Method for cultivating agaricus bisporus by using liquid strain clinker

Abstract

The invention discloses a method for cultivating agaricus bisporus through liquid strain clinker, and belongs to the technical field of edible fungus cultivation. The agaricus bisporus cultivation method provided by the invention comprises the following steps: sequentially carrying out shake-flask culture, liquid fermentation tank culture, cultivation culture, soil covering, fruiting management and harvesting on agaricus bisporus strains, wherein the cultivation is carried out on the prepared clinker cultivation material. A cultivation experiment result shows that compared with a traditional agaricus bisporus cultivation process, the cultivation method has obvious advantages in the aspects of strain preparation time, cultivation material preparation and bacteria running time, unit area yield and the like; and moreover, the production process of the agaricus bisporus is simplified, a tedious fermentation process is reduced, the probability of strain pollution is reduced, and the initial investment cost and the production cost are effectively reduced. The invention provides a new method for efficient production of agaricus bisporus, and has outstanding practical application value and wide application prospect.

Description

Technical Field

[0001] The invention relates to the technical field of edible fungus cultivation, in particular to a method for cultivating Agaricus bisporus with liquid spawn cooked material. Background Art

[0002] Agaricus bisporus, also known as white mushroom and button mushroom, is the world's most produced edible mushroom variety. Its delicious meat, tender texture, high protein and low fat nutritional characteristics are in line with modern healthy consumption trends, and can enhance immune function and human metabolic function, making it deeply loved by consumers.

[0003] The Agaricus bisporus industry holds a significant position in the global agricultural landscape. While China currently cultivates a large volume of the mushroom, its independent innovation capabilities in key areas, including cultivation techniques, strain technology, and industrial equipment, are relatively low, resulting in a Western technology monopoly. Regarding cultivation techniques, domestic production processes are primarily imported from Western countries, resulting in significant technological gaps and disparities in yields. Regarding strains, European and American companies have monopolized high-yielding strains through molecular marker breeding. Domestic companies using these strains must pay high annual patent fees. Regarding equipment, due to the high technical deviations among domestic manufacturers, the intelligent equipment used in industrial cultivation primarily comes from countries like the Netherlands, necessitating high-cost foreign investment. Traditional Agaricus bisporus cultivation relies heavily on Western technology, severely restricting production and directly impacting the industry's development.

[0004] Traditional solid culture fermentation materials are used to cultivate Agaricus bisporus. The preparation process of solid culture materials, from mother culture, original culture, to cultivated culture, is relatively complicated, which is mainly manifested in the long culture time, unstable quality, low yield, etc. of solid culture materials. In addition, high-yield strains are controlled by Western mushroom companies, and it is expensive to choose to purchase strains. In terms of culture material technology, traditional fermentation cultivation requires the pre-wetting of raw materials, pile building, primary fermentation, secondary fermentation, and tertiary fermentation. The process is cumbersome, difficult to control, and takes 40-45 days. The production efficiency is extremely low and the investment cost is high. Therefore, there is an urgent need to develop a method that can replace the traditional solid culture fermentation materials for cultivating Agaricus bisporus in large quantities or even completely. Summary of the Invention

[0005] The purpose of the present invention is to provide a method for cultivating Agaricus bisporus using liquid spawn and cooked material to solve the problems existing in the above-mentioned prior art. The Agaricus bisporus cultivation method provided by the present invention can replace traditional Agaricus bisporus cultivation, provide a new technical process for Agaricus bisporus cultivation, solve the problems of the existing single cultivation model, large investment in machinery and equipment, and high cultivation threshold, and realize controllable, stable, and low-cost factory production of Agaricus bisporus.

[0006] To achieve the above object, the present invention provides the following solutions:

[0007] The present invention provides a method for cultivating Agaricus bisporus using liquid spawn cooked material, comprising the following steps:

[0008] inoculating Agaricus bisporus into a shake flask culture medium, performing shake flask culture to obtain a shake flask strain, transferring the shake flask strain into a fermentation medium, performing fermentation culture to obtain a liquid strain;

[0009] The liquid fungus is inoculated into the cultivation material and cultivated. After the cultivation is completed, the material is dug, soil is covered, the mushrooms are managed and harvested.

