Multi-parameter visible component detection chip and device

Abstract

The multi-parameter visible component detection chip and the multi-parameter visible component detection device are used for detecting visible components of biological blood, urine or excrement, and a sample adding port is respectively communicated with a detection liquid containing cavity A and a detection liquid containing cavity B; the liquid containing height of the detection liquid containing cavity A is higher than that of the detection liquid containing cavity B; at least one of the upper part or the lower part of the detection liquid accommodating cavity A is made of a transparent material; at least one of the upper part or the lower part of the detection liquid accommodating cavity B is made of a transparent material; the detection liquid accommodating cavity A is used for measuring a detection parameter A; and the detection liquid accommodating cavity B is used for measuring a detection parameter B. The detection liquid accommodating cavities with different heights are used for measuring different detection parameters; the detection efficiency is improved, and especially the efficiency of multi-parameter visible component analysis can be improved.

Description

Technical Field

[0001] This application is a divisional application of application number CN2023112721255, and the application date of the parent application is September 28, 2023.

[0002] The present application relates to a chip for forming element analysis used for carrying a sample mixture to be imaged microscopically, and in particular to a chip and device for forming element analysis with multiple parameters. Background Art

[0003] In the prior art, such as Figure 16 , is a blood cell counting chamber. A blood cell counting chamber is a commonly used cell counting tool. It is named after it is often used to count red blood cells, white blood cells, etc. in medicine. It is also commonly used to calculate the number of microorganisms such as bacteria, fungi, and yeast. It is a common biological tool.

[0004] Hemocytometers are made of thick glass. Each chamber is divided into two identical counting chambers by an H-shaped groove. A cover glass is placed on top, forming a 0.10 mm high counting chamber. The counting chamber is marked with a grid of 3.0 mm long and wide, divided into nine large squares. Each large square measures 1.0 mm x 1.0 mm = 1.0 mm²; the volume is 1.0 mm² x 0.1 mm = 0.1 mm³.

[0005] Figure 17 、 Figure 18 、 Figure 19 , is a series of algae counters and biological tools. Depending on the concentration of the test object, different counting chambers with different depths are required. During the test process, the counting chamber needs to be replaced multiple times according to the test results or the test object. Summary of the Invention

[0006] The technical problem to be solved by the present application is that when detecting different objects or tangible components of different concentrations, different sample volumes or sample sedimentation heights are required, which leads to the need for a large number of detection holding devices. The present application proposes to integrate sample holding cavities of different capacities and heights into a detection holding device, so that one device can cope with the detection of different detection objects or detection objects of different concentrations. The present invention can be applied to the detection process of all biological detection objects.

[0007] The present application provides a multi-parameter shaped component detection and analysis chip and device, comprising two or more detection liquid holding cavities. Different detection liquid holding cavities have different heights, corresponding to different detection liquid volumes within them, and correspondingly different probabilities of different components of shaped components appearing within their unit imaging area. By setting different detection liquid holding cavities at different heights, the detection liquid volume used for imaging is adjusted, and the probability of different components appearing in the image is adjusted, thereby reducing the workload of subsequent image acquisition and improving the efficiency of image acquisition and data analysis.

[0008] The technical solution for solving the above-mentioned technical problems in the present application is a multi-parameter formed element detection chip, which is used for the detection of formed elements in biological blood, urine, feces or body fluids, and includes a detection liquid holding cavity A, a detection liquid holding cavity B, and a sampling port; the sampling port is used to add the sample to be tested; the sampling port is connected to the detection liquid holding cavity A; the sampling port is connected to the detection liquid holding cavity B; the liquid holding height of the detection liquid holding cavity A is higher than the liquid holding height of the detection liquid holding cavity B; at least one of the upper or lower parts of the detection liquid holding cavity A is made of transparent material; at least one of the upper or lower parts of the detection liquid holding cavity B is made of transparent material; the detection liquid holding cavity A is used to measure the detection parameter A; the detection liquid holding cavity B is used to measure the detection parameter B.

[0009] The detection liquid containing cavity A and the detection liquid containing cavity B are connected in series, and the liquid to be detected is poured into the detection liquid containing cavity A and the detection liquid containing cavity B in sequence.

[0010] The detection liquid holding cavity A and the detection liquid holding cavity B are connected in series to form a first detection channel; and further include a detection liquid holding cavity A2 and a detection liquid holding cavity B2, for sequentially pouring the liquid to be tested into the detection liquid holding cavity A2 and the detection liquid holding cavity B2; the liquid holding height of the detection liquid holding cavity A2 is higher than the liquid holding height of the detection liquid holding cavity B2, and the detection liquid holding cavity A2 and the detection liquid holding cavity B2 are connected in series to form a second detection channel.

[0011] The detection liquid containing cavity A and the detection liquid containing cavity B are connected in parallel, so that the liquid to be detected is poured into the detection liquid containing cavity A and the detection liquid containing cavity B at the same time.

[0012] The multi-parameter shaped component detection chip further includes a detection liquid holding cavity C and a detection liquid holding cavity D; the detection liquid holding cavity A, the detection liquid holding cavity B, the detection liquid holding cavity C and the detection liquid holding cavity D are distributed in equal heights.

[0013] The multi-parameter shaped component detection chip includes any one of the following technical features: Feature T10, the detection liquid holding cavity A and the detection liquid holding cavity B have bottoms at the same height; Feature T20, the detection liquid holding cavity A and the detection liquid holding cavity B have tops at the same height.

