Modularly assembled cell culture device and use method thereof

Abstract

The invention discloses a modularly-assembled cell culture device and a use method thereof, and belongs to the technical field of cell culture, culture spaces of culture vessels of different specifications are met by adjusting the distance between two adjacent supporting plates, pull rods are clamped into clamping grooves to lock the positions of the supporting plates, and the supporting plates are clamped into the clamping grooves. The requirement for adjusting culture spaces of culture vessels of different specifications is further met, the two supporting bearings are driven by the culture frame to rotate outside the fixing column, the culture module containing the culture vessels moves to one side in the culture box and is blocked by the culture box, and the different culture vessels are placed in different culture cavities respectively, so that the culture space of the culture vessels of different specifications is adjusted. According to the cell culture box, the culture space can be adaptively adjusted according to the growth scale of cells, and a plurality of culture cavities are integrated in the single culture box and can rotate, so that culture vessels are convenient to take and place, co-culture of different types of cells is realized, and multiple types of culture vessels do not need to be additionally arranged for use.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and in particular to a modularly assembled cell culture device and a method for using the same. Background Art

[0002] In modern biomedical research, cellular-level studies are becoming increasingly complex, from the exploration of basic cellular physiological functions to the study of disease mechanisms, such as the analysis of the proliferation, differentiation, and metastasis mechanisms of tumor cells, all require precise control of the cell culture environment.

[0003] Existing cell culture devices are usually of an integrated design, which lacks flexibility when used to meet the needs of culturing different types of cells. The internal structure and function of the device are relatively fixed. It is difficult for an integrated cell culture device to achieve the co-cultivation of certain special cell types or multiple cells. At the same time, it is not convenient to adaptively adjust the culture space according to the scale of cell growth. Multiple types of culture containers need to be added for use, which is inconvenient to operate and not easy to carry.

[0004] Based on this, the present invention designs a modular assembled cell culture device and a method of using the same to solve the above problems. Summary of the Invention

[0005] The object of the present invention is to provide a modularly assembled cell culture device and a method for using the same, so as to solve the problem raised in the above background technology that the existing cell culture devices are usually of one-piece design, lack flexibility in use when facing the needs of culturing different types of cells, and the internal structure and function of the device are relatively fixed. The one-piece cell culture device is difficult to achieve the co-cultivation of certain special cell types or multiple cells. At the same time, it is not convenient to adaptively adjust the culture space according to the scale of cell growth, and multiple types of culture containers need to be added for use.

[0006] To achieve the above object, the present invention provides the following technical solutions: A modularly assembled cell culture device includes an incubator, wherein a fixed column is fixedly provided through the middle of the incubator, and two support bearings are sleeved on the outer wall of the fixed column. A culture rack is provided inside the incubator, and the two support bearings are clamped above and below the middle of the culture rack. Four culture chambers are defined on the outside of the culture rack, and a plurality of culture modules are slidably mounted in the culture chambers. A plurality of culture vessels are mounted on the culture modules. Two sliding bars are fixed to both sides of the inner wall of the culture chamber, and the two sides of the culture modules are slidably connected to the outside of two opposing sliding bars. A locking assembly is fixedly connected to the bottom of the culture module. A plurality of slots are defined on the inner wall of the culture chamber near the fixed column, and an end of the locking assembly is clamped in one of the slots. A conduction assembly is fixedly installed on the top of the fixed column, and the top of the conduction assembly passes through the top of the incubator and is connected to a constant temperature connection pipe. The four culture chambers are connected to four ports outside the conduction assembly. A drive assembly is fixedly installed at the bottom of the incubator, and discharge valve pipes are respectively connected to the positions of the four culture chambers at the bottom of the culture rack.

[0007] As a further solution of the present invention, the bottom end of the fixed column is fixedly connected to a base, one side of the base is fixed to the bottom of the drive assembly, a sealed door is installed on the outside of the incubator through a hinge, and a display module and a control panel are installed on the side of the outer wall of the incubator corresponding to the sealed door.

[0008] As a further solution of the present invention, the culture module includes a support plate, which is slidably connected to the inside of the culture chamber, and a number of anti-slip pads are fixed on the support plate. A number of baffles are equidistantly arranged around the support plate corresponding to the anti-slip pads. The bottom of the culture vessel overlaps with the anti-slip pad, and the culture vessel is located between the number of baffles. A number of through holes are provided on the support plate, and two slide grooves are provided on both sides of the support plate, and the slide bars are slidably connected in the slide grooves.

