high-pressure pump, a long pipe is provided on the pressure vessel and is connected to the pulverizing barrel, and several filters are provided at the bottom of the pressure vessel. Each of the filters is connected to the high-pressure pump.

[0009] The stirring mechanism is mainly used for mixing and emulsifying the gel matrix. The stirring mechanism is located on the side of the reverse osmosis mechanism away from the crushing mechanism and is connected to the reverse osmosis mechanism. The stirring mechanism includes a stirring tank. A vacuum pump is provided on the side of the stirring tank away from the reverse osmosis mechanism. An air pipe is provided on the top of the vacuum pump. A radiator is connected to the air pipe, and a filter canister is provided at the bottom of the radiator.

[0010] The gel preparation is used to improve cervical HPV infection and establish a preventive barrier. The gel preparation is prepared by the following steps: S1, raw material pretreatment: Carbomer 940 is slowly and evenly sprinkled into an appropriate amount of physiological saline and stirred at room temperature to allow it to fully swell and form a uniform colloid. During the stirring process, care should be taken to avoid clumping.

[0011] S2. Mixing and reaction: Transfer the cooled hyaluronic acid solution to a vacuum emulsifying mixer, turn on the stirring function, and stir at an appropriate speed.

[0012] S3. Sterilization treatment: The gel, which is mixed evenly and has been adjusted in pH and viscosity, is filtered through a 0.22μm microporous membrane filter to remove microorganisms and impurities and ensure the sterility of the gel.

[0013] S4. Finished product quality inspection: On a sterile operating table, take an appropriate amount of the finished gel for microbial limit testing. Use equipment such as incubators and microscopes to determine indicators such as total bacterial count and mold count to ensure that it meets the microbial standards stipulated in the pharmacopoeia.

[0014] Preferably, the cabinet door is provided with a transparent observation window and a handle, the cabinet body is provided with a rotary handle for driving the pulverizing barrel to rise and fall, and the cabinet body has an installation groove on the side facing the reverse osmosis mechanism.

[0015] Preferably, the high-pressure pump is provided with a mounting bracket at the bottom, and three filters are provided, all of which are fixed to the bottom of the mounting bracket.

[0016] Preferably, the filters are interconnected and connected to a high-pressure pump via hoses, which in turn are connected to a pressure vessel via hoses.

[0017] Preferably, the air pipe is vertically arranged and placed on the side of the vacuum pump away from the mixing tank. The air pipe is equipped with two valves connected to the radiator, and the radiator is equipped with several screws.

[0018] Preferably, S1 includes:

[0019] S1.1 Slowly and evenly sprinkle Carbomer 940 into an appropriate amount of physiological saline, stir at room temperature to allow it to fully swell and form a uniform colloid, and take care to avoid clumping during the stirring process;

[0020] S1.2 Add chitosan to a 1% acetic acid solution, stir and dissolve at an appropriate temperature, then remove insoluble matter through a filter to obtain a clear chitosan solution for later use.

[0021] S1.3 Add hyaluronic acid to warm physiological saline, keeping the temperature between 40-50℃. At the same time, add glycerin and polysorbate-80. Use a heating stirrer to stir until all components are fully dissolved and a homogeneous solution is formed. Let the solution cool to room temperature before use.

[0022] Preferably, S2 includes:

[0023] S2.1 Transfer the cooled hyaluronic acid solution to a vacuum emulsifying mixer, turn on the stirring function, and stir at an appropriate speed;

[0024] S2.2 Add chitosan solution and carbomer colloid in sequence, and stir continuously for 30 minutes to ensure that all components are fully mixed.

[0025] S2.3 Dissolve interferon α-2b in a small amount of physiological saline beforehand. After the above mixture is stirred evenly, slowly add it to the mixture and continue stirring for 15 minutes. Avoid vigorous stirring during the process to prevent the inactivation of interferon α-2b protein.

[0026] S2.4 Use a pH adjustment device to test the pH value of the mixture. When the pH value deviates from the range of 5.5-6.5, use an automatic liquid addition device to accurately add acid or alkali solution to adjust the pH value until it reaches the specified range.

[0027] S2.5. According to the gel viscosity requirements, adjust the gel viscosity to 10000-15000 cP by adding a small amount of physiological saline or carbomer 940 at 25℃. Stir continuously during the adjustment process to ensure uniform viscosity.

[0028] Preferably, S3 includes:

[0029] S3.1. The gel, which has been mixed evenly and whose pH value and viscosity have been adjusted, is filtered through a 0.22μm microporous membrane filter to remove microorganisms and impurities, ensuring the sterility of the gel. During the filtration process, pay attention to the pressure changes of the filter to prevent clogging.

