Radix bupleuri slice and preparation method thereof

Abstract

The present application relates to a kind of bupleurum decoction piece and its preparation method, it is related to bupleurum decoction piece technical field, the preparation method of the bupleurum decoction piece includes the following steps: fresh clean bupleurum root is sliced, and then low-temperature plasma treatment, obtain pretreatment bupleurum piece;The pretreatment bupleurum piece is dried using magnetic field heat treatment method, obtain the bupleurum decoction piece.Compared with traditional bupleurum decoction piece preparation process, the present application innovatively introduces low-temperature plasma technology and magnetic field heat treatment technology, not only significantly improves the content of effective components such as saikosaponin in the obtained bupleurum decoction piece and its quality stability, effectively reduces the risk of aflatoxin during storage;And the present application omits the time-consuming dampening process in traditional process, greatly improves the preparation efficiency, provides a new process for the processing production of bupleurum decoction piece.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of Bupleurum decoction pieces, and in particular to a Bupleurum decoction piece and a preparation method thereof. BACKGROUND

[0002] As a traditional and commonly used Chinese medicinal material, Bupleurum occupies an important position in the field of traditional Chinese medicine. It is cold in nature, bitter and acrid in taste, and belongs to the liver and gallbladder meridians. It has the effects of dispersing heat, soothing the liver and relieving depression, and raising yang qi, and is widely used in the treatment of colds, fever, alternating chills and fever, chest and abdominal pain, irregular menstruation, uterine prolapse, and anal prolapse. In clinical application, Bupleurum is often used in the form of decoction pieces, so the quality of Bupleurum decoction pieces directly affects its clinical efficacy and safety.

[0003] The preparation process of traditional Bupleurum decoction pieces mainly includes the steps of cleaning, drying, washing, cutting, and drying. Among them, the washing step usually adopts the method of steaming, that is, the washed Bupleurum roots are placed in a certain humidity and temperature condition for about 20 hours to absorb water naturally to achieve the purpose of softening. However, this traditional process has many disadvantages as follows:

[0004] 1. From the aspect of effective ingredient content, the traditional steaming process is prone to cause the loss of effective ingredients in Bupleurum due to the long processing time and the difficulty in accurately controlling the environmental conditions, which may cause some effective ingredients in Bupleurum, such as saikosaponin, to undergo chemical reactions such as hydrolysis and oxidation.

[0005] 2. In terms of quality stability, the traditional preparation process also faces challenges. During the steaming process, Bupleurum roots are in a relatively humid environment, which provides favorable conditions for the growth and reproduction of microorganisms. If the hygiene conditions are not well controlled during the steaming process, mold and other microorganisms are likely to breed, which not only affects the appearance and odor of Bupleurum decoction pieces, but also may continue to reproduce during the subsequent storage process, leading to the deterioration of the decoction pieces.

[0006] 3. The steaming process in the traditional process takes a long time, which greatly prolongs the preparation period of Bupleurum decoction pieces, reduces the production efficiency, and increases the production cost. In the context of the rapid development of the traditional Chinese medicine industry and the increasing demand for the quality and production efficiency of traditional Chinese medicine, the traditional preparation process of Bupleurum decoction pieces has been difficult to meet the market demand. SUMMARY

[0007] To solve the above problems, the present application provides a Bupleurum decoction piece and a preparation method thereof.

[0008] In a first aspect, the present application provides a preparation method of a Bupleurum decoction piece, which comprises the following steps:

[0009] The fresh and clean Bupleurum roots are sliced, and then subjected to low-temperature plasma treatment to obtain pretreated Bupleurum pieces;

[0010] The pre-processed Bupleurum root slices are dried by adopting a magnetic field heat treatment mode to obtain the Bupleurum root slices;

[0011] The magnetic field heat treatment comprises the following processes:

[0012] The first magnetic field heat treatment is performed at a magnetic field intensity of 0.1-0.4 T and a temperature of 45-50 DEG C for 30-60 minutes.

[0013] The second magnetic field heat treatment is performed at a magnetic field intensity of 0.6-1.0 T and a temperature of 60-65 DEG C for 60-90 minutes.

[0014] Further, the working condition parameters of the first magnetic field heat treatment include a magnetic field intensity of 0.3 T, a temperature of 48 DEG C and a time of 45 minutes.

[0015] Further, the working condition parameters of the second magnetic field heat treatment include a magnetic field intensity of 0.8 T, a temperature of 63 DEG C and a time of 75 minutes.

