Traditional Chinese medicine composition, preparation method thereof and application of traditional Chinese medicine composition in preparation of product for improving immunity of pets

Abstract

The invention belongs to the field of veterinary drugs, and relates to a traditional Chinese medicine composition, a preparation method thereof and application of the traditional Chinese medicine composition in preparation of products for improving immunity of pets. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 30 to 60 parts of radix astragali seu hedysari, 20 to 50 parts of lucid ganoderma, 15 to 45 parts of fructus ziziphi jujubae, 15 to 45 parts of common fenugreek seed, 20 to 50 parts of phellinus igniarius, 20 to 50 parts of herba agrimoniae, 25 to 55 parts of fructus hippophae and 30 to 60 parts of fructus ligustri lucidi. The traditional Chinese medicine composition provided by the invention has relatively good safety and palatability, and is beneficial to improving the antibody level in a cat body and improving the micro-ecological health of an intestinal tract, so that the immunocompetence and the overall health condition of the cat are improved.

Description

Technical Field

[0001] The present invention belongs to the field of veterinary medicine, and relates to a traditional Chinese medicine composition, a preparation method thereof, and application thereof in preparing a product for improving the immunity of pets. Background Art

[0002] Natural plants that can serve as both medicine and food are widely used for disease prevention and health maintenance. Their unique therapeutic concepts and methods are gradually demonstrating their potential value in modern pet health management. In the field of pet nutrition, in particular, the development of functional feeds using natural plant extracts not only provides basic nutrition but also utilizes the plant's natural active ingredients to promote health and prevent disease.

[0003] Traditional Chinese Medicine (TCM)'s distinctive diagnostic and treatment models have demonstrated unique application value in the field of modern companion animal health. Based on the traditional Chinese medicine framework of "medicine and food share the same origin," functional feeds developed with active ingredients from herbal plants are gradually establishing a new pet health intervention system. These naturally derived functional preparations not only achieve a scientifically balanced diet, but more importantly, their medicinally active ingredients can synergistically enhance the body's immune homeostasis. This is manifested in multiple dimensions, including enhancing the efficacy of innate immune responses, regulating the adaptive immune system, and modulating intestinal mucosal barrier function. This nutritional intervention strategy, guided by TCM's holistic approach, achieves the principle of "preventive treatment" by balancing immune function at the molecular level. It not only meets the current needs of refined companion animal health management, but also aligns deeply with modern functional nutrition research at the mechanistic level. Technological exploration in this area not only expands the application boundaries of traditional medical theory but also provides scientific reference for the design of pet health management programs, helping to promote the standardized application of natural botanical preparations in veterinary clinical practice. It also provides pet owners with a useful guide to improving their pets' health through TCM principles and increases the acceptance of natural botanical extracts with the same origin as medicine and food in pet health management. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to address the deficiencies of the existing technology and provide a traditional Chinese medicine composition and a preparation method thereof and the application thereof in preparing a product for improving the immunity of pets, in particular, to provide a traditional Chinese medicine composition and a preparation method thereof and the application thereof in preparing a product for improving the immunity of cats.

[0005] In order to solve the above technical problems, the technical solutions adopted by the present invention are as follows: The present invention discloses a traditional Chinese medicine composition, which comprises the following components in parts by weight: 30-60 portions of Astragalus, 20-50 servings of Ganoderma lucidum, 15-45 servings of jujube, 15-45 parts fenugreek, 20-50 parts of Phellinus igniarius, 20-50 parts of Agrimony Root, 25-55 parts of sea buckthorn, 30-60 parts of Ligustrum lucidum fruit.

[0006] In some embodiments, preferably, it comprises the following components in parts by weight: 30-40 portions of Astragalus, 20-30 servings of Ganoderma lucidum, 15-25 servings of jujube, 15-25 parts fenugreek, 20-30 parts of Phellinus igniarius, 20-30 parts of Agrimony Root, 25-35 parts of sea buckthorn, 30-40 parts of Ligustrum lucidum fruit.

[0007] In some embodiments, further preferably, the traditional Chinese medicine composition comprises the following components in parts by weight: 30 parts of Astragalus, 20 servings of Ganoderma Lucidum, 15 servings of jujube, 15 servings of fenugreek, Phellinus igniarius 20 parts, 20 portions of Agrimony Root, 25 parts of sea buckthorn, 30 portions of Ligustrum lucidum fruit.

[0008] In some embodiments, more preferably, the Chinese medicine composition comprises the following components in parts by weight: 30 kg of Astragalus, 20 kg of Ganoderma lucidum, 15 kg of jujube, 15 kg of Fenugreek, 20 kg of Phellinus igniarius, 20 kg of Agrimoniae, 25 kg of Hippophae rhamnoides, and 30 kg of Ligustrum lucidum.

