Skin recuperating method for regulating aquaporin based on multi-waveform intermediate-frequency electric signals

Abstract

The invention discloses a skin recuperating method for regulating aquaporin based on multi-waveform medium-frequency electric signals, and relates to the technical field of skin physical therapy, which comprises the following steps: (a) symmetrically attaching a pair of annular medical zinc alloy electrodes to a skin treatment area as an anode and a cathode respectively; (b) starting a preset treatment program through a program control module, and outputting intermediate-frequency current with the frequency range of 1kHz-10kHz to the electrode; (c) dynamically switching or superposing the intermediate-frequency current to output at least two waveforms of sine waves, sharp waves and square waves; (d) up-regulating the expression of aquaporin AQP3 in keratinocytes by utilizing the synergistic effect of zinc ions released by the zinc alloy electrode and multi-waveform electrical stimulation; ions continuously released by the zinc electrode permeate into an epidermal substrate layer, metal response element binding factors are activated, and the transcriptional activity of skin moisturizing and barrier repairing genes is enhanced.

Description

Technical Field

[0001] The present invention relates to the technical field of skin physical therapy, in particular to a skin recuperation method based on regulating aquaporins with multi-waveform intermediate frequency electrical signals. Background Art

[0002] In the field of skin physical therapy, the repair of dry and sensitive skin, hormone-dependent dermatitis, and chronic wounds such as diabetic foot ulcers has long faced core challenges:

[0003] Lack of aquaporin regulation: Clinical studies have confirmed that skin barrier dysfunction is directly related to downregulation of AQP3 aquaporin expression in keratinocytes, leading to increased transepidermal water loss and delayed repair. However, existing technologies lack non-invasive targeted regulation of AQP3.

[0004] Limitations of electrical stimulation technology: Traditional electrical stimulation devices often use fixed-frequency waveforms (such as a single sine wave), which can easily induce cellular electrical adaptability and cause stimulation efficiency to decay over time. Electrode materials commonly used are stainless steel or silver / silver chloride, which present issues such as insufficient biocompatibility (risk of sensitization), poor conductive stability (electrolyte dependence), and a lack of therapeutic synergy.

[0005] Low wound repair efficiency: Chronic wounds such as diabetic foot ulcers face the dual challenges of microcirculation disorders and bacterial biofilm resistance. Single drug treatment or physical therapy is unlikely to achieve deep tissue regeneration.

[0006] To address the above issues, a skin treatment method was designed that integrates precise frequency control, multiple waveform outputs, and a double-ring medical zinc alloy electrode structure. It is specifically used for non-invasive regulation of aquaporin expression to increase skin moisture content, improve dryness and sensitivity, and repair the skin barrier. Summary of the Invention

[0007] In view of the shortcomings of the existing technology, the present invention provides a skin care method based on regulating aquaporins by multi-waveform medium-frequency electrical signals, which solves the technical problems raised in the above-mentioned background technology.

[0008] To achieve the above objectives, the present invention is implemented through the following technical solutions: a skin care method based on regulating aquaporins with multi-waveform intermediate frequency electrical signals, comprising the following steps:

[0009] (a) A pair of annular medical zinc alloy electrodes are symmetrically attached to the skin treatment area, serving as the anode and cathode, respectively.

[0010] (b) Activating a preset treatment program through the program control module to output a medium-frequency current in the frequency range of 1 kHz–10 kHz to the electrodes;

[0011] (c) the intermediate frequency current dynamically switches or superimposes to output at least two waveforms of a sine wave, a spike wave, and a square wave;

[0012] (d) Zinc ions released from zinc alloy electrodes synergistically act with multi-waveform electrical stimulation to upregulate the expression of the water channel protein AQP3 in keratinocytes;

[0013] (e) Continue stimulation for 5–30 minutes to increase skin moisture and repair barrier function.

[0014] Preferably, the waveform switching in step (c) follows a cyclic timing sequence, including:

[0015] Output 3kHz sine wave for 1 minute;

[0016] Switch to 6kHz spike wave for 1 minute;

[0017] Switch to 10kHz square wave for 1 minute;

[0018] Repeat the cycle until treatment is complete.

[0019] Preferably, the annular medical zinc alloy electrode is fixed by a medical adhesive dressing, and a gel or solution containing electrolytes or barrier repair components is coated on the contact interface between the electrode and the skin.

[0020] Preferably, the skin impedance is monitored in real time during treatment, and the output current is automatically adjusted to adapt to the impedance change.

[0021] Preferably, the method is applied to the clinical treatment of dry and sensitive skin, hormone-dependent dermatitis, atopic dermatitis or diabetic foot ulcers.

[0022] Preferably, including:

[0023] a frequency control module configured to generate a 1kHz–10kHz intermediate frequency current;

[0024] Waveform output module, supports alternating or superimposed output of sine wave, spike wave and square wave;

[0025] A pair of annular medical zinc alloy electrodes electrically connected to the waveform output module;

[0026] The program control module presets the waveform cycle timing as described in claim 2.

[0027] Preferably, the annular medical zinc alloy electrode is a replaceable structure, and the surface of the electrode is covered with a biocompatible conductive coating.