[0010] Furthermore, the raw materials of the shake flask culture medium include: 200g potatoes, 20g glucose, 2g peptone, 2g soy flour, 1g potassium dihydrogen phosphate, 0.5g magnesium sulfate, 0.5g sodium chloride, 1L purified water, and 0.1mL defoaming agent.

[0011] Furthermore, the shake flask culture temperature is 23-28° C., the rotation speed is 140-160 rpm, and the time is 6-9 days.

[0012] Furthermore, the raw materials of the fermentation medium include: 21 kg of white sugar, 1.4 kg of corn flour, 1.4 kg of peptone, 700 g of potassium dihydrogen phosphate, 700 g of magnesium sulfate, 350 g of sodium chloride, 700 L of purified water and 80 mL of defoaming agent.

[0013] Furthermore, the fermentation culture temperature is 23-28° C., the ventilation pressure is 0.1-0.15 MPa, and the fermentation time is 6-9 days.

[0014] Furthermore, the raw materials of the cultivation material include: wood-decaying fungus residue, agricultural waste, gypsum, lime, ammonium sulfate and water.

[0015] Furthermore, the wood-rotting fungus residue is enoki mushroom residue or king oyster mushroom residue; and the agricultural waste is livestock manure or crop by-products.

[0016] Furthermore, the preparation of the planting material comprises the following steps:

[0017] The raw materials are mixed and stirred, and packaged into 5 kg packages per pot, with 15-25 holes punched in each pot, sterilized, and cooled.

[0018] Furthermore, the inoculation amount of the liquid bacteria is 1% by mass.

[0019] Furthermore, the cultivation temperature is 23-28° C., the humidity is 50%-60%, and the cultivation time is 17-21 days.

[0020] The present invention discloses the following technical effects:

[0021] The liquid spawn clinker cultivation technology of the present invention can completely replace the traditional grain spawn fermentation material cultivation of Agaricus bisporus, achieving a major breakthrough: significantly reducing the cultivation cost of Agaricus bisporus; greatly shortening the cultivation cycle, building a new and efficient cultivation model; enhancing the stability of the cultivation substrate ratio and improving production efficiency. The promotion and application of this technology will promote the upgrade of the Agaricus bisporus industry towards controllable, stable and environmentally friendly. Through the standardized clinker cultivation process, the time and cost bottlenecks of the traditional fermentation model are broken through, and the unit output efficiency is significantly improved; the technical model is highly consistent with the national green agricultural development requirements, and deeply coordinated with the current upgrade direction of the Agaricus bisporus industry towards intelligent, mechanized and intensive development; compared with traditional fermentation cultivation, it is more conducive to the industry to achieve a dual increase in output value and benefits, providing a core driving force for industrial innovation and development.

[0022] Compared with the traditional technology of cultivating Agaricus bisporus with fermented grain spawn, the liquid spawn clinker cultivation of Agaricus bisporus provided by the present invention has better production effect. The innovative establishment of a standardized process based on deep fermentation of liquid spawn reduces the spawn production cost by 35-45%; the clinker cultivation technology of Agaricus bisporus is used to shorten the cultivation material preparation and spawning stage from 40-45 days of traditional fermentation cultivation of Agaricus bisporus to 19-26 days; the development of a sterilization cultivation mode increases the utilization rate of the cultivation substrate by 30%, avoiding the discharge of toxic gases such as ammonia into the air; the raw materials use mushroom residue and decomposed straw, which can achieve 100% recycling of cultivation waste. The present invention simplifies the cultivation process of Agaricus bisporus. Its intelligent environmental control module, precise liquid spawn inoculation system and intensive production structure effectively promote the standardization, intelligence and cleanliness of Agaricus bisporus, improve the overall efficiency, and provide innovative solutions for the sustainable development of the Agaricus bisporus industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0024] Figure 1 Flow chart for the cultivation of Agaricus bisporus using liquid spawn;

