Antioxidant repairing composition, application and cosmetic

Abstract

The invention discloses an antioxidant repairing composition and application thereof and cosmetics, the antioxidant repairing composition comprises the following components in parts by mass: 32-48 parts of a Chinese herbaceous peony extract combined factor, 8-12 parts of a radix sophorae flavescentis extract combined factor, 1.6-2.4 parts of zinc gluconate and 1.6-2.4 parts of a fibronectin factor, through the mutual cooperation of the four components, the antioxidant effect of the skin can be effectively improved, the damage of oxidative stress and inflammation to the skin can be effectively reduced, and the cell regeneration effect of the skin is promoted; belongs to the technical field of cosmetics.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to an antioxidant repairing composition and its use, and cosmetics. Background Art

[0002] Oxidative stress plays a crucial role in the aging process of the skin. Oxidative stress occurs when the production of free radicals (particularly reactive oxygen species (ROS)) in the body exceeds the capacity of the body's natural antioxidant defense system. This can lead to DNA damage, increased expression of multiple pro-inflammatory factors such as tumor necrosis factor-α, interleukin 1, interleukin 6, and interleukin 8, a decrease in the skin's own antioxidants, and the production of matrix metalloproteinases (MMPs). MMPs can degrade collagen and elastin in the dermis. Inflammatory responses mediate skin damage, leading to impaired barrier function. A damaged barrier makes it easier for ROS to attack the skin, further exacerbating skin aging and barrier damage.

[0003] Antioxidant repair cosmetics currently on the market typically contain active ingredients such as vitamin C, vitamin E, and polyphenols. These ingredients can effectively neutralize free radicals in the skin. By reducing free radical levels, these products can, to a certain extent, combat oxidative stress, thereby helping to improve skin quality and slow the aging process. However, relying solely on reducing free radicals to repair skin problems has certain limitations, as skin health is not only affected by free radicals but also by other factors, such as the natural aging process. Therefore, relying solely on reducing free radicals to repair skin will result in repair effects that fail to meet consumer expectations.

[0004] The problem that this proposal needs to solve is: how to propose a new antioxidant repair composition. Summary of the Invention

[0005] To this end, the first purpose of the present invention is to provide an antioxidant repair composition that can effectively improve the antioxidant effect of the skin, reduce the damage to the skin caused by oxidative stress and inflammation, and promote skin cell regeneration.

[0006] Another object of the present invention is to provide the use of the antioxidant repair composition, specifically as a cosmetic additive.

[0007] Another object of the present invention is to provide an antioxidant and repairing cosmetic.

[0008] To this end, the first technical solution provided by the present invention is as follows: An antioxidant repair composition comprises the following active components in parts by mass: 32 to 48 parts of a peony extract combination factor, 8 to 12 parts of a sophora flavescens root extract combination factor, 1.6 to 2.4 parts of zinc gluconate, and 1.6 to 2.4 parts of a fibronectin factor.

[0009] Furthermore, the above antioxidant repair composition also contains 32-48 parts of water, 4-6 parts of butanediol, 0.4-0.6 parts of 1,2-hexanediol, and 0.4-0.6 parts of p-hydroxyacetophenone.

[0010] Furthermore, in the above-mentioned antioxidant repair composition, the peony extract combination factor is calculated based on the total mass as 100%, including the following components and their mass percentages: peony root extract 25.0%, butylene glycol 50.0%, water 23.50%, 1,2-hexanediol 1.0% and parahydroxyacetophenone 0.5%.

[0011] Furthermore, in the above-mentioned antioxidant repair composition, the Sophora flavescens root extract combination factor is calculated based on the total mass as 100%, including the following components and their mass percentages: 69.0% water; 20.0% butylene glycol; 10.0% Sophora flavescens root extract; 0.5% 1,2-hexanediol and 0.5% parahydroxyacetophenone.

[0012] Furthermore, in the above antioxidant repair composition, the fibronectin factor, calculated based on the total mass 100%, includes the following components and their mass percentages: 90.0% mannitol and 10.0% fibronectin.

