Application of ansamitocin P-3 in promoting corn growth and improving insect resistance

Abstract

The invention discloses application of ansamitocin P-3 in promotion of corn growth and improvement of insect resistance. The ansamitocin P-3 ensures the plant health by improving the growth index and the plant-feeding insect feeding resistance of the corn; the plant growth promoter with ansamitocin P-3 as a component is mixed and applied in soil where plants grow in a water solution mode, the plant height and biomass of the overground part of corn seedlings are remarkably improved through mixing and application, and root development is not affected; the insect resistance of the corn is also remarkably improved, and the gnawing area of the spodoptera frugiperda and the growth rate of larvae are remarkably reduced. According to the method, the specific growth promoter is mixed and applied to the corn, the corn seedling strengthening and insect resisting effects under the normal condition can be achieved, the method is economical, simple, convenient, green and efficient, and grain production is guaranteed.

Description

Technical Field

[0001] The present invention relates to the field of agricultural plant growth promotion, and in particular to an application of ansamitocin P-3 in promoting corn growth and insect resistance. Background Art

[0002] Corn is an important global crop for food, feed, and industrial raw materials. Its production is crucial to ensuring food security and supporting animal husbandry and industrial development. However, the growth and yield of corn are often threatened by a variety of factors, and insect pests are one of the key factors that lead to seedling damage, yield reduction, and even crop failure. Take the fall armyworm (Spodoptera frugiperda) as an example. It has been included in the "List of Major Pests and Diseases". Its larvae feed on corn leaves, stems, and ears, which can lead to crop yield reduction or even crop failure. Although the use of traditional chemical pesticides can control pests in the short term, long-term use can easily induce pest resistance and bring about environmental pollution problems.

[0003] The use of plant growth promoters can effectively enhance plant growth and resistance, transcending environmental constraints for direct agricultural application. They not only reduce reliance on chemical pesticides but also promote stable yields and increased efficiency in the corn industry amidst frequent pest infestations and pressures on sustainable arable land use, possessing significant ecological and economic value. Therefore, developing a plant growth promoter with both growth-promoting and stress-resistant properties would provide an innovative solution for green corn production. Summary of the Invention

[0004] To address the aforementioned issues, the present invention provides a novel application of ansamitocin P-3 as a plant growth promoter to promote corn seedling growth and enhance plant insect resistance. By applying ansamitocin P-3, a specific growth promoter, to corn, the present invention achieves strong corn seedlings and insect resistance under normal conditions. This method is economical, simple, environmentally friendly, and highly effective, ensuring food production.

[0005] The technical solution adopted in the present invention is:

[0006] 1. An application of ansamitocin P-3, including application in promoting corn growth and resisting insects.

[0007] The molecular formula of ansamitocin P-3 is C 32 H 43 ClN2O9, which appears as a white solid powder, has multiple pharmacological activities such as anti-tumor, anti-tuberculosis, and anti-bacterial.

[0008] The ansamitocin P-3 improves the growth index and insect resistance of corn.

[0009] The ansamitocin P-3 is used to promote the growth of corn seedlings.

[0010] The insect-resistant objects described in the present invention are insects.

[0011] The insect to be treated is the second instar of the lepidopteran herbivorous insect Spodotera frugiperda J2s.

[0012] 2. A corn growth promoter, comprising ansamitocin P-3.

[0013] 3. A method for promoting corn growth using ansamitocin P-3:

[0014] Applying ansamitocin P-3 solution to the soil where corn seedlings are planted can significantly improve the growth indicators and insect resistance of corn, thereby achieving the purpose of promoting seedling growth.

[0015] The concentration of the ansamitocin P-3 solution used for mixing and applying is 0.125-5.0 μg / kg fresh soil.

[0016] The dosage of the ansamitocin P-3 solution used for mixing and applying is about 0.025 to 1 μg per plant.

