Nutritional supplement composition for maintaining mitochondrial function and preparation method thereof

Abstract

The invention belongs to the field of preparation of nutritional supplements, and particularly relates to a nutritional supplement composition for maintaining a mitochondrial function and a preparation method of the nutritional supplement composition. The preparation method comprises the following steps: cross-linking through non-covalent interaction to form a compound with a net structure, adding coenzyme Q10, L-carnitine and sodium caprylate to obtain a carrier material, fully adsorbing a mitochondrial function improving mixture, a plant extraction composition and an adjusting component, and freeze-drying to obtain the mitochondrial maintenance nutritional composition capable of efficiently exerting the mitochondrial maintenance effect. A compound which has a three-dimensional network structure and can form a film after being dried is mixed with a repair promoting composition and a fermented product, and freeze spray drying is performed to obtain the auxiliary mitochondrial function improving agent which can remarkably promote absorption of mitochondria and small intestines to effective components; the nutritional supplement composition with the function of maintaining mitochondria, which is prepared by uniformly dispersing the nutritional composition for maintaining mitochondria in the mixed components, has a remarkable maintenance effect and does not cause gastrointestinal discomfort after being used for a long time.

Description

Technical Field

[0001] The present invention belongs to the field of nutritional supplement preparation, and specifically relates to a nutritional supplement composition for maintaining mitochondrial function and a preparation method thereof. Background Art

[0002] Studies have shown that mitochondria are closely related to a variety of health problems such as chronic fatigue, sleep disorders, and cell aging. Currently, most supplements on the market are single-ingredient supplements, lacking complex formulas designed for mitochondrial maintenance, and have limited effects.

[0003] Mitochondrial maintenance agents are a class of compositions or drugs that improve mitochondrial structural integrity, enhance ATP synthesis efficiency, and reduce oxidative stress damage through antioxidant, energy metabolism support, or targeted delivery technologies. These agents, often in the form of soft capsules, nanoparticles, or creams, can slow cellular energy decline and maintain cellular homeostasis in patients with aging, metabolic diseases, or mitochondrial dysfunction, such as neurodegenerative diseases and chronic fatigue.

[0004] Currently, the preparation technology of existing compositions for maintaining mitochondrial function has the following problems: First, the maintenance effect of existing compositions for maintaining mitochondrial function on mitochondria is limited, the maintenance effect is not significant, and the effect is unstable; Second, due to membrane permeability limitations, existing compositions for maintaining mitochondrial function are difficult to accurately enter mitochondria and have insufficient bioavailability; Third, long-term use of existing compositions for maintaining mitochondrial function will cause gastrointestinal irritation. Summary of the Invention

[0005] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a nutritional supplement composition for maintaining mitochondrial function and a preparation method thereof. In order to solve the problems that the existing compositions for maintaining mitochondrial function have no significant maintenance effect, unstable effect, difficulty in accurately entering the mitochondria, low bioavailability, and long-term use will cause gastrointestinal irritation, the present invention uses a mitochondrial maintenance nutritional composition, an auxiliary mitochondrial function improvement agent, and an active and mild component to mix. The prepared nutritional supplement composition for maintaining mitochondrial function has a significant maintenance effect, can efficiently enter the mitochondria, has high bioavailability, will not cause gastrointestinal discomfort after long-term use, and can exert its effect for a long time.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: the present invention provides a nutritional supplement composition for maintaining mitochondrial function, wherein the raw materials for preparing the nutritional supplement composition for maintaining mitochondrial function specifically include the following components in parts by weight: 18-22 parts of mitochondrial nourishing nutritional composition, 11-13 parts of auxiliary agent for improving mitochondrial function, 1.5-2 parts of α-lipoic acid, 1.1-1.3 parts of hydroxytyrosol, 2.5-3.5 parts of cod collagen peptide, 1.2-1.4 parts of calcium pantothenate, and 0.8-1.2 parts of N-acetyl-L-cysteine.

[0007] Preferably, the raw materials for preparing the mitochondrial maintenance nutritional composition include the following components in parts by weight: 4-7 parts of fish collagen tripeptide, 1.1-1.4 parts of epigallocatechin gallate, 1.1-1.7 parts of nicotinamide riboside, 2.5-3.2 parts of grape seeds, 1.8-2.3 parts of pine bark, 2.2-2.4 parts of cyperus rotundus, 0.8-1.1 parts of selenomethionine, 0.7-0.9 parts of soybeans, 2.4-2.8 parts of black walnut kernels, 1.5-1.8 parts of saffron extract, 1.1-1.4 parts of verbena extract, 0.5-0.8 parts of disodium edetate, 0.7-1.2 parts of licorice extract, 0.6-0.9 parts of chitosan, 1.2-1.5 parts of pectin, and coenzyme Q10. 0.5-0.8 parts, L-carnitine 0.3-0.6 parts, sodium octanoate 0.7-0.9 parts.

[0008] Preferably, the raw materials for preparing the agent for assisting in improving mitochondrial function include the following components in parts by weight: 2.2-2.6 parts of ginseng extract, 1.1-1.3 parts of spermidine, 0.4-0.8 parts of asiaticoside, 2.2-2.5 parts of guava leaves, 2.4-2.7 parts of red beans, 2.5-2.8 parts of eucommia bark, 3.2-3.6 parts of thyme, 1.1-1.3 parts of mixed probiotics, 2-2.5 parts of milk thistle seed oil, 0.8-1.2 parts of carvacrol, 1.1-1.3 parts of limonene, 0.3-0.6 parts of catechins, 0.4-0.7 parts of proanthocyanidins, 1.2-1.4 parts of emblica extract, and 0.5-0.8 parts of resveratrol; The mixed probiotics are composed of Lactobacillus plantarum, Lactobacillus rhamnosus, and Bifidobacterium longum subspecies longum, with a weight ratio of 0.9:1.1:1. The Lactobacillus plantarum was purchased from the China General Microorganism Culture Collection Center with a preservation number of CGMCC1.12732, the Lactobacillus rhamnosus was purchased from the China General Microorganism Culture Collection Center with a preservation number of CGMCC1.577, and the Bifidobacterium longum subspecies longum was purchased from the China General Microorganism Culture Collection Center with a preservation number of CGMCC1.5052.