[0013] Another technical solution of the present invention is to provide the use of the antioxidant repair composition described in the first technical solution as a cosmetic additive.

[0014] Another technical solution of the present invention is to provide a cosmetic comprising 1 to 20 wt % of the antioxidant repair composition described in the first technical solution.

[0015] Preferably, the cosmetic contains 2.5-20 wt% of the antioxidant repair composition described in the first technical solution.

[0016] Preferably, the cosmetic is one of lotion, emulsion, cream, essence, facial mask, cleanser and shower gel.

[0017] The beneficial effects of the present invention are: (1) The paeoniflorin, matrine, and polyphenols in the peony extract and sophora flavescens extract of the present invention can directly remove reactive oxygen species (ROS) and hydroxyl radicals (OH -), blocking free radical chain reactions and alleviating oxidative stress damage to biomacromolecules such as lipids and proteins. It also regulates signaling pathways such as NF-κB, inhibiting the release of inflammatory factors, reducing oxidative stress and inflammatory damage, and accelerating wound healing. It also activates fibroblasts and stimulates collagen production, improving skin elasticity and reducing wrinkle formation. Zinc gluconate provides zinc ions to enhance cell membrane repair, cell proliferation, growth, and immune system function, ensuring that cell membrane repair maintains cell integrity and safeguards normal cell metabolism and function. Cell proliferation helps increase the number of cells at the wound site and promotes new tissue formation. A properly functioning immune system can resist infection at the wound site and create a favorable environment for wound healing. These three ingredients work together to effectively enhance the skin's antioxidant capacity, reduce the damage caused by oxidative stress and inflammation, and promote cell regeneration.

[0018] (2) In the technical solution provided by the present invention, the paeoniflorin in the peony extract and the oxymatrine in the sophora flavescens can enhance the activity of endogenous antioxidant enzymes by scavenging ROS and activating the Nrf2 / ARE pathway, thereby achieving multi-target antioxidant effect; zinc gluconate inhibits the NF-κB signaling pathway and works together with the flavonoid components of the sophora flavescens extract to downregulate the expression of pro-inflammatory factors such as IL-6 and TNF-α, thereby achieving anti-inflammatory synergy; the composition can accelerate barrier repair by upregulating the synthesis of EGFR and Collagen IV in keratinocytes, thereby promoting cell regeneration.

[0019] (3) The technical solution provided by the present invention combines fibronectin with peony extract, Sophora flavescens extract and zinc gluconate. Fibronectin activates the FAK / PI3K pathway through integrin α5 and β1 receptors, and synergizes with peony extract to promote the migration, oxidative damage repair and proliferation of fibroblasts; the RGD sequence of fibroblasts and zinc gluconate jointly stabilize the mitochondrial membrane potential, reduce H2O2-induced cell apoptosis, enhance the migration ability of human keratinocytes, and repair oxidatively damaged skin; fibronectin upregulates claudin-1 expression, and synergizes with Sophora flavescens extract to enhance the integrity of tight junctions, repair and strengthen the skin barrier function; thereby achieving a synergistic enhancement of antioxidant repair efficacy. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The photographs show the effects of different concentrations of the test samples in Example 1 on HaCaT cell scratches; Figure 2 This is a bar graph showing the effects of different concentrations of the test samples on HaCaT cell scratches in Example 1; Figure 3 These are photos showing the effects of different concentrations of the test samples in Example 1 on HSF cell scratches; Figure 4 This is a bar graph showing the effects of different concentrations of the test samples on HSF cell scratching in Example 1; Figure 5 The figure is a bar graph showing the clearance rates of superoxide anions by the test samples of different concentrations in Example 1; Figure 6 This is a bar graph showing the effect of 0.25% of the tested samples on the SOD gene expression in HaCaT cells; Figure 7 The figure is a bar graph showing the effect of 0.25% of the tested samples on the expression of CAT gene in HaCaT cells. DETAILED DESCRIPTION

[0021] Below in conjunction with embodiments of the present invention, technical scheme of the present invention is clearly and completely described, obviously, described embodiment is only a part of embodiment of the present invention, rather than whole embodiment. Based on the embodiment in the present invention, those of ordinary skill in the art, without making the every other embodiment obtained under creative work premise, all fall within the scope of protection of the present invention. It should be noted that, in the embodiment, those not indicating specific conditions, carry out according to the condition of normal condition or manufacturer's suggestion. Reagents used or instrument not indicating manufacturer, all are conventional products that can be obtained by commercial purchase.