[0009] Furthermore, the present invention discloses a method for preparing the above-mentioned traditional Chinese medicine composition, which comprises mixing astragalus, ganoderma lucidum, jujube, fenugreek, mulberry linterus, agrimony, sea buckthorn, and ligustrum lucidum in the above-mentioned parts by weight, and extracting the mixture by a dynamic countercurrent reflux extraction method to obtain an extract of the traditional Chinese medicine composition.

[0010] In some embodiments, the extraction solvent used in the dynamic countercurrent reflux extraction method is water; and / or, the ratio of the mass of the extraction solvent used in the dynamic countercurrent reflux extraction method to the total mass of the raw material is (10~20):1; and / or, during extraction in the dynamic countercurrent reflux extraction method, the heating temperature is 70℃~90℃; and / or, during extraction in the dynamic countercurrent reflux extraction method, the extraction time is 2.0~3.0 hours.

[0011] In some embodiments, preferably, the extraction solvent used in the dynamic countercurrent reflux extraction method is water; and / or, the ratio of the mass of the extraction solvent used in the dynamic countercurrent reflux extraction method to the total mass of the raw material is 15:1; and / or, during extraction in the dynamic countercurrent reflux extraction method, the heating temperature is 80°C; and / or, during extraction in the dynamic countercurrent reflux extraction method, the extraction time is 2.5 hours.

[0012] The dynamic countercurrent reflux extraction method involves feeding the medicinal material in the opposite direction to the direction it enters the solvent. The extraction solvent's inlet direction is the direction of the slag discharge, while the outlet direction is the direction of the medicinal material. Therefore, the medicinal material is pushed through the extractor in the opposite direction to the flow of the liquid. As the medicinal material is pushed forward, its active ingredients dissolve. By the time it reaches the slag discharge port, the concentration of these active ingredients is very low. However, because the newly introduced water still has a certain concentration difference, a more complete extraction can be achieved.

[0013] In some embodiments, when the dosage form of the traditional Chinese medicine composition is an extract, the extract of the traditional Chinese medicine composition is further concentrated to obtain the extract; and / or, when the dosage form of the Chinese medicine composition is an extract, using a double-effect concentrator to concentrate the Chinese medicine composition extract to obtain the Chinese medicine composition extract; And / or, when the dosage form of the traditional Chinese medicine composition is an extract, when a double-effect concentrator is used to concentrate the extract of the traditional Chinese medicine composition to prepare the traditional Chinese medicine composition extract, the setting parameters of the double-effect concentrator are: the vacuum degree of the first effect is -0.06MPa to -0.04MPa, the evaporation temperature of the first effect is 80°C to 90°C, the vacuum degree of the second effect is -0.09MPa to -0.07MPa, and the evaporation temperature of the second effect is 60°C to 80°C; And / or, when the dosage form of the Chinese medicine composition is an extract, when a double-effect concentrator is used to concentrate the Chinese medicine composition extract to prepare the Chinese medicine composition extract, the double-effect concentrator is used to concentrate the Chinese medicine composition extract to a relative density of 1.15 to 1.25; and / or, When the dosage form of the Chinese medicine composition is a powder, the extract of the Chinese medicine composition is further concentrated to obtain an extract, and the extract is spray-dried to obtain the powder of the Chinese medicine composition.

[0014] In some embodiments, preferably, when the dosage form of the traditional Chinese medicine composition is an extract, when a double-effect concentrator is used to concentrate the traditional Chinese medicine composition extract to prepare the traditional Chinese medicine composition extract, the setting parameters of the double-effect concentrator are: the vacuum degree of the first effect is -0.05MPa, the evaporation temperature of the first effect is 85°C, the vacuum degree of the second effect is -0.08MPa, and the evaporation temperature of the second effect is 70°C.

[0015] In some embodiments, preferably, when the dosage form of the Chinese medicine composition is an extract, when a double-effect concentrator is used to concentrate the Chinese medicine composition extract to prepare the Chinese medicine composition extract, the double-effect concentrator is used to concentrate the Chinese medicine composition extract to a relative density of 1.20.

[0016] Furthermore, the present invention discloses a pharmaceutical preparation, which is prepared from the above-mentioned traditional Chinese medicine composition and one or more pharmaceutically acceptable excipients.

