Eurotium cristatum-based eurotium cristatum growing process of Ninghong golden camellia

Abstract

The invention relates to the technical field of tea leaf processing, in particular to a eurotium cristatum-based Ninghong golden camellia eurotium cristatum growing process, by arranging temperature control equipment and humidifying equipment, the environment temperature and humidity of the eurotium cristatum growing process can be accurately controlled, and the eurotium cristatum growing process can be realized through operation of a motor. According to the device, eurotium cristatum strains can be communicated with all the fermentation chambers, uniform injection operation of the same and quantitative strains in all the fermentation chambers can be achieved through driving of the electric push rod, fermentation parameters can be accurately controlled, effective control over the floating process is achieved, the quality of Ninghong golden camellia is remarkably improved, and the production efficiency is improved. The taste, aroma and soup color of the tea leaves are improved.

Description

Technical Field

[0001] The invention relates to a flowering process, in particular to a flowering process of Ninghong Camellia chrysantha based on Eurotium cristatum. Background Art

[0002] Tea flowering refers to the process in which specific beneficial microbial colonies grow inside or on the surface of tea leaves under specific environmental conditions such as temperature and humidity. Through the flowering of tea, the taste and flavor of tea can be improved, the quality stability of tea can be enhanced, and the health benefits of tea can be increased.

[0003] Currently, finished Ninghong Jinhua tea products on the market generally suffer from a bitter taste, poor flavor, and weak aroma. Traditional techniques for producing Ninghong Jinhua tea, a crucial step in tea processing, suffer from a low success rate. This makes it difficult to achieve the desired flowering effect for large quantities of tea, resulting in a waste of resources. Furthermore, inconsistent quality is a prominent issue, with significant variations in aroma, flavor, and tea color between batches. This makes it difficult to meet consumers' demand for consistent quality, severely hindering the further development of the Ninghong Jinhua tea industry.

[0004] Based on the above reasons, the present invention proposes a Ninghong Camellia chrysantha flowering process based on Eurotium cristatum, which can stably improve the flowering success rate, accurately control the quality of tea, and create Ninghong Camellia chrysantha products with unique flavor and stable quality, thereby promoting the healthy and sustainable development of the Ninghong Camellia chrysantha industry. Summary of the Invention

[0005] In view of the shortcomings of the existing technology, the present invention provides a flowering process of Ninghong Camellia chrysantha based on Eurotium cristatum.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0007] The Ninghong Camellia chrysantha flowering process based on Eurotium cristatum comprises the following steps:

[0008] S1: Raw material selection and processing: Select fresh Ninghong Golden Camellia leaves from summer and autumn, wash and wither them, and control the moisture content of the fresh leaves to 60%-70%;

[0009] S2: Preparation of Eurotium cristatum strains: Screen the Eurotium cristatum strains and expand the culture to obtain sufficient number of strains with good activity;

[0010] S3: Inoculation and fermentation parameter adjustment: The cultured Eurotium cristatum strain was evenly inoculated onto the treated Ninghongjinhua tea leaves, and the inoculated tea leaves were placed in a fermentation room, with the temperature controlled at 25°C-30°C and the humidity at 65%-75%. The fermentation time was 10-15 days, with regular ventilation during the fermentation period.

[0011] S4: Quality testing and control: During the fermentation process, the tea leaves are tested for quality every two days, including aroma, taste, soup color and other indicators, and fermentation parameters are fine-tuned based on the test results;

[0012] S5: Drying and packaging: After fermentation, the tea leaves are dried at low temperature and the dried Ninghong Jinhua tea is packaged.

[0013] As a preferred technical solution of the present invention, the cultivation of the Eurotium cristatum strain in step S2 includes the following steps:

[0014] S21: Under a sterile operating environment, the tea sample was cut into small pieces and placed in a culture dish containing a specific culture medium. The culture medium composition includes 10g / L glucose, 5g / L peptone, 3g / L yeast extract, 2g / L potassium dihydrogen phosphate, 0.5g / L magnesium sulfate, and 20g / L agar. The pH value was adjusted to 6.5-7.0.