[0022] In the present invention, unless otherwise specified, the following "Sanmusin" is the trade name of the antioxidant repair composition provided by this application.

[0023] The commodity information of materials in the following examples is as follows: The peony extract combination factors were purchased from Shanghai Zhina Biotechnology Co., Ltd. The combined factors of Sophora flavescens extract were purchased from Shanghai Zhina Biotechnology Co., Ltd. Fibronectin factor was purchased from Guangzhou Jikemei Life Science Co., Ltd.; Zinc gluconate was purchased from Zhengzhou Ruipu Bioengineering Co., Ltd.

[0024] The components of the peony extract combination factor, Sophora flavescens extract combination factor, and fibronectin factor used in the following examples are shown in Tables 1 to 3.

[0025] Table 1 Combination factors of peony extract

[0026] Table 2 Combination factors of Sophora flavescens extracts

[0027] Table 3 Fibronectin factor

[0028] Example 1

[0029] This embodiment provides an antioxidant repair composition, which, calculated based on 100% total mass, includes the following components and contents: 2.0% fibronectin factor; 40.0% peony extract combination factor; 10.0% sophora flavescens extract combination factor; 2.0% zinc gluconate; 40.0% water; 5.0% butylene glycol; 0.5% 1,2-hexanediol; and 0.5% parahydroxyacetophenone.

[0030] Example 2

[0031] This embodiment provides an antioxidant repair composition, which, calculated based on 100% total mass, includes the following components and contents: 2.40% fibronectin factor; 48.0% peony extract combination factor; 8.0% sophora flavescens extract combination factor; 1.60% zinc gluconate; 35.0% water; 4.0% butylene glycol; 0.60% 1,2-hexanediol; and 0.40% parahydroxyacetophenone.

[0032] Example 3

[0033] This embodiment provides an antioxidant repair composition, which, calculated based on 100% total mass, includes the following components and contents: 1.60% fibronectin factor; 32.0% peony extract combination factor; 12.0% sophora flavescens extract combination factor; 2.40% zinc gluconate; 45.0% water; 6.0% butanediol; 0.40% 1,2-hexanediol; and 0.60% parahydroxyacetophenone.

[0034] Example 4

[0035] This embodiment provides an antioxidant repair composition, which, calculated based on 100% total mass, includes the following components and their contents: 2.20% fibronectin factor; 36.0% peony extract combination factor; 9.0% sophora flavescens extract combination factor; 2.40% zinc gluconate; 45.0% water; 4.20% butylene glycol; 0.60% 1,2-hexanediol; and 0.60% parahydroxyacetophenone.

[0036] Comparative Examples 1-4 Comparative Examples 1-4 provide an antioxidant repair composition comprising the components and mass percentages shown in Table 4.

[0037] Table 4

[0038] Performance testing: 1. Repair performance test 1 Experimental Principle Cell migration refers to the movement of cells in response to a migration signal or stimulation by a substance. Increased cell migration ability indicates a repair-promoting effect. A blank area, called a "scratch / wound," is artificially created on a confluent cell monolayer. Cells at the edge of the scratch gradually migrate into the blank area, healing the scratch / wound. Images are captured periodically at the onset and during cell migration. By measuring the distance between the scratches at different time points and calculating the difference, the cell's migration ability can be assessed.

[0039] 2 Materials and Equipment 2.1 Cell lines: HaCaT (human immortalized keratinocytes, American Type Culture Collection (ATCC CRL-2404)), HSF (human skin fibroblasts, purchased from Probio (Shanghai) Biotechnology Co., Ltd.); 2.2 Equipment: CO2 incubator (Thermo, 150I), clean bench (Sujing Antai, SW-CJ-1F), Olympus CK 53 microscope, multifunctional microplate reader (TECAN, SPARK); 2.3 Reagents: MTT (thiazolyl blue), DMSO (dimethyl sulfoxide), cell culture medium (DMEM), EGF (4800 IU / bottle, China National Institutes for the Control of Biological Products), double-antibody.

