Method for promoting microbial activity by using vitamin B12 mushroom dreg fertilizer

Abstract

The invention relates to the technical field of fertilizers, in particular to a method for promoting microbial activity by using a vitamin B12 mushroom dreg fertilizer, which comprises the following steps: acquiring vitamin B12 mushroom dregs, and pretreating the vitamin B12 mushroom dregs; adding a modified additive into the vitamin B12 mushroom dregs, and stirring; inoculating a fermentation strain into the mixed material, and putting the inoculated material into a fermentation tank for fermentation; after the fermentation is finished, drying the materials, and granulating to prepare the granular vitamin B12 mushroom dreg fertilizer; the vitamin B12 mushroom dreg fertilizer is uniformly spread on the surface of field soil, then ploughing is performed, and the mushroom dreg fertilizer can be dressed timely according to the crop growth condition and the soil fertility condition. According to the application, the vitamin B12 mushroom dregs are pretreated, the modified additive is added and stirred, then the fermentation strain is inoculated for fermentation, and finally the granular vitamin B12 mushroom dreg fertilizer is prepared, so that the soil fertility can be effectively improved, the crop growth can be promoted, and the fertilizer can be flexibly dressed according to crop requirements and soil conditions.

Description

Technical Field

[0001] The invention relates to the field of fertilizers, and in particular to a method for promoting microbial activity using vitamin B12 bacterial residue fertilizer. Background Art

[0002] Highly active soil microorganisms play a vital role in agricultural production. They not only guarantee soil fertility but also serve as the cornerstone of nutrient cycling and healthy plant growth. These microorganisms accelerate the decomposition of organic matter, releasing nutrients needed by plants while also optimizing soil structure and enhancing its ability to retain nutrients and water. However, while traditional chemical fertilizers can quickly replenish crop nutrients in the short term, long-term overreliance on them can damage soil microbial communities, leading to adverse consequences such as soil compaction.

[0003] Vitamin B12 residue, a by-product of the vitamin B12 production process, is rich in protein, polysaccharides, and a variety of trace elements, and has great potential for conversion into fertilizers that promote microbial activity. If this resource can be effectively utilized, it will not only achieve the resource recycling of waste, but also provide a new fertilizer option for the agricultural sector that is both environmentally friendly and sustainable. However, the current utilization methods of vitamin B12 residue are relatively limited, and most of the residue is treated as waste, which not only causes a huge waste of resources, but also may cause pollution to the environment.

[0004] In the process of preparing fertilizers that efficiently promote microbial activity, the low efficiency of ingredient extraction and conversion is a problem that needs to be solved urgently. This leads to a large loss of ingredients during the fertilizer preparation process, affecting the final effect of the fertilizer. Secondly, due to the complex composition of vitamin B12 residue, it is relatively difficult to control the type, quantity, activity and environmental conditions of microorganisms during the fermentation process, which may lead to poor fermentation results and thus affect the quality and effect of the fertilizer. In addition, unstable product quality is also a problem currently faced in the preparation of vitamin B12 residue fertilizers. Due to the influence of various factors in the preparation process, the quality of fertilizer products is often uneven, which brings certain risks to agricultural production. Summary of the Invention

[0005] The present application provides a method for promoting microbial activity by using vitamin B12 bacterial residue fertilizer, so as to solve the problems of low component extraction and conversion efficiency, low microbial fermentation activity and unstable product quality in related technologies.

[0006] In order to achieve the above-mentioned object, the technical solution adopted by the present invention is as follows: The present invention provides a method for promoting microbial activity by using vitamin B12 fungus residue fertilizer, comprising the following steps: obtaining vitamin B12 fungus residue, pre-treating the vitamin B12 fungus residue, wherein the pre-treatment includes drying the vitamin B12 fungus residue to reduce its moisture content to 10%-15%, and then crushing it to obtain a uniform fungus residue powder; adding a modifying additive to the vitamin B12 fungus residue and stirring it for 30-60 minutes to fully mix it; The mixed material is inoculated with fermentation bacteria, and the inoculated material is placed in a fermentation tank for fermentation. The fermentation temperature is controlled at 30-35°C, the humidity is controlled at 60%-70%, and the fermentation time is 5-7 days. After the fermentation is completed, the material is dried to reduce its moisture content to below 20%, and then the dried material is granulated to prepare granular vitamin B12 fungus residue fertilizer. The vitamin B12 fungus residue fertilizer is evenly spread on the surface of the field soil, and then plowed to fully mix the fungus residue fertilizer with the soil. The fungus residue fertilizer can be applied in time according to the crop growth and soil fertility.

[0007] Furthermore, the modifying additive is composed of the following weight ratios: Humic acid: 5-10 parts; Amino acids: 10-18 parts; Organic chelating agent: 3-6 parts; Cobalt sulfide: 1-3 parts; Microbial agent: 5-10 parts.

[0008] Furthermore, the organic chelating agent is ethoxybenzyldiethylenetriaminepentaacetic acid.

[0009] Furthermore, the preparation process of the ethoxybenzyldiethylenetriaminepentaacetic acid is as follows: The pH value of diethylenetriamine is adjusted to a range of 5-10, and a two-step reaction is carried out with a hydroxyacetonitrile aqueous solution at a molar ratio of 1.5-3.0:1 and 2.5-6:1 at a specific temperature to generate an intermediate product, diethylenetriamine pentaacetonitrile; converting the intermediate product into diethylenetriamine pentaacetic acid or a hydrolyzate of diethylenetriamine pentaacetic acid salt through a hydrolysis reaction, adding activated carbon to the hydrolyzate for decolorization and performing post-treatment to obtain solid diethylenetriamine pentaacetic acid; After obtaining diethylenetriamine pentaacetic acid, it reacts with a reagent containing ethoxybenzyl to form ethoxybenzyldiethylenetriamine pentaacetic acid.

[0010] Furthermore, activated carbon is added to the hydrolyzate for decolorization and post-treatment, including: Determine whether the product is a salt or an acid. If it is a salt, convert it into diethylenetriaminepentaacetic acid by passing it through an ion exchange resin. Otherwise, remove the excess solvent by evaporation and concentration, then cool and crystallize, and collect the solid diethylenetriaminepentaacetic acid using a filtration device and dry it in an oven to constant weight.

[0011] Furthermore, the microbial agent is a photosynthetic bacterial agent.

[0012] Furthermore, the fermentation process also includes regular turning of the pile, wherein the turning frequency is 1-2 times a day, and the depth of each turning is 1 / 3-1 / 2 of the total height of the material.

[0013] Further, the dried material is granulated, comprising: By adding a binder and controlling the speed and temperature of the granulator, a uniform granular product can be obtained; The granular product is screened and dried, and then a first layer of membrane solution is prepared and evenly impregnated on the surface of the granules to volatilize the organic solvent and form a firm first layer of membrane; A second layer of film is prepared again, and the particles wrapped with the first layer of film are wrapped with the second layer of film, and then dried to obtain an ethoxybenzyldiethylenetriaminepentaacetic acid particle product wrapped with a double layer of film.