[0015] S22: placing the culture dish in a constant temperature incubator at a temperature of 28°C-30°C for 3-5 days, and after the colony characteristics of Eurotium cristatum appear, performing isolation and purification by a plate streak method, and repeating the isolation and purification operation at least 3 times to ensure that a single pure species of Eurotium cristatum is obtained;

[0016] S23: The purified strains are inoculated into liquid culture medium for expansion culture. The composition of the liquid culture medium is similar to the above-mentioned solid culture medium, except that agar is not added. The liquid culture medium is cultured in a shaker at a speed of 150-200 r / min and a temperature of 28°C-30°C for 48-72 hours to obtain sufficient number of strains with good activity.

[0017] As a preferred technical solution of the present invention, the colony of Eurotium cristatum in step S22 is characterized by being golden yellow and having fuzzy hyphae on the surface.

[0018] As a preferred technical solution of the present invention, the inoculation and fermentation parameter adjustment equipment in step S3 includes a round shell, a number of door panels are evenly distributed and installed on the side walls of the round shell, the inner cavity of the round shell is fixedly connected to partitions on both sides of the door panels, the side walls of the partitions are fixedly connected to temperature control equipment and humidification equipment, several of the partitions are fixedly connected to the same inner shell and top plate, the side walls of the inner shell located between the partitions are fixedly connected to temperature sensors, humidity sensors and visual equipment, movable components are arranged in the inner shell, a number of inoculation control components are evenly distributed and fixedly connected on the top surface of the top plate, and a feed shell is fixedly connected to the top surface of the round shell.

[0019] As a preferred technical solution of the present invention, the movable component includes a fixed plate, which is fixedly installed in the inner cavity of the inner shell and is fixedly connected to a motor on the top surface, a rotating shaft is fixedly connected to the rotor of the motor, and a circular plate is fixedly connected to the top of the rotating shaft, and a number of side grooves are provided on the top plate, each of which is movably connected to a movable plate and a nozzle is fixedly connected to the bottom surface, a through groove is provided on the movable plate and is hingedly connected to a connecting plate, and the connecting plate extends out of the side groove and is hingedly connected to the circular plate.

[0020] As a preferred technical solution of the present invention, the inoculation control component includes a fixed shell, which is fixedly connected to the top surface of the top plate, and a circular tube that passes through the feed shell is fixedly connected to the side wall of the fixed shell. A push plate is provided in the inner cavity of the fixed shell, and a movable rod is fixedly connected to the top surface of the push plate. The movable rod passes through the fixed shell and the top surface of the circular shell and is fixedly connected to the same annular plate, and an electric push rod is fixedly connected to the annular plate, and the electric push rod is fixedly connected to the circular shell.

[0021] As a preferred technical solution of the present invention, a transparent plate and a handle are fixedly connected to the door panels.

[0022] As a preferred technical solution of the present invention, in step S4, hot air drying is adopted, and the tea leaves are evenly spread on the tray of the drying equipment with a thickness of 2-3 cm. The hot air temperature is set to 50°C-60°C, the wind speed is 0.5-1 m / s, and the drying time is adjusted according to the moisture content of the tea leaves and the batch size, and is 4-6 hours, until the moisture content of the tea leaves is less than 8%.

[0023] The embodiment of the present invention provides a Ninghong Camellia chrysantha flowering process based on Eurotium cristatum, which has the following characteristics:

[0024] Beneficial effects:

[0025] 1. By carefully selecting local Eurotium cristatum strains and optimizing the culture and inoculation conditions of the strains, the present invention increases the flowering success rate by 30%-50% compared with the traditional process, effectively reducing the waste of resources caused by flowering failure;

[0026] 2. The present invention is equipped with a temperature control device and a humidifying device, which can achieve precise control of the ambient temperature and humidity of the flowering process. The operation of the motor can realize the connection between the Aspergillus niger strains and each fermentation chamber, and the drive of the electric push rod can realize the uniform filling operation of the same and quantitative strains into each fermentation chamber, accurately control the fermentation parameters, realize effective control of the flowering process, significantly improve the quality of Ninghong Jinhua tea, and improve the taste, aroma and soup color of the tea. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0028] Figure 1 This is a flow chart of the flower blooming process of the present invention;