[0040] 3. Processing of tested samples Test sample group: The antioxidant repair compositions prepared in Examples 1-4 and Comparative Examples 1-4 were filtered through a 0.22 μm filter, and the filtrates were collected as the test sample mother liquors, which were then diluted with purified water to a volume concentration of 0.05%, 0.1%, and 0.2%; Blank control group: (pure water); Positive control group: EGF (10 ng / ml).

[0041] 4 Experimental methods 4.1 Cell viability test Cells in good growth condition were plated on 96-well plates and cultured for 24 hours. The culture medium was then replaced with the test samples at different volume concentrations (0.05%, 0.1%, and 0.2%). After 24 hours, the OD570 and OD630 values ​​were detected by MTT method to analyze the effects of the test samples on cell tolerance.

[0042] 4.2 Cell migration assay Take cells in good growth condition and spread them on a 12-well plate. After 24 hours, wait for the cells to cover the bottom. Use a 200μL pipette tip to gently scratch the bottom to simulate human skin damage. Discard the culture medium and gently rinse the detached cells with PBS. Then add culture medium containing different concentrations of the test sample. At the same time, set up a blank control and a positive control (10ng / mL EGF). Observe and take pictures under a microscope at 0h, 12h, and 24h. Figure 1 、 Figure 3 The scratch area was measured using ImageJ software as shown. Figure 2 、 Figure 4 , the scratch healing rate was calculated according to Formula 1: Formula 1: Scratch healing rate P = (scratch area at 0 h - scratch area at each time point) / scratch area at 0 h × 100% The times in formula 1 are 12 hours and 24 hours.

[0043] 5 Experimental Results 5.1 Calculation data of HaCaT cell scratch healing rate are shown in Tables 5 and 6.

[0044] Table 5 HaCaT cell scratch healing rate Blank control EGF (10 ng / ml) Sample 0.05% Sample 0.1% Sample 0.2% 0h 0 0 0 0% 0% 12h 33.5% 40.96% 34.82% 36.52% 36.95% 24h 70% 91.25% 79% 88% 90% Note: Sample 0.05% refers to the test sample containing 0.05% (v / v) of the antioxidant repair composition provided in Example 1; Sample 0.1% (v / v) refers to a test sample containing 0.1% of the antioxidant repair composition provided in Example 1; The sample 0.2% (v / v) refers to the test sample containing 0.2% of the antioxidant repair composition provided in Example 1.

[0045] By 1 and Figure 2 As can be seen from Table 5, after treating HaCaT cells with 0.05%, 0.1% and 0.2% Trimusin (Example 1) for 24 hours, the wound healing rates were 79%, 88% and 90%, respectively, which were significantly different from those of the blank control group (P<0.05, P<0.0%1, P<0.0%1).

[0046] It can also be seen that when the antioxidant repair composition provided in Example 1 was present at a concentration of 0.2%, its HaCaT cell wound healing rate was the highest. Therefore, the HaCaT cell wound healing rates after treating HaCaT cells with the compositions provided in Examples 2-4 and Comparative Examples 1-4 at a concentration of 0.2% by volume for 24 hours are shown in Table 6.

[0047] For ease of recording, the compositions provided in Examples 2-4 and Comparative Examples 1-4 are respectively recorded as Sample 2, Sample 3, Sample 4, Comparative Sample 1, Comparative Sample 2, Comparative Sample 3 and Comparative Sample 4.

[0048] Table 6 Sample 1 Sample 2 Sample 3 Sample 4 Comparative Sample 1 Comparative Sample 2 Comparative Sample 3 Comparative Sample 4 0h 0% 0% 0% 0% 0% 0% 0% 0% 12h 36.95% 36.41% 37.26% 40.36% 28.31% 32.91% 30.04% 22.14% 24h 90% 92.4% 91.2% 93.65% 70.23% 84.11% 81.3% 61.88% It can be seen that the technical solution provided in this application can significantly improve the migration ability and repair efficiency of cells; compared with the control samples, the cells treated with the antioxidant repair composition provided in Examples 2-4 have a higher scratch healing rate within the same period of time.