[0014] The multi-parameter formed element detection chip includes any one of the following technical features: Feature TA10, the detection liquid containing cavity A is used to detect white blood cells in the blood of a living being; Feature TA20, the detection liquid containing cavity B is used to detect red blood cells in the blood of a living being; Feature TA30, the detection liquid containing cavity B is used to detect platelets in the blood of a living being; Feature TB10, the detection liquid containing cavity A is used to detect insect eggs in the fecal suspension of a living being; Feature TB20, the detection liquid containing cavity B is used to detect bacteria in the fecal suspension of a living being; Feature TB30, the detection liquid containing cavity B is used to detect cells in the fecal suspension of a living being; Feature TC10, the detection liquid containing cavity A is used to detect tubular objects in the urine of a living being; Feature TC20, the detection liquid containing cavity A is used to detect crystals in the urine of a living being; Feature TC30, the detection liquid containing cavity B is used to detect cells in the urine of a living being; Feature TC40, the detection liquid containing cavity B is used to detect bacteria in the urine of a living being.

[0015] The detection liquid holding cavity A or the detection liquid holding cavity B is formed by the bottom plate and the upper plate, and the detection liquid holding cavity A or the detection liquid holding cavity B is formed by the concave portion of the upper plate; or the detection liquid holding cavity A or the detection liquid holding cavity B is formed by the concave portion of the lower plate; the bottom plate and the upper plate are welded, bonded or ultrasonically welded together; and further include an exhaust port; the exhaust port is connected to the detection liquid holding cavity A; the exhaust port is connected to the detection liquid holding cavity B; and the exhaust port is connected to the external atmosphere.

[0016] The technical solution for solving the above technical problems in the present application can also be a multi-parameter shaped component detection device, including a camera component, a shaped component detection component, and a carrying component; the carrying component is used to carry the multi-parameter shaped component detection chip; the carrying component includes an XY moving component and a chip clamping component; the chip clamping component is used to fix the multi-parameter shaped component detection chip; the camera component includes a slide module and a camera module; the shaped component detection component includes a focus control module, an image acquisition module, and a multi-parameter chip motion control component; the multi-parameter shaped component detection chip includes a test sample holding cavity, and the test sample holding cavity includes a test liquid holding cavity A and a test liquid holding cavity B; the test sample holding cavity is used to hold a sample to be tested, and the test liquid holding cavity A and the test liquid holding cavity B are connected to each other, and the cavity height of the test liquid holding cavity A is higher than the cavity height of the test liquid holding cavity B; the slide module adjusts the camera The distance between the imaging module and the multi-parameter formed component detection chip; the camera module is used to capture the image of the sample to be detected inside the detection sample holding cavity; the focus control module controls the slide module to adjust the distance between the camera module and the multi-parameter formed component detection chip; the image acquisition module acquires the image captured by the camera module; the multi-parameter chip motion control component controls the XY moving component to drive the multi-parameter formed component detection chip to move, so that the detection liquid holding cavity A is aligned with the camera module, and the image acquisition module acquires the image of the detection liquid holding cavity A, and the image of the detection liquid holding cavity A is used to measure the detection parameter A; the multi-parameter chip motion control component controls the XY moving component to drive the multi-parameter formed component detection chip to move, so that the detection liquid holding cavity B is aligned with the camera module, and the image acquisition module acquires the image of the detection liquid holding cavity B, and the image of the detection liquid holding cavity B is used to measure the detection parameter B.

[0017] The multi-parameter formed element detection device includes any one of the following technical features: feature TAA10, detection parameter A is for detecting white blood cells in biological blood; feature TAA20, detection parameter B is for detecting red blood cells in biological blood; feature TAA30, detection parameter B is for detecting platelets in biological blood; feature TBB10, detection parameter A is for detecting insect eggs in biological fecal suspension; feature TBB20, detection parameter B is for detecting bacteria in biological fecal suspension; feature TBB30, detection parameter B is for detecting cells in biological fecal suspension; feature TCC10, detection parameter A is for detecting tubular objects in biological urine; feature TCC20, detection parameter B is for detecting crystals in biological urine; feature TCC30, detection parameter B is for detecting cells in biological urine; feature TCC40, detection parameter A is for detecting bacteria in biological urine.

[0018] The multi-parameter shaped component detection device includes any one of the following technical features: Feature TDD10, if the target objects to be detected are found to be superimposed in multiple layers in the detection liquid containing cavity A, the detection of the target objects to be detected is switched to the detection liquid containing cavity B; Feature TDD20, if the target objects to be detected are not found in the detection liquid containing cavity B, the detection of the target objects to be detected is switched to the detection liquid containing cavity A; Feature TDD30, if the target objects to be detected are not found in the detection liquid containing cavity A or the number of the target objects to be detected is small, the detection of the target objects to be detected is switched to the detection liquid containing cavity B.

[0019] The multi-parameter formed element detection device includes any one of the following technical features: Feature TEE10, the target to be detected is red blood cells in the blood of a living being; Feature TEE11, the target to be detected is white blood cells in the blood of a living being; Feature TEE12, the target to be detected is platelets in the blood of a living being; Feature TEE21, the target to be detected is insect eggs in the fecal suspension of a living being; Feature TEE22, the target to be detected is bacteria in the fecal suspension of a living being; Feature TEE23, the target to be detected is cells in the fecal suspension of a living being; Feature TEE50, the target to be detected is cells in the urine of a living being; Feature TEE51, the target to be detected is bacteria in the urine of a living being; Feature TEE52, the target to be detected is tubular objects in the urine of a living being; Feature TEE53, the target to be detected is crystals in the urine of a living being.

[0020] First, an image of the detection liquid holding cavity B is acquired, and detection parameter B is measured. After parameter B is measured, an image of the detection liquid holding cavity A is acquired, and detection parameter A is measured.