[0014] Furthermore, the first layer membrane solution comprises the following components in parts by weight: Polyethylene: 50-70 parts; Cyclohexane: 30-50 parts; Zinc oxide: 1-5 parts; Citrate: 5-15 parts.

[0015] Furthermore, the second layer membrane solution comprises the following components in parts by weight: Polylactic acid: 60-80 parts; Sodium alginate: 10-20 parts; Ferric chloride: 1-3 parts; Auxin: 0.1-3 parts.

[0016] The beneficial effects achieved by adopting the above-mentioned present invention are as follows: 1. The present invention prepares a method for promoting microbial activity by using vitamin B12 bacterial residue fertilizer. The method comprises the following steps: finely adjusting the pH value to optimize the nucleophilic activity of the nitrogen atom in diethylenetriamine, promoting a step-by-step, selective nucleophilic substitution reaction between the nitrogen atom in diethylenetriamine and hydroxyacetonitrile, and forming an intermediate product containing an acetonitrile group. Subsequently, under alkaline conditions, the intermediate product undergoes a hydrolysis process, and the cyano group is converted into a carboxyl group to generate diethylenetriamine pentaacetic acid or its diacetate. Finally, utilizing the nucleophilicity of the carboxyl group, a nucleophilic substitution reaction occurs with an ethoxybenzyl halide, successfully introducing the ethoxybenzyl group, and completing the synthesis of the ethoxybenzyl diethylenetriamine pentaacetic acid. Secondly, the ethoxybenzyl diethylenetriamine pentaacetic acid, as an organic chelating agent, has a strong chelating ability and can form chelates with trace elements in soil, thereby improving the solubility and bioavailability of the trace elements. Specifically, the chelate formed by ethoxybenzyldiethylenetriaminepentaacetic acid and iron ions, in which the carboxyl oxygen atom and nitrogen atom in the molecule provide lone pairs of electrons, forming multiple coordination bonds with the iron ions, encapsulating the iron ions in the center of the chelate, and converting the metal ions from a free state that easily forms insoluble precipitates to a stable chelated state. This prevents the metal ions from combining with hydroxide ions, phosphate ions, etc. in the soil to form insoluble precipitates, thereby improving the solubility and bioavailability of the metal ions, facilitating their absorption and utilization by soil microorganisms and plant roots, and enhancing the activity of microorganisms. 2. At the same time, after being absorbed by microorganisms, the metal ions chelated by ethoxybenzyldiethylenetriaminepentaacetic acid can activate microbial agents, accelerate the decomposition and transformation of organic matter in fertilizers by microorganisms, provide more energy and nutrients for microbial growth and reproduction, thereby enhancing microbial activity and promoting material circulation and energy flow in the soil ecosystem; humic acid increases the soil's cation exchange capacity and nutrient adsorption capacity through its active functional groups, while also improving soil structure; amino acids provide a nitrogen source and metabolic support for microorganisms; and cobalt ions are essential elements in the synthesis of vitamin B12. The addition of cobalt sulfide promotes the activity of enzymes related to vitamin B12 synthesis in microorganisms, thereby affecting the metabolic pathways and growth and reproduction of microorganisms. In addition, cobalt sulfide may also affect the structure and function of microbial cell membranes, improving the microorganism's ability to adapt to the environment; photosynthetic bacterial agents provide carbon sources, fix nitrogen, and produce bioactive substances through photosynthesis, promoting microbial growth and reproduction. These components work synergistically to optimize the soil environment and enhance microbial activity. 3. The present invention proposes a double-layer membrane design in the granulation process, wherein the first layer of the membrane is composed of polyethylene, cyclohexane, zinc oxide and citrate, which provides strength, stability, uniform dispersion, antibacterial and antiseptic properties and flexibility. At the same time, the polymer network structure formed by polyethylene and the interaction between the various components make it possible for nutrients to diffuse out through the pores of the membrane at a slower rate, thereby achieving a slow release of nutrients and providing a long-lasting and stable nutrient supply for soil microorganisms; the second layer of the membrane is mainly composed of polylactic acid, supplemented by sodium alginate, ferric chloride and auxin, to achieve the functions of biodegradation, long-term sustained release, intelligent regulation of stability and promotion of plant root growth.

[0017] 4. The double-layer membrane structure formed by the present invention provides a double protective barrier for fertilizers, greatly reducing the loss of fertilizer nutrients during storage and transportation. At the same time, the different structures and degradation characteristics of the two layers of membranes realize precise slow-release control of fertilizer nutrients. The first layer of membrane first plays an initial slow-release role. As time goes by, the second layer of membrane begins to degrade, continuously providing nutrients to soil microorganisms, forming a multi-level, continuous and stable nutrient supply system; secondly, it can better adapt to different soil environments. Under different soil pH, humidity and microbial community conditions, the double-layer membrane can ensure the stability of the fertilizer and the effectiveness of nutrient release by changing its own physical and chemical properties; 5. The present invention utilizes vitamin B12 fungus residue as the main raw material to prepare fertilizer, thus realizing resource utilization of waste, reducing the cost of fungus residue treatment, and reducing dependence on traditional fertilizer raw materials, thereby reducing the production cost of fertilizer. On the other hand, since the fertilizer can significantly enhance soil microbial activity, improve soil quality, promote crop growth, enhance crop resistance and reduce the occurrence of pests and diseases, the crop yield is increased and the quality is improved. This means that with the same planting area, more and better quality agricultural products can be harvested, thereby improving the economic benefits of agricultural production. Moreover, the amount of pesticides required for pest and disease control, as well as the cost of other agricultural production materials that are additionally invested due to soil improvement and poor crop growth, are reduced. In addition, the precise slow-release control of the fertilizer reduces the frequency and amount of fertilizer use, further reducing the fertilizer cost in agricultural production. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The above and / or additional aspects and advantages of the present application will become apparent and easily understood from the following description of the embodiments in conjunction with the accompanying drawings, in which: Figure 1 A flow chart of a method for promoting microbial activity using a vitamin B12 bacterial residue fertilizer according to one embodiment of the present invention; Figure 2 A schematic diagram of a vitamin B12 bacterial residue fertilizer provided in one embodiment of the present invention. DETAILED DESCRIPTION

[0019] The technical solution of the present invention is described below through specific examples. It should be understood that the one or more method steps mentioned in the present invention do not exclude the presence of other method steps before and after the combination step or the insertion of other method steps between these explicitly mentioned steps; it should also be understood that these embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention. Moreover, unless otherwise specified, the numbering of each method step is only a convenient tool for identifying each method step, and is not intended to limit the order of arrangement of each method step or to define the scope of the present invention. Changes or adjustments in their relative relationships, without substantially changing the technical content, should also be regarded as the scope of the present invention.