[0009] Preferably, the method for preparing the mitochondrial nourishing nutrient composition comprises the following steps: S1. Place fish collagen tripeptide, epigallocatechin gallate, and nicotinamide riboside in a stirrer with a power of 1.5-1.8 kW, stir at a temperature of 25-33° C., a stirring speed of 300-320 r / min, and a stirring time of 10-15 min to obtain a mixture for improving mitochondrial function; S2. Put grape seeds, pine bark, and cyperus rotundus into a grinder with a power of 1.5-1.8 kW, the grinding temperature is 23-28° C., the grinding time is 15-20 min, and the grinding speed is 3500-4500 r / min. After grinding, put them into an ultrasonic extractor with a power of 1.5 kW, the ultrasonic frequency is 20 kHz, the ultrasonic extraction temperature is 40° C., and the ultrasonic extraction time is 30 min. After ultrasonic extraction, put them into a stirrer with a power of 1.5-1.8 kW, the stirring temperature is 25-33° C., the stirring speed is 300-320 r / min, and the stirring time is 10-15 min to obtain a plant extract composition; S3. Put soybeans and black walnut kernels into a refiner with a power of 1.2-1.6 kW, the refining temperature is 65-75° C., the refining time is 35-40 min, the refining speed is 13000-16000 r / min, and the soybeans and black walnut kernels are put into a stirrer with a power of 1.5-1.8 kW, the stirring temperature is 25-33° C., the stirring speed is 300-320 r / min, and the stirring time is 10-15 min to obtain an adjusting component; S4. Place disodium EDTA, licorice extract, chitosan, pectin, and 2% acetic acid solution in a reactor with a power of 2.5-3.2 kW, the reaction temperature is 50-60°C, the reaction time is 4 h, and the reaction speed is 300 r / min. The reaction is carried out, and coenzyme Q10, L-carnitine, and sodium octanoate are added. The mixture is placed in a stirrer with a power of 1.5-1.8 kW, the stirring temperature is 25-28°C, the stirring speed is 300-500 r / min, and the stirring time is 10-20 min to obtain a carrier material; S5. Place the mixture for improving mitochondrial function prepared by S1, the plant extract composition prepared by S2, and the regulating component prepared by S3 into the carrier material prepared by S4, stir at a temperature of 25-33°C, a stirring speed of 300-320 r / min, and a stirring time of 20-25 min, stir and mix, fully adsorb, place in a freeze dryer with a power of 2.3-2.8 kW, freeze-dry at a temperature of -40°C, freeze-dry for 4 hours, and freeze-dry to obtain a mitochondrial maintenance nutrient composition.

[0010] Furthermore, in S2, the material-liquid ratio of the grape seeds to the 70% ethanol solution is 1:2.5 g / mL.

[0011] Furthermore, in S4, the mass fraction of the disodium edetate in the 2% acetic acid solution is 4%.

[0012] Preferably, the method for preparing the agent for assisting in improving mitochondrial function comprises the following steps: L1. Place ginseng extract, spermidine, and asiaticoside in a stirrer with a power of 1.5-1.8 kW, stir at a temperature of 25-28° C., a stirring speed of 300-320 r / min, and a stirring time of 10-15 min to obtain a repair-promoting composition; L2. Put guava leaves, red beans, eucommia ulmoides, and thyme into a grinder with a power of 1.5-1.8 kW, the grinding temperature is 23-28°C, the grinding time is 15-20 min, and the grinding speed is 3500-4500 r / min. After grinding, put them into a fermentation tank with a power of 1.8-2.5 kW with mixed probiotics and ultrapure water, and stir and ferment at a temperature of 37°C, a speed of 300-500 r / min, and a fermentation time of 3-5 d. After fermentation, add milk thistle seed oil, carvacrol, and limonene, stir at a temperature of 25-30°C, stir for 30 min, and stir at a speed of 600 r / min to obtain a fermented product; L3. Place catechins, proanthocyanidins, emblica extract, resveratrol, and 50% ethanol solution in a reactor with a power of 2.5-3.2 kW, react at a temperature of 50-60°C, a reaction time of 1-2 h, and a reaction speed of 200 r / min to obtain a complex suspension; L4. Put the pro-repair composition prepared by L1 and the fermentation product prepared by L2 into the complex suspension prepared by L3, stir at a temperature of 25-33°C, a stirring speed of 300-320 r / min, and a stirring time of 10-15 min. Stir and mix, put into a vacuum freeze nano spray dryer with a power of 2.3-2.8 kW, a vacuum degree of -0.05 MPa, a freeze nano spray drying temperature of -40°C, a freeze nano spray drying particle size of 100 nm, a freeze nano spray drying time of 2.5-3 h, and freeze spray drying to obtain an agent that assists in improving mitochondrial function.

[0013] Furthermore, in L2, the material-liquid ratio of the mixed probiotics to ultrapure water is 1:100 g / mL.

[0014] Furthermore, in L3, the mass fraction of the catechin in the 50% ethanol solution is 2%.

[0015] The present invention also provides a method for preparing a nutritional supplement composition for maintaining mitochondrial function, which specifically comprises the following steps: Step 1: Place α-lipoic acid, hydroxytyrosol, cod collagen peptide, calcium pantothenate, and N-acetyl-L-cysteine ​​into a 1.8 kW stirrer at a stirring temperature of 25-33° C., a stirring speed of 300-320 r / min, and a stirring time of 10-15 min to obtain a mild component that maintains activity; Step 2: Add the auxiliary agent for improving mitochondrial function into the active and mild component prepared in step 1, stir at a temperature of 25-33° C., a stirring speed of 300-320 r / min, and a stirring time of 10-15 min to obtain a mixed component; Step 3: Place the mitochondrial nutritional composition and the mixed components prepared in step 2 into an ultrasonic stirrer with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min to uniformly disperse them to obtain a nutritional supplement composition for maintaining mitochondrial function.

[0016] The beneficial effects achieved by the present invention are as follows: The present invention adopts fish collagen tripeptide, epigallocatechin gallate, and nicotinamide riboside to obtain a mixture for improving mitochondrial function, which synergistically improves mitochondrial function and cellular energy metabolism through multiple mechanisms such as activating mitochondrial generation, repairing the electron transport chain, increasing NAD⁺ levels, and regulating mitochondrial dynamics and autophagy. Grape seeds, pine bark, and cyperus rotundus are crushed, extracted with a 70% ethanol solution, and mixed with selenomethionine to obtain a plant extract composition that can efficiently remove hydrogen peroxide and lipid peroxides, block free radical chain reactions, enhance the activity of the antioxidant enzyme system, and indirectly reduce free radical accumulation. Soybeans and black walnut kernels are ground into pulp and mixed with saffron extract and verbena extract to obtain a regulating The plant protein, branched-chain amino acids, phosphatidylserine, and α-linolenic acid contained in the section components can accelerate muscle glycogen resynthesis, reduce anxiety-related inflammatory factors, increase 5-hydroxytryptamine levels, and improve mood and sleep. Disodium EDTA, licorice extract, chitosan, pectin, and 2% acetic acid solution are mixed and cross-linked through non-covalent interactions to form a complex with a network structure. Coenzyme Q10, L-carnitine, and sodium octanoate are added and mixed. The resulting carrier material can directly pass through the outer mitochondrial membrane without the need for a carnitine transport system, quickly enter the mitochondrial matrix for β-oxidation, and can attach to the surface of the small intestinal villi to directly improve the mitochondrial uptake and utilization efficiency of active ingredients. The carrier material The mitochondrial function-improving mixture, plant extract composition, and regulating component are fully adsorbed and freeze-dried to obtain a mitochondrial maintenance nutrient composition that can effectively exert the effect of maintaining mitochondria; the ginseng extract, spermidine, and asiaticoside are mixed to obtain a repair-promoting composition that reduces the accumulation of reactive oxygen species, protects mitochondrial function, reduces oxidative stress levels, slows down the aging process of cells, and removes damaged organelles by inducing autophagy. It restores the regeneration capacity of stem cells, restores mitochondrial protein synthesis, improves mitochondrial dysfunction, protects mitochondrial membrane lipids from oxidation, maintains their structural integrity, and significantly enhances the self-repair ability and anti-aging potential of cells. Guava leaves, red beans, eucommia ulmoides, and thyme are crushed After synergistic fermentation by Lactobacillus plantarum, Lactobacillus rhamnosus, and Bifidobacterium longum subspecies longum, the fermentation products can promote mitochondrial biogenesis, enhance mitochondrial antioxidant capacity, remove damaged mitochondria, and delay cell energy decline in multiple dimensions. Catechins, proanthocyanidins, emblica extract, resveratrol, and 50% ethanol solution are mixed and reacted, and self-assembled into a three-dimensional network structure through non-covalent interaction. After drying, they can form a flexible, transparent, functional film with both structural stability and biological activity. The film-forming complex is mixed with the pro-repair composition and the fermentation product, and freeze-spray-dried to obtain an auxiliary mitochondrial function-improving agent that can significantly promote the absorption efficiency and functional utilization of effective components by mitochondria and small intestine.Alpha-lipoic acid, hydroxytyrosol, cod collagen peptide, calcium pantothenate, and N-acetyl-L-cysteine ​​are mixed to obtain a mild component that maintains activity, which can protect the stability of the effective ingredients, maximize the efficacy, and minimize gastrointestinal irritation. The mitochondrial maintenance nutritional composition is evenly dispersed in the mixed components. The nutritional supplement composition for maintaining mitochondrial function has a significant maintenance effect and can effectively solve the problems of fatigue, metabolic decline, and aging caused by mitochondrial functional degeneration. Through the combination of multiple nutrients, the mitochondrial energy production, antioxidant and repair capabilities are enhanced, and the mitochondrial energy production, antioxidant and repair capabilities are enhanced. The mitochondria can be efficiently entered into the mitochondria, and the bioavailability is high. Long-term use will not cause gastrointestinal discomfort and can exert a long-lasting effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the present invention or the technical solutions in the prior art, the following will be described in a clear and easy-to-understand manner with reference to the accompanying drawings. Obviously, the drawings described below are only for the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0018] Figure 1 This is a graph showing the difference in blood flow in the mouse auricle microcirculation described in Experimental Example 1 of the present invention; Figure 2 This is a graph showing the number of times mice fell off the wheel as described in Experimental Example 1 of the present invention; Figure 3 This is a graph showing the difference in blood flow in the microcirculation of the mouse auricle after storage as described in Experimental Example 2 of the present invention; Figure 4 This is a graph showing the number of times mice fell off the wheel after storage as described in Experimental Example 2 of the present invention. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0020] Unless otherwise defined, all technical and scientific terms used herein have the same meanings as those familiar to those skilled in the art. Furthermore, any methods and materials similar or equivalent to those described herein can be applied to the present invention. The preferred embodiments and materials described herein are for illustrative purposes only and are not intended to limit the scope of this application.