[0016] Further, the low-temperature plasma treatment comprises the following processes:

[0017] The Bupleurum root slices are laid in a cavity of a low-temperature plasma treatment device, vacuum is drawn, argon is introduced when the vacuum degree of the cavity reaches 10 Pa or less, the low-temperature plasma treatment is performed at an argon flow of 10-20 sccm and a power of 65-75 W for 90-120 seconds to obtain the pre-processed Bupleurum root slices.

[0018] Further, the power is 70 W, and the time of the low-temperature plasma treatment is 105 seconds.

[0019] Further, the head diameter of the fresh and clean Bupleurum root is greater than or equal to 0.5 cm.

[0020] Further, the thickness of the pre-processed Bupleurum root slices is 3-4 mm.

[0021] In a second aspect, based on the same inventive concept, the application provides a Bupleurum root slice, which is prepared by the preparation method of any one of the Bupleurum root slices in the first aspect.

[0022] Further, the content of aflatoxin B1 in the Bupleurum root slice is less than or equal to 1.9 μg / kg after an accelerated test, wherein the working condition parameters of the accelerated test include a temperature of 40 DEG C, a relative humidity RH of 75%, and a time of 6 months.

[0023] Compared with the prior art, the above technical solution provided in the embodiments of the application has at least the following advantages:

[0024] The embodiment of the present application provides a bupleurum decoction piece and a preparation method thereof, compared with a traditional bupleurum decoction piece preparation process, the present application innovatively introduces low-temperature plasma technology and magnetic field heat treatment technology, which not only significantly improves the content of effective components such as saikosaponin in the obtained bupleurum decoction piece and the quality stability thereof, and effectively reduces the risk of producing aflatoxin during storage; and the present application omits the time-consuming dampening process in the traditional process, greatly improves the preparation efficiency, and provides a new process for the processing and production of bupleurum decoction piece. Specifically:

[0025] 1) On the one hand, the present application treats bupleurum root slices by low-temperature plasma, which can not only sterilize and preserve the bupleurum root slices quickly, but also enhance the permeability of the bupleurum root slices in a non-high-heat and high-humidity environment, which plays a similar softening role, so that the heat and magnetic field of subsequent magnetic field heat treatment can act more deeply on the internal organization of the bupleurum root slices, creating favorable conditions for subsequent magnetic field heat treatment;

[0026] 2) On the other hand, in the magnetic field heat treatment drying process, first, pre-drying is carried out under the conditions of weak magnetic field (0.1~0.4T) and low temperature (45~50℃), and the Lorentz force is used to act on water molecules and polar molecules (such as saikosaponin), so that the molecular dipole moment is arranged in a direction, which is beneficial to improve the drying effect under the conditions of subsequent medium-strength magnetic field (0.6~1.0T) and medium-high temperature (60~65℃); at the same time, the gradual drying method of two-stage gradient magnetic field heat treatment can effectively reduce the thermal shock of traditional high-temperature heat drying on fresh-cut bupleurum root slices, and further reduce the loss of effective components in the bupleurum root slices. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical scheme and advantages of the embodiments of the present application more clear, the technical scheme in the embodiments of the present application will be described clearly and completely below, obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of protection of the present application.

[0028] Unless otherwise specifically indicated, all raw materials, reagents, instruments and equipment used in the present application can be purchased on the market or prepared by existing methods. Meanwhile, the steps and parameters involved in the present application, if not specially limited or specifically described, can be carried out according to the existing technology disclosed in the preparation process of Radix Bupleuri decoction or directly using existing equipment, such as the magnetic field heat treatment process, which does not have strict special limitations or special technical requirements for equipment, as long as the drying is carried out under the preset magnetic field strength and temperature parameters. For example, existing magnetic field heat treatment equipment such as magnetic field heating furnace with equipment model GC800-15 or GC800-30, magnetic induction heat reactor with equipment model MTR-1, magnetic field assisted drying box with equipment model MFT10 / MFT50, or self-built suitable external magnetic field (for example, electromagnetic coil, permanent magnet array and other magnetic field generating modules can be installed inside or outside the drying machine) based on the existing heating medicinal material drying machine, etc. can be used to heat and dry medicinal materials in a magnetic field environment. The present application document will not be repeated here.

[0029] The present application will be further described in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present application and not to limit the scope of the present application. The experimental methods in the following examples are not specified, which are generally determined according to the national standards. If there is no corresponding national standard, the international standard, the conventional condition or the condition suggested by the manufacturer is used.