[0020] In order to better understand the above technical solutions, exemplary embodiments of the present invention are described in more detail below. Although exemplary embodiments of the present invention are shown, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0021] The following describes a method for promoting microbial activity by using vitamin B12 fungus residue fertilizer according to an embodiment of the present application with reference to the accompanying drawings. In response to the problem of difficult fermentation control mentioned in the above background technology, the present application provides a method for promoting microbial activity by using vitamin B12 fungus residue fertilizer. In this method, ethoxybenzyldiethylenetriaminepentaacetic acid is finely synthesized as an organic chelating agent to optimize soil trace element utilization, and a variety of beneficial ingredients are combined to jointly improve microbial activity and soil quality. At the same time, a double-layer membrane design is adopted to achieve precise slow-release control of fertilizer nutrients, reduce nutrient loss, and adapt to different soil environments. By using vitamin B12 fungus residue as the main raw material, waste resource utilization is realized, costs are reduced, the economic benefits of agricultural production are improved, and the use of pesticides and fertilizers is reduced. Thus, the problems of low component extraction and conversion efficiency, low microbial fermentation activity, and unstable product quality in the related art are solved.

[0022] Specifically, Figure 1 A method for promoting microbial activity by using vitamin B12 bacterial residue fertilizer is provided in an embodiment of the present application.

[0023] like Figure 1 As shown, the method for promoting microbial activity by using vitamin B12 bacterial residue fertilizer comprises the following steps: In step S101, vitamin B12 bacterial residue is obtained and pre-processed.

[0024] Among them, the pretreatment includes drying the vitamin B12 mushroom residue to reduce its moisture content to 10%-15%, and then crushing it to obtain uniform mushroom residue powder.

[0025] It can be understood that the embodiment of the present application obtains vitamin B12 bacterial residue and performs pretreatment, which can not only achieve waste recycling and reduce environmental pollution, but also provide convenience for subsequent processing.

[0026] In step S102, a modification additive is added to the vitamin B12 residue and stirred for 30-60 minutes to ensure that the mixture is fully mixed.

[0027] It can be understood that the embodiment of the present application achieves improvement in the performance of the vitamin B12 residue and enhancement of its utilization value by adding a modifying additive to the vitamin B12 residue and stirring it for 30-60 minutes, thereby promoting its resource utilization and environmental benefits.

[0028] In the embodiment of the present application, the modifying additive is composed of the following weight ratios: humic acid: 5-10 parts; amino acid: 10-18 parts; organic chelating agent: 3-6 parts; cobalt sulfide: 1-3 parts; microbial agent: 5-10 parts.

[0029] The organic chelating agent is ethoxybenzyldiethylenetriaminepentaacetic acid, and the microbial agent is a photosynthetic bacterial agent.

[0030] It is understandable that humic acid in the present application embodiment enhances the cohesion and structure of the soil through its abundant functional groups, improves air permeability and water retention, and promotes the effectiveness of trace metal elements. Amino acids not only provide nitrogen sources for plants, but also participate in physiological processes in plants, such as protein synthesis and enzyme catalysis, and regulate the effectiveness of nutrients in the soil through coordination. Ethoxybenzyldiethylenetriaminepentaacetic acid, by virtue of its strong chelating ability, can stabilize heavy metal ions in the soil and essential trace elements for plants, thereby improving the utilization efficiency of nutrients. Cobalt sulfide may catalyze the redox reaction in the soil, participate in the composition or activation of plant enzymes, affect the soil microbial community, and thus indirectly promote plant growth. Photosynthetic bacterial agents increase soil permeability through photosynthesis, improve the redox environment, secrete plant growth promoting substances, decompose organic matter to improve soil fertility, and may form a symbiotic relationship with other microorganisms, significantly improve the soil environment, promote plant growth, improve soil fertility, and contribute to the fixation of heavy metals and the absorption and utilization of essential elements for plants.

[0031] In an embodiment of the present application, the preparation process of ethoxybenzyldiethylenetriaminepentaacetic acid is as follows: adjusting the pH value of diethylenetriamine to a range of 5-10, and reacting it with an aqueous solution of hydroxyacetonitrile in a two-step reaction at a specific temperature in a molar ratio of 1.5-3.0:1 and 2.5-6:1 to produce an intermediate product, diethylenetriaminepentaacetonitrile; converting the intermediate product into diethylenetriaminepentaacetic acid or a hydrolyzate of diethylenetriaminepentaacetic acid salt through a hydrolysis reaction, adding activated carbon to the hydrolyzate for decolorization and performing post-treatment to obtain solid diethylenetriaminepentaacetic acid; and reacting the obtained diethylenetriaminepentaacetic acid with a reagent containing an ethoxybenzyl group to form ethoxybenzyldiethylenetriaminepentaacetic acid.

[0032] The specific temperature is a preset temperature, such as 50-100°C.

[0033] It will be appreciated that in the examples of the present application, the pH value of diethylenetriamine is adjusted to a suitable range of 5-10, and then reacted with an aqueous solution of hydroxyacetonitrile in two steps at different molar ratios under specific temperature conditions to produce the intermediate product, diethylenetriamine pentaacetonitrile. Subsequently, the intermediate product is converted into a hydrolyzed solution of diethylenetriamine pentaacetic acid or a salt thereof through a hydrolysis reaction. To improve the purity and appearance of the product, activated carbon is added to the hydrolyzed solution for decolorization, and subsequent post-processing steps are performed to obtain diethylenetriamine pentaacetic acid in solid form. Finally, the resulting solid diethylenetriamine pentaacetic acid is reacted with a reagent containing an ethoxybenzyl group to successfully prepare ethoxybenzyl diethylenetriamine pentaacetic acid, which has excellent chelating properties and can effectively complex metal ions, thereby improving its stability and bioavailability in specific applications.

[0034] In an embodiment of the present application, activated carbon is added to the hydrolyzate for decolorization and post-treatment, including: determining whether the product is a salt or an acid. If it is a salt, it is converted into diethylenetriaminepentaacetic acid through an ion exchange resin; otherwise, the excess solvent is removed by evaporation and concentration, followed by cooling and crystallization, and then the solid diethylenetriaminepentaacetic acid is collected using a filtration device and dried in an oven to constant weight.

[0035] It can be understood that the embodiments of the present application can effectively achieve the preparation and purification of diethylenetriaminepentaacetic acid by determining whether the product is a salt or an acid and taking corresponding ion exchange resin conversion or evaporation concentration, cooling crystallization, filtration, drying and other operating procedures, thereby improving the purity and quality of the product and ensuring the effective acquisition of diethylenetriaminepentaacetic acid.

[0036] In step S103, fermentation bacteria are inoculated into the mixed material, and the inoculated material is placed in a fermentation tank for fermentation. The fermentation temperature is controlled at 30-35°C, the humidity is controlled at 60%-70%, and the fermentation time is 5-7 days.

[0037] In the embodiment of the present application, the fermentation process also includes regular compost turning.

[0038] The frequency of turning the pile is 1-2 times a day, and the depth of each turning is 1 / 3-1 / 2 of the total height of the material.

[0039] It can be understood that the embodiment of the present application is to inoculate the fermentation bacteria into the mixed material, and carry out a fermentation treatment for 5-7 days in a fermentation tank under suitable temperature and humidity conditions, while turning the pile 1-2 times a day, and each turning depth is 1 / 3-1 / 2 of the material height to promote uniform fermentation of the material and improve fermentation efficiency and quality.