[0021] The beneficial effects of the technical solution of this application include: having two or more detection liquid holding cavities; different detection liquid holding cavities having different heights, corresponding to different detection liquid volumes within them, and correspondingly different probabilities of different components appearing within a unit imaging area. By setting different detection liquid holding cavities at different heights, the detection liquid volume used for imaging is adjusted, thereby adjusting the probability of different components appearing in the image, reducing the workload of subsequent image acquisition and improving the efficiency of image acquisition and data analysis.

[0022] The beneficial effects of the technical solution in the present application include: detection liquid holding cavities of different heights are used for measuring different detection parameters; one chip can detect two parameters at the same time. For example, the number of red blood cells and white blood cells in the blood is an order of magnitude different. In one chip, two different heights can correspond to red blood cells and white blood cells. One chip can adapt to the detection characteristics of two different parameters, which greatly reduces the difficulty and time of detection.

[0023] The beneficial effects of the technical solution in the present application include: achieving enrichment of visible elements of different concentrations in the sample by detecting the height difference between the liquid containing cavities, thereby reducing the burden on the number of microscopic magnification images obtained due to the concentration difference between different visible elements.

[0024] The beneficial effects of the technical solution of this application include: the detection liquid holding chamber A and the detection liquid holding chamber B are connected in series, and the liquid to be tested can be poured into the detection liquid holding chamber A and the detection liquid holding chamber B in sequence. Only one injection operation is required to complete the injection of the liquid to be tested into different chambers.

[0025] The beneficial effects of the technical solution in the present application include: the detection liquid holding cavity A and the detection liquid holding cavity B are connected in series to form a first detection channel; the detection liquid holding cavity A2 and the detection liquid holding cavity B2 are connected in series to form a second detection channel; the two detection channels increase the number of samples tested at one time, thereby improving the utilization efficiency of the detection equipment.

[0026] The beneficial effects of the technical solution in the present application include: the detection liquid holding chamber A and the detection liquid holding chamber B are connected in parallel, and the liquid to be tested is simultaneously poured into the detection liquid holding chamber A and the detection liquid holding chamber B; the sample is allowed to quickly enter the two parallel channels, and the low-height holding chamber can also prevent large pieces of sample from entering. For example, during feces testing, large objects can be blocked outside channel B, allowing cells or bacteria to enter the detection channel B.

[0027] The beneficial effects of the technical solution in the present application include: the detection liquid holding cavity A and the detection liquid holding cavity B have bottoms of the same height, or tops of the same height; it is convenient for the manufacture of multi-parameter shaped component detection chips, and also convenient for focus control.

[0028] The beneficial effects of the technical solution in this application include: being able to analyze different formed elements in the blood; the detection liquid containing chamber A is used for white blood cell detection and analysis, and the detection liquid containing chamber B is used for red blood cell or platelet analysis, and this combination is highly efficient.

[0029] The beneficial effects of the technical solution in this application include: being able to analyze different formed components in blood and feces suspensions; the detection liquid containing cavity A is used for worm egg detection and analysis, and the detection liquid containing cavity B is used for bacteria or cell analysis, and this combination is highly efficient.

[0030] The beneficial effects of the technical solution in the present application include: being able to analyze different formed elements in urine; the detection liquid containing cavity A is used for the detection and analysis of tubular objects or crystals, and the detection liquid containing cavity B is used for bacteria or cell analysis, and this combination is highly efficient.

[0031] The beneficial effects of the technical solution in the present application include: the detection liquid containing cavity A or the detection liquid containing cavity B is formed by enclosing the bottom plate and the upper plate, and the detection liquid containing cavity A or the detection liquid containing cavity B is formed by the concave portion of the upper plate.

[0032] The beneficial effects of the technical solution in this application include: the bottom plate and the upper plate are welded, bonded or ultrasonically welded into one, which facilitates manufacturing and reduces costs.

[0033] The beneficial effects of the technical solution in the present application include: the exhaust port is connected to the detection liquid holding cavity A; the exhaust port is connected to the detection liquid holding cavity B; the exhaust port is connected to the external atmosphere, which facilitates the rapid and bubble-free uniform distribution of the detection liquid in the detection liquid holding cavity.

[0034] The beneficial effects of the technical solution in the present application include: a multi-parameter formed component detection device improves image acquisition efficiency by acquiring images of the detection liquid holding cavity at different heights and obtaining microscopic magnified images calculated by corresponding detection parameter analysis.

[0035] The beneficial effects of the technical solution in the present application include: detection liquid holding cavities of different heights correspond to different detection liquid holding volumes. If the detection liquid volume multiples corresponding to detection liquid holding cavities A and B are N, the efficiency of acquiring images is increased by N times, and the number of images acquired corresponding to detection liquid holding cavity A can be 1 / N of the original.

[0036] The beneficial effects of the technical solution in the present application include: even if only one parameter is tested each time, cavities of different heights can correspond to different parameters, which greatly reduces the number of chips. For example, there are cavities of four heights, corresponding to the detection of four parameters. Even if only one parameter is tested each time, overall, only one chip needs to be produced. If one height corresponds to the detection of several parameters, the number of parameter detection types corresponding to the four heights is far greater than four.

[0037] The beneficial effects of the technical solution in the present application include: flexible switching between the detection liquid holding cavity A and the detection liquid holding cavity B; flexible switching between different detection liquid holding cavities according to the actual distribution of the target objects to be detected, thereby improving the adaptability of detection.

