A rapid ready-to-use gel kit

Abstract

The utility model relates to a kind of quick ready-to-use gel reagent kits applied to gel preparation field, including two glass plates, and the left and right ends of glass plate are inserted with mounting strip, two mounting strips opposite to each other mutually adhere, and the upper end of front mounting strip is carved with slot, slot is equipped with insertion strip, insertion strip is fixedly connected with rear mounting strip, the upper end of rear mounting strip is carved with two limit slots, limit slot is equipped with limit strip, limit strip is fixedly connected with the mounting strip of front, and the upper end of two glass plates is inserted with tooth comb, using the above structure, by the cooperation of insertion strip and slot and limit slot and limit strip, the labyrinth structure is formed between two mounting strips, so that gel is physically cut off, effectively reduce leakage rate, and the mounting strip is extruded and limited by spring self-adapting adjustment to pressing plate, not only simple operation, but also effectively improve the anti-leakage effect, so as to reach the function of quick ready-to-use.

Description

Technical Field

[0001] This invention relates to a gel kit, and more particularly to a rapid, ready-to-use gel kit for use in the field of gel preparation. Background Technology

[0002] Small molecular weight protein precast gels are safe, convenient, and high-quality standard-sized polyacrylamide precast gels mainly used for the detection of small molecular weight proteins. Their electrophoresis buffer system uses the Tris-Tricine electrophoresis buffer system, which can complete electrophoresis in only 60-70 minutes and obtain very flat and sharp bands.

[0003] When preparing gels, the glass plates required for electrophoresis gel preparation consist of a gel plate and a gel concave plate. The gel can only be prepared by perfectly assembling and overlapping the plate and concave plate. The separating gel and stacking gel can only be added by clamping the two plates firmly and using the sponge pad below. However, this operation has certain drawbacks. Leakage often occurs due to the two plates not fitting properly or due to insufficient clamping.

[0004] Chinese patent CN207385507U discloses a magnetic glass plate for gel electrophoresis. The clamping device, first magnetic strip, and second magnetic strip of this utility model work together to completely fit the gel plate and the gel concave plate, avoiding leakage due to insufficient clamping.

[0005] In practical applications, the aforementioned patent uses the combination of magnetism and lead screws to seal the glass plate. However, the magnetic strip sealing method is prone to leakage from the side, and the lead screw pressing requires manual adjustment of multiple lead screws, which is cumbersome and inconvenient for operators. Utility Model Content

[0006] The technical problem that this utility model aims to solve in response to the above-mentioned prior art is that leakage is prone to occur on both sides of the glass plate during gel prefabrication. The existing sealing method uses a combination of screws and magnets to press the two glass plates together, which not only makes the sides of the two glass plates prone to leakage, but also requires operators to manually adjust multiple screws during disassembly and assembly, which is time-consuming, labor-intensive, and inconvenient to use.

[0007] To address the aforementioned problems, this invention provides a rapid, ready-to-use gel reagent kit, comprising two glass plates. Mounting strips are inserted into both ends of the glass plates, with the two opposing mounting strips fitting together. A slot is carved into the upper end of the front mounting strip, and an insert strip is installed within the slot, which is fixedly connected to the rear mounting strip. Two limiting grooves are carved into the upper end of the rear mounting strip, and limiting strips are installed within the limiting grooves, which are fixedly connected to the front mounting strip. A toothed comb is inserted into the upper end of both glass plates, and multiple toothed grooves are carved into the lower end of the comb. A positioning plate is provided at the lower end of both glass plates, and two positioning grooves are carved into the upper end of the positioning plate. A sliding rod is fixedly connected within the positioning grooves. A slider and two springs are movably sleeved on the outer surface of the sliding rod, with the slider positioned between the two springs. The two ends of the springs are fixedly connected to the slider and the inner wall of the positioning groove, respectively. A pressure plate is sleeved around the two fitting mounting strips, and the lower end of the pressure plate is fixedly connected to the upper end of the slider.

[0008] In the aforementioned quick-use gel kit, the use of inserts and slots, as well as limiting grooves and limiting strips, creates a labyrinth structure between the two mounting strips, thereby physically isolating the gel and effectively reducing leakage. The spring-loaded pressure plate adaptively adjusts and squeezes the mounting strips to limit their movement. This not only simplifies operation but also effectively improves the anti-leakage effect, thus achieving the function of quick-use.

[0009] As a further improvement of this application, a sealing plate is fixedly embedded at the upper end of the positioning plate, and the lower end of the glass plate is in contact with the sealing plate.

[0010] As a further improvement of this application, the cross-sections of both the slot and the insert are T-shaped, and the slot and the insert are perfectly matched; the cross-sections of both the limiting groove and the limiting strip are L-shaped, and the limiting groove and the limiting strip are perfectly matched.

[0011] As a further improvement of this application, the longitudinal section of the comb is T-shaped, and the number of tooth grooves is ten or fifteen.

[0012] As another improvement of this application, the pressure plate is U-shaped, and the inner wall of the pressure plate fits against the two adjacent mounting strips.