[0009] As a further solution of the present invention, the locking assembly includes a pull rod, one end of the pull rod is fixedly connected to a handle, and the other end of the pull rod is clamped in a slot, a sliding sleeve is connected to the outer outer surface of the pull rod, a baffle is fixed to the outer wall of the sliding sleeve, and the baffle is fixed to the bottom of the support plate, and a spring is connected to the outer outer surface of the pull rod, and the two ends of the spring are respectively fixed to one side of the bottom of the support plate and the outside of the pull rod.

[0010] As a further solution of the present invention, the conduction component includes a bearing seat, which is clamped on the top of the inner wall of the incubator. A swivel is sleeved inside the bearing seat. Four connecting tubes are fixed through the swivel, and the bottom of the swivel is fixed to the top of the culture rack. One end of the connecting tube passes through the top of the culture rack and is connected to the culture chamber, and the other ends of the four connecting tubes are connected to a rotating joint.

[0011] As a further solution of the present invention, the fixed end of the bottom of the rotary joint is clamped on the top of the fixed column, and the movable end of the top of the rotary joint is connected to the bottom end of the constant temperature connecting tube and a sleeve is fixed on the outer wall. The sleeve passes through and rotates on the top of the incubator. A solenoid valve is installed on the outer wall of the connecting tube near the rotary joint. A wire is connected to the solenoid valve. The wire passes through the sleeve and the incubator and extends to the top of the incubator.

[0012] As a further solution of the present invention, the drive assembly includes a limit bearing, which is clamped on the bottom of the incubator, and a clamping ring is sleeved inside the limit bearing. The top of the clamping ring is fixed to the bottom of the incubator rack, and a gear ring is fixed to the bottom of the clamping ring. A gear is meshed with the outside of the gear ring, and a motor is fixed to the bottom of the gear. The fixing column passes through the middle of the clamping ring and the gear ring, and the discharge valve pipe passes through and is fixed in the clamping ring.

[0013] A method for using a modularly assembled cell culture device, comprising the following steps: When culturing cells, the sealed door is opened to expose the culture rack and the culture chamber to the outside, and the multiple culture modules in the culture chamber are facing the opening of the incubator. The culture vessels containing the cells are placed on the anti-slip mats in the culture modules. The anti-slip effect of the culture vessels is improved by adding anti-slip mats. A number of blocking bars are evenly spaced around the corresponding anti-slip mats on the support plate. The outer sides of the culture vessels are blocked and limited by the blocking bars, thereby preventing the culture vessels from shifting and colliding with each other. When the cam is in a fixed position, the cam is released, and the lever is moved forwards to move the lever so that the cam is in a fixed position and the lever is moved out of the position of the cam, thereby preventing the cam from being dislodged from the cam and from falling out of the cam. When adjusting the positions of the culture modules in the other three culture chambers, the control panel is operated outside the incubator to control the motor to work. The output shaft of the motor drives the gear to rotate, and the gear drives the clamping ring to rotate inside the limit bearing through the gear ring. The clamping ring drives the culture rack above to rotate, and the culture rack drives the two support bearings to rotate outside the fixed column. The clamping ring is supported by the limit bearing, and the culture rack is supported by the support bearing, thereby improving the stability of the rotation of the culture rack driven by the clamping ring. During this process, the culture module containing the culture vessel is moved to one side of the incubator and blocked by the incubator to prevent it from being exposed to the outside. After the culture rack rotates 90 degrees, the other culture chamber is moved to the opening on the front of the incubator. Similarly, the position of the culture module in the current culture chamber is adjusted. In this way, the four culture chambers are independently designed. By placing different culture vessels in different culture chambers, and integrating multiple culture chambers into a single incubator, after the culture vessels are placed, the sealing door is closed to seal the opening on the front of the incubator, so that different types of cells can be cultured together. During the culture process, the constant temperature connection tube is connected to the external constant temperature equipment, and the medium is introduced into the rotary joint through the constant temperature tube. The movable end at the top of the rotary joint is connected to the constant temperature connection tube. When the culture rack drives the swivel to rotate inside the bearing seat, the swivel drives the movable end at the top of the rotary joint to rotate through the four connecting tubes without affecting the normal conduction between the constant temperature connection tube and the rotary joint. During this process, the culture rack, swivel, connecting tube and the ferrule on the rotary joint rotate synchronously, so that the connecting tube will input the medium into the culture chamber connected to it. By controlling the solenoid valve outside the connecting tube, the environment in different culture chambers can be independently adjusted. The wire at the top of the solenoid valve passes through the ferrule and extends to the outside of the incubator to energize the solenoid valve. After entering the culture chamber, the medium will be introduced into the lower layer through the through hole on the support plate, thereby improving the constant temperature effect inside the culture chamber. After opening the discharge valve tube at the bottom of the culture rack, the medium inside the culture chamber will be discharged, thereby improving the fluidity of the medium inside the culture chamber.