[0021] The experimental methods in the following examples, unless otherwise specified, are conventional methods; the test materials and test strains used in the following examples, unless otherwise specified, are purchased from commercial channels.

[0022] Example 1: This example provides a nutritional supplement composition for maintaining mitochondrial function and a preparation method thereof. The nutritional supplement composition for maintaining mitochondrial function comprises the following components in parts by weight: 18 parts of mitochondrial maintenance nutritional composition, 11 parts of auxiliary mitochondrial function improvement agent, 1.5 parts of α-lipoic acid, 1.1 parts of hydroxytyrosol, 2.5 parts of cod collagen peptide, 1.2 parts of calcium pantothenate, and 0.8 parts of N-acetyl-L-cysteine.

[0023] The raw materials for preparing the mitochondrial maintenance nutritional composition include the following components in parts by weight: 4 parts of fish collagen tripeptide, 1.1 parts of epigallocatechin gallate, 1.1 parts of nicotinamide riboside, 2.5 parts of grape seeds, 1.8 parts of pine bark, 2.2 parts of cyperus rotundus, 0.8 parts of selenomethionine, 0.7 parts of soybeans, 2.4 parts of black walnut kernels, 1.5 parts of saffron extract, 1.1 parts of verbena extract, 0.5 parts of disodium edetate, 0.7 parts of licorice extract, 0.6 parts of chitosan, 1.2 parts of pectin, 0.5 parts of coenzyme Q10, 0.3 parts of L-carnitine, and 0.7 parts of sodium octanoate.

[0024] The raw materials for preparing the auxiliary agent for improving mitochondrial function include the following components in parts by weight: 2.2 parts of ginseng extract, 1.1 parts of spermidine, 0.4 parts of asiaticoside, 2.2 parts of guava leaves, 2.4 parts of red beans, 2.5 parts of eucommia bark, 3.2 parts of thyme, 1.1 parts of mixed probiotics, 2 parts of milk thistle seed oil, 0.8 parts of carvacrol, 1.1 parts of limonene, 0.3 parts of catechins, 0.4 parts of proanthocyanidins, 1.2 parts of emblica extract, and 0.5 parts of resveratrol; The mixed probiotics are composed of Lactobacillus plantarum, Lactobacillus rhamnosus, and Bifidobacterium longum subspecies longum, with a weight ratio of 0.9:1.1:1. The Lactobacillus plantarum was purchased from the China General Microorganism Culture Collection Center with a preservation number of CGMCC1.12732, the Lactobacillus rhamnosus was purchased from the China General Microorganism Culture Collection Center with a preservation number of CGMCC1.577, and the Bifidobacterium longum subspecies longum was purchased from the China General Microorganism Culture Collection Center with a preservation number of CGMCC1.5052.

[0025] The preparation method of the mitochondrial maintenance nutritional composition specifically comprises the following steps: S1. Place fish collagen tripeptide, epigallocatechin gallate, and nicotinamide riboside in a 1.5 kW stirrer at a stirring temperature of 25°C and a stirring speed of 300 r / min for 10 min to obtain a mixture for improving mitochondrial function; S2. Put grape seeds, pine bark, and cyperus rotundus into a grinder with a power of 1.5 kW, the grinding temperature is 23° C., the grinding time is 15 min, and the grinding speed is 3500 r / min. After grinding, put them into an ultrasonic extractor with a power of 1.5 kW, the ultrasonic frequency is 20 kHz, the ultrasonic extraction temperature is 40° C., and the ultrasonic extraction time is 30 min. After ultrasonic extraction, put them into a stirrer with a power of 1.5 kW, the stirring temperature is 25° C., the stirring speed is 300 r / min, and the stirring time is 10 min to obtain a plant extract composition; S3. Put soybeans and black walnut kernels into a 1.2 kW refiner at a refining temperature of 65° C., a refining time of 35 min, and a refining speed of 13,000 r / min for refining. Then, put them into a 1.5 kW stirrer with saffron extract and verbena extract at a stirring temperature of 25° C., a stirring speed of 300 r / min, and a stirring time of 10 min to obtain a regulating component; S4. Disodium EDTA, licorice extract, chitosan, pectin, and 2% acetic acid solution were placed in a reactor with a power of 2.5 kW, the reaction temperature was 50°C, the reaction time was 4 h, and the reaction speed was 300 r / min. Coenzyme Q10, L-carnitine, and sodium octanoate were added, and the mixture was placed in a stirrer with a power of 1.5 kW, the stirring temperature was 25°C, the stirring speed was 300 r / min, and the stirring time was 10 min to obtain a carrier material. S5. Place the mixture for improving mitochondrial function prepared by S1, the plant extract composition prepared by S2, and the regulating component prepared by S3 into the carrier material prepared by S4, stir at a temperature of 25°C, a stirring speed of 300 r / min, and a stirring time of 20 min, stir and mix, fully adsorb, place in a freeze dryer with a power of 2.3 kW, freeze-dry at a temperature of -40°C, freeze-dry for 4 h, and freeze-dry to obtain a mitochondrial maintenance nutrient composition.

[0026] In S2, the material-liquid ratio of the grape seeds to the 70% ethanol solution is 1:2.5 g / mL.