[0038] The beneficial effects of the technical solution of this application include: first measuring the test parameter B, then measuring the test parameter A. The height of the test liquid holding cavity B is relatively low, so the formed components reach a stable state more quickly. Testing parameter B is performed first. During the process of photographing and testing the holding cavity B, the formed components in the test liquid holding cavity A gradually stabilize, and the test of the holding cavity B is completed before the holding cavity A is tested, which can significantly reduce the test time. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 11 is a schematic top view of the structure of a multi-parameter formed component detection chip according to embodiment 1; Figure 2 yes Figure 1 The cross-sectional structure diagram of the AA section; Figure 3 1 is a schematic top view of the structure of a multi-parameter formed component detection chip according to embodiment 2; Figure 4 yes Figure 3 The cross-sectional structure diagram of the middle BB section; Figure 5 is a three-dimensional diagram of embodiment 3 of the multi-parameter formed component detection chip; Figure 6 1 is a perspective schematic diagram of Example 3 of a multi-parameter formed component detection chip; Figure 7 is a perspective view of the upper plate 910 in the multi-parameter formed component detection chip; Figure 8 yes Figure 7 The cross-sectional view after removing the right part of the CC section; Figure 9 yes Figure 6 Schematic cross-sectional view of the CC section; Figure 10 yes Figure 9 A local enlarged schematic diagram in FIG. Figure 11 1 is a top view schematic diagram of a multi-parameter formed component detection chip embodiment 4; Figure 12 1 is a schematic diagram of a decomposed state of a multi-parameter formed component detection chip according to Example 4; Figure 13 yes Figure 11 Partial schematic diagram after removing the upper part of the DD section; Figure 14 yes Figure 11 Schematic cross-sectional view of the middle DD section; Figure 15 It is a schematic block diagram of a multi-parameter formed element detection device; Figure 16 It is a schematic diagram of the grid of a blood cell counting chamber; Figure 17 There are three seaweed counters of different sizes; Figure 18 This is a table of dimensions and specifications for different seaweed counters; different enclosure depths correspond to different capacities; Figure 19 This is a table showing the applicable ranges of seaweed counters of different specifications. DETAILED DESCRIPTION

[0040] The contents of this application are further described in detail below in conjunction with the accompanying drawings. It should be noted that the following is a description of the preferred embodiments of the present invention and does not constitute any limitation to the present invention. The description of the preferred embodiments of the present invention is only an illustration of the general principles of the present invention. The numbers such as "first", "second" and "A" and "B" involved in the present invention are only for the convenience of explanation and do not represent the order relationship in time or space. The combination of letters and numbers involved in the present invention is only for the convenience of explanation, and the specific meaning is determined by the specific words referred to. In this application document, the "features" and numbers in the claims, such as "TAA10", are only for the convenience of statement and do not have any substantial limitation or interpretation on the scope of the claims.

[0041] Formed ingredient detection chips used for microscopic imaging typically have only a single detection fluid chamber, making it impossible to analyze and detect two different samples simultaneously. To calculate and analyze relevant parameters for the same sample, a chip with only a single detection fluid chamber must acquire a corresponding number of magnified microscopic images based on the probability of the current parameter appearing in the microscopic image.

[0042] For example, in a formed element detection chip used for blood analysis, both white blood cell and red blood cell counts must be analyzed simultaneously. However, in a typical human blood sample, the number of red blood cells is hundreds or even thousands of times greater than the number of white blood cells. To analyze red blood cells, one or two images are typically required, and the number of red blood cells in each image can be statistically analyzed to obtain red blood cell concentration data. However, due to the low white blood cell count, quantitative measurement or morphological analysis of the obtained white blood cell count is impossible with a small number of images, given the same sample size (corresponding to the number of images).

[0043] To obtain a sufficient number of WBC images, many images must be taken. In actual products, capturing and analyzing these images consumes considerable time and computing resources. However, increasing the sample size, the amount of WBC sediment, and the degree of WBC enrichment through physical methods can significantly reduce the number of WBC images required. For example, if the height of the WBC detection chamber is four times that of the RBC detection chamber, the WBC enrichment capacity is four times that of the RBC.

[0044] like Figure 1 and Figure 2As shown, an embodiment of a multi-parameter tangible element detection chip 101 for detecting tangible elements in biological blood, urine, feces, or body fluids includes a detection liquid holding chamber A200, a detection liquid holding chamber B300, and a sample injection port 150. The sample injection port 150 is used to add a sample to be tested; the sample injection port 150 is connected to the detection liquid holding chamber A200; the sample injection port 150 is connected to the detection liquid holding chamber B300; the liquid holding height of the detection liquid holding chamber A200 is higher than the liquid holding height of the detection liquid holding chamber B300; at least one of the upper or lower portions of the detection liquid holding chamber A200 is made of a transparent material; at least one of the upper or lower portions of the detection liquid holding chamber B300 is made of a transparent material; the detection liquid holding chamber A200 is used to measure detection parameter A; the detection liquid holding chamber B300 is used to measure detection parameter B. Body fluids include saliva, semen, various cavity fluids, and tissue fluids.

[0045] like Figure 1 and Figure 2 As shown, in an embodiment of a multi-parameter formed component detection chip, the upper portion of the detection liquid containing cavity A200 and the upper portion of the detection liquid containing cavity B300 are made of transparent materials.

[0046] In embodiments of the multi-parameter shaped component detection chip not shown in other figures, the lower portions of detection liquid holding chamber A200 and the lower portions of detection liquid holding chamber B300 are made of transparent material. To facilitate microscopic imaging, the lower portions of detection liquid holding chambers A200 and B300 are typically aligned and positioned on the same plane, facilitating imaging and observation.

[0047] During the microscopic imaging process, illumination is a very important test environment requirement. The use of transparent materials can facilitate the entry of light into the detection liquid holding cavity. Depending on different lighting arrangements, the surrounding materials of the detection liquid holding cavity can be made of different materials.

[0048] Likewise, the upper portion of the detection liquid containing cavity A200 and the upper portion of the detection liquid containing cavity B300 may also be flush with each other and arranged on the same plane to facilitate imaging and observation.