[0014] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention adjusts the spacing between two adjacent support plates to meet the culture space requirements of culture vessels of different specifications. By pulling the handle at the bottom of the support plate, the end of the pull rod will disengage from the slot in the culture chamber, and the support plate is adjusted up and down to slide freely in the culture chamber. After adjusting the support plate to a suitable position, the handle is released, and the pull rod is supported by the elastic force of the spring, so that the pull rod slides outside the sliding sleeve, and the sliding sleeve limits the pull rod to improve the stability of the horizontal movement of the pull rod until the end of the pull rod is stuck in the slot, thereby achieving the purpose of locking the support plate. At this time, the spacing between the two adjacent support plates changes. If the position of the other support plates is continued to be adjusted, the operation is performed in the same way, thereby meeting the need to adjust the culture space of culture vessels of different specifications. When adjusting the positions of the culture modules in the other three culture chambers, the culture rack drives the two support plates to move. The bearing rotates outside the fixed column, and the culture module containing the culture vessel moves to one side of the incubator and is blocked by the incubator to prevent it from being exposed to the outside. After the culture rack rotates 90 degrees, the other culture chamber moves to the opening on the front of the incubator. Similarly, the position of the culture module in the current culture chamber is adjusted. In this way, and the four culture chambers are all independently designed, by placing different culture vessels in different culture chambers, the culture space can be adaptively adjusted according to the scale of cell growth. Multiple culture chambers are integrated in a single incubator and can be rotated, which is convenient for taking and placing culture vessels. After the culture vessels are placed, the sealing door is closed to seal the opening on the front of the incubator, so that different types of cells can be cultured together without the need to use multiple types of culture containers. 2. The present invention connects the constant temperature connection tube to an external constant temperature device, and the medium is introduced into the rotary joint through the constant temperature tube. The movable end at the top of the rotary joint is connected to the constant temperature connection tube. When the culture rack drives the rotating ring to rotate inside the bearing seat, the rotating ring drives the movable end at the top of the rotary joint to rotate through the four connecting tubes without affecting the normal conduction between the constant temperature connection tube and the rotary joint. During this process, the culture rack, the rotating ring, the connecting tube and the ferrule on the rotary joint rotate synchronously, so that the connecting tube will input the medium into the culture chamber connected thereto. By controlling the solenoid valve outside the connecting tube, the environment in different culture chambers can be independently adjusted. After entering the culture chamber, the medium will be introduced into the lower layer through the through hole on the support plate, thereby improving the constant temperature effect inside the culture chamber. During the rotation of the culture rack, the discharge valve tube will rotate synchronously. After opening the discharge valve tube at the bottom of the culture rack, the medium inside the culture chamber will be discharged, thereby improving the fluidity of the medium inside the culture chamber and facilitating the individual adjustment of the internal environment of multiple culture chambers. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0016] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention; Figure 2 This is a schematic diagram of the structure of the incubator of the present invention from the front; Figure 3 It is a structural schematic diagram of the cross section of the incubator of the present invention; Figure 4 It is a structural schematic diagram of the conducting component of the present invention; Figure 5 It is a structural schematic diagram of a partial cross section of the culture rack of the present invention; Figure 6 This is a schematic structural diagram of the culture rack of the present invention when viewed from above; Figure 7 Schematic diagram of the structure of the culture module of the present invention; Figure 8 It is a structural schematic diagram of the locking assembly of the present invention; Figure 9 Schematic diagram of the structure of the drive assembly of the present invention.

[0017] In the accompanying drawings, the components represented by the reference numerals are as follows: 1. Incubator; 2. Fixed column; 3. Support bearing; 4. Incubation rack; 5. Incubation chamber; 6. Incubation module; 601. Support plate; 602. Anti-slip pad; 603. Baffle; 604. Through hole; 605. Slide; 7. Incubation vessel; 8. Slide; 9. Slot; 10. Locking assembly; 101. Pull rod; 102. Handle; 103. Slide sleeve; 104. Baffle; 105. Spring; 11. Conductive assembly; 1 11. Bearing seat; 112. Swivel; 113. Rotary joint; 114. Ferrule; 115. Connecting pipe; 116. Solenoid valve; 117. Wire; 12. Constant temperature connecting pipe; 13. Drive assembly; 131. Limit bearing; 132. Snap ring; 133. Ring gear; 134. Gear; 135. Motor; 14. Discharge valve pipe; 15. Base; 16. Sealing door; 17. Display module; 18. Control panel. DETAILED DESCRIPTION