[0017] The dosage form of the pharmaceutical preparation is an extract, powder, granule, tablet, capsule, pill, pill, powder or liquid preparation; and / or the pharmaceutically acceptable excipient is any one or a combination of solubilizers, cosolvents, emulsifiers, colorants, adhesives, disintegrants, fillers, lubricants, wetting agents, osmotic pressure regulators, stabilizers, glidants, flavoring agents, preservatives, suspending agents, plasticizers, surfactants, thickeners, inclusion agents, humectants, absorbents, diluents, flocculants, deflocculants, filter aids and release retardants.

[0018] The use of the above-mentioned traditional Chinese medicine composition in the preparation of pet products is also within the scope of protection of the present invention.

[0019] Furthermore, the present invention discloses a pet product comprising the above-mentioned traditional Chinese medicine composition or the above-mentioned pharmaceutical preparation.

[0020] In some embodiments, the product is a veterinary drug, a pet feed additive, or a pet feed; and / or, the pet is a cat or a dog.

[0021] When the product is a pet feed additive, the extract of the traditional Chinese medicine composition or the pharmaceutical preparation is added to the pet feed; preferably, the amount of the extract of the traditional Chinese medicine composition added to the pet feed is 1-2 kg of the extract of the traditional Chinese medicine composition per ton of pet feed; preferably, the amount of the pharmaceutical preparation added to the pet feed is 1-2 kg of the pharmaceutical preparation per ton of pet feed.

[0022] The use of the above-mentioned traditional Chinese medicine composition, the above-mentioned pharmaceutical preparation or the above-mentioned pet product in the preparation of a product for improving the immunity of a pet is also within the scope of protection of the present invention.

[0023] Specifically, the product for improving pet immunity has the following functions: i. Improve the pet's cellular immunity; and / or, ii. Improve the specific immune function of pets; and / or, iii. Improve the nonspecific immune function of pets; and / or, iv. Improve the nonspecific immunity of the pet's intestinal tract; and / or v. Effectively regulate and optimize the intestinal flora structure of pets.

[0024] The traditional Chinese medicine composition disclosed by the invention comprises the following components: astragalus root, ganoderma lucidum, jujube, fenugreek, mulberry linterus, agrimony, sea buckthorn and privet fruit.

[0025] The pharmacological properties of the various Chinese medicinal ingredients in the above-mentioned Chinese medicine composition are as follows: Astragalus: Sweet, slightly warm, enters the spleen and lung meridians. It has the effects of replenishing qi and raising yang, benefiting the lungs and strengthening the exterior, and is used for symptoms such as spleen and stomach qi deficiency, lung qi deficiency, and qi and blood deficiency.

[0026] Ganoderma lucidum: sweet, neutral; enters the heart, lung, liver and kidney meridians, has the effects of replenishing qi and calming the mind, relieving cough and relieving asthma. Modern research shows that Ganoderma lucidum has the effects of enhancing immunity and regulating blood sugar.

[0027] Jujube: sweet and warm; enters the spleen and stomach meridians, has the effects of tonifying the middle and replenishing qi, nourishing blood and calming the mind, and is often used for spleen deficiency syndrome.

[0028] Fenugreek: bitter, warm; enters the kidney meridian, has the effects of warming the kidney and assisting yang, dispelling cold and relieving pain, and is mainly used for kidney yang deficiency syndrome.

[0029] Phellinus igniarius: Slightly bitter and cold. It enters the liver and kidney meridians. It has the effects of promoting blood circulation and stopping bleeding, resolving fluid retention and stopping diarrhea, and softening and dispersing lumps. Modern research suggests it may also have immune-regulating, anti-inflammatory, antioxidant, and anti-tumor properties.

[0030] Agrimony: bitter, flat, enters the heart and liver meridians, has the effects of astringency, hemostasis, detoxification and tonification, and is used for various bleeding diseases and fatigue.

[0031] Seabuckthorn: Sweet, sour, and warm; it enters the spleen, lung, stomach, and heart meridians. It strengthens the spleen and aids digestion, relieves cough and expectoration, and promotes blood circulation and dissipates blood stasis. It is used to improve indigestion and loss of appetite.

[0032] Ligustrum lucidum fruit: Sweet, bitter, and cooling; enters the liver and kidney meridians. It nourishes the liver and kidneys, brightens eyesight, and darkens hair. It is used to treat soreness of the waist and knees, dizziness, and premature graying of hair caused by liver and kidney yin deficiency. It also improves vision loss and premature graying of hair (e.g., premature graying of hair). Modern applications include antioxidant properties, anti-aging, and immune regulation.