[0040] In step S104, after the fermentation is completed, the material is dried to reduce its moisture content to below 20%, and then the dried material is granulated to produce granular vitamin B12 fungus residue fertilizer.

[0041] In an embodiment of the present application, the dried material is granulated, including: preparing a uniform granular product by adding a binder and controlling the speed and temperature of the granulator; screening and drying the granular product, and then preparing a first layer of membrane solution, and evenly impregnating it on the surface of the particles, allowing the organic solvent to evaporate to form a strong first layer of membrane; preparing a second layer of membrane again, and wrapping the particles wrapped with the first layer of membrane with the second layer of membrane, and then drying the particles to obtain a double-layer membrane-wrapped ethoxybenzyldiethylenetriaminepentaacetic acid granular product.

[0042] Among them, Figure 2 As shown, the first layer membrane solution comprises the following components in parts by weight: polyethylene: 50-70 parts; cyclohexane: 30-50 parts; zinc oxide: 1-5 parts; citrate: 5-15 parts.

[0043] Among them, Figure 2 As shown, the second layer membrane solution comprises the following components in parts by weight: polylactic acid: 60-80 parts; sodium alginate: 10-20 parts; ferric chloride: 1-3 parts; and auxin: 0.1-3 parts.

[0044] It is understood that the embodiments of the present application convert vitamin B12 bacterial residue into a highly effective fertilizer through fermentation, drying, granulation, and double-layer film coating. By precisely controlling the granulation process and the ratio of the double-layer film solution, a uniform granular fertilizer with excellent stability and slow-release properties is produced. This not only reduces the moisture content of the material and improves the storage and use efficiency of the fertilizer, but also enhances the fertilizer's resistance to decomposition and environmental adaptability through the protection of the double-layer film, achieving efficient resource utilization and environmentally friendly protection.

[0045] In step S105, the vitamin B12 fungus residue fertilizer is evenly spread on the field soil surface, and then the soil is plowed to fully mix the fungus residue fertilizer with the soil. According to the crop growth and soil fertility, the fungus residue fertilizer can be topdressed in time.

[0046] It is understood that the present embodiment achieves full integration of the fungus residue fertilizer with the soil by evenly spreading and plowing the vitamin B12 fungus residue fertilizer into the field soil, which not only improves soil fertility but also promotes the healthy growth of crops. The timely application of fungus residue fertilizer based on crop growth needs and soil fertility further ensures the nutrient supply of crops, improves fertilizer utilization and agricultural production efficiency.

[0047] According to the present application, a method for promoting microbial activity by using vitamin B12 fungus residue fertilizer is proposed. By finely synthesizing ethoxybenzyldiethylenetriaminepentaacetic acid as an organic chelating agent, the utilization of trace elements in the soil is optimized, and a variety of beneficial ingredients are combined to jointly improve microbial activity and soil quality. At the same time, a double-layer membrane design is adopted to achieve precise slow-release control of fertilizer nutrients, reduce nutrient loss, and adapt to different soil environments. By using vitamin B12 fungus residue as the main raw material, waste resource utilization is realized, costs are reduced, the economic benefits of agricultural production are improved, and the use of pesticides and fertilizers is reduced. As a result, the problems of low component extraction and conversion efficiency, low microbial fermentation activity, and unstable product quality in the related technologies are solved.

[0048] The present invention will be further described below with reference to the following examples.

[0049] Example 1 The invention provides a method for promoting microbial activity by using vitamin B12 fungus residue fertilizer. The method comprises the following steps: obtaining vitamin B12 fungus residue, pre-treating the vitamin B12 fungus residue, wherein the pre-treatment comprises drying the vitamin B12 fungus residue to reduce its moisture content to 10%-15%, and then pulverizing the vitamin B12 fungus residue to obtain uniform fungus residue powder; adding a modifying additive to the vitamin B12 fungus residue and stirring the mixture for 30-60 minutes to fully and evenly mix the mixture; inoculating fermentation bacteria into the mixed material, placing the inoculated material into a fermentation tank for fermentation, controlling the fermentation temperature to 30-35°C and the humidity to 60%-70% for 5-7 days; after the fermentation is completed, drying the material to reduce its moisture content to below 20%, and then granulating the dried material to prepare granular vitamin B12 fungus residue fertilizer; evenly spreading the vitamin B12 fungus residue fertilizer on the surface of field soil, and then plowing the soil to fully mix the fungus residue fertilizer with the soil, and applying the fungus residue fertilizer in a timely manner according to crop growth conditions and soil fertility conditions.

[0050] In the examples of the present application, the modifying additives are composed in the following weight ratios: Humic acid: 5 parts; Amino acids: 10 parts; Organic chelating agent: 3 parts; Cobalt sulfide: 1 part; Microbial agent: 5 parts.

[0051] In the embodiment of the present application, the organic chelating agent is ethoxybenzyldiethylenetriaminepentaacetic acid.

[0052] In the examples of the present application, the preparation process of ethoxybenzyldiethylenetriaminepentaacetic acid is as follows: The pH value of diethylenetriamine is adjusted to the range of 5-10, and a two-step reaction is carried out with an aqueous solution of hydroxyacetonitrile at a specific temperature in a molar ratio of 1.5:1 and 2.5:1 to produce an intermediate product, diethylenetriamine pentaacetonitrile; converting the intermediate product into diethylenetriamine pentaacetic acid or a hydrolyzate of diethylenetriamine pentaacetic acid salt through a hydrolysis reaction, adding activated carbon to the hydrolyzate for decolorization and performing post-treatment to obtain solid diethylenetriamine pentaacetic acid; After obtaining diethylenetriamine pentaacetic acid, it reacts with a reagent containing ethoxybenzyl to form ethoxybenzyldiethylenetriamine pentaacetic acid.

[0053] In the embodiment of the present application, activated carbon is added to the hydrolyzate for decolorization and post-treatment, including: Determine whether the product is a salt or an acid. If it is a salt, convert it into diethylenetriaminepentaacetic acid by passing it through an ion exchange resin. Otherwise, remove the excess solvent by evaporation and concentration, then cool and crystallize, and collect the solid diethylenetriaminepentaacetic acid using a filtration device and dry it in an oven to constant weight.

[0054] In the embodiment of the present application, the microbial agent is a photosynthetic bacterial agent.

[0055] In the embodiment of the present application, the fermentation process also includes regular turning of the pile, wherein the turning frequency is 1-2 times a day, and the depth of each turning is 1 / 3-1 / 2 of the total height of the material.

[0056] In the embodiment of the present application, the dried material is granulated, comprising: By adding a binder and controlling the speed and temperature of the granulator, a uniform granular product can be obtained; The granular product is screened and dried, and then the first layer of membrane solution is prepared and evenly impregnated on the surface of the granules to allow the organic solvent to evaporate and form a firm first layer of membrane; A second layer of film is prepared again, and the particles wrapped with the first layer of film are wrapped with the second layer of film, and then dried to obtain an ethoxybenzyldiethylenetriaminepentaacetic acid particle product wrapped with a double layer of film.