[0018] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0019] See also Figures 1-9 , the present invention provides a technical solution: A modular assembled cell culture device includes an incubator 1, a fixed column 2 is fixed through the middle of the incubator 1, and two support bearings 3 are sleeved on the outer wall of the fixed column 2. A culture rack 4 is provided inside the incubator 1, and two support bearings 3 are clamped above and below the middle of the culture rack 4. Four culture chambers 5 are opened outside the culture rack 4, and a number of culture modules 6 are slidably installed in the culture chamber 5. A number of culture vessels 7 are mounted on the culture modules 6. Two slide bars 8 are fixed on both sides of the inner wall of the culture chamber 5, and the two sides of the culture module 6 are slidably connected to the outside of the two opposite slide bars 8. The bottom of the culture module 6 is fixedly connected to a locking assembly 10. Several slots 9 are opened on the inner wall of the culture chamber 5 near the fixed column 2. The end of the locking assembly 10 is snapped into one of the slots 9. The top of the fixed column 2 is fixedly installed with a conducting assembly 11. The top of the conducting assembly 11 passes through the top of the incubator 1 and is connected to a constant temperature connecting pipe 12. The four culture chambers 5 are connected to the four ports outside the conducting assembly 11. The bottom of the incubator 1 is fixedly installed with a driving assembly 13. The bottom of the culture rack 4 is connected to the positions of the four culture chambers 5 respectively. The discharge valve pipe 14 is installed; The four culture chambers 5 are all independently designed. By placing different culture vessels 7 in different culture chambers 5 respectively, and integrating multiple culture chambers 5 inside a single incubator 1, different types of cells can be cultured together.

[0020] As a further embodiment of the present invention, the bottom end of the fixed column 2 is fixedly connected to a base 15, one side of the base 15 is fixed to the bottom of the drive assembly 13, a sealed door 16 is hingedly mounted on the outside of the incubator 1, and a display module 17 and a control panel 18 are mounted on the side of the outer wall of the incubator 1 corresponding to the sealed door 16; During operation, after the culture vessel 7 is placed, the sealing door 16 is closed to seal the opening on the front of the incubator 1 . The display module 17 is used to display the internal environmental data of the incubator 1 so as to grasp the culture status in real time.

[0021] As a further embodiment of the present invention, the culture module 6 includes a support plate 601, which is slidably connected to the interior of the culture chamber 5. A plurality of anti-slip pads 602 are fixed to the support plate 601. A plurality of blocking bars 603 are equidistantly arranged around the support plate 601 corresponding to the anti-slip pads 602. The bottom of the culture vessel 7 overlaps the anti-slip pad 602, and the culture vessel 7 is located between the plurality of blocking bars 603. The support plate 601 is provided with a plurality of through holes 604, and two slide grooves 605 are respectively provided on both sides of the support plate 601. The slide bar 8 is slidably connected in the slide groove 605. The support plate 601 is adjusted up and down so that it can slide freely in the culture chamber 5. At the same time, the slide grooves 605 on both sides of the support plate 601 slide outside the slide bar 8, playing a role in supporting and limiting the support plate 601, preventing the support plate 601 from shifting its position during movement and preventing the support plate 601 from leaving the culture chamber 5, thereby improving the stability of the adjustment of the support plate 601. During operation, the culture vessel 7 containing cells is placed on the anti-slip pad 602 in the culture module 6. The anti-slip effect of the culture vessel 7 is improved by adding the anti-slip pad 602, and a number of blocking bars 603 are equidistantly arranged around the corresponding anti-slip pad 602 on the support plate 601. The outer side of the culture vessel 7 is blocked and limited by the several blocking bars 603, thereby preventing the culture vessel 7 from deviating and colliding with each other.

[0022] As a further solution of the present invention, the locking assembly 10 includes a pull rod 101, one end of which is fixedly connected to a handle 102, and the other end of the pull rod 101 is clamped in the clamping slot 9, the pull rod 101 is outer-connected with a sliding sleeve 103, the outer wall of the sliding sleeve 103 is fixed with a baffle 104, the baffle 104 is fixed to the bottom of the support plate 601, the pull rod 101 is outer-connected with a spring 105, and the two ends of the spring 105 are respectively fixed to one side of the bottom of the support plate 601 and the outer side of the pull rod 101; During operation, the pull rod 101 is supported by the elastic force of the spring 105, so that the pull rod 101 slides outside the sliding sleeve 103, and the sliding sleeve 103 limits the pull rod 101 to improve the stability of the horizontal movement of the pull rod 101 until the end of the pull rod 101 is stuck in the slot 9, which facilitates locking the position of the support plate 601.