[0013] As another improvement of this application, one of the glass plates has multiple horizontal scale lines and a vertical calibration line on its surface, the multiple horizontal scale lines are perpendicular to the vertical calibration line, and the surface of the vertical calibration line has scale lines.

[0014] In summary, in practical applications, the two mounting strips can be first fitted onto the left and right ends of the glass plate, respectively. Then, one glass plate is positioned above the other, with the insert aligned with the slot and the limiting strip aligned with the limiting groove. Pressing down on the upper glass plate causes the two adjacent mounting strips to fit together, forming a maze structure that physically isolates the gel and effectively reduces leakage. Next, pull the two pressure plates outward, causing them to move in opposite directions. Place the two glass plates on top of the positioning plate, release the pressure plates to reset them, and then fit them onto the two adjacent mounting strips. This compresses and limits the glass plates and mounting strips, sealing the lower end of the glass plates. Finally, the gel insert comb can be made. During electrophoresis, simply remove the comb upward and the positioning plate downward. This method is not only simple in structure and convenient in operation, but also effectively improves the anti-leakage effect, achieving a quick and ready-to-use function. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural diagram of the first embodiment of this application;

[0016] Figure 2 This is a structural cross-sectional view of the first embodiment of this application;

[0017] Figure 3 This is a schematic diagram of the glass plate structure according to the first embodiment of this application;

[0018] Figure 4 This is a top view of the mounting strip structure according to the first embodiment of this application;

[0019] Figure 5 This is a schematic diagram of the mounting strip structure according to the first embodiment of this application;

[0020] Figure 6 This is a schematic diagram of the positioning plate structure according to the first embodiment of this application;

[0021] Figure 7 This is a front view of the structure of the second embodiment of this application.

[0022] Explanation of the labels in the diagram:

[0023] 1 Glass plate, 2 Mounting strip, 3 Slot, 4 Insert strip, 5 Limiting groove, 6 Limiting strip, 7 Tooth comb, 8 Tooth groove, 9 Positioning plate, 10 Positioning groove, 11 Slide rod, 12 Slider, 13 Spring, 14 Pressure plate, 15 Sealing plate, 16 Horizontal scale line, 17 Vertical calibration line. Detailed Implementation

[0024] The two embodiments of this application will be described in detail below with reference to the accompanying drawings.

[0025] First implementation method:

[0026] Figures 1-3 The diagram shows a rapid, ready-to-use gel kit comprising two glass plates 1, with mounting strips 2 inserted at both ends of the glass plates 1. The two mounting strips 2, which are opposite each other, are attached to each other. A comb 7 is inserted at the upper end of both glass plates 1. The lower end of the comb 7 has multiple grooves 8. The longitudinal section of the comb 7 is T-shaped. The number of grooves 8 is ten or fifteen. When there are many samples to be tested or quantitative analysis is required, the number of grooves 8 can be fifteen, which increases the throughput and facilitates quantitative statistical analysis of more samples. When a very beautiful and representative image is required, the number of grooves 8 can be ten, making the strips obtained by the pre-cast gel smoother and sharper.

[0027] Figures 1-5 As shown: The upper end of the front mounting strip 2 has a slot 3, and the slot 3 contains an insert 4. The insert 4 is fixedly connected to the rear mounting strip 2. The upper end of the rear mounting strip 2 has two limiting grooves 5, and the limiting grooves 5 contain limiting strips 6. The limiting strips 6 are fixedly connected to the front mounting strip 2. The cross-sections of the slot 3 and the insert 4 are both T-shaped, and the slot 3 and the insert 4 are perfectly matched. The cross-sections of the limiting grooves 5 and the limiting strips 6 are both L-shaped, and the limiting grooves 5 and the limiting strips 6 are perfectly matched. The front mounting strip 2 has a T-shaped slot 3, and the rear mounting strip 2 fixes the T-shaped insert 4. After nesting, a horizontal physical barrier is formed. The rear mounting strip 2 has an L-shaped limiting groove 5, and the front mounting strip 2 fixes the L-shaped limiting strip 6. After nesting, a vertical lock is formed, thus creating a maze structure between the two mounting strips 2. Even without external pressure, the leakage rate can be effectively reduced through geometric nesting.

[0028] Figure 2 and Figure 6 The diagram shows that: a positioning plate 9 is provided at the lower end of the two glass plates 1, and two positioning grooves 10 are chiseled at the upper end of the positioning plate 9. A sliding rod 11 is fixedly connected in the positioning groove 10. A slider 12 and two springs 13 are movably sleeved on the outer surface of the sliding rod 11. The slider 12 is located between the two springs 13. The two ends of the springs 13 are fixedly connected to the inner walls of the slider 12 and the positioning groove 10, respectively. A pressure plate 14 is sleeved on the two adjacent mounting strips 2. The pressure plate 14 is U-shaped, and the inner wall of the pressure plate 14 is in contact with the two adjacent mounting strips 2. The lower end of the pressure plate 14 is fixedly connected to the upper end of the slider 12. A sealing plate 15 is fixedly embedded in the upper end of the positioning plate 9. The lower end of the glass plate 1 is in contact with the sealing plate 15. The slider 12 floats on the sliding rod 11. The springs 13 on both sides provide adaptive pressure. The pressure plate 14 sleeves the mounting strips 2, and the slider 12 transmits the evenly distributed pressure, thereby squeezing and limiting the glass plate 1 and the mounting strips 2, effectively avoiding the risk of overload breakage due to manual clamping.

