Preparation method of amino acid-containing water-soluble fertilizer additive microbial agent

Abstract

The invention relates to the technical field of microbial agents, and discloses a preparation method of an amino acid-containing water-soluble fertilizer additive microbial agent, which comprises the following steps: S1, preparing raw materials, S2, preparing an amino acid solution, S3, adding nutrient elements, S4, pretreating the microbial agent, S5, adding and mixing auxiliary materials, S6, mixing the microbial agent, and S7, packaging and storing. According to the method, the activity of the microbial agents is remarkably improved, the microbial agents of bacillus subtilis, bacillus megaterium and bacillus mucilaginosus are massively propagated in a proper liquid culture medium environment through activation culture, the number of viable bacteria is remarkably increased, glycerol and trehalose protective agents are added, a protective film is formed, and the activity of the microbial agents is remarkably improved. According to the present invention, the damage of external adverse factors to the microbial agent can be effectively resisted, and the detection results show that the viable count of the microbial agent can be maintained at more than 80% of the initial value after the fertilizer product prepared by using the method is stored for 6 months so as to ensure the continuous nitrogen fixation, phosphorus solubilizing and potassium solubilizing effects in the soil, enhance the soil fertility and promote the crop growth.

Description

Technical Field

[0001] The invention relates to the technical field of microbial agents, in particular to a method for preparing a water-soluble fertilizer containing amino acids by adding a microbial agent. Background Art

[0002] In modern agricultural production, to meet the demand for high and high yields of crops, the scientific application of fertilizers has become a key link. The use of amino acid-containing water-soluble fertilizers and microbial agents alone or in combination is becoming increasingly common, but there are many challenges in practical application:

[0003] The activity of microbial agents is difficult to guarantee: The activity of microbial agents is crucial to their function. In traditional preparation methods, microbial agents are directly mixed with other ingredients. During mixing, storage, and subsequent fertilization, a large number of live bacteria are inactivated due to factors such as temperature, pH, and osmotic pressure. For example, under conventional mixing methods, the number of live bacteria in a microbial agent can drop by more than 50% after three months of storage, significantly reducing its nitrogen fixation, phosphorus solubilization, and potassium solubilization functions, making it ineffective in improving soil fertility and the crop growth environment.

[0004] Compatibility issues are prominent: Amino acid-containing water-soluble fertilizers have complex compositions. When mixed with microbial agents, the amino acids, macronutrients, and trace elements may interact with the agents. On the one hand, certain ions may affect the permeability of microbial cell membranes, inhibiting their growth. On the other hand, microbial agents may promote chemical reactions in some fertilizer components, leading to precipitation and stratification in the fertilizer solution, reducing fertilizer quality and effectiveness.

[0005] Low effectiveness of trace elements: Although trace elements are essential for crop growth, conventional addition methods often result in poor stability in fertilizer solutions. For example, ferrous sulfate is easily oxidized, reducing the effectiveness of the iron and making it difficult for crops to absorb and utilize it. Research indicates that the absorption rate of trace elements in traditional fertilizers is only 10%-20%, resulting in a waste of resources and failing to meet crop growth needs.

[0006] Extensive preparation technology: The existing preparation process lacks precise control over key process parameters such as temperature, pH value, and stirring speed. For example, mixing microbial agents and fertilizer ingredients in a high-temperature environment will accelerate the death of microorganisms. Stirring too fast or too slow will affect the uniform distribution of nutrients and the dispersion of microbial agents, resulting in uneven product quality and large differences between batches, making it difficult to ensure stable fertilization results. Summary of the Invention

[0007] (1) Technical problems solved

[0008] In response to the shortcomings of the existing technology, the present invention provides a method for preparing a water-soluble fertilizer containing amino acids by adding microbial agents, which has the advantages of significantly improving the activity of microbial agents and solves the problem of difficulty in ensuring the activity of microbial agents.

[0009] (2) Technical solution

[0010] To achieve the above-mentioned purpose of significantly improving the activity of microbial agents, the present invention provides the following technical solution: a method for preparing a water-soluble fertilizer containing amino acids and adding microbial agents, comprising S1 preparing raw materials, S2 preparing an amino acid solution, S3 adding nutrient elements, S4 pretreating the microbial agent, S5 adding and mixing auxiliary materials, S6 mixing the microbial agent, and S7 packaging and storage, wherein the preparation of the amino acid solution S2 includes S201 hydrolysis treatment and S202 filtration and neutralization;

[0011] Among them, the addition of S3 nutrients includes the dissolution of macroelements in S301 and the chelation of trace elements in S302;

[0012] Among them, the pretreatment of S4 microbial agent includes S401 activation culture and S402 addition of protective agent;

[0013] Among them, the addition and mixing of S5 auxiliary materials include the addition of S501 organic matter and the addition of S502 pH regulator and stabilizer.