[0023] As a further embodiment of the present invention, the conduction assembly 11 includes a bearing seat 111, which is clamped to the top of the inner wall of the incubator 1. A swivel 112 is sleeved inside the bearing seat 111. Four connecting tubes 115 are fixedly inserted into the swivel 112. The bottom of the swivel 112 is fixed to the top of the culture rack 4. One end of the connecting tube 115 passes through the top of the culture rack 4 and communicates with the culture chamber 5. The other ends of the four connecting tubes 115 are connected to a rotary joint 113. During operation, the medium is introduced into the rotary joint 113 through the constant temperature tube, and the movable end at the top of the rotary joint 113 is connected to the constant temperature connection tube 12. When the culture rack 4 drives the rotating ring 112 to rotate inside the bearing seat 111, the rotating ring 112 drives the movable end at the top of the rotary joint 113 to rotate through the four connecting tubes 115 without affecting the normal conduction between the constant temperature connection tube 12 and the rotary joint 113.

[0024] As a further embodiment of the present invention, the fixed end of the bottom of the rotary joint 113 is clamped on the top of the fixed column 2, and the movable end of the top of the rotary joint 113 is connected to the bottom end of the constant temperature connection tube 12. A sleeve 114 is fixed to the outer wall of the rotary joint 113. The sleeve 114 passes through and rotates on the top of the incubator 1. A solenoid valve 116 is installed on the outer wall of the connecting tube 115 near the rotary joint 113. A wire 117 is connected to the solenoid valve 116. The wire 117 passes through the sleeve 114 and the incubator 1 and extends to the top of the incubator 1. During operation, the connecting tube 115 will input the medium into the culture chamber 5 connected to it, and by controlling the solenoid valve 116 outside the connecting tube 115, the environment in different culture chambers 5 can be independently adjusted. The wire 117 on the top of the solenoid valve 116 passes through the sleeve 114 and extends to the outside of the incubator 1 to energize the solenoid valve 116. After the medium enters the culture chamber 5, it will be introduced into the lower layer through the through hole 604 on the support plate 601, thereby improving the constant temperature effect inside the culture chamber 5.

[0025] As a further embodiment of the present invention, the drive assembly 13 includes a limit bearing 131, which is clamped to the bottom of the incubator 1. A retaining ring 132 is sleeved inside the limit bearing 131. The top of the retaining ring 132 is fixed to the bottom of the culture rack 4. A gear ring 133 is fixed to the bottom of the retaining ring 132. The gear ring 133 is externally meshed with a gear 134. A motor 135 is fixed to the bottom of the gear 134. The fixing column 2 passes through and is sleeved in the middle of the retaining ring 132 and the gear ring 133. During operation, the output shaft of the motor 135 drives the gear 134 to rotate, and the gear 134 drives the retaining ring 132 to rotate inside the limiting bearing 131 through the ring gear 133. The retaining ring 132 drives the culture rack 4 above to rotate, and the culture rack 4 drives the two support bearings 3 to rotate outside the fixed column 2. The retaining ring 132 is supported by the limiting bearing 131, and the culture rack 4 is supported by the support bearing 3, thereby improving the stability of the rotation of the culture rack 4 driven by the retaining ring 132.

[0026] The discharge valve tube 14 is passed through and fixed in the clamp ring 132 . After the discharge valve tube 14 at the bottom of the culture rack 4 is opened, the medium inside the culture chamber 5 is discharged, thereby improving the fluidity of the medium inside the culture chamber 5 .