[0030] Example 1

[0031] This example provides a Radix Bupleuri decoction, and the preparation method of the Radix Bupleuri decoction comprises the following steps:

[0032] Step (1): Fresh Radix Bupleuri (specifically, North Radix Bupleuri, with root head diameter of 0.8±0.1 cm) is removed from mud and residual stems, washed and drained surface water, and then cut into Radix Bupleuri slices with thickness of 3.5 mm; the Radix Bupleuri slices are laid in the cavity of a low temperature plasma treatment device, vacuumized, and when the vacuum degree of the cavity reaches below 10 Pa, argon is introduced, and low temperature plasma treatment is carried out under the condition of argon flow of 15 sccm and power of 70 W for 105 seconds to obtain the pretreated Radix Bupleuri slices;

[0033] Step (2): The pretreated Radix Bupleuri slices obtained in step (1) are placed in a magnetic field heat treatment equipment (specifically, equipment model GC800-30), first treated under the condition of magnetic field strength of 0.3 T and temperature of 48℃ for 45 minutes, and then treated under the condition of magnetic field strength of 0.8 T and temperature of 63℃ for 75 minutes, and cooled to room temperature to obtain the Radix Bupleuri decoction.

[0034] Example 2

[0035] This example provides a radix bupleuri decoction piece, and the preparation method of the radix bupleuri decoction piece comprises the following steps:

[0036] Step (1): remove the mud and residual stems from fresh Bupleurum root (specifically, Bupleurum chinense, with a root head diameter of 0.8±0.1 cm), wash and drain the surface moisture, and then cut into Bupleurum root slices with a thickness of 3.0 mm; spread the Bupleurum root slices flat in the cavity of a low-temperature plasma treatment device, evacuate the cavity, and when the vacuum degree of the cavity reaches below 10 Pa, introduce argon gas, and perform low-temperature plasma treatment for 120 seconds under the conditions of an argon flow rate of 10 sccm and a power supply of 65 W to obtain the pretreated Bupleurum slices;

[0037] Step (2): placing the pretreated Bupleurum slices obtained in step (1) in a magnetic field heat treatment device (specific equipment model is GC800-30), first performing a first magnetic field heat treatment for 60 minutes under the conditions of a magnetic field intensity of 0.1T and a temperature of 45°C, then performing a second magnetic field heat treatment for 60 minutes under the conditions of a magnetic field intensity of 0.6T and a temperature of 65°C, and cooling to room temperature to obtain the Bupleurum slices.

[0038] Example 3

[0039] This example provides a radix bupleuri decoction piece, and the preparation method of the radix bupleuri decoction piece comprises the following steps:

[0040] Step (1): remove the mud and residual stems from fresh Bupleurum root (specifically, Bupleurum chinense, with a root head diameter of 0.8±0.1 cm), wash and drain the surface moisture, and then cut into Bupleurum root slices with a thickness of 4.0 mm; spread the Bupleurum root slices flat in the cavity of a low-temperature plasma treatment device, evacuate the cavity, and when the vacuum degree of the cavity reaches below 10 Pa, introduce argon gas, and perform low-temperature plasma treatment for 90 seconds under the conditions of an argon flow rate of 20 sccm and a power supply of 75 W to obtain the pretreated Bupleurum slices;

[0041] Step (2): placing the pretreated Bupleurum slices obtained in step (1) in a magnetic field heat treatment device (specific equipment model is GC800-30), first performing a first magnetic field heat treatment for 30 minutes under the conditions of a magnetic field intensity of 0.4T and a temperature of 50°C, then performing a second magnetic field heat treatment for 90 minutes under the conditions of a magnetic field intensity of 1.0T and a temperature of 60°C, and cooling to room temperature to obtain the Bupleurum slices.

[0042] Comparative Example 1

[0043] This example provides a method for preparing Bupleurum slices based on traditional fresh-cutting technology, comprising the following steps:

[0044] Fresh Bupleurum root (specifically, Bupleurum chinense, root head diameter of 0.8±0.1 cm) is removed from mud and residual stems, washed and drained of surface moisture, and then cut into Bupleurum root slices with a thickness of 3.5 mm; the Bupleurum root slices are placed in an oven for drying at a temperature of 63°C for 120 minutes, cooled to room temperature, and Bupleurum root slices are obtained.

[0045] Comparative Example 2

[0046] This example provides a method for preparing Bupleurum root slices using a traditional moistening process, comprising the following steps:

[0047] Fresh Bupleurum root (specifically, Bupleurum chinense, root head diameter of 0.8±0.1 cm) is removed from mud and residual stems, washed and drained of surface moisture, and then cut into Bupleurum root slices with a thickness of 3.5 mm; the Bupleurum root slices are placed in an oven for drying at a temperature of 63°C for 120 minutes, cooled to room temperature, and Bupleurum root slices are obtained.