[0033] The present invention is formulated according to the principle of monarch, minister, assistant and envoy in traditional Chinese medicine, and combined with modern pharmacological research on effective ingredients. In the Chinese medicine composition of the present invention, astragalus and ganoderma are used as monarch drugs, which can tonify the middle and replenish qi, strengthen the exterior qi, and tranquilize the mind. Fenugreek and jujube are used as ministerial drugs, which assist the monarch drugs in tonifying the middle and replenishing qi, nourishing blood and tranquilizing the mind, while warming the kidney and supporting yang, thereby improving the body's anti-stress ability. Phellinus igniarius and Agrimoniae scabra are used as adjuvant drugs, which can promote blood circulation and dissipate blood stasis, replenish blood and stop bleeding, prevent warming and tonic drugs from causing excessive heatiness and blood heat, and can also detoxify and replenish deficiency, improve fatigue syndrome, and assist in improving the cat's anti-stress ability. Hippophae rhamnoides and Ligustrum lucidum are used as envoy drugs, which can astringe and stop diarrhea, nourish the liver and kidneys, and improve digestive function and body functions.

[0034] Cats are susceptible to cold pathogens and have low immunity. They also have weak gastrointestinal function and are prone to diarrhea. The Chinese medicine formula of the present invention is mainly based on tonifying the middle and replenishing qi, supplemented by nourishing blood and calming the mind, and warming the kidney and supporting yang. It also detoxifies, replenishes deficiency, and nourishes the liver and kidneys. The combination of these medicines stimulates the body's cellular immunity and humoral immunity, while improving the cat's physique and enhancing its anti-stress effect.

[0035] The present invention is developed based on the unique physique of cats. The traditional Chinese medicine composition provided by the present invention is different from the general formula for tonifying the middle and replenishing qi to enhance immunity. The traditional Chinese medicine composition of the present invention is added with traditional Chinese medicines that warm the kidney and enhance yang, detoxify and replenish deficiency, strengthen the stomach and aid digestion, astringe and stop diarrhea, and nourish the liver and kidneys, which is more effective for the health of cats.

[0036] Beneficial effects: (1) The Chinese medicine composition provided by the present invention has the effects of replenishing both Qi and blood; it is safe, has no side effects, and is better at improving the immune level than ordinary Barf baked cat food.

[0037] (2) The Chinese medicine composition provided by the present invention can significantly increase the number of white blood cells, the proportion of neutrophils and the proportion of lymphocytes in the blood of cats within the normal reference range, and has an effect of improving the cellular immunity of cats.

[0038] (3) The Chinese medicine composition provided by the present invention can increase the levels of rabies-specific antibodies and feline panleukopenia-specific antibodies produced by cats after vaccination, and has a significant enhancing effect on the specific immune function of cats.

[0039] (4) The Chinese medicine composition provided by the present invention can respectively increase the level of fecal sIgA and serum IgG antibodies, thereby enhancing the nonspecific immune ability of cats in the whole body and local intestine.

[0040] (5) The experimental results show that the richness of the cat's intestinal flora is improved after intervention with the Chinese medicine composition provided by the present invention, which is beneficial to maintaining the homeostasis of the intestinal microecology.

[0041] (6) The Chinese medicine composition provided by the present invention has no adverse effects on the overall diversity of the bacterial community, and the bacterial community structure is relatively stable.

[0042] (7) After being treated with the Chinese medicine composition provided by the present invention, cats form a certain specific microbial community structure, which helps to maintain or enhance the specificity of intestinal flora and immune homeostasis.

[0043] (8) The Chinese medicine composition provided by the present invention can effectively regulate and optimize the intestinal flora structure after intervention.

[0044] (9) The Chinese medicine composition provided by the present invention has significant therapeutic effects and can improve the immune ability of cats.

[0045] (10) The Chinese medicine composition provided by the present invention is safe, has significant efficacy, low cost, good efficiency, good palatability, and is easy to use.

[0046] (11) The Chinese medicine composition provided by the present invention has good safety and palatability, which is beneficial to increasing the antibody level in cats and improving the intestinal microecological health, thereby improving the cat's immune ability and overall health. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, and the above and / or other advantages of the present invention will become more apparent.

[0048] Figure 1 This is a data chart showing changes in cat's cellular immune function.

[0049] Figure 2 This is a graph showing changes in cat's specific immune function.

[0050] Figure 3 This is a data chart showing changes in cats' nonspecific immune function.

[0051] Figure 4 A diagram showing changes in intestinal microbial diversity.

[0052] Figure 5 This is a health status diagram of cats in some experimental groups of the present invention. DETAILED DESCRIPTION

[0053] The present invention can be better understood according to the following examples. However, it is easy for those skilled in the art to understand that the contents described in the examples are only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims.

[0054] The experimental methods described in the following examples are conventional methods unless otherwise specified; the reagents and materials are commercially available unless otherwise specified.