[0027] A method for using a modularly assembled cell culture device, comprising the following steps: When culturing cells, the sealed door 16 is opened to expose the culture rack 4 and the culture chamber 5 to the outside, and the multiple culture modules 6 in the culture chamber 5 are facing the opening of the incubator 1. The culture vessels 7 containing the cells are placed on the anti-slip mats 602 in the culture modules 6. The anti-slip effect of the culture vessels 7 is improved by adding the anti-slip mats 602. A plurality of blocking bars 603 are evenly spaced around the supporting plate 601 corresponding to the anti-slip mats 602. The blocking bars 603 block the outer sides of the culture vessels 7, thereby preventing the culture vessels 7 from deviating and colliding with each other. When adjusting the spacing between two adjacent support plates 601 to meet the culture space requirements of culture vessels 7 of different specifications, by pulling the handle 102 at the bottom of the support plate 601, the handle 102 drives the pull rod 101 to move forward, so that the end of the pull rod 101 will be out of the slot 9 in the culture chamber 5. At this time, the support plate 601 is in a free movable state in the culture chamber 5. The support plate 601 is adjusted up and down to slide freely in the culture chamber 5. At the same time, the slide grooves 605 on both sides of the support plate 601 slide outside the slide bar 8, which plays a role in supporting and limiting the support plate 601, preventing the support plate 601 from shifting position during movement and the support plate 601 will not be out of the culture chamber 5, thereby improving To ensure the stability of the support plate 601, after adjusting the support plate 601 to a suitable position, release the handle 102, and support the pull rod 101 with the elastic force of the spring 105, so that the pull rod 101 slides outside the sliding sleeve 103, and the sliding sleeve 103 limits the pull rod 101, thereby improving the stability of the horizontal movement of the pull rod 101, until the end of the pull rod 101 is stuck in the slot 9, thereby achieving the purpose of locking the support plate 601. At this time, the distance between the two adjacent support plates 601 changes. If the position of the other support plates 601 is continued to be adjusted, the operation is performed in the same way, thereby meeting the demand for adjusting the culture space of culture vessels 7 of different specifications; When adjusting the positions of the culture modules 6 in the other three culture chambers 5, the control panel 18 is operated outside the incubator 1 to control the motor 135 to work. The output shaft of the motor 135 drives the gear 134 to rotate. The gear 134 drives the snap ring 132 to rotate inside the limit bearing 131 through the gear ring 133. The snap ring 132 drives the culture rack 4 above to rotate. The culture rack 4 drives the two support bearings 3 to rotate outside the fixed column 2. The snap ring 132 is supported by the limit bearing 131, and the support bearing 3 supports the culture rack 4, thereby improving the stability of the culture rack 4 driven by the snap ring 132 to rotate. In this process, the culture rack 4 equipped with The culture module 6 of the vessel 7 is moved to one side of the incubator 1 and is blocked by the incubator 1 so that it is not exposed to the outside. After the culture rack 4 is rotated 90 degrees, another culture chamber 5 is moved to the opening on the front of the incubator 1. Similarly, the position of the culture module 6 in the current culture chamber 5 is adjusted. In this way, the four culture chambers 5 are all independently designed. By placing different culture vessels 7 in different culture chambers 5, and integrating multiple culture chambers 5 in a single incubator 1, after the culture vessels 7 are placed, the sealing door 16 is closed to seal the opening on the front of the incubator 1, so that different types of cells can be cultured together. During the culture process, the constant temperature connection tube 12 is connected to the external constant temperature equipment, and the medium is introduced into the rotary joint 113 through the constant temperature tube. The movable end at the top of the rotary joint 113 is connected to the constant temperature connection tube 12. When the culture rack 4 drives the rotating ring 112 to rotate inside the bearing seat 111, the rotating ring 112 drives the movable end at the top of the rotary joint 113 to rotate through the four connecting tubes 115 without affecting the normal conduction between the constant temperature connection tube 12 and the rotary joint 113. During this process, the culture rack 4, the rotating ring 112, the connecting tube 115 and the ferrule 114 on the rotary joint 113 rotate synchronously, so that the connection The tube 115 will input the medium into the culture chamber 5 connected to it. By controlling the solenoid valve 116 outside the connecting tube 115, the environment in different culture chambers 5 can be independently adjusted. The wire 117 on the top of the solenoid valve 116 passes through the sleeve 114 and extends to the outside of the incubator 1 to energize the solenoid valve 116. After the medium enters the culture chamber 5, it will be introduced into the lower layer through the through hole 604 on the support plate 601, thereby improving the constant temperature effect inside the culture chamber 5. After opening the discharge valve tube 14 at the bottom of the culture rack 4, the medium inside the culture chamber 5 will be discharged, thereby improving the fluidity of the medium inside the culture chamber 5.