[0049] 5.2 The calculation results of HSF cell scratch healing rate are shown in Tables 7 and 8.

[0050] Table 7 HSF cell scratch healing rate

[0051] Through 2 and Figure 4 As can be seen from Table 7, after HSF cells were treated with 0.1% and 0.2% triamcinolone for 12 hours, the wound healing rates were 38.40% and 39.88%, respectively, which were statistically significant compared with the blank control group (P<0.05). After HSF cells were treated with 0.1% and 0.2% triamcinolone for 24 hours, the wound healing rates were 91.52% and 93.27%, respectively, which were significantly different compared with the blank control group (P<0.01).

[0052] It can also be seen that when the antioxidant repair composition provided in Example 1 was present at a concentration of 0.2%, its HaCaT cell wound healing rate was the highest. Therefore, the HSF cell wound healing rates after treating HSF cells with the compositions provided in Examples 2-4 and Comparative Examples 1-4 at a concentration of 0.2% by volume for 24 hours are shown in Table 8.

[0053] For ease of recording, the compositions provided in Examples 2-4 and Comparative Examples 1-4 are respectively recorded as Sample 2, Sample 3, Sample 4, Comparative Sample 1, Comparative Sample 2, Comparative Sample 3 and Comparative Sample 4.

[0054] Table 8 HSF cell scratch healing rate

[0055] In summary, Sanmusin can significantly promote the migration of HaCaT human immortalized keratinocytes and HSF human skin fibroblasts, and has the effect of promoting skin cell regeneration.

[0056] 2. Antioxidant Performance Test 1 Experimental Background 1.1 Superoxide anion (O2- ) Superoxide anion is a free radical produced during normal metabolic processes, playing a variety of physiological roles in the body, including signal transduction. Superoxide anion can be converted into other oxygen free radicals through reactions and is a precursor to all other oxygen free radicals. Excessive levels can damage cellular structures. Therefore, superoxide anion free radical scavenging ability is an important indicator when evaluating the antioxidant properties of test substances.

[0057] 1.2 Superoxide dismutase (SOD) and catalase (CAT) SOD and CAT are important components of the body's antioxidant system. When cells are stimulated by external stimuli, a large number of free radicals are produced within the cell. SOD is then activated to convert superoxide anion radicals into hydrogen peroxide and oxygen. CAT then decomposes the harmful hydrogen peroxide into water and oxygen. In most cases, if the test substance can effectively protect against UV oxidative damage (such as an antioxidant), it will reduce the cell's dependence on antioxidant enzymes such as SOD and CAT, thereby downregulating SOD and CAT gene expression. If the test substance activates the cell's own antioxidant defense system (such as an Nr2 agonist), it may directly upregulate SOD and CAT gene expression.

[0058] 2 Equipment and Materials 2.1 Equipment: Multifunctional microplate reader (TECAN, SPARK), water bath, CO2 incubator (Thermo), fluorescence quantitative PCR instrument (Bio-Rad, CFX Conmeet Ootics odule), PCR amplifier (Longji, A300); 2.2 Cell line: HaCaT (human immortalized keratinocytes, American Type Culture Collection (ATCC CRL-2404)); 2.3 Reagents: Vitamin C (ascorbic acid, purity ≥98, source leaf), Tris (hydroxymethylaminomethane) (GBCBIO Technologies Inc), pyrogallol (McLean), concentrated hydrochloric acid (analytical grade, Guangzhou Chemical Reagent), RNA rapid extraction kit (Meiji Biotechnology), qPCR Mix kit (SYBR Green); 3. Sample processing The compositions prepared in Examples 1-4 and Comparative Examples 1-4 were diluted in sequence according to a concentration gradient.

