Cell biological power supply and preparation process thereof

Abstract

The invention discloses a cell biological power supply and a preparation process thereof, and belongs to the technical field of biological power generation, the cell biological power supply selects clone cells of specific electric cells in an electric eel body as a biological power supply, the selected electric eel cells are dissimilatory cells on two sides of the electric eel body, and the cell biological power supply is a cell biological power supply. A plurality of refined cells are combined for use to form a power supply capable of outputting high-voltage electricity; in the selected electric eel cells, two sides of a cell membrane structure are in an asymmetric form; in a single electric eel cell, a plurality of electric eel cells form cell ordered structures, a plurality of cell ordered structures form connective tissues, a plurality of connective tissues form power generation organs, and a plurality of power generation organs are combined to form a cell biological power supply. The problem of emission pollution during discharging can be effectively avoided, damage to the environment around the power supply is further avoided, the practicability of the biological power supply is improved, and the execution efficiency of the power supply during power storage or discharging is effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of bio-power generation, and more particularly to a cell bio-power source and a preparation process thereof. Background Art

[0002] Most existing power sources are in the form of batteries. Whether they are lead-acid batteries, lithium batteries, or liquid batteries, they all need to be made into a large fixed shape. Various batteries have different advantages and disadvantages in terms of raw materials, discharge properties, lifespan, emissions, etc. The birth and application of power sources that store electrical energy in biological cells (hereinafter referred to as biopower sources) can solve many of the above problems.

[0003] Based on the above, the inventors found that:

[0004] In the actual use of conventional power supply,

[0005] ① In the normal use of power supply, the power supply may emit some substances before and after use, and these substances may cause a certain degree of damage to the surrounding environment;

[0006] ② Traditional power supplies require manual operation of switches during discharge to control the process of storing or discharging electricity, which reduces the efficiency of the power supply during storage or discharge.

[0007] Therefore, in view of this, the existing structure is studied and improved, and a cell biological power source and its preparation process are provided, in order to achieve a purpose with greater practical value. Summary of the Invention

[0008] 1. Technical problems to be solved

[0009] In response to the problems existing in the prior art, the purpose of the present invention is to provide a cellular biopower source and its preparation process, which can effectively avoid the problem of emission pollution during discharge, thereby avoiding damage to the environment surrounding the power source, improving the practicality of the biopower source, and effectively improving the power source's execution efficiency during storage or discharge.

[0010] 2. Technical solution

[0011] To solve the above problems, the present invention adopts the following technical solutions.

[0012] A cellular biopower source, wherein the cellular biopower source uses cloned cells of specialized electric cells in the electric eel as the biopower source;

[0013] The electric eel cells used are the alienated cells on both sides of the electric eel's body. Multiple refined cells are used in combination to form a power source that can output high-voltage electricity.

[0014] In the selected electric eel cells, the cell membrane structure is asymmetrical on both sides;

[0015] In a single electric eel cell, the voltage between the front and back membranes ranges from 50 to 150 mV;

[0016] The ordered cell structure is formed by multiple electric eel cells, and the power generation voltage of a single ordered cell structure ranges from 0.8 to 2.5V;

[0017] Connective tissue is formed by an orderly structure of multiple cells, and the power generation voltage of a single connective tissue ranges from 20 to 42V;

[0018] The power generating organs are formed by multiple connective tissues, and the power generation voltage of a single power generating organ ranges from 150 to 320V;

[0019] The cellular biological power source is formed by the combination of multiple power generation organs.

[0020] Furthermore, both sides of the cell membrane structure are processed by sodium ions, closing the voltage-gated sodium ion channels on the front side of the cell, forming an asymmetric structure of the cell membrane.

[0021] Furthermore, the connective tissue is stimulated to generate electricity, and the stimulation methods include:

[0022] Mechanical stimulation, specifically: stretching or compressing collagen fibers in connective tissue, then generating a transient voltage of 10mV based on the piezoelectric effect;

[0023] Chemical stimulation, specifically: local pH regulation changes in connective tissue, activation of fibroblast membrane proton channels, H + Directed movement, forming an electric current;

[0024] Thermal stimulation specifically involves setting a temperature gradient to force ion migration in the tissue, forming a thermoelectric effect, and ensuring a temperature difference of no less than 5°C during thermal stimulation.

[0025] Furthermore, during thermal stimulation, Fe3O4 nanoparticles are injected into the connective tissue to form an alternating magnetic field, which then generates local heating and triggers the thermoelectric effect.