[0017] In a specific implementation, the concentrations of the ansamitocin P-3 solution used for mixing and application are 0.125 μg / kg, 0.5 μg / kg and 5.0 μg / kg fresh soil, and the corresponding dosage per plant is approximately 0.025, 0.1 and 1 μg / plant.

[0018] The growth indicators for promoting corn growth in specific implementation include any one of:

[0019] 1) Increase the height of the above-ground plant;

[0020] 2) Increase the fresh weight and dry weight of the aboveground plant;

[0021] 3) Increase the fresh weight and dry weight of the underground part of the plant.

[0022] The specific indicators for promoting corn insect resistance include any one of the following:

[0023] 1) Reduce the growth rate of fall armyworm larvae;

[0024] 2) Reduce the area of ​​corn leaves eaten by fall armyworms.

[0025] The present invention uses corn and ansamitocin P-3 as research objects. Ansamitocin P-3 is evenly mixed into the soil at different concentration gradients, and then corn sprouts are planted. The growth status of the corn plants is observed 20 days later. The control group is treated with clean water. It is found that compared with the control group, the corn planted in the soil treated with ansamitocin P-3 has a higher plant height and significantly increased fresh weight and dry weight of the aboveground part.

[0026] To determine whether ansamitocin P-3 improves insect resistance in corn, the present invention tested 20-day-old corn inoculated with insects. It was found that, compared with the control group, three days after inoculation with second-instar fall armyworm, the weight gain rate of corn planted in soil treated with ansamitocin P-3 decreased, as did the amount of corn leaf feeding, indicating that even low-concentration treatments can achieve insect resistance in corn.

[0027] The present invention provides the following technical solutions:

[0028] The plant growth promoter provided by the present invention uses ansamitocin P-3 as an active ingredient. After being dissolved in water, it is evenly mixed into the soil. The plant height, fresh weight and dry weight of the aboveground part, and fresh weight of the underground part of corn seedlings are significantly increased; and the growth rate of the larvae of the herbivorous insect Spodoptera frugiperda and the amount of food they eat on the corn seedlings are reduced.

[0029] The scheme of the present invention is simple, green and economical, and can be applied to agricultural production and related research fields to ensure corn crop production and insect resistance to fall armyworm. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a graph showing the effect of applying 0.125 μg / kg, 0.5 μg / kg and 5.0 μg / kg of ansamitocin P-3 on the aboveground plant height of corn after 20 days.

[0031] Figure 2 This is a graph showing the effects of 0.125 μg / kg, 0.5 μg / kg and 5.0 μg / kg of ansamitocin P-3 mixed application on the fresh weight of above-ground and underground parts of corn at 20 days.

[0032] Figure 3 This is a graph showing the effect of 0.125 μg / kg, 0.5 μg / kg and 5.0 μg / kg of ansamitocin P-3 mixed application on the dry weight of the above-ground and underground parts of corn after 20 days.

[0033] Figure 4 This is a graph showing the effects of applying 0.125 μg / kg, 0.5 μg / kg and 5.0 μg / kg of ansamitocin P-3 on the growth rate of corn larvae 20 days after inoculation with Spodoptera frugiperda.

[0034] Figure 5 This is a graph showing the effects of applying 0.125μg / kg, 0.5μg / kg and 5.0μg / kg of ansamitocin P-3 on the area eaten by the fall armyworm. DETAILED DESCRIPTION

[0035] The following examples are provided to illustrate the present invention but are not intended to limit the scope of the invention. Any modifications or substitutions to the methods, steps, or conditions of the present invention without departing from the spirit and substance of the present invention are intended to be within the scope of the present invention. The methods described are conventional methods unless otherwise specified, and the materials described are commercially available unless otherwise specified.

[0036] The embodiments of the present invention are as follows:

[0037] Example 1

[0038] This embodiment relates to the application of ansamitocin P-3 mixed with soil to promote corn growth.