[0014] Preferably, the S1 is prepared from raw materials:

[0015] Amino acid raw materials: Choose animal hair and plant proteins rich in multiple amino acids, such as soy protein hydrolysate, with an amino acid content of no less than 40%;

[0016] Nutrient elements: including nitrogen, phosphorus, and potassium, as well as trace elements such as calcium, magnesium, iron, and zinc. Urea (nitrogen content 46%), potassium dihydrogen phosphate (phosphorus content 52%, potassium content 34%), and magnesium sulfate (magnesium content 9.7%) are used.

[0017] Microbial agents: Use beneficial microorganisms with nitrogen fixation, phosphate solubilization, and potassium solubilization functions, such as Bacillus subtilis, Bacillus megaterium, and Bacillus mucilaginosus, with a viable bacterial count of no less than 200 million per gram;

[0018] Auxiliary materials: including humic acid, alginate organic matter, and appropriate amounts of pH regulators and stabilizers.

[0019] Preferably, the hydrolysis treatment in S201 is as follows: placing the soy protein hydrolyzate into a reactor, adding an appropriate amount of water, adjusting the solid-liquid ratio to 1:3, then adding a certain amount of acid (such as hydrochloric acid) as a catalyst, and performing a hydrolysis reaction at a temperature of 100-120° C. and a pressure of 0.1-0.2 MPa;

[0020] S202 Filtration and Neutralization: After the hydrolysis reaction is completed, the reaction solution is cooled to room temperature and then filtered using a filtration device to remove insoluble impurities. Subsequently, the pH value of the filtrate is adjusted to 6.5-7.5 using sodium hydroxide solution to make it neutral.

[0021] Preferably, the hydrolysis treatment in S201 has a reaction time of 2-3 hours, and the macromolecular protein is decomposed into small molecular amino acids through hydrolysis.

[0022] Preferably, the dissolution of the macronutrients in S301 is as follows: according to the formula requirements, appropriate amounts of urea and potassium dihydrogen phosphate are weighed and added to the amino acid solution in sequence, stirring while adding to fully dissolve the nutrients. The amount of urea added is controlled to be 10%-15% of the mass of the amino acid solution, and the amount of potassium dihydrogen phosphate added is controlled to be 5%-10%;

[0023] Chelation of trace elements in S302: Dissolve the trace elements in magnesium sulfate and ferrous sulfate in appropriate amounts of water, then add a chelating agent (such as EDTA) to carry out a chelation reaction. The reaction conditions are a temperature of 60-80°C.

[0024] Preferably, the reaction time of the chelation of trace elements in S302 is 1-2 hours, and the chelated trace element solution is slowly added to the amino acid solution containing the macroelements, and stirred evenly. The total amount of trace elements added is 2%-5% of the mass of the amino acid solution.

[0025] Preferably, the activation culture in S401 is as follows: inoculating Bacillus subtilis, Bacillus megaterium, and Bacillus mucilaginosus microbial agents into a liquid culture medium, and performing activation culture at a temperature of 30-35° C. and a rotation speed of 150-200 r / min;

[0026] S401 activation culture, the culture time is 12-24 hours, which further increases the number of live bacteria in the microbial inoculant and improves its activity;

[0027] S402 Addition of protective agent: Add an appropriate amount of protective agent, such as glycerol or trehalose, to the activated microbial agent solution. The amount of protective agent added is 5%-10% of the mass of the microbial agent solution. The protective agent can protect the activity of the microbial agent during the subsequent mixing process and reduce its influence by external factors.

[0028] Preferably, the addition of organic matter in step S501 is as follows: adding humic acid and alginic acid organic matter to the amino acid solution containing nutrient elements and stirring evenly. The amount of humic acid added is 3%-5% of the mass of the amino acid solution, and the amount of alginic acid added is 2%-3%. Organic matter can improve soil structure and enhance fertilizer efficiency.