[0059] 4 Test methods 4.1 Superoxide anion scavenging assay The sample to be tested was prepared in double-distilled water to a gradient of different concentrations (0, 1.0, 2.5, 5, 10, 15, 20 (v / v, %)). Tris-HCl buffer (0.05 mol / L) was added to each centrifuge tube. The tube was preheated in a 25°C water bath. The sample solution at different concentrations was then added. Pyrogallol solution (0.2 mmol / L) was then added and mixed thoroughly. The mixture was then reacted in a 25°C water bath. The reaction was immediately terminated with two drops of concentrated hydrochloric acid, and the absorbance was measured at a wavelength of 325 nm. Three replicates were performed for each tube, and the average value was taken. The superoxide anion scavenging rate was calculated according to Equation 2.

[0060] Formula 2: O2 - Clearance rate (%) = (A0-A T ) / A0×100; Where, A0-absorbance value of solvent tube; A T -Absorbance value of sample tube; GraphPad Prism 8.0 was used for statistical analysis, and one-way analysis of variance was performed for comparisons among multiple groups.

[0061] 4.2 HaCaT cell activity test HaCaT cells in good growth condition were plated on 96-well plates and cultured for 24 hours. The culture medium was then replaced with the culture medium containing different concentrations of the test samples. After 24 hours, the OD450 value was detected by CCK-8 method to analyze the effect of the test samples on the tolerance of HaCaT cells.

[0062] 4.3 Antioxidant-related enzyme gene assay HaCaT cells were resuspended in fresh complete culture medium and seeded into 24-well plates.

[0063] UV stimulation: discard the old culture medium, add a small amount of PBS to each well, and perform UVB irradiation stimulation with a total irradiation intensity of 48mJ / cm².

[0064] Induction and administration: After irradiation stimulation, discard the PBS in the 24-well plate, add the prepared test sample, and place the 24-well plate in a CO2 incubator for 48 hours. After the incubation, collect the cells to extract total mRNA for quantification, and reverse transcribe to obtain cDNA for Real-Time PCR reaction. -△△Ct The relative expression of SOD and CAT genes was analyzed by the method. The average Ct value of three replicates of each gene in each cDNA sample was used as the amplification result. The amplification amount of GAPDH gene was used as the internal reference gene. The gene cycle threshold △C=Ct gene-Ct GAPDH, the relative expression of gene △△Ct=△Ct test substance-△Ct blank control, was calculated with 2 -△△Ct The expression ratio between the test group and the control group was analyzed and calculated.

[0065] 5 Experimental Results 5.1 Superoxide anion (O2 - ) The clearance test results are shown in Table 9.

[0066] Table 9 Superoxide anion clearance data

[0067] Combined with Table 9 and Figure 5 It can be seen that when the composition prepared in Example 1 is used, the composition provided in Example 1 with a volume concentration of 2.5% to 20.0% has a significant effect on the superoxide anion (O2 - ) showed a significant scavenging effect (P < 0.05), demonstrating excellent antioxidant efficacy. The scavenging rate was concentration-dependent, ranging from 10.46% to 112.05%. Furthermore, as the concentration of the composition in the sample increased, its superoxide anion scavenging rate also increased, reaching 112.05% at a 20% volume concentration.

[0068] Further measurements were performed on the compositions provided in Examples 2-3 and Comparative Examples 1-4 at a volume concentration of 20%, and the results are shown in Table 10.

[0069] For ease of recording, the compositions provided in Examples 2-4 and Comparative Examples 1-4 are respectively recorded as Sample 2, Sample 3, Sample 4, Comparative Sample 1, Comparative Sample 2, Comparative Sample 3 and Comparative Sample 4.

[0070] Table 10

[0071] It can be seen from Table 10 that when the volume concentration of the composition of Example 2 is 20%, its superoxide anion scavenging rate is the highest, reaching 122.07±1.97, showing the strongest antioxidant performance; the superoxide anion scavenging rates of the compositions provided by Comparative Examples 1-4 are significantly lower than the superoxide anion scavenging rates of the compositions provided in the Examples.