[0027] In S4, the mass fraction of disodium edetate in the 2% acetic acid solution is 4%.

[0028] The preparation method of the agent for assisting in improving mitochondrial function specifically comprises the following steps: L1. Place ginseng extract, spermidine, and asiaticoside in a 1.5 kW stirrer at a stirring temperature of 25° C. and a stirring speed of 300 r / min for 10 min to obtain a repair-promoting composition; L2. Put guava leaves, red beans, eucommia ulmoides, and thyme into a grinder with a power of 1.5 kW, the grinding temperature is 23°C, the grinding time is 15 min, and the grinding speed is 3500 r / min. After grinding, put them into a fermentation tank with a power of 1.8 kW with mixed probiotics and ultrapure water, and stir and ferment at a temperature of 37°C, a speed of 300 r / min, and a fermentation time of 3 days. After fermentation, add milk thistle seed oil, carvacrol, and limonene, stir at a temperature of 25°C, a time of 30 min, and a speed of 600 r / min to obtain a fermentation product; L3. Place catechins, proanthocyanidins, emblica extract, resveratrol, and 50% ethanol solution in a 2.5 kW reactor, set the reaction temperature to 50°C, the reaction time to 1 h, and the reaction speed to 200 r / min to obtain a complex suspension; L4. Put the pro-repair composition prepared by L1 and the fermentation product prepared by L2 into the complex suspension prepared by L3, stir at a temperature of 25°C, a stirring speed of 300 r / min, and a stirring time of 10 min. Stir and mix, put into a vacuum freeze-nano spray dryer with a power of 2.3 kW, a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 2.5 h. Freeze-spray drying to obtain an agent that assists in improving mitochondrial function.

[0029] In L2, the material-liquid ratio of the mixed probiotics to ultrapure water was 1:100 g / mL.

[0030] In L3, the mass fraction of the catechin in the 50% ethanol solution is 2%.

[0031] This embodiment also provides a method for preparing a nutritional supplement composition for maintaining mitochondrial function, which specifically comprises the following steps: Step 1: Place α-lipoic acid, hydroxytyrosol, cod collagen peptide, calcium pantothenate, and N-acetyl-L-cysteine ​​into a 1.8 kW stirrer at a stirring temperature of 25° C., a stirring speed of 300 r / min, and a stirring time of 10 min to obtain a mild component that maintains activity; Step 2: Add the auxiliary agent for improving mitochondrial function into the active and mild component prepared in step 1, stir at a temperature of 25° C., a stirring speed of 300 r / min, and a stirring time of 10 min to obtain a mixed component; Step 3: Place the mitochondrial nutritional composition and the mixed components prepared in step 2 into an ultrasonic stirrer with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min to uniformly disperse them to obtain a nutritional supplement composition for maintaining mitochondrial function.

[0032] Example 2: This example provides a nutritional supplement composition for maintaining mitochondrial function and a preparation method thereof. The nutritional supplement composition for maintaining mitochondrial function comprises the following components in parts by weight: 20 parts of mitochondrial maintenance nutritional composition, 12 parts of auxiliary agent for improving mitochondrial function, 1.8 parts of α-lipoic acid, 1.2 parts of hydroxytyrosol, 3 parts of cod collagen peptide, 1.3 parts of calcium pantothenate, and 1.1 parts of N-acetyl-L-cysteine.

[0033] The raw materials for preparing the mitochondrial maintenance nutritional composition include the following components in parts by weight: 6 parts of fish collagen tripeptide, 1.3 parts of epigallocatechin gallate, 1.5 parts of nicotinamide riboside, 2.8 parts of grape seeds, 2.1 parts of pine bark, 2.3 parts of cyperus rotundus, 0.9 parts of selenomethionine, 0.8 parts of soybeans, 2.7 parts of black walnut kernels, 1.6 parts of saffron extract, 1.2 parts of verbena extract, 0.6 parts of disodium edetate, 0.9 parts of licorice extract, 0.8 parts of chitosan, 1.4 parts of pectin, 0.7 parts of coenzyme Q10, 0.5 parts of L-carnitine, and 0.8 parts of sodium octanoate.

[0034] The raw materials for preparing the auxiliary agent for improving mitochondrial function include the following components in parts by weight: 2.4 parts of ginseng extract, 1.2 parts of spermidine, 0.6 parts of asiaticoside, 2.3 parts of guava leaves, 2.6 parts of red beans, 2.7 parts of eucommia bark, 3.5 parts of thyme, 1.2 parts of mixed probiotics, 2.3 parts of milk thistle seed oil, 1.1 parts of carvacrol, 1.2 parts of limonene, 0.5 parts of catechins, 0.6 parts of proanthocyanidins, 1.3 parts of emblica extract, and 0.7 parts of resveratrol; The mixed probiotics are composed of Lactobacillus plantarum, Lactobacillus rhamnosus, and Bifidobacterium longum subspecies longum, with a weight ratio of 0.9:1.1:1. The Lactobacillus plantarum was purchased from the China General Microorganism Culture Collection Center with a preservation number of CGMCC1.12732, the Lactobacillus rhamnosus was purchased from the China General Microorganism Culture Collection Center with a preservation number of CGMCC1.577, and the Bifidobacterium longum subspecies longum was purchased from the China General Microorganism Culture Collection Center with a preservation number of CGMCC1.5052.

[0035] The preparation method of the mitochondrial maintenance nutritional composition specifically comprises the following steps: S1. Place fish collagen tripeptide, epigallocatechin gallate, and nicotinamide riboside in a 1.6 kW stirrer at a stirring temperature of 30°C, a stirring speed of 310 r / min, and a stirring time of 13 min to obtain a mixture for improving mitochondrial function; S2. Put grape seeds, pine bark, and cyperus rotundus into a grinder with a power of 1.7 kW, a grinding temperature of 26° C., a grinding time of 18 min, and a grinding speed of 4000 r / min. After grinding, put them into an ultrasonic extractor with a power of 1.5 kW, an ultrasonic frequency of 20 kHz, an ultrasonic extraction temperature of 40° C., and an ultrasonic extraction time of 30 min with selenomethionine, put them into a stirrer with a power of 1.7 kW, a stirring temperature of 30° C., a stirring speed of 310 r / min, and a stirring time of 13 min to obtain a plant extract composition; S3. Put soybeans and black walnut kernels into a 1.5 kW refiner at a refining temperature of 70° C., a refining time of 38 min, and a refining speed of 15,000 r / min for refining. Then put them into a 1.6 kW stirrer with saffron extract and verbena extract at a stirring temperature of 32° C., a stirring speed of 310 r / min, and a stirring time of 13 min to obtain a regulating component; S4. Disodium EDTA, licorice extract, chitosan, pectin, and 2% acetic acid solution were placed in a reactor with a power of 2.8 kW, the reaction temperature was 55°C, the reaction time was 4 h, and the reaction speed was 300 r / min. Coenzyme Q10, L-carnitine, and sodium octanoate were added, and the mixture was placed in a stirrer with a power of 1.6 kW, the stirring temperature was 27°C, the stirring speed was 400 r / min, and the stirring time was 15 min to obtain a carrier material. S5. Place the mixture for improving mitochondrial function prepared by S1, the plant extract composition prepared by S2, and the regulating component prepared by S3 into the carrier material prepared by S4, stir at a temperature of 30°C, a stirring speed of 310 r / min, and a stirring time of 23 min, stir and mix, fully adsorb, place in a freeze dryer with a power of 2.6 kW, freeze-dry at a temperature of -40°C, freeze-dry for 4 h, and freeze-dry to obtain a mitochondrial maintenance nutrient composition.