[0057] In the embodiment of the present application, the first layer membrane solution comprises the following components in parts by weight: Polyethylene: 50 parts; Cyclohexane: 30 parts; Zinc oxide: 1 part; Citrate ester: 5 parts.

[0058] In the embodiment of the present application, the second layer membrane solution comprises the following components in parts by weight: Polylactic acid: 60 parts; Sodium alginate: 10 parts; Ferric chloride: 1 part; Auxin: 0.1 parts.

[0059] Example 2 The invention provides a method for promoting microbial activity by using vitamin B12 fungus residue fertilizer. The method comprises the following steps: obtaining vitamin B12 fungus residue, pre-treating the vitamin B12 fungus residue, wherein the pre-treatment comprises drying the vitamin B12 fungus residue to reduce its moisture content to 10%-15%, and then pulverizing the vitamin B12 fungus residue to obtain uniform fungus residue powder; adding a modifying additive to the vitamin B12 fungus residue and stirring the mixture for 30-60 minutes to fully and evenly mix the mixture; inoculating fermentation bacteria into the mixed material, placing the inoculated material into a fermentation tank for fermentation, controlling the fermentation temperature to 30-35°C and the humidity to 60%-70% for 5-7 days; after the fermentation is completed, drying the material to reduce its moisture content to below 20%, and then granulating the dried material to prepare granular vitamin B12 fungus residue fertilizer; evenly spreading the vitamin B12 fungus residue fertilizer on the surface of field soil, and then plowing the soil to fully mix the fungus residue fertilizer with the soil, and applying the fungus residue fertilizer in a timely manner according to crop growth conditions and soil fertility conditions.

[0060] In the examples of the present application, the modifying additives are composed in the following weight ratios: Humic acid: 6 parts; Amino acids: 12 parts; Organic chelating agent: 4 parts; Cobalt sulfide: 1.5 parts; Microbial agent: 6 parts.

[0061] In the embodiment of the present application, the organic chelating agent is ethoxybenzyldiethylenetriaminepentaacetic acid.

[0062] In the examples of the present application, the preparation process of ethoxybenzyldiethylenetriaminepentaacetic acid is as follows: The pH value of diethylenetriamine is adjusted to the range of 5-10, and a two-step reaction is carried out with an aqueous solution of hydroxyacetonitrile at a specific temperature in a molar ratio of 1.8:1 and 3.5:1 to produce an intermediate product, diethylenetriamine pentaacetonitrile; converting the intermediate product into diethylenetriamine pentaacetic acid or a hydrolyzate of diethylenetriamine pentaacetic acid salt through a hydrolysis reaction, adding activated carbon to the hydrolyzate for decolorization and performing post-treatment to obtain solid diethylenetriamine pentaacetic acid; After obtaining diethylenetriamine pentaacetic acid, it reacts with a reagent containing ethoxybenzyl to form ethoxybenzyldiethylenetriamine pentaacetic acid.

[0063] In the embodiment of the present application, activated carbon is added to the hydrolyzate for decolorization and post-treatment, including: Determine whether the product is a salt or an acid. If it is a salt, convert it into diethylenetriaminepentaacetic acid by passing it through an ion exchange resin. Otherwise, remove the excess solvent by evaporation and concentration, then cool and crystallize, and collect the solid diethylenetriaminepentaacetic acid using a filtration device and dry it in an oven to constant weight.

[0064] In the embodiment of the present application, the microbial agent is a photosynthetic bacterial agent.

[0065] In the embodiment of the present application, the fermentation process also includes regular turning of the pile, wherein the turning frequency is 1-2 times a day, and the depth of each turning is 1 / 3-1 / 2 of the total height of the material.

[0066] In the embodiment of the present application, the dried material is granulated, comprising: By adding a binder and controlling the speed and temperature of the granulator, a uniform granular product can be obtained; The granular product is screened and dried, and then the first layer of membrane solution is prepared and evenly impregnated on the surface of the granules to allow the organic solvent to evaporate and form a firm first layer of membrane; A second layer of film is prepared again, and the particles wrapped with the first layer of film are wrapped with the second layer of film, and then dried to obtain an ethoxybenzyldiethylenetriaminepentaacetic acid particle product wrapped with a double layer of film.

[0067] In the embodiment of the present application, the first layer membrane solution comprises the following components in parts by weight: Polyethylene: 55 parts; Cyclohexane: 35 parts; Zinc oxide: 2 parts; Citrate ester: 8 parts.

[0068] In the embodiment of the present application, the second layer membrane solution comprises the following components in parts by weight: Polylactic acid: 65 parts; Sodium alginate: 12 parts; Ferric chloride: 1.5 parts; Auxin: 1 part.

[0069] Example 3 The invention provides a method for promoting microbial activity by using vitamin B12 fungus residue fertilizer. The method comprises the following steps: obtaining vitamin B12 fungus residue, pre-treating the vitamin B12 fungus residue, wherein the pre-treatment comprises drying the vitamin B12 fungus residue to reduce its moisture content to 10%-15%, and then pulverizing the vitamin B12 fungus residue to obtain uniform fungus residue powder; adding a modifying additive to the vitamin B12 fungus residue and stirring the mixture for 30-60 minutes to fully and evenly mix the mixture; inoculating fermentation bacteria into the mixed material, placing the inoculated material into a fermentation tank for fermentation, controlling the fermentation temperature to 30-35°C and the humidity to 60%-70% for 5-7 days; after the fermentation is completed, drying the material to reduce its moisture content to below 20%, and then granulating the dried material to prepare granular vitamin B12 fungus residue fertilizer; evenly spreading the vitamin B12 fungus residue fertilizer on the surface of field soil, and then plowing the soil to fully mix the fungus residue fertilizer with the soil, and applying the fungus residue fertilizer in a timely manner according to crop growth conditions and soil fertility conditions.

[0070] In the examples of the present application, the modifying additives are composed in the following weight ratios: Humic acid: 8 parts; Amino acids: 15 parts; Organic chelating agent: 5 parts; Cobalt sulfide: 2 parts; Microbial agent: 8 parts.

[0071] In the embodiment of the present application, the organic chelating agent is ethoxybenzyldiethylenetriaminepentaacetic acid.

[0072] In the examples of the present application, the preparation process of ethoxybenzyldiethylenetriaminepentaacetic acid is as follows: The pH value of diethylenetriamine is adjusted to the range of 5-10, and a two-step reaction is carried out with a hydroxyacetonitrile aqueous solution at a molar ratio of 2:1 and 4.5:1 at a specific temperature to produce an intermediate product, diethylenetriamine pentaacetonitrile; converting the intermediate product into diethylenetriamine pentaacetic acid or a hydrolyzate of diethylenetriamine pentaacetic acid salt through a hydrolysis reaction, adding activated carbon to the hydrolyzate for decolorization and performing post-treatment to obtain solid diethylenetriamine pentaacetic acid; After obtaining diethylenetriamine pentaacetic acid, it reacts with a reagent containing ethoxybenzyl to form ethoxybenzyldiethylenetriamine pentaacetic acid.