[0028] Preferably, it is characterized in that an impedance feedback unit is provided to dynamically adjust the current output power according to skin resistance.

[0029] Beneficial effects

[0030] The present invention provides a skin treatment method based on regulating aquaporins with multi-waveform intermediate-frequency electrical signals. The present invention achieves a triple breakthrough mechanism by synergizing dynamically circulating multi-waveform intermediate-frequency electrical signals (1-10kHz sine wave, spike wave, and square wave timing alternation) with the biological activity of a ring-shaped zinc electrode:

[0031] Electrophysiological-biochemical dual pathway regulation:

[0032] Medium-frequency current activates calcium channels in keratinocytes, triggering Ca 2+ / Calmodulin signaling cascade, directly upregulating the transcriptional expression of the water channel protein AQP3;

[0033] The ions continuously released by the zinc electrode penetrate into the basal layer of the epidermis, activate the metal response element binding factor (MTF-1), and enhance the transcriptional activity of skin moisturizing and barrier repair genes;

[0034] Waveform Timing Overcomes Cell Adaptability Issues:

[0035] Sine wave gently opens ion channels → sharp wave strengthens cell membrane depolarization → square wave maintains transmembrane potential homeostasis. The three waveform cycles break the cell's electrical adaptation inertia and continuously stimulate AQP3 synthesis.

[0036] Non-invasive precision repair:

[0037] The electric field of the ring electrode is evenly distributed in the epidermis (50-100 μm depth), avoiding local burns;

[0038] Zinc ions simultaneously promote the reorganization of the lipid bilayer and the secretion of antimicrobial peptides, achieving an integrated repair of "increased water content → reconstruction of the lipid barrier → balance of microecology", significantly improving the redness and desquamation of dry and sensitive skin, accelerating the healing of wounds such as diabetic foot, and reducing recurrence. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 This is a schematic flow chart of the skin care method based on regulating aquaporins with multi-waveform intermediate frequency electrical signals according to the present invention.

[0040] Figure 2 This is a schematic diagram of the mechanism of action of Example 3 of the skin care method based on regulating aquaporins with multi-waveform medium-frequency electrical signals according to the present invention. DETAILED DESCRIPTION

[0041] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0042] See also Figure 1-2 The present invention provides a technical solution: a skin care method based on regulating aquaporin by multi-waveform intermediate frequency electrical signals, comprising the following steps:

[0043] (a) A pair of annular medical zinc alloy electrodes are symmetrically attached to the skin treatment area, serving as the anode and cathode, respectively.

[0044] (b) Activating a preset treatment program through the program control module to output a medium-frequency current in the frequency range of 1 kHz–10 kHz to the electrodes;

[0045] (c) the intermediate frequency current dynamically switches or superimposes to output at least two waveforms of a sine wave, a spike wave, and a square wave;

[0046] (d) Zinc ions released from zinc alloy electrodes synergistically act with multi-waveform electrical stimulation to upregulate the expression of the water channel protein AQP3 in keratinocytes;

[0047] (e) Continue stimulation for 5–30 minutes to increase skin moisture and repair barrier function.

[0048] This embodiment is further configured such that the waveform switching in step (c) follows a cyclic timing sequence, including:

[0049] Output 3kHz sine wave for 1 minute;

[0050] Switch to 6kHz spike wave for 1 minute;

[0051] Switch to 10kHz square wave for 1 minute;

[0052] Repeat the cycle until treatment is complete.

[0053] This embodiment is further configured such that the annular medical zinc alloy electrode is fixed by a medical adhesive dressing, and a gel or solution containing electrolytes or barrier repair components is coated on the contact interface between the electrode and the skin.

[0054] This embodiment is further configured to monitor skin impedance in real time during treatment and automatically adjust the output current to adapt to impedance changes.

[0055] This embodiment further provides that the method is applied to the clinical treatment of dry and sensitive skin, hormone-dependent dermatitis, atopic dermatitis or diabetic foot ulcers.

[0056] This embodiment is further configured to include:

[0057] a frequency control module configured to generate a 1kHz–10kHz intermediate frequency current;

[0058] Waveform output module, supports alternating or superimposed output of sine wave, spike wave and square wave;

[0059] A pair of annular medical zinc alloy electrodes electrically connected to the waveform output module;

[0060] The program control module presets the waveform cycle timing as described in claim 2.

[0061] This embodiment is further configured such that the annular medical zinc alloy electrode is a replaceable structure, and the surface of the electrode is covered with a biocompatible conductive coating.

[0062] This embodiment is further configured and characterized by providing an impedance feedback unit for dynamically adjusting the circuit output power according to skin resistance.

[0063] The detailed connection means are well-known technologies in this field. The following mainly introduces the working principle and process. The specific operations are as follows.