[0030] S3.2 Under sterile conditions, the filtered gel is precisely filled into medical gel tubes using an aseptic filling machine. Each tube is filled with 5g. The accuracy of the filling volume is controlled by a high-precision metering pump, and the gel tubes are sealed using an automatic sealing function to prevent contamination.

[0031] Preferably, S4 includes:

[0032] S4.1 Visually inspect the finished gel; it should be a colorless, transparent, impurity-free, uniformly textured gel-like substance, without layering or discoloration.

[0033] S4.2. Use a pH meter to accurately measure the pH value of the finished gel and ensure that it is within the range of 5.5-6.5; use a rotational viscometer to measure the gel viscosity at 25°C to determine whether it meets the standard requirement of 10000-15000 cP.

[0034] S4.3. Using an osmometer, the osmotic pressure of the gel is measured by the freezing point depression method or vapor pressure method to ensure that it is maintained at 280-310 mOsm / L.

[0035] S4.4 On a sterile operating table, take an appropriate amount of the finished gel for microbial limit testing. Use equipment such as an incubator and microscope to determine indicators such as total bacterial count and mold count to ensure that it meets the microbial standards stipulated in the pharmacopoeia.

[0036] Compared with the prior art, the beneficial effects of the present invention are:

[0037] 1. This invention utilizes a gel formulation with scientifically formulated components and precise manufacturing processes to effectively intervene in cervical HPV infection. Interferon α-2b, as the core antiviral component, maintains high activity in the formulation after being added through a specific process; its activity retention rate remains ≥80% even after 3 months of storage at 4°C. It can induce cells to produce antiviral proteins, inhibit HPV replication and transcription, and block viral proliferation within cervical cells. Simultaneously, chitosan has immunomodulatory effects, activating local cervical immune cells, promoting cytokine secretion, and enhancing the body's immune clearance capacity against HPV. The synergistic effect of these two components significantly improves the therapeutic effect against HPV infection compared to traditional single-component drugs. Clinical studies show that patients treated with this gel formulation experience a faster decrease in viral load and a more than 30% increase in the negative conversion rate.

[0038] 2. In the preparation process of this invention, hyaluronic acid and chitosan are mixed in a specific ratio (1:1.5) to form a gel matrix with excellent film-forming and mucosal adhesion properties. This matrix can rapidly form a dense physical barrier on the cervical mucosa. Experimental tests show that the film formation time is only 5-8 minutes, and the film layer can adhere to the cervix for 6-8 hours, effectively blocking the binding of HPV virus to receptors such as heparan sulfate on the surface of host cells, reducing the chance of viral invasion. In addition, polysorbate-80 in the gel enhances the dispersibility of each component, allowing the active substances to be evenly distributed in the film layer, continuously exerting antiviral and immunomodulatory effects, providing long-term preventive protection for the cervix, and reducing the risk of HPV infection. Attached Figure Description

[0039] Figure 1 This is a schematic diagram of the structure of the present invention;

[0040] Figure 2 For the present invention Figure 1 Schematic diagram of the crushing mechanism;

[0041] Figure 3 For the present invention Figure 1 A schematic diagram of the structure of a reverse osmosis unit;

[0042] Figure 4 For the present invention Figure 1 A schematic diagram of the stirring mechanism.

[0043] Explanation of the labels in the diagram: 1

[0044] 1. Crushing mechanism; 11. Cabinet; 12. Cabinet door; 13. Crushing drum; 14. Rotary motor; 2. Reverse osmosis mechanism; 21. High-pressure pump; 22. Pressure vessel; 23. Filter; 24. Long pipe; 3. Stirring mechanism; 31. Stirring drum; 32. Vacuum pump; 33. Gas pipe; 34. Radiator; 35. Filter canister. Detailed Implementation

[0045] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0046] In the description of the embodiments of the present invention, it should be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships, are based on the orientation or positional relationships shown in the accompanying drawings and are only for the convenience of describing the embodiments of the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined with "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the embodiments of the present invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0047] In the embodiments of the present invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of the present invention according to the specific circumstances.

[0048] like Figures 1 to 4 As shown, the present invention relates to a method for preparing a gel formulation for improving cervical HPV infection and establishing a protective barrier, comprising:

[0049] The crushing mechanism 1 is mainly used for crushing raw materials. The crushing mechanism 1 includes a cabinet 11, a cabinet door 12 is provided on one side of the cabinet 11, a crushing barrel 13 is provided inside the cabinet door 12, and a rotary motor 14 that works with the crushing barrel 13 is provided on the cabinet door 12. A transparent observation window is provided on the cabinet door 12, and a handle is provided on the cabinet door 12. A rotary handle that drives the crushing barrel 13 to rise and fall is provided on the cabinet 11, and an installation groove is provided on the side of the cabinet 11 facing the reverse osmosis mechanism 2.