[0073] In the embodiment of the present application, activated carbon is added to the hydrolyzate for decolorization and post-treatment, including: Determine whether the product is a salt or an acid. If it is a salt, convert it into diethylenetriaminepentaacetic acid by passing it through an ion exchange resin. Otherwise, remove the excess solvent by evaporation and concentration, then cool and crystallize, and collect the solid diethylenetriaminepentaacetic acid using a filtration device and dry it in an oven to constant weight.

[0074] In the embodiment of the present application, the microbial agent is a photosynthetic bacterial agent.

[0075] In the embodiment of the present application, the fermentation process also includes regular turning of the pile, wherein the turning frequency is 1-2 times a day, and the depth of each turning is 1 / 3-1 / 2 of the total height of the material.

[0076] In the embodiment of the present application, the dried material is granulated, comprising: By adding a binder and controlling the speed and temperature of the granulator, a uniform granular product can be obtained; The granular product is screened and dried, and then the first layer of membrane solution is prepared and evenly impregnated on the surface of the granules to allow the organic solvent to evaporate and form a firm first layer of membrane; A second layer of film is prepared again, and the particles wrapped with the first layer of film are wrapped with the second layer of film, and then dried to obtain an ethoxybenzyldiethylenetriaminepentaacetic acid particle product wrapped with a double layer of film.

[0077] In the embodiment of the present application, the first layer membrane solution comprises the following components in parts by weight: Polyethylene: 60 parts; Cyclohexane: 40 parts; Zinc oxide: 3 parts; Citrate ester: 10 parts.

[0078] In the embodiment of the present application, the second layer membrane solution comprises the following components in parts by weight: Polylactic acid: 70 parts; Sodium alginate: 15 parts; Ferric chloride: 2 parts; Auxin: 2 parts.

[0079] Example 4 The invention provides a method for promoting microbial activity by using vitamin B12 fungus residue fertilizer. The method comprises the following steps: obtaining vitamin B12 fungus residue, pre-treating the vitamin B12 fungus residue, wherein the pre-treatment comprises drying the vitamin B12 fungus residue to reduce its moisture content to 10%-15%, and then pulverizing the vitamin B12 fungus residue to obtain uniform fungus residue powder; adding a modifying additive to the vitamin B12 fungus residue and stirring the mixture for 30-60 minutes to fully and evenly mix the mixture; inoculating fermentation bacteria into the mixed material, placing the inoculated material into a fermentation tank for fermentation, controlling the fermentation temperature to 30-35°C and the humidity to 60%-70% for 5-7 days; after the fermentation is completed, drying the material to reduce its moisture content to below 20%, and then granulating the dried material to prepare granular vitamin B12 fungus residue fertilizer; evenly spreading the vitamin B12 fungus residue fertilizer on the surface of field soil, and then plowing the soil to fully mix the fungus residue fertilizer with the soil, and applying the fungus residue fertilizer in a timely manner according to crop growth conditions and soil fertility conditions.

[0080] In the examples of the present application, the modifying additives are composed in the following weight ratios: Humic acid: 9 parts; Amino acids: 16 parts; Organic chelating agent: 5 parts; Cobalt sulfide: 2.5 parts; Microbial agent: 8 parts.

[0081] In the embodiment of the present application, the organic chelating agent is ethoxybenzyldiethylenetriaminepentaacetic acid.

[0082] In the examples of the present application, the preparation process of ethoxybenzyldiethylenetriaminepentaacetic acid is as follows: The pH value of diethylenetriamine is adjusted to the range of 5-10, and a two-step reaction is carried out with a hydroxyacetonitrile aqueous solution at a molar ratio of 2.5:1 and 5:1 at a specific temperature to produce an intermediate product, diethylenetriamine pentaacetonitrile; converting the intermediate product into diethylenetriamine pentaacetic acid or a hydrolyzate of diethylenetriamine pentaacetic acid salt through a hydrolysis reaction, adding activated carbon to the hydrolyzate for decolorization and performing post-treatment to obtain solid diethylenetriamine pentaacetic acid; After obtaining diethylenetriamine pentaacetic acid, it reacts with a reagent containing ethoxybenzyl to form ethoxybenzyldiethylenetriamine pentaacetic acid.

[0083] In the embodiment of the present application, activated carbon is added to the hydrolyzate for decolorization and post-treatment, including: Determine whether the product is a salt or an acid. If it is a salt, convert it into diethylenetriaminepentaacetic acid by passing it through an ion exchange resin. Otherwise, remove the excess solvent by evaporation and concentration, then cool and crystallize, and collect the solid diethylenetriaminepentaacetic acid using a filtration device and dry it in an oven to constant weight.

[0084] In the embodiment of the present application, the microbial agent is a photosynthetic bacterial agent.

[0085] In the embodiment of the present application, the fermentation process also includes regular turning of the pile, wherein the turning frequency is 1-2 times a day, and the depth of each turning is 1 / 3-1 / 2 of the total height of the material.

[0086] In the embodiment of the present application, the dried material is granulated, comprising: By adding a binder and controlling the speed and temperature of the granulator, a uniform granular product can be obtained; The granular product is screened and dried, and then the first layer of membrane solution is prepared and evenly impregnated on the surface of the granules to allow the organic solvent to evaporate and form a firm first layer of membrane; A second layer of film is prepared again, and the particles wrapped with the first layer of film are wrapped with the second layer of film, and then dried to obtain an ethoxybenzyldiethylenetriaminepentaacetic acid particle product wrapped with a double layer of film.

[0087] In the embodiment of the present application, the first layer membrane solution comprises the following components in parts by weight: Polyethylene: 65 parts; Cyclohexane: 45 parts; Zinc oxide: 4 parts; Citrate ester: 13 parts.

[0088] In the embodiment of the present application, the second layer membrane solution comprises the following components in parts by weight: Polylactic acid: 75 parts; Sodium alginate: 18 parts; Ferric chloride: 2.5 parts; Growth hormone: 2.5 parts.

[0089] Example 5 The invention provides a method for promoting microbial activity by using vitamin B12 fungus residue fertilizer. The method comprises the following steps: obtaining vitamin B12 fungus residue, pre-treating the vitamin B12 fungus residue, wherein the pre-treatment comprises drying the vitamin B12 fungus residue to reduce its moisture content to 10%-15%, and then pulverizing the vitamin B12 fungus residue to obtain uniform fungus residue powder; adding a modifying additive to the vitamin B12 fungus residue and stirring the mixture for 30-60 minutes to fully and evenly mix the mixture; inoculating fermentation bacteria into the mixed material, placing the inoculated material into a fermentation tank for fermentation, controlling the fermentation temperature to 30-35°C and the humidity to 60%-70% for 5-7 days; after the fermentation is completed, drying the material to reduce its moisture content to below 20%, and then granulating the dried material to prepare granular vitamin B12 fungus residue fertilizer; evenly spreading the vitamin B12 fungus residue fertilizer on the surface of field soil, and then plowing the soil to fully mix the fungus residue fertilizer with the soil, and applying the fungus residue fertilizer in a timely manner according to crop growth conditions and soil fertility conditions.