[0049] By setting different test liquid holding chambers at different heights, different volumes of the same sample can be injected with just one injection of the test liquid (or test sample). This height difference in the test liquid holding chambers allows for the enrichment of low-concentration visible components in the sample, reducing the burden on the number of microscopic images acquired due to concentration differences between visible components. In the case of dual camera assemblies, simultaneous imaging of different transparent areas on the same side can also be performed, further improving the efficiency of microscopic image acquisition.

[0050] In some embodiments of multi-parameter tangible component detection chips not shown in the accompanying drawings, the upper portion of detection liquid holding chamber A200 and the lower portion of detection liquid holding chamber B300 are made of transparent materials; alternatively, the lower portion of detection liquid holding chamber A200 and the upper portion of detection liquid holding chamber B300 are made of transparent materials. The transparent positions of different cavities on the multi-parameter tangible component detection chip can be staggered. In the case of dual-camera assemblies, imaging of different transparent portions on different sides can be performed simultaneously. For example, if the upper portion of detection liquid holding chamber A200 is made of transparent material, imaging of detection liquid holding chamber A200 is performed from the upper side; if the lower portion of detection liquid holding chamber B300 is made of transparent material, imaging of detection liquid holding chamber B300 is performed from the lower side, further improving imaging efficiency. When the parameters being detected have different density characteristics, flexible selection can be made based on the different density characteristics. If some tangible components float upward, the detection liquid holding chamber with a transparent upper portion can be selected. If some components sink, a transparent detection liquid holding cavity is selected at the bottom to effectively reduce the focusing time, further improve the efficiency of obtaining microscopic images of formed components, and improve the efficiency of identifying parameters of formed components.

[0051] like Figure 1 and Figure 2 In an embodiment of a multi-parameter formed ingredient detection chip, detection liquid chamber A200 and detection liquid chamber B300 are connected in series, allowing the detection liquid to be sequentially poured into detection liquid chamber A200 and detection liquid chamber B300. A single injection operation is sufficient to complete the injection of the detection liquid into different chambers.

[0052] like Figures 1 to 2 As shown, in an embodiment of the multi-parameter formed component detection chip, the multi-parameter formed component detection chip further includes an exhaust port 160; the exhaust port 160 is connected to the detection liquid holding cavity A200; the exhaust port is connected to the detection liquid holding cavity B30; the exhaust port 160 is connected to the external atmosphere.

[0053] In some embodiments of the multi-parameter formed component detection chip not shown in the accompanying drawings, the detection liquid holding cavity A and the detection liquid holding cavity B are connected in parallel, so that the liquid to be detected is simultaneously poured into the detection liquid holding cavity A and the detection liquid holding cavity B.

[0054] like Figure 3 and Figure 4As shown, in an embodiment of a multi-parameter formed component detection chip 500, it includes a sample addition port 550 and an exhaust port 560; the detection liquid containing cavity A501, the detection liquid containing cavity B502, the detection liquid containing cavity C503, the detection liquid containing cavity D504, and the detection liquid containing cavity F505 are connected in parallel; the detection liquid containing cavity A501, the detection liquid containing cavity B502, the detection liquid containing cavity C503, the detection liquid containing cavity D504, and the detection liquid containing cavity F505 are distributed in an equidistant manner in terms of height.

[0055] In some embodiments of multi-parameter formed component detection chips, the number of detection liquid holding cavities can be 2, 3, 4, or even more. The specific number of cavities can be set according to the different types of formed components; the number of detection liquid holding cavities can be set according to the number of formed components to be tested.

[0056] like Figure 2 In an embodiment of a multi-parameter shaped ingredient detection chip, detection liquid holding chamber A200 and detection liquid holding chamber B300 have bottoms at the same height. That is, the bottoms of detection liquid holding chamber A200 and detection liquid holding chamber B300 are flush and coplanar. Having the same bottom height facilitates the passage of shaped ingredients.

[0057] In some embodiments of the multi-parameter shaped component detection chip not shown in the accompanying drawings, the detection liquid holding chamber A and the detection liquid holding chamber B have tops at the same height. The specific arrangement can be determined based on the position of the camera assembly and the density characteristics of the specific shaped component to be detected.

[0058] In some embodiments of the multi-parameter shaped component detection chip not shown in the accompanying drawings, the detection liquid holding cavity A and the detection liquid holding cavity B may also have bottoms of different heights; the detection liquid holding cavity A and the detection liquid holding cavity B may also have tops of different heights.

[0059] like Figure 1 and Figure 2 As shown, an embodiment of a multi-parameter formed element detection chip is used to detect formed elements in a living being's blood. Detection fluid chamber A is used to detect white blood cells in the living being's blood, while detection fluid chamber B is used to detect red blood cells in the living being's blood. This configuration is sufficient when only white blood cells and red blood cells are required for detection. If platelet detection is also required, detection fluid chamber B can also be used to detect platelets in the living being's blood. Of course, if red blood cell detection is not required, the combination of detection fluid chambers A and B allows detection of only white blood cells and platelets.

[0060] In some embodiments of the multi-parameter formed element detection chip not shown in the accompanying drawings, the detection liquid holding chamber A is used to detect white blood cells in the blood of a living being; the detection liquid holding chamber B is used to detect red blood cells in the blood of a living being; and the detection liquid holding chamber B is used to detect platelets in the blood of a living being.

[0061] In some embodiments of the multi-parameter visible element detection chip (not shown in the accompanying figures), detection fluid chamber A is used to detect insect eggs in a fecal suspension; detection fluid chamber B is used to detect bacteria or cells in the fecal suspension. The relationship between the combination of insect eggs, bacteria, or cells is similar to the relationship between the different visible elements in blood.

[0062] In some embodiments of the multi-parameter formed element detection chip, not shown in the accompanying figures, detection fluid chamber A is used to detect casts or bacteria in biological urine, while detection fluid chamber B is used to detect cells or crystals in biological urine. The relationship between casts, bacteria, and cells or crystals is similar to the relationship between different formed elements in blood.