[0055] Technical and scientific terms in the present invention shall be understood according to their definitions if they are defined, and shall be understood according to their general meanings in the art if they are not defined.

[0056] The dynamic countercurrent reflux extraction method utilized in the embodiments of the present invention is one in which the direction of medicinal material feed and the direction of solvent entry are opposite. In this method, water is used, with the liquid inlet direction corresponding to the direction of slag discharge and the liquid outlet direction corresponding to the direction of medicinal material feed. Therefore, the direction in which the medicinal material is advanced through the extractor is opposite to the direction of liquid flow. As the medicinal material is continuously advanced, the active ingredient dissolves, and by the time it approaches the slag outlet, the concentration of the active ingredient is very low. However, since the newly introduced water still has a certain concentration difference, a more complete extraction can be achieved.

[0057] The health status of cats in some experimental groups of the present invention is shown in the figure Figure 5 shown.

[0058] Example 1: Preparation of Chinese herbal compound

[0059] Formula: 30 kg of Astragalus, 20 kg of Ganoderma lucidum, 15 kg of jujube, 15 kg of Fenugreek, 20 kg of Phellinus igniarius, 20 kg of Agrimoniae, 25 kg of Seabuckthorn, and 30 kg of Ligustrum lucidum.

[0060] Preparation method: After screening and cleaning each Chinese medicine component according to the above weight, the medicinal materials are crushed to 20-30 mesh, and then all the medicinal materials are mixed, and then extracted by dynamic countercurrent reflux extraction method, the extraction solvent is drinking water, the total mass ratio of the solvent used to the raw material is 15:1, the heating temperature is 80°C, and the extraction time is 2.5 hours to obtain a Chinese medicine compound extract; the above-obtained Chinese medicine compound extract is concentrated to a relative density of 1.2, wherein the concentration is carried out using a double-effect concentrator, and the vacuum degree and evaporation temperature of each effect are specifically: the vacuum degree of the first effect is -0.05MPa, and the evaporation temperature is 85°C; the vacuum degree of the second effect is -0.08MPa, and the evaporation temperature is 70°C; until the Chinese medicine compound extract is concentrated to a relative density of 1.20, the obtained extract is spray-dried to obtain the Chinese medicine compound extract powder.

[0061] Example 2: Animal Experiment

[0062] 1. Add Barf baked food with the product formula of the present invention The Chinese herbal compound extract powder prepared in Example 1 was added to the Maifudi Barf baked grain, with the added dosage being 1 kg of the Chinese herbal compound extract powder per ton of grain.

[0063] 2. Experimental Grouping Forty healthy, 12-month-old Chinese rural cats (half male and half female) with consistent levels of rabies antibodies and feline panleukopenia virus antibodies were selected. The cats were randomly divided into four groups: a blank control group (fed with a standard Mafia Barf baked food), an Immunity Test group (fed with a Mafia Barf baked food supplemented with the product of the present invention at a dose of 1 kg of a Chinese herbal compound extract powder per ton of food), a competitor control group A (fed with a complete weaning cat food called Guanneng), and a competitor control group B (fed with a freeze-dried, two-component Suligao immune granular cat food). Each group consisted of 10 cats (half male and half female) and fed the corresponding cat food for 42 consecutive days.

[0064] 3. Feeding and management All cats were housed in individual cages, with separate drinking water, food, and litter boxes. They were placed in two separate housing rooms to facilitate observation and recording. The cats were housed in a clean environment (referring to GB 14925-2010 Standard for Laboratory Animal Environments and Facilities), exposed to natural light, and interacted with their own cats and the experimenters at least once daily. They were provided with ample clean drinking water, which was changed twice daily. They were fed once daily in the afternoon, with free access to food. The litter boxes were cleaned twice daily, and proper ventilation was maintained in the housing room.

[0065] 4. General records On day 0 of the experiment, the test cats were immunized with rabies vaccine and feline triple vaccine. Subsequently, the mental state, food intake, fecal index and abnormal conditions of the test animals were recorded daily, and the experimental cats were weighed once a week.

[0066] 5. Changes in cats’ cellular immune function On the 42nd day of the experiment, anticoagulated blood was collected from the test cats, and the number of white blood cells, the proportion of neutrophils and the proportion of lymphocytes in the blood were measured using a hematology counter to evaluate the level of cellular immunity of the test cats.