[0050] The reverse osmosis mechanism 2 is mainly used to control the gel osmotic pressure. The reverse osmosis mechanism 2 is located on one side of the crushing mechanism 1 and is connected to the crushing mechanism 1. The reverse osmosis mechanism 2 includes a high-pressure pump 21. A pressure vessel 22 is provided on the high-pressure pump 21. A long pipe 24 connected to the crushing barrel 13 is provided on the pressure vessel 22. Several filters 23 are provided at the bottom of the pressure vessel 22. Each filter 23 is connected to the high-pressure pump 21. A mounting frame is provided at the bottom of the high-pressure pump 21. Three filters 23 are provided. The three filters 23 are fixed at the bottom of the mounting frame. The filters 23 are interconnected and connected to the high-pressure pump 21 through a hose. The high-pressure pump 21 is connected to the pressure vessel 22 through a hose.

[0051] The stirring mechanism 3 is mainly used for mixing and emulsifying the gel matrix. The stirring mechanism 3 is located on the side of the reverse osmosis mechanism 2 away from the crushing mechanism 1 and is connected to the reverse osmosis mechanism 2. The stirring mechanism 3 includes a stirring tank 31. A vacuum pump 32 is provided on the side of the stirring tank 31 away from the reverse osmosis mechanism 2. An air pipe 33 is provided on the top of the vacuum pump 32. A radiator 34 is connected to the air pipe 33. A filter canister 35 is provided at the bottom of the radiator 34. The air pipe 33 is vertically arranged and located on the side of the vacuum pump 32 away from the stirring tank 31. Two valves connected to the radiator 34 are provided on the air pipe 33. Several screws are provided on the radiator 34.

[0052] The gel preparation is used to improve cervical HPV infection and establish a preventive barrier. The gel preparation is prepared by the following steps: S1, raw material pretreatment: Carbomer 940 is slowly and evenly sprinkled into an appropriate amount of physiological saline and stirred at room temperature to make it fully swell to form a uniform colloid. During the stirring process, care should be taken to avoid the formation of lumps.

[0053] S1.1 Slowly and evenly sprinkle Carbomer 940 into an appropriate amount of physiological saline, stir at room temperature to allow it to fully swell and form a uniform colloid, and take care to avoid clumping during the stirring process;

[0054] S1.2 Add chitosan to a 1% acetic acid solution, stir and dissolve at an appropriate temperature, then remove insoluble matter through a filter to obtain a clear chitosan solution for later use.

[0055] S1.3 Add hyaluronic acid to warm physiological saline, keeping the temperature between 40-50℃. At the same time, add glycerin and polysorbate-80. Use a heating stirrer to stir until all components are fully dissolved and a homogeneous solution is formed. Let the solution cool to room temperature before use.

[0056] S2. Mixing and reaction: Transfer the cooled hyaluronic acid solution to a vacuum emulsifying mixer, turn on the stirring function, and stir at an appropriate speed.

[0057] S2.1 Transfer the cooled hyaluronic acid solution to a vacuum emulsifying mixer, turn on the stirring function, and stir at an appropriate speed;

[0058] S2.2 Add chitosan solution and carbomer colloid in sequence, and stir continuously for 30 minutes to ensure that all components are fully mixed.

[0059] S2.3 Dissolve interferon α-2b in a small amount of physiological saline beforehand. After the above mixture is stirred evenly, slowly add it to the mixture and continue stirring for 15 minutes. Avoid vigorous stirring during the process to prevent the inactivation of interferon α-2b protein.

[0060] S2.4 Use a pH adjustment device to test the pH value of the mixture. When the pH value deviates from the range of 5.5-6.5, use an automatic liquid addition device to accurately add acid or alkali solution to adjust the pH value until it reaches the specified range.

[0061] S2.5 According to the gel viscosity requirements, adjust the gel viscosity to 10000-15000 cP by adding a small amount of physiological saline or carbomer 940 at 25℃. Stir continuously during the adjustment process to ensure uniform viscosity.

[0062] S3. Sterilization treatment: The gel, which is mixed evenly and has been adjusted in pH and viscosity, is filtered through a 0.22μm microporous membrane filter to remove microorganisms and impurities and ensure the sterility of the gel.