[0090] In the examples of the present application, the modifying additives are composed in the following weight ratios: Humic acid: 10 parts; Amino acids: 18 parts; Organic chelating agent: 6 parts; Cobalt sulfide: 3 parts; Microbial agent: 10 parts.

[0091] In the embodiment of the present application, the organic chelating agent is ethoxybenzyldiethylenetriaminepentaacetic acid.

[0092] In the examples of the present application, the preparation process of ethoxybenzyldiethylenetriaminepentaacetic acid is as follows: The pH value of diethylenetriamine is adjusted to the range of 5-10, and a two-step reaction is carried out with an aqueous solution of hydroxyacetonitrile at a molar ratio of 3.0:1 and 6:1 at a specific temperature to produce an intermediate product, diethylenetriamine pentaacetonitrile; converting the intermediate product into diethylenetriamine pentaacetic acid or a hydrolyzate of diethylenetriamine pentaacetic acid salt through a hydrolysis reaction, adding activated carbon to the hydrolyzate for decolorization and performing post-treatment to obtain solid diethylenetriamine pentaacetic acid; After obtaining diethylenetriamine pentaacetic acid, it reacts with a reagent containing ethoxybenzyl to form ethoxybenzyldiethylenetriamine pentaacetic acid.

[0093] In the embodiment of the present application, activated carbon is added to the hydrolyzate for decolorization and post-treatment, including: Determine whether the product is a salt or an acid. If it is a salt, convert it into diethylenetriaminepentaacetic acid by passing it through an ion exchange resin. Otherwise, remove the excess solvent by evaporation and concentration, then cool and crystallize, and collect the solid diethylenetriaminepentaacetic acid using a filtration device and dry it in an oven to constant weight.

[0094] In the embodiment of the present application, the microbial agent is a photosynthetic bacterial agent.

[0095] In the embodiment of the present application, the fermentation process also includes regular turning of the pile, wherein the turning frequency is 1-2 times a day, and the depth of each turning is 1 / 3-1 / 2 of the total height of the material.

[0096] In the embodiment of the present application, the dried material is granulated, comprising: By adding a binder and controlling the speed and temperature of the granulator, a uniform granular product can be obtained; The granular product is screened and dried, and then the first layer of membrane solution is prepared and evenly impregnated on the surface of the granules to allow the organic solvent to evaporate and form a firm first layer of membrane; A second layer of film is prepared again, and the particles wrapped with the first layer of film are wrapped with the second layer of film, and then dried to obtain an ethoxybenzyldiethylenetriaminepentaacetic acid particle product wrapped with a double layer of film.

[0097] In the embodiment of the present application, the first layer membrane solution comprises the following components in parts by weight: Polyethylene: 70 parts; Cyclohexane: 50 parts; Zinc oxide: 5 parts; Citrate ester: 15 parts.

[0098] In the embodiment of the present application, the second layer membrane solution comprises the following components in parts by weight: Polylactic acid: 80 parts; Sodium alginate: 20 parts; Ferric chloride: 3 parts; Auxin: 3 parts.

[0099] Comparative Example 1 A method for promoting microbial activity by using vitamin B12 fungus residue fertilizer is disclosed. The method differs from Example 1 only in that no organic chelating agent is added to the modified additive, and the rest of the preparation process is the same as that of Example 1.

[0100] Comparative Example 2 A method for promoting microbial activity by using vitamin B12 fungus residue fertilizer is provided. The method differs from Example 1 only in that a double-layer membrane structure is not prepared when the dried material is granulated, and the rest of the preparation process is the same as that of Example 1.

[0101] Comparative Example 3 A method for promoting microbial activity by using vitamin B12 fungus residue fertilizer is disclosed. The method differs from Example 1 only in that no organic chelating agent is added to the modified additive, and a double-layer membrane structure is not prepared when the dried material is granulated. The rest of the preparation process is the same as that of Example 1.

[0102] Performance Testing The vitamin B12 fungus residue fertilizers prepared in Examples 1-5 and Comparative Examples 1-3 were measured, and the results are shown in Table 1 below.

[0103] Table 1 Test results Soil microbial activity (CFU / g) Soil nutrient content (mg / kg) Crop yield (kg / mu) Fertilizer nutrient release cycle (days) Example 1 <![CDATA[5.2×10 6 ]]> 355 580 50 Example 2 <![CDATA[5.8×10 6 ]]> 376 610 55 Example 3 <![CDATA[6.5×10 6 ]]> 399 640 60 Example 4 <![CDATA[7.3×10 6 ]]> 424 690 65 Example 5 <![CDATA[6.9×10 6 ]]> 410 670 60 Comparative Example 1 <![CDATA[3.5×10 6 ]]> 248 480 45 Comparative Example 2 <![CDATA[4.2×10 6 ]]> 309 500 30 Comparative Example 3 <![CDATA[3.0×10 6 ]]> 210 450 25 The test results in Table 1 show that in terms of soil microbial activity, Examples 1 to 5 showed a significant upward trend, from 5.2×10 6 CFU / g gradually increased to 7.3×10 6 CFU / g decreased slightly to 6.9×10 6 CFU / g. This shows that with the adjustment of the proportion of each component in the modified additive, especially the reasonable increase of humic acid, amino acid, cobalt sulfide and microbial agents, the growth and reproduction of soil microorganisms have been actively promoted. However, the microbial activity of Comparative Examples 1 and 3 was significantly lower, at 3.5×10 6 CFU / g and 3.0×10 6 CFU / g, which highlights the key role of organic chelating agents in stimulating microbial activity, which may create a more favorable living environment for microorganisms by regulating the concentration and activity of metal ions in the soil, thereby promoting the growth and metabolism of microorganisms.

[0104] From the perspective of soil nutrient content, the values ​​in the examples are all higher than those in the comparative examples. In Example 1, the soil nutrient content is 355 mg / kg. As the examples progress, the nutrient content gradually increases, reaching 424 mg / kg in Example 4, and then slightly drops back to 410 mg / kg in Example 5. This is related to the changing trend of soil microbial activity. Higher microbial activity helps to accelerate the decomposition of organic matter and the conversion of nutrients in the soil, thereby increasing the content of nutrients such as nitrogen, phosphorus, and potassium in the soil. Comparative Examples 1 and 3 may have affected the activation and conversion process of nutrients in the soil by microorganisms due to the lack of organic chelating agents, resulting in relatively low soil nutrient content.