Claims

1. A modular cell culture device comprising an incubator (1), characterized in that: A fixed column (2) is fixed through the middle of the incubator (1), and two support bearings (3) are sleeved on the outer wall of the fixed column (2). A culture rack (4) is provided inside the incubator (1), and two support bearings (3) are clamped at the upper and lower parts of the middle of the culture rack (4). Four culture chambers (5) are provided outside the culture rack (4), and a plurality of culture modules (6) are slidably mounted in the culture chambers (5). A plurality of culture vessels (7) are mounted on the culture modules (6). Two slide bars (8) are fixed on both sides of the inner wall of the culture chamber (5), and the two sides of the culture module (6) are slidably connected to the outside of the two opposite slide bars (8). The bottom of the culture module (6) is provided with a plurality of culture chambers (5). A locking assembly (10) is fixedly connected, and a plurality of slots (9) are provided on the inner wall of the culture chamber (5) near the fixed column (2), and the end of the locking assembly (10) is clamped in one of the slots (9). A conducting assembly (11) is fixedly installed on the top of the fixed column (2), and the top of the conducting assembly (11) passes through the top of the incubator (1) and is connected to a constant temperature connecting pipe (12). The four culture chambers (5) are connected to four ports outside the conducting assembly (11). A driving assembly (13) is fixedly installed on the bottom of the incubator (1), and the positions of the bottom of the culture rack (4) corresponding to the four culture chambers (5) are connected and installed with discharge valve pipes (14).

2. The modular cell culture device according to claim 1, characterized in that: The bottom end of the fixed column (2) is fixedly connected to a base (15), one side of the base (15) is fixed to the bottom of the drive assembly (13), a sealed door (16) is installed on the outside of the incubator (1) via a hinge, and a display module (17) and a control panel (18) are installed on the side of the outer wall of the incubator (1) corresponding to the sealed door (16).

3. The modular cell culture device according to claim 1, characterized in that: The culture module (6) includes a support plate (601), which is slidably connected to the inside of the culture chamber (5), and a plurality of anti-slip pads (602) are fixed on the support plate (601). A plurality of blocking bars (603) are equidistantly arranged around the support plate (601) corresponding to the anti-slip pads (602). The bottom of the culture vessel (7) overlaps the anti-slip pad (602), and the culture vessel (7) is located between the plurality of blocking bars (603). A plurality of through holes (604) are provided on the support plate (601), and two slide grooves (605) are respectively provided on both sides of the support plate (601), and the slide bar (8) is slidably connected in the slide groove (605).

4. The modular cell culture device according to claim 3, characterized in that: The locking assembly (10) includes a pull rod (101), one end of the pull rod (101) is fixedly connected to a handle (102), and the other end of the pull rod (101) is clamped in the clamping slot (9), the pull rod (101) is outer-connected with a sliding sleeve (103), the outer wall of the sliding sleeve (103) is fixed with a baffle (104), the baffle (104) is fixed to the bottom of the support plate (601), the pull rod (101) is outer-connected with a spring (105), and the two ends of the spring (105) are respectively fixed to one side of the bottom of the support plate (601) and the outside of the pull rod (101).

5. The modular cell culture device according to claim 1, characterized in that: The conduction assembly (11) includes a bearing seat (111), the bearing seat (111) is clamped on the top of the inner wall of the incubator (1), a rotating ring (112) is sleeved in the bearing seat (111), four connecting tubes (115) are fixed through the rotating ring (112), and the bottom of the rotating ring (112) is fixed to the top of the culture rack (4), one end of the connecting tube (115) passes through the top of the culture rack (4) and is connected to the culture chamber (5), and the other ends of the four connecting tubes (115) are connected to a rotating joint (113).

6. The modular cell culture device according to claim 5, characterized in that: The fixed end at the bottom of the rotary joint (113) is clamped on the top of the fixed column (2), and the movable end at the top of the rotary joint (113) is connected to the bottom end of the constant temperature connecting tube (12) and a sleeve (114) is fixed on the outer wall. The sleeve (114) passes through and rotates on the top of the incubator (1). A solenoid valve (116) is installed on the outer wall of the connecting tube (115) near the rotary joint (113). A wire (117) is connected to the solenoid valve (116). The wire (117) passes through the sleeve (114) and the incubator (1) and extends to the top of the incubator (1).

7. The modular cell culture device according to claim 1, characterized in that: The driving assembly (13) includes a limit bearing (131), the limit bearing (131) is clamped on the bottom of the incubator (1), a clamping ring (132) is sleeved inside the limit bearing (131), the top of the clamping ring (132) is fixed to the bottom of the incubator (4), a gear ring (133) is fixed to the bottom of the clamping ring (132), the gear ring (133) is externally meshed with a gear (134), a motor (135) is fixed to the bottom of the gear (134), the fixing column (2) is inserted through the middle of the clamping ring (132) and the gear ring (133), and the discharge valve pipe (14) is inserted through the clamping ring (132) and fixed in the clamping ring (132).