[0026] Furthermore, after the cell bio-power source is formed, a cultivation container for the bio-power source is established, and an excitation signal device is set on the cultivation container. The excitation signal device includes:

[0027] Chemical signal stimulator: Chemical signal stimulator precisely delivers ATP / K through microfluidic chip + , and form simulated neural signals, or generate H through glucose oxidase reaction + , periodically changing pH, or releasing cAMP through light-sensitive proteins, converting light-controlled signals into chemical signals;

[0028] Physical field exciter: The physical field exciter uses focused sound pressure to deform piezoelectric collagen and generate electricity, or uses an alternating magnetic field to cause eddy currents in nanoparticles to generate heat and trigger the thermoelectric effect; or uses near-infrared light to heat photothermal materials and form ion gradient power generation.

[0029] Furthermore, by focusing the sound pressure to cause the piezoelectric collagen to deform and generate electricity, the penetration depth is 8cm with an accuracy of ±2mm;

[0030] When the alternating magnetic field causes the nanoparticles to generate eddy currents and trigger the thermoelectric effect, the penetration depth is full organ penetration with an accuracy of ±5mm.

[0031] When near-infrared light is used to heat up the photothermal material and form ion gradient power generation, its penetration depth is 4cm and its accuracy is ±0.1mm.

[0032] A preparation process of a cell-based bio-power source, the specific implementation steps of the preparation process of the cell-based bio-power source are as follows:

[0033] Step 1: Selection of electric eel cells: Select the alienated cells on both sides of the electric eel's body, extract the electric eel's discharge cells, and process the electric eel's discharge cells. At this time, the voltage-gated sodium ion channels on the front side of the cells are closed, so that the electric eel's cell membrane meets the standard requirements;

[0034] Step 2: Cloning and cultivation of electric eel cells: Select electric eel cells that meet the standard requirements, add nutrient solution to the culture container, culture it in a controlled temperature environment of 15°C, add growth hormone, and continuously pass oxygen-rich air to form a suspension. The number of cells in the suspension should be ≥ (0.4-0.45) × 10 2 / ml, complete cell cloning culture;

[0035] Step 3: Formation of cell structure: Raise the temperature of the cloned cells to 60°C and maintain for 2 hours. Then, centrifuge the temperature-controlled cell group and clone and culture again. After culture, screen and select cells with a cell number of ≥ (0.4-0.45) × 10 2 / ml culture population, added to muscle cells, and then the cloned cells and muscle cells were connected in series to form an ordered cell structure. The power generation voltage of the ordered cell structure was 0.8 to 2.5V;

[0036] Step 4: Formation of the electric organ: Cultivate connective tissue and stimulate it to generate electricity. The voltage of a single connective tissue is 20 to 42V. Then, add cell structures to the connective tissue and adjust the position of the cell structures so that the connective tissue wraps around the cell structures into rows. Then, connect them in parallel to form the electric organ. The voltage of a single electric organ is 150 to 320V.

[0037] Step 5: Formation of a bio-power source: Orderly combine multiple electric organs to form a bio-power source, add culture medium to the bio-power source, activate the cells, and complete the self-growth and replication of the cells in the bio-power source. Then, build a cultivation container for the bio-power source and set an excitation signal on the cultivation container to complete the excitation discharge of the bio-power source.

[0038] Furthermore, in step five, when the cells in the bio-power source grow and replicate on their own, the replication time is 20 to 36 hours, the maximum number of replication generations does not exceed 15 generations, and the division stops after the monolayer converges.

[0039] 3. Beneficial effects

[0040] Compared with the prior art, the advantages of the present invention are:

[0041] ① This scheme uses the power generation properties of biological cells to prepare a bio-power source. This bio-power source can effectively avoid the problem of emission pollution during discharge, thereby avoiding damage to the surrounding environment of the power source. At the same time, by controlling the movement of biological cells, the discharge time and amount of power of the bio-power source can be controlled.

[0042] At the same time, bio-power has the characteristics of small size, high density and large energy. At the same time, the power generation, storage and discharge of bio-power are all based on ions. Under the same volume, the ion density is high, which ensures the storage of a large amount of electrical energy. In addition, the actual form of bio-power is plastic. In actual use, it can be made into various shapes according to needs and applied to different occasions. In summary, this solution improves the practicality of bio-power.