[0029] S502 Addition of pH regulator and stabilizer: According to the pH value of the solution, add an appropriate amount of pH regulator (such as citric acid, sodium bicarbonate) to keep the pH value of the solution between 6.0-7.0. At the same time, add an appropriate amount of stabilizer (such as xanthan gum), the addition amount of which is 0.1%-0.3% of the solution mass. The stabilizer can improve the stability of the solution and prevent stratification and precipitation of the fertilizer during storage and use.

[0030] Preferably, the S6 microbial agent is mixed:

[0031] Slowly add the pretreated microbial agent solution to the amino acid solution containing auxiliary materials, stir and mix for 30-60 minutes at a temperature of 25-30°C and a rotation speed of 100-150r / min to evenly distribute the microbial agent in the fertilizer solution. Control the amount of microbial agent added so that the number of viable bacteria in the final fertilizer product is not less than 50 million / ml.

[0032] Preferably, the S7 packaging and storage:

[0033] The prepared water-soluble fertilizer containing amino acids and added with microbial agents is placed in a sealed container and labeled with the product name, ingredients, and instructions for use. The storage conditions should be a cool, dry place with a temperature controlled at 10-25°C, avoiding direct sunlight and high temperature environments to ensure the activity of the microbial agents and the quality of the fertilizer.

[0034] (3) Beneficial effects

[0035] Compared with the prior art, the present invention provides a method for preparing a water-soluble fertilizer containing amino acids by adding a microbial agent, which has the following beneficial effects:

[0036] 1. A method for preparing a water-soluble amino acid fertilizer by adding a microbial agent. The method significantly enhances the activity of the microbial agent: through activation culture, the microbial agents of Bacillus subtilis, Bacillus megaterium, and Bacillus mucilaginosus are multiplied in large quantities in a suitable liquid culture medium environment, and the number of live bacteria is significantly increased. At the same time, glycerol and trehalose protective agents are added to form a protective film, which effectively resists damage to the microbial agent from adverse external factors. After testing, the fertilizer product prepared by this method can still maintain the number of live bacteria of the microbial agent at more than 80% of the initial value after storage for 6 months, ensuring that it continues to play its role in nitrogen fixation, phosphorus solubilization, and potassium solubilization in the soil, enhancing soil fertility and promoting crop growth.

[0037] 2. The preparation method of the water-soluble amino acid fertilizer with added microbial agents optimizes the compatibility of the fertilizer: during the preparation process, by strictly controlling the order of addition of each component, reaction conditions, and adjusting the pH value, the adverse reactions between the water-soluble amino acid fertilizer and the microbial agent are effectively reduced. The microbial agent can be evenly and stably dispersed in the fertilizer solution. No precipitation or stratification occurs during storage and use of the fertilizer, thereby ensuring the consistency and stability of the fertilizer quality and improving the comprehensive use effect of the fertilizer.

[0038] 3. A method for preparing a water-soluble amino acid fertilizer with added microbial agents, which improves the utilization rate of trace elements: EDTA chelating agent is used to chelate trace elements in magnesium sulfate and ferrous sulfate to form stable chelates, effectively avoiding the oxidation and precipitation of trace elements in the fertilizer solution. The absorption rate of crops for chelated trace elements is increased to 40%-60%, greatly improving the effectiveness of trace elements, meeting the needs of crop growth for multiple nutrients, and helping to improve crop yield and quality.

[0039] 4. The preparation method of the water-soluble amino acid fertilizer with added microbial agents ensures stable product quality: the temperature, pH value, and stirring speed process parameters are precisely controlled during the preparation process. From the preparation of the amino acid solution to the mixing of the microbial agents, each link strictly adheres to the standards. This makes the quality of each batch of fertilizer products highly consistent, and the quality stability and reliability are greatly improved, providing stable and reliable fertilization guarantees for agricultural production and reducing agricultural losses caused by fertilizer quality problems. DETAILED DESCRIPTION

[0040] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0041] This solution provides a technical solution, specifically, a method for preparing a water-soluble fertilizer containing amino acids and adding a microbial agent, which specifically includes the following specific method steps: S1: preparing raw materials:

[0042] Amino acid raw materials: Choose animal hair and plant proteins rich in multiple amino acids, such as soy protein hydrolysate, with an amino acid content of no less than 40%;

[0043] Nutrient elements: including nitrogen, phosphorus, and potassium, as well as trace elements such as calcium, magnesium, iron, and zinc. Urea (nitrogen content 46%), potassium dihydrogen phosphate (phosphorus content 52%, potassium content 34%), and magnesium sulfate (magnesium content 9.7%) are used.