[0036] In S2, the material-liquid ratio of the grape seeds to the 70% ethanol solution is 1:2.5 g / mL.

[0037] In S4, the mass fraction of disodium edetate in the 2% acetic acid solution is 4%.

[0038] The preparation method of the agent for assisting in improving mitochondrial function specifically comprises the following steps: L1. Place ginseng extract, spermidine, and asiaticoside in a 1.6 kW stirrer at a stirring temperature of 27° C., a stirring speed of 310 r / min, and a stirring time of 14 min to mix to obtain a repair-promoting composition; L2. Put guava leaves, red beans, eucommia ulmoides, and thyme into a grinder with a power of 1.6 kW, the grinding temperature is 27°C, the grinding time is 18 min, and the grinding speed is 4000 r / min. After grinding, put them into a fermentation tank with a power of 2.3 kW with mixed probiotics and ultrapure water, and stir and ferment at a temperature of 37°C, a speed of 400 r / min, and a fermentation time of 4 days. After fermentation, add milk thistle seed oil, carvacrol, and limonene, stir at a temperature of 28°C, a time of 30 min, and a speed of 600 r / min to obtain a fermentation product; L3, placing catechins, proanthocyanidins, emblica extract, resveratrol, and 50% ethanol solution in a 3.1 kW reactor, reacting at 55°C for 1.5 h and at a speed of 200 r / min to obtain a complex suspension; L4. Put the pro-repair composition prepared by L1 and the fermentation product prepared by L2 into the complex suspension prepared by L3, stir at a temperature of 28°C, a stirring speed of 310 r / min, and a stirring time of 14 min. Stir and mix, put into a vacuum freeze-nano spray dryer with a power of 2.7 kW, a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 2.8 h. Freeze-spray drying to obtain an agent that assists in improving mitochondrial function.

[0039] In L2, the material-liquid ratio of the mixed probiotics to ultrapure water was 1:100 g / mL.

[0040] In L3, the mass fraction of the catechin in the 50% ethanol solution is 2%.

[0041] This embodiment also provides a method for preparing a nutritional supplement composition for maintaining mitochondrial function, which specifically comprises the following steps: Step 1: Place α-lipoic acid, hydroxytyrosol, cod collagen peptide, calcium pantothenate, and N-acetyl-L-cysteine ​​into a 1.8 kW stirrer at a stirring temperature of 30° C., a stirring speed of 310 r / min, and a stirring time of 12 minutes to mix to obtain an active and mild component; Step 2: Add the auxiliary agent for improving mitochondrial function into the active and mild component prepared in step 1, stir at a temperature of 31° C., a stirring speed of 310 r / min, and a stirring time of 13 min to obtain a mixed component; Step 3: Place the mitochondrial nutritional composition and the mixed components prepared in step 2 into an ultrasonic stirrer with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min to uniformly disperse them to obtain a nutritional supplement composition for maintaining mitochondrial function.

[0042] Example 3: This example provides a nutritional supplement composition for maintaining mitochondrial function and a preparation method thereof. The nutritional supplement composition for maintaining mitochondrial function comprises the following components in parts by weight: 22 parts of mitochondrial maintenance nutritional composition, 13 parts of auxiliary mitochondrial function improvement agent, 2 parts of α-lipoic acid, 1.3 parts of hydroxytyrosol, 3.5 parts of cod collagen peptide, 1.4 parts of calcium pantothenate, and 1.2 parts of N-acetyl-L-cysteine.

[0043] The raw materials for preparing the mitochondrial maintenance nutritional composition include the following components in parts by weight: 7 parts of fish collagen tripeptide, 1.4 parts of epigallocatechin gallate, 1.7 parts of nicotinamide riboside, 3.2 parts of grape seeds, 2.3 parts of pine bark, 2.4 parts of cyperus rotundus, 1.1 parts of selenomethionine, 0.9 parts of soybeans, 2.8 parts of black walnut kernels, 1.8 parts of saffron extract, 1.4 parts of verbena extract, 0.8 parts of disodium edetate, 1.2 parts of licorice extract, 0.9 parts of chitosan, 1.5 parts of pectin, 0.8 parts of coenzyme Q10, 0.6 parts of L-carnitine, and 0.9 parts of sodium octanoate.

[0044] The raw materials for preparing the auxiliary agent for improving mitochondrial function include the following components in parts by weight: 2.6 parts of ginseng extract, 1.3 parts of spermidine, 0.8 parts of asiaticoside, 2.5 parts of guava leaves, 2.7 parts of red beans, 2.8 parts of eucommia bark, 3.6 parts of thyme, 1.3 parts of mixed probiotics, 2.5 parts of milk thistle seed oil, 1.2 parts of carvacrol, 1.3 parts of limonene, 0.6 parts of catechins, 0.7 parts of proanthocyanidins, 1.4 parts of emblica extract, and 0.8 parts of resveratrol; The mixed probiotics are composed of Lactobacillus plantarum, Lactobacillus rhamnosus, and Bifidobacterium longum subspecies longum, with a weight ratio of 0.9:1.1:1. The Lactobacillus plantarum was purchased from the China General Microorganism Culture Collection Center with a preservation number of CGMCC1.12732, the Lactobacillus rhamnosus was purchased from the China General Microorganism Culture Collection Center with a preservation number of CGMCC1.577, and the Bifidobacterium longum subspecies longum was purchased from the China General Microorganism Culture Collection Center with a preservation number of CGMCC1.5052.

[0045] The preparation method of the mitochondrial maintenance nutritional composition specifically comprises the following steps: S1. Place fish collagen tripeptide, epigallocatechin gallate, and nicotinamide riboside in a 1.8 kW stirrer at a stirring temperature of 33°C, a stirring speed of 320 r / min, and a stirring time of 15 min to obtain a mixture for improving mitochondrial function; S2. Put grape seeds, pine bark, and cyperus rotundus into a grinder with a power of 1.8 kW, the grinding temperature is 28° C., the grinding time is 20 min, and the grinding speed is 4500 r / min. After grinding, put them into an ultrasonic extractor with a power of 1.5 kW, the ultrasonic frequency is 20 kHz, the ultrasonic extraction temperature is 40° C., and the ultrasonic extraction time is 30 min. After ultrasonic extraction, put them into a stirrer with a power of 1.8 kW, the stirring temperature is 33° C., the stirring speed is 320 r / min, and the stirring time is 15 min to obtain a plant extract composition; S3. Put soybeans and black walnut kernels into a 1.6 kW refiner at a refining temperature of 75° C., a refining time of 40 min, and a refining speed of 16,000 r / min for refining. Then put them into a 1.8 kW stirrer with saffron extract and verbena extract at a stirring temperature of 33° C., a stirring speed of 320 r / min, and a stirring time of 15 min to obtain a regulating component; S4. Disodium EDTA, licorice extract, chitosan, pectin, and 2% acetic acid solution were placed in a reactor with a power of 3.2 kW, the reaction temperature was 60°C, the reaction time was 4 h, and the reaction speed was 300 r / min. Coenzyme Q10, L-carnitine, and sodium octanoate were added, and the mixture was placed in a stirrer with a power of 1.8 kW, the stirring temperature was 28°C, the stirring speed was 500 r / min, and the stirring time was 20 min to obtain a carrier material. S5. The mixture for improving mitochondrial function prepared by S1, the plant extract composition prepared by S2, and the regulating component prepared by S3 are placed into the carrier material prepared by S4, with the stirring temperature at 33°C, the stirring speed at 320 r / min, and the stirring time at 25 min. Stir and mix, fully adsorb, and place in a freeze dryer with a power of 2.8 kW, the freeze drying temperature at -40°C, the freeze drying time at 4 h, and freeze drying to obtain a mitochondrial maintenance nutrient composition.