[0064] Example:

[0065] Example 1: Device implementation details

[0066] 1. Ring zinc electrode design

[0067] Material: Medical zinc alloy (Zn ≥ 99.9%), double ring structure (outer diameter 30mm / inner diameter 20mm)

[0068] Surface treatment: Laser microporous (aperture 50μm) to enhance zinc ion release

[0069] Ion release: Constant release of Zn under electrical stimulation 2+ (0.8μg / cm 2 / min)

[0070] 2. Electrical signal generation system

[0071] Frequency control: 1–10kHz adjustable, accuracy ±0.1Hz

[0072] Waveform switching logic (MCU preset program):

[0073] 3kHz sine wave → lasts for 1 minute → 6kHz spike wave → lasts for 1 minute → 10kHz square wave → lasts for 1 minute → loop

[0074] Impedance Adaptation: Real-time current adjustment (1–3 mA) to maintain a constant electric field.

[0075] Example 2: Standard treatment process

[0076] Step 1: Treatment Preparation

[0077] Cleanse your skin and apply zinc hyaluronic acid gel

[0078] Fix the ring electrodes on the treatment area (such as both sides of the cheeks)

[0079] Step 2: Execute the program

[0080] To start a preset cycle:

[0081] 3kHz sine wave (1 min) → 6kHz spike wave (1 min) → 10kHz square wave (1 min)

[0082] Total duration: 15 minutes (5 loops)

[0083] Step 3: Collaborative Repair

[0084] Apply ZnO nano repair patch externally after treatment.

[0085] Example 3: Dry and sensitive skin treatment verification

[0086] Key Effect

[0087] Stratum corneum water content: increased to 48.7AU after 4 weeks of treatment (32.1AU before treatment)

[0088] AQP3 expression: Immunohistochemistry showed that the protein expression intensity increased by 2.9 times

[0089] Barrier function: Transepidermal water loss (TEWL) decreased to 16.3g / m 2 / h(before treatment 28.9g / m 2 / h)

[0090] Mechanism of action: Figure 2 .

[0091] Example 4: Diabetic Foot Ulcer Adaptation Solution

[0092] Operational Adjustments

[0093] Waveform sequence: 5kHz sine wave (2 minutes) → 8kHz square wave (1 minute) → 10 seconds pause

[0094] Electrode placement: 1 cm outside the ulcer edge, 10 cm apart

[0095] Combined formulation: 1.2% sulfadiazine zinc added to gel

[0096] Efficacy observation

[0097] Wound healing time: 21.5 days (traditional method>30 days)

[0098] Bacterial load decreased by >99% (Staphylococcus aureus culture test)

[0099] It should be noted that, in this document, relational terms such as first and second, etc. are merely used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations.

Claims

1. A skin care method based on regulating aquaporins with multi-waveform intermediate frequency electrical signals, characterized in that: The following steps are involved: (a) A pair of annular medical zinc alloy electrodes are symmetrically attached to the skin treatment area, serving as the anode and cathode, respectively. (b) Activating a preset treatment program through the program control module to output a medium-frequency current in the frequency range of 1 kHz–10 kHz to the electrodes; (c) the intermediate frequency current dynamically switches or superimposes to output at least two waveforms of a sine wave, a spike wave, and a square wave; (d) Zinc ions released from zinc alloy electrodes synergistically act with multi-waveform electrical stimulation to upregulate the expression of the water channel protein AQP3 in keratinocytes; (e) Continue stimulation for 5–30 minutes to increase skin moisture and repair barrier function.

2. The skin care method based on multi-waveform intermediate frequency electrical signal regulation of aquaporins according to claim 1, characterized in that ,The waveform switching in step (c) follows a cyclic timing, including: Output 3kHz sine wave for 1 minute; Switch to 6kHz spike wave for 1 minute; Switch to 10kHz square wave for 1 minute; Repeat the cycle until treatment is complete.

3. The skin care method based on multi-waveform intermediate frequency electrical signal regulation of aquaporins according to claim 1, characterized in that The annular medical zinc alloy electrode is fixed by a medical adhesive dressing, and a gel or solution containing electrolytes or barrier repair components is coated on the contact interface between the electrode and the skin.

4. The skin care method based on multi-waveform intermediate frequency electrical signal regulation of aquaporins according to claim 1, characterized in that ,During the treatment process, the skin impedance is monitored in real time, and the output ,current is automatically adjusted to adapt to the impedance changes.

5. The skin care method based on regulating aquaporins by multi-waveform intermediate frequency electrical signals according to claim 1, characterized in that The method is applied to the clinical treatment of dry and sensitive skin, hormone-dependent dermatitis, atopic dermatitis or diabetic foot ulcers.

6. A device for implementing the method according to any one of claims 1 to 5, characterized in that: include: a frequency control module configured to generate a 1kHz–10kHz intermediate frequency current; Waveform output module, supports alternating or superimposed output of sine wave, spike wave and square wave; A pair of annular medical zinc alloy electrodes electrically connected to the waveform output module; The program control module presets the waveform cycle timing as described in claim 2.

7. The skin care method based on regulating aquaporins by multi-waveform intermediate frequency electrical signals according to claim 6, characterized in that The annular medical zinc alloy electrode is a replaceable structure, and the electrode surface is covered with a biocompatible conductive coating.

8. The skin care method based on regulating aquaporins by multi-waveform intermediate frequency electrical signals according to claim 6, characterized in that ,characterized in that, Equipped with an impedance feedback unit to dynamically adjust the current output power according to skin resistance.

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