[0063] In some embodiments of the multi-parameter formed element detection chip, not shown in the accompanying figures, detection fluid chamber A is used to detect casts or crystals in biological urine, while detection fluid chamber B is used to detect cells or bacteria in biological urine. The relationship between casts or crystals and cells or bacteria is similar to the relationship between different formed elements in blood.

[0064] In some embodiments of the multi-parameter formed element detection chip, not shown in the accompanying drawings, the detection fluid holding chamber A is used to detect casts or crystals in biological urine; detection fluid holding chamber A is also used to detect cells or bacteria in biological urine. In raw urine, the distribution of various formed elements is relatively dispersed, and the solution in this application can be used for analysis based on raw urine samples. Detection fluid holding chamber A has a relative enrichment effect on formed elements. Of course, the technical solution in this application is not only applicable to the analysis of raw urine samples, but also to urine samples based on urine concentration or urine sediment treatment.

[0065] In some embodiments of the multi-parameter shaped component detection chip not shown in the accompanying drawings, feature TCC10, detection parameter A is detecting tubular objects in biological urine; like Figures 5 to 10As shown, in an embodiment of a multi-parameter formed ingredient detection chip, a multi-parameter formed ingredient detection chip 901 includes a detection liquid holding chamber A201 and a detection liquid holding chamber B301 connected in series to form a first detection channel. The first detection channel is correspondingly provided with a first detection channel sample port 951 and a first detection channel exhaust port 961. The first detection channel sample port 951 is connected to the detection liquid holding chamber A201 and the detection liquid holding chamber B301; the first detection channel exhaust port 961 is also connected to the detection liquid holding chamber A201 and the detection liquid holding chamber B301.

[0066] like Figures 5 to 10 As shown, in an embodiment of a multi-parameter shaped component detection chip, the multi-parameter shaped component detection chip 901 further includes a detection liquid holding cavity A2 and a detection liquid holding cavity B2, which are used to sequentially pour the liquid to be detected into the detection liquid holding cavity A2 and the detection liquid holding cavity B2; the detection liquid holding cavity A2 is labeled 202, and the detection liquid holding cavity B2 is labeled 302. The liquid holding height of the detection liquid holding cavity A2 is higher than the liquid holding height of the detection liquid holding cavity B2, and the detection liquid holding cavity A2 and the detection liquid holding cavity B2 are connected in series to form a second detection channel. The second detection channel is correspondingly provided with a second detection channel sample port 952 and a second detection channel exhaust port 962. The second detection channel sample port 952 is in communication with the detection liquid holding cavity A2 and the detection liquid holding cavity B2; the second detection channel exhaust port 962 is also in communication with the detection liquid holding cavity A2 and the detection liquid holding cavity B2. In Figures 5 to 10 In the embodiment, the first detection channel and the second detection channel are independent of each other and are not interconnected. This facilitates the simultaneous analysis of multiple different types of samples, and also facilitates the simultaneous analysis of the same type of samples with different dilution or concentration ratios. Figure 9 and Figure 10 As shown, the height of the detection liquid holding cavity A201 is greater than the height of the detection liquid holding cavity B301. The specific height is set according to the type of target detection parameter or sample type.

[0067] like Figures 5 to 10 As shown, in the embodiment of the multi-parameter formed component detection chip, the detection liquid holding cavity A or the detection liquid holding cavity B is formed by the bottom plate 920 and the upper plate 910, and the detection liquid holding cavity A or the detection liquid holding cavity B is formed by the upper plate 910 being concave. In other embodiments not shown in the drawings, the detection liquid holding cavity A or the detection liquid holding cavity B is formed by the bottom plate 920 being concave. Figures 5 to 10As shown, in an embodiment of a multi-parameter formed component detection chip, the lower portion of the upper plate 910 is provided with recesses corresponding to the first and second detection channels. Recess A 9201 in the first detection channel corresponds to the position of detection liquid holding chamber A201, and recess B 9202 in the first detection channel corresponds to the position of detection liquid holding chamber B301. The bottom plate 920 and the upper plate 910 are welded, bonded, or ultrasonically welded together.

[0068] like Figure 9 and Figure 10 As shown, in the embodiment of the multi-parameter formed component detection chip, the detection liquid holding cavity A201 and the detection liquid holding cavity B301 with different cavity heights are connected and are arranged between the first detection channel exhaust port 961 and the first detection channel sample port 951.

[0069] like Figures 11 to 14 As shown, in an embodiment of a multi-parameter formed component detection chip 800, the detection liquid holding chamber is formed by a base plate 820 and an upper plate 810. The five parallel detection liquid holding chambers are a first detection liquid holding chamber A801, a second detection liquid holding chamber A802, a third detection liquid holding chamber A803, a fourth detection liquid holding chamber A804, and a fifth detection liquid holding chamber A805. These five parallel detection liquid holding chambers share the same sample loading port 850 and exhaust port 860. The upper plate 810 is provided with a first recess 8501, a second recess 8502, a third recess 8503, a fourth recess 8504, and a fifth recess 8505, each of which is positioned corresponding to a corresponding detection liquid holding chamber. The number of detection liquid holding chambers can be two, three, four, five, or even more. At least two detection liquid holding cavities can adjust the detection liquid volume multiple once when the imaging area of ​​each detection liquid holding cavity is the same, thereby improving the efficiency of obtaining microscopic images. In some embodiments, the heights of the first to fifth detection liquid holding cavities A are 0.1mm, 0.2mm, 0.3mm, 0.4mm, and 0.5mm, respectively, and are designed in equal steps. Figures 11 to 14 In the illustrated embodiment, the heights of the first to fifth testing liquid containing cavities A are 0.1 mm, 0.2 mm, 0.5 mm, 0.3 mm, and 0.4 mm, respectively.