[0046] In S2, the material-liquid ratio of the grape seeds to the 70% ethanol solution is 1:2.5 g / mL.

[0047] In S4, the mass fraction of disodium edetate in the 2% acetic acid solution is 4%.

[0048] The preparation method of the agent for assisting in improving mitochondrial function specifically comprises the following steps: L1. Place ginseng extract, spermidine, and asiaticoside in a 1.8 kW stirrer at a stirring temperature of 28° C., a stirring speed of 320 r / min, and a stirring time of 15 min to mix to obtain a repair-promoting composition; L2. Put guava leaves, red beans, eucommia ulmoides, and thyme into a grinder with a power of 1.8 kW, the grinding temperature is 28°C, the grinding time is 20 min, and the grinding speed is 4500 r / min. After grinding, put them into a fermentation tank with a power of 2.5 kW with mixed probiotics and ultrapure water, and stir and ferment at a temperature of 37°C, a speed of 500 r / min, and a fermentation time of 5 days. After fermentation, add milk thistle seed oil, carvacrol, and limonene, stir at a temperature of 30°C, a time of 30 min, and a speed of 600 r / min to obtain a fermentation product; L3. Place catechins, proanthocyanidins, emblica extract, resveratrol, and 50% ethanol solution in a 3.2 kW reactor, set the reaction temperature to 60°C, the reaction time to 2 h, and the reaction speed to 200 r / min to obtain a complex suspension. L4. Put the pro-repair composition prepared by L1 and the fermentation product prepared by L2 into the complex suspension prepared by L3, stir at a temperature of 33°C, a stirring speed of 320 r / min, and a stirring time of 15 min. Stir and mix, put into a vacuum freeze-nano spray dryer with a power of 2.8 kW, a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 3 h. Freeze-spray drying to obtain an agent that assists in improving mitochondrial function.

[0049] In L2, the material-liquid ratio of the mixed probiotics to ultrapure water was 1:100 g / mL.

[0050] In L3, the mass fraction of the catechin in the 50% ethanol solution is 2%.

[0051] This embodiment also provides a method for preparing a nutritional supplement composition for maintaining mitochondrial function, which specifically comprises the following steps: Step 1: Place α-lipoic acid, hydroxytyrosol, cod collagen peptide, calcium pantothenate, and N-acetyl-L-cysteine ​​into a 1.8 kW stirrer at a stirring temperature of 33° C., a stirring speed of 320 r / min, and a stirring time of 15 minutes to mix and obtain an active and mild component; Step 2: Add the auxiliary agent for improving mitochondrial function into the active and mild component prepared in step 1, stir at a temperature of 33° C., a stirring speed of 320 r / min, and a stirring time of 15 min to obtain a mixed component; Step 3: Place the mitochondrial nutritional composition and the mixed components prepared in step 2 into an ultrasonic stirrer with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min to uniformly disperse them to obtain a nutritional supplement composition for maintaining mitochondrial function.

[0052] Comparative Example 1: This comparative example provides a nutritional composition for maintaining mitochondrial function and a preparation method thereof. The only difference from Example 1 is that the added nutritional composition for maintaining mitochondria does not contain a carrier material, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0053] Comparative Example 2: This comparative example provides a nutritional composition for maintaining mitochondrial function and a preparation method thereof. The only difference from Example 1 is that the added auxiliary agent for improving mitochondrial function does not contain a complex suspension, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0054] Experimental Example 1: Test to measure the effect of improving fatigue and enhancing metabolism.

[0055] The test steps for determining the fatigue-improving and metabolic-enhancing effects of the nutritional supplement compositions for maintaining mitochondrial function prepared in Examples 1-3 of the present invention are as follows: (1) Sixty 8-week-old SPF mice weighing 25 g were selected (purchased from Chengdu Dashuo Animal Co., Ltd.) and randomly divided into 6 groups of 10 mice each. The mice were housed in separate cages, kept clean and dry, at a temperature of 24°C and a humidity of 50%, with free access to food and water. (2) After the mice in step (1) are anesthetized, the microcirculatory blood flow (PU) of the mouse auricle is measured using a laser Doppler microcirculatory instrument, and the result is recorded as the initial microcirculatory blood flow of the mouse auricle; (3) The nutritional supplement composition for maintaining mitochondrial function prepared in Example 1-3 was fed to the mice in Group 1-3 by gavage, with each mouse receiving 2.5 mg per day. The nutritional supplement composition for maintaining mitochondrial function prepared in Comparative Example 1-2 was fed to the mice in Group 1-2 by gavage, with each mouse receiving 2.5 mg per day. The commercially available mitochondrial maintenance agent (purchased from Guangzhou Limeikang Biotechnology Co., Ltd.) was fed to the mice in Group 1-3 by gavage, with each mouse receiving 2.5 mg per day. Two hours after the mice in Groups 1-3, Group 1-2 and Control were fed with the nutritional supplement composition for maintaining mitochondrial function prepared in Example 1-3, the nutritional supplement composition for maintaining mitochondrial function prepared in Comparative Example 1-2 and the commercially available mitochondrial maintenance agent in Control, the blood flow of the mice was measured and the data was recorded. The difference in the microcirculation blood flow of the mice's auricle was calculated according to the formula: the difference in the microcirculation blood flow of the mice's auricle = the microcirculation blood flow of the mice's auricle after administration - the initial microcirculation blood flow of the mice's auricle; (4) Sixty 8-week-old SPF mice weighing 25 g were selected (purchased from Chengdu Dashuo Animal Co., Ltd.) and randomly divided into 6 groups of 10 mice each. The mice were housed in separate cages, kept clean and dry, at a temperature of 24°C and a humidity of 50%, with free access to food and water. (5) Place the mouse in step (4) on a roller with a rotation speed of 18 r / min and start pedaling. Record the number of times the mouse falls off the wheel due to muscle fatigue within 20 minutes, which is recorded as the initial number of wheel falls. (6) On the second day, the nutritional supplement composition for maintaining mitochondrial function prepared in Example 1-3 was fed to the mice in Group 1-3 at a rate of 2.5 mg per day. The nutritional supplement composition for maintaining mitochondrial function prepared in Example 1-2 was fed to the mice in Group 1-2 at a rate of 2.5 mg per day. The commercially available mitochondrial maintenance agent (purchased from Guangzhou Limeikang Biotechnology Co., Ltd.) was fed to the mice in the control group at a rate of 2.5 mg per day. Three hours after feeding, the mice in each group were placed on a rolling wheel with a rotation speed of 18 r / min to start pedaling. The number of times the mice fell off the wheel due to muscle fatigue within 20 minutes was recorded as the number of times the wheel fell off after taking the medicine.