[0025] Figure 2 These are the results of plate culture, shake flask culture and submerged fermentation in fermenter;

[0026] Figure 3 This is a picture of the culture medium made from King Oyster Mushroom residue as the main raw material;

[0027] Figure 4 This is a picture of the cultivation of liquid bacteria in a cultivation bottle or cultivation pot;

[0028] Figure 5 This is a diagram showing the fruiting of Agaricus bisporus using liquid spawn and cooked material. DETAILED DESCRIPTION

[0029] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0030] It should be understood that the terms described herein are intended only to describe particular embodiments and are not intended to limit the present invention. In addition, for numerical ranges herein, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. The intermediate value within any stated value or stated range, and each smaller range between any other stated value or intermediate value within the stated range, is also encompassed within the present invention. The upper and lower limits of these smaller ranges may be independently included or excluded within the scope.

[0031] Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein may also be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of any conflict with any incorporated document, the contents of this specification shall prevail.

[0032] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from the description of the invention. The description and examples are intended to be exemplary only.

[0033] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0034] The liquid spawn clinker cultivation of Agaricus bisporus provided by the present invention can achieve the effect of fully replacing the grain spawn fermentation material cultivation of Agaricus bisporus, can reduce the production cost of Agaricus bisporus, shorten the Agaricus bisporus cultivation cycle, provide another novel Agaricus bisporus cultivation mode, effectively improve the production efficiency of Agaricus bisporus, and increase the stability of the cultivation substrate ratio. The promotion and application of the present invention will be conducive to improving the production efficiency of Agaricus bisporus, promoting the production of Agaricus bisporus to be more controllable, more stable, and more environmentally friendly, and promoting the innovative development of the Agaricus bisporus industry. The technology and mode of cultivating Agaricus bisporus with liquid spawn clinker are more in line with the national standards for green agriculture and the intelligent, mechanized and intensive production of the current Agaricus bisporus industry. Compared with traditional fermentation cultivation, it is more conducive to the industry to achieve higher output value and benefits.

[0035] Example 1

[0036] 1. Shake flask strain preparation, inoculation and cultivation

[0037] a. Shake flask formulation and preparation

[0038] Formula: 200g potatoes, 20g glucose, 2g peptone, 2g soy flour, 1g potassium dihydrogen phosphate, 0.5g magnesium sulfate, 0.5g sodium chloride, 1L purified water, pH value natural, 0.1mL defoamer;

[0039] Preparation: Boil potatoes in water, filter, take the filtrate, and adjust the volume. Use 1L Erlenmeyer flasks and put 600mL of solution in each bottle. Weigh the remaining raw materials in the Erlenmeyer flasks directly without boiling. Add the rotor, plug with silicone stoppers, and cover with kraft paper and a mask.

[0040] b. Sterilization and cooling

[0041] 121℃, sterilize for 30min; after sterilization, wait until the temperature drops to 65℃ and place it on a clean bench to cool naturally to 23-28℃.

[0042] c. Vaccination

[0043] Select a plate that is already covered with mycelium, take the middle part of the concentric mycelium on the plate, remove the aerial mycelium, take a sample the size of a pea, and inoculate 20 pieces into each bottle.

[0044] d. Cultivation

[0045] Incubate in a shaker at 26°C and 150 rpm for 8 days. On the 6th day, break up the pellets with a stirrer. On the 8th day, transfer the mycelium to a fermenter when it reaches the specified concentration.

[0046] 2. Fermenter Preparation, Inoculation, and Cultivation

[0047] a. Fermentation tank formula and preparation

[0048] Formula: Select a fermentation tank with a capacity of 800L, 21kg of white sugar, 1.4kg of corn flour, 1.4kg of peptone, 700g of potassium dihydrogen phosphate, 700g of magnesium sulfate, 350g of sodium chloride, 700L of purified water, 80mL of defoamer, and natural pH;

[0049] Preparation: Weigh the medicine bottle and dissolve it in warm water in a bucket. Transfer it to the fermentation tank, add purified water and defoamer, and clamp the air inlet.