[0070] like Figure 15 and Figure 1As shown, in an embodiment of a multi-parameter shaped component detection device, it includes a camera component, a shaped component detection component, and a carrying component; the carrying component is used to carry the multi-parameter shaped component detection chip; the carrying component includes an XY moving component and a chip clamping component; the chip clamping component is used to fix the multi-parameter shaped component detection chip; the camera component includes a slide module and a camera module; the shaped component detection component includes a focus control module, an image acquisition module, and a multi-parameter chip motion control component; the multi-parameter shaped component detection chip includes a test sample holding cavity, and the test sample holding cavity includes a test liquid holding cavity A and a test liquid holding cavity B; the test sample holding cavity is used to hold a sample to be tested, and the test liquid holding cavity A and the test liquid holding cavity B are connected to each other, and the cavity height of the test liquid holding cavity A is higher than the cavity height of the test liquid holding cavity B; the slide module adjusts the camera module and the multi-parameter The multi-parameter chip motion control component controls the XY moving component to drive the multi-parameter formed component detection chip to move, so that the detection liquid holding cavity A is aligned with the camera module, and the image acquisition module acquires the image of the detection liquid holding cavity A, and the image of the detection liquid holding cavity A is used to measure the detection parameter A; the multi-parameter chip motion control component controls the XY moving component to drive the multi-parameter formed component detection chip to move, so that the detection liquid holding cavity B is aligned with the camera module, and the image acquisition module acquires the image of the detection liquid holding cavity B, and the image of the detection liquid holding cavity B is used to measure the detection parameter B.

[0071] When analyzing formed elements using microscopic imaging, a single layer of formed elements must be tiled for effective identification and counting. Figure 1 and Figure 4 ,and Figures 11 to 16 In the embodiment of the multi-parameter formed component detection device shown, as the height of the detection liquid holding cavity A increases, the transparent area at the top for microscopic imaging observation remains the same, and the corresponding detection liquid volume increases, and the spread of formed components in the detection liquid holding cavity increases. Figure 4 As shown, multiple detection liquid holding cavities at different heights are provided. After the same detection liquid sample is injected into each of the connected detection liquid holding cavities at different heights, different tangible component spreading states will be formed at the bottom of the detection liquid holding cavities at different heights. Flexible selection and combination can be performed based on the quantitative characteristics of different tangible components in unit volume until the optimal photographing area combination is found.

[0072] For example, when analyzing and calculating a certain visible component A, i.e., test parameter A, microscopic imaging of the visible component within first test liquid holding cavity A501 is performed, and S1 microscopic images are obtained for use in detecting parameter A. If both the quantity and proportion of the visible component in the microscopic image meet the requirements for subsequent analysis and calculation, the corresponding microscopic imaging process for detection parameter A is complete. Assuming that the typical concentration range of detection parameter B is 1 / N of the typical concentration range of detection parameter A, then when analyzing and calculating another visible component B, i.e., detection parameter B, only microscopic imaging of first test liquid holding cavity A501 would require N times the number of images required, i.e., S1 × N microscopic images would be required to obtain a sufficient sample volume. If the height of the second detection liquid holding chamber A502 is M times that of the first detection liquid holding chamber A501, then, given the same detection liquid holding chamber cross-sectional area, the volume of the sample in the second detection liquid holding chamber A502 becomes M times that of the first detection liquid holding chamber A501. At this point, given the same microscopic image area, the concentration or distribution density of the formed component B will also increase by M times. In this case, only S1×N / M microscopic images are needed to obtain a sufficient sample volume for analysis of the formed component B. Therefore, the number of microscopic images acquired can be greatly reduced, improving imaging efficiency.

[0073] like Figure 1 and Figure 4 as well as Figure 16 As shown, in an embodiment of a multi-parameter formed element detection device, five parallel test fluid holding chambers are provided. The number of parallel test fluid holding chambers can be two or more; the specific number can be flexibly set based on the components of the formed element being detected and the number of detection parameters. For general blood testing, at least two different test fluid holding chambers are provided. One test fluid holding chamber is used for red blood cell and platelet analysis, and the other test fluid holding chamber is used for white blood cell analysis. Both test fluid holding chambers can be filled with blood samples of the same dilution factor. The different test fluid holding chambers can be connected in series or in parallel, and sample volume control can be achieved as long as their heights are different. In some embodiments, when the image size is used to determine the area of ​​the imaging test fluid, the different test fluid holding chambers can have the same cross-sectional area, but only different heights.

[0074] like Figure 1 and Figure 4 as well as Figure 16As shown, in an embodiment of a multi-parameter formed component detection device, five parallel detection liquid holding chambers are provided, namely a first detection liquid holding chamber A501, a second detection liquid holding chamber A502, a third detection liquid holding chamber A503, a fourth detection liquid holding chamber A504, and a fifth detection liquid holding chamber A505. The heights of the five parallel detection liquid holding chambers can be distributed in an equidistant manner or in an unequal manner. The specific height difference can be flexibly set according to the concentration span between different detection parameters.

[0075] In some embodiments of a multi-parameter formed element detection device, detection parameter A is used to detect white blood cells in a living organism's blood; detection parameter B is used to detect red blood cells in a living organism's blood; or detection parameter B is used to detect platelets in a living organism's blood. The combination of detection parameters A and B can be flexibly selected based on the actual test sample. The height of the corresponding test fluid chamber can be adaptively adjusted based on different detection parameter combinations.

[0076] In other embodiments of the multi-parameter formed element detection device, detection parameter A is for detecting worm eggs in a biological feces suspension; detection parameter B is for detecting bacteria in a biological feces suspension; and detection parameter B is for detecting cells in a biological feces suspension.