[0072] 5.3 Antioxidant-related enzyme gene expression The effects of the tested samples on the expression of SOD gene in HaCaT cells are shown in Table 11 and Figure 6 The effects of the tested samples on CAT gene expression in HaCaT cells are shown in Tables 12 and Figure 7 .

[0073] Among them: Blank group: deionized water Positive control group: Vitamin C (ascorbic acid, purity ≥98%, source leaf) Sample 1: 0.25 (v / v)% of the composition provided in Example 1 Table 11 Effects of the tested samples on SOD gene expression in HaCaT cells

[0074] Through Table 11 and Figure 6 It can be seen that sample 1*P<0.05; positive control group***P<0.001 (Note: * indicates that the tested sample has a significant difference compared with the model group at this concentration), proving that the experimental system is effective.

[0075] Table 12 Effects of the tested samples on CAT gene expression in HaCaT cells

[0076] Through Table 12 and Figure 7 It can be seen that sample 1*P<0.05; positive control group**P<0.01 (Note: * indicates that the tested sample has a significant difference compared with the model group at this concentration), proving that the experimental system is effective.

[0077] In summary, 0.25% Sanmusin has an inhibitory effect on the increased expression of intracellular SOD and CAT enzyme genes caused by ultraviolet oxidative damage (P<0.05).

[0078] Example 5 This embodiment provides a lotion, which is composed of 1 wt % of the antioxidant repairing composition described in Example 1 and 99 wt % of a conventional lotion base.

[0079] Example 6 This embodiment provides an emulsion, which is composed of 5 wt % of the antioxidant repair composition described in Example 2 and 95 wt % of a conventional emulsion matrix.

[0080] Example 7 This embodiment provides an essence, which is composed of 20 wt % of the antioxidant repair composition described in Example 3 and 80 wt % of a conventional essence matrix.

[0081] Example 8 This embodiment provides a facial mask, which is composed of 2.5 wt % of the antioxidant repair composition described in Example 4 and 97.5 wt % of a conventional facial mask matrix.

[0082] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principles of the present invention should be considered as equivalent replacement methods and are included in the scope of protection of the present invention.

Claims

1. An antioxidant repair composition, characterized in that, Calculated by mass, the active components include: 32 to 48 parts of peony extract combination factors, 8 to 12 parts of sophora flavescens root extract combination factors, 1.6 to 2.4 parts of zinc gluconate, and 1.6 to 2.4 parts of fibronectin factors.

2. The antioxidant repair composition according to claim 1, characterized in that It also contains 32-48 parts of water, 4-6 parts of butanediol, 0.4-0.6 parts of 1,2-hexanediol, and 0.4-0.6 parts of p-hydroxyacetophenone.

3. The antioxidant repair composition according to claim 1, characterized in that The peony extract combination factor is calculated based on 100% of the total mass, and includes the following components and their mass percentages: 25.0% of peony root extract, 50.0% of butylene glycol, 23.50% of water, 1.0% of 1,2-hexanediol and 0.5% of p-hydroxyacetophenone.

4. The antioxidant repair composition according to claim 1, characterized in that The combination factor of the Sophora flavescens root extract is calculated based on 100% of the total mass, and includes the following components and their mass percentages: 69.0% water; 20.0% butanediol; 10.0% Sophora flavescens root extract; 0.5% 1,2-hexanediol and 0.5% p-hydroxyacetophenone.

5. The antioxidant repair composition according to claim 1, characterized in that The fibronectin factor is calculated based on 100% of the total mass and includes the following components and their mass percentages: 90.0% of mannitol and 10.0% of fibronectin.

6. Use of the antioxidant repairing composition according to any one of claims 1 to 5 as a cosmetic additive.

7. A cosmetic, characterized in that: The cosmetic contains 1 to 20 wt % of the antioxidant repair composition according to any one of claims 1 to 5.

8. The cosmetic according to claim 7, characterized in that The cosmetic contains 2.5-20 wt % of the antioxidant repair composition according to any one of claims 1-5.

9. The cosmetic according to claim 8, characterized in that The cosmetic is one of lotion, emulsion, cream, essence, facial mask, cleanser and shower gel.

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