[0056] Result analysis: Figure 1This is a graph showing the difference in microcirculatory blood flow in the auricle of the mice described in Experimental Example 1 of the present invention. As shown in the figure, the mice in groups 1 to 3 of Examples were fed with the nutritional supplement composition for maintaining mitochondrial function prepared in Examples 1 to 3, and 2.5 mg was fed to each mouse every day for 2 hours. The differences in auricular microcirculation PU of the mice in groups 1 to 3 of Examples were 40.52, 41.64, and 43.41, respectively. The mice in groups 1 to 2 of Comparative Examples were fed with the nutritional composition for maintaining mitochondrial function prepared in Comparative Examples 1 to 2, and 2.5 mg was fed to each mouse every day for 2 hours. The differences in auricular microcirculation PU of the mice in groups 1 to 2 of Comparative Examples were 21.54 and 33.38, respectively. The mice in the control group were fed with the commercially available mitochondrial curing agent of the control group, and 2.5 mg was fed to each mouse every day for 2 hours. The difference in auricular microcirculation PU of the mice in the control group was 15.53. Figure 2 This is a result graph of the number of times the mice fell off the wheel as described in Experimental Example 1 of the present invention. As shown in the figure, after the mice in Example 1-3 groups were fed with the nutritional supplement composition for maintaining mitochondrial function prepared in Example 1-3 for 3 hours, they started to pedal on a roller with a speed of 18 r / min, and the number of falls within 20 minutes decreased significantly. After the mice in the control group were fed with the commercially available mitochondrial maintenance agent for 3 hours, they started to pedal on a roller with a speed of 18 r / min, and the difference in the number of falls within 20 minutes was basically small. This shows that the nutritional supplement composition for maintaining mitochondrial function prepared by the present invention has the effects of promoting blood circulation and resisting fatigue in mice, and can effectively solve the fatigue and metabolic decline caused by mitochondrial function degeneration, and the effect is significant and efficient.

[0057] Experimental Example 2: Efficacy stability determination test.

[0058] The experimental steps for determining the efficacy stability of the nutritional supplement composition for maintaining mitochondrial function prepared in Examples 1-3 of the present invention are as follows: (1) The nutritional supplement compositions for maintaining mitochondrial function prepared in Examples 1-3, the nutritional supplement compositions for maintaining mitochondrial function prepared in Comparative Examples 1-2, and a commercially available mitochondrial maintenance agent (purchased from Guangzhou Limeikang Biotechnology Co., Ltd.) in the control group were stored at a temperature of 28° C. and a humidity of 85% for 48 days to obtain the nutritional supplement compositions for maintaining mitochondrial function prepared in Examples 1-3 after storage, the nutritional supplement compositions for maintaining mitochondrial function prepared in Comparative Examples 1-2 after storage, and the commercially available mitochondrial maintenance agent in the control group after storage; (2) Sixty 8-week-old SPF mice weighing 25 g were selected (purchased from Chengdu Dashuo Animal Co., Ltd.) and randomly divided into 6 groups of 10 mice each. The mice were housed in separate cages, kept clean and dry, at a temperature of 24°C and a humidity of 50%, with free access to food and water. (3) After the mice in step (2) are anesthetized, the microcirculatory blood flow (PU) of the mouse auricle is measured using a laser Doppler microcirculatory instrument, and the result is recorded as the initial microcirculatory blood flow of the mouse auricle; (4) The nutritional supplement composition for maintaining mitochondrial function prepared in Example 1-3 after storage was fed to the mice in Example 1-3, with each mouse fed 2.5 mg per day. The nutritional supplement composition for maintaining mitochondrial function prepared in Comparative Example 1-2 after storage was fed to the mice in Comparative Example 1-2, with each mouse fed 2.5 mg per day. The commercially available mitochondrial maintenance agent for the control group after storage was fed to the mice in the control group, with each mouse fed 2.5 mg per day. Two hours after the mice in Example 1-3, Comparative Example 1-2 and Control Group were fed with the nutritional supplement composition for maintaining mitochondrial function prepared in Example 1-3 after storage, the nutritional supplement composition for maintaining mitochondrial function prepared in Comparative Example 1-2 after storage and the commercially available mitochondrial maintenance agent for the control group after storage, the blood flow of the mice was measured and the data was recorded. The difference in the microcirculation blood flow of the mice's auricles after storage was calculated according to the formula: the difference in the microcirculation blood flow of the mice's auricles after storage = the microcirculation blood flow of the mice's auricles after administration - the initial microcirculation blood flow of the mice's auricles; (5) Sixty 8-week-old SPF mice weighing 25 g were selected (purchased from Chengdu Dashuo Animal Co., Ltd.) and randomly divided into 6 groups of 10 mice each. The mice were housed in separate cages, kept clean and dry, at a temperature of 24°C and a humidity of 50%, with free access to food and water. (6) Place the mouse in step (5) on a roller with a rotation speed of 18 r / min and start pedaling. Record the number of times the mouse falls off the wheel due to muscle fatigue within 20 minutes, which is recorded as the initial number of wheel falls. (7) On the second day, the stored nutritional supplement composition for maintaining mitochondrial function prepared in Example 1-3 was fed to the mice in Group 1-3 at a rate of 2.5 mg per day. The stored nutritional supplement composition for maintaining mitochondrial function prepared in Comparative Example 1-2 was fed to the mice in Group 1-2 at a rate of 2.5 mg per day. The stored commercially available mitochondrial maintenance agent in the control group was fed to the mice in the control group at a rate of 2.5 mg per day. Three hours after feeding, the mice in each group were placed on a rolling wheel with a rotation speed of 18 r / min to start pedaling. The number of times the mice fell off the wheel due to muscle fatigue within 20 minutes was recorded as the number of times the wheel fell off after administration.

[0059] Result analysis: Figure 3This is a graph showing the difference in microcirculatory blood flow in the auricle of mice after storage as described in Experimental Example 2 of the present invention. As shown in the figure, the mice in groups 1-3 of Examples were fed with the nutritional supplement composition for maintaining mitochondrial function prepared in Examples 1-3 after storage, and each mouse was fed 2.5 mg every day for 2 hours. The differences in auricle microcirculation PU of the mice in groups 1-3 of Examples were 38.57, 40.46, and 41.57, respectively. The mice in groups 1-2 of Comparative Examples were fed with the nutritional composition for maintaining mitochondrial function prepared in Comparative Examples 1-2 after storage, and each mouse was fed 2.5 mg every day for 2 hours. The differences in auricle microcirculation PU of the mice in groups 1-2 of Comparative Examples were 16.62 and 29.46, respectively. The mice in the control group were fed with the commercially available mitochondrial curing agent in the control group after storage, and each mouse was fed 2.5 mg every day for 2 hours. The difference in auricle microcirculation PU of the mice in the control group was 8.35. Figure 4 This is a graph showing the number of times mice fell off the wheel after storage as described in Experimental Example 2 of the present invention. As shown in the figure, after 3 h of feeding and storage with the nutritional supplement composition for maintaining mitochondrial function prepared in Examples 1-3, the mice in Groups 1 to 3 of Examples 1 to 3 began to pedal on a roller with a rotation speed of 18 r / min, and the number of falls within 20 minutes decreased significantly and steadily. After 3 h of feeding and storage with the commercially available mitochondrial curing agent, the mice in Group 1 began to pedal on a roller with a rotation speed of 18 r / min, and the number of falls within 20 minutes showed little difference before and after comparison. This indicates that the nutritional supplement composition for maintaining mitochondrial function prepared in the present invention has a significant effect on promoting circulation and relieving fatigue, and its efficacy is stable and long-lasting after long-term use and storage.

[0060] Experimental Example 3: Irritation measurement test.