[0067] The experimental results are as follows Figure 1 As shown, Figure A is the data of white blood cell count, Figure B is the data of neutrophil ratio, and Figure C is the data of lymphocyte ratio. The gray background area indicates the normal reference range, and * indicates the difference compared with the blank control group. P <0.05, ** indicates comparison with blank control group P <0.01. As shown in the figure, in the Immune Treasure test group, the white blood cell count, neutrophil ratio, and lymphocyte ratio were significantly higher than those in the blank control group (**P<0.01), and these elevated values ​​remained within the normal reference range. Competitor Control Groups A and B also significantly increased neutrophil and lymphocyte ratios compared to the blank control group (*P<0.05, **P<0.01).

[0068] The above results indicate that the Chinese medicine composition provided by the present invention can significantly increase the number of white blood cells, the proportion of neutrophils and the proportion of lymphocytes in the blood of cats within the normal reference range, and has an effect of improving the cellular immunity of cats.

[0069] 5. Changes in cats’ specific immune function On day 0 of the experiment, all test cats were injected with rabies vaccine and feline triple vaccine. Blood was collected from the test cats on days 0, 14, 28 and 42 of the experiment, and the serum was separated. The levels of feline rabies-specific antibodies and feline panleukopenia-specific antibodies were detected using ELISA to evaluate the ability of the Immune Treasure formula to enhance the level of cat-specific antibodies.

[0070] The results of changes in cat's specific immune function are as follows Figure 2 As shown, Figure A is the data of rabies specific antibody level, and Figure B is the data of feline panleukopenia specific antibody level, where * indicates that the difference between the control group and the blank group is significant. P <0.05, ** indicates comparison with blank control group P <0.01.

[0071] It can be seen from the figure that: (1) On the 0th day of the experiment, the rabies-specific antibodies and feline panleukopenia-specific antibodies in each group were at a low level. After the injection of the vaccine, on the 14th, 28th and 42nd days, the rabies-specific antibodies and feline panleukopenia-specific antibodies in the Immune Treasure test group were significantly improved compared with the blank control group (*P<0.05, **P<0.01).

[0072] (2) On the 42nd day of the experiment, the rabies-specific antibody level of the competitor control group A was significantly higher than that of the blank control group (**P<0.01), and exceeded that of the immune treasure test group; however, on the 14th and 28th days, there was no significant difference in the rabies-specific antibody level of the competitor control group A compared with the blank control group.

[0073] (3) On the 14th, 28th and 42nd days of the experiment, there was no significant difference in the rabies-specific antibody levels in the competitor control group B compared with the blank control group.

[0074] (4) For feline panleukopenia-specific antibodies, both competitor control group B and competitor control group A had a certain improvement effect, but both were lower than the immune treasure test group, and there was no significant difference with the blank control group.

[0075] The above test results show that the Chinese medicine composition provided by the present invention can increase the levels of rabies-specific antibodies and feline panleukopenia-specific antibodies produced by cats after vaccination, and has a significant enhancing effect on the specific immune function of cats.

[0076] 6. Changes in cats’ nonspecific immune function On days 14, 28, and 42 of the study, blood was collected from the test cats, and serum was separated. IgG antibody levels in the serum were measured using ELISA. On day 42, feces were collected from the test cats, and a 10% fecal homogenate was prepared. The supernatant was collected and fecal sIgA levels were measured using ELISA. IgG and sIgA are important nonspecific antibodies in cats and are used to assess the nonspecific immune capacity of cats' systemic and intestinal mucosal immunity, respectively.

[0077] Data on changes in cats' nonspecific immune function Figure 3 As shown, Figure A is the data of sIgA level in feces, and Figure B is the data of IgG antibody level in serum, where * indicates that the difference between the control group and the control group is significant.P <0.05, ** indicates comparison with blank control group P <0.01.

[0078] As can be seen from the figure: (1) Compared with the blank control group, the sIgA levels in the feces of the ImmunoTreasure test group, the competitor control group A and the competitor control group B were significantly increased (**P<0.01).

[0079] (2) Compared with the blank control group, the serum IgG antibody level in the Immune Treasure experimental group was significantly increased on the 28th and 42nd days of the experiment (*P<0.05).

[0080] (3) Compared with the blank control group, there was no significant difference in the serum IgG antibody levels of the competitor control group A and the competitor control group B on the 14th day, the 28th day, and the 42nd day.

[0081] The above test results show that the Chinese medicine composition provided by the present invention can respectively increase the level of fecal sIgA and serum IgG antibodies, thereby enhancing the nonspecific immunity of cats systemically and locally in the intestine.

[0082] 7. Changes in intestinal microbial diversity in cats On the 42nd day of the experiment, sterile swabs were used to collect rectal content samples from the test cats, and genomic DNA was extracted and amplified. The amplified products were sequenced and then subjected to bioinformatics analysis to evaluate changes in intestinal microorganisms.