[0050] b. Sterilization and cooling

[0051] 121℃, sterilize for 120min; after sterilization, wait until the temperature drops to 65℃ and transfer to the clean cooling workshop for air connection and forced cooling, and wait until the temperature cools to 23-28℃.

[0052] c. Shake flask strain selection

[0053] Use odorless, pollution-free bacteria with a concentration of 145-155 CFU / mL and a culture time of 8 days.

[0054] d. Vaccination

[0055] Use a flame gun and alcohol wipe to burn the inoculation port. Light a flame ring at the inoculation port to briefly burn and disinfect the shake flask surface. Slowly pour the shake flask culture into the shake flask. Reserve 50mL to test the shake flask for contamination.

[0056] e. Cultivation

[0057] The fermentation tank culture workshop temperature was set at 26°C, the ventilation pressure was set at 0.1-0.15 MPa, and the culture was carried out for 8 days.

[0058] 3. Preparation of clinker cultivation materials, inoculation of liquid bacteria, and cultivation

[0059] a. Recipe

[0060] 50% King Oyster Mushroom Residue, 40% Cow Dung, 5% Gypsum, 3% Lime, 2% Ammonium Sulfate.

[0061] b. Preparation

[0062] Weigh the raw materials according to the ratio, mix gypsum, lime and ammonium sulfate first as the small ingredients; mix the oyster mushroom residue and cow dung in a horizontal mixer, then add the small ingredients and mix thoroughly; adjust the pH value to 7.0-7.5 and the water content to 65-70%.

[0063] c. Potting and drilling

[0064] Use 35cm×35cm×13cm high-pressure resistant plastic pots, put 5kg of planting material in each pot, make 25 holes in the planting material (specifications: 2cm diameter round holes, depth is the depth of the planting material), and put on air vent covers.

[0065] d. Sterilization and cooling

[0066] 121℃, sterilize for 120min; after sterilization, wait until the temperature drops to 65℃ and transfer to the clean cooling workshop for forced cooling until the temperature cools to 23-28℃.

[0067] e. Bacteria selection:

[0068] Select liquid fermentation tank strains that are odorless, pollution-free, have a concentration of 150-160 CFU / mL, and a culture time of 8 days.

[0069] f. Vaccination

[0070] In the clean inoculation workshop, use a metering pump to evenly spray the liquid bacteria on the surface of the cultivation material and in the perforated holes, with an inoculation amount of 1%.

[0071] g. Cultivation

[0072] The culture room was set at 26°C and 60% humidity and cultured in the dark for 21 days.

[0073] 4. Soil covering, mushroom management and harvesting

[0074] Covering: Cover the potted material with peat soil treated with 0.5% sodium hypochlorite solution and 2% quicklime to a thickness of 3-5 cm and cover with black film.

[0075] Fruiting Management: Three days before covering, the soil surface should be properly watered, following the principle of frequent, small amounts. After covering, turn off the ventilation system. When the material surface reaches a semi-black, semi-white state, gradually turn on the ventilation system and cool it down. Cooling to induce bud formation is done by slowly decreasing the temperature by 0.03-0.05°C / h, allowing the primordium to grow in a step-by-step manner to maximize yield. During the cooling period, turn on the ventilation system and slowly reduce the CO2 concentration in a gradient until it reaches 800-1600 ppm. Refer to the factory-produced Agaricus bisporus production process for fruiting management.

[0076] Harvesting: The harvesting standard is that the fruiting body is not fully mature, the cap diameter is 3-5cm, and the cap is not open. Gently rotate and pull to pick, and cut off part of the stem according to customer needs.