[0048] Comparative Example 3

[0049] This example provides a Bupleurum root slice and a method for preparing the same, which differs from Example 1 only in that the low-temperature plasma technology is not used in step (1).

[0050] The method for preparing the Bupleurum root slice comprises the following steps:

[0051] Step (1): Fresh Bupleurum root (specifically, Bupleurum chinense, root head diameter of 0.8±0.1 cm) is removed from mud and residual stems, washed and drained of surface moisture, and then cut into Bupleurum root slices with a thickness of 3.5 mm;

[0052] Step (2): The Bupleurum root slices obtained in step (1) are placed in a magnetic field heat treatment device, first subjected to first magnetic field heat treatment at a magnetic field strength of 0.3T and a temperature of 48°C for 45 minutes, and then subjected to second magnetic field heat treatment at a magnetic field strength of 0.8T and a temperature of 63°C for 75 minutes, and cooled to room temperature to obtain the Bupleurum root slice.

[0053] Comparative Example 4

[0054] This example provides a Bupleurum root slice and a method for preparing the same, which differs from Example 1 only in that the Bupleurum root slices are not dried in a magnetic field environment in step (2).

[0055] The method for preparing the Bupleurum root slice comprises the following steps:

[0056] Step (1): Fresh Bupleurum root (specifically, Bupleurum chinense, root head diameter of 0.8±0.1 cm) is removed from mud and residual stems, washed and drained of surface moisture, and then cut into Bupleurum root slices with a thickness of 3.5 mm;

[0057] Step (2): placing the Bupleurum root slices obtained in step (1) in an oven, first performing a first drying treatment at a temperature of 48° C. for 45 minutes, then performing a second drying treatment at a temperature of 63° C. for 75 minutes, and cooling to room temperature to obtain the Bupleurum decoction slices.

[0058] Comparative Example 5

[0059] This example provides a radix bupleuri decoction piece and a preparation method thereof, which differs from Example 1 only in that a gradual drying method using a gradient magnetic field heat treatment is not used in step (2).

[0060] The preparation method of the Radix Bupleuri decoction pieces comprises the following steps:

[0061] Step (1): remove the mud and residual stems from fresh Bupleurum root (specifically, Bupleurum chinense, with a root head diameter of 0.8±0.1 cm), wash and drain the surface moisture, and then cut into Bupleurum root slices with a thickness of 3.5 mm; spread the Bupleurum root slices flat in the cavity of a low-temperature plasma treatment device, evacuate the cavity, and when the vacuum degree of the cavity reaches below 10 Pa, introduce argon gas, and perform low-temperature plasma treatment for 105 seconds under the conditions of an argon flow rate of 15 sccm and a power supply of 70 W to obtain the pretreated Bupleurum slices;

[0062] Step (2): placing the pretreated Bupleurum slices obtained in step (1) in a magnetic field heat treatment device, performing a second magnetic field heat treatment for 120 minutes at a magnetic field intensity of 0.8 T and a temperature of 63° C., and cooling to room temperature to obtain the Bupleurum slices.

[0063] Test Example 1

[0064] In this example, the content of saikosaponin a and saikosaponin d in the Bupleurum slices obtained in Examples 1 to 3 and Comparative Examples 1 to 5 were detected with reference to the high performance liquid chromatography method (General Rule 0512) recorded in the 2020 edition of the Chinese Pharmacopoeia. The total content of saikosaponin a and saikosaponin d was measured as shown in Table 1.

[0065] Table 1

[0066]

[0067] It can be seen from Table 1 that compared with Comparative Examples 1 to 5, the content of saikosaponin in the Bupleurum decoction pieces obtained in Examples 1 to 3 of the present invention is significantly improved, and the quality of the Bupleurum decoction pieces is higher.

[0068] Test Example 2

[0069] On the basis of test example 1, the Bupleurum decoction pieces obtained in examples 1-3 and comparative example 2 were further sealed and stored in polyethylene plastic packaging bags, and then subjected to an accelerated test at 40℃, RH 75% for 6 months. After the accelerated test, the content of aflatoxin B1 in each sample was detected by chromatography (chromatographic conditions including: the mobile phase was composed of methanol: acetonitrile: water with a volume ratio of 30:10:60, isocratic elution, the flow rate, column temperature and sample volume were 1.0 mL / min, 35℃, 10 μL respectively, and the fluorescence detector excitation wavelength and emission wavelength were 360 nm, 440 nm respectively), and the test results are shown in Table 2.