[0029] Figure 2 It is a flow chart of the method for culturing Eurotium cristatum strains of the present invention;

[0030] Figure 3 It is a schematic diagram of the overall three-dimensional structure of the inoculation and fermentation parameter adjustment device of the present invention;

[0031] Figure 4 It is a schematic diagram of the internal cross-sectional three-dimensional structure of the inoculation and fermentation parameter adjustment device of the present invention;

[0032] Figure 5 This is a schematic diagram of the front three-dimensional structure of the inner cavity of the circular shell of the present invention;

[0033] Figure 6 It is a schematic diagram of the side three-dimensional structure of the inner cavity of the circular shell of the present invention;

[0034] Figure 7 It is a schematic diagram of the three-dimensional structure of the movable component of the present invention.

[0035] In the figure: 1. round shell; 2. door panel; 3. partition; 4. temperature control device; 5. humidification device; 6. inner shell; 7. top plate; 8. temperature sensor; 9. humidity sensor; 10. visual device; 11. movable component; 12. inoculation control component; 13. feed shell; 14. fixed plate; 15. motor; 16. rotating shaft; 17. round plate; 18. side groove; 19. movable plate; 20. nozzle; 21. through groove; 22. connecting plate; 23. fixed shell; 24. round tube; 25. push plate; 26. movable rod; 27. ring plate; 28. electric push rod; 29. ​​transparent plate; 30. handle. DETAILED DESCRIPTION

[0036] The preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0037] Example 1: Figure 1-7 As shown, the Ninghong Camellia chrysantha flowering process based on Eurotium cristatum comprises the following steps:

[0038] S1: Raw material selection and processing: Select fresh Ninghong Golden Camellia leaves from summer and autumn, wash and wither them, and control the moisture content of the fresh leaves to 60%-70%;

[0039] S2: Preparation of Eurotium cristatum strains: Screen the Eurotium cristatum strains and expand the culture to obtain sufficient number of strains with good activity;

[0040] Specifically, the cultivation of the Eurotium cristatum strain includes the following steps:

[0041] S21: Under a sterile operating environment, the tea sample was cut into small pieces and placed in a culture dish containing a specific culture medium. The culture medium composition includes 10g / L glucose, 5g / L peptone, 3g / L yeast extract, 2g / L potassium dihydrogen phosphate, 0.5g / L magnesium sulfate, and 20g / L agar. The pH value was adjusted to 6.

[0042] S22: placing the culture dish in a constant temperature incubator and culturing at a temperature of 28° C. for 4 days, and after the colony characteristics of the cristatum appear, the colony characteristics of the cristatum are golden yellow with fuzzy hyphae on the surface, and performing isolation and purification by the plate streak method. Repeat the isolation and purification operation at least 3 times to ensure that a single pure species of the cristatum is obtained;

[0043] S23: The purified strains are inoculated into liquid culture medium for expansion culture. The composition of the liquid culture medium is similar to the above-mentioned solid culture medium, except that agar is not added. The liquid culture medium is cultured in a shaker at a speed of 180 r / min and a temperature of 28°C for 48 hours to obtain sufficient number of strains with good activity.

[0044] S3: Inoculation and fermentation parameter adjustment: The cultured Eurotium cristatum strain was evenly inoculated onto the treated Ninghongjinhua tea leaves, and the inoculated tea leaves were placed in a fermentation room, with the temperature controlled at 25°C and the humidity at 70%. The fermentation time was 12 days, with regular ventilation during the fermentation period.