[0061] The irritation test steps for the nutritional supplement composition for maintaining mitochondrial function prepared in Examples 1-3 of the present invention are as follows: (1) Sixty male SD rats, each weighing 220 g (purchased from Chengdu Dashuo Animal Co., Ltd.), were randomly divided into eight groups of 10 rats each. The rats were housed in separate cages, which were kept clean and dry at a temperature of 24°C and a humidity of 50%, with free access to food and water. (2) By gavage, the SD male rats in Example 1-3 groups were respectively administered with the nutritional supplement composition for maintaining mitochondrial function prepared in Example 1-3, with each rat being fed 22 mg per day. The SD male rats in Comparative Example 1-2 groups were respectively administered with the nutritional supplement composition for maintaining mitochondrial function prepared in Comparative Example 1-2 groups, with each rat being fed 22 mg per day. The SD male rats in the control group were respectively administered with the commercially available mitochondrial maintenance agent (purchased from Guangzhou Limeikang Biotechnology Co., Ltd.), with each rat being fed 22 mg per day. After continuous gavage for 15 days, the diarrhea rate of the rats in each group on the 15th day was observed and recorded, and the average value was taken. The diarrhea rate of the rats was calculated according to the formula: diarrhea rate (%) = number of rats with diarrhea in each group / total number of rats in each group × 100%; Table 1. Rat irritation test table

[0062] Result analysis: As shown in Table 1, the SD male rats in groups 1-3 of Examples were gavaged with the nutritional supplement composition for maintaining mitochondrial function prepared in Examples 1-3 every day, the SD male rats in groups 1-2 of Comparative Examples were gavaged with the nutritional composition for maintaining mitochondrial function prepared in Groups 1-2 of Comparative Examples every day, and the SD male rats in the control group were gavaged with the commercially available mitochondrial maintaining agent of the control group every day. After 15 consecutive days of gavage, the diarrhea rates of the rats in groups 1-3 of Examples and groups 1-2 of Comparative Examples were 0%, while the diarrhea rate of the rats in the control group was 60%. This indicates that the nutritional supplement composition for maintaining mitochondrial function prepared by the present invention is non-irritating to the gastrointestinal tract when taken for a long time, has high bioavailability, and strong biocompatibility.

[0063] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

[0064] The present invention and its embodiments are described above. Such description is not restrictive. The drawings show only one embodiment of the present invention, and actual applications are not limited thereto. In short, if a person skilled in the art is inspired by the above, and does not deviate from the purpose of the present invention, any method and embodiment similar to the technical solution without creative design shall fall within the scope of protection of the present invention.

Claims

1. A nutritional supplement composition for maintaining mitochondrial function, characterized in that: The raw materials for preparing the nutritional supplement composition for maintaining mitochondrial function specifically include the following components in parts by weight: 18-22 parts of a mitochondrial nourishing nutritional composition, 11-13 parts of an agent for assisting in improving mitochondrial function, 1.5-2 parts of alpha-lipoic acid, 1.1-1.3 parts of hydroxytyrosol, 2.5-3.5 parts of cod collagen peptide, 1.2-1.4 parts of calcium pantothenate, and 0.8-1.2 parts of N-acetyl-L-cysteine; The raw materials for preparing the mitochondrial nourishing nutritional composition include the following components in parts by weight: 4-7 parts of fish collagen tripeptide, 1.1-1.4 parts of epigallocatechin gallate, 1.1-1.7 parts of nicotinamide riboside, 2.5-3.2 parts of grape seeds, 1.8-2.3 parts of pine bark, 2.2-2.4 parts of cyperus rotundus, 0.8-1.1 parts of selenomethionine, 0.7-0.9 parts of soybeans, 2.4-2.8 parts of black walnut kernels, 1.5-1.8 parts of saffron extract, 1.1-1.4 parts of verbena extract, 0.5-0.8 parts of disodium edetate, 0.7-1.2 parts of licorice extract, 0.6-0.9 parts of chitosan, 1.2-1.5 parts of pectin, 0.5-0.8 parts of coenzyme Q10, 0.3-0.6 parts of L-carnitine, and 0.7-0.9 parts of sodium octanoate; The raw materials for preparing the auxiliary agent for improving mitochondrial function include the following components in parts by weight: 2.2-2.6 parts of ginseng extract, 1.1-1.3 parts of spermidine, 0.4-0.8 parts of asiaticoside, 2.2-2.5 parts of guava leaves, 2.4-2.7 parts of red beans, 2.5-2.8 parts of eucommia, 3.2-3.6 parts of thyme, 1.1-1.3 parts of mixed probiotics, 2-2.5 parts of milk thistle seed oil, 0.8-1.2 parts of carvacrol, 1.1-1.3 parts of limonene, 0.3-0.6 parts of catechins, 0.4-0.7 parts of proanthocyanidins, 1.2-1.4 parts of emblica extract, and 0.5-0.8 parts of resveratrol.

2. The mixed probiotics consist of Lactobacillus plantarum, Lactobacillus rhamnosus, and Bifidobacterium longum subspecies longum, with a mixing ratio by weight of 0.9:1.1:

1.

3. The nutritional supplement composition for maintaining mitochondrial function according to claim 1, characterized in that: The preparation method of the mitochondrial maintenance nutritional composition specifically comprises the following steps: S1. Mixing fish collagen tripeptide, epigallocatechin gallate, and nicotinamide riboside to obtain a mixture for improving mitochondrial function; S2. Grind grape seeds, pine bark, and cyperus rotundus, extract with 70% ethanol solution, and mix with selenomethionine to obtain a plant extract composition; S3, grinding soybeans and black walnut kernels, and mixing them with saffron extract and verbena extract to obtain a regulating component; S4, mixing disodium EDTA, licorice extract, chitosan, pectin, and 2% acetic acid solution for reaction, and adding coenzyme Q10, L-carnitine, and sodium octanoate to obtain a carrier material; S5. Add the mixture for improving mitochondrial function prepared in S1, the plant extract composition prepared in S2, and the regulating component prepared in S3 into the carrier material prepared in S4, stir and mix, and freeze-dry to obtain a mitochondrial maintenance nutrient composition.

4. The nutritional supplement composition for maintaining mitochondrial function according to claim 2, characterized in that: The preparation method of the agent for assisting in improving mitochondrial function specifically comprises the following steps: L1. Mixing ginseng extract, spermidine, and asiaticoside to obtain a repair-promoting composition; L2. Grind guava leaves, red beans, eucommia bark, and thyme, then ferment them with mixed probiotics and ultrapure water, and add milk thistle seed oil, carvacrol, and limonene to obtain a fermentation product; L3, reacting catechins, proanthocyanidins, emblica fruit extract, resveratrol, and 50% ethanol solution to obtain a complex suspension; L4. Add the repair-promoting composition prepared by L1 and the fermentation product prepared by L2 into the complex suspension prepared by L3, stir and mix, and freeze-spray dry to obtain an agent that assists in improving mitochondrial function.

5. A method for preparing the nutritional supplement composition for maintaining mitochondrial function according to any one of claims 1 to 3, characterized in that: The specific steps include: Step 1: Mix α-lipoic acid, hydroxytyrosol, cod collagen peptide, calcium pantothenate, and N-acetyl-L-cysteine ​​to obtain a mild component that maintains activity; Step 2: Add the auxiliary agent for improving mitochondrial function into the active and mild component prepared in Step 1, and mix them to obtain a mixed component; Step 3: Evenly disperse the mitochondrial maintenance nutritional composition in the mixed components prepared in step 2 to obtain a nutritional supplement composition for maintaining mitochondrial function.

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