[0077] Example 2

[0078] 1. Shake flask strain preparation, inoculation and cultivation

[0079] a. Shake flask formula and preparation: formula

[0080] 200g potatoes, 20g glucose, 2g peptone, 2g soy flour, 1g potassium dihydrogen phosphate, 0.5g magnesium sulfate, 0.5g sodium chloride, 1L purified water (natural pH), 0.1mL defoamer;

[0081] Preparation: Boil potatoes in water, filter, take the filtrate, and adjust the volume. Use 1L Erlenmeyer flasks and put 600mL of solution in each bottle. Weigh the remaining raw materials in the Erlenmeyer flasks directly without boiling. Add the rotor, plug with silicone stoppers, and cover with kraft paper and a mask.

[0082] b. Sterilization and cooling

[0083] 121℃, sterilize for 30min; after sterilization, wait until the temperature drops to 65℃ and place it on a clean bench to cool naturally to 23-28℃.

[0084] c. Vaccination

[0085] Select a plate that is already covered with mycelium, take the middle part of the concentric mycelium on the plate, remove the aerial mycelium, take a sample the size of a pea, and inoculate 20 pieces into each bottle.

[0086] d. Cultivation

[0087] The shaker was set at 26°C and 150 rpm for 8 days. On the 6th day, the pellets were broken up using a stirrer. On the 8th day, when the mycelium culture reached the specified concentration, the grain strain was transferred.

[0088] 2. Fermenter Preparation, Inoculation, and Cultivation

[0089] a. Fermentation tank formula and preparation

[0090] Formula: Use a fermentation tank with a capacity of 800L, 21kg of white sugar, 1.4kg of corn flour, 1.4kg of peptone, 700g of potassium dihydrogen phosphate, 700g of magnesium sulfate, 350g of sodium chloride, 700L of purified water, 80mL of defoamer, and natural pH;

[0091] Preparation: Weigh the medicine bottle and dissolve it in warm water in a bucket. Transfer it to the fermentation tank, add purified water and defoamer, and clamp the air inlet.

[0092] b. Sterilization and cooling

[0093] 121℃, sterilize for 120min; after sterilization, wait until the temperature drops to 65℃ and transfer to the clean cooling workshop for air connection and forced cooling, and wait until the temperature cools to 23-28℃.

[0094] c. Shake flask strain selection

[0095] Use odorless, pollution-free bacteria with a concentration of 145-155 CFU / mL and a culture time of 8 days.

[0096] d. Vaccination

[0097] Use a flame gun and alcohol wipe to burn the inoculation port. Light a flame ring at the inoculation port to briefly burn and disinfect the shake flask surface. Slowly pour the shake flask culture into the shake flask. Reserve 50mL to test the shake flask for contamination.

[0098] e. Cultivation

[0099] The fermentation tank culture workshop temperature was set at 26°C, the ventilation pressure was set at 0.1-0.15 MPa, and the culture was carried out for 8 days.

[0100] 3. Preparation of grain culture, inoculation of liquid culture and cultivation

[0101] a. Grain strain formula and preparation

[0102] Formula: Wheat 98%, gypsum 2%, water content 50%, natural pH value;

[0103] Preparation: Soak wheat with 1% quicklime in water for 24 hours, pick up the wheat, wash it twice, put it into a large pot and simmer until there is no white core and it does not burst; drain the water from the wheat and mix it evenly with gypsum; put it into special culture bags, each bag contains 500g, and insert a punched glue stick in the middle.

[0104] b. Sterilization cooling

[0105] 121℃, sterilize for 120min; after sterilization, wait until the temperature drops to 65℃ and transfer to the clean cooling workshop for forced cooling until the temperature cools to 23-28℃.

[0106] c. Strain selection

[0107] Select liquid fermentation tank strains that are odorless, pollution-free, have a concentration of 150-160 CFU / mL, and a culture time of 8 days.

[0108] d. Vaccination

[0109] In the clean inoculation workshop, use a metering pump to evenly spray the liquid bacteria into the holes punched in the grains, with an inoculation amount of 1%.

[0110] e. Cultivation

[0111] The culture workshop was set at 26°C and 50% humidity, and cultured in the dark for 20-25 days.

[0112] 4. Preparation of clinker cultivation materials, inoculation of liquid bacteria, and cultivation

[0113] a. Recipe

[0114] Enoki mushroom residue 30%, wheat straw 20%, bran 20%, chicken manure 22%, gypsum 5%, lime 3%.

[0115] b. Preparation

[0116] The raw materials are weighed according to the ratio, and bran, gypsum and lime are first mixed evenly as the small ingredients; after the enoki mushroom residue, wheat straw and chicken manure are mixed evenly in a horizontal mixer, the small ingredients are added and mixed evenly, and the pH value is adjusted to 7.0-7.5 and the water content is adjusted to 65-70%.

[0117] c. Bottling and punching

[0118] Use high temperature and high pressure resistant plastic bottles, put 1kg of cultivation material in each bottle, make 5 holes in the cultivation material (specifications: 1.5cm diameter round hole, depth is the depth of the cultivation material), and put on a breathable cover.

[0119] d. Sterilization and cooling

[0120] 121℃, sterilize for 120min; after sterilization, wait until the temperature drops to 65℃ and transfer to the clean cooling workshop for forced cooling until the temperature cools to 23-28℃.

[0121] e. Strain selection

[0122] Use liquid culture to propagate grain cultures, and select high-quality cultures that are uncontaminated and unbroken.

[0123] f. Vaccination

[0124] In a clean inoculation workshop, use a quantitative instrument to inoculate the grain fungus into the holes in the bottled cultivation material, with an inoculation amount of 1%.

[0125] g. Cultivation

[0126] The culture room was set at 26°C and 60% humidity and cultured in the dark for 25 days.

[0127] h. Digging

[0128] The cultivated materials are dug out by air pump and transported to the mushroom bed through clean conveying pipes.

[0129] 5. Soil covering, mushroom management and harvesting:

[0130] Covering: The culture material dug out of the bottle is transported to the fruiting rack and covered with soil at the same time. Use peat soil treated with 0.5% sodium hypochlorite solution and 2% quicklime to cover the soil to a thickness of 3-5cm. After covering, cover with black film.

[0131] Fruiting Management: Three days before covering, the soil surface should be properly watered, adhering to the principle of frequent, small amounts. After covering, turn off the ventilation system. When the material surface reaches a semi-black, semi-white state, gradually turn on the ventilation system and cool it down. Cooling to induce bud formation is achieved by slowly decreasing the temperature by 0.03-0.05°C per hour, allowing the primordium to grow in a step-by-step manner to maximize yield. During the cooling period, turn on the ventilation system and slowly reduce the CO2 concentration in a gradient until it reaches 800-1600 ppm. Fruiting management should be based on factory-produced Agaricus bisporus production processes.

[0132] Harvesting: The harvesting standard is that the fruiting body is not fully mature, the cap diameter is 3-5cm, and the cap is not open. Gently rotate and pull to pick, and cut off part of the stem according to customer needs.

[0133] 6. Cultivation effect

[0134] In the above process, the process of using liquid spawn to cultivate Agaricus bisporus is shown in Figure 1 ; Plate culture, shake flask culture and fermentation tank submerged fermentation culture see Figure 2 ; The culture medium made from Pleurotus eryngii residue as the main raw material is shown in Figure 3 ; The cultivation of liquid bacteria in cultivation bottles or cultivation pots can be seen in Figure 4 ; Use liquid spawn cooked material to cultivate Agaricus bisporus mushroom fruiting frame diagram see Figure 5 .

[0135] The traditional cultivation process of Agaricus bisporus using grain fungus fermentation material uses grain fungus in granular form. The cultivation mode is fermentation material cultivation, mainly using single wheat straw and chicken manure as the main materials.

[0136] The cultivation effects of the traditional process, Example 1 and Example 2 were compared, and the comparison results are shown in Table 1:

[0137] Table 1 Comparison conclusion of different Agaricus bisporus cultivation techniques

[0138]

[0139] It can be seen from the above examples that when comparing the processes of Examples 1 and 2 with the traditional grain spawn fermentation material cultivation process, the overall effect of using liquid spawn mature material cultivation is better. Compared with the traditional grain spawn fermentation material cultivation process from the perspective of spawn preparation time, the present invention shortens the time by 44-51 days in Example 1 and 22-30 days in Example 2; compared with the traditional grain spawn fermentation material cultivation process from the perspective of cultivation material preparation and mycelial growth time, the present invention shortens the time by 18-26 days in Example 1 and 14-22 days in Example 2; compared with the traditional grain spawn fermentation material cultivation process from the perspective of average per square three-chao mushroom yield, the present invention increases the yield per square by 1.992kg in Example 1 and increases the yield per square by 0.928kg in Example 2. Compared with the cultivation material preparation, mycelial growth time and the average per square yield of mushrooms, the present invention is superior to the traditional grain spawn fermentation material cultivation process.

[0140] Compared to traditional processes, this invention can save significant investment costs. This invention involves cultivating Agaricus bisporus using liquid spawn and clinker, significantly simplifying the Agaricus bisporus production process. It eliminates the tedious primary and secondary fermentation processes, shortens spawn preparation time, reduces the probability of spawn contamination, and effectively reduces initial investment and production costs.

[0141] The embodiments described above are merely descriptions of preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by persons skilled in the art should fall within the scope of protection defined by the claims of the present invention.

Claims

1. A method for cultivating Agaricus bisporus using liquid spawn and cooked material, characterized in that: The following steps are involved: inoculating Agaricus bisporus into a shake flask culture medium, performing shake flask culture to obtain a shake flask strain, transferring the shake flask strain into a fermentation medium, performing fermentation culture to obtain a liquid strain; The liquid fungus is inoculated into the cultivation material and cultivated. After the cultivation is completed, the material is dug, soil is covered, the mushrooms are managed and harvested.

2. The method according to claim 1, wherein The raw materials of the shake flask culture medium include: 200 g of potatoes, 20 g of glucose, 2 g of peptone, 2 g of soy flour, 1 g of potassium dihydrogen phosphate, 0.5 g of magnesium sulfate, 0.5 g of sodium chloride, 1 L of purified water, and 0.1 mL of defoaming agent.

3. The method according to claim 1, wherein The shaking flask culture is carried out at a temperature of 23-28° C., a rotation speed of 140-160 rpm, and a culture time of 6-9 days.

4. The method according to claim 1, wherein The raw materials of the fermentation medium include: 21 kg of white sugar, 1.4 kg of corn flour, 1.4 kg of peptone, 700 g of potassium dihydrogen phosphate, 700 g of magnesium sulfate, 350 g of sodium chloride, 700 L of purified water and 80 mL of defoaming agent.

5. The method according to claim 1, wherein The fermentation culture temperature is 23-28° C., the ventilation pressure is 0.1-0.15 MPa, and the fermentation culture time is 6-9 days.

6. The method according to claim 1, wherein The raw materials of the cultivation material include: wood-rotting fungus residue, agricultural waste, gypsum, lime, ammonium sulfate and water.

7. The method according to claim 6, wherein The wood-rotting fungus residue is enoki mushroom residue or king oyster mushroom residue; the agricultural waste is livestock excrement or crop by-products.

8. The method according to claim 6, wherein The preparation of the planting material comprises the following steps: The raw materials are mixed and stirred, and packaged into 5 kg packages per pot, with 15-25 holes punched in each pot, sterilized, and cooled.

9. The method according to claim 1, wherein Calculated by mass fraction, the inoculation amount of the liquid bacteria is 1%.

10. The method according to claim 1, wherein The cultivation temperature is 23-28° C., the humidity is 50%-60%, and the cultivation time is 17-21 days.

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