[0043] ② In this solution, the bio-power source uses brain waves to send instructions to control the storage and discharge of electricity. When the brain neurons send out a storage or discharge signal, the bio-power source cells quickly change their arrangement to achieve the storage or discharge process, eliminating the need for manual operations such as pressing switches. Furthermore, due to the extremely high transmission rate of brain waves, the power source's efficiency in storage or discharge can be effectively improved.

[0044] ③ In this solution, during the use of the bio-power source, the power source can be installed outside or inside the tool according to the type of tool used, which improves the convenience of power source installation. At the same time, the bio-power source will not explode, thereby improving the safety of power source use. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 A schematic diagram of the process for preparing the bio-power source of the present invention;

[0046] Figure 2 A schematic diagram of the process of selecting electric eel cells for preparing the bio-power source of the present invention;

[0047] Figure 3 A schematic diagram of the process of cloning and cultivating electric eel cells prepared as the biopower source of the present invention;

[0048] Figure 4 A schematic diagram of the process for forming a cell structure prepared by the bio-power source of the present invention;

[0049] Figure 5 Schematic diagram of the process of forming an electric organ prepared for the bio-power source of the present invention and forming a bio-power source. DETAILED DESCRIPTION

[0050] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention; it is obvious that the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0051] Example 1:

[0052] A cellular biopower source, wherein the cellular biopower source uses cloned cells of specialized electric cells in the electric eel as the biopower source;

[0053] The electric eel cells used are the alienated cells on both sides of the electric eel's body. Multiple refined cells are used in combination to form a power source that can output high-voltage electricity.

[0054] In the selected electric eel cells, the cell membrane structure is asymmetrical on both sides;

[0055] In a single electric eel cell, the voltage between the front and back membranes ranges from 50 to 150 mV;

[0056] The ordered cell structure is formed by multiple electric eel cells, and the power generation voltage of a single ordered cell structure ranges from 0.8 to 2.5V;

[0057] Connective tissue is formed by an orderly structure of multiple cells, and the power generation voltage of a single connective tissue ranges from 20 to 42V;

[0058] The power generating organs are formed by multiple connective tissues, and the power generation voltage of a single power generating organ ranges from 150 to 320V;

[0059] The cellular biological power source is formed by the combination of multiple power generation organs.

[0060] Specifically, both sides of the cell membrane structure are processed by sodium ions, closing the voltage-gated sodium ion channels on the front side of the cell, forming an asymmetric structure of the cell membrane.

[0061] This allows the action potentials to not cancel each other out at the scale of the entire cell when the cell discharges.

[0062] Specifically, the connective tissue is stimulated to generate electricity, and the stimulation methods include:

[0063] Mechanical stimulation, specifically: stretching or compressing collagen fibers in connective tissue, then generating a transient voltage of 10mV based on the piezoelectric effect;

[0064] Chemical stimulation, specifically: local pH regulation changes in connective tissue, activation of fibroblast membrane proton channels, H + Directed movement, forming an electric current;

[0065] Thermal stimulation specifically involves setting a temperature gradient to force ion migration in the tissue, forming a thermoelectric effect, and ensuring a temperature difference of no less than 5°C during thermal stimulation.

[0066] According to needs, choose the appropriate stimulation method to ensure that the power generation effect of the connective tissue meets the requirements.

[0067] Specifically, during thermal stimulation, Fe3O4 nanoparticles are injected into the connective tissue to form an alternating magnetic field, which then generates local heating and triggers the thermoelectric effect.

[0068] Specifically, after the cell bio-power source is formed, a cultivation container for the bio-power source is established, and an excitation signal device is set on the cultivation container. The excitation signal device includes:

[0069] Chemical signal stimulator: Chemical signal stimulator precisely delivers ATP / K through microfluidic chip + , and form simulated neural signals, or generate H through glucose oxidase reaction + , periodically changing pH, or releasing cAMP through light-sensitive proteins, converting light-controlled signals into chemical signals;

[0070] Physical field exciter: The physical field exciter uses focused sound pressure to deform piezoelectric collagen and generate electricity, or uses an alternating magnetic field to cause eddy currents in nanoparticles to generate heat and trigger the thermoelectric effect; or uses near-infrared light to heat photothermal materials and form ion gradient power generation.