[0063] S3.1. The gel, which has been mixed evenly and whose pH value and viscosity have been adjusted, is filtered through a 0.22μm microporous membrane filter to remove microorganisms and impurities, ensuring the sterility of the gel. During the filtration process, pay attention to the pressure changes of the filter to prevent clogging.

[0064] S3.2 In a sterile environment, the filtered gel is precisely filled into medical gel tubes using an aseptic filling machine. Each tube is filled with 5g. The accuracy of the filling volume is controlled by a high-precision metering pump, and the gel tubes are sealed using an automatic sealing function to prevent contamination.

[0065] S4. Finished product quality inspection: On a sterile operating table, take an appropriate amount of finished gel for microbial limit testing. Use incubators, microscopes and other equipment to determine indicators such as total bacterial count and mold count to ensure that it meets the microbial standards stipulated in the pharmacopoeia.

[0066] S4.1 Visually inspect the finished gel; it should be a colorless, transparent, impurity-free, uniformly textured gel-like substance, without layering or discoloration.

[0067] S4.2. Use a pH meter to accurately measure the pH value of the finished gel and ensure that it is within the range of 5.5-6.5; use a rotational viscometer to measure the gel viscosity at 25°C to determine whether it meets the standard requirement of 10000-15000 cP.

[0068] S4.3. Using an osmometer, the osmotic pressure of the gel is measured by the freezing point depression method or vapor pressure method to ensure that it is maintained at 280-310 mOsm / L.

[0069] S4.4 On a sterile operating table, take an appropriate amount of the finished gel for microbial limit testing. Use equipment such as an incubator and microscope to determine indicators such as total bacterial count and mold count to ensure that it meets the microbial standards stipulated in the pharmacopoeia.

[0070] The embodiments disclosed in this invention are preferred embodiments, but are not limited thereto. Those skilled in the art can easily understand the spirit of this invention based on the above embodiments and make different extensions and variations, but as long as they do not depart from the spirit of this invention, they are all within the protection scope of this invention.

Claims

1. A process for the preparation of a gel formulation for improving cervical HPV infection and establishing a preventive barrier, characterized by, Include: Crushing mechanism (1), mainly used for raw material crushing, the crushing mechanism (1) includes cabinet body (11), one side of the cabinet body (11) is provided with cabinet door (12), the crushing barrel (13) is arranged in the cabinet door (12), and the rotating motor (14) matched with the crushing barrel (13) is arranged on the cabinet door (12); Reverse osmosis mechanism (2), mainly used for controlling gel osmotic pressure, the reverse osmosis mechanism (2) is arranged on one side of the crushing mechanism (1) and communicates with the crushing mechanism (1), and the reverse osmosis mechanism (2) comprises a high-pressure pump (21), the high-pressure pump (21) is provided with a pressure container (22), the pressure container (22) is provided with a long tube (24) communicated with the crushing barrel (13), and the bottom of the pressure container (22) is provided with a plurality of filters (23), each filter (23) communicates with the high-pressure pump (21); Stirring mechanism (3), mainly used for mixing and emulsifying gel matrix, the stirring mechanism (3) is arranged on the side, away from the crushing mechanism (1), of the reverse osmosis mechanism (2) and is connected with the reverse osmosis mechanism (2), and the stirring mechanism (3) comprises a stirring barrel (31), one side of the stirring barrel (31), away from the reverse osmosis mechanism (2), is provided with a vacuum pump (32), the top of the vacuum pump (32) is provided with an air pipe (33), the air pipe (33) is connected with a radiator (34), and the bottom of the radiator (34) is provided with a filter tank (35); The gel preparation is applied to improve cervical HPV infection and establish a preventive barrier, and the gel preparation is prepared by the following steps: S1, raw material pretreatment, slowly and uniformly scattering carbomer 940 into appropriate physiological saline, stirring at room temperature, so that it is fully swollen to form a uniform colloid, and attention should be paid to avoid clumping during stirring, and the S1 includes: S1.1, slowly and uniformly scattering carbomer 940 into appropriate physiological saline, stirring at room temperature, so that it is fully swollen to form a uniform colloid, and attention should be paid to avoid clumping during stirring; S1.2, the chitosan is added to 1% acetic acid solution, stirred at an appropriate temperature, then the insoluble substances are removed by a filtering device to obtain a clear chitosan solution, which is ready for use; S1.3, hyaluronic acid is added to warm physiological saline, the temperature is controlled at 40-50 DEG C, glycerol and polysorbate-80 are added at the same time, a heating stirrer is used for stirring, so that the components are fully dissolved to form a uniform solution, and the solution is cooled to room temperature and ready for use; S2, mixing and reaction, the cooled hyaluronic acid solution is transferred to a vacuum emulsifying stirrer, and stirring is started at an appropriate speed, and the S2 includes: S2.1, the cooled hyaluronic acid solution is transferred to a vacuum emulsifying stirrer, and stirring is started at an appropriate speed; S2.2, the chitosan solution and the carbomer colloid are added in sequence, and continuous stirring is carried out for 30 minutes, so that the components are fully mixed and uniform; S2.3, interferon alpha-2b is dissolved in a small amount of normal saline in advance, after the above mixture is stirred uniformly, slowly add it, continue to stir for 15 minutes, avoid violent stirring during stirring, prevent interferon alpha-2b protein inactivation; S2.4, using pH adjusting equipment to detect the pH value of the mixed solution, when the pH value deviates from the range of 5.5-6.5, the automatic liquid adding device is used to accurately add acid or alkali solution for adjustment until the pH value reaches the specified range; S2.5, according to the gel viscosity requirements, by adding a small amount of normal saline or carbomer 940, adjust the gel viscosity to 10000-15000 cP, 25℃, continue to stir during the adjustment process to ensure uniform viscosity; S3, sterilization treatment, the mixed gel with adjusted pH value and viscosity is filtered through a 0.22 μm microporous filter to remove microorganisms and impurities, ensuring the sterility of the gel; S4, product quality detection, take a certain amount of finished gel on the sterile operation table for microbial limit detection, use incubator, microscope equipment to measure total bacterial count and mold quantity index to ensure that it meets the microbial standards specified in the pharmacopoeia.