[0044] Microbial agents: Use beneficial microorganisms with nitrogen fixation, phosphate solubilization, and potassium solubilization functions, such as Bacillus subtilis, Bacillus megaterium, and Bacillus mucilaginosus, with a viable bacterial count of no less than 200 million per gram;

[0045] Excipients: including humic acid, alginic acid organic matter, and appropriate amount of pH regulator and stabilizer;

[0046] Preparation of S2 amino acid solution:

[0047] S201 hydrolysis treatment: Place the soy protein hydrolyzate in a reactor, add an appropriate amount of water, adjust the solid-liquid ratio to 1:3, then add a certain amount of acid (such as hydrochloric acid) as a catalyst, and carry out the hydrolysis reaction at a temperature of 100-120°C and a pressure of 0.1-0.2MPa;

[0048] S201 hydrolysis treatment, the reaction time is 2-3 hours, through hydrolysis, the large molecular protein is decomposed into small molecular amino acids;

[0049] S202 Filtration and Neutralization: After the hydrolysis reaction is completed, the reaction solution is cooled to room temperature and then filtered using a filtration device to remove insoluble impurities. Then, the pH value of the filtrate is adjusted to 6.5-7.5 with sodium hydroxide solution to make it neutral;

[0050] S3 nutritional elements added:

[0051] S301 Dissolution of macronutrients: According to the formula requirements, weigh appropriate amounts of urea and potassium dihydrogen phosphate macronutrients, and add them to the amino acid solution in sequence, stirring while adding to fully dissolve the nutrients. The amount of urea added is controlled to be 10%-15% of the mass of the amino acid solution, and the amount of potassium dihydrogen phosphate added is controlled to be 5%-10%;

[0052] Chelation of trace elements in S302: Dissolve the trace elements in magnesium sulfate and ferrous sulfate in appropriate amounts of water, then add a chelating agent (such as EDTA) to carry out a chelation reaction at a temperature of 60-80°C;

[0053] S302: Chelating of trace elements, reaction time 1-2 hours, slowly adding the chelated trace element solution to the amino acid solution containing the macroelements, continuing to stir evenly, the total amount of trace elements added is 2%-5% of the mass of the amino acid solution;

[0054] S4 microbial agent pretreatment:

[0055] S401 activation culture: Bacillus subtilis, Bacillus megaterium, and Bacillus mucilaginosus microbial agents are inoculated into a liquid culture medium and activated at a temperature of 30-35°C and a rotation speed of 150-200 r / min;

[0056] S401 activation culture, the culture time is 12-24 hours, which further increases the number of live bacteria in the microbial inoculant and improves its activity;

[0057] S402 Addition of protective agent: Add an appropriate amount of protective agent, such as glycerol or trehalose, to the activated microbial agent solution. The amount of protective agent added is 5%-10% of the mass of the microbial agent solution. The protective agent can protect the activity of the microbial agent during the subsequent mixing process and reduce its influence by external factors.

[0058] Addition and mixing of S5 excipients:

[0059] S501 Addition of organic matter: Add humic acid and alginic acid to the amino acid solution containing nutrient elements and stir evenly. The amount of humic acid added is 3%-5% of the amino acid solution mass, and the amount of alginic acid added is 2%-3%. Organic matter can improve soil structure and increase fertilizer efficiency.

[0060] S502 pH regulator and stabilizer addition: According to the pH value of the solution, add an appropriate amount of pH regulator (such as citric acid, sodium bicarbonate) to keep the pH value of the solution between 6.0-7.0. At the same time, add an appropriate amount of stabilizer (such as xanthan gum) at a rate of 0.1%-0.3% of the solution mass. The stabilizer can improve the stability of the solution and prevent stratification and precipitation of the fertilizer during storage and use.

[0061] Mixture of S6 microbial agents:

[0062] Slowly add the pretreated microbial agent solution to the amino acid solution containing auxiliary materials, stir and mix for 30-60 minutes at a temperature of 25-30°C and a rotation speed of 100-150r / min to evenly distribute the microbial agent in the fertilizer solution, and control the amount of microbial agent added so that the number of viable bacteria in the final fertilizer product is not less than 50 million / ml;

[0063] S7 packaging and storage:

[0064] Place the prepared amino acid water-soluble fertilizer with added microbial agents into a sealed container, affix a label with the product name, ingredients, and instructions, and store it in a cool, dry place at a temperature of 10-25°C, avoiding direct sunlight and high temperatures to ensure the activity of the microbial agents and the quality of the fertilizer.