[0071] Specifically, by focusing sound pressure to cause the piezoelectric collagen to deform and generate electricity, its penetration depth is 8cm and its accuracy is ±2mm;

[0072] When the alternating magnetic field causes the nanoparticles to generate eddy currents and trigger the thermoelectric effect, the penetration depth is full organ penetration with an accuracy of ±5mm.

[0073] When near-infrared light is used to heat up the photothermal material and form ion gradient power generation, its penetration depth is 4cm and its accuracy is ±0.1mm.

[0074] According to the power generation needs of the bio-power source, the corresponding excitation signal device is selected to ensure that the power generation results meet the power generation requirements.

[0075] Example 2:

[0076] Further description is given with reference to the above-mentioned embodiment 1.

[0077] See also Figure 1-Figure 5 A preparation process of a cell bio-power source. The specific implementation steps of the preparation process of the cell bio-power source are as follows:

[0078] Step 1: Selection of electric eel cells: Select the alienated cells on both sides of the electric eel's body, extract the electric eel's discharge cells, and process the electric eel's discharge cells. At this time, the voltage-gated sodium ion channels on the front side of the cells are closed, so that the electric eel's cell membrane meets the standard requirements;

[0079] Step 2: Cloning and cultivation of electric eel cells: Select electric eel cells that meet the standard requirements, add nutrient solution to the culture container, culture it in a controlled temperature environment of 15°C, add growth hormone, and continuously pass oxygen-rich air to form a suspension. The number of cells in the suspension should be ≥ (0.4-0.45) × 10 2 / ml, complete cell cloning culture;

[0080] Step 3: Formation of cell structure: Raise the temperature of the cloned cells to 60°C and maintain for 2 hours. Then, centrifuge the temperature-controlled cell group and clone and culture again. After culture, screen and select cells with a cell number of ≥ (0.4-0.45) × 10 2 / ml culture population, added to muscle cells, and then the cloned cells and muscle cells were connected in series to form an ordered cell structure. The power generation voltage of the ordered cell structure was 0.8 to 2.5V;

[0081] Step 4: Formation of the electric organ: Cultivate connective tissue and stimulate it to generate electricity. The voltage of a single connective tissue is 20 to 42V. Then, add cell structures to the connective tissue and adjust the position of the cell structures so that the connective tissue wraps around the cell structures into rows. Then, connect them in parallel to form the electric organ. The voltage of a single electric organ is 150 to 320V.

[0082] Step 5: Formation of a bio-power source: Orderly combine multiple electric organs to form a bio-power source, add culture medium to the bio-power source, activate the cells, and complete the self-growth and replication of the cells in the bio-power source. Then, build a cultivation container for the bio-power source and set an excitation signal on the cultivation container to complete the excitation discharge of the bio-power source.

[0083] Specifically, when cells in the biopower source grow and replicate on their own, the replication time is 20 to 36 hours, the maximum number of replication generations does not exceed 15 generations, and the division stops after the monolayer converges.

[0084] In this way, the cells can be synchronized with the host tissue, the risk of cell aging or cancer can be avoided, and excessive cell proliferation can be prevented.

[0085] The above description is merely a preferred embodiment of the present invention; however, the scope of protection of the present invention is not limited thereto. Any person skilled in the art who, within the technical scope disclosed by the present invention, makes equivalent substitutions or modifications based on the technical solutions and improved concepts of the present invention shall be covered by the scope of protection of the present invention.

Claims

1. A cellular biopower source, characterized by: The cell biological power source uses cloned cells of specialized electric cells in the electric eel as the biological power source; The electric eel cells used are the alienated cells on both sides of the electric eel's body. Multiple refined cells are used in combination to form a power source that can output high-voltage electricity. In the selected electric eel cells, the cell membrane structure is asymmetrical on both sides; In a single electric eel cell, the voltage between the front and back membranes ranges from 50 to 150 mV; The ordered cell structure is formed by multiple electric eel cells, and the power generation voltage of a single ordered cell structure ranges from 0.8 to 2.5V; Connective tissue is formed by an orderly structure of multiple cells, and the power generation voltage of a single connective tissue ranges from 20 to 42V; The power generating organs are formed by multiple connective tissues, and the power generation voltage of a single power generating organ ranges from 150 to 320V; The cellular biological power source is formed by the combination of multiple power generation organs.

2. The cell biopower source according to claim 1, characterized in that: Both sides of the cell membrane structure are processed by sodium ions, closing the voltage-gated sodium ion channels on the front side of the cell, forming an asymmetric structure of the cell membrane.