[0045] Specifically, the inoculation and fermentation parameter adjustment device includes a round shell 1, a plurality of door panels 2 are evenly distributed and installed on the side wall of the round shell 1, and a transparent plate 29 and a handle 30 are fixedly connected to the door panels 2;

[0046] In the technical solution of this embodiment, during the flowering process of Ninghong Golden Camellia, the door panel 2 can be closed by the handle 30 to seal the inner cavity of the round shell 1, reducing the influence of the external environment on the flowering process of Ninghong Golden Camellia, and the transparent plate 29 can facilitate the operator to directly observe the situation inside the fermentation chamber, which is convenient to use;

[0047] The inner cavity of the circular shell 1 is fixedly connected to partitions 3 on both sides of the door panel 2. The side walls of the partitions 3 are fixedly connected to temperature control equipment 4 and humidification equipment 5. Several partitions 3 are fixedly connected to the same inner shell 6 and top plate 7. The side walls of the inner shell 6 located between the partitions 5 are fixedly connected to temperature sensors 8, humidity sensors 9 and visual equipment 10.

[0048] In the technical solution of this embodiment, during the flowering operation of Ninghong Golden Camellia, the fermentation chambers between the partitions 3 can be heated by the control device 4, and the temperature inside each fermentation chamber can be accurately controlled by the temperature sensor 8. The inside of the fermentation chamber can also be humidified by the humidifying device 5, and the humidity can be monitored by the humidity sensor 9. As a result, the flowering process of Ninghong Golden Camellia based on Eurotium cristatum has accurately controllable temperature and humidity, significantly improving the quality of Ninghong Golden Camellia, improving the taste, aroma and soup color of the tea, and the visual device 10 can be used to collect image data of the process, which is convenient to use.

[0049] A movable assembly 11 is provided in the inner shell 6. The movable assembly 11 includes a fixed plate 14. The fixed plate 14 is fixedly mounted on the inner cavity of the inner shell 6 and fixedly connected to a motor 15 on the top surface. A rotating shaft 16 is fixedly connected to the rotor of the motor 15. A circular plate 17 is fixedly connected to the top of the rotating shaft 16. A plurality of side grooves 18 are provided on the top plate 7. A movable plate 19 is movably connected in each of the side grooves 18 and a nozzle 20 is fixedly connected to the bottom surface. A through groove 21 is provided on the movable plate 19 and is hingedly connected to a connecting plate 22. The connecting plate 22 extends out of the side grooves 18 and is hingedly connected to the circular plate 17.

[0050] In the technical solution of this embodiment, the motor 15 is started, and the motor 15 drives the circular plate 17 on the rotating shaft 16 to rotate, and the circular plate 17 drives the connecting plate 22 to move, and the connecting plate 22 pulls the movable plate 19 to move in the side groove 18, so that the through groove 21 on the movable plate 19 and the fixed shell 23 are concentrically connected, and the opening and closing control of the filling of the crown-like fungus can be realized;

[0051] Several inoculation control components 12 are evenly distributed and fixedly connected on the top surface of the top plate 7, and a feed shell 13 is fixedly connected to the top surface of the round shell 1. The inoculation control component 12 includes a fixed shell 23, which is fixedly connected to the top surface of the top plate 7. A circular tube 24 that penetrates the feed shell 13 is fixedly connected to the side wall of the fixed shell 23. A push plate 25 is provided in the inner cavity of the fixed shell 23, and a movable rod 26 is fixedly connected to the top surface of the push plate 25. The movable rod 26 passes through the fixed shell 23 and the top surface of the round shell 1 and is fixedly connected to the same annular plate 27. An electric push rod 28 is fixedly connected to the annular plate 27, and the electric push rod 28 is fixedly connected to the round shell 1;

[0052] In the technical solution of this embodiment, by adding the cultured Eurotium guanyceae strain into the feed shell 13, the Eurotium guanyceae strain enters the inner cavity of the fixed shell 23 through the circular tube 24. When the Eurotium guanyceae strain is inoculated, the electric push rod 28 is started, and the electric push rod 28 drives the annular plate 27 to move. The annular plate 27 drives the push plate 25 to move through the movable rod 26. The push plate 25 is used to squeeze the Eurotium guanyceae strain in the inner cavity of the fixed shell 23, so that the Eurotium guanyceae strain can be uniformly inoculated into the tea leaves placed between the partitions 3 through the nozzle 20, thereby achieving precise control of the inoculation of the Eurotium guanyceae strain and improving the effect of flowering.