[0083] The Chao1 index and ACE index are primarily used to estimate the species richness of microbial communities, reflecting the number of rare species that may exist but have not been detected. The Shannon index comprehensively considers species richness and evenness; higher values ​​indicate a more diverse community and a more even distribution of species. The Simpson index focuses on the dominance of dominant species; higher values ​​indicate a less diverse community dominated by a few dominant species. These four indices are used to assess the impact of community structure, ecological stability, and external disturbances on microbial diversity in the measurement of microbial diversity changes.

[0084] Figure 4 Figure 1 is a graph showing changes in intestinal microbial diversity. Figure A shows the ACE index data, Figure B shows the Chao index data, Figure C shows the Shannon index data, Figure D shows the Simpson index data, E shows the species distribution Venn diagram, and F shows the PCA analysis diagram. Contol represents the blank control group, MYB represents the immune treasure test group, CP_A represents the competitor control group A, and CP_B represents the competitor control group B. Unless otherwise specified, * indicates comparison with the blank control group. P <0.05, ** indicates comparison with blank control group P<0.01, *** indicates comparison with blank control group P <0.001.

[0085] As can be seen from the figure: (1) The results of the ACE index (Figure A) and the Chao index (Figure B) show that the richness of the intestinal flora in the Immunity Treasure test group was significantly higher than that in the blank control group (Control) (**P<0.01), indicating that the richness of the cat's intestinal flora may have been improved after the intervention of the Chinese herbal composition, which is beneficial to maintaining the homeostasis of the intestinal microecology. The competitor control group A (CP_A) showed a higher richness, while the competitor control group B (CP_B) showed a richness close to that of the blank control group.

[0086] (2) In the Shannon index (Figure C) and Simpson index (Figure D), the differences between the groups did not reach a statistically significant level, indicating that the Chinese medicine composition provided by the present invention had no adverse effects on the overall diversity of the microbial community and the microbial community structure was relatively stable.

[0087] (3) As shown in Figure E, the four groups had a total of 256 shared OTUs, accounting for 41.09% of the total. The Immune Treasure experimental group had 33 unique OTUs (accounting for 5.30% of the total OTUs), which was significantly higher than the competitor control group A (25, 4.01%) and the blank control group (27, 4.33%). This indicates that the Immune Treasure experimental group formed a certain specific microbial community structure after treatment, which helps to maintain or improve the specificity of the intestinal flora and immune homeostasis.

[0088] (4) As shown in Figure F, the PCA analysis results show that (where * indicates the comparison between the two groups P <0.05, ** indicates the difference between the two groups P <0.01, *** indicates the comparison between the two groups P <0.001), there were certain differences in the intestinal flora structure of each group (PC1 and PC2 together explained about 20% of the flora variation), and the Immune Treasure test group showed more obvious clustering compared with the other groups. In the direction of the PC2 axis, the Immune Treasure test group was clearly different from the other groups, and the difference reached a significant level (there were statistical differences between the Immune Treasure test group and the Control group and the competitor group, **P<0.01 or ***P<0.001), further suggesting that the Chinese medicine composition provided by the present invention can effectively regulate and optimize the intestinal flora structure.

[0089] Results from implementation cases of the present invention demonstrate that the addition of the product of the present invention can enhance the immune capacity of pet cats, including innate immunity, specific immunity, and intestinal immunity, with significantly better results than some commercially available products. The product provided by the present invention is suitable for enhancing the disease resistance of sensitive individuals and can effectively reduce the risk of infection during seasonal transitions, vaccination periods, and in mixed-pet environments. Furthermore, its optimized palatability and burden-free metabolic properties make it safe for pets to consume long-term, providing a convenient and efficient preventative health management solution for families with pets.

[0090] The present invention provides a traditional Chinese medicine composition, a method for preparing the composition, and a method for using the composition in preparing a product for enhancing pet immunity. Numerous methods and approaches exist for implementing this technical solution. The foregoing description is merely a preferred embodiment of the present invention. It should be noted that those skilled in the art may make various improvements and modifications without departing from the principles of the present invention, and such improvements and modifications are also within the scope of protection of the present invention. Any components not specified in this embodiment may be implemented using existing technologies.

Claims

1. A Chinese medicine composition, characterized in that It contains the following components in parts by weight: 30-60 portions of Astragalus, 20-50 servings of Ganoderma lucidum, 15-45 servings of jujube, 15-45 parts fenugreek, 20-50 parts of Phellinus igniarius, 20-50 parts of Agrimony Root, 25-55 parts of sea buckthorn, 30-60 parts of Ligustrum lucidum fruits.