3. The cellular bio-power source according to claim 1, characterized in that: Stimulates the connective tissue to generate electricity, including: Mechanical stimulation, specifically: stretching or compressing collagen fibers in connective tissue, then generating a transient voltage of 10mV based on the piezoelectric effect; Chemical stimulation, specifically: local pH regulation changes in connective tissue, activation of fibroblast membrane proton channels, H + Directed movement, forming an electric current; Thermal stimulation specifically involves setting a temperature gradient to force ion migration in the tissue, forming a thermoelectric effect, and ensuring a temperature difference of no less than 5°C during thermal stimulation.

4. The cellular biopower source according to claim 3, characterized in that: During thermal stimulation, Fe3O4 nanoparticles are injected into the connective tissue to form an alternating magnetic field, which then generates local heating and triggers the thermoelectric effect.

5. The cellular bio-power source according to claim 1, characterized in that: After the cell bio-power source is formed, a cultivation container for the bio-power source is established, and an excitation signal device is set on the cultivation container. The excitation signal device includes: Chemical signal stimulator: Chemical signal stimulator precisely delivers ATP / K through microfluidic chip + , and form simulated neural signals, or generate H through glucose oxidase reaction + , periodically changing pH, or releasing cAMP through light-sensitive proteins, converting light-controlled signals into chemical signals; Physical field exciter: The physical field exciter uses focused sound pressure to deform piezoelectric collagen and generate electricity, or uses an alternating magnetic field to cause eddy currents in nanoparticles to generate heat and trigger the thermoelectric effect; or uses near-infrared light to heat photothermal materials and form ion gradient power generation.

6. The cellular bio-power source according to claim 5, characterized in that: By focusing the sound pressure to cause the piezoelectric collagen to deform and generate electricity, the penetration depth is 8cm with an accuracy of ±2mm. Through the alternating magnetic field, the nanoparticles are heated by eddy currents, triggering the thermoelectric effect. The penetration depth is full organ penetration with an accuracy of ±5mm. When near-infrared light is used to heat up the photothermal material and form ion gradient power generation, its penetration depth is 4cm and its accuracy is ±0.1mm.

7. A process for preparing a cellular bio-power source according to any one or more of claims 1 to 6, characterized in that: The specific implementation steps of the preparation process of the cell biopower source are as follows: Step 1: Selection of electric eel cells: Select the alienated cells on both sides of the electric eel's body, extract the electric eel's discharge cells, and process the electric eel's discharge cells. At this time, the voltage-gated sodium ion channels on the front side of the cells are closed, so that the electric eel's cell membrane meets the standard requirements; Step 2: Cloning and cultivation of electric eel cells: Select electric eel cells that meet the standard requirements, add nutrient solution to the culture container, culture it in a controlled temperature environment of 15°C, add growth hormone, and continuously pass oxygen-rich air to form a suspension. The number of cells in the suspension should be ≥ (0.4-0.45) × 10 2 / ml, complete cell cloning culture; Step 3: Formation of cell structure: Raise the temperature of the cloned cells to 60°C and maintain for 2 hours. Then, centrifuge the temperature-controlled cell group and clone and culture again. After culture, screen and select cells with a cell number of ≥ (0.4-0.45) × 10 2 / ml culture population, added to muscle cells, and then the cloned cells and muscle cells were connected in series to form an ordered cell structure. The power generation voltage of the ordered cell structure was 0.8 to 2.5V; Step 4: Formation of the electric organ: Cultivate connective tissue and stimulate it to generate electricity. The voltage of a single connective tissue is 20 to 42V. Then, add cell structures to the connective tissue and adjust the position of the cell structures so that the connective tissue wraps around the cell structures into rows. Then, connect them in parallel to form the electric organ. The voltage of a single electric organ is 150 to 320V. Step 5: Formation of a bio-power source: Orderly combine multiple electric organs to form a bio-power source, add culture medium to the bio-power source, activate the cells, and complete the self-growth and replication of the cells in the bio-power source. Then, build a cultivation container for the bio-power source and set an excitation signal on the cultivation container to complete the excitation discharge of the bio-power source.

8. The process for preparing a cellular bio-power source according to claim 7, characterized in that: In the step 5, when the cells in the bio-power source grow and replicate on their own, the replication time is 20 to 36 hours, the maximum number of replication generations does not exceed 15 generations, and the division stops after the monolayer converges.

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