[0053] S4: Quality testing and control: During the fermentation process, the tea leaves are tested for quality every two days, including aroma, taste, soup color and other indicators, and fermentation parameters are fine-tuned based on the test results;

[0054] S5: Drying and packaging: After fermentation, the tea leaves are dried at low temperature and dried with hot air. The tea leaves are evenly spread on the tray of the drying equipment with a thickness of 2 cm. The hot air temperature is set to 55°C and the wind speed is 0.8 m / s. The drying time is 5 hours until the moisture content of the tea leaves is less than 8%. The dried Ninghong Jinhua tea is then packaged.

[0055] After testing, the success rate of flowering reached 80%, and the tea quality was excellent, with mellow taste and rich aroma.

[0056] Example 2: Repeat the steps of Example 1, except that the temperature in the fermentation chamber after inoculation is controlled at 28°C. The remaining operations are inconvenient;

[0057] After testing, the success rate of flowering reached 88%, and the tea quality was excellent, with mellow taste and rich aroma.

[0058] Example 3: Repeat the steps of Example 1, except that the temperature in the fermentation chamber after inoculation is controlled at 30°C. The remaining operations are inconvenient;

[0059] After testing, the success rate of flowering reached 83%, and the tea quality was excellent, with mellow taste and rich aroma.

[0060] In summary, after adopting the process of the present invention, the flowering success rate is generally improved to more than 80%. In particular, when the temperature in the fermentation chamber after inoculation is controlled at 28°C, the humidity is at 70%, and the fermentation time is 10 days, the flowering success rate reaches 88%, effectively reducing the waste of resources caused by flowering failure.

[0061] Finally, it should be noted that in the description of the present invention, it should be noted that the terms "vertical", "up", "down", "horizontal", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they cannot be understood as limiting the present invention.

[0062] In the description of the present invention, it should also be noted that, unless otherwise expressly specified or limited, the terms "disposed," "installed," "connected," and "connected" should be understood in a broad sense. For example, they may refer to fixed connections, detachable connections, or integral connections; they may refer to mechanical connections or electrical connections; they may refer to direct connections or indirect connections through an intermediate medium; and they may refer to internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0063] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein with equivalents. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. The Ninghong Camellia chrysantha flowering process based on Eurotium cristatum is characterized in that: The following steps are involved: S1: Raw material selection and processing: Select fresh Ninghong Golden Camellia leaves from summer and autumn, wash and wither them, and control the moisture content of the fresh leaves to 60%-70%; S2: Preparation of Eurotium cristatum strains: Screen the Eurotium cristatum strains and expand the culture to obtain sufficient number of strains with good activity; S3: Inoculation and fermentation parameter adjustment: The cultured Eurotium cristatum strain was evenly inoculated onto the treated Ninghongjinhua tea leaves, and the inoculated tea leaves were placed in a fermentation room, with the temperature controlled at 25°C-30°C and the humidity at 65%-75%. The fermentation time was 10-15 days, with regular ventilation during the fermentation period. S4: Quality testing and control: During the fermentation process, the tea leaves are tested for quality every two days, including aroma, taste, soup color and other indicators, and fermentation parameters are fine-tuned based on the test results; S5: Drying and packaging: After fermentation, the tea leaves are dried at low temperature and the dried Ninghong Jinhua tea is packaged.

2. The Ninghong Camellia chrysantha flowering process based on Eurotium cristatum according to claim 1, characterized in that: The cultivation of the Eurotium cristatum strain in step S2 comprises the following steps: S21: Under a sterile operating environment, the tea sample was cut into small pieces and placed in a culture dish containing a specific culture medium. The culture medium composition includes 10g / L glucose, 5g / L peptone, 3g / L yeast extract, 2g / L potassium dihydrogen phosphate, 0.5g / L magnesium sulfate, and 20g / L agar. The pH value was adjusted to 6.5-7.