2. The Chinese medicine composition according to claim 1, characterized in that It contains the following components in parts by weight: 30-40 portions of Astragalus, 20-30 servings of Ganoderma lucidum, 15-25 servings of jujube, 15-25 parts fenugreek, 20-30 parts of Phellinus igniarius, 20-30 parts of Agrimony Root, 25-35 parts of sea buckthorn, 30-40 parts of Ligustrum lucidum fruit; Preferably, the Chinese medicine composition comprises the following components in parts by weight: 30 parts of Astragalus, 20 servings of Ganoderma Lucidum, 15 servings of jujube, 15 servings of fenugreek, Phellinus igniarius 20 parts, 20 portions of Agrimony Root, 25 parts of sea buckthorn, 30 parts of Ligustrum lucidum fruit; Further preferably, the Chinese medicine composition comprises the following components in parts by weight: 30 kg of Astragalus, 20 kg of Ganoderma lucidum, 15 kg of jujube, 15 kg of Fenugreek, 20 kg of Phellinus igniarius, 20 kg of Agrimoniae, 25 kg of Hippophae rhamnoides, and 30 kg of Ligustrum lucidum.

3. The method for preparing the Chinese medicine composition according to any one of claims 1 to 2, characterized in that: The astragalus root, ganoderma lucidum, jujube, fenugreek, mulberry lindera, agrimony, sea buckthorn and glossy privet fruit in the weight proportions as claimed in any one of claims 1 to 2 are mixed and extracted by a dynamic countercurrent reflux extraction method to obtain a traditional Chinese medicine composition extract.

4. The preparation method according to claim 3, characterized in that The extraction solvent used in the dynamic countercurrent reflux extraction method is water; and / or, the ratio of the mass of the extraction solvent used in the dynamic countercurrent reflux extraction method to the total mass of the raw material drug is (10~20):1; and / or, during extraction in the dynamic countercurrent reflux extraction method, the heating temperature is 70°C~90°C; and / or, during extraction in the dynamic countercurrent reflux extraction method, the extraction time is 2.0~3.0 hours.

5. The preparation method according to claim 3, characterized in that When the dosage form of the Chinese medicine composition is an extract, the extract of the Chinese medicine composition is further concentrated to obtain the extract; and / or, when the dosage form of the Chinese medicine composition is an extract, using a double-effect concentrator to concentrate the Chinese medicine composition extract to obtain the Chinese medicine composition extract; And / or, when the dosage form of the traditional Chinese medicine composition is an extract, when a double-effect concentrator is used to concentrate the extract of the traditional Chinese medicine composition to prepare the traditional Chinese medicine composition extract, the setting parameters of the double-effect concentrator are: the vacuum degree of the first effect is -0.06MPa to -0.04MPa, the evaporation temperature of the first effect is 80°C to 90°C, the vacuum degree of the second effect is -0.09MPa to -0.07MPa, and the evaporation temperature of the second effect is 60°C to 80°C; And / or, when the dosage form of the Chinese medicine composition is an extract, when a double-effect concentrator is used to concentrate the Chinese medicine composition extract to prepare the Chinese medicine composition extract, the double-effect concentrator is used to concentrate the Chinese medicine composition extract to a relative density of 1.15 to 1.25; and / or, When the dosage form of the Chinese medicine composition is a powder, the extract of the Chinese medicine composition is further concentrated to obtain an extract, and the extract is spray-dried to obtain the powder of the Chinese medicine composition.

6. A pharmaceutical preparation, characterized in that The pharmaceutical preparation is prepared from the traditional Chinese medicine composition according to any one of claims 1 to 2 and one or more pharmaceutically acceptable excipients.

7. Use of the traditional Chinese medicine composition according to any one of claims 1 to 2 in the preparation of pet products.

8. A pet product, characterized in that: The invention comprises the Chinese medicine composition according to any one of claims 1 to 2 or the pharmaceutical preparation according to claim 6.

9. The pet product according to claim 8, characterized in that The product is a veterinary drug, a pet feed additive or a pet feed; and / or the pet is a cat or a dog.

10. Use of the traditional Chinese medicine composition according to any one of claims 1 to 2, the pharmaceutical preparation according to claim 6, or the pet product according to claim 8 in the preparation of a product for improving the immunity of pets.

11. The use according to claim 10, characterized in that The product for improving pet immunity has the following multiple functions: i. Improve the pet's cellular immunity; and / or, ii. Improve the specific immune function of pets; and / or, iii. Improve the nonspecific immune function of pets; and / or, iv. Improve the nonspecific immunity of the pet's intestinal tract; and / or v. Effectively regulate and optimize the intestinal flora structure of pets.

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