[0065] Furthermore, the method significantly improves the activity of microbial agents: through activation culture, Bacillus subtilis, Bacillus megaterium, and Bacillus mucilaginosus microbial agents are multiplied in large quantities in a suitable liquid culture medium environment, and the number of live bacteria is significantly increased. At the same time, glycerol and trehalose protective agents are added to form a protective film, which effectively resists damage to the microbial agents by adverse external factors. After testing, the fertilizer product prepared by this method can still maintain the number of live bacteria of the microbial agent at more than 80% of the initial value after storage for 6 months, ensuring that it continues to play the role of nitrogen fixation, phosphorus solubilization, and potassium solubilization in the soil, enhancing soil fertility and promoting crop growth.

[0066] Furthermore, this method optimizes fertilizer compatibility: during the preparation process, by strictly controlling the order of addition of each component, reaction conditions and adjusting the pH value, the adverse reactions between the amino acid-containing water-soluble fertilizer and the microbial agent are effectively reduced, the microbial agent can be evenly and stably dispersed in the fertilizer solution, and there is no precipitation or stratification during storage and use of the fertilizer, which ensures the consistency and stability of the fertilizer quality and improves the comprehensive use effect of the fertilizer.

[0067] Furthermore, the method improves the utilization rate of trace elements: EDTA chelating agent is used to chelate trace elements such as magnesium sulfate and ferrous sulfate to form stable chelates, which effectively avoids the oxidation and precipitation problems of trace elements in the fertilizer solution. The absorption rate of crops for chelated trace elements is increased to 40%-60%, which greatly improves the effectiveness of trace elements, meets the needs of crop growth for multiple nutrients, and helps to improve crop yield and quality.

[0068] Furthermore, this method ensures stable product quality: the process parameters of temperature, pH value, and stirring speed are precisely controlled during the preparation process. From the preparation of amino acid solutions to the mixing of microbial agents, each link strictly follows the standards. This makes the quality of each batch of fertilizer products highly consistent, and the quality stability and reliability are greatly improved, providing stable and reliable fertilization guarantees for agricultural production and reducing agricultural losses caused by fertilizer quality problems.

[0069] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a water-soluble fertilizer containing amino acids and adding a microbial agent, comprising S1 preparing raw materials, S2 preparing an amino acid solution, S3 adding nutrients, S4 pretreating the microbial agent, S5 adding and mixing auxiliary materials, S6 mixing the microbial agent, and S7 packaging and storage, characterized in that: The preparation of the S2 amino acid solution includes S201 hydrolysis treatment and S202 filtration and neutralization; Among them, the addition of S3 nutrients includes the dissolution of macroelements in S301 and the chelation of trace elements in S302; Among them, the pretreatment of S4 microbial agent includes S401 activation culture and S402 addition of protective agent; Among them, the addition and mixing of S5 auxiliary materials include the addition of S501 organic matter and the addition of S502 pH regulator and stabilizer.

2. The method for preparing a water-soluble fertilizer containing amino acids and adding a microbial agent according to claim 1, wherein: Said S1 is prepared from raw materials: Amino acid raw materials: Choose animal hair and plant proteins rich in multiple amino acids, such as soy protein hydrolysate, with an amino acid content of no less than 40%; Nutrient elements: including nitrogen, phosphorus, and potassium, as well as trace elements such as calcium, magnesium, iron, and zinc. Urea (nitrogen content 46%), potassium dihydrogen phosphate (phosphorus content 52%, potassium content 34%), and magnesium sulfate (magnesium content 9.7%) are used. Microbial agents: Use beneficial microorganisms with nitrogen fixation, phosphate solubilization, and potassium solubilization functions, such as Bacillus subtilis, Bacillus megaterium, and Bacillus mucilaginosus, with a viable bacterial count of no less than 200 million per gram; Auxiliary materials: including humic acid, alginate organic matter, and appropriate amounts of pH regulators and stabilizers.

3. The method for preparing the water-soluble fertilizer containing amino acids and adding a microbial agent according to claim 1, wherein: The hydrolysis treatment in step S201 is as follows: placing the soy protein hydrolysate into a reactor, adding an appropriate amount of water, adjusting the solid-liquid ratio to 1:3, then adding a certain amount of acid (such as hydrochloric acid) as a catalyst, and performing a hydrolysis reaction at a temperature of 100-120° C. and a pressure of 0.1-0.2 MPa; S202 Filtration and Neutralization: After the hydrolysis reaction is completed, the reaction solution is cooled to room temperature and then filtered using a filtration device to remove insoluble impurities. Subsequently, the pH value of the filtrate is adjusted to 6.5-7.5 using sodium hydroxide solution to make it neutral.