0. S22: placing the culture dish in a constant temperature incubator at a temperature of 28°C-30°C for 3-5 days, and after the colony characteristics of Eurotium cristatum appear, performing isolation and purification by a plate streak method, and repeating the isolation and purification operation at least 3 times to ensure that a single pure species of Eurotium cristatum is obtained; S23: The purified strains are inoculated into liquid culture medium for expansion culture. The composition of the liquid culture medium is similar to the above-mentioned solid culture medium, except that agar is not added. The liquid culture medium is cultured in a shaker at a speed of 150-200 r / min and a temperature of 28°C-30°C for 48-72 hours to obtain sufficient number of strains with good activity.

3. The Ninghong Camellia chrysantha flowering process based on Eurotium cristatum according to claim 2, characterized in that, The colony of Eurotium cristatum in step S22 is characterized by being golden yellow and having fuzzy hyphae on the surface.

4. The Ninghong Camellia chrysantha flowering process based on Eurotium cristatum according to claim 1, characterized in that: The inoculation and fermentation parameter adjustment equipment in step S3 comprises a circular shell (1), a plurality of door panels (2) are evenly distributed and installed on the side walls of the circular shell (1), a partition (3) is fixedly connected to the inner cavity of the circular shell (1) on both sides of the door panels (2), a temperature control device (4) and a humidifying device (5) are fixedly connected to the side walls of the partitions (3), a plurality of the partitions (3) are fixedly connected to the same inner shell (6) and top plate (7), a temperature sensor (8), a humidity sensor (9) and a visual device (10) are fixedly connected to the side walls of the inner shell (6) located between the partitions (5), a movable component (11) is arranged in the inner shell (6), a plurality of inoculation control components (12) are evenly distributed and fixedly connected on the top surface of the top plate (7), and a feed shell (13) is fixedly connected to the top surface of the circular shell (1).

5. The Ninghong Camellia chrysantha flowering process based on Eurotium cristatum according to claim 4, characterized in that: The movable assembly (11) includes a fixed plate (14), which is fixedly mounted on the inner cavity of the inner shell (6) and fixedly connected to a motor (15) on the top surface. A rotating shaft (16) is fixedly connected to the rotor of the motor (15), and a circular plate (17) is fixedly connected to the top end of the rotating shaft (16). A plurality of side grooves (18) are provided on the top plate (7), and movable plates (19) are movably connected in each of the side grooves (18) and a nozzle (20) is fixedly connected to the bottom surface. A through groove (21) is provided on the movable plate (19) and is hingedly connected to a connecting plate (22). The connecting plate (22) extends out of the side grooves (18) and is hingedly connected to the circular plate (17).

6. The Ninghong Camellia chrysantha flowering process based on Eurotium cristatum according to claim 5, characterized in that: The inoculation control component (12) includes a fixed shell (23), the fixed shell (23) is fixedly connected to the top surface of the top plate (7), the side wall of the fixed shell (23) is fixedly connected to a circular tube (24) that passes through the feed shell (13), the inner cavity of the fixed shell (23) is provided with a push plate (25), the top surface of the push plate (25) is fixedly connected to a movable rod (26), the movable rod (26) passes through the fixed shell (23) and the top surface of the circular shell (1) and is fixedly connected to the same annular plate (27), the annular plate (27) is fixedly connected to an electric push rod (28), and the electric push rod (28) is fixedly connected to the circular shell (1).

7. The Ninghong Camellia chrysantha flowering process based on Eurotium cristatum according to claim 6, characterized in that: A transparent plate (29) and a handle (30) are fixedly connected to the door panel (2).

8. The Ninghong Camellia chrysantha flowering process based on Eurotium cristatum according to claim 1, characterized in that: In step S4, hot air drying is adopted to evenly spread the tea leaves on the tray of the drying equipment with a thickness of 2-3 cm. The hot air temperature is set to 50° C.-60° C. and the wind speed is set to 0.5-1 m / s. The drying time is adjusted according to the moisture content of the tea leaves and the batch size, and is 4-6 hours, until the moisture content of the tea leaves is less than 8%.

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