[0029] When making the gel, first, place two mounting strips 2 on the left and right ends of the glass plate 1 respectively, then position one glass plate 1 above the other, aligning the insert 4 with the slot 3 and the limiting strip 6 with the limiting groove 5. Press down on the upper glass plate 1 to make the two adjacent mounting strips 2 fit together, forming a maze structure between the two mounting strips 2, thus physically isolating the gel and effectively reducing the leakage rate. Then, pull the two pressure plates 14 outwards, causing the two pressure plates 14 to move in opposite directions. At this time, the two springs 1 on the outer side... All three components are compressed outwards, and the spring 13 inside the slider 12 is stretched outwards, placing the two glass plates 1 on the upper end of the positioning plate 9. The pressure plate 14 is released to reset it and is fitted onto the two adjacent mounting strips 2, thereby squeezing and limiting the glass plates 1 and the mounting strips 2, sealing the lower end of the glass plates 1. Finally, the gel insert comb 7 is made. During electrophoresis, it is only necessary to remove the comb 7 upwards and the positioning plate 9 downwards. Not only is the structure simple and the operation convenient, but it also effectively improves the anti-leakage effect, thus achieving the function of quick and ready-to-use.

[0030] Second implementation method:

[0031] This embodiment adds a horizontal scale line 16 and a vertical calibration line 17 to the first embodiment, while the rest remains the same as the first embodiment.

[0032] Figure 7 As shown: On the surface of one of the glass plates 1, there are multiple horizontal scale lines 16 and a vertical calibration line 17. The multiple horizontal scale lines 16 are perpendicular to the vertical calibration line 17, and the surface of the vertical calibration line 17 is provided with scale lines.

[0033] During electrophoresis, the electrophoretic position can be more easily determined by multiple horizontal graduation lines 16 and a vertical calibration line 17 on the glass plate 1, so that the same dye can run to the same position in different gels. This increases the comparability between different gel plates, improves the accuracy of band analysis, and effectively reduces the error during analysis.

[0034] In light of current practical needs, the above-described embodiments adopted in this application are not limited to these. Any changes made within the scope of knowledge possessed by those skilled in the art without departing from the concept of this application still fall within the protection scope of this utility model.

Claims

1. A rapid, ready-to-use gel reagent kit, comprising two glass plates (1), characterized in that: The glass plate (1) has mounting strips (2) inserted at both ends. The two mounting strips (2) that are opposite each other are fitted together. The upper end of the front mounting strip (2) has a slot (3) with a insert (4) inside. The insert (4) is fixedly connected to the rear mounting strip (2). The upper end of the rear mounting strip (2) has two limiting grooves (5) with limiting strips (6) inside. The limiting strips (6) are fixedly connected to the front mounting strip (2). The upper ends of the two glass plates (1) are jointly fitted with a toothed comb (7). The lower end of the toothed comb (7) has multiple toothed grooves (8). The lower ends of the two glass plates (1) are provided with positioning plates (9). The upper ends of the positioning plates (9) are chiseled with two positioning grooves (10). A sliding rod (11) is fixedly connected in the positioning grooves (10). A slider (12) and two springs (13) are movably sleeved on the outer surface of the sliding rod (11). The slider (12) is located between the two springs (13). The two ends of the springs (13) are fixedly connected to the inner walls of the slider (12) and the positioning grooves (10), respectively. A pressure plate (14) is sleeved on the two fitting mounting strips (2). The lower end of the pressure plate (14) is fixedly connected to the upper end of the slider (12).

2. The rapid, ready-to-use gel kit according to claim 1, characterized in that: The upper end of the positioning plate (9) is fixedly inlaid with a sealing plate (15), and the lower end of the glass plate (1) is in contact with the sealing plate (15).

3. The rapid, ready-to-use gel reagent kit according to claim 1, characterized in that: The slot (3) and the insert (4) are both T-shaped in cross-section, and the slot (3) and the insert (4) are perfectly matched. The limiting groove (5) and the limiting strip (6) are both L-shaped in cross-section, and the limiting groove (5) and the limiting strip (6) are perfectly matched.

4. The rapid, ready-to-use gel reagent kit according to claim 1, characterized in that: The longitudinal section of the comb (7) is T-shaped, and the number of the tooth grooves (8) is ten or fifteen.

5. The rapid, ready-to-use gel kit according to claim 1, characterized in that: The pressure plate (14) is U-shaped, and the inner wall of the pressure plate (14) is in contact with the two adjacent mounting strips (2).

6. The rapid, ready-to-use gel kit according to claim 1, characterized in that: One of the glass plates (1) has multiple horizontal scale lines (16) and a vertical calibration line (17) on its surface. The multiple horizontal scale lines (16) are perpendicular to the vertical calibration line (17), and the surface of the vertical calibration line (17) is provided with scale lines.

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