8. A method for using a modular assembled cell culture device, according to any one of claims 1 to 7, characterized in that: The method of use comprises the following steps: When culturing cells, the sealing door (16) is opened to expose the culture rack (4) and the culture chamber (5), and the multiple culture modules (6) in the culture chamber (5) are facing the opening of the incubator (1). The culture vessel (7) containing the cells is placed on the anti-slip mat (602) in the culture module (6). The anti-slip effect of the culture vessel (7) is improved by adding the anti-slip mat (602), and a plurality of blocking bars (603) are evenly arranged around the corresponding anti-slip mat (602) on the support plate (601). The outer side of the culture vessel (7) is blocked and limited by the plurality of blocking bars (603), thereby preventing the culture vessel (7) from deviating and colliding with each other; When adjusting the spacing between two adjacent support plates (601) to meet the culture space requirements of culture vessels (7) of different specifications, by pulling the handle (102) at the bottom of the support plate (601), the handle (102) drives the pull rod (101) to move forward, so that the end of the pull rod (101) will be separated from the slot (9) in the culture chamber (5). At this time, the support plate (601) is in a free moving state in the culture chamber (5). The support plate (601) is adjusted up and down to slide freely in the culture chamber (5). At the same time, the slide grooves (605) on both sides of the support plate (601) slide outside the slide bar (8), which plays a role in supporting and limiting the support plate (601), preventing the support plate (601) from shifting position during movement and preventing the support plate (601) from being separated from the culture chamber (5). ), improve the stability of the adjustment of the support plate (601), after adjusting the support plate (601) to the appropriate position, loosen the handle (102), support the pull rod (101) by the elastic force of the spring (105), make the pull rod (101) slide outside the sliding sleeve (103), limit the pull rod (101) by the sliding sleeve (103), improve the stability of the horizontal movement of the pull rod (101), until the end of the pull rod (101) is stuck in the card slot (9), and the purpose of locking the support plate (601) is achieved. At this time, the distance between the two adjacent support plates (601) changes. If the position of other support plates (601) is continued to be adjusted, the same operation is performed, thereby meeting the need to adjust the culture space of culture vessels (7) of different specifications; When adjusting the positions of the culture modules (6) in the other three culture chambers (5), the control panel (18) is operated outside the incubator (1) to control the motor (135) to work, and the output shaft of the motor (135) drives the gear (134) to rotate, and the gear (134) drives the retaining ring (132) to rotate inside the limiting bearing (131) through the gear ring (133), and the retaining ring (132) drives the culture rack (4) above to rotate, and the culture rack (4) drives the two supporting bearings (3) to rotate outside the fixed column (2), and the retaining ring (132) is supported by the limiting bearing (131), and the supporting bearing (3) supports the culture rack (4), thereby improving the stability of the culture rack (4) driven by the retaining ring (132). , the culture module (6) equipped with the culture vessel (7) is moved to one side of the incubator (1) and is blocked by the incubator (1) so that it will not be exposed to the outside. After the culture rack (4) is rotated ninety degrees, another culture chamber (5) is moved to the opening on the front of the incubator (1). Similarly, the position of the culture module (6) in the current culture chamber (5) is adjusted. In this way, the four culture chambers (5) are all independently designed. By placing different culture vessels (7) in different culture chambers (5), and integrating multiple culture chambers (5) in a single incubator (1), after completing the placement of the culture vessels (7), the sealing door (16) is closed to seal the opening on the front of the incubator (1), so that different types of cells can be cultured together; During the culture process, the constant temperature connection tube (12) is connected to the external constant temperature device, and the medium is introduced into the rotary joint (113) through the constant temperature tube. The movable end of the top of the rotary joint (113) is connected to the constant temperature connection tube (12). When the culture rack (4) drives the rotating ring (112) to rotate inside the bearing seat (111), the rotating ring (112) drives the movable end of the top of the rotary joint (113) to rotate through the four connecting tubes (115), without affecting the normal conduction between the constant temperature connection tube (12) and the rotary joint (113). During this process, the culture rack (4), the rotating ring (112), the connecting tube (115) and the sleeve (114) on the rotary joint (113) rotate synchronously, so that the connecting tube (115) will input the medium into the culture chamber (5) connected thereto, and by controlling the solenoid valve (116) outside the connecting tube (115), the environment in different culture chambers (5) can be independently adjusted. The wire (117) on the top of the solenoid valve (116) passes through the sleeve (114) and extends to the outside of the incubator (1) to energize the solenoid valve (116). After the medium enters the culture chamber (5), it will be introduced into the lower layer through the through hole (604) on the support plate (601), thereby improving the constant temperature effect inside the culture chamber (5). After opening the discharge valve tube (14) at the bottom of the culture rack (4), the medium inside the culture chamber (5) will be discharged, thereby improving the fluidity of the medium inside the culture chamber (5).

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