2. A process for the preparation of a gel formulation for improving cervical HPV infection and establishing a preventive barrier as claimed in claim 1, wherein, The cabinet door (12) is provided with a transparent observation window, and the cabinet door (12) is provided with a handle. The cabinet body (11) is provided with a rotating handle for driving the lifting of the crushing barrel (13), and the side of the cabinet body (11) facing the reverse osmosis mechanism (2) is provided with a mounting groove.

3. A process for the preparation of a gel formulation for improving cervical HPV infection and establishing a preventive barrier as claimed in claim 1, wherein, The high-pressure pump (21) is provided with a mounting bracket at the bottom, and the filter (23) is provided with three, which are all fixed to the bottom of the mounting bracket.

4. The process for the preparation of a gel formulation for improving cervical HPV infection and establishing a preventive barrier as claimed in claim 1 wherein, The several filters (23) are all communicated with each other and connected with the high-pressure pump (21) through a hose, and the high-pressure pump (21) is connected with the pressure container (22) through the hose.

5. The process for the preparation of a gel formulation for improving cervical HPV infection and establishing a preventive barrier as claimed in claim 1 wherein, The air pipe (33) is vertically arranged and placed on the side of the vacuumizing machine (32) away from the stirring barrel (31), the air pipe (33) is provided with two valves connected with the radiator (34), and the radiator (34) is provided with a plurality of screws.

6. A process for the preparation of a gel formulation for ameliorating cervical HPV infection and establishing a preventive barrier as claimed in claim 1, wherein S3 It comprises: S3.1, the mixed gel with adjusted pH value and viscosity is filtered through a 0.22 μm microporous filter to remove microorganisms and impurities, ensuring the sterility of the gel, and the pressure change of the filter is observed during the filtering process to prevent clogging; S3.2, in a sterile environment, the filtered gel is accurately filled into a medical gel tube using a sterile filling machine, each filling capacity is 5g, the accuracy of the filling capacity is controlled by a high-precision metering pump, and the gel tube is sealed by an automatic sealing function to prevent pollution.

7. A process for the preparation of a gel formulation for improving cervical HPV infection and establishing a preventive barrier as claimed in claim 1, wherein S4 It comprises: S4.1, the finished gel is observed by visual observation, which is required to be colorless and transparent, impurity-free, and gel-like material with uniform texture, without stratification and discoloration; S4.2, the pH value of the finished gel is accurately measured by using a pH meter to ensure that it is within the range of 5.5-6.5; the gel viscosity is measured at 25℃ by using a rotary viscometer to determine whether it meets the standard requirement of 10000-15000 cP. S4.3, using osmotic pressure detector, by freezing point depression method or vapor pressure method principle, detection of gel osmotic pressure, ensure that it is maintained at 280-310 mOsm / L; S4.4, in a sterile operating table, take the right amount of finished gel for microbial limit test, using incubator, microscope equipment, determination of total number of colonies, mold quantity index, ensure that it meets the pharmacopoeia of microbial standards.

Images