[0077] In some embodiments of the multi-parameter formed element detection device, detection parameter A is for detecting casts in biological urine; detection parameter A is for detecting crystals in biological urine; detection parameter B is for detecting cells in biological urine; and detection parameter B is for detecting bacteria in biological urine.

[0078] Specific detection parameters can be set according to the type of sample actually being detected and the specific conditions of the tangible components of the target to be detected, that is, the target object to be detected.

[0079] like Figure 1 and Figure 4 ,and Figures 11 to 16 In an embodiment of a multi-parameter formed element detection device, as shown, some formed elements within the fourth and fifth test liquid holding chambers A504 and A505 may overlap. This image is not suitable for analyzing these overlapping formed elements, but is suitable for analyzing other formed elements at lower concentrations. If multiple layers of target objects are found in test liquid holding chamber A, detection of the target objects is switched to test liquid holding chamber B.

[0080] like Figure 1 and Figure 4 ,and Figures 11 to 16In the embodiment of the multi-parameter formed component detection device shown, if the target object is not detected in detection liquid chamber B, detection of the target object is performed in detection liquid chamber A. Both detection liquid chambers A and B can be used to analyze the same parameter, i.e., the same formed component. They can also be used to analyze different parameters, i.e., different formed components. The two chambers can be flexibly switched between them based on actual needs.

[0081] In other embodiments of the multi-parameter formed component detection device, if no target object is found in the detection liquid containing cavity A or the number of the target object is small, the detection of the target object is performed in the detection liquid containing cavity B.

[0082] In some embodiments of the multi-parameter formed element detection device, the target to be detected is red blood cells in the blood of an organism; the target to be detected is white blood cells in the blood of an organism; and the target to be detected is platelets in the blood of an organism.

[0083] In some embodiments of the multi-parameter formed element detection device, the target to be detected is insect eggs in the biological feces suspension; the target to be detected is bacteria in the biological feces suspension; the target to be detected is cells in the biological feces suspension.

[0084] In some embodiments of the multi-parameter formed element detection device, the target to be detected is cells in biological urine; the target to be detected is bacteria in biological urine; the target to be detected is tubular objects in biological urine; the target to be detected is crystals in biological urine.

[0085] like Figures 1 to 15 As shown, the above description is only an embodiment of the present application and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the application description and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A multi-parameter tangible component detection chip, characterized by: Used for the detection of formed elements in biological blood, urine, feces or body fluids, including a detection liquid holding cavity A, a detection liquid holding cavity B, and a sample addition port; The sample adding port is used to add the sample to be tested; The sample addition port is connected to the detection liquid holding cavity A; The sample addition port is connected to the detection liquid holding cavity B; The first detection channel includes a detection liquid holding cavity A, and the first detection channel is provided with a first detection channel sample port and a first detection channel exhaust port; The sample addition port of the first detection channel is connected to the detection liquid holding cavity A; the exhaust port of the first detection channel is also connected to the detection liquid holding cavity A; The second detection channel includes a detection liquid containing cavity B, and the second detection channel is correspondingly provided with a second detection channel sample port and a second detection channel exhaust port; The sample injection port of the second detection channel is connected to the detection liquid holding chamber B; the exhaust port of the second detection channel is also connected to the detection liquid holding chamber B; the first detection channel and the second detection channel are independent of each other and are not connected to each other; The liquid holding height of the detection liquid holding cavity A is higher than the liquid holding height of the detection liquid holding cavity B; At least one of the upper part or the lower part of the detection liquid containing cavity A is made of a transparent material; At least one of the upper portion or the lower portion of the detection liquid containing cavity B is made of a transparent material; The detection liquid holding cavity A is used for measuring the detection parameter A; The detection liquid holding cavity B is used for measuring the detection parameter B.

2. The multi-parameter tangible component detection chip according to claim 1, characterized in that: Including any one of the following technical features: Feature T10, the detection liquid holding chamber A and the detection liquid holding chamber B have bottoms at the same height; Feature T20: The detection liquid containing cavity A and the detection liquid containing cavity B have tops at the same height.

3. The multi-parameter tangible component detection chip according to claim 1, characterized in that: Including any one of the following technical features: Feature TA10, the detection fluid holding chamber A is used to detect white blood cells in the blood of an organism; Feature TA20, the detection fluid holding chamber B is used to detect red blood cells in the blood of an organism; Feature TA30, the detection liquid containing chamber B is used to detect platelets in the blood of a biological being; Feature TB10, the detection liquid holding chamber A is used to detect insect eggs in a biological fecal suspension; Feature TB20, the test liquid holding chamber B is used to detect bacteria in biological fecal suspension; Feature TB30, the test liquid holding chamber B is used to detect cells in a biological fecal suspension; Feature TC10, the test liquid holding chamber A is used to detect tubular objects in biological urine; Feature TC20, the test fluid holding chamber A is used to detect crystals in biological urine; Feature TC30, the detection liquid holding chamber B is used to detect cells in biological urine; Feature TC40, the detection liquid containing chamber B is used to detect bacteria in biological urine.

4. The multi-parameter tangible component detection chip according to claim 1, characterized in that: The detection liquid holding cavity A or the detection liquid holding cavity B is formed by the bottom plate and the upper plate, and the detection liquid holding cavity A or the detection liquid holding cavity B is formed by the concave portion of the upper plate; or the detection liquid holding cavity A or the detection liquid holding cavity B is formed by the concave portion of the lower plate; The bottom plate and the upper plate are welded, bonded or ultrasonically welded into one; It also includes an exhaust port; the exhaust port is connected to the detection liquid containing cavity A; the exhaust port is connected to the detection liquid containing cavity B; and the exhaust port is connected to the external atmosphere.

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