4. The method for preparing a water-soluble fertilizer containing amino acids and adding a microbial agent according to claim 1, wherein: The S201 hydrolysis treatment has a reaction time of 2-3 hours, and the macromolecular protein is decomposed into small molecular amino acids through hydrolysis.

5. The method for preparing the water-soluble fertilizer containing amino acids and adding a microbial agent according to claim 1, wherein: Dissolution of macronutrients in step S301: According to the formula requirements, weigh appropriate amounts of urea and potassium dihydrogen phosphate macronutrients, and add them to the amino acid solution in sequence, stirring while adding to fully dissolve the nutrients. The amount of urea added is controlled to be 10%-15% of the mass of the amino acid solution, and the amount of potassium dihydrogen phosphate added is controlled to be 5%-10%; Chelation of trace elements in S302: Dissolve the trace elements in magnesium sulfate and ferrous sulfate in appropriate amounts of water, then add a chelating agent (such as EDTA) to carry out a chelation reaction. The reaction conditions are a temperature of 60-80°C.

6. The method for preparing a water-soluble fertilizer containing amino acids and adding a microbial agent according to claim 1, wherein: The reaction time of the chelation of trace elements in S302 is 1-2 hours. The chelated trace element solution is slowly added to the amino acid solution containing the macroelements and stirred evenly. The total amount of trace elements added is 2%-5% of the mass of the amino acid solution.

7. The method for preparing a water-soluble fertilizer containing amino acids and adding a microbial agent according to claim 1, wherein: The S401 activation culture: inoculating Bacillus subtilis, Bacillus megaterium, and Bacillus mucilaginosus microbial agents into a liquid culture medium, and performing activation culture at a temperature of 30-35° C. and a rotation speed of 150-200 r / min; S401 activation culture, the culture time is 12-24 hours, which further increases the number of live bacteria in the microbial inoculant and improves its activity; S402 Addition of protective agent: Add an appropriate amount of protective agent, such as glycerol or trehalose, to the activated microbial agent solution. The amount of protective agent added is 5%-10% of the mass of the microbial agent solution. The protective agent can protect the activity of the microbial agent during the subsequent mixing process and reduce its influence by external factors.

8. The method for preparing a water-soluble fertilizer containing amino acids and adding a microbial agent according to claim 1, wherein: The addition of organic matter in step S501: adding humic acid and alginic acid to the amino acid solution containing nutrient elements and stirring evenly. The amount of humic acid added is 3%-5% of the mass of the amino acid solution, and the amount of alginic acid added is 2%-3%. The organic matter can improve the soil structure and increase the fertilizer efficiency. S502 Addition of pH regulator and stabilizer: According to the pH value of the solution, add an appropriate amount of pH regulator (such as citric acid, sodium bicarbonate) to keep the pH value of the solution between 6.0-7.

0. At the same time, add an appropriate amount of stabilizer (such as xanthan gum), the addition amount of which is 0.1%-0.3% of the solution mass. The stabilizer can improve the stability of the solution and prevent stratification and precipitation of the fertilizer during storage and use.

9. The method for preparing a water-soluble fertilizer containing amino acids and adding a microbial agent according to claim 1, wherein: Mixing of the S6 microbial inoculant: Slowly add the pretreated microbial agent solution to the amino acid solution containing auxiliary materials, stir and mix for 30-60 minutes at a temperature of 25-30°C and a rotation speed of 100-150r / min to evenly distribute the microbial agent in the fertilizer solution. Control the amount of microbial agent added so that the number of viable bacteria in the final fertilizer product is not less than 50 million / ml.

10. The method for preparing a water-soluble fertilizer containing amino acids and adding a microbial agent according to claim 1, characterized in that: The S7 packaging and storage: The prepared water-soluble fertilizer containing amino acids and added with microbial agents is placed in a sealed container and labeled with the product name, ingredients, and instructions for use. The storage conditions should be a cool, dry place with a temperature controlled at 10-25°C, avoiding direct sunlight and high temperature environments to ensure the activity of the microbial agents and the quality of the fertilizer.