[0039] 1. Agents, corn materials and methods for accelerating seedlings

[0040] Pure ansamitocin P-3 (CAS: 66584-72-3) was purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (98%) and dissolved in Milli-Q to prepare a 1 mg / mL stock solution for subsequent use. Test soil was obtained from a farmland in Hangzhou, Zhejiang. The corn variety used was B73 (Zea mays L). Seeds were soaked overnight and then seeded in the dark on plates covered with moistened filter paper for two days.

[0041] 2. Ansamitocin P-3 mixed application experimental treatment

[0042] Different volumes of ansamitocin P-3 stock solution (1 mg / mL) were diluted 100-fold with water to a 10 μg / mL solution. 62.5 μL, 250 μL, and 2500 μL were taken, respectively, and diluted to 500 mL. The solution was then applied to 4.5 kg of soil and stirred evenly, resulting in 5 kg of test soil with final concentrations of 0.125 μg / kg, 0.5 μg / kg, and 5.0 μg / kg of ansamitocin P-3. A control group consisted of the same volume of soil applied with milli-Q solution without ansamitocin P-3. The soil was then evenly distributed into pots (200 g of soil / pot), and one germinated corn B73 seed was sown in each pot, equivalent to treatments of 0.025, 0.1, and 1 μg of ansamitocin P-3 per corn seedling. The potted plants were placed in a greenhouse at a temperature of 26.5°C, a humidity of 55%, and a carbon dioxide concentration of 456 ppm for 20 days, during which time they were watered appropriately every day to ensure the normal growth of the corn.

[0043] 3. Index determination

[0044] Corn biomass determination: After 20 days of planting corn seedlings in soils with different ansamitocin P-3 concentrations, the plant height was measured with a ruler. The aboveground and underground tissues of corn were collected and the fresh weight was measured; after drying, the dry weight was measured ( Figure 1-Figure 3).

[0045] 4. Experimental results

[0046] like Figure 1-Figure 3 As shown in the results, the aboveground plant height and biomass of corn planted in the soil after application of ansamitocin P-3 were significantly higher than those in the control group without application, and the underground biomass showed an increasing trend at high application concentrations.

[0047] like Figure 1 As shown, compared with the control, the plant height of the aboveground part of corn increased significantly at the concentrations of 0.5 and 5 μg / kg, with the increase rate being 6.9%.

[0048] like Figure 2 As shown in the results, compared with the control, the fresh weight of the aboveground part of corn increased significantly at 0.5 and 5 μg / kg concentrations, with weight increases of 31.9% and 37.7%, respectively. The fresh weight of the underground part increased significantly at 5.0 μg / kg concentration (the highest concentration), with a weight increase of 26.3% compared with the control.

[0049] like Figure 3 As shown in the results, compared with the control, the dry weight of the aboveground part of corn increased significantly at 0.5 and 5 μg / kg concentrations, with weight increases of 32.8% and 23.8% respectively. The dry weight of the underground part showed a weight increase trend at 5.0 μg / kg concentration (i.e., the highest concentration).

[0050] Based on the above results, it was concluded that applying ansamitocin P-3 to the soil for planting corn can promote the growth of corn seedlings, among which the application of 0.5μg / kg concentration can maximize the weight gain of the aboveground part; the application of 5.0μg / kg concentration can maximize the weight gain of the underground part.

[0051] In other words, the application of ansamitocin P-3 can promote the growth of corn seedlings.

[0052] Example 2

[0053] This embodiment relates to the use of ansamitocin P-3 as a mixed application to improve the resistance of corn to herbivorous insects.

[0054] 1. Ansamitocin P-3 application test method and index determination

[0055] Pure ansamitocin P-3, test corn seeds and seeding, planting, test soil, and greenhouse conditions were similar to those in Example 1. Planting was carried out under greenhouse conditions for 20 days with daily watering. Second-instar fall armyworm larvae (Spodotera frugiperda J2s) were purchased from Baiyun Industrial Co., Ltd., Jiyuan, Henan Province.