[0070] Table 2

[0071]

[0072] As can be seen from Table 2, compared with comparative example 2, the content of aflatoxin B1 in the Bupleurum decoction pieces obtained in examples 1-3 after the accelerated test is ≤1.9 μg / kg, which is much lower than the standard specified in the Chinese Pharmacopoeia (the limit of aflatoxin B1 is 5 μg / kg), and the Bupleurum decoction pieces can be stored for a long time. In addition, other index parameters such as the moisture content and the content of alcohol-soluble extract in the Bupleurum decoction pieces obtained in the examples all meet the requirements of the Pharmacopoeia.

[0073] In summary, the examples of the present application provide a Bupleurum decoction piece and a preparation method thereof. Compared with the traditional preparation process of Bupleurum decoction pieces, the present application innovatively introduces low-temperature plasma technology and magnetic field heat treatment technology, which not only significantly improves the content of effective components such as saikosaponin in the obtained Bupleurum decoction pieces and the quality stability thereof, and effectively reduces the risk of aflatoxin production during storage; and the present application omits the time-consuming dampening process in the traditional process, greatly improves the preparation efficiency, and provides a new process for the processing and production of Bupleurum decoction pieces.

[0074] Various embodiments of the present application can exist in the form of a range; it should be understood that the description in the form of a range is merely for the sake of convenience and brevity, and should not be understood as a rigid limitation on the scope of the present application; therefore, it should be considered that the described range has specifically disclosed all possible sub-ranges and single values within the range. For example, it should be considered that the range description from 1 to 6 has specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the range, such as 1, 2, 3, 4, 5 and 6, regardless of the range. In addition, whenever a numerical range is indicated in this document, it refers to any cited number (fraction or integer) within the indicated range.

[0075] The foregoing is considered as illustrative only of the principles of the application. Numerous modifications and changes will readily occur to those skilled in the art, and it is intended to embrace all such modifications and changes that fall within the scope of the application. Accordingly, the application is not to be restricted in scope to the specific embodiments disclosed herein but is to be accorded the full scope that the principles and novel features request appropriately granted.

Claims

1. A method for preparing Bupleurum slices, characterized in that: The preparation method of the Radix Bupleuri decoction pieces comprises the following steps: Fresh and clean bupleurum root was sliced ​​and then treated with low-temperature plasma to obtain pretreated bupleurum slices; Drying the pretreated bupleurum slices by a magnetic field heat treatment method to obtain the bupleurum slices; Wherein, the magnetic field heat treatment includes the following process: Performing a first magnetic field heat treatment for 30 to 60 minutes at a magnetic field intensity of 0.1 to 0.4 T and a temperature of 45 to 50° C.; Performing a second magnetic field heat treatment for 60 to 90 minutes at a magnetic field intensity of 0.6 to 1.0 T and a temperature of 60 to 65° C.; The low temperature plasma treatment includes the following processes: The radix bupleuri root slices were flattened in the cavity of a low-temperature plasma treatment device and evacuated. When the vacuum degree of the cavity reached below 10 Pa, argon gas was introduced and low-temperature plasma treatment was performed for 90 to 120 seconds at an argon flow rate of 10 to 20 sccm and a power supply of 65 to 75 W to obtain the pretreated radix bupleuri slices.

2. The method for preparing the Radix Bupleuri decoction pieces according to claim 1, wherein The working condition parameters of the first magnetic field heat treatment are: magnetic field intensity of 0.3T, temperature of 48°C, and time of 45 minutes.

3. The method for preparing the Radix Bupleuri decoction pieces according to claim 1, wherein The working condition parameters of the second magnetic field heat treatment are: magnetic field intensity of 0.8T, temperature of 63°C, and time of 75 minutes.

4. The method for preparing the Radix Bupleuri decoction pieces according to claim 3, wherein: The power of the power supply is 70W, and the time of the low-temperature plasma treatment is 105 seconds.

5. The method for preparing the Radix Bupleuri decoction pieces according to any one of claims 1 to 4, characterized in that: The root head diameter of the fresh and clean Bupleurum root is ≥0.5 cm.

6. The method for preparing the Radix Bupleuri decoction pieces according to any one of claims 1 to 4, characterized in that: The thickness of the pretreated Bupleurum slices is 3-4 mm.

7. A Bupleurum slice, characterized in that: The radix bupleuri decoction pieces are prepared by the preparation method of the radix bupleuri decoction pieces according to any one of claims 1 to 6.

8. The Bupleurum root slices according to claim 7, characterized in that The content of aflatoxin B1 in the Bupleurum slices after accelerated testing is ≤1.9 μg / kg; wherein, the working condition parameters of the accelerated test include: temperature of 40° C., relative humidity RH of 75%, and time of 6 months.

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