[0105] The crop yield data show that the yields of the embodiments are all higher than those of the comparative examples, and there is also an increasing trend between the embodiments as microbial activity and soil nutrient content increase. The crop yield of Example 1 is 580 kg / mu, which reaches 690 kg / mu in Example 4, and then slightly decreases to 670 kg / mu in Example 5. This fully demonstrates the effectiveness of the vitamin B12 fungus residue fertilizer in promoting crop growth and increasing yield. It provides more adequate nutrition for crops by improving the soil microbial environment and increasing soil nutrient supply, thereby promoting the growth and development of crops. The low yields of Comparative Examples 1 and 3 once again confirm the importance of organic chelating agents in fertilizer performance, and although the yield of Comparative Example 2 is slightly higher than that of Comparative Examples 1 and 3, the nutrient release cycle is shorter due to the lack of a double-layer membrane structure, and it may not be able to continuously provide sufficient nutrients in the later stage of crop growth, thereby affecting the final yield.

[0106] In terms of the fertilizer nutrient release cycle, Examples 1 to 5 showed a trend of gradual extension, from 50 days to 65 days, thanks to the rational formulation of the fertilizer and possible double-layer membrane wrapping processes. The double-layer membrane structure may have played a role in controlling the nutrient release rate, enabling it to provide a stable nutrient supply to crops over a longer period of time. In contrast, the nutrient release cycles of Comparative Examples 2 and 3 were significantly shorter, at 30 days and 25 days, respectively. This is because the double-layer membrane structure was not used or the organic chelating agent and double-layer membrane structure were missing at the same time, resulting in the rapid release of fertilizer nutrients in the soil, which could not meet the needs of the crop throughout its growth cycle and may also increase the risk of nutrient loss.

[0107] In summary, this application proposes a method for promoting microbial activity by using vitamin B12 fungus residue fertilizer, which optimizes the utilization of soil trace elements by finely synthesizing ethoxybenzyldiethylenetriaminepentaacetic acid as an organic chelating agent, and combines a variety of beneficial ingredients to jointly improve microbial activity and soil quality. At the same time, a double-layer membrane design is used to achieve precise slow-release control of fertilizer nutrients, reduce nutrient loss, and adapt to different soil environments. By using vitamin B12 fungus residue as the main raw material, waste resource utilization is realized, costs are reduced, the economic benefits of agricultural production are improved, and the use of pesticides and fertilizers is reduced. As a result, the problems of low component extraction and conversion efficiency, low microbial fermentation activity, and unstable product quality in the related technology are solved.

[0108] Although the embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A method for promoting microbial activity by using vitamin B12 bacterial residue fertilizer, characterized in that: Includes the following: Obtaining vitamin B12 bacterial residue, and pre-treating the vitamin B12 bacterial residue, wherein the pre-treatment includes drying the vitamin B12 bacterial residue to reduce its moisture content to 10%-15%, and then crushing the vitamin B12 bacterial residue to obtain a uniform bacterial residue powder; Adding a modified additive to the vitamin B12 bacterial residue and stirring for 30-60 minutes to fully mix the mixture; Inoculate the mixed material with fermentation bacteria, place the inoculated material into a fermentation tank for fermentation, control the fermentation temperature at 30-35°C, the humidity at 60%-70%, and the fermentation time for 5-7 days; After fermentation, the material is dried to reduce its moisture content to below 20%, and then the dried material is granulated to produce granular vitamin B12 fungus residue fertilizer; The vitamin B12 fungus residue fertilizer is evenly spread on the surface of the field soil, and then plowed to fully mix the fungus residue fertilizer with the soil. According to the crop growth and soil fertility, the fungus residue fertilizer can be topdressed in time.

2. The method for promoting microbial activity by using vitamin B12 bacterial residue fertilizer according to claim 1, wherein: The modified additive is composed of the following weight ratios: Humic acid: 5-10 parts; Amino acids: 10-18 parts; Organic chelating agent: 3-6 parts; Cobalt sulfide: 1-3 parts; Microbial agent: 5-10 parts.

3. The method for promoting microbial activity by using vitamin B12 bacterial residue fertilizer according to claim 1, wherein: The organic chelating agent is ethoxybenzyldiethylenetriaminepentaacetic acid.

4. The method for promoting microbial activity by using vitamin B12 bacterial residue fertilizer according to claim 3, wherein: The preparation process of the ethoxybenzyldiethylenetriaminepentaacetic acid is as follows: The pH value of diethylenetriamine is adjusted to a range of 5-10, and a two-step reaction is carried out with a hydroxyacetonitrile aqueous solution at a molar ratio of 1.5-3.0:1 and 2.5-6:1 at a specific temperature to generate an intermediate product, diethylenetriamine pentaacetonitrile; converting the intermediate product into diethylenetriamine pentaacetic acid or a hydrolyzate of diethylenetriamine pentaacetic acid salt through a hydrolysis reaction, adding activated carbon to the hydrolyzate for decolorization and performing post-treatment to obtain solid diethylenetriamine pentaacetic acid; After obtaining diethylenetriamine pentaacetic acid, it reacts with a reagent containing ethoxybenzyl to form ethoxybenzyldiethylenetriamine pentaacetic acid.

5. The method for promoting microbial activity by using vitamin B12 bacterial residue fertilizer according to claim 3, wherein: Activated carbon is added to the hydrolyzate for decolorization and post-treatment, including: Determine whether the product is a salt or an acid. If it is a salt, convert it into diethylenetriaminepentaacetic acid by passing it through an ion exchange resin. Otherwise, remove the excess solvent by evaporation and concentration, then cool and crystallize, and collect the solid diethylenetriaminepentaacetic acid using a filtration device and dry it in an oven to constant weight.

6. The method for promoting microbial activity by using vitamin B12 bacterial residue fertilizer according to claim 3, wherein: The microbial agent is a photosynthetic bacterial agent.

7. The method for promoting microbial activity by using vitamin B12 bacterial residue fertilizer according to claim 1, wherein: The fermentation process also includes regular turning of the pile, wherein the turning frequency is 1-2 times a day, and the depth of each turning is 1 / 3-1 / 2 of the total height of the material.

8. The method for promoting microbial activity by using vitamin B12 bacterial residue fertilizer according to claim 1, wherein: The dried material is granulated, including: By adding a binder and controlling the speed and temperature of the granulator, a uniform granular product can be obtained; The granular product is screened and dried, and then a first layer of membrane solution is prepared and evenly impregnated on the surface of the granules to volatilize the organic solvent and form a firm first layer of membrane; A second layer of film is prepared again, and the particles wrapped with the first layer of film are wrapped with the second layer of film, and then dried to obtain an ethoxybenzyldiethylenetriaminepentaacetic acid particle product wrapped with a double layer of film.

9. The method for promoting microbial activity by using vitamin B12 bacterial residue fertilizer according to claim 8, wherein: The first layer membrane solution is formulated according to the following weight ratios: Polyethylene: 50-70 parts; Cyclohexane: 30-50 parts; Zinc oxide: 1-5 parts; Citrate: 5-15 parts.

10. The method for promoting microbial activity by using vitamin B12 bacterial residue fertilizer according to claim 8, characterized in that: The second layer membrane solution is formulated according to the following weight ratios: Polylactic acid: 60-80 parts; Sodium alginate: 10-20 parts; Ferric chloride: 1-3 parts; Auxin: 0.1-3 parts.

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