[0056] Fall Armyworm Inoculation Treatment: When corn is 17 days old, pre-starved, second-instar Fall Armyworm larvae (initial weight: 1.7 ± 0.3 mg) are selected and placed on the fourth fully expanded leaf for three days to simulate an infestation. A 2 cm diameter mesh cage is secured upside down on the leaf using a clamp to restrict the larvae's range of movement. Feeding and activity levels are checked periodically, and the cage is adjusted to maintain adequate leaf supply and movement space.

[0057] Every 36 hours over three days, the area of ​​corn leaves eaten by fall armyworms was collected twice, spread flat on paper, and scanned. Digimizer (5.6.0) software was used to calculate the area of ​​corn eaten by fall armyworms during the two days. The total was calculated as the total amount of corn eaten by fall armyworms over the three-day period. After three days, the final weight of the fall armyworms was recorded, and the growth rate was calculated using the following formula:

[0058] Worm growth rate (weight gain / day) = [(final worm weight per replicate - initial worm weight) / initial worm weight] / number of days

[0059] 2. Experimental results

[0060] like Figure 4-Figure 5 As shown in the figure, the insect resistance of 20-day-old corn planted in the soil after application of ansamitocin P-3 was significantly improved compared with the control.

[0061] like Figure 4 As shown, compared with the control, soil application of 0.5 μg / kg and 5.0 μg / kg of ansamitocin P-3 can significantly reduce the leaf gnawing area of ​​the fall armyworm, and the concentration of 0.125 μg / kg has a decreasing trend on the leaf gnawing damage of the fall armyworm.

[0062] like Figure 5 As shown, compared with the control, 0.125μg / kg, 0.5μg / kg and 5.0μg / kg of ansamitocin P-3 mixed with soil planting can significantly reduce the weight gain rate of Spodoptera frugiperda.

[0063] In summary, the application of low concentration of ansamitocin P-3 can achieve the result of improving corn resistance.

[0064] This study demonstrates that a plant growth promoter containing ansamitocin P-3, when applied as a low-concentration aqueous solution to the soil where plants are growing, significantly increased the above-ground height and biomass of corn seedlings compared to a control group (applied with plain water) without affecting root development. Ansamitocin P-3 also significantly improved corn's insect resistance, with a significant decrease in the feeding area and larval growth rate of the fall armyworm (Spodoptera frugiperda).

[0065] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. An application of ansamitocin P-3, characterized in that: Including applications in promoting corn growth and insect resistance.

2. The use of ansamitocin P-3 according to claim 1, characterized in that: The ansamitocin P-3 improves the growth index and insect resistance of corn.

3. The use of ansamitocin P-3 according to claim 1, characterized in that: The ansamitocin P-3 is used to promote the growth of corn seedlings.

4. The use of ansamitocin P-3 according to claim 1, characterized in that: The anti-insect target is an insect.

5. The use of ansamitocin P-3 according to claim 4, characterized in that: The insect is the second instar of the herbivorous insect Spodotera frugiperda J2s of the Lepidoptera order.

6. A corn growth promoter, characterized in that Including ansamitocin P-3.

7. A method for promoting corn growth using ansamitocin P-3, characterized in that: Applying ansamitocin P-3 solution to the soil where corn seedlings are planted can significantly improve the growth indicators and insect resistance of corn, thereby achieving the purpose of promoting seedling growth.

8. The method for promoting corn growth using ansamitocin P-3 according to claim 7, characterized in that: The concentration of the ansamitocin P-3 solution is 0.125-5.0 μg / kg fresh soil.

9. The method for promoting corn growth using ansamitocin P-3 according to claim 7, characterized in that: The usage amount of the ansamitocin P-3 solution corresponding to the plant is about 0.